Cell culture device and method
By designing a cell culture device including limiting, slanting and oscillating mechanisms, the problem of insufficient mixing of cells and suspensions in the prior art is solved, more efficient cell culture is achieved, and the culture container is protected by flexible contact material.
Patent Information
- Application Number
- CN202510060221.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-15
- Publication Date
- 2025-05-30
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
It is difficult for existing cell culture devices to fully mix cells with the suspension, resulting in poor culture effect, and the hard contact between the limiting assembly and the culture container is easily damaged during violent shock.
A cell culture device including a base, a limiting mechanism, a slanting mechanism and an oscillating mechanism is designed. Through a motor-driven synchronous belt and threaded rod system, a variety of forms of container mixing, including centrifugal motion, slanting mixing and oscillating mixing.
Full mixing of cells and suspension is achieved, the culture effect is improved, and damage to the culture container is reduced by flexible contact with the material.
Smart Images
Figure CN120059941A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of cell culture, and specifically provides a cell culture device and method. Background Technique
[0002] Cell culture technology is an important part of biotechnology and plays an invaluable role in life science research. Currently, in the process of small-scale cell suspension culture, the cell suspension is placed into a shaking flask, and then a shaker is used for shaking culture, so that nutrients can come into uniform contact with the cells, and then the cells are cultured.
[0003] For example, in a cell culture device with the publication number CN118620728A, when clamping shaking flasks of different specifications and sizes, by sliding the limiting plate, the size of the clamping gap can be changed to adapt to shaking flasks of different sizes, which helps to improve the applicability of the shaker. And under the action of the compression spring, the limiting plate can be pressed tightly against the shaking flask to improve the stability of the shaking flask. Then, the driving device is started to drive the shaker to generate vibration to mix the cells and the suspension in the shaking flask.
[0004] When the cell culture device in the above solution is culturing cells, the culture container needs to be placed into the culture device, and then the cell culture device is started to make the driving components inside generate vibration to mix the cells and the suspension in the culture container. When the existing cell culture device mixes the suspension and the cells, it can only drive the culture container to generate vibration through the internal driving device to mix them. The mixing form is single, and it is difficult to perform centrifugal mixing and pendulum mixing. It is difficult for the cells and the suspension to come into uniform contact, and the suspension and the cells in the culture container are prone to upper and lower stratification. Moreover, when the existing cell culture device fixes the culture container, the limiting component and the outer wall of the culture container are in hard contact, which is easy to damage the container during severe shaking. Summary of the Invention
[0005] The purpose of the present invention is to provide a cell culture device and method to solve the problem that the existing cell culture device in the above background technique is difficult to fully mix the cells and the suspension.
[0006] To achieve the above purpose, the present invention provides the following technical solution: A cell culture device includes a base and a limiting mechanism. A vertical rod is fixed to the top of the base, and a top plate is fixed to the top of the vertical rod.
[0007] A motor a is installed inside the base. A synchronous pulley a is fixed to the output end of the motor a. A synchronous belt is sleeved outside the synchronous pulley a. A synchronous pulley b is sleeved on one side of the synchronous belt. Threaded rods a are fixedly connected to the tops of both the synchronous pulley b and the synchronous pulley a. A moving disk is threadedly connected to the outside of the threaded rod a. A toothed ring is fixedly connected to the top of the moving disk. A limiting ring is slidably connected inside the moving disk. Multiple groups of balls a are rollingly connected to the bottom, outside, and inside of the limiting ring. A connecting disk is fixedly connected to the top of the limiting ring. A connecting shaft is rotatably connected to the inside of the connecting disk through a bearing. A rectangular rod is slidably connected to the inside of the connecting shaft. Multiple groups of balls b are rollingly connected to the outside of the rectangular rod, and the multiple groups of balls b are evenly distributed on the rectangular rod. A limiting mechanism for limiting the container is provided at the top of the rectangular rod. The limiting mechanism includes a placing cylinder fixedly connected to the top of the rectangular rod. A limiting plate is rotatably connected to the inside of the placing cylinder through a rotating shaft. One side of the limiting plate is fixedly connected to a telescopic spring a. Multiple groups of supporting springs are fixedly connected to the top of the placing cylinder. A ball c is rollingly connected to the bottom of the rectangular rod. A convex block is fixedly connected to the inside of the moving disk. One end of the convex block is hemispherical. A fixing rod is provided at one end of the convex block. A spiral groove is formed on the outside of the fixing rod, and the spiral groove is slidably connected to the convex block. A gear body is fixedly connected to the outside of the placing cylinder. A support frame is fixedly connected to the top of the moving disk. A shifting rod is fixedly connected to one side of the top of the support frame. A yawing mechanism for driving the placing cylinder to yaw is provided at the bottom of the top plate. The yawing mechanism includes a connecting seat rotatably connected to the top of the top plate through a rotating shaft. A moving rod is slidably connected to the inside of the connecting seat. One end of the moving rod is rotatably connected to a connecting rod through a rotating shaft. One end of the connecting rod is rotatably connected to a moving plate through a rotating shaft. A blocking rod is fixedly connected to the bottom of the moving plate. An oscillating mechanism for driving the placing cylinder to vibrate is provided inside the base. The oscillating mechanism includes a motor b fixedly connected to the inside of the base. A disk a is fixedly connected to the output end of the motor b. An electric push rod is fixed to the top of the disk a. A disk b is fixed to the top of the electric push rod. A rubber sleeve is provided on the top of the disk b.
[0008] Preferably, the top of the threaded rod a is rotatably connected to the top plate through a bearing. The multiple groups of balls a are distributed at equal angles with respect to the central axis of the limiting ring. The outside of the ball b is rollingly connected to the connecting shaft. The top of the fixing rod is fixedly connected to the top plate. The outside of the gear body is meshed with the toothed ring.
[0009] Preferably, multiple groups of the limiting plates are provided inside the placing cylinder, and the multiple groups of limiting plates are distributed at equal angles with respect to the central axis of the placing cylinder. One end of the telescopic spring a is fixedly connected to the inside of the placing cylinder. Multiple groups of the supporting springs are provided on the top of the placing cylinder, and the multiple groups of supporting springs are distributed at equal angles with respect to the central axis of the placing cylinder.
[0010] Preferably, a plastic ring is fixedly connected to the top of the support spring, a rubber ring is fixedly connected to the inner side of the plastic ring, a plurality of rollers are movably connected to the outer side of the plastic ring through a rotating shaft, and the plurality of rollers are distributed at equal angles with respect to the central axis of the plastic ring. A supporting seat is fixedly connected to the bottom of the placing cylinder, the outer shape of the supporting seat is semicircular, and a spherical groove is formed at the center.
[0011] Preferably, a sliding rod is slidably connected to the inside of the moving plate, a telescopic spring b is sleeved on the outer side of the sliding rod, one end of the telescopic spring b abuts against the moving plate, the other end of the telescopic spring b abuts against the top plate, both ends of the sliding rod are fixedly connected to the top plate, the outer side of the moving plate is slidably connected to the top plate, and an arc-shaped plate is fixedly connected to the bottom of the connecting seat.
[0012] Preferably, a straight groove a is formed at the bottom of the arc-shaped plate, one end of the straight groove a is provided with an inclined groove, one end of the inclined groove is connected to a straight groove b, both the inclined groove and the straight groove b are formed inside the arc-shaped plate, a reset spring is fixedly connected to one side of the arc-shaped plate, one end of the reset spring is fixedly connected to the top plate, and the outer side of the arc-shaped plate is slidably connected to the inside of the top plate.
[0013] Preferably, a handle is movably connected to the top of the connecting seat through a bearing, a bevel gear a is fixedly connected to the bottom of the handle, a bevel gear b is meshed and connected to the outer side of the bevel gear a, a threaded rod b is fixedly connected to one side of the bevel gear b, one end of the threaded rod b is movably connected to the connecting seat through a bearing, and the other end of the threaded rod b is threadedly connected to the moving rod.
[0014] Preferably, two sides of the bottom of the rubber sleeve are fixedly connected with support plates a, a ring is fixedly connected to the bottom of the rubber sleeve, the bottom of the support plates a is fixedly connected to the ring, the center of the bottom of the rubber sleeve abuts against a support plate b, a plug rod is fixedly connected to the top of the support plate b, the outer side of the plug rod is inserted into the rubber sleeve, and a connecting rod a is fixedly connected to the bottom of the support plate b.
[0015] Preferably, the outer side of the connecting rod a is slidably connected to the ring, a connecting rod b is fixedly connected to the bottom of the ring, the bottom of the connecting rod b is fixedly connected to the disc a, the bottom of the connecting rod a is fixedly connected to the disc b, a plurality of groups of the rubber sleeves are arranged on the top of the disc b, and the plurality of groups of disc b are distributed at equal angles with respect to the central axis of the disc b. The outer shapes of the support plates a and the support plates b are both arc-shaped.
[0016] A cell culture method includes the following steps:
[0017] S1. Clean the container, and then add the suspension and the cell sample into the container;
[0018] S2. Feed the container filled with the suspension and the cell sample into the limiting mechanism of the culture device;
[0019] S3. Start motor a to drive the limiting mechanism and the container inside it to perform various forms of mixing, so that the cells and the suspension inside the container are fully mixed, accelerating the cell culture;
[0020] S4. Take out the container with the mixing completed from the culture device.
[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: For this cell culture device and method, when culturing cells, by placing the container filled with cells and suspension into the limiting mechanism, and then starting motor a to drive the moving disk to move upward, the connecting disk will contact the spiral groove on the outer side of the fixed rod through the bumps inside, causing the connecting disk to rotate when moving upward. At this time, the container in the placing cylinder will form two forms of centrifugal motion, thereby making the suspension and cells inside the container fully mixed. As the moving disk continues to move upward, the limiting mechanism will trigger the yaw mechanism, so that the suspension and cells inside it perform yaw mixing, accelerating the mixing of the two. When the moving disk moves to the lowest point, it will trigger the oscillation mechanism, causing the cells and suspension inside the container to generate oscillating mixing, further improving the mixing effect. In this way, through the above operations, the cells and suspension in the container of the culture device can be mixed alternately in various forms, improving the mixing uniformity, and thus enhancing the cell culture effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a three-dimensional sectional view of the present invention;
[0023] Figure 2 It is a three-dimensional view of the present invention;
[0024] Figure 3 It is a three-dimensional front view of the base of the present invention;
[0025] Figure 4 It is a three-dimensional view of the placing cylinder of the present invention;
[0026] Figure 5 It is a three-dimensional sectional view of the placing cylinder of the present invention;
[0027] Figure 6 It is a three-dimensional view of the top plate of the present invention;
[0028] Figure 7 It is a three-dimensional sectional view of the top plate of the present invention;
[0029] Figure 8 It is an enlarged view A of the present invention;
[0030] Figure 9 It is an enlarged view B of the present invention;
[0031] Figure 10 Top view schematic diagram of the top plate of the present invention;
[0032] Figure 11 Stereoscopic schematic diagram of the connecting shaft of the present invention;
[0033] Figure 12 Stereoscopic schematic diagram of the rubber sleeve of the present invention;
[0034] Figure 13 Stereoscopic sectional schematic diagram of the rubber sleeve of the present invention.
[0035] In the figure: 1, base; 2, vertical rod; 3, top plate; 4, motor a; 5, synchronous pulley a; 6, synchronous belt; 7, synchronous pulley b; 8, threaded rod a; 9, moving plate; 10, gear ring; 11, limiting ring; 12, ball a; 13, connecting plate; 14, connecting shaft; 15, rectangular rod; 16, ball b; 17, limiting mechanism; 171, placing cylinder; 172, limiting plate; 173, telescopic spring a; 174, supporting spring; 175, plastic ring; 176, rubber ring; 177, roller; 178, supporting seat; 18, ball c; 19, convex block; 20, fixed rod; 21, spiral groove; 22, gear body; 23, support frame; 24, lever; 25, yaw mechanism; 251, connecting seat; 252, moving rod; 253, connecting rod; 254, moving plate; 255, blocking rod; 256, sliding rod; 257, telescopic spring b; 258, arc plate; 259, straight groove a; 2510, inclined groove; 2511, straight groove b; 2512, reset spring; 2513, handle; 2514, bevel gear a; 2515, bevel gear b; 2516, threaded rod b; 26, oscillation mechanism; 261, motor b; 262, disc a; 263, electric push rod; 264, disc b; 265, rubber sleeve; 266, support plate a; 267, insertion rod; 268, support plate b; 269, connecting rod a; 2610, ring; 2611, connecting rod b. Detailed implementation manners
[0036] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0037] Please refer to Figures 1-13 , the present invention provides a technical solution: a cell culture device, including a base 1 and a limiting mechanism 17. A vertical rod 2 is fixed on the top of the base 1, and a top plate 3 is fixed on the top of the vertical rod 2,
[0038] Inside the base 1, a motor a4 is installed. The output end of the motor a4 is fixed with a synchronous pulley a5. A synchronous belt 6 is sleeved outside the synchronous pulley a5. One side of the synchronous belt 6 is sleeved with a synchronous pulley b7. Both the top of the synchronous pulley b7 and the synchronous pulley a5 are fixedly connected with a threaded rod a8. A moving disk 9 is threadedly connected to the outside of the threaded rod a8. The top of the moving disk 9 is fixedly connected with a gear ring 10. Inside the moving disk 9, a limiting ring 11 is slidably connected. Multiple groups of ball a12 are rollingly connected to the bottom, outside and inside of the limiting ring 11. The top of the limiting ring 11 is fixedly connected with a connecting disk 13. Inside the connecting disk 13, a connecting shaft 14 is rotatably connected through a bearing. Inside the connecting shaft 14, a rectangular rod 15 is slidably connected. Multiple groups of ball b16 are rollingly connected to the outside of the rectangular rod 15, and the multiple groups of ball b16 are equally spaced on the rectangular rod 15. At the top of the rectangular rod 15, a limiting mechanism 17 for limiting the container is provided;
[0039] The limiting mechanism 17 includes a placing cylinder 171 fixedly connected to the top of the rectangular rod 15. Inside the placing cylinder 171, a limiting plate 172 is rotatably connected through a rotating shaft. One side of the limiting plate 172 is fixedly connected with a telescopic spring a173. Multiple groups of support springs 174 are fixedly connected to the top of the placing cylinder 171. A ball c18 is rollingly connected to the bottom of the rectangular rod 15. A convex block 19 is fixedly connected to the inside of the moving disk 9. One end of the convex block 19 is hemispherical. A fixing rod 20 is provided at one end of the convex block 19. A spiral groove 21 is opened on the outside of the fixing rod 20, and the spiral groove 21 is slidably connected with the convex block 19. A gear body 22 is fixedly connected to the outside of the placing cylinder 171. A support frame 23 is fixedly connected to the top of the moving disk 9. One side of the top of the support frame 23 is fixedly connected with a lever 24. At the bottom of the top plate 3, a yawing mechanism 25 for driving the placing cylinder 171 to yaw is provided;
[0040] The yawing mechanism 25 includes a connecting seat 251 rotatably connected to the top of the top plate 3 through a rotating shaft. Inside the connecting seat 251, a moving rod 252 is slidably connected. One end of the moving rod 252 is rotatably connected with a connecting rod 253 through a rotating shaft. One end of the connecting rod 253 is rotatably connected with a moving plate 254 through a rotating shaft. A stop rod 255 is fixedly connected to the bottom of the moving plate 254. Inside the base 1, an oscillating mechanism 26 for driving the placing cylinder 171 to vibrate is provided. The oscillating mechanism 26 includes a motor b261 fixedly connected to the inside of the base 1. The output end of the motor b261 is fixedly connected with a disk a262. A power-assisted push rod 263 is fixed on the top of the disk a262. A disk b264 is fixed on the top of the power-assisted push rod 263. A rubber sleeve 265 is provided on the top of the disk b264;
[0041] The top of the threaded rod a8 is movably connected to the top plate 3 through a bearing. Multiple groups of balls a12 are distributed at equal angles with respect to the central axis of the limit ring 11. The outer side of the ball b16 is in rolling connection with the connecting shaft 14. The top of the fixed rod 20 is fixedly connected to the top plate 3. The outer side of the gear body 22 is in meshing connection with the gear ring 10. The gear body 22 and the gear ring 10 form a meshing transmission structure. The threaded rod a8 and the moving plate 9 form a threaded transmission structure. An annular groove matching the limit ring 11 is opened inside the moving plate 9. The limit ring 11 and the moving plate 9 form a sliding structure; Multiple groups of limit plates 172 are arranged inside the placing cylinder 171, and the multiple groups of limit plates 172 are distributed at equal angles with respect to the central axis of the placing cylinder 171. One end of the telescopic spring a173 is fixedly connected to the inner side of the placing cylinder 171. Multiple groups of support springs 174 are arranged on the top of the placing cylinder 171, and the multiple groups of support springs 174 are distributed at equal angles with respect to the central axis of the placing cylinder 171;
[0042] The top of the support spring 174 is fixedly connected with a plastic ring 175. The inner side of the plastic ring 175 is fixedly connected with a rubber ring 176. The outer side of the plastic ring 175 is movably connected with multiple groups of rollers 177 through a rotating shaft, and the multiple groups of rollers 177 are distributed at equal angles with respect to the central axis of the plastic ring 175. The bottom of the placing cylinder 171 is fixedly connected with a supporting seat 178. The shape of the supporting seat 178 is semi-circular, and a spherical groove is opened at the center. The limit plate 172 is rotationally connected to the placing cylinder 171 through a rotating shaft; A sliding rod 256 is slidably connected inside the moving plate 254. A telescopic spring b257 is sleeved on the outer side of the sliding rod 256. One end of the telescopic spring b257 abuts against the moving plate 254, and the other end of the telescopic spring b257 abuts against the top plate 3. Both ends of the sliding rod 256 are fixedly connected to the top plate 3. The outer side of the moving plate 254 is slidably connected to the top plate 3. The bottom of the connecting seat 251 is fixedly connected with an arc-shaped plate 258.; A straight groove a259 is opened at the bottom of the arc-shaped plate 258. One end of the straight groove a259 is provided with an inclined groove 2510. One end of the inclined groove 2510 is connected with a straight groove b2511. Both the inclined groove 2510 and the straight groove b2511 are opened inside the arc-shaped plate 258. One side of the arc-shaped plate 258 is fixedly connected with a return spring 2512. One end of the return spring 2512 is fixedly connected to the top plate 3. The outer side of the arc-shaped plate 258 is slidably connected inside the top plate 3;
[0043] The top of the connecting seat 251 is movably connected with a handle 2513 through a bearing. The bottom of the handle 2513 is fixedly connected with a bevel gear a 2514. The outside of the bevel gear a 2514 is meshed with a bevel gear b 2515. One side of the bevel gear b 2515 is fixedly connected with a threaded rod b 2516. One end of the threaded rod b 2516 is movably connected with the connecting seat 251 through a bearing. The other end of the threaded rod b 2516 is threadedly connected with the moving rod 252. The threaded rod b 2516 and the moving rod 252 form a threaded transmission structure. A moving groove matching the moving rod 252 is opened inside the connecting seat 251. The moving rod 252 and the connecting seat 251 form a sliding structure. The bevel gear a 2514 and the bevel gear b 2515 form a meshing transmission structure. An arc-shaped groove matching the arc-shaped plate 258 is opened at the top of the top plate 3; both sides of the bottom of the rubber sleeve 265 are fixedly connected with a support plate a 266. The bottom of the rubber sleeve 265 is fixedly connected with a ring 2610. The bottom of the support plate a 266 is fixedly connected with the ring 2610. The center of the bottom of the rubber sleeve 265 abuts against a support plate b 268. The top of the support plate b 268 is fixedly connected with an insertion rod 267. The outside of the insertion rod 267 is inserted into the rubber sleeve 265. The bottom of the support plate b 268 is fixedly connected with a connecting rod a 269; the outside of the connecting rod a 269 is slidably connected with the ring 2610. The bottom of the ring 2610 is fixedly connected with a connecting rod b 2611. The bottom of the connecting rod b 2611 is fixedly connected with a disc a 262. The bottom of the connecting rod a 269 is fixedly connected with a disc b 264. Multiple groups of the rubber sleeve 265 are arranged on the top of the disc b 264, and the multiple groups of the disc b 264 are distributed at equal angles about the central axis of the disc b 264. The outer shapes of the support plate a 266 and the support plate b 268 are both arc-shaped. A through hole matching the insertion rod 267 is opened inside the rubber sleeve 265. A sliding groove matching the connecting rod a 269 is opened inside the ring 2610. The connecting rod a 269 and the ring 2610 form a sliding structure.
[0044] In specific implementation, for the cell culture device and method, when culturing cells, by placing the container filled with cells and suspension into the placement cylinder 171 of the limiting mechanism 17, the container will pass through the plastic ring 175 and the rubber ring 176 and enter the placement cylinder 171, and contact with multiple groups of limiting plates 172 inside the placement cylinder 171, and be squeezed by them. The limiting plates 172 will squeeze the telescopic spring a 173 on one side, and the restoring force generated by the telescopic spring a 173 will push the limiting plates 172 to clamp and limit the container. The bottom of the container will contact the support seat 178 made of sponge material. After the container is installed, the motor a 4 can be started. The motor a 4 is a servo motor and will rotate forward and backward alternately. When the motor a 4 rotates forward, it will drive the threaded rod a 8 and the moving disk 9 to perform threaded transmission, causing the moving disk 9 to move upward. When the moving disk 9 moves, the bump 19 inside the connecting disk 13 will contact the spiral groove 21 on the outer side of the fixed rod 20, causing the connecting disk 13 to rotate when moving upward. The limiting ring 11 at the bottom of the connecting disk 13 will rotate inside the moving disk 9 and contact the moving disk 9 through the ball a 12 to reduce the friction during rotation. When the connecting disk 13 rotates, it can drive multiple groups of placement cylinders 171 at the top to rotate through the connecting shaft 14 and the rectangular rod 15, causing the gear body 22 on the outer side of the placement cylinder 171 to mesh with the toothed ring 10. At this time, the placement cylinder 171 will not only rotate following the toothed ring 10 but also rotate self - sufficiently, forming two forms of centrifugal motion, thereby enabling the suspension and cells inside the container to be fully mixed;
[0045] As the moving plate 9 continues to move upward, when the limiting mechanism 17 is about to move to one side of the blocking rod 255, the lever 24 on the top of the moving plate 9 will first contact the straight groove a259 at the bottom of the arc plate 258, and enter the inclined groove 2510 under the guidance of the straight groove a259, thereby shifting the arc plate 258 and the connecting seat 251 on the top to rotate. When the arc plate 258 rotates, it squeezes the return spring 2512 on one side, and when the connecting seat 251 rotates, it pulls the multiple connecting rods 253 to rotate through the moving rod 252, thereby causing the connecting rod 253 to rotate. The movable plate 254 at one end of the movable plate 253 slides on the outside of the slide rod 256, and the telescopic spring b257 on one side of the movable plate 254 will be squeezed when it slides, and the blocking rod 255 at the bottom of the movable plate 254 will also move accordingly. When the lever 24 moves into the straight groove b2511, the arc plate 258 will complete the rotation and drive the blocking rod 255 to contact the rotating limit mechanism 17. Since the blocking rod 255 is movable, it can prevent the limit mechanism 17 from directly colliding with the bottom of the blocking rod 255 when moving up, causing damage to both. The positioning mechanism 17 will come into contact with the blocking rod 255 through the roller 177, so that the plastic ring 175 moves. When the plastic ring 175 moves, the multiple groups of supporting springs 174 at the bottom will be deformed, and the rubber ring 176 inside the plastic ring 175 will drive the container to swing, and the outer side of the container will contact the multiple groups of limiting plates 172. The multiple groups of limiting plates 172 will squeeze the telescopic spring a173 on one side to adapt to the swing of the container. The elastic force generated by the supporting spring 174 will drive the rubber ring 176 to drive the container to produce continuous non-stop The movable plate 258 is swung regularly, so that the suspension inside it is mixed with the cells by eccentric swing, which accelerates the mixing of the two and improves the mixing efficiency. When the gear ring 10 moves to the top, the motor a4 will reverse, thereby driving the movable plate 9 to move downward. The movable plate 9 will also reverse when moving, so that the container can be centrifugally mixed in another direction. When the movable plate 9 drives the lever 24 to leave the interior of the arc plate 258, the reset spring 2512 will push the connecting seat 251 to reset, so that the straight groove a259 at the bottom of the arc plate 258 is always facing the lever 24.
[0046] When the deflection amplitude of the deflection mechanism 25 needs to be adjusted, the handle 2513 can be turned to drive the bevel gear a2514 to mesh with the bevel gear b2515, and the bevel gear b2515 will drive the threaded rod b2516 on one side to perform threaded transmission with the moving rod 252, so that the moving rod 252 pulls the moving plate 254 to move through the connecting rod 253, and the moving plate 254 will drive the blocking rod 255 to move to a distance closer to the axis of the connecting seat 251. In this way, when the deflection mechanism 25 is operated next time, the blocking rod 255 can be closer to the limiting mechanism 17, and the limiting mechanism 17 can be moved to a larger amplitude, so that the deflection amplitude of the container in the limiting mechanism 17 is increased, thereby adapting to different usage requirements;
[0047] When the moving disk 9 moves to the lowest point, the bottom of the rectangular rod 15 will contact multiple sets of rubber sleeves 265 in the oscillation mechanism 26 through the ball c18. The motor a4 will stop rotating for a period of time, while the motor b261 will start and drive the ring 2610 to rotate through the disk a262 and the connecting rod b2611. Multiple sets of raised rubber sleeves 265 on the top of the ring 2610 will contact the ball c18 at the bottom of the rectangular rod 15, causing the rectangular rod 15 to move up and down in the connecting shaft 14. The placement cylinder 171 at the top of the rectangular rod 15 will also move up and down, thereby causing the internal container to produce an oscillating effect, making the cells and the suspension in the container oscillate and mix, further improving the mixing effect. Moreover, the oscillation mechanism 26 can be adjusted. When it is necessary to reduce the oscillation amplitude and increase the oscillation frequency, the electric push rod 263 is started to pull the disk b264 downward. The disk b264 will pull the support plate b268 and the insertion rod 267 downward through the connecting rod a269. The insertion rod 267 will then pull the rubber sleeve 265 downward, causing a depression in the middle of the rubber sleeve 265. At this time, when the rubber sleeve 265 contacts the rectangular rod 15, the distance that the rectangular rod 15 moves up and down will decrease. However, since the middle of the rubber sleeve 265 is in a depressed state, the frequency of the rectangular rod 15 moving up and down will increase, thereby changing the oscillation state of the container in the limiting mechanism 17 to adapt to different mixing requirements. After the motor a4 stops rotating for a period of time, it will rotate forward again, driving the moving disk 9 to perform up and down reciprocating movement again, so that the suspension and cells in the internal container are mixed again. In this way, through the above operations, the cells and the suspension in the container of the culture device can be mixed alternately in various forms, improving the mixing uniformity and thus enhancing the cell culture effect.
[0048] A cell culture method includes the following steps:
[0049] S1. Clean the container, and then add the suspension and the cell sample into the container;
[0050] S2. Send the container containing the suspension and the cell sample into the limiting mechanism 17 of the culture device;
[0051] S3. Start the motor a4 to drive the limiting mechanism 17 and the container inside it to perform various forms of mixing, so that the cells and the suspension inside the container are fully mixed, accelerating the cell culture;
[0052] S4. Take out the container with the mixing completed from the culture device.
[0053] In summary, by placing the container for culturing cells into the limiting mechanism 17 and then starting the motor a4 to drive the moving disk 9 to move up and down, the yaw mechanism 25 and the oscillation mechanism 26 are triggered, so as to fully mix the cells and the suspension in the container and accelerate the cell culture. The content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.
[0054] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.
Claims
1. A cell culture device, comprising a base (1) and a limiting mechanism (17), wherein a vertical rod (2) is fixed on the top of the base (1), and a top plate (3) is fixed on the top of the vertical rod (2), characterized in that: A motor a (4) is installed inside the base (1), a synchronous wheel a (5) is fixed to the output end of the motor a (4), a synchronous belt (6) is sleeved on the outer side of the synchronous wheel a (5), a synchronous wheel b (7) is sleeved on one side of the synchronous belt (6), a threaded rod a (8) is fixedly connected to the top of the synchronous wheel b (7) and the synchronous wheel a (5), a movable disk (9) is threadedly connected to the outer side of the threaded rod a (8), a gear ring (10) is fixedly connected to the top of the movable disk (9), a limit ring (11) is slidably connected to the inside of the movable disk (9), a plurality of groups of balls a (12) are rollingly connected to the bottom, outer side and inner side of the limit ring (11), and a connecting rod (10) is fixedly connected to the top of the limit ring (11). A connecting plate (13) is provided, wherein the interior of the connecting plate (13) is movably connected to a connecting shaft (14) via a bearing, the interior of the connecting shaft (14) is slidably connected to a rectangular rod (15), the outer side of the rectangular rod (15) is rollingly connected to multiple groups of balls b (16), and the multiple groups of balls b (16) are evenly distributed on the rectangular rod (15), the top of the rectangular rod (15) is provided with a limiting mechanism (17) for limiting the container, the limiting mechanism (17) comprises a placing cylinder (171) fixedly connected to the top of the rectangular rod (15), the interior of the placing cylinder (171) is movably connected to a limiting plate (172) via a rotating shaft, one side of the limiting plate (172) is fixedly connected to a telescopic spring a (173), the placing cylinder (171) is fixedly connected to the limiting plate (172), and the placing cylinder (171) is fixedly connected to the limiting plate (172). The top of the cylinder (171) is fixedly connected to a plurality of groups of supporting springs (174); the bottom of the rectangular rod (15) is rollingly connected to a ball c (18); the inner side of the movable plate (9) is fixedly connected to a protrusion (19); one end of the protrusion (19) is hemispherical; one end of the protrusion (19) is provided with a fixing rod (20); a spiral groove (21) is provided on the outer side of the fixing rod (20); and the spiral groove (21) is slidably connected to the protrusion (19); the outer side of the placement cylinder (171) is fixedly connected to a gear body (22); the top of the movable plate (9) is fixedly connected to a support frame (23); one side of the top of the support frame (23) is fixedly connected to a lever (24); the bottom of the top plate (3) is provided with A deflection mechanism (25) for driving the placement tube (171) to deflect, the deflection mechanism (25) comprising a connection seat (251) movably connected to the top of the top plate (3) via a rotating shaft, a moving rod (252) being slidably connected inside the connection seat (251), one end of the moving rod (252) being movably connected to a connecting rod (253) via a rotating shaft, one end of the connecting rod (253) being movably connected to a moving plate (254) via a rotating shaft, a blocking rod (255) being fixedly connected to the bottom of the moving plate (254), an oscillation mechanism (26) for driving the placement tube (171) to vibrate being arranged inside the base (1), the oscillation mechanism (26) comprising a motor b (261) fixedly connected to the inside of the base (1),The output end of the motor b (261) is fixedly connected to a disk a (262), an electric push rod (263) is fixed to the top of the disk a (262), a disk b (264) is fixed to the top of the electric push rod (263), and a rubber sleeve (265) is provided on the top of the disk b (264).
2. A cell culture device according to claim 1, characterized in that: The top of the threaded rod a (8) is movably connected to the top plate (3) via a bearing, a plurality of groups of balls a (12) are distributed at equal angles with respect to the central axis of the limiting ring (11), the outer side of the balls b (16) is rollingly connected to the connecting shaft (14), the top of the fixing rod (20) is fixedly connected to the top plate (3), and the outer side of the gear body (22) is meshingly connected to the gear ring (10).
3. A cell culture device according to claim 1, characterized in that: A plurality of groups of the limit plates (172) are arranged inside the placement tube (171), and the plurality of groups of limit plates (172) are distributed at equal angles with respect to the central axis of the placement tube (171); one end of the telescopic spring a (173) is fixedly connected to the inner side of the placement tube (171); a plurality of groups of the support springs (174) are arranged on the top of the placement tube (171), and the plurality of groups of support springs (174) are distributed at equal angles with respect to the central axis of the placement tube (171).
4. A cell culture device according to claim 1, characterized in that: The top of the support spring (174) is fixedly connected to a plastic ring (175), the inner side of the plastic ring (175) is fixedly connected to a rubber ring (176), the outer side of the plastic ring (175) is movably connected to a plurality of rollers (177) via a rotating shaft, and the plurality of rollers (177) are distributed at equal angles with respect to the central axis of the plastic ring (175), and the bottom of the placement cylinder (171) is fixedly connected to a supporting seat (178), the supporting seat (178) is semicircular in shape, and a spherical groove is provided at the center.
5. A cell culture device according to claim 1, characterized in that: The movable plate (254) is internally slidably connected to a slide rod (256), and the outer side of the slide rod (256) is sleeved with a telescopic spring b (257), one end of the telescopic spring b (257) is in contact with the movable plate (254), and the other end of the telescopic spring b (257) is in contact with the top plate (3), both ends of the slide rod (256) are fixedly connected to the top plate (3), the outer side of the movable plate (254) is slidably connected to the top plate (3), and the bottom of the connecting seat (251) is fixedly connected to an arc plate (258).
6. A cell culture device according to claim 5, characterized in that: A straight groove a (259) is provided at the bottom of the arc plate (258), one end of the straight groove a (259) is provided with an inclined groove (2510), one end of the inclined groove (2510) is connected to a straight groove b (2511), the inclined groove (2510) and the straight groove b (2511) are both provided inside the arc plate (258), one side of the arc plate (258) is fixedly connected with a return spring (2512), one end of the return spring (2512) is fixedly connected to the top plate (3), and the outer side of the arc plate (258) is slidably connected to the inside of the top plate (3).
7. A cell culture device according to claim 6, characterized in that: The top of the connecting seat (251) is movably connected to a handle (2513) via a bearing, the bottom of the handle (2513) is fixedly connected to a bevel gear a (2514), the outer side of the bevel gear a (2514) is meshingly connected to a bevel gear b (2515), one side of the bevel gear b (2515) is fixedly connected to a threaded rod b (2516), one end of the threaded rod b (2516) is movably connected to the connecting seat (251) via a bearing, and the other end of the threaded rod b (2516) is threadedly connected to the moving rod (252).
8. A cell culture device according to claim 1, characterized in that: Support plates a (266) are fixedly connected to both sides of the bottom of the rubber sleeve (265), a circular ring (2610) is fixedly connected to the bottom of the rubber sleeve (265), the bottom of the support plate a (266) is fixedly connected to the circular ring (2610), a support plate b (268) is abutted against the center of the bottom of the rubber sleeve (265), a plug rod (267) is fixedly connected to the top of the support plate b (268), the outer side of the plug rod (267) is plugged into the rubber sleeve (265), and a connecting rod a (269) is fixedly connected to the bottom of the support plate b (268).
9. A cell culture device according to claim 8, characterized in that: The outer side of the connecting rod a (269) is slidably connected to the circular ring (2610), the bottom of the circular ring (2610) is fixedly connected to the connecting rod b (2611), the bottom of the connecting rod b (2611) is fixedly connected to the disk a (262), the bottom of the connecting rod a (269) is fixedly connected to the disk b (264), the rubber sleeve (265) is arranged in multiple groups on the top of the disk b (264), and the multiple groups of disks b (264) are distributed at equal angles with respect to the central axis of the disk b (264), and the outer shapes of the support plate a (266) and the support plate b (268) are both arc-shaped.
10. A cell culture method, characterized in that: The following steps are involved: S1. Clean the container and then add the suspension and cell sample into the container; S2, placing the container containing the suspension and the cell sample into the limiting mechanism (17) of the culture device; S3, starting the motor a (4) to drive the limiting mechanism (17) and the container inside it to perform various forms of mixing, so that the cells inside the container are fully mixed with the suspension, thereby accelerating the cell culture; S4. Take the mixed container out of the culture device.