Light reflection assembly for extended optical path measurement of microwell plate detector

Abstract

The invention relates to a divisional application of which the application number is 201911198047.2. The embodiment of the invention discloses a light reflection assembly for prolonging optical path measurement of a microwell plate detector. The light reflection assembly comprises a first reflection part and a second reflection part which are oppositely matched with each other, and the first reflection part and the second reflection part are each provided with a plurality of reflection mirror face sets. The first reflecting part and the second reflecting part are respectively fixed in an optical path detection system of a microwell plate detector and are positioned on two sides of a microwell plate solution, and optical detection of solutions in different micropores in the microwell plate is realized through movement of the microwell plate. Through the reflection of the reflection mirror group between the two reflection parts, the optical path of the initial incident light in the system to be measured is increased. The method has the beneficial effects that 1, under the condition of not changing the optical path size and appearance of the existing optical measurement instrument equipment, the measurement optical path of a system to be measured is increased, the detection limit of the existing method is reduced, and the sensitivity is improved; 2, due to the reflection effect between the two reflection parts, the light incident direction and the light emergent direction are both perpendicular to a system to be measured, and an existing optical measuring instrument can be matched with the light reflection assembly through simple light path adjustment.

Description

Division Case Explanation

[0001] This application is a divisional application. The application number of the original application is 201911198047.2, the application date is November 29, 2019, and the invention title is "An Optical Reflection Component for Extending Optical Path". Technical Field

[0002] The present invention belongs to the field of optical measurement, and specifically relates to an optical reflection component for extending the optical path measurement of a microplate detector. Background Art

[0003] Lambert-Beer's law is the basis of many optical analysis methods. Its quantitative analysis basis is that the measured absorbance A is proportional to the concentration c of the absorbing substance and the thickness b (optical path) of the absorption layer. Analysis methods based on this law have wide applications in fields such as environment, biosanitation, and water conservancy, and have the characteristics of low cost of instrument equipment, relatively simple operation, high result accuracy, and good reproducibility.

[0004] The optical path size has an important influence on the sensitivity and detection limit of the analysis method based on Lambert-Beer's law. The optical path of the common traditional colorimetric cuvette or flow-through optical detection cell for spectrophotometric analysis is mostly fixed in the range of 1 to 3 centimeters. Samples with small extinction coefficients or low concentrations are often difficult to measure, which greatly limits the range of substances and concentrations that can be detected by traditional spectrophotometric analysis.

[0005] A microplate detector, also commonly known as an enzyme-linked immunosorbent assay (ELISA) reader, is a conventional instrument for measuring enzyme-linked immunosorbent assays and is a variant of a professional spectrophotometer. The biggest difference between it and traditional spectrophotometric analysis is mainly that the volume of the absorption cell is small (in the microliter order of magnitude) and the optical path direction is perpendicular to the liquid surface of the solution. This makes the optical path length of the microplate detector lower than that of traditional spectrophotometric analysis, which also severely limits the application range of photometric analysis of the microplate detector. In the measurement of an immersion type full-spectrum analyzer, due to the fixed optical path length of the instrument, for the case where the concentration of the analyte in the water sample to be measured is low, at present, only by increasing the optical path length of the instrument can it be achieved, which greatly increases the manufacturing cost and weight of the instrument equipment and reduces the portability and universality range of the instrument. Gas optical detection also faces the problem of limited optical path length. At present, most of them can only partially solve the problem through calibration with high-concentration standard gases and long-distance detection. Summary of the Invention

[0006] In order to solve the above problems, the purpose of the present invention is to provide an optical reflection component for extending the optical path measurement of a microplate detector, which can effectively increase the optical path of light in the system to be measured, thereby reducing the detection limit of the system to be measured and improving the detection sensitivity, and has good practicability.

[0007] The present invention provides the following technical solutions:

[0008] An optical reflection component for extending the optical path measurement of a microplate detector, characterized in that the optical reflection component comprises a first reflection part and a second reflection part, and the first reflection part and the second reflection part are respectively arranged on both sides of the light-transmitting surface of the system to be measured; the first reflection part comprises m groups of first reflection mirror groups, and the second reflection part comprises n groups of second reflection mirror groups, where m is an integer greater than or equal to 1, and n is an integer greater than or equal to 0; each of the first reflection mirror groups and the second reflection mirror groups is formed by two reflection mirrors intersecting at a 90-degree angle, and the 90-degree angle formed by the two reflection mirrors faces the system to be measured, and the angle bisecting plane is perpendicular to the light-transmitting surface of the system to be measured.

[0009] The first reflection mirror groups of the first reflection part and the second reflection mirror groups of the second reflection part are arranged in a relative dislocation manner, so that the initial incident light travels forward in turn under the reflection of the first reflection mirror groups and the second reflection mirror groups.

[0010] As a further technical solution of the present invention, the optical reflection component is provided with a light incident hole / slit and a light exit hole / slit, and both the light incident hole / slit and the light exit hole / slit are arranged on the second reflection part or are respectively arranged on the second reflection part and the first reflection part.

[0011] The first reflection part 1 and the second reflection part 2 of the optical reflection component are respectively fixed in the optical path detection system of the microplate detector, on both sides of the microplate solution, and through the movement of the microplate, the optical detection of the solutions in different microholes in the microplate is realized.

[0012] As a further technical solution of the present invention, the first reflection mirror groups on the first reflection part and the second reflection mirror groups on the second reflection part can be rotated by different angles around their corresponding incident lights on a plane parallel to the light-transmitting surface of the system to be measured.

[0013] As a preferred technical solution of the present invention, the initial incident light source of the optical reflection component is a point light source or a line light source.

[0014] As a further technical solution of the present invention, the optical path length depends on the vertical width of the system to be measured between the first reflection part and the second reflection part and the number of reflections of the light in the system to be measured.

[0015] As a preferred technical solution of the present invention, the optical reflection component can be used either singly or in multiple sets and be built-in and positioned in the instrument optical path system, or can be freely placed outside the container of the system to be measured.

[0016] As a further technical solution of the present invention, the multiple sets of optical reflection components built-in and positioned in the instrument optical path detection system can be selected and exchanged manually or automatically to meet different optical path requirements.

[0017] The beneficial effects of the present invention are as follows:

[0018] 1. By arranging a first reflection part and a second reflection part that cooperate with each other on both sides of the light-transmitting surface of the system to be measured, when light enters the system to be measured, the measurement optical path is effectively increased through multiple reflections. Without changing the appearance and size of the existing optical measurement instrument, the detection limit of the existing method is reduced, and the detection sensitivity is improved.

[0019] 2. The reflection mirror group arranged on the first reflection part and the second reflection part makes the directions of the incident light and the outgoing light perpendicular to the system to be measured. The existing optical measurement instrument can be matched with this light reflection component by slightly adjusting the optical path system.

[0020] 3. The present invention increases the measurement optical path by arranging a reflection mirror group that cooperates with each other to increase the optical path through light reflection. The reflection mirror group does not directly contact the system to be measured, reducing the possibility of mirror damage and having strong practicability. Description of the Drawings

[0021] Figure 1a and 1b are the structural diagrams of the light emission component of the present invention for detecting a traditional colorimetric cuvette;

[0022] Figure 2a and 2b are the structural diagrams of the light emission component of the present invention for flow-through optical detection;

[0023] Figure 3 are the structural diagrams of the light emission component of the present invention for detecting a microplate reader;

[0024] Figure 4a and 4b are the structural diagrams of the light emission component of the present invention for detecting an immersion-type full-spectrum spectrometer;

[0025] Figure 5a and 5b are the structural diagrams of the light emission component of the present invention for gas optical detection;

[0026] Figure 6a and 6b are the schematic diagrams of the light path directions in two combined structural reflection mirror groups;

[0027] Figure 7 is the schematic diagram of the light path of the light emission component of the present invention for a rectangular light-transmitting surface. Among them, the center point of ⊙ is the light incident point, the center point of X is the light outgoing point, L i is the initial light incident position point, L e is the final light outgoing position point, and the dashed line represents the front and back order of the incident and outgoing light;

[0028] Figure 8 Schematic diagram of the optical path of the light-emitting component of the present invention for a circular light-transmitting surface. Among them, the center point of ⊙ is the light incident point, and the center point of X is the light exit point, L i is the initial light incident position point, L e is the final light exit position point, and the dotted line represents the front and back order of the incident and exit light;

[0029] The meanings of the marks in the attached drawings are as follows:

[0030] 1. First reflection part; 2. Second reflection part; 3. First reflection mirror group; 4. Second reflection mirror group; 5. Hole / slit for light to enter; 6. Hole / slit for light to exit; Specific implementation mode

[0031] The present invention will be specifically described below in conjunction with specific embodiments.

[0032] Embodiment 1

[0033] As Figure 1a and 1b shown, a colorimetric cell light reflection component with a longer optical path includes a first reflection part 1, a second reflection part 2, a light incident hole / slit 5 and a light exit hole / slit 6. Among them, several mutually corresponding first reflection mirror groups 3 are arranged on the first reflection part 1, and several mutually corresponding second reflection mirror groups 4 are arranged on the second reflection part 2.

[0034] As Figure 1a shown, the number of the first reflection mirror groups 3 on the first reflection part 1 of the colorimetric cell light reflection component is 1 more than the number of the second reflection mirror groups 4 arranged on the second reflection part 2. After the light enters through the light incident hole / slit 5 on the second reflection part 2, it is reflected by the first reflection mirror groups 3 on the first reflection part 1 and then reflected onto the second reflection mirror groups 4 on the second reflection part 2, and continues to be reflected in sequence. Finally, the light exits through the light exit hole / slit 6 located on the second reflection part 2. At this time, the initial incident light and the final exit light are on the same side of the solution.

[0035] As Figure 1b shown, the number of the first reflection mirror groups 3 on the first reflection part 1 of the colorimetric cell light reflection component is the same as the number of the second reflection mirror groups 4 arranged on the second reflection part 2. After the light enters through the light incident hole / slit 5 on the second reflection part 2, it is reflected by the first reflection mirror groups 3 on the first reflection part 1 and then reflected onto the second reflection mirror groups 4 on the second reflection part 2, and continues to be reflected in sequence. Finally, the light exits through the light exit hole / slit 6 located on the first reflection part 1. The initial incident light and the final exit light are on the opposite sides of the solution.

[0036] In this example, the cuvette light reflection component can be fixedly built into the optical path detection system, or can be designed as an external type such as in the form of a cuvette holder.

[0037] Example 2

[0038] As Figure 2a 、 2b As shown, a flow-through cuvette cell and a light emission component for a flow-through optical detection system. The light emission component includes a first reflection part 1, a second reflection part 2, a light incident hole / slit 5, and a light exit hole / slit 6. A plurality of mutually corresponding first reflection mirror groups 3 are arranged on the first reflection part 1, and a plurality of mutually corresponding second reflection mirror groups 4 are arranged on the second reflection part 2.

[0039] As Figure 2a As shown, a flow-through cuvette cell for a flow-through optical detection system has an outlet and an inlet that penetrate the system to be measured, and the cross-section of the cuvette cell is rectangular. The first reflection part 1 and the second reflection part 2 of the light reflection component are respectively arranged on both sides of the light-transmitting cell wall of the cross-section to be measured of the flow-through cuvette cell. The number of the first reflection mirror groups 3 on the first reflection part 1 of the cuvette cell light reflection component is the same as the number of the second reflection mirror groups 4 arranged on the second reflection part 2. After the point light source is incident from the light incident hole / slit 5 on the second reflection part 2, it is reflected by the first reflection mirror groups 3 on the first reflection part 1 to the second reflection mirror groups 4 on the second reflection part 2, and is continuously reflected in turn. Finally, the light exits from the light exit hole / slit 6 located on the first reflection part 1, and the initial incident light and the final exit light are located on both sides of the solution to be measured.

[0040] As Figure 2b As shown, different from the light emission component for flow-through optical detection shown in Figure 2a the number of the first reflection mirror groups 3 on the first reflection part 1 of this cuvette light reflection component is one more than the number of the second reflection mirror groups 4 arranged on the second reflection part 2. Both the light incident hole / slit 5 and the light exit hole / slit 6 are on the second reflection part 2, and the initial incident light and the final exit light are located on the same side of the solution.

[0041] The light emission component of the present invention can be built into the optical path detection system in a single set or multiple sets. When multiple sets are built into the optical path detection system, different optical path lengths can be achieved by rotating the light reflection components corresponding to different pairs. The cross-section of the flow-through cuvette cell can be square, circular, or other shapes, which can be adjusted by those skilled in the art according to actual needs.

[0042] When the system to be measured flows in the flow-through cuvette cell, the concentration will change along the liquid flow direction. In the present invention, a first reflecting mirror group 3 and a second reflecting mirror group 4 which are correspondingly distributed are respectively arranged on both sides of the light-transmitting wall of the cuvette cell. When light enters the flow-through cuvette cell at a certain cross-section perpendicular to the solution flow direction and is reflected multiple times on the same cross-section, the change in concentration can be captured more sensitively, improving the detection sensitivity and accuracy.

[0043] Example 3

[0044] As Figure 3 shown, a light-emitting component for extending the optical path measurement of a microplate reader.

[0045] The first reflecting part 1 and the second reflecting part 2 of the light reflection component are respectively fixed in the optical path detection system of the microplate reader, on both sides of the microplate solution. By moving the microplate, the optical detection of the solutions in different micro-wells in the microplate is realized.

[0046] In this embodiment, the first reflecting mirror group 3 and the second reflecting mirror group 4 which are correspondingly distributed are respectively arranged on the first reflecting part 1 and the second reflecting part 2, so that light can enter along the direction perpendicular to the solution in the micro-well and then exit along the direction perpendicular to the solution in the micro-well.

[0047] The light reflection component can be single set or multiple sets built into the optical path detection system of the microplate reader. By rotating the light reflection components corresponding to different pairs, different optical path lengths can be realized.

[0048] Example 4

[0049] As Figure 4a and 4b shown, a light-emitting component for extending the optical path measurement of an immersion full-spectrum detector.

[0050] The first reflecting part 1 and the second reflecting part 2 of the light reflection component are respectively fixed in the optical path detection system of the immersion full-spectrum detector, on both sides of the water / aqueous solution to be measured and sealed in an optically transparent sealing window. Through the first reflecting mirror group 3 and the second reflecting mirror group 4 which are correspondingly distributed, light can enter perpendicular to the water / aqueous solution to be measured and then exit perpendicular to the water / aqueous solution.

[0051] The light reflection component can be single set or multiple sets built into the optical path detection system of the immersion full-spectrum detector. By rotating the sets of light reflection components corresponding to different pairs, different optical path lengths can be realized.

[0052] Example 5

[0053] As Figure 5a and 5bAs shown, an optical emission component for gas optical extended optical path detection.

[0054] The first reflection part 1 and the second reflection part 2 of the optical reflection component are respectively fixed in the optical path detection system of the gas optical detector, on both sides of the light-transmitting surface of the gas to be measured or its container / cell.

[0055] In this embodiment, first reflection mirror groups 3 and second reflection mirror groups 4 are respectively arranged on the first reflection part 1 and the second reflection part 2 in a correspondingly distributed manner, so that light can enter along a direction perpendicular to the gas to be measured and then exit along a direction perpendicular to the gas to be measured.

[0056] The optical reflection component can be a single set or multiple sets positioned in the gas optical detection optical path system. By rotating the correspondingly paired optical reflection components, different optical path lengths can be achieved.

[0057] Embodiment 6

[0058] Figure 6a and 6b are schematic diagrams of the light ray directions of two combined structures of the present invention; Figure 6a In, the reflection mirror group 3 and the reflection mirror group 4 are arranged in a staggered manner on both sides of the parallel light-transmitting surface, and the angular bisecting planes of the 90-degree angle between the two reflection mirror groups are parallel. At this time, light enters from the light incident hole / slit 5 on the second reflection part 2 or is incident by the light exiting from the previous mirror group. After two consecutive reflections on the reflection mirror group 3, it exits to the reflection mirror group 4, and then after two consecutive reflections on the reflection mirror group 4, it exits to the first reflection part 1. After multiple reflections like this, the incident light ray and the exiting light ray are on the same plane, increasing the optical path of the light ray in the system to be measured.

[0059] Figure 6b In, the reflection mirror group 3 and the reflection mirror group 4 are arranged in a staggered manner on both sides of the light-transmitting surface, and the angular bisecting planes of the 90-degree angle between the two reflection mirror groups are perpendicular to each other. At this time, light enters from the light incident hole / slit 5 on the second reflection part 2 or is incident by the light exiting from the previous mirror group. After two reflections on the reflection mirror group 3, it exits to the reflection mirror group 4, and then after two reflections on the reflection mirror group 4, it exits to the first reflection part 1. After consecutive reflections through this combined structure like this, the initial incident light ray and the final exiting light ray are on different planes, increasing the optical path of the light ray in the system to be measured and also converting the direction of the light ray path.

[0060] Furthermore, the first reflection mirror group 3 on the first reflection part 1 and the second reflection mirror group 4 on the second reflection part 2 can be arranged to rotate at different angles around their corresponding incident light as the axis on the parallel planes relative to the light-transmitting surface of the system to be measured, so as to change the path of the corresponding exiting light ray in the system to be measured, and further extend the optical path range of the initial incident light ray of the point light source in the system to be measured.

[0061] Example 7

[0062] Figure 7 The figure shows a schematic diagram of one of the optical path for the light-emitting component of the present invention for a rectangular light-transmitting surface, including a combination of a number of reflecting mirror groups as shown in Figure 6a and 6b The light enters at the position shown by Li and is successively reflected by a number of Figure 6a shown combined structures. At this time, the optical path is on a plane. The reflecting mirror group 4 is arranged in the structure shown in Figure 6b so that the light reflected by the reflecting mirror group 4 is perpendicular to the plane where the previous light is located. By such continuous combination, finally the light can move forward successively along the path trajectory shown by the dotted line in Figure 7

[0063] Furthermore, the reflecting mirror group 3 and the reflecting mirror group 4 can be set in an arbitrarily rotatable combination around their corresponding incident lights on a plane parallel to the light-transmitting surface of the system to be measured, and the required optical path size can be selected within the limited reflection space, so that the reflected optical path presents a broken line or arc trajectory. As Figure 8 shown is a schematic diagram of one of the spiral optical paths of the light-emitting component of the present invention on a circular light-transmitting surface. This setting can comprehensively consider the size of the light-transmitting surface of the system to be measured and maximize the optical path of the light in the system to be measured during detection.

[0064] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.​

Claims

1. A light reflection component for extended optical path measurement of a microplate detector, characterized in that, the light reflection component includes a first reflection part (1) and a second reflection part (2), and the first reflection part (1) and the second reflection part (2) are respectively arranged on both sides of the system to be measured; the first reflection part (1) includes m groups of first reflection mirror groups (3), and the second reflection part (2) includes n groups of second reflection mirror groups (4), where m is an integer greater than or equal to 1, and n is an integer greater than or equal to 0; the reflection mirror groups of the first reflection mirror group (3) and the second reflection mirror group (4) are each formed by two reflection mirrors intersecting at a 90-degree angle, and the 90-degree angle formed by the two reflection mirrors faces the system to be measured, and the angle bisecting plane is perpendicular to the system to be measured; the first reflection mirror group (3) of the first reflection part (1) and the second reflection mirror group (4) of the second reflection part (2) are arranged in relative dislocation so that the initial incident light travels successively under the reflection of the first reflection mirror group (3) and the second reflection mirror group (4); a light incident hole / slit (5) and a light exit hole / slit (6) are provided on the light reflection component, and both the light incident hole / slit (5) and the light exit hole / slit (6) are provided on the second reflection part (2) or respectively on the second reflection part (2) and the first reflection part (1); the first reflection part 1 and the second reflection part 2 of the light reflection component are respectively fixed in the optical path detection system of the microplate detector, located on both sides of the microplate solution, and through the movement of the microplate, the optical detection of the solutions in different microholes in the microplate is realized.

2. A light reflection component for extended optical path measurement of a microplate detector according to claim 1, characterized in that, the first reflection mirror group (3) on the first reflection part (1) and the second reflection mirror group (4) on the second reflection part (2) can rotate at different angles around their respective incident lights on a plane parallel to the light-transmitting surface of the system to be measured.

3. A light reflection component for extended optical path measurement of a microplate detector according to claim 1 or 2, characterized in that, the optical path length depends on the vertical width of the system to be measured between the first reflection part (1) and the second reflection part (2) and the number of reflections of the light in the system to be measured.

4. A light reflection component for extended optical path measurement of a microplate detector according to claim 1, characterized in that, the light reflection component can be used either singly or in multiple sets and be internally positioned in the instrument optical path system.

5. A light emission component for extended optical path measurement of a microplate detector according to claim 4, characterized in that, multiple sets of light reflection components internally positioned in the instrument optical path detection system can be switched and selected manually or automatically to meet different optical path requirements.

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