Fluorescence detection method and system for heavy metal ion concentration

Abstract

The invention relates to the technical field of heavy metal ion fluorescence detection, in particular to a fluorescence detection method and system for heavy metal ion concentration, and the method comprises the following steps: carrying out fluorescence intensity change test according to a probe concentration-volume ratio combination and an ion test gradient concentration sequence to obtain a fluorescence time sequence curve sequence; calculating curve distribution uniformity coefficients of the fluorescence time sequence curve sequence to obtain a curve distribution uniformity coefficient set, and identifying a target probe concentration-volume ratio combination corresponding to the minimum curve distribution uniformity coefficient and a target fluorescence time sequence curve sequence, and carrying out fluorescence intensity change detection on the current to-be-detected ion solution by using the target probe concentration-volume ratio combination to obtain a current fluorescence time sequence curve, identifying similar ion test concentrations, obtaining the optimal probe pre-test concentration of the similar ion test concentrations, and carrying out fluorescence detection according to the optimal probe pre-test concentration. According to the invention, the setting accuracy of the probe concentration in the fluorescence detection process of the heavy metal ion concentration can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of fluorescence detection of heavy metal ions, and particularly to a fluorescence detection method and system for the concentration of heavy metal ions. Background Art

[0002] With the rapid development of industrialization, heavy metal pollution has become one of the severe challenges faced by the global ecological environment. Due to the characteristics of long half-life, difficult degradation, and easy bioaccumulation in the food chain of heavy metal ions, they pose a serious threat to human health and environmental safety.

[0003] Fluorescence detection technology has received extensive attention in the field of heavy metal ion detection due to its advantages such as high sensitivity, rapid response, simple operation, and low cost. In practical applications, the selection of the concentration of the fluorescence probe has an important impact on the accuracy and economy of the detection results. On the one hand, too low a probe concentration may lead to weak detection signals, making it difficult to accurately determine the concentration of heavy metal ions and reducing the sensitivity and reliability of the detection; on the other hand, too high a probe concentration will not only increase the detection cost and cause waste of the probe, but may also introduce non-specific fluorescence signals, interfere with the detection results, and even cause fluorescence quenching phenomena, further reducing the accuracy of the detection. Therefore, there is a problem of inaccurate setting of the probe concentration in the current fluorescence detection process of heavy metal ion concentration. Summary of the Invention

[0004] The present invention provides a fluorescence detection method and system for the concentration of heavy metal ions, and its main purpose is to improve the accuracy of setting the probe concentration in the fluorescence detection process of heavy metal ion concentration.

[0005] To achieve the above object, a fluorescence detection method for the concentration of heavy metal ions provided by the present invention includes: Obtain a probe preliminary test gradient concentration sequence and an ion test gradient concentration sequence, and sequentially extract the probe preliminary test gradient concentrations in the probe preliminary test gradient concentration sequence, where the probe preliminary test gradient concentration sequence refers to a sequence composed of probe gradient concentrations for pre-testing heavy metal ion solutions; Obtain a preliminary test gradient volume ratio sequence, and perform permutation and combination according to the preliminary test gradient volume ratio sequence and the probe preliminary test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; Sequentially extract the probe concentration-volume ratio combinations in the probe concentration-volume ratio combination sequence; Perform a fluorescence intensity change test according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence time-sequence curve sequence corresponding to the ion test gradient concentration sequence; Calculate the curve distribution uniformity coefficient of the fluorescence time-sequence curve sequence to obtain a curve distribution uniformity coefficient set; Extract the minimum curve distribution uniformity coefficient from the curve distribution uniformity coefficients, and identify the target probe concentration-volume ratio combination and the target fluorescence time-sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient; Obtain the current ion solution to be measured, and detect the fluorescence intensity change of the current ion solution to be measured by using the target probe concentration-volume ratio combination to obtain the current fluorescence time-sequence curve; Identify the similar fluorescence time-sequence curve of the current fluorescence time-sequence curve in the target fluorescence time-sequence curve sequence, and identify the similar ion test concentration corresponding to the similar fluorescence time-sequence curve; Obtain the optimal probe preliminary test concentration of the similar ion test concentration, and perform fluorescence detection on the current ion solution to be measured according to the optimal probe preliminary test concentration.

[0006] Optionally, the obtaining the probe preliminary test gradient concentration sequence and the ion test gradient concentration sequence includes: Receive the probe preliminary test concentration threshold and the probe preliminary test fine level input by the user, and calculate the probe preliminary test concentration gradient according to the probe preliminary test concentration threshold by the following formula, where the smaller the probe preliminary test fine level, the higher the fine degree of pre-testing the heavy metal ion solution: ; Wherein, represents the probe preliminary test concentration gradient, represents the probe preliminary test concentration threshold, represents the natural constant, represents the probe preliminary test fine level; Set the probe preliminary test gradient concentration sequence according to the probe preliminary test concentration gradient; Obtain the heavy metal ion concentration threshold and the ion test fine level; According to the heavy metal ion concentration threshold and the ion test fine level, calculate the ion test concentration gradient by the following formula, where the smaller the ion test fine level, the higher the fine degree of pre-testing the heavy metal ion solution: ; Wherein, represents the ion test concentration gradient, represents the heavy metal ion concentration threshold, represents the ion test fine level; Set the ion test gradient concentration sequence according to the ion test concentration gradient.

[0007] Optionally, the arranging and combining according to the preliminary test gradient volume ratio sequence and the probe preliminary test gradient concentration sequence to obtain the probe concentration-volume ratio combination sequence includes: Successively extract the preliminary test volume ratios in the preliminary test gradient volume ratio sequence, Combined with each probe pre - test gradient concentration in the probe pre - test gradient concentration sequence according to the pre - test volume ratio, a probe concentration - volume ratio combination sequence is obtained, where the number of combinations of probe concentration - volume ratio combinations in the probe concentration - volume ratio combination sequence is equal to , where represents the number of pre - test gradient volume ratios in the pre - test gradient volume ratio sequence, represents the number of probe pre - test gradient concentrations in the probe pre - test gradient concentration sequence.

[0008] Optionally, the fluorescence intensity change test is performed according to the probe concentration - volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence time - series curve sequence corresponding to the ion test gradient concentration sequence, including: Successively extract the ion test gradient concentrations in the ion test gradient concentration sequence, and configure an ion test gradient solution per unit volume according to the ion test gradient concentration, where the unit volume is 100 mL; Identify the pre - test volume ratio in the probe concentration - volume ratio combination, and calculate the probe pre - test solution volume according to the pre - test volume ratio and the unit volume using the following formula: ; where represents the probe pre - test solution volume, represents the pre - test volume ratio; Identify the probe pre - test concentration in the probe concentration - volume ratio combination, and configure a probe pre - test solution according to the probe pre - test concentration and the probe pre - test solution volume; Add the probe pre - test solution to the ion test gradient solution to obtain a probe - ion mixed solution; Perform real - time monitoring of the fluorescence intensity of the probe - ion mixed solution to obtain a fluorescence time - series curve, where the fluorescence time - series curve refers to the curve of the fluorescence intensity of the probe - ion mixed solution changing with time within a predetermined time; Collect the fluorescence time - series curves corresponding to each ion test gradient concentration to obtain a fluorescence time - series curve sequence.

[0009] Optionally, calculating the curve distribution uniformity coefficient of the fluorescence time - series curve sequence to obtain a curve distribution uniformity coefficient set includes: Obtain a fluorescence value time series, and perform time marking on each fluorescence time - series curve in the fluorescence time - series curve sequence according to the fluorescence value time series to obtain multiple groups of fluorescence - marked point sequences, where a group of fluorescence - marked point sequences refers to the sequence composed of fluorescence - marked points obtained after time marking on a fluorescence time - series curve; Identify the fluorescence intensity slope corresponding to each fluorescence - labeled point in the fluorescence - labeled point sequence to obtain a fluorescence intensity slope sequence; Calculate the slope change value of each fluorescence - labeled point in the fluorescence - labeled point sequence using a pre - constructed slope change formula to obtain a set of slope change values; Identify the maximum slope change value in the set of slope change values, identify the intermediate fluorescence - labeled point corresponding to the maximum slope change value, and identify the intermediate fluorescence intensity slope corresponding to the intermediate fluorescence - labeled point; Classify the fluorescence - labeled point sequence according to the intermediate fluorescence - labeled point to obtain a pre - placed fluorescence - labeled point sequence and a post - placed fluorescence - labeled point sequence, where the pre - placed fluorescence - labeled point sequence refers to the sequence composed of the fluorescence - labeled points in front of the intermediate fluorescence - labeled point in the fluorescence - labeled point sequence, and the post - placed fluorescence - labeled points refer to the sequence composed of the fluorescence - labeled points behind the intermediate fluorescence - labeled point in the fluorescence - labeled point sequence; Collect the pre - placed fluorescence - labeled point sequences and post - placed fluorescence - labeled point sequences corresponding to each fluorescence time - series curve to obtain multiple groups of pre - placed fluorescence - labeled point sequences and multiple groups of post - placed fluorescence - labeled point sequences; According to the multiple groups of pre - placed fluorescence - labeled point sequences, calculate the pre - placed distribution uniformity coefficient using the following formula: ; where, represents the pre - placed distribution uniformity coefficient, represents the pre - placed weight, represents the number of groups of pre - placed fluorescence - labeled point sequences or the number of fluorescence time - series curves in the fluorescence time - series curve sequence, represents the fluorescence intensity of the first pre - placed fluorescence - labeled point in the (p + 1) - th group of pre - placed fluorescence - labeled point sequences, represents the fluorescence intensity of the first pre - placed fluorescence - labeled point in the p - th group of pre - placed fluorescence - labeled point sequences, represents the fluorescence intensity of the first pre - placed fluorescence - labeled point in the P - th group of pre - placed fluorescence - labeled point sequences, represents the fluorescence intensity of the first pre - placed fluorescence - labeled point in the first group of pre - placed fluorescence - labeled point sequences, represents the -th pre - placed fluorescence - labeled point in the (p + 1) - th group of pre - placed fluorescence - labeled point sequences, represents the -th pre - placed fluorescence - labeled point in the p - th group of pre - placed fluorescence - labeled point sequences, represents the -th pre - placed fluorescence - labeled point in the first group of pre - placed fluorescence - labeled point sequences, represents the -th pre - placed fluorescence - labeled point in the P - th group of pre - placed fluorescence - labeled point sequences, represents the total number of pre - fluorescent marker points in the pre - fluorescent marker point sequence; According to the multiple groups of post - fluorescent marker point sequences, the post - distribution uniformity coefficient is calculated using the following formula: ; where, represents the post - distribution uniformity coefficient, represents the post - weight, represents the number of groups of post - fluorescent marker point sequences or the number of fluorescence time - series curves in the fluorescence time - series curve sequence, represents the fluorescence intensity of the first post - fluorescent marker point in the (p + 1)-th group of post - fluorescent marker point sequences, represents the fluorescence intensity of the first post - fluorescent marker point in the p - th group of post - fluorescent marker point sequences, represents the fluorescence intensity of the first post - fluorescent marker point in the P - th group of post - fluorescent marker point sequences, represents the fluorescence intensity of the first post - fluorescent marker point in the first group of post - fluorescent marker point sequences, represents the -th post - fluorescent marker point in the (p + 1)-th group of post - fluorescent marker point sequences, represents the -th post - fluorescent marker point in the p - th group of post - fluorescent marker point sequences, represents the -th post - fluorescent marker point in the first group of post - fluorescent marker point sequences, represents the -th post - fluorescent marker point in the P - th group of post - fluorescent marker point sequences, represents the total number of post - fluorescent marker points in the post - fluorescent marker point sequence; According to the pre - distribution uniformity coefficient and the post - distribution uniformity coefficient, the curve distribution uniformity coefficient is calculated using the following formula: ; where, represents the curve distribution uniformity coefficient; The curve distribution uniformity coefficients of each probe concentration - volume ratio combination are collected to obtain a curve distribution uniformity coefficient set.

[0010] Optionally, the slope change formula is as follows: ; where, represents the slope change value of the first fluorescent marker point in the fluorescent marker point sequence, represents the fluorescence intensity slope of the first fluorescent marker point in the fluorescent marker point sequence, represents the fluorescence intensity slope of the second fluorescence marker point in the fluorescence marker point sequence, represents the slope change value of the i-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the (i - 1)-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the i-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the (i + 1)-th fluorescence marker point in the fluorescence marker point sequence, represents the total number of fluorescence marker points in the fluorescence marker point sequence, represents the slope change value of the I-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the (I - 1)-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the I-th fluorescence marker point in the fluorescence marker point sequence, represents the absolute value symbol.

[0011] Optionally, identifying the similar fluorescence time sequence curve of the current fluorescence time sequence curve in the target fluorescence time sequence curve sequence includes: Performing time marking on the current fluorescence time sequence curve according to the fluorescence value time sequence to obtain a current fluorescence marker point sequence, wherein the monitoring time of the fluorescence intensity change of the current fluorescence time sequence curve is the predetermined time; Sequentially extracting target fluorescence time sequence curves in the target fluorescence time sequence curve sequence, and calculating the curve difference value between the target fluorescence time sequence curve and the current fluorescence time sequence curve by using a pre-constructed curve difference formula to obtain a curve difference value set; Identifying the minimum curve difference value in the curve difference value set, and identifying the similar fluorescence time sequence curve corresponding to the minimum curve difference value in the target fluorescence time sequence curve sequence.

[0012] Optionally, the curve difference formula is as follows: ; wherein, represents the curve difference value, represents the fluorescence intensity of the j-th current fluorescence marker point in the current fluorescence marker point sequence, represents the fluorescence intensity of the j-th fluorescence marker point in the target fluorescence time sequence curve.

[0013] Optionally, obtaining the optimal probe preliminary concentration of the similar ion test concentration includes: Calculating the optimal probe preliminary concentration by using the similar ion test concentration according to a pre-constructed probe concentration formula, wherein the probe concentration formula is as follows: ; Among them, represents the optimal probe pre-test concentration, represents the ion ratio coefficient, represents the similar ion test concentration.

[0014] To achieve the above object, the present invention also provides a fluorescence detection system for heavy metal ion concentration, including: A probe concentration-volume ratio combination extraction module, which is used to obtain a probe pre-test gradient concentration sequence and an ion test gradient concentration sequence, sequentially extract the probe pre-test gradient concentration in the probe pre-test gradient concentration sequence, where the probe pre-test gradient concentration sequence refers to a sequence composed of probe gradient concentrations for pre-testing a heavy metal ion solution; obtain a pre-test gradient volume ratio sequence, and perform permutation and combination according to the pre-test gradient volume ratio sequence and the probe pre-test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; sequentially extract the probe concentration-volume ratio combination in the probe concentration-volume ratio combination sequence; A target probe concentration-volume ratio combination recognition module, which is used to perform fluorescence intensity change tests according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence time sequence curve sequence corresponding to the ion test gradient concentration sequence; calculate the curve distribution uniformity coefficient of the fluorescence time sequence curve sequence to obtain a curve distribution uniformity coefficient set; extract the minimum curve distribution uniformity coefficient in the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence time sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient; A current fluorescence time sequence curve detection module, which is used to obtain a current ion solution to be measured, and use the target probe concentration-volume ratio combination to detect the fluorescence intensity change of the current ion solution to be measured to obtain a current fluorescence time sequence curve; A current ion solution to be measured fluorescence detection module, which is used to identify a similar fluorescence time sequence curve of the current fluorescence time sequence curve in the target fluorescence time sequence curve sequence, and identify the similar ion test concentration corresponding to the similar fluorescence time sequence curve; obtain the optimal probe pre-test concentration of the similar ion test concentration, and perform fluorescence detection on the current ion solution to be measured according to the optimal probe pre-test concentration.

[0015] To solve the above problems, the present invention also provides an electronic device, and the electronic device includes: A memory that stores at least one instruction; and a processor that executes the instruction stored in the memory to implement the above-mentioned fluorescence detection method for heavy metal ion concentration.

[0016] To solve the above problems, the present invention also provides a computer-readable storage medium storing at least one instruction, and the at least one instruction is executed by a processor in an electronic device to implement the above-mentioned fluorescence detection method for heavy metal ion concentration.

[0017] To solve the problems described in the background art, the present invention first needs to obtain a probe concentration-volume ratio combination for fluorescence intensity change testing. When obtaining the probe concentration-volume ratio combination, it is necessary to first obtain a probe preliminary test gradient concentration sequence and an ion test gradient concentration sequence, and then sequentially extract the probe preliminary test gradient concentrations from the probe preliminary test gradient concentration sequence. At this time, the probe concentration-volume ratio combination sequence can be obtained by arranging and combining according to the preliminary test gradient volume ratio sequence and the probe preliminary test gradient concentration sequence. Finally, the probe concentration-volume ratio combination is sequentially extracted from the probe concentration-volume ratio combination sequence to obtain the probe concentration-volume ratio combination. After obtaining the probe concentration-volume ratio combination, the fluorescence intensity change test can be directly performed according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain the fluorescence time sequence curve sequence corresponding to the ion test gradient concentration sequence. Since the distribution of the fluorescence time sequence curve sequence under different probe concentration-volume ratio combinations is different, the curve distribution uniformity coefficient of the fluorescence time sequence curve sequence can be calculated first to obtain a curve distribution uniformity coefficient set. Since the smaller the curve distribution uniformity coefficient, the more regular and uniform the distribution of the fluorescence time sequence curve sequence, the stronger the reference of the fluorescence time sequence curve sequence at this time. Therefore, the minimum curve distribution uniformity coefficient can be extracted from the curve distribution uniformity coefficient set, and then the target probe concentration-volume ratio combination and the target fluorescence time sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient are identified. At this time, the current ion solution to be measured can be obtained, and then the fluorescence intensity change of the current ion solution to be measured is detected using the target probe concentration-volume ratio combination to obtain the current fluorescence time sequence curve. Since the heavy metal ion concentration corresponding to the current fluorescence time sequence curve is closest to that of the similar fluorescence time sequence curve, the similar fluorescence time sequence curve of the current fluorescence time sequence curve can be identified in the target fluorescence time sequence curve sequence, and then the similar ion test concentration corresponding to the similar fluorescence time sequence curve is identified. Finally, the optimal probe preliminary test concentration of the similar ion test concentration is obtained, and the current ion solution to be measured is subjected to fluorescence detection according to the optimal probe preliminary test concentration. Therefore, the present invention can improve the setting accuracy of the probe concentration in the process of fluorescence detection of heavy metal ion concentration. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic flowchart of a fluorescence detection method for heavy metal ion concentration provided by an embodiment of the present invention; Figure 2Functional module diagram of a fluorescence detection system for heavy metal ion concentration provided by an embodiment of the present invention; Figure 3 Structural schematic diagram of an electronic device for implementing the fluorescence detection method for heavy metal ion concentration provided by an embodiment of the present invention.

[0019] Explanation of reference numerals: 1. Electronic device; 10. Processor; 11. Memory; 12. Bus.

[0020] The realization, functional characteristics and advantages of the object of the present invention will be further described with reference to the embodiments and the accompanying drawings. Specific embodiments

[0021] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0022] An embodiment of the present application provides a fluorescence detection method for heavy metal ion concentration. The execution subject of the fluorescence detection method for heavy metal ion concentration includes, but is not limited to, at least one of electronic devices such as a server, a terminal, etc. that can be configured to execute the method provided by the embodiment of the present application. In other words, the fluorescence detection method for heavy metal ion concentration can be executed by software or hardware installed on a terminal device or a server device, and the software can be a blockchain platform. The server includes, but is not limited to: a single server, a server cluster, a cloud server or a cloud server cluster, etc.

[0023] Refer to Figure 1 As shown, it is a flowchart of a fluorescence detection method for heavy metal ion concentration provided by an embodiment of the present invention. In this embodiment, the fluorescence detection method for heavy metal ion concentration includes: S1. Obtain a probe pre-test gradient concentration sequence and an ion test gradient concentration sequence, and sequentially extract probe pre-test gradient concentrations in the probe pre-test gradient concentration sequence, where the probe pre-test gradient concentration sequence refers to a sequence composed of probe gradient concentrations for pre-testing the heavy metal ion concentration of a heavy metal ion solution.

[0024] It is understandable that the heavy metal ion solution can be , , Solutions of heavy metal ions such as the above. The pre-test refers to the pre-test process of the heavy metal ion solution before detecting the ion concentration of the heavy metal ion solution. Through the pre-test, the approximate ion concentration of the heavy metal ion solution can be obtained to determine the optimal probe solution concentration. See the following embodiments for details. The probe gradient concentration refers to the gradient probe concentration, and the probe pre-test gradient concentration sequence refers to the sequence composed of the gradient concentrations of the probe during the pre-test process. For example, the probe pre-test gradient concentration sequence can be: mol / L, mol / L, mol / L, mol / L, and so on. The ion test gradient concentration sequence refers to the sequence composed of the gradient concentrations of the heavy metal ions during the pre-test process. For example, when the heavy metal ion is , the ion test gradient concentration sequence can be: mol / L, mol / L, mol / L, mol / L, and so on.

[0025] In the embodiments of the present invention, obtaining the probe pre-test gradient concentration sequence and the ion test gradient concentration sequence includes: Receiving the probe pre-test concentration threshold and the probe pre-test fine level input by the user, and calculating the probe pre-test concentration gradient according to the probe pre-test concentration threshold using the following formula. Among them, the smaller the probe pre-test fine level, the higher the fine degree of the pre-test of the heavy metal ion solution: ;

[0026] Among them, represents the probe pre-test concentration gradient, represents the probe pre-test concentration threshold, represents the natural constant, represents the probe pre-test fine level; Setting the probe pre-test gradient concentration sequence according to the probe pre-test concentration gradient; Obtaining the heavy metal ion concentration threshold and the ion test fine level; According to the heavy metal ion concentration threshold and the ion test fine level, calculating the ion test concentration gradient using the following formula. Among them, the smaller the ion test fine level, the higher the fine degree of the pre-test of the heavy metal ion solution: ;

[0027] Among them, represents the ion test concentration gradient, Represents the heavy metal ion concentration threshold, Represents the ion test fineness level; Set the ion test gradient concentration sequence according to the ion test concentration gradient.

[0028] It should be understood that the probe pre-test concentration threshold refers to the maximum value of the probe concentration during the pre-test process, and the probe pre-test fineness level refers to the refined level of setting the probe concentration. The probe pre-test concentration gradient refers to the probe concentration change gradient corresponding to the probe pre-test gradient concentration sequence. For example: when the probe pre-test concentration gradient is: mol / L, the probe pre-test gradient concentration sequence can be: mol / L, mol / L, mol / L, mol / L and so on.

[0029] Furthermore, the heavy metal ion concentration threshold refers to the maximum value of the heavy metal ion concentration during the pre-test process, and the ion test fineness level refers to the refined level of setting the heavy metal ion concentration. The ion test concentration gradient refers to the ion concentration change gradient corresponding to the ion test gradient concentration sequence. For example: when the ion test concentration gradient is mol / L, the ion test gradient concentration sequence can be: mol / L, mol / L, mol / L, mol / L and so on.

[0030] S2. Obtain the pre-test gradient volume ratio sequence, and perform permutation and combination according to the pre-test gradient volume ratio sequence and the probe pre-test gradient concentration sequence to obtain the probe concentration-volume ratio combination sequence.

[0031] Furthermore, the pre-test gradient volume ratio sequence refers to the sequence composed of the solution volume ratios of the probe pre-test test solution and the ion test gradient solution during the pre-test process. The probe concentration-volume ratio combination sequence refers to the combination sequence composed of the pre-test gradient volume ratio and the probe pre-test gradient concentration.

[0032] For example, when the pre-test gradient volume ratio is: 1, 1.2, 1.4, 1.6, and the probe pre-test gradient concentration is: mol / L, mol / L, mol / L, the probe concentration-volume ratio combination sequence can be: mol / L - 1; mol / L - 1.2; mol / L - 1.4; mol / L - 1.6; mol / L - 1, mol / L - 1.2 and so on.

[0033] In the embodiments of the present invention, arranging and combining according to the pre - test gradient volume ratio sequence and the probe pre - test gradient concentration sequence to obtain a probe concentration - volume ratio combination sequence, including: Successively extracting pre - test volume ratios in the pre - test gradient volume ratio sequence, Combining each pre - test volume ratio with each probe pre - test gradient concentration in the probe pre - test gradient concentration sequence to obtain a probe concentration - volume ratio combination sequence, where the number of combinations of probe concentration - volume ratio combinations in the probe concentration - volume ratio combination sequence is equal to , where represents the number of pre - test gradient volume ratios in the pre - test gradient volume ratio sequence, represents the number of probe pre - test gradient concentrations in the probe pre - test gradient concentration sequence.

[0034] S3. Successively extracting probe concentration - volume ratio combinations in the probe concentration - volume ratio combination sequence.

[0035] S4. Conducting fluorescence intensity change tests according to the probe concentration - volume ratio combinations and the ion test gradient concentration sequence to obtain a fluorescence time - series curve sequence corresponding to the ion test gradient concentration sequence.

[0036] It is understandable that the fluorescence intensity change test refers to testing the change of the fluorescence intensity of the solution over time under the probe concentration - volume ratio combination and the ion test gradient concentration. The fluorescence time - series curve sequence refers to a sequence composed of the change curves of the fluorescence intensity of the solution over time under the test conditions of the probe concentration - volume ratio combination and the ion test gradient concentration.

[0037] In the embodiments of the present invention, conducting fluorescence intensity change tests according to the probe concentration - volume ratio combinations and the ion test gradient concentration sequence to obtain a fluorescence time - series curve sequence corresponding to the ion test gradient concentration sequence, including: Successively extracting ion test gradient concentrations in the ion test gradient concentration sequence, and configuring an ion test gradient solution with a unit volume according to the ion test gradient concentration, where the unit volume is 100 mL; Identifying the pre - test test volume ratio in the probe concentration - volume ratio combination, and calculating the probe pre - test solution volume according to the pre - test test volume ratio and the unit volume using the following formula: ;

[0038] where Indicates the volume of the probe preliminary test solution, Indicates the preliminary test volume ratio; Identify the probe preliminary test concentration in the probe concentration-volume ratio combination, and configure a probe preliminary test solution according to the probe preliminary test concentration and the volume of the probe preliminary test solution; Add the probe preliminary test solution to the ion test gradient solution to obtain a probe ion mixed solution; Perform real-time monitoring of the fluorescence intensity of the probe ion mixed solution to obtain a fluorescence time sequence curve, where the fluorescence time sequence curve refers to the curve of the fluorescence intensity of the probe ion mixed solution changing with time within a predetermined time; Collect the fluorescence time sequence curves corresponding to each ion test gradient concentration to obtain a fluorescence time sequence curve sequence.

[0039] Further, the ion test gradient solution refers to a solution with a heavy metal ion concentration of the ion test gradient concentration. The preliminary test volume ratio refers to the preliminary test gradient volume ratio in the probe concentration-volume ratio combination. The volume of the probe preliminary test solution refers to the volume of the probe preliminary test solution during the preliminary test process. The probe preliminary test concentration refers to the probe preliminary test gradient concentration in the probe concentration-volume ratio combination. The probe preliminary test solution refers to a probe solution used to test the approximate heavy metal ion concentration of the ion test gradient solution during the preliminary test process. The predetermined time can be 100s.

[0040] S5. Calculate the curve distribution uniformity coefficient of the fluorescence time sequence curve sequence to obtain a curve distribution uniformity coefficient set.

[0041] It is understandable that the curve distribution uniformity coefficient refers to a coefficient reflecting the degree of uniformity of the fluorescence time sequence curve distribution in the fluorescence time sequence curve sequence. The smaller the curve distribution uniformity coefficient, the more uniform the distribution of each fluorescence time sequence curve in the fluorescence time sequence curve sequence, and the closer the distance between adjacent fluorescence time sequence curves. The curve distribution uniformity coefficient set refers to the set of curve distribution uniformity coefficients corresponding to each probe concentration-volume ratio combination.

[0042] In the embodiment of the present invention, the calculation of the curve distribution uniformity coefficient of the fluorescence time sequence curve sequence to obtain a curve distribution uniformity coefficient set includes: Obtain a fluorescence value time sequence, and perform time marking on each fluorescence time sequence curve in the fluorescence time sequence curve sequence according to the fluorescence value time sequence to obtain multiple groups of fluorescence marker point sequences, where a group of fluorescence marker point sequences refers to a sequence composed of fluorescence marker points obtained after time marking on a fluorescence time sequence curve; Identify the fluorescence intensity slope corresponding to each fluorescence marker point in the fluorescence marker point sequence to obtain a fluorescence intensity slope sequence; Calculate the slope change value of each fluorescence marker point in the fluorescence marker point sequence using a pre-constructed slope change formula to obtain a set of slope change values; Identify the maximum slope change value in the set of slope change values, identify the intermediate fluorescence marker point corresponding to the maximum slope change value, and identify the intermediate fluorescence intensity slope corresponding to the intermediate fluorescence marker point; Classify the fluorescence marker point sequence according to the intermediate fluorescence marker point to obtain a preposed fluorescence marker point sequence and a postposed fluorescence marker point sequence, where the preposed fluorescence marker point sequence refers to the sequence composed of the fluorescence marker points in the fluorescence marker point sequence that are in front of the intermediate fluorescence marker point, and the postposed fluorescence marker points refer to the sequence composed of the fluorescence marker points in the fluorescence marker point sequence that are behind the intermediate fluorescence marker point; Collect the preposed fluorescence marker point sequences and postposed fluorescence marker point sequences corresponding to each fluorescence time sequence curve to obtain multiple groups of preposed fluorescence marker point sequences and multiple groups of postposed fluorescence marker point sequences; Calculate the preposed distribution uniformity coefficient according to the multiple groups of preposed fluorescence marker point sequences using the following formula: ;

[0043] where, represents the preposed distribution uniformity coefficient, represents the preposed weight, represents the number of groups of preposed fluorescence marker point sequences or the number of fluorescence time sequence curves in the fluorescence time sequence curve sequence, represents the fluorescence intensity of the first preposed fluorescence marker point in the (p + 1)-th group of preposed fluorescence marker point sequences, represents the fluorescence intensity of the first preposed fluorescence marker point in the p-th group of preposed fluorescence marker point sequences, represents the fluorescence intensity of the first preposed fluorescence marker point in the P-th group of preposed fluorescence marker point sequences, represents the fluorescence intensity of the first preposed fluorescence marker point in the first group of preposed fluorescence marker point sequences, represents the -th preposed fluorescence marker point in the (p + 1)-th group of preposed fluorescence marker point sequences, represents the -th preposed fluorescence marker point in the p-th group of preposed fluorescence marker point sequences, represents the -th preposed fluorescence marker point in the first group of preposed fluorescence marker point sequences, represents the -th preposed fluorescence marker point in the P-th group of preposed fluorescence marker point sequences, represents the total number of preposed fluorescence marker points in the preposed fluorescence marker point sequence; According to the multiple groups of post - fluorescent - labeled dot sequences, use the following formula to calculate the post - distribution uniformity coefficient: ;

[0044] Among them, represents the post - distribution uniformity coefficient, represents the post - weight, represents the number of groups of post - fluorescent - labeled dot sequences or the number of fluorescence time - sequence curves in the fluorescence time - sequence curve sequence, represents the fluorescence intensity of the first post - fluorescent - labeled dot in the (p + 1)-th group of post - fluorescent - labeled dot sequences, represents the fluorescence intensity of the first post - fluorescent - labeled dot in the p - th group of post - fluorescent - labeled dot sequences, represents the fluorescence intensity of the first post - fluorescent - labeled dot in the P - th group of post - fluorescent - labeled dot sequences, represents the fluorescence intensity of the first post - fluorescent - labeled dot in the first group of post - fluorescent - labeled dot sequences, represents the -th post - fluorescent - labeled dot in the (p + 1)-th group of post - fluorescent - labeled dot sequences, represents the -th post - fluorescent - labeled dot in the p - th group of post - fluorescent - labeled dot sequences, represents the -th post - fluorescent - labeled dot in the first group of post - fluorescent - labeled dot sequences, represents the -th post - fluorescent - labeled dot in the P - th group of post - fluorescent - labeled dot sequences, represents the total number of post - fluorescent - labeled dots in the post - fluorescent - labeled dot sequence; According to the pre - distribution uniformity coefficient and the post - distribution uniformity coefficient, use the following formula to calculate the curve - distribution uniformity coefficient: ;

[0045] Among them, represents the curve - distribution uniformity coefficient; Collect the curve - distribution uniformity coefficients of each probe concentration - volume ratio combination to obtain a curve - distribution uniformity coefficient set.

[0046] It is understandable that the fluorescence value-taking time series refers to the time series obtained by time-marking the fluorescence time sequence curve. For example, when the predetermined time is 100 s, the fluorescence value-taking time series can be 10 s, 20 s, 30 s, 40 s,..., 100 s. The fluorescence marker point sequence refers to the sequence composed of the fluorescence marker points on the fluorescence time sequence curve after time-marking. The fluorescence intensity slope refers to the curve slope at the fluorescence marker points on the fluorescence time sequence curve, and the fluorescence intensity slope sequence refers to the sequence composed of the curve slopes at each fluorescence marker point in the corresponding fluorescence marker point sequence on a fluorescence time sequence curve. The slope change value refers to the comprehensive slope change value between the fluorescence intensity slope of the fluorescence marker point and one or two adjacent fluorescence marker points, as detailed in the following embodiments. The slope change value set refers to the set of slope change values corresponding to each fluorescence marker point in the fluorescence marker point sequence.

[0047] It is understandable that the intermediate fluorescence marker point refers to the fluorescence marker point corresponding to the maximum slope change value. Since during the fluorescence probe detection process, the fluorescence intensity change trend of the fluorescence time sequence curve should first gradually increase with the extension of time, and when it increases to a certain specific time, the fluorescence intensity should no longer change significantly (at this time, the binding of the probe to heavy metal ions has approached saturation), therefore, the intermediate fluorescence marker point should be at this specific time (i.e., at the inflection point of the fluorescence time sequence curve). The intermediate fluorescence intensity slope refers to the fluorescence intensity slope at the intermediate fluorescence marker point.

[0048] It is interpretable that the pre-distribution uniformity coefficient refers to the degree of distribution uniformity of the first half of the multiple fluorescence time sequence curves corresponding to the multiple pre-fluorescence marker point sequences, and the post-distribution uniformity coefficient refers to the degree of distribution uniformity of the second half of the multiple fluorescence time sequence curves corresponding to the multiple post-fluorescence marker point sequences.

[0049] In the embodiments of the present invention, the slope change formula is as follows: ;

[0050] Wherein, represents the slope change value of the first fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the first fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the second fluorescence marker point in the fluorescence marker point sequence, represents the slope change value of the i-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the (i - 1)-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the i-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the (i + 1)-th fluorescence marker point in the fluorescence marker point sequence, represents the total number of fluorescence marker points in the fluorescence marker point sequence, represents the slope change value of the I-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the (I - 1)-th fluorescence marker point in the fluorescence marker point sequence, represents the fluorescence intensity slope of the I-th fluorescence marker point in the fluorescence marker point sequence, represents the absolute value symbol.

[0051] S6. Extract the minimum curve distribution uniformity coefficient from the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence time-sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient.

[0052] Further, the minimum curve distribution uniformity coefficient refers to the minimum curve distribution uniformity coefficient in the curve distribution uniformity coefficient set. The target probe concentration-volume ratio combination refers to the probe concentration-volume ratio combination corresponding to the minimum curve distribution uniformity coefficient. The target fluorescence time-sequence curve sequence refers to the fluorescence time-sequence curve sequence corresponding to the target probe concentration-volume ratio combination.

[0053] S7. Obtain the current ion solution to be measured, and use the target probe concentration-volume ratio combination to detect the change in fluorescence intensity of the current ion solution to be measured, so as to obtain the current fluorescence time-sequence curve.

[0054] It can be understood that the current ion solution to be measured refers to the solution that currently needs to be detected for the concentration of heavy metal ions by fluorescence. The current fluorescence time-sequence curve refers to the curve of the change in fluorescence intensity of the current ion solution to be measured over time.

[0055] S8. Identify the similar fluorescence time-sequence curve of the current fluorescence time-sequence curve in the target fluorescence time-sequence curve sequence, and identify the similar ion test concentration corresponding to the similar fluorescence time-sequence curve.

[0056] Further, the similar fluorescence time-sequence curve refers to the target fluorescence time-sequence curve in the target fluorescence time-sequence curve sequence that is most similar to the current fluorescence time-sequence curve. The similar ion test concentration refers to the ion test gradient concentration corresponding to the similar fluorescence time-sequence curve.

[0057] In the embodiment of the present invention, identifying the similar fluorescence time-sequence curve of the current fluorescence time-sequence curve in the target fluorescence time-sequence curve sequence includes: Mark the time of the current fluorescence time-sequence curve according to the fluorescence value time sequence to obtain the current fluorescence marker point sequence, where the monitoring time of the change in fluorescence intensity of the current fluorescence time-sequence curve is the predetermined time; Extract the target fluorescence time-sequence curves from the target fluorescence time-sequence curve sequence in turn, and calculate the curve difference value between the target fluorescence time-sequence curve and the current fluorescence time-sequence curve by using the pre-constructed curve difference formula to obtain a set of curve difference values; Identify the minimum curve difference value in the set of curve difference values, and identify the similar fluorescence time-sequence curve corresponding to the minimum curve difference value in the target fluorescence time-sequence curve sequence.

[0058] It can be understood that the curve difference value refers to the degree of difference between the target fluorescence time-sequence curve and the current fluorescence time-sequence curve.

[0059] In the embodiment of the present invention, the curve difference formula is as follows: ;

[0060] Wherein, represents the curve difference value, represents the fluorescence intensity of the j-th current fluorescence marker point in the current fluorescence marker point sequence, represents the fluorescence intensity of the j-th fluorescence marker point in the target fluorescence time-sequence curve.

[0061] S9. Obtain the optimal probe preliminary concentration for the similar ion test concentration, and perform fluorescence detection on the current ion solution to be measured according to the optimal probe preliminary concentration.

[0062] It can be understood that the optimal probe preliminary concentration refers to the optimal probe solution concentration of the heavy metal ion solution corresponding to the similar ion test concentration.

[0063] Specifically, when the approximate concentration of the heavy metal ions in the heavy metal ion solution is known, the binding process between the probe and the heavy metal ions can be divided into two parts. The first part is that the fluorescence intensity increases with the passage of time (at this time, since some heavy metal ions have not completed binding with the probe, the fluorescence intensity has not reached the strongest yet), and the second part is that the fluorescence intensity does not change significantly with the passage of time (at this time, since the heavy metal ions are completely bound to the probe, the fluorescence intensity tends to be stable). When the heavy metal ions are completely bound to the probe, no extra probe is needed. Therefore, after knowing the approximate concentration of the heavy metal ion solution, an optimal probe solution concentration can be determined to avoid an excessive amount of the probe.

[0064] Furthermore, since the curve change trends of the current fluorescence time-sequence curve and the similar fluorescence time-sequence curve are the most similar, the ion test gradient concentration corresponding to the similar fluorescence time-sequence curve can be used as the approximate concentration of the current ion solution to be measured.

[0065] In the embodiments of the present invention, the obtaining of the optimal probe preliminary concentration for the similar ion test concentration includes: According to the pre-constructed probe concentration formula, the optimal probe preliminary concentration is calculated using the similar ion test concentration, where the probe concentration formula is as follows: ;

[0066] where, represents the optimal probe preliminary concentration, represents the ion proportion coefficient, represents the similar ion test concentration.

[0067] It can be understood that the ion proportion coefficient can be 1.2, that is, when the rough heavy metal ion concentration of the current ion solution to be measured is the optimal probe preliminary concentration is 1.2 times the similar ion test concentration.

[0068] To solve the problems described in the background art, the present invention first needs to obtain a probe concentration-volume ratio combination for fluorescence intensity change testing. When obtaining the probe concentration-volume ratio combination, it is necessary to first obtain a probe preliminary test gradient concentration sequence and an ion test gradient concentration sequence, and then sequentially extract the probe preliminary test gradient concentrations from the probe preliminary test gradient concentration sequence. At this time, the probe concentration-volume ratio combination sequence can be obtained by arranging and combining according to the preliminary test gradient volume ratio sequence and the probe preliminary test gradient concentration sequence. Finally, the probe concentration-volume ratio combination is sequentially extracted from the probe concentration-volume ratio combination sequence to obtain the probe concentration-volume ratio combination. When the probe concentration-volume ratio combination is obtained, the fluorescence intensity change test can be directly performed according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain the fluorescence time sequence curve sequence corresponding to the ion test gradient concentration sequence. Since the distribution of the fluorescence time sequence curve sequence under different probe concentration-volume ratio combinations is different, the curve distribution uniformity coefficient of the fluorescence time sequence curve sequence can be calculated first to obtain a curve distribution uniformity coefficient set. Since the smaller the curve distribution uniformity coefficient, the more regular and uniform the distribution of the fluorescence time sequence curve sequence, the stronger the reference of the fluorescence time sequence curve sequence at this time. Therefore, the minimum curve distribution uniformity coefficient can be extracted from the curve distribution uniformity coefficient set, and then the target probe concentration-volume ratio combination and the target fluorescence time sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient can be identified. At this time, the current ion solution to be measured can be obtained, and then the fluorescence intensity change of the current ion solution to be measured is detected using the target probe concentration-volume ratio combination to obtain the current fluorescence time sequence curve. Since the heavy metal ion concentration corresponding to the current fluorescence time sequence curve is closest to that of the similar fluorescence time sequence curve, the similar fluorescence time sequence curve of the current fluorescence time sequence curve can be identified in the target fluorescence time sequence curve sequence, and then the similar ion test concentration corresponding to the similar fluorescence time sequence curve can be identified. Finally, the optimal probe preliminary test concentration of the similar ion test concentration is obtained, and the current ion solution to be measured is fluorescence-detected according to the optimal probe preliminary test concentration. Therefore, the present invention can improve the setting accuracy of the probe concentration during the fluorescence detection of heavy metal ion concentration.

[0069] As Figure 2 shown, it is a functional module diagram of a fluorescence detection system for heavy metal ion concentration provided by an embodiment of the present invention.

[0070] The fluorescence detection system 100 for heavy metal ion concentration according to the present invention can be installed in an electronic device. According to the functions achieved, the fluorescence detection system 100 for heavy metal ion concentration may include a probe concentration-volume ratio combination extraction module 101, a target probe concentration-volume ratio combination identification module 102, a current fluorescence time sequence curve detection module 103, and a current ion solution to be detected fluorescence detection module 104. The modules in the present invention may also be referred to as units, which refer to a series of computer program segments that can be executed by an electronic device processor and can complete fixed functions, and are stored in the memory of the electronic device.

[0071] The probe concentration-volume ratio combination extraction module 101 is configured to obtain a probe preliminary test gradient concentration sequence and an ion test gradient concentration sequence, and sequentially extract probe preliminary test gradient concentrations in the probe preliminary test gradient concentration sequence, where the probe preliminary test gradient concentration sequence refers to a sequence composed of probe gradient concentrations for pre-testing a heavy metal ion solution; obtain a preliminary test gradient volume ratio sequence, and perform permutation and combination according to the preliminary test gradient volume ratio sequence and the probe preliminary test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; and sequentially extract probe concentration-volume ratio combinations in the probe concentration-volume ratio combination sequence. The target probe concentration-volume ratio combination identification module 102 is configured to perform a fluorescence intensity change test according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence time sequence curve sequence corresponding to the ion test gradient concentration sequence; calculate the curve distribution uniformity coefficient of the fluorescence time sequence curve sequence to obtain a curve distribution uniformity coefficient set; extract the minimum curve distribution uniformity coefficient in the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence time sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient. The current fluorescence time sequence curve detection module 103 is configured to obtain a current ion solution to be detected, and perform a fluorescence intensity change detection on the current ion solution to be detected by using the target probe concentration-volume ratio combination to obtain a current fluorescence time sequence curve. The current ion solution to be detected fluorescence detection module 104 is configured to identify a similar fluorescence time sequence curve of the current fluorescence time sequence curve in the target fluorescence time sequence curve sequence, and identify the similar ion test concentration corresponding to the similar fluorescence time sequence curve; obtain the optimal probe preliminary test concentration of the similar ion test concentration, and perform fluorescence detection on the current ion solution to be detected according to the optimal probe preliminary test concentration.

[0072] Specifically, each module in the fluorescence detection system 100 for heavy metal ion concentration in the embodiment of the present invention adopts the same technical means as those in the Figure 1 fluorescence detection method for heavy metal ion concentration described above, and can produce the same technical effects, which will not be elaborated here.

[0073] As shown Figure 3 in the figure, it is a schematic structural diagram of an electronic device for implementing a fluorescence detection method for heavy metal ion concentration provided by an embodiment of the present invention.

[0074] The electronic device 1 may include a processor 10, a memory 11, and a bus 12, and may also include a computer program stored in the memory 11 and executable on the processor 10, such as a program for the fluorescence detection method for heavy metal ion concentration.

[0075] Among them, the memory 11 includes at least one type of readable storage medium, and the readable storage medium includes flash memory, mobile hard disk, multimedia card, card-type memory (such as SD or DX memory, etc.), magnetic memory, magnetic disk, optical disk, etc. In some embodiments, the memory 11 may be an internal storage unit of the electronic device 1, such as the mobile hard disk of the electronic device 1. In other embodiments, the memory 11 may also be an external storage device of the electronic device 1, such as a plug-in mobile hard disk, a Smart Media Card (SMC), a Secure Digital (SD) card, a Flash Card, etc. equipped on the electronic device 1. Further, the memory 11 also includes the internal storage unit of the electronic device 1 and the external storage device. The memory 11 can not only be used to store application software installed in the electronic device 1 and various types of data, such as the code of the program for the fluorescence detection method for heavy metal ion concentration, etc., but also be used to temporarily store data that has been output or will be output.

[0076] In some embodiments, the processor 10 may be composed of integrated circuits. For example, it may be composed of a single packaged integrated circuit, or may be composed of multiple integrated circuits with the same or different functions, including a combination of one or more Central Processing Units (CPUs), microprocessors, digital processing chips, graphics processors, and various control chips. The processor 10 is the control core (Control Unit) of the electronic device, connecting various components of the entire electronic device through various interfaces and lines, and by running or executing programs or modules stored in the memory 11 (such as the program for the fluorescence detection method for heavy metal ion concentration, etc.), and calling data stored in the memory 11, to execute various functions of the electronic device 1 and process data.

[0077] The bus 12 may be a Peripheral Component Interconnect (PCI) bus, an Extended Industry Standard Architecture (EISA) bus, or the like. The bus 12 can be divided into an address bus, a data bus, a control bus, etc. The bus 12 is configured to implement the connection and communication between the memory 11 and at least one processor 10, etc.

[0078] Figure 3 Only an electronic device with components is shown. Those skilled in the art can understand that Figure 3 the shown structure does not constitute a limitation on the electronic device 1, and it may include fewer or more components than shown, or combine certain components, or have a different component layout.

[0079] For example, although not shown, the electronic device 1 may further include a power source (such as a battery) for supplying power to each component. Preferably, the power source can be logically connected to the at least one processor 10 through a power management device, so as to implement functions such as charging management, discharging management, and power consumption management through the power management device. The power source may also include any components such as one or more DC or AC power sources, a recharge device, a power failure detection circuit, a power converter or inverter, and a power status indicator. The electronic device 1 may also include various sensors, a Bluetooth module, a Wi-Fi module, etc., which will not be elaborated here.

[0080] Furthermore, the electronic device 1 may further include a network interface. Optionally, the network interface may include a wired interface and / or a wireless interface (such as a WI-FI interface, a Bluetooth interface, etc.), which is generally used to establish a communication connection between the electronic device 1 and other electronic devices.

[0081] Optionally, the electronic device 1 may further include a user interface. The user interface may be a display, an input unit (such as a keyboard), and optionally, the user interface may also be a standard wired interface or a wireless interface. Optionally, in some embodiments, the display may be an LED display, a liquid crystal display, a touch liquid crystal display, and an OLED (Organic Light-Emitting Diode) toucher, etc. Among them, the display may also be appropriately referred to as a display screen or a display unit, which is used to display the information processed in the electronic device 1 and to display a visual user interface.

[0082] The program for the fluorescence detection method of heavy metal ion concentration stored in the memory 11 in the electronic device 1 is a combination of multiple instructions. When running in the processor 10, it can achieve the following: Obtain the probe preliminary test gradient concentration sequence and the ion test gradient concentration sequence, and sequentially extract the probe preliminary test gradient concentrations in the probe preliminary test gradient concentration sequence. Herein, the probe preliminary test gradient concentration sequence refers to a sequence composed of probe gradient concentrations for pre-testing heavy metal ion solutions; Obtain the preliminary test gradient volume ratio sequence, and perform permutation and combination according to the preliminary test gradient volume ratio sequence and the probe preliminary test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; Sequentially extract the probe concentration-volume ratio combinations in the probe concentration-volume ratio combination sequence; Perform fluorescence intensity change tests according to the probe concentration-volume ratio combinations and the ion test gradient concentration sequence to obtain a fluorescence time-sequence curve sequence corresponding to the ion test gradient concentration sequence; Calculate the curve distribution uniformity coefficient of the fluorescence time-sequence curve sequence to obtain a curve distribution uniformity coefficient set; Extract the minimum curve distribution uniformity coefficient in the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence time-sequence curve sequence corresponding to the minimum curve distribution uniformity coefficient; Obtain the current ion solution to be measured, and use the target probe concentration-volume ratio combination to detect the fluorescence intensity change of the current ion solution to be measured to obtain the current fluorescence time-sequence curve; Identify the similar fluorescence time-sequence curve of the current fluorescence time-sequence curve in the target fluorescence time-sequence curve sequence, and identify the similar ion test concentration corresponding to the similar fluorescence time-sequence curve; Obtain the optimal probe preliminary test concentration of the similar ion test concentration, and perform fluorescence detection on the current ion solution to be measured according to the optimal probe preliminary test concentration.

[0083] Specifically, the specific implementation method of the processor 10 for the above instructions can refer to Figures 1 to 3 The descriptions of the relevant steps in the corresponding embodiments, which will not be elaborated herein.

[0084] Furthermore, if the modules / units integrated in the electronic device 1 are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer-readable storage medium. The computer-readable storage medium can be volatile or non-volatile. For example, the computer-readable medium can include: any entity or device capable of carrying the computer program code, recording medium, USB flash drive, mobile hard disk, magnetic disk, optical disc, computer memory, read-only memory (ROM, Read-Only Memory).

[0085] The present invention also provides a computer-readable storage medium. The readable storage medium stores a computer program, and when the computer program is executed by a processor of an electronic device, the following can be implemented: Obtain a probe pre-test gradient concentration sequence and an ion test gradient concentration sequence, and sequentially extract probe pre-test gradient concentrations from the probe pre-test gradient concentration sequence, where the probe pre-test gradient concentration sequence refers to a sequence composed of probe gradient concentrations for pre-testing a heavy metal ion solution; Obtain a pre-test gradient volume ratio sequence, and perform permutation and combination according to the pre-test gradient volume ratio sequence and the probe pre-test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; Sequentially extract probe concentration-volume ratio combinations from the probe concentration-volume ratio combination sequence; Perform a fluorescence intensity change test according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence time series curve sequence corresponding to the ion test gradient concentration sequence; Calculate the curve distribution uniformity coefficient of the fluorescence time series curve sequence to obtain a curve distribution uniformity coefficient set; Extract the minimum curve distribution uniformity coefficient from the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence time series curve sequence corresponding to the minimum curve distribution uniformity coefficient; Obtain a current ion solution to be measured, and use the target probe concentration-volume ratio combination to detect the fluorescence intensity change of the current ion solution to be measured to obtain a current fluorescence time series curve; Identify a similar fluorescence time series curve of the current fluorescence time series curve in the target fluorescence time series curve sequence, and identify the similar ion test concentration corresponding to the similar fluorescence time series curve; Obtain the optimal probe pre-test concentration of the similar ion test concentration, and perform fluorescence detection on the current ion solution to be measured according to the optimal probe pre-test concentration.

[0086] In several embodiments provided by the present invention, it should be understood that the disclosed devices, systems and methods can be implemented in other ways. For example, the system embodiments described above are only illustrative, and there may be other division methods in actual implementation.

[0087] The modules described as separate components may or may not be physically separated. The components shown as modules may or may not be physical units, that is, they may be located in one place, or may be distributed to multiple network units. Some or all of the modules can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0088] In addition, in each embodiment of the present invention, each functional module can be integrated in a processing unit, or each unit can exist physically alone, or two or more units can be integrated in one unit. The above integrated unit can be implemented in the form of hardware, or in the form of a combination of hardware and software functional modules.

[0089] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A fluorescence detection method for heavy metal ion concentration, characterized in that: The method comprises: Acquire a probe pre-test gradient concentration sequence and an ion test gradient concentration sequence, and extract probe pre-test gradient concentrations in the probe pre-test gradient concentration sequence in sequence, wherein the probe pre-test gradient concentration sequence refers to a sequence of probe gradient concentrations used for pre-testing a heavy metal ion solution; Obtaining a pre-test gradient volume ratio sequence, and performing permutations and combinations according to the pre-test gradient volume ratio sequence and the probe pre-test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; extracting probe concentration-volume ratio combinations in sequence from the probe concentration-volume ratio combination sequence; According to the probe concentration-volume ratio combination and the ion test gradient concentration sequence, a fluorescence intensity change test is performed to obtain a fluorescence time series curve sequence corresponding to the ion test gradient concentration sequence; Calculate the curve distribution uniformity coefficient of the fluorescence time series curve sequence to obtain a curve distribution uniformity coefficient set; Extract the minimum curve distribution uniformity coefficient from the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence time series curve sequence corresponding to the minimum curve distribution uniformity coefficient; Obtaining a current ion solution to be tested, and using the target probe concentration-volume ratio combination to detect a change in fluorescence intensity of the current ion solution to be tested, to obtain a current fluorescence timing curve; Identify a similar fluorescence timing curve of the current fluorescence timing curve in the target fluorescence timing curve sequence, and identify a similar ion test concentration corresponding to the similar fluorescence timing curve; The optimal probe pre-test concentration of similar ion test concentration is obtained, and fluorescence detection is performed on the current ion solution to be tested according to the optimal probe pre-test concentration.

2. The fluorescence detection method for heavy metal ion concentration according to claim 1, characterized in that: The step of obtaining the probe pre-test gradient concentration sequence and the ion test gradient concentration sequence comprises: The probe pre-test concentration threshold and probe pre-test refinement level input by the user are received, and the probe pre-test concentration gradient is calculated according to the probe pre-test concentration threshold using the following formula, wherein the smaller the probe pre-test refinement level, the higher the refinement of the pre-test of the heavy metal ion solution: ； in, represents the probe pre-test concentration gradient, represents the probe pre-test concentration threshold, represents a natural constant, Indicates the probe pre-test refinement level; Setting a probe pre-test gradient concentration sequence according to the probe pre-test concentration gradient; Obtain heavy metal ion concentration threshold and ion test fine level; According to the heavy metal ion concentration threshold and the ion test fineness level, the ion test concentration gradient is calculated using the following formula, wherein the smaller the ion test fineness level is, the higher the fineness of the pre-test of the heavy metal ion solution is: ； in, represents the ion test concentration gradient, represents the heavy metal ion concentration threshold, Indicates the fineness level of ion test; An ion test gradient concentration sequence is set according to the ion test concentration gradient.

3. The fluorescence detection method for heavy metal ion concentration according to claim 2, characterized in that: The method of arranging and combining the pre-test gradient volume ratio sequence and the probe pre-test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence includes: sequentially extracting pre-test volume ratios in the pre-test gradient volume ratio sequence, According to the pre-test volume ratio and each probe pre-test gradient concentration in the probe pre-test gradient concentration sequence, a probe concentration-volume ratio combination sequence is obtained, wherein the number of combinations of probe concentration-volume ratio combinations in the probe concentration-volume ratio combination sequence is equal to ,in, represents the number of pre-test gradient volume ratios in the pre-test gradient volume ratio sequence, Indicates the number of probe pre-test gradient concentrations in the probe pre-test gradient concentration sequence.

4. The fluorescence detection method for heavy metal ion concentration according to claim 3, characterized in that: The fluorescence intensity change test is performed according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence time series curve sequence corresponding to the ion test gradient concentration sequence, including: Extracting ion test gradient concentrations in the ion test gradient concentration sequence in sequence, and configuring a unit volume of ion test gradient solution according to the ion test gradient concentrations, wherein the unit volume is 100 mL; The pre-test volume ratio in the probe concentration-volume ratio combination is identified, and the probe pre-test solution volume is calculated according to the pre-test volume ratio and the unit volume using the following formula: ； in, represents the volume of the probe pre-test solution, It indicates the pre-test volume ratio; identifying a probe pre-test test concentration in the probe concentration-volume ratio combination, and configuring a probe pre-test test solution according to the probe pre-test test concentration and the probe pre-test solution volume; adding the probe pre-test solution to the ion test gradient solution to obtain a probe ion mixed solution; Performing real-time monitoring of the fluorescence intensity of the probe ion mixed solution to obtain a fluorescence timing curve, wherein the fluorescence timing curve refers to a curve of the fluorescence intensity of the probe ion mixed solution changing with time within a predetermined time; The fluorescence timing curves corresponding to the gradient concentrations of each ion test are collected to obtain a sequence of fluorescence timing curves.

5. The fluorescence detection method for heavy metal ion concentration according to claim 4, characterized in that: The step of calculating the curve distribution uniformity coefficient of the fluorescence time series curve sequence to obtain a curve distribution uniformity coefficient set includes: Acquire a fluorescence value time series, and time-mark each fluorescence time series curve in the fluorescence time series curve sequence according to the fluorescence value time series to obtain multiple groups of fluorescence marking point sequences, wherein a group of fluorescence marking point sequences refers to a sequence of fluorescence marking points obtained after time marking on a fluorescence time series curve; Identifying the fluorescence intensity slope corresponding to each fluorescent marker point in the fluorescent marker point sequence to obtain a fluorescence intensity slope sequence; Calculating the slope change value of each fluorescent marker point in the fluorescent marker point sequence using a pre-constructed slope change formula to obtain a slope change value set; Identifying a maximum slope change value in the slope change value set, identifying an intermediate fluorescent marker point corresponding to the maximum slope change value, and identifying an intermediate fluorescence intensity slope corresponding to the intermediate fluorescent marker point; Classifying the fluorescent marker point sequence according to the intermediate fluorescent marker point to obtain a leading fluorescent marker point sequence and a trailing fluorescent marker point sequence, wherein the leading fluorescent marker point sequence refers to a sequence of fluorescent marker points located in front of the intermediate fluorescent marker point in the fluorescent marker point sequence, and the trailing fluorescent marker point refers to a sequence of fluorescent marker points located in back of the intermediate fluorescent marker point in the fluorescent marker point sequence; Collecting the pre-fluorescence marking point sequences and post-fluorescence marking point sequences corresponding to each fluorescence time series curve to obtain multiple groups of pre-fluorescence marking point sequences and multiple groups of post-fluorescence marking point sequences; According to the plurality of groups of pre-fluorescent marker point sequences, the pre-distribution uniformity coefficient is calculated using the following formula: ； in, represents the pre-distribution uniformity coefficient, represents the pre-weight, Indicates the number of groups of the preceding fluorescent marker sequence or the number of fluorescence timing curves in the fluorescence timing curve sequence. represents the fluorescence intensity of the first pre-fluorescent marker point in the p+1th group of pre-fluorescent marker point sequence, represents the fluorescence intensity of the first pre-fluorescent marker point in the p-th group of pre-fluorescent marker point sequences, represents the fluorescence intensity of the first pre-fluorescent marker point in the Pth group of pre-fluorescent marker point sequences, represents the fluorescence intensity of the first pre-fluorescent marker point in the first group of pre-fluorescent marker point sequences, Indicates the first in the p+1th group of pre-fluorescent marker sequence The fluorescence intensity of the pre-fluorescent marker point, Indicates the first group of the p-th group of pre-fluorescent marker sequence The fluorescence intensity of the pre-fluorescent marker point, Indicates the first group of pre-fluorescent marker sequence The fluorescence intensity of the pre-fluorescent marker point, Indicates the first fluorescent marker sequence in the first group P The fluorescence intensity of the pre-fluorescent marker point, Indicates the total number of pre-fluorescent marker points in the pre-fluorescent marker point sequence; According to the multiple groups of post-fluorescent marker point sequences, the post-distribution uniformity coefficient is calculated using the following formula: ； in, represents the post-distribution uniformity coefficient, represents the post-weight, Indicates the number of groups of post-fluorescence marker point sequences or the number of fluorescence timing curves in the fluorescence timing curve sequence. represents the fluorescence intensity of the first post-fluorescent marker point in the p+1th group of post-fluorescent marker point sequence, represents the fluorescence intensity of the first post-fluorescent marker point in the pth group of post-fluorescent marker point sequence, represents the fluorescence intensity of the first post-fluorescent marker point in the Pth group of post-fluorescent marker point sequence, represents the fluorescence intensity of the first post-fluorescence marker point in the first group of post-fluorescence marker point sequences, Indicates the first in the p+1th group of post-fluorescent marker sequence The fluorescence intensity of the post-fluorescence marker point, Indicates the pth group of post-fluorescent marker sequence The fluorescence intensity of the post-fluorescence marker point, Indicates the first group of post-fluorescent marker sequence The fluorescence intensity of the post-fluorescence marker point, Indicates the first post-fluorescent marker sequence of group P The fluorescence intensity of the post-fluorescence marker point, Represents the total number of post-fluorescent marker points in the post-fluorescent marker point sequence; According to the pre-distribution uniformity coefficient and the post-distribution uniformity coefficient, the curve distribution uniformity coefficient is calculated using the following formula: ； in, It represents the coefficient of uniformity of curve distribution; The curve distribution uniformity coefficients of each probe concentration-volume ratio combination are collected to obtain a curve distribution uniformity coefficient set.

6. The fluorescence detection method for heavy metal ion concentration according to claim 5, characterized in that: The slope change formula is as follows: ； in, Indicates the slope change value of the first fluorescent marker point in the fluorescent marker point sequence. Represents the fluorescence intensity slope of the first fluorescent marker point in the sequence of fluorescent marker points. Represents the fluorescence intensity slope of the second fluorescent marker point in the sequence of fluorescent marker points. represents the slope change value of the i-th fluorescent marker point in the fluorescent marker point sequence, represents the fluorescence intensity slope of the i-1th fluorescent marker point in the sequence of fluorescent marker points, represents the fluorescence intensity slope of the i-th fluorescent marker point in the sequence of fluorescent marker points, represents the fluorescence intensity slope of the i+1th fluorescent marker point in the sequence of fluorescent marker points, Represents the total number of fluorescent markers in the sequence of fluorescent markers. Indicates the slope change value of the Ith fluorescent marker point in the fluorescent marker point sequence, represents the fluorescence intensity slope of the I-1th fluorescent marker point in the sequence of fluorescent marker points, represents the fluorescence intensity slope of the first fluorescent marker point in the sequence of fluorescent marker points, Represents the absolute value symbol.

7. The fluorescence detection method for heavy metal ion concentration according to claim 6, characterized in that: The step of identifying a similar fluorescence timing curve of the current fluorescence timing curve in a target fluorescence timing curve sequence includes: Time-marking the current fluorescence timing curve according to the fluorescence value time series to obtain a current fluorescence marking point sequence, wherein the fluorescence intensity change monitoring time of the current fluorescence timing curve is the predetermined time; Extracting target fluorescence timing curves in sequence from the target fluorescence timing curve sequence, calculating curve difference values ​​between the target fluorescence timing curve and the current fluorescence timing curve using a pre-constructed curve difference formula, and obtaining a curve difference value set; A minimum curve difference value is identified in the curve difference value set, and a similar fluorescence timing curve corresponding to the minimum curve difference value is identified in the target fluorescence timing curve sequence.

8. The fluorescence detection method for heavy metal ion concentration according to claim 7, characterized in that: The curve difference formula is as follows: ； in, represents the curve difference value, represents the fluorescence intensity of the jth current fluorescent marker point in the current fluorescent marker point sequence, Represents the fluorescence intensity of the jth fluorescent marker point in the target fluorescence time series curve.

9. The fluorescence detection method for heavy metal ion concentration according to claim 8, characterized in that: The method of obtaining the optimal probe pre-test concentration of similar ion test concentration comprises: According to the pre-constructed probe concentration formula, the optimal probe pre-test concentration is calculated using the similar ion test concentration, wherein the probe concentration formula is as follows: ； in, represents the optimal probe pre-test concentration, represents the ion ratio coefficient, Indicates similar ion test concentration.

10. A fluorescence detection system for heavy metal ion concentration, characterized in that: The system comprises: A probe concentration-volume ratio combination extraction module is used to obtain a probe pre-test gradient concentration sequence and an ion test gradient concentration sequence, and extract probe pre-test gradient concentrations in the probe pre-test gradient concentration sequence in sequence, wherein the probe pre-test gradient concentration sequence refers to a sequence of probe gradient concentrations used for pre-testing a heavy metal ion solution; obtain a pre-test gradient volume ratio sequence, and perform permutations and combinations according to the pre-test gradient volume ratio sequence and the probe pre-test gradient concentration sequence to obtain a probe concentration-volume ratio combination sequence; and extract probe concentration-volume ratio combinations in the probe concentration-volume ratio combination sequence in sequence; The target probe concentration-volume ratio combination identification module is used to perform a fluorescence intensity change test according to the probe concentration-volume ratio combination and the ion test gradient concentration sequence to obtain a fluorescence timing curve sequence corresponding to the ion test gradient concentration sequence; calculate the curve distribution uniformity coefficient of the fluorescence timing curve sequence to obtain a curve distribution uniformity coefficient set; extract the minimum curve distribution uniformity coefficient from the curve distribution uniformity coefficient set, and identify the target probe concentration-volume ratio combination and the target fluorescence timing curve sequence corresponding to the minimum curve distribution uniformity coefficient; A current fluorescence timing curve detection module is used to obtain the current ion solution to be tested, and use the target probe concentration-volume ratio combination to perform fluorescence intensity change detection on the current ion solution to be tested to obtain the current fluorescence timing curve; The fluorescence detection module of the current ion solution to be tested is used to identify the similar fluorescence timing curve of the current fluorescence timing curve in the target fluorescence timing curve sequence, identify the similar ion test concentration corresponding to the similar fluorescence timing curve; obtain the optimal probe pre-test concentration of the similar ion test concentration, and perform fluorescence detection on the current ion solution to be tested according to the optimal probe pre-test concentration.

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