Cistanche deserticola health care wine as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of health-care wine, and particularly relates to cistanche health-care wine as well as a preparation method and application thereof. The cistanche health care wine is prepared from the following active ingredients: 40 mg / 100 g to 110 mg / 100 g of phenylethanoid glycosides and 4 mg / 100 g to 15 mg / 100 g of iridoid. The cistanche health-care wine provided by the invention is simple in component and definite in effect, has a relatively good fatigue relieving effect, and is suitable for ordinary people to take daily.

Description

Technical Field

[0001] The present invention belongs to the technical field of health care wine, and particularly relates to a cistanche salsa health care wine, a preparation method thereof and an application thereof. Background Art

[0002] With the continuous improvement of living standards, people have higher and higher demands for health, and there are also many demands for health preservation and health care before getting sick. Health care wine is a kind of "beverage wine" formed by using distilled wine or fermented wine as the base of the wine and adding health care parts or components of animals and plants; it is completely different from ordinary white wine and belongs to the category of health care series. The various health care parts or components added during the brewing process of health care wine have specific pharmacological requirements and compatibility considerations for medication. According to their functions, they can be divided into types such as replenishing qi, nourishing blood, nourishing yin, tonifying yang, and tonifying both qi and blood. However, no matter which of the above functions, the ultimate goal is to achieve health preservation, physical fitness, and strengthening the body.

[0003] Cistanche salsa, also known as Cistanche deserticola, is a parasitic plant that parasitizes on the roots of desert trees Haloxylon ammodendron. It absorbs nutrients and water from the host Haloxylon ammodendron, and is known as the "ginseng in the desert" and has extremely high medicinal value. It is a traditional precious Chinese medicinal material in China.

[0004] The prior art discloses an anti-fatigue health care wine, which comprises the following raw materials in parts by weight: 0.5 - 3 parts of fennel, 0.8 - 2 parts of cistanche salsa, 0.5 - 1.3 parts of poria cocos, 0.3 - 2.1 parts of alisma orientale, 0.2 - 1 part of liquorice, 0.5 - 1.6 parts of red dates, 0.6 - 1.3 parts of codonopsis pilosula, 0.5 - 1.8 parts of cynomorium songaricum, 0.5 - 1.5 parts of walnuts, 1 - 3 parts of wolfberries, 6 - 13 parts of white sugar, and 150 - 300 parts of white liquor. The raw materials of this health care wine are numerous, the formula is complex, and the stability is poor. A large amount of flocculent precipitation will occur during the shelf life, seriously affecting the appearance, resulting in consumers giving up the choice, and also increasing the costs of the enterprise for selecting materials and inspecting and warehousing. Secondly, the cynomorium songaricum raw material in this product does not belong to the raw materials of both food and medicine, resulting in that the formula of this product can only be declared as a health food. However, such a product with a complex formula does not conform to the formula design principle for health food declaration, thus exacerbating the market launch risk and difficulty of the product. Summary of the Invention

[0005] In view of this, the present invention provides a cistanche salsa health care wine, which has simple ingredients, good stability and remarkable anti-fatigue effect.

[0006] The present application also provides a preparation method of a cistanche salsa health care wine. This method only selects four raw materials of both food and medicine, and after simple treatment, it is soaked in wine to obtain a cistanche salsa health care wine with good stability and good anti-fatigue effect.

[0007] According to an embodiment of the present application, in the first aspect, there is provided a cistanche salsa health care wine, which comprises the following active ingredients:

[0008] Phenylethanoid glycosides 40 mg / 100 g - 110 mg / 100 g; and

[0009] Iridoids 4 mg / 100 g - 15 mg / 100 g.

[0010] Optionally, the cistanche health wine further comprises the following active ingredients:

[0011] Total saponins 16 mg / 100 g - 42 mg / 100 g;

[0012] Total flavonoids 320 mg / 100 g - 830 mg / 100 g; and

[0013] Crude polysaccharides 26 mg / 100 g - 42 mg / 100 g.

[0014] Optionally, by weight, the raw material composition of the cistanche health wine comprises:

[0015]

[0016]

[0017] Optionally, the wine is white liquor with an alcohol content of 30 - 80 degrees.

[0018] Further, the white liquor is 190 - 250 parts, and the alcohol content of the white liquor is 65 degrees.

[0019] Further, by weight, the raw material composition of the cistanche health wine comprises:

[0020]

[0021] According to an embodiment of the present invention, in a second aspect, the present invention provides a method for preparing a cistanche health wine, comprising the following steps:

[0022] Pre-treat cistanche under the conditions of 3 - 7 atmospheres, 60 - 70 °C, and a humidity of 24.2% - 37%, then soak it in wine and stir once at an interval of the first time to obtain a cistanche wine solution;

[0023] Crush American ginseng, then soak it in wine and stir once at an interval of the first time to obtain an American ginseng wine solution;

[0024] Crush cordyceps militaris, then soak it in wine and stir once at an interval of the first time to obtain a cordyceps militaris wine solution;

[0025] Crush kudzu root, then soak it in wine and stir once at an interval of the first time to obtain a kudzu root wine solution;

[0026] It is obtained by mixing the cistanche deserticola wine, American ginseng wine, cordyceps militaris wine, and kudzu root wine.

[0027] Optionally, cordyceps militaris and / or kudzu root need to be steamed for at least 30 minutes before being crushed.

[0028] Optionally, the first time is at least 30 days.

[0029] Optionally, the stirring time is at least 30 minutes.

[0030] Optionally, the stirring is intermittent stirring, and the number of stirring times in each intermittent stirring cycle is 100 - 500 times.

[0031] Optionally, the mass ratios of cistanche deserticola, American ginseng, cordyceps militaris, and kudzu root to wine are respectively 1:(2 - 30).

[0032] According to an embodiment of the present invention, in a third aspect, the present invention also provides the application of the cistanche deserticola health wine described in the first aspect or the cistanche deserticola health wine prepared by the preparation method described in the second aspect in relieving physical fatigue.

[0033] The technical solution of the present invention has the following advantages:

[0034] 1. The cistanche deserticola health wine provided by the present invention contains the following active ingredients: phenyl ethanol glycosides 40mg / 100g - 110mg / 100g and iridoids 4mg / 100g - 15mg / 100g. Research shows that phenyl ethanol glycosides have the effects of relieving physical fatigue and improving muscle state. The biological activities of iridoids are extensive, mainly manifested as anti - inflammation, antioxidant, anti - tumor, and neuroprotection, etc. By increasing the content of phenyl ethanol glycosides, the anti - fatigue function of the health wine can be improved. At the same time, the presence of an appropriate amount of iridoids can play an antioxidant effect, which can maintain the color and flavor of the wine body for a long time, and at the same time maintain the stability of the wine body during the shelf life, thereby maintaining the taste and extending the shelf life.

[0035] Furthermore, the cistanche health wine provided by the present invention also contains the following active ingredients: total saponins 16 mg / 100 g - 42 mg / 100 g, total flavonoids 320 mg / 100 g - 830 mg / 100 g, and crude polysaccharides 26 mg / 100 g - 42 mg / 100 g. Flavonoids have a definite anti-fatigue activity, and flavonoids are commonly used as a marker component for anti-fatigue in health foods. The high content of total flavonoids in the health wine of the present invention indicates its good anti-fatigue effect. Saponins generally have the effects of antioxidation, anti-inflammation, enhancing immunity, regulating blood sugar, and protecting the liver. The presence of an appropriate amount of saponins in the health wine of the present invention can improve the oxidative damage of the body after exercise by means of antioxidation, anti-inflammation, etc., reduce the generation of oxidation products, and prevent the accumulation of lactic acid, thereby playing a role in relieving physical fatigue. Crude polysaccharides can enhance immunity, regulate blood lipid, and lower blood sugar. In combination with phenylpropanoid glycosides, iridoids, etc., they can act on the expression of phosphatidylinositol 3-kinase, protein kinase B, and glycogen synthase kinase-3 in skeletal muscle, thereby reducing the accumulation of metabolites, promoting glycogen synthesis, and playing a role in relieving physical fatigue.

[0036] Therefore, the cistanche health wine of the present application has the effect of relieving physical fatigue, and its ingredients are simple, the liquid is clear, and its stability is high. There will be no large amount of flocculants during the shelf life, which will damage the beauty of the product and then affect the purchase desire of consumers.

[0037] 2. The raw material composition of the cistanche health wine provided by the present invention includes 6 - 30 parts of cistanche, 10 - 30 parts of American ginseng, 5 - 20 parts of cordyceps militaris, 10 - 20 parts of kudzu root, and 100 - 260 parts of wine. The formula of the present invention is simple, the efficacy is clear, and it conforms to the principle of formula compatibility design of health foods. All the raw materials used in the formula have a definite anti-fatigue effect. After being formulated, flavonoids, saponins, polysaccharides, etc. can relieve physical fatigue through multiple different action mechanisms such as regulating carbohydrate metabolism, activating phosphatidylinositol-3-kinase (PI3K) / protein kinase B (Akt) / mammalian target of rapamycin (mTOR), improving the energy supply efficiency of skeletal muscle mitochondria, and accelerating the scavenging of free radicals, which is superior to products with a single component and a single action mechanism, and plays a synergistic effect to maximize the anti-fatigue effect of agricultural products. Moreover, all the raw materials used in the present invention belong to raw materials that are both food and medicine. Therefore, there are more choices for listing. It can not only be applied for listing as a health food, but also can be applied for listing and selling functional foods immediately, which undoubtedly improves the product layout and market share of the enterprise. At the same time, the simple formula also reduces the burden of in-warehouse inspection and regular verification of the enterprise.

[0038] 3. The preparation method of the cistanche deserticola health wine provided by the present invention is as follows: the raw materials such as American ginseng, cordyceps militaris, and pueraria lobata are respectively washed, crushed, put into a tank, soaked in wine, stirred and left standing to obtain three kinds of original juices. After the cistanche deserticola is washed, it is first subjected to constant temperature and humidity treatment, and then crushed, put into a tank, soaked in wine, stirred and left standing to obtain the original juice of cistanche deserticola; then the various original juices are mixed, left standing, and filtered to obtain the finished wine. Since the cistanche deserticola has the characteristic that the content of active ingredients slowly increases in a special environment after being dug, the fresh cistanche deserticola contains a high water and sugar content. If it is naturally dried, it will take a long time, and at the same time, the content of phenyl ethanol glycosides will be reduced, and the cistanche deserticola with a high water content is prone to mildew. Therefore, in the present invention, by subjecting the cistanche deserticola to constant temperature and humidity treatment, rapid drying, and controlling the humidity during the drying process, the drying time can be greatly shortened, the phenyl ethanol glycoside components can be stabilized, and the active ingredients can be maximally retained, thereby ensuring the good anti-fatigue effect of the health wine of the present invention.

[0039] Further, before crushing cordyceps militaris and / or pueraria lobata, they are steamed for at least 30 minutes, thereby improving the bioactive components in cordyceps militaris and / or pueraria lobata, including crude polysaccharides, puerarin, total flavonoids and other components, and thus improving the anti-fatigue effect of the cistanche deserticola health wine. Specific embodiments

[0040] The technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0041] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application are intended to cover non-exclusive inclusion.

[0042] In the embodiments and comparative examples of the present invention, the unit "part" represents parts by weight. It can be understood that 1 part by weight can be 1 g, or any mass such as 10 g, 50 g, 100 g, 500 g, 1000 g, etc.

[0043] Example 1

[0044] A cistanche deserticola health wine, the raw material composition of which includes: 6 parts of cistanche deserticola, 10 parts of American ginseng, 10 parts of cordyceps militaris, 10 parts of pueraria lobata, and 108 parts of white wine (alcohol content is 65 degrees).

[0045] The cistanche health wine of this embodiment is prepared by the following preparation method, and the specific steps are as follows:

[0046] Raw material treatment:

[0047] Take 6 g of cistanche for cleaning. After cleaning, it is processed in a constant temperature and humidity environment, where the temperature is 60 °C, the humidity is 25%, and the pressure is 3 atmospheres; after processing, it is further pulverized, and after pulverization, it is put into an immersion tank, and 18 g of wine is added to it for immersion. Stir for 30 minutes every month and immerse for 6 months;

[0048] Take 10 g of American ginseng for cleaning. After cleaning, it is pulverized, and after pulverization, it is put into an immersion tank, and 30 g of wine is added to the pulverized American ginseng for immersion. Stir for 30 minutes every month and immerse for 6 months;

[0049] Take 10 g of cordyceps militaris for cleaning. The cleaned cordyceps militaris is steamed for 30 minutes, and after steaming, it is pulverized. After pulverization, it is put into an immersion tank, and 30 g of wine is added to the pulverized cordyceps militaris for immersion. Stir for 30 minutes every month and immerse for 6 months;

[0050] Take 10 g of kudzu root for cleaning. The cleaned kudzu root is steamed for 30 minutes, and after steaming, it is pulverized. After pulverization, it is put into an immersion tank, and 30 g of wine is added to the pulverized kudzu root for immersion. Stir for 30 minutes every month and immerse for 6 months;

[0051] Merging of the stock solutions: Just mix the wine solutions obtained by processing different raw materials in the raw material treatment step.

[0052] Example 2

[0053] A cistanche health wine, the raw material composition of which includes: 6 parts of cistanche, 20 parts of American ginseng, 20 parts of cordyceps militaris, 15 parts of kudzu root, and 183 parts of white liquor (alcohol content is 80 degrees).

[0054] The cistanche health wine of this embodiment is prepared by the following preparation method, and the specific steps are as follows:

[0055] Raw material treatment:

[0056] Take 6 g of cistanche for cleaning. After cleaning, it is further processed in a constant temperature and humidity environment, where the temperature is 70 °C, the humidity is 24.2%, and the pressure is 3 atmospheres; after processing, it is further pulverized, and after pulverization, it is put into an immersion tank, and 18 g of wine is added to it for immersion. Stir for 30 minutes every month and immerse for 6 months;

[0057] Take 20 g of American ginseng for cleaning, crush it after cleaning, put the crushed American ginseng into an immersion tank, add 60 g of wine to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months;

[0058] Take 20 g of Cordyceps militaris for cleaning, steam the cleaned Cordyceps militaris for 60 minutes, crush it after steaming, add 60 g of wine to the crushed Cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months;

[0059] Take 15 g of Pueraria lobata for cleaning, steam the cleaned Pueraria lobata for 45 minutes, crush it after steaming, add 45 g of wine to the crushed Pueraria lobata for soaking, stir for 30 minutes every month, and soak for 6 months;

[0060] Merger of stock solutions: Just mix the wine solutions obtained after treating different raw materials in the raw material treatment step.

[0061] Example 3

[0062] A Cistanche deserticola health wine, the raw material composition of which includes: 15 parts of Cistanche deserticola, 20 parts of American ginseng, 20 parts of Cordyceps militaris, 15 parts of Pueraria lobata, and 210 parts of white wine (alcohol content is 50 degrees).

[0063] The Cistanche deserticola health wine of this example is prepared by the following preparation method, and the specific steps are as follows:

[0064] Raw material treatment:

[0065] Take 15 g of Cistanche deserticola for cleaning, and conduct further treatment in a constant temperature and humidity environment, where the temperature is 68 °C, the humidity is 35%, and the pressure is 5 atmospheres; after treatment, further crush it, put the crushed Cistanche deserticola into an immersion tank, add 45 g of wine to it for soaking, stir for 30 minutes every month, and soak for 6 months;

[0066] Take 20 g of American ginseng for cleaning, crush it after cleaning, put the crushed American ginseng into an immersion tank, add 60 g of wine to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months;

[0067] Take 20 g of Cordyceps militaris for cleaning, steam the cleaned Cordyceps militaris for 45 minutes, crush it after steaming, add 60 g of wine to the crushed Cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months;

[0068] Take 15 g of Pueraria lobata for cleaning, steam the cleaned Pueraria lobata for 60 minutes, crush it after steaming, add 45 g of wine to the crushed Pueraria lobata for soaking, stir for 30 minutes every month, and soak for 6 months;

[0069] Stock solution merging: Just mix the liquor obtained after processing different raw materials in the raw material processing step.

[0070] Example 4

[0071] A cistanche deserticola health care liquor, whose raw material composition includes: 20 parts of cistanche deserticola, 20 parts of American ginseng, 10 parts of cordyceps militaris, 15 parts of kudzu root, and 195 parts of white liquor (alcohol content is 65 degrees).

[0072] The cistanche deserticola health care liquor of this example is prepared by the following preparation method, and the specific steps are as follows:

[0073] Raw material processing:

[0074] Take 20 g of cistanche deserticola for cleaning, and after cleaning, conduct further processing in a constant temperature and humidity environment, where the temperature is 60 °C, the humidity is 30%, and the pressure is 7 atmospheres; after processing, further crush it, and after crushing, put it into an immersion tank, add 60 g of liquor to it for soaking, stir for 30 minutes every month, and soak for 6 months;

[0075] Take 20 g of American ginseng for cleaning, and after cleaning, crush it. After crushing, put it into an immersion tank, add 60 g of liquor to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months;

[0076] Take 10 g of cordyceps militaris for cleaning, steam the cleaned kudzu root for 30 minutes, and after steaming, crush it. Add 30 g of liquor to the crushed cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months;

[0077] Take 15 g of kudzu root for cleaning, steam the cleaned cordyceps militaris for 30 minutes, and after steaming, crush it. Add 45 g of liquor to the crushed kudzu root for soaking, stir for 30 minutes every month, and soak for 6 months;

[0078] Stock solution merging: Just mix the liquor obtained after processing different raw materials in the raw material processing step.

[0079] Example 5

[0080] A cistanche deserticola health care liquor, whose raw material composition includes: 10 parts of cistanche deserticola, 10 parts of American ginseng, 20 parts of cordyceps militaris, 10 parts of kudzu root, and 150 parts of white liquor (alcohol content is 40 degrees).

[0081] The cistanche deserticola health care liquor of this example is prepared by the following preparation method, and the specific steps are as follows:

[0082] Raw material processing:

[0083] Take 10 g of Cistanche deserticola for cleaning. After cleaning, conduct further treatment in a constant temperature and humidity environment, with a temperature of 70 °C, a humidity of 30%, and a pressure of 3 atmospheres. After treatment, further crush it, and after crushing, put it into a soaking tank, add 30 g of wine for soaking, stir for 30 minutes every month, and soak for 6 months.

[0084] Take 10 g of American ginseng for cleaning. After cleaning, crush it. After crushing, put it into a soaking tank, add 30 g of wine to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months.

[0085] Take 20 g of Cordyceps militaris for cleaning. Steam the cleaned Cordyceps militaris for 60 minutes. After steaming, crush it. Add 60 g of wine to the crushed Cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months.

[0086] Take 10 g of Pueraria lobata for cleaning. Steam the cleaned Pueraria lobata for 60 minutes. After steaming, crush it. Add 30 g of wine to the crushed Pueraria lobata for soaking, stir for 30 minutes every month, and soak for 6 months.

[0087] Stock solution merging: Just mix the wine liquids obtained after treating different raw materials in the raw material treatment steps.

[0088] Example 6

[0089] A Cistanche deserticola health wine, whose raw material composition includes: 10 parts of Cistanche deserticola, 10 parts of American ginseng, 20 parts of Cordyceps militaris, 10 parts of Pueraria lobata, and 250 parts of white liquor (alcohol content is 65 degrees).

[0090] The Cistanche deserticola health wine of this example is prepared by the following preparation method, and the specific steps are as follows:

[0091] Raw material treatment:

[0092] Take 10 g of Cistanche deserticola for cleaning. After cleaning, conduct further treatment in a constant temperature and humidity environment, where the temperature is 60 °C, the humidity is 37%, and the pressure is 6 atmospheres. After treatment, further crush it, and after crushing, put it into a soaking tank, add 50 g of wine for soaking, stir for 30 minutes every month, and soak for 6 months.

[0093] Take 10 g of American ginseng for cleaning. After cleaning, crush it. After crushing, put it into a soaking tank, add 50 g of wine to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months.

[0094] Take 20 g of Cordyceps militaris for cleaning, steam the cleaned Cordyceps militaris for 30 minutes, crush it after steaming, add 100 g of wine to the crushed Cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months;

[0095] Take 10 g of Pueraria lobata for cleaning, steam the cleaned Pueraria lobata for 30 minutes, crush it after steaming, add 50 g of wine to the crushed Pueraria lobata for soaking, stir for 30 minutes every month, and soak for 6 months;

[0096] Stock solution merging: Just mix the wine solutions obtained by treating different raw materials in the raw material treatment step.

[0097] Example 7

[0098] A cistanche health wine, the raw material composition of which includes: 15 parts of cistanche, 13 parts of American ginseng, 12 parts of Cordyceps militaris, 17 parts of Pueraria lobata, and 171 parts of white wine (alcohol content is 30 degrees).

[0099] The cistanche health wine of this example is prepared by the following preparation method, and the specific steps are as follows:

[0100] Raw material treatment:

[0101] Take 15 g of cistanche for cleaning, and conduct further treatment in a constant temperature and humidity environment after cleaning, where the temperature is 63 °C, the humidity is 33.2%, and the pressure is 5 atmospheres; after treatment, further crush it, put it into a soaking tank after crushing, add 45 g of wine to it for soaking, stir for 30 minutes every month, and soak for 6 months;

[0102] Take 13 g of American ginseng for cleaning, crush it after cleaning, put it into a soaking tank after crushing, add 39 g of wine to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months;

[0103] Take 12 g of Cordyceps militaris for cleaning, steam the cleaned Cordyceps militaris for 45 minutes, crush it after steaming, add 36 g of wine to the crushed Cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months;

[0104] Take 17 g of Pueraria lobata for cleaning, steam the cleaned Pueraria lobata for 45 minutes, crush it after steaming, add 51 g of wine to the crushed Pueraria lobata for soaking, stir for 30 minutes every month, and soak for 6 months;

[0105] Stock solution merging: Just mix the wine solutions obtained by treating different raw materials in the raw material treatment step.

[0106] Example 8

[0107] A cistanche deserticola health care wine, the raw material composition of which includes: 8 parts of cistanche deserticola, 12 parts of American ginseng, 14 parts of cordyceps militaris, 14 parts of kudzu root, and 158 parts of white liquor (alcohol content is 65 degrees).

[0108] The cistanche deserticola health care wine of this embodiment is prepared by the following preparation method, and the specific steps are as follows:

[0109] Raw material treatment:

[0110] Take 8g of cistanche deserticola for cleaning, and after cleaning, conduct further treatment in a constant temperature and humidity environment, where the temperature is 62 °C, the humidity is 30%, and the pressure is 3 atmospheres; after treatment, further crush it, and after crushing, put it into an immersion tank, add 40g of wine to it for soaking, stir for 30 minutes every month, and soak for 6 months;

[0111] Take 12g of American ginseng for cleaning, and after cleaning, crush it. After crushing, put it into an immersion tank, add 36g of wine to the crushed American ginseng for soaking, stir for 30 minutes every month, and soak for 6 months;

[0112] Take 14g of cordyceps militaris for cleaning, steam the cleaned cordyceps militaris for 50 minutes, and after steaming, crush it. Add 42g of wine to the crushed cordyceps militaris for soaking, stir for 30 minutes every month, and soak for 6 months;

[0113] Take 14g of kudzu root for cleaning, steam the cleaned kudzu root for 50 minutes, and after steaming, crush it. Add 42g of wine to the crushed kudzu root for soaking, stir for 30 minutes every month, and soak for 6 months;

[0114] Merger of stock solutions: Just mix the wine solutions obtained by treating different raw materials in the raw material treatment step.

[0115] Example 9

[0116] A cistanche deserticola health care wine, the raw material composition of which includes: 30 parts of cistanche deserticola, 11 parts of American ginseng, 18 parts of cordyceps militaris, 20 parts of kudzu root, and 187 parts of white liquor (alcohol content is 65 degrees).

[0117] The cistanche deserticola health care wine of this embodiment is prepared by the following preparation method, and the specific steps are as follows:

[0118] Raw material treatment:

[0119] Take 30g of cistanche deserticola for cleaning, and after cleaning, conduct further treatment in a constant temperature and humidity environment, where the temperature is 65 °C, the humidity is 30%, and the pressure is 3 atmospheres; after treatment, further crush it, and after crushing, put it into an immersion tank, add 60g of wine to it for soaking, stir for 30 minutes every month, and soak for 6 months;

[0120] Take 11 g of American ginseng for cleaning. After cleaning, it is pulverized. After pulverization, it is loaded into a soaking tank. Add 33 g of wine to the pulverized American ginseng for soaking. Stir for 30 minutes every month and soak for 6 months.

[0121] Take 18 g of Cordyceps militaris for cleaning. Steam the cleaned Cordyceps militaris for 40 minutes. After steaming, it is pulverized. Add 54 g of wine to the pulverized Cordyceps militaris for soaking. Stir for 30 minutes every month and soak for 6 months.

[0122] Take 20 g of Pueraria lobata for cleaning. Steam the cleaned Pueraria lobata for 40 minutes. After steaming, it is pulverized. Add 40 g of wine to the pulverized Pueraria lobata for soaking. Stir for 30 minutes every month and soak for 6 months.

[0123] Combination of stock solutions: Just mix the wine solutions obtained by treating different raw materials in the raw material treatment step.

[0124] Example 10

[0125] A cistanche health wine, the raw material composition of which includes: 20 parts of cistanche, 30 parts of American ginseng, 5 parts of Cordyceps militaris, 10 parts of Pueraria lobata, and 210 parts of white liquor (alcohol content is 65 degrees).

[0126] The cistanche health wine of this example is prepared by the following preparation method, and the specific steps are as follows:

[0127] Raw material treatment:

[0128] Take 20 g of cistanche for cleaning. After cleaning, it is further processed in a constant temperature and humidity environment, where the temperature is 60 °C, the humidity is 28%, and the pressure is 3 atmospheres; after processing, it is further pulverized. After pulverization, it is loaded into a soaking tank. Add 60 g of wine to it for soaking. Stir for 30 minutes every month and soak for 6 months.

[0129] Take 30 g of American ginseng for cleaning. After cleaning, it is pulverized. After pulverization, it is loaded into a soaking tank. Add 90 g of wine to the pulverized American ginseng for soaking. Stir for 30 minutes every month and soak for 6 months.

[0130] Take 5 g of Cordyceps militaris for cleaning. Steam the cleaned Cordyceps militaris for 30 minutes. After steaming, it is pulverized. Add 30 g of wine to the pulverized Cordyceps militaris for soaking. Stir for 30 minutes every month and soak for 6 months.

[0131] Take 10 g of Pueraria lobata for cleaning. Steam the cleaned Pueraria lobata for 30 minutes. After steaming, it is pulverized. Add 30 g of wine to the pulverized Pueraria lobata for soaking. Stir for 30 minutes every month and soak for 6 months.

[0132] Combination of stock solutions: Just mix the liquor obtained after treating different raw materials in the raw material treatment step.

[0133] Comparative Example 1

[0134] Except for the following content, the rest is the same as in Example 4.

[0135] The raw material composition of the cistanche health wine in this comparative example includes: 40 parts of cistanche, 10 parts of cordyceps militaris, 15 parts of pueraria lobata, and 195 parts of white liquor.

[0136] That is, in Comparative Example 1, an equal mass of cistanche is used to replace American ginseng in Example 4.

[0137] Comparative Example 2

[0138] Except for the following content, the rest is the same as in Example 4.

[0139] The raw material composition of the cistanche health wine in this comparative example includes: 35 parts of cistanche, 20 parts of American ginseng, 10 parts of cordyceps militaris, and 195 parts of white liquor.

[0140] That is, in Comparative Example 2, an equal mass of cistanche is used to replace pueraria lobata in Example 4.

[0141] Comparative Example 3

[0142] Except for the following content, the rest is the same as in Example 4.

[0143] The raw material composition of the cistanche health wine in this comparative example includes: 30 parts of cistanche, 20 parts of American ginseng, 15 parts of pueraria lobata, and 195 parts of white liquor.

[0144] That is, in Comparative Example 3, an equal mass of cistanche is used to replace cordyceps militaris in Example 4.

[0145] Comparative Example 4

[0146] Except for the following content, the rest is the same as in Example 4.

[0147] After washing cistanche, directly crush it and soak it in liquor.

[0148] Comparative Example 5

[0149] Except for the following content, the rest is the same as in Example 4.

[0150] After washing cordyceps militaris, directly crush it and soak it in liquor.

[0151] Comparative Example 6

[0152] Except for the following content, the rest is the same as in Example 4.

[0153] After washing pueraria lobata, directly crush it and soak it in liquor.

[0154] Test example

[0155] Test example 1: Sensory test

[0156] The sensory properties of the Cistanche deserticola health wine prepared in Examples 1-10 were tested:

[0157] The sensory requirements are specified as:

[0158] Item Requirement Color and luster Light brown Taste and odor Have the unique taste and odor of Chinese herbal medicines, and be dense in the mouth State Clear liquid. A small amount of precipitation is allowed after standing for a long time and can be dispersed after shaking

[0159] Test results: The color of the Cistanche deserticola health wine prepared in Examples 1-10 was light brown clear liquid, and no obvious precipitate was observed after 24 months of storage.

[0160] Test example 2: Physicochemical index test

[0161] The physicochemical indexes of the Cistanche deserticola health wine prepared in Examples 1-10 were detected.

[0162] Physicochemical indexes:

[0163] Item Index Lead (calculated as Pb), mg / kg ≤0.5 Total arsenic (calculated as As), mg / kg ≤0.2 Methanol / (g / L) ≤2.0 Cyanide (calculated as HCN) / (mg / L) ≤8.0 BHC (hexachlorocyclohexane), mg / kg ≤0.1 DDT (dichlorodiphenyltrichloroethane), mg / kg ≤0.1

[0164] Detection methods for physicochemical indexes:

[0165] The detection method for lead (calculated as Pb) is: the first method in GB 5009.12-2017;

[0166] The detection method for total arsenic (calculated as As) is: the second method in the first part of GB 5009.11-2014;

[0167] The detection method for methanol is: detected by the detection method disclosed in Section 4.3 of GB / T 5009.48-2003;

[0168] The detection method for cyanide (calculated as HCN) is: detected by the detection method disclosed in Section 4.7 of GB / T 5009.48-2003;

[0169] The detection method for BHC is: the first method in GB / T 5009.19-2008;

[0170] The detection method for DDT is: the first method in GB / T 5009.19-2008.

[0171] Detection results: The lead content in the Cistanche deserticola health wine prepared in Examples 1-10 was less than 0.5 mg / kg, the total arsenic content was less than 0.2 mg / kg, the methanol content was less than 2.0 g / L, the cyanide content was less than 8.0 mg / L, the BHC content was less than 0.1 mg / kg, and the DDT content was less than 0.1 mg / kg.

[0172] Test Example 3 Active Ingredient Test

[0173] Perform active ingredient tests on the Cistanche deserticola health wine prepared in Examples 1, 2, 4, 6, and 8:

[0174] The detection method for total saponins is: the second method disclosed in Section XIV, Determination of Total Saponins in Health Foods, of the Technical Guidelines for the Inspection and Evaluation of the Physicochemical and Hygienic Indicators of Health Foods (2020 Edition);

[0175] The detection method for total flavonoids is: the second method disclosed in Section XIV, Determination of Total Flavonoids in Health Foods, of the Technical Guidelines for the Inspection and Evaluation of the Physicochemical and Hygienic Indicators of Health Foods (2020 Edition);

[0176] The detection method for crude polysaccharides is: Measure using Method 1, "Phenol-Sulfuric Acid Spectrophotometric Determination of Crude Polysaccharides", disclosed in the Detection Methods of Efficacy Components of Health Foods, Section 1, Detection of Polysaccharides;

[0177] The detection method for phenyl ethanol glycoside compounds is: Phenyl ethanol glycoside compounds include acteoside, verbascoside, isoverbascoside, 2'-acetyl verbascoside, tubuloside A, tubuloside B, and cistanoside A. The content of each of the above components is detected using the detection method in Simultaneous Determination of the Contents of 8 Components in Cistanche deserticola Slices by HPLC Method.

[0178] The detection method for iridoids is: Iridoids include geniposidic acid and 8-epiloganic acid (8-epistrychnic acid). The content of each of the above components is detected using the detection method in Simultaneous Determination of the Contents of 8 Components in Cistanche deserticola Slices by HPLC Method.

[0179] The detection results are shown in the following table:

[0180]

[0181]

[0182] As can be seen from the above table, the content ranges of each active ingredient in the Cistanche deserticola health wine prepared in Examples 1, 2, 4, 6, and 8 are: total saponins 16.87 mg / 100 g - 40.52 mg / 100 g; total flavonoids 320 mg / 100 g - 830 mg / 100 g; crude polysaccharides 26.54 mg / 100 g - 40.96 mg / 100 g; phenyl ethanol glycoside compounds 41.37 mg / 100 g - 109.37 mg / 100 g; iridoids 4.21 mg / 100 g - 14.04 mg / 100 g.

[0183] Combined with the above data, it can be seen that compared with other embodiments, the contents of each active ingredient in Example 4 are the highest. And compared with Example 4, in Comparative Examples 1-3, equal masses of Cistanche deserticola were used to replace American ginseng, Pueraria lobata, and Cordyceps militaris in the formula of Example 4, resulting in a higher content of phenyl ethanol glycosides in the health care wine prepared in Comparative Examples 1-3 than in Example 4. However, the contents of iridoids, total saponins, total flavonoids, and crude polysaccharides in Comparative Examples 1-3 are still lower than those in Example 4, indicating that there is a synergistic effect among the raw materials in Example 4. And compared with Example 4, the contents of each active ingredient in Comparative Examples 4-6 are significantly reduced. It can be seen that the formula and preparation method of Example 4 are both optimal, and there is a synergistic effect among the raw materials, indicating that the types and ratios of the raw materials in the product formula are reasonable and have certain advantages.

[0184] Test Example 4 Animal Experiment

[0185] Animal experiments were conducted on the cistanche health care wine prepared in Examples 1, 2, 4, 6, and 8.

[0186] The specific experimental method is as follows:

[0187] I. Materials and Methods

[0188] 1. Samples:

[0189] The cistanche health care wine prepared in Examples 1, 2, 4, 6, and 8 is a light brown transparent liquid with an alcohol content of 32±1% (V / V). The sample specification is: 500 mL / bottle. It is sealed and stored in a cool and dry place, with a shelf life of 24 months.

[0190] 2. Animal Experiment:

[0191] 306 Kunming strain clean-grade male mice weighing 18 g - 22 g bred by Beijing Huafukang Biotechnology Co., Ltd. [License number: SCXK-(Jing) 2014-0004] were selected and divided into four batches for the experiment. Each batch was randomly divided into 6 groups. Among them, groups 1-5 were respectively gavaged with the cistanche health care wine prepared in Examples 1, 2, 4, 6, and 8, group 6 was the blank control group, and groups 7-12 were respectively gavaged with the cistanche health care wine prepared in Comparative Examples 1-6. There were 15 mice in each group in the first batch of the experiment, and 12 mice in each group in the remaining three batches. The first batch of the experiment was for weight-bearing swimming, the second batch was for the determination of serum urea, the third batch was for the determination of liver glycogen, and the fourth batch was for the determination of blood lactic acid.

[0192] 3. Instruments and Reagents:

[0193] Instruments: UV1700 spectrophotometer (2014009), VITALAB SELECTRA-E biochemical analyzer (2005001), HZF-B3000 electronic balance (2013002), BS2202S electronic balance (2014008), BS223S electronic balance (2008007), centrifuge, oscillator, swimming box, lead wire, pipette, homogenizer, test tube, stoppered test tube.

[0194] Reagents: Urea kit (Sino-Biotech Co., Ltd., batch number: 141161), trichloroacetic acid (Tianjin Guangfu Fine Chemical Research Institute, batch number: 2013.4.7), glucose. 2 O (Amresco, batch number: 2734B53), sulfuric acid (Beijing Chemical Plant, batch number: 20140211, 20141109), anthrone (Shanghai Chemical Reagent Company of China Pharmaceutical Group, batch number: 20031203), thiourea (Sinopharm Chemical Reagent Co., Ltd., batch number: 20140424), copper sulfate (Beijing Chemical Plant, batch number: 20120826), sodium fluoride (Beijing Chemical Plant, batch number: 20100312), sodium tungstate (Tianjin Fuchen Chemical Reagent Factory, batch number: 20110527), p-hydroxybiphenyl (Sinopharm Chemical Reagent Co., Ltd., batch number: 20130620), sodium hydroxide (Beijing Chemical Plant, batch number: 20130520), calcium lactate (Tianjin Guangfu Fine Chemical Research Institute, batch number: 2013.1.23)

[0195] 4. Experimental methods:

[0196] The test substance was orally administered to mice for 31 days, with a daily dose of 100 mL / 60 kg BW.

[0197] 4.1 Weighted swimming test:

[0198] 30 minutes after the last administration of the test substance to the mice, the mice were placed in a swimming box with a lead wire of 5% of their body weight at the base of their tails. The water depth was 30 cm and the water temperature was 25.0℃±1.0℃. The time from the start of swimming to the death of the mice was recorded as the weighted swimming time of the mice.

[0199] Swimming time is a quantitative data. If the swimming time of the test substance group is significantly longer than that of the blank control group, and the difference is significant (P<0.05), it can be determined that the test substance has the effect of prolonging the weight-bearing swimming time of mice.

[0200] 4.2 Serum urea determination:

[0201] Thirty minutes after the last administration of the test substance to the mice, they were made to swim in water at 30 °C for 90 minutes. After a 60-minute rest, 0.5 mL of blood was collected by puncturing the eye socket immediately. The blood sample was placed in a refrigerator at 4 °C for about 3 hours. After the blood coagulated, it was centrifuged at 3000 r / min for 15 minutes, and the serum was taken for determination on a VITALAB SELECTRA-E type biochemical analyzer.

[0202] The obtained data were measurement data. If the serum urea content in the test substance group was significantly lower than that in the blank control group, and the difference was significant (P < 0.05), it could be determined that the test substance had the effect of reducing the urea content in fatigued mice.

[0203] 4.3 Determination of liver glycogen: Anthrone method

[0204] Thirty minutes after the last administration of the test substance to the mice, they were sacrificed immediately. The livers were taken out, rinsed with physiological saline, and then dried with filter paper. Exactly 100 mg of the liver was weighed and added to 4.0 mL of TCA, and homogenized for 1 minute. The homogenate was poured into a centrifuge tube and centrifuged at 3000 r / min for 15 minutes. 0.5 mL of the supernatant was taken, 2 mL of 95% ethanol was added, and the mixture was thoroughly mixed until there was no interface between the two liquids. It was stoppered with a clean stopper and left standing upright at room temperature overnight. The next day, it was centrifuged at 3000 r / min for 15 minutes. The supernatant was carefully poured out and the test tube was placed upside down for 10 minutes. The glycogen was dissolved with 2.00 mL of distilled water for determination. 0.25 mL of the test solution and 0.25 mL of distilled water were put into a test tube and mixed. Reagent blank tube: Pipette 0.5 mL of distilled water into a clean test tube. Standard tube: Pipette 0.125 mL of glucose standard solution (50 mg / dL) and 0.375 mL of distilled water into a test tube and mix.

[0205] 2.5 mL of anthrone reagent was vigorously added to each tube, boiled for 15 minutes, then transferred to an ice-water bath. After cooling, the absorbance was measured at a wavelength of 620 nm after zeroing with the reagent blank tube. The liver glycogen content was calculated according to the following formula:

[0206]

[0207] DU: Absorbance of the sample tube V: Volume of the extraction solution (ml); 16: Dilution factor DS: Absorbance of the standard tube 0.0625: Glucose content in 0.125 ml of glucose standard solution G: Weight of liver tissue (g) 0.9: Coefficient for converting glucose to glycogen

[0208] The obtained data were measurement data. If the liver glycogen content in the test substance group was significantly higher than that in the blank control group, and the difference was significant (P < 0.05), it could be determined that the test substance had the effect of promoting liver glycogen storage in mice.

[0209] 4.4 Determination of blood lactic acid:

[0210] Establishment of high blood lactate model and preparation of blood specimens: 30 minutes after the last administration of the test substance, before swimming, 20 μL of blood was taken from the medial canthus venous plexus of the mouse and added to 0.48 mL of 1% NaF solution, and then 1.5 mL of protein precipitant was added, and the mixture was shaken well; then the mouse swam in water at 30 °C without load for 10 minutes and then stopped, and immediately 20 μL of blood was taken and added to 0.48 mL of 1% NaF solution, and then 1.5 mL of protein precipitant was added, and the mixture was shaken well; after resting for 20 minutes, immediately 20 μL of blood was taken and added to 0.48 mL of 1% NaF solution, and then 1.5 mL of protein precipitant was added, and the mixture was shaken well. All were centrifuged at 3000 r / min for 10 minutes, and the supernatant was taken and operated according to the following table.

[0211] Determination of blood lactate in samples:

[0212]

[0213] After the above steps were completed, the mixture was shaken well, placed in a water bath at 30 °C for 30 minutes (shaken once every 10 minutes). After taking it out, it was heated in a boiling water bath for 90 s, taken out and cooled to room temperature, and colorimetric analysis was performed at a wavelength of 560 nm using a colorimetric cuvette with a 5 mm optical path, and the blank tube was zeroed.

[0214] Calculate the blood lactate content and the area under the blood lactate curve according to the following calculation formula:

[0215]

[0216] Area under the blood lactate curve = 5 × (blood lactate value before swimming + 3 × blood lactate value at 0 min after swimming + 2 × blood lactate value at 20 min after rest after swimming)

[0217] The obtained data are measurement data. If the area under the blood lactate curve of the test substance group is significantly lower than that of the blank control group and the difference is significant (P < 0.05), it can be determined that the test substance has the effect of reducing the area under the blood lactate curve in mice after swimming.

[0218] 4.5 Statistical analysis of experimental data: Data processing was performed using SPSS software. Analysis of variance was used, but the homogeneity of variance test was first performed according to the procedure of analysis of variance. If the variances were homogeneous, the F value was calculated. If the F value < F0.05, the conclusion was that there was no significant difference among the means of each group; if the F value ≥ F0.05 and P ≤ 0.05, pairwise comparison of the means between multiple experimental groups and one control group was used for statistics; for non-normal or heterogeneous variance data, appropriate variable transformation was performed, and after meeting the requirements of normality or homogeneity of variance, the transformed data were used for statistics; if the purpose of normality or homogeneity of variance was still not achieved after variable transformation, the rank sum test was used for statistics.

[0219] II. Test results

[0220] 1. Effect of Cistanche deserticola health care wine on the weight-bearing swimming time of mice

[0221]

[0222]

[0223] Note: * indicates P < 0.05, ** indicates P < 0.01, the same below.

[0224] 2. Effect of Cistanche deserticola health care wine on the serum urea level of mice after swimming

[0225] Group Number of animals (pcs) Serum urea (mmol / L) The first group 12 11.5±1.8 The second group 12 11.3±1.3 The third group 12 10.8±1.6* The fourth group 12 11.0±1.7* The fifth group 12 11.1±1.7 The sixth group 12 13.3±1.7 The seventh group 12 11.4±1.5 The eighth group 12 11.7±1.3 The ninth group 12 11.5±1.5 The tenth group 12 12.1±1.7 The eleventh group 12 12.5±2.1 The twelfth group 12 12.7±1.9

[0226] 3. Effect of Cistanche deserticola health care wine on the liver glycogen content of mice

[0227]

[0228]

[0229] 4. Effect of Cistanche deserticola wine on the blood lactic acid value of mice after swimming

[0230]

[0231]

[0232] III. Summary

[0233] According to the above results, it can be seen that: compared with the blank control group, the sample group can extend the weight-bearing swimming time of mice, reduce the serum urea content of fatigued mice, increase the liver glycogen content of mice, and reduce the area under the blood lactic acid curve of mice after swimming. According to the judgment criteria for health foods for relieving physical fatigue in the "Technical Specifications for Inspection and Evaluation of Health Foods" (2025 Edition), the Cistanche deserticola health care wine provided by the present invention has the function of relieving physical fatigue. And the anti-fatigue effect of the third group is the best, superior to other groups, indicating that the raw material ratio and preparation process of Example 4 are reasonable and present a synergistic effect.

[0234] Obviously, the above examples are only for clear illustration and not a limitation of the implementation manner. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A Cistanche health wine, characterized in that: Contains the following active ingredients: Phenylethanol glycosides 40mg / 100g-110mg / 100g; and Iridoid terpenes 4mg / 100g-15mg / 100g.

2. The Cistanche deserticola health wine according to claim 1, characterized in that: The Cistanche health wine also contains the following active ingredients: Total saponins 16mg / 100g-42mg / 100g; Total flavonoids 320mg / 100g-830mg / 100g; and Crude polysaccharides 26mg / 100g-42mg / 100g.

3. The Cistanche deserticola health wine according to claim 1 or 2, characterized in that: The raw material composition includes, by weight: Cistanche deserticola 6-30 servings 10-30 servings of American ginseng Cordyceps militaris 5-20 portions 10-20 servings of Pueraria root 100-260 servings of wine.

4. The Cistanche deserticola health wine according to claim 3, characterized in that: The wine is white wine with an alcohol content of 30-80 degrees.

5. The Cistanche deserticola health wine according to claim 4, characterized in that: The liquor is 190-250 portions; the alcohol content of the liquor is 65 degrees.

6. The Cistanche deserticola health wine according to claim 4 or 5, characterized in that: The raw material composition includes, by weight:

7. A method for preparing the Cistanche deserticola health wine according to any one of claims 1 to 6, characterized in that: The following steps are involved: Pre-treating the Cistanche deserticola under the conditions of 3-7 atmospheres, 60-70°C, and 24.2-37% humidity, then soaking it in wine, and stirring it once at intervals to obtain Cistanche deserticola wine; Crush the American ginseng, then add wine to soak it, and stir it once at intervals to obtain American ginseng wine; Crush the Cordyceps militaris, then add wine to soak, and stir once at intervals to obtain Cordyceps militaris wine; Crush the kudzu root, then add wine to soak, stirring once at intervals to obtain kudzu root wine; The product is prepared by mixing Cistanche deserticola liquor, American ginseng liquor, Cordyceps militaris liquor and Pueraria root liquor.

8. The method for preparing the Cistanche deserticola health wine according to claim 7, characterized in that: Cordyceps militaris and / or Pueraria root must be steamed for at least 30 minutes before crushing; and / or, The first period of time is at least 30 days; and / or, The stirring time is at least 30 minutes.

9. The method for preparing the Cistanche deserticola health wine according to claim 7 or 8, characterized in that: The mass ratios of the Cistanche deserticola, American ginseng, Cordyceps militaris, Pueraria lobata and wine are 1:(2-30) respectively.

10. Use of the Cistanche deserticola health wine according to any one of claims 1 to 6 or the Cistanche deserticola health wine prepared by the preparation method according to any one of claims 7 to 9 in relieving physical fatigue.

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