Preparation and application of whitening and repairing composition containing temperature-sensitive oxidized resveratrol hydrogel

Abstract

The invention discloses preparation and application of a whitening and repairing composition containing temperature-sensitive oxidized resveratrol hydrogel, and relates to the technical field of cosmetics. The composition comprises the following components in parts by weight: 1-5 parts of temperature-sensitive oxidized resveratrol hydrogel, 4-6 parts of a peucedanum praeruptorum dunn extract, 1-3 parts of a willowleaf swallowwort rhizome extract and 0.1-0.5 part of a tephrosia rubescens seed extract. The combination of the peucedanum praeruptorum dunn extract, the cynanchum wilfordii extract, the tephros glauca seed extract and the oxyresveratrol finds that the composition has a synergistic interaction effect, and the composition formed by the components through specific compatibility has the effects of whitening, repairing and improving skin elasticity. The oxidized resveratrol is wrapped by the temperature-sensitive hydrogel, so that the oxidized resveratrol is kept in a gel state at an epidermal cuticle layer, and after the oxidized resveratrol permeates into a skin basal layer, the oxidized resveratrol can be released at the skin basal layer, acts on melanocytes more effectively, inhibits tyrosinase and further affects generation of melanin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to the preparation and application of a whitening and repairing composition containing a thermosensitive resveratrol oxide hydrogel. Background Art

[0002] The color of the skin is mainly determined by melanin. Melanin is a bio-pigment that is insoluble in water and soluble in alkaline solutions and belongs to protein derivatives. Melanin is synthesized in melanosomes in melanocytes located in the basal layer of the skin, and then transferred to keratinocytes through the dendritic processes of melanocytes, and ascends to the stratum corneum along with epidermal cells, and finally excreted with the shedding of the stratum corneum. The synthesis of melanin in melanosomes is extremely complex, and tyrosinase is the key enzyme in this reaction. There are already a variety of whitening ingredients used in whitening products on the market. Among them, some are aimed at melanin production, such as arbutin, kojic acid, etc., and some are aimed at melanin transport, such as niacinamide, etc.

[0003] At present, most whitening products will add ingredients that inhibit tyrosinase and melanin production, and some will also be rationally compounded with ingredients that inhibit melanin transport, etc., to achieve the effect of full-link whitening. However, since the melanin production link is in the basal layer of the skin, the ingredients that inhibit melanin production will be degraded under the influence of light, etc. after the whitening product is applied, and the content penetrating into the basal layer will decrease, and its inhibitory effect on melanin cannot be efficiently exerted. The skin consists of the epidermis, dermis and subcutaneous tissue, and the skin temperature shows a gradient distribution. Research shows that the temperature of the deep layer of the skin is higher than that of the surface layer. When the ambient temperature is 25 °C, the skin surface temperature is 30 °C, while the bottom layer temperature reaches 37 °C. Therefore, most of the ingredients that inhibit melanin production in existing whitening products are directly added, and cannot meet the requirement of accurately acting on melanocytes by penetrating into the basal layer. Summary of the Invention

[0004] Based on this, the purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a preparation and application of a whitening and repairing composition containing a thermosensitive resveratrol oxide hydrogel, and this combination has the effects of whitening, repairing and improving skin elasticity.

[0005] To achieve the above purpose, the technical solutions adopted by the present invention are as follows:

[0006] In the first aspect, the present invention provides a whitening and repairing composition, and the composition includes the following components in parts by weight: 1-5 parts of thermosensitive resveratrol oxide hydrogel, 4-6 parts of Peucedanum praeruptorum Dunn extract, 1-3 parts of Premna serratifolia L. extract, and 0.1-0.5 part of Tephrosia purpurea (L.) Pers. seed extract.

[0007] Oxidized resveratrol is a stilbene compound, which has the effects of inhibiting tyrosinase activity and melanin production. Its inhibitory ability against tyrosinase is 32 times that of kojic acid. It is a potential tyrosinase inhibitor and has good whitening effects.

[0008] Due to its thermosensitive characteristics, the thermosensitive oxidized resveratrol hydrogel of the present invention has a thermal response temperature of 33.5 - 36°C. When the hydrogel is in the epidermal stratum corneum, since the temperature of the epidermal stratum corneum is lower than its thermal response temperature, the lactam group of the oxidized resveratrol hydrogel forms hydrogen bonds with water and will maintain a gel state; after it penetrates into the skin basal layer, since the temperature of the basal layer (35 - 36°C) is higher than its thermal response temperature (33.5 - 36°C), as the temperature rises to reach the thermal response temperature, the hydrogen bonds are broken, and hydrophobic interactions dominate. The three-dimensional network structure of the hydrogel expands, and oxidized resveratrol will be released in the basal layer, acting more deeply on melanocytes, inhibiting tyrosinase, and thus affecting melanin production, and its whitening effect is more significant.

[0009] The extract of Peucedanum praeruptorum Dunn contains the vitamin E derivative tocopheryl acetate, which has the ability to scavenge free radicals. In addition, the extract of Peucedanum praeruptorum Dunn also contains ceramide, which can repair the skin barrier. The extract of Potentilla discolor Bunge can soothe the skin, reduce skin inflammation and swelling, and at the same time has antioxidant function, which can delay skin aging. The extract of Tephrosia purpurea (L.) Pers. can improve the metabolism of the skin, quickly discharge melanin out of the skin. In addition, it can also stimulate the release of β-endorphin, enhance the pleasure of the skin, regulate skin stress at the neural level, and solve the problem of skin barrier damage caused by stress.

[0010] The present invention reasonably combines the above several components into a composition, so that the composition has comprehensive effects of whitening, repairing and improving skin elasticity.

[0011] As a preferred embodiment of the first aspect, the composition comprises the following components in parts by weight: 3 parts of thermosensitive oxidized resveratrol hydrogel, 5 parts of extract of Peucedanum praeruptorum Dunn, 2 parts of extract of Potentilla discolor Bunge, and 0.3 part of extract of Tephrosia purpurea (L.) Pers.

[0012] The present invention studies and finds that the parts by weight of the thermosensitive oxidized resveratrol hydrogel, extract of Peucedanum praeruptorum Dunn, extract of Potentilla discolor Bunge, and extract of Tephrosia purpurea (L.) Pers. in the whitening and repairing composition will affect the whitening, repairing and skin elasticity improving effects of the composition to a certain extent. When further selecting 3 parts of thermosensitive oxidized resveratrol hydrogel, 5 parts of extract of Peucedanum praeruptorum Dunn, 2 parts of extract of Potentilla discolor Bunge, and 0.3 part of extract of Tephrosia purpurea (L.) Pers., the overall comprehensive effect of the composition can be enhanced to the greatest extent.

[0013] As a preferred embodiment of the first aspect, the mass ratio of the Peucedanum praeruptorum Dunn extract to the Peucedanum praeruptorum Dunn extract is: Peucedanum praeruptorum Dunn extract: Peucedanum praeruptorum Dunn extract = (2.5 - 3): 1. For example, it can be, but is not limited to, 2.5: 1, 2.6: 1, 2.7: 1, 2.8: 1, 2.9: 1, 3: 1.

[0014] As a preferred embodiment of the first aspect, the mass ratio of the Peucedanum praeruptorum Dunn extract to the Peucedanum praeruptorum Dunn extract is: Peucedanum praeruptorum Dunn extract: Peucedanum praeruptorum Dunn extract = 3: 1.

[0015] The present invention's research found that the mass ratio of the Peucedanum praeruptorum Dunn extract to the Peucedanum praeruptorum Dunn extract in the composition will, to a certain extent, affect the whitening, repair, and skin elasticity enhancing effects of the composition. When further selecting the mass ratio of the Peucedanum praeruptorum Dunn extract to the Peucedanum praeruptorum Dunn extract to be (2.5 - 3): 1, it can enhance the whitening, repair, and skin elasticity enhancing effects of the composition. In particular, when the mass ratio of the Peucedanum praeruptorum Dunn extract to the Peucedanum praeruptorum Dunn extract is 3: 1, it can maximize the overall comprehensive effect of the composition.

[0016] As a preferred embodiment of the first aspect, the thermosensitive resveratrol oxide hydrogel comprises the following components in mass percentages: resveratrol oxide 1 - 3%, sodium alginate 0.5 - 1%, polyvinylcaprolactam 15 - 25%, ammonium persulfate 0.4 - 0.6%, butanediol 20 - 30%, and the balance is water.

[0017] As a preferred embodiment of the first aspect, the mass percentage of the resveratrol oxide can be any value or any range value among 1%, 1.2%, 1.4%, 1.5%, 1.6%, 1.8%, 1.9%, 2%, 2.2%, 2.4%, 2.5%, 2.6%, 2.8%, 2.9%, or 3%.

[0018] As a preferred embodiment of the first aspect, the mass percentage of the sodium alginate can be any value or any range value among 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, or 1%.

[0019] As a preferred embodiment of the first aspect, the mass percentage of the polyvinylcaprolactam can be any value or any range value among 15%, 17%, 19%, 20%, 22%, 24%, or 25%.

[0020] As a preferred embodiment of the first aspect, the mass percentage of the ammonium persulfate can be any value or any range value among 0.4%, 0.5%, or 0.6%.

[0021] As a preferred embodiment of the first aspect, the mass percentage of the butanediol can be any value or any range value among 20%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% or 30%.

[0022] As a preferred embodiment of the first aspect, the mass ratio of resveratrol oxide to polyvinylcaprolactam is: resveratrol oxide: polyvinylcaprolactam = 1:(9 - 11). For example, it can be but is not limited to 1:9, 1:10, 1:11.

[0023] As a preferred embodiment of the first aspect, the mass ratio of resveratrol oxide to polyvinylcaprolactam is: resveratrol oxide: polyvinylcaprolactam = 1:11.

[0024] As a preferred embodiment of the first aspect, the mass ratio of sodium alginate to polyvinylcaprolactam is: sodium alginate: polyvinylcaprolactam = 1:(22 - 28). For example, it can be but is not limited to 1:22, 1:23, 1:24, 1:25, 1:26, 1:27, 1:28.

[0025] As a preferred embodiment of the first aspect, the mass ratio of sodium alginate to polyvinylcaprolactam is: sodium alginate: polyvinylcaprolactam = 1:27.5.

[0026] As a preferred embodiment of the first aspect, the preparation method of the thermosensitive resveratrol oxide hydrogel comprises the following steps:

[0027] (1) Dissolve part of the sodium alginate in water;

[0028] (2) Dissolve the polyvinylcaprolactam in water at a temperature below 10°C, stir until transparent, and store at 4°C;

[0029] (3) Slowly add the polyvinylcaprolactam solution prepared in step (2) to the sodium alginate solution prepared in step (1), and stir;

[0030] (4) Add ammonium persulfate to the mixed solution in step (3), and continuously stir;

[0031] (5) Dissolve the resveratrol oxide in the butanediol, continuously stir, and after complete dissolution, add it to the mixed solution prepared in step (4), stir and then filter to obtain the thermosensitive hydrogel loaded with resveratrol oxide.

[0032] The present invention finds through research that the mass ratio of resveratrol oxide to polyvinylcaprolactam, and the mass ratio of sodium alginate to polyvinylcaprolactam in the whitening and repairing composition will affect the whitening, repairing and skin elasticity enhancing effects of the composition to a certain extent. When further selecting the mass ratio of resveratrol oxide to polyvinylcaprolactam as 1:(9 - 11), and the mass ratio of sodium alginate to polyvinylcaprolactam as 1:(22 - 28), the overall effect of the composition can be improved. Especially when the mass ratio of resveratrol oxide to polyvinylcaprolactam is 1:11, and the mass ratio of sodium alginate to polyvinylcaprolactam is 1:27.5, the overall comprehensive effect of the composition can be maximally enhanced.

[0033] As a preferred embodiment of the first aspect, the preparation method of the Peucedanum praeruptorum Dunn extract comprises the following steps: taking dried and pulverized Peucedanum praeruptorum Dunn, adding it into an ethanol solution, performing ultrasonic extraction, filtering, and concentrating the filtrate to obtain the Peucedanum praeruptorum Dunn extract.

[0034] As a preferred embodiment of the first aspect, the preparation method of the Peucedanum praeruptorum Dunn extract satisfies at least one of the following:

[0035] (a) The ethanol concentration in the ethanol solution is 60 - 70%;

[0036] (b) The power of the ultrasonic extraction is 100 - 120 W;

[0037] (c) The time of the ultrasonic extraction is 30 - 60 min.

[0038] As a preferred embodiment of the first aspect, the preparation method of the Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract comprises the following steps:

[0039] Taking dried and pulverized Cynanchum stauntonii (Decne.) Schltr. ex Levl., adding it into an aqueous solution of a deep eutectic solvent, performing ultrasonic extraction, then centrifuging and filtering, and collecting the filtrate to obtain the Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract; wherein, the deep eutectic solvent used is composed of betaine, propylene glycol and glycerol.

[0040] As a preferred embodiment of the first aspect, the Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract satisfies at least one of the following:

[0041] (a) The mass - volume ratio of betaine, propylene glycol and glycerol is: betaine:propylene glycol:glycerol = 9 - 11 g:2 - 4 mL:2 - 4 mL; preferably, the mass - volume ratio of betaine, propylene glycol and glycerol is: betaine:propylene glycol:glycerol = 10 g:3 mL:3 mL;

[0042] (b) The mass concentration of the deep eutectic solvent in the aqueous solution of the deep eutectic solvent is 10 - 20%;

[0043] (d) The power of the ultrasonic extraction is 60 - 70 W;

[0044] (e) The time for ultrasonic extraction is 30 - 60 min.

[0045] The present invention discovers that different extraction methods have a great influence on the extracts of Peucedanum praeruptorum Dunn and Premna serratifolia L., and affect the synergistic effect of the extracts of Peucedanum praeruptorum Dunn and Premna serratifolia L. with other components, as well as the whitening, repair and skin elasticity enhancing effects of the whitening and repair composition, thus affecting the overall comprehensive effect of the composition.

[0046] In the second aspect, the present invention provides a whitening product, and the whitening product includes the whitening and repair composition as described in the first aspect.

[0047] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0048] The present invention loads resveratrol oxide on a thermosensitive hydrogel, so that it remains in a gel state at the epidermal stratum corneum. After penetrating into the skin basal layer, since the temperature of the basal layer (35 - 36 °C) is higher than its thermal response temperature (33.5 - 36 °C), resveratrol oxide will be released at the basal layer and act more effectively on melanocytes, inhibiting tyrosinase and thus affecting the production of melanin. The present invention discovers from numerous plant extracts that the extracts of Peucedanum praeruptorum Dunn, Premna serratifolia L. and Tephrosia purpurea (L.) Pers. have a synergistic effect with resveratrol oxide, and the composition formed by their specific compatibility has comprehensive effects of whitening, repair and skin elasticity enhancing. Detailed implementation mode

[0049] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. The purpose is to understand the content of the present invention in detail, rather than a limitation to the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The experimental reagents and instruments designed in the embodiments and comparative examples of the present invention are all common ordinary reagents and instruments unless otherwise specified, and can be obtained from commercial channels. In the embodiments and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are of the same batch. Further description of the raw materials used in the present invention:

[0050] The extract of Tephrosia purpurea (L.) Pers. is purchased from Givaudan Corporation in Switzerland, product name: Neurophroline TM ;

[0051] The extract of Angelica sinensis (Oliv.) Diels is purchased from Xi'an Tianguangyuan Biotechnology Co., Ltd., product name: extract of Angelica sinensis (Oliv.) Diels;

[0052] The Cynanchum bungei Decne. extract was purchased from Baoji Kaiweikang Biotechnology Co., Ltd., and the product name is Cynanchum bungei Decne. extract;

[0053] The Glycine soja Sieb. et Zucc. seed extract was purchased from Guangzhou Nuoran Biotechnology Co., Ltd., and the product name is soybean extract.

[0054] The preparation method of thermosensitive resveratrol oxide hydrogels - 1 to 6 was carried out according to the ratio shown in Table 1 below:

[0055] (1) Dissolve part of sodium alginate in water;

[0056] (2) Dissolve polyvinylcaprolactam in water at a temperature below 10 °C, stir until transparent, and store at 4 °C;

[0057] (3) Slowly add the polyvinylcaprolactam solution prepared in step (2) to the sodium alginate solution prepared in step (1), and stir;

[0058] (4) Add ammonium persulfate to the mixed solution in step (3), and continuously stir;

[0059] (5) Dissolve resveratrol oxide in butanediol, continuously stir, and after complete dissolution, add it to the mixed solution prepared in step (4), stir and then filter to obtain the thermosensitive hydrogel loaded with resveratrol oxide.

[0060] Table 1: Mass percentage (%) of each component in the hydrogel

[0061]

[0062] The only difference between thermosensitive resveratrol oxide hydrogel - 5 and thermosensitive resveratrol oxide hydrogel - 4 is that chitosan is used to replace sodium alginate.

[0063] The only difference between thermosensitive resveratrol oxide hydrogel - 6 and thermosensitive resveratrol oxide hydrogel - 4 is that polyisopropylacrylamide is used to replace polyvinylcaprolactam.

[0064] The preparation method of resveratrol oxide hydrogel - 7 (non-thermosensitive hydrogel):

[0065] (1) Dissolve part of sodium alginate in water;

[0066] (2) Dissolve acrylamide in part of water, stir and mix evenly, add ammonium persulfate, seal, and stir at 90 °C for 2 h to obtain polyacrylamide hydrogel;

[0067] (3) Add the polyacrylamide solution prepared in step (2) to the sodium alginate solution prepared in step (1), and stir;

[0068] (4) Dissolve resveratrol oxide in butanediol, stir continuously. After complete dissolution, add it to the mixed solution prepared in step (3), stir, filter, let it stand, and store at room temperature to obtain resveratrol oxide hydrogel-7.

[0069] The mass percentages of each component in resveratrol oxide hydrogel-7 are as follows: 2% resveratrol oxide, 0.8% sodium alginate, 5% polyacrylamide, 0.15% ammonium persulfate, 25% butanediol, and the balance is water.

[0070] The preparation method of Peucedanum praeruptorum Dunn extract-1 is as follows:

[0071] Take commercially available Peucedanum praeruptorum Dunn, dry it, pulverize it and pass through a 50-mesh sieve. Add 10 times the amount of 60% ethanol, and perform ultrasonic treatment for 40 min under the condition of an ultrasonic power of 110 W and an ultrasonic temperature of 40 °C. Filter and collect the filtrate; use a rotary evaporator to concentrate the filtrate to 1 / 3 of the original solution, and then add water to the original volume to obtain Peucedanum praeruptorum Dunn extract-1.

[0072] The preparation method of Peucedanum praeruptorum Dunn extract-2 is as follows:

[0073] Take commercially available Peucedanum praeruptorum Dunn, dry it, pulverize it and pass through a 40-mesh sieve. Add 9 times the amount of 65% ethanol, and perform ultrasonic treatment for 60 min under the condition of an ultrasonic power of 100 W and an ultrasonic temperature of 55 °C. Filter and collect the filtrate; use a rotary evaporator to concentrate the filtrate to 1 / 4 of the original solution, and then add water to the original volume to obtain Peucedanum praeruptorum Dunn extract-2.

[0074] The preparation method of Peucedanum praeruptorum Dunn extract-3 is as follows:

[0075] Take commercially available Peucedanum praeruptorum Dunn, dry it, pulverize it and pass through a 60-mesh sieve. Add 8 times the amount of 70% ethanol, and perform ultrasonic treatment for 30 min under the condition of an ultrasonic power of 120 W and an ultrasonic temperature of 60 °C. Filter and collect the filtrate; use a rotary evaporator to concentrate the filtrate to 1 / 3 of the original solution, and then add water to the original volume to obtain Peucedanum praeruptorum Dunn extract-3.

[0076] The preparation method of Peucedanum praeruptorum Dunn extract-4 is as follows:

[0077] Take commercially available Peucedanum praeruptorum Dunn, pulverize it and pass through a 50-mesh sieve. Add 10 times the amount of 30% deep eutectic solvent aqueous solution and mix. Stir at 50 °C for 60 min, and use ultrasonic wave for auxiliary extraction during this period, with an ultrasonic power of 60 W; the deep eutectic solvent used is composed of butanediol and malic acid, and the molar ratio of butanediol:malic acid = 1:4. Then filter and collect the filtrate to obtain Peucedanum praeruptorum Dunn extract-4.

[0078] The preparation method of Peucedanum praeruptorum Dunn extract-5 is as follows:

[0079] Take commercially available Peucedanum praeruptorum Dunn, crush it and sieve it through a 50-mesh sieve, add 10 times the amount of 30% deep eutectic solvent aqueous solution and mix, stir at 50 °C for 60 min, and use ultrasonic-assisted extraction during this period, with the ultrasonic power being 60 W; the deep eutectic solvent used is composed of betaine and glycerol, and their molar ratio is betaine:glycerol = 5:2. Then filter and collect the filtrate to obtain Peucedanum praeruptorum Dunn extract-5.

[0080] The preparation method of Patrinia villosa Juss. extract-1 is as follows:

[0081] Take commercially available Patrinia villosa Juss., crush it and sieve it through a 40-mesh sieve, add 25 times the amount of 15% deep eutectic solvent aqueous solution and mix, stir at 50 °C for 50 min, and use ultrasonic-assisted extraction during this period, with the ultrasonic power being 60 W, the ultrasonic time being 50 min, and the ultrasonic temperature being 50 °C; the deep eutectic solvent used is composed of betaine, propylene glycol and glycerol, and their mass-volume ratio is betaine:propylene glycol:glycerol = 10 g:3 mL:3 mL. Then centrifuge and filter, and collect the filtrate to obtain Patrinia villosa Juss. extract-1.

[0082] The preparation method of Patrinia villosa Juss. extract-2 is as follows:

[0083] Take commercially available Patrinia villosa Juss., crush it and sieve it through a 50-mesh sieve, add 20 times the amount of 10% deep eutectic solvent aqueous solution and mix, stir at 55 °C for 30 min, and use ultrasonic-assisted extraction during this period, with the ultrasonic power being 65 W, the ultrasonic time being 30 min, and the ultrasonic temperature being 55 °C; the deep eutectic solvent used is composed of betaine, propylene glycol and glycerol, and their mass-volume ratio is betaine:propylene glycol:glycerol = 9 g:4 mL:2 mL. Then centrifuge and filter, and collect the filtrate to obtain Patrinia villosa Juss. extract-2.

[0084] The preparation method of Patrinia villosa Juss. extract-3 is as follows:

[0085] Take commercially available Patrinia villosa Juss., crush it and sieve it through a 60-mesh sieve, add 30 times the amount of 20% deep eutectic solvent aqueous solution and mix, stir at 60 °C for 60 min, and use ultrasonic-assisted extraction during this period, with the ultrasonic power being 70 W, the ultrasonic time being 60 min, and the ultrasonic temperature being 60 °C; the deep eutectic solvent used is composed of betaine, propylene glycol and glycerol, and their mass-volume ratio is betaine:propylene glycol:glycerol = 11 g:2 mL:4 mL. Then centrifuge and filter, and collect the filtrate to obtain Patrinia villosa Juss. extract-3.

[0086] The preparation method of Patrinia villosa Juss. extract-4 is as follows:

[0087] Take commercially available Cynanchum stauntonii (Decne.) Schltr. ex Levl., crush it and sieve it through a 40-mesh sieve, add it to 25 times the amount of a 15% deep eutectic solvent aqueous solution and mix. Stir at 50 °C for 50 min, and use ultrasonic-assisted extraction during this period. The ultrasonic power is 60 W, the ultrasonic time is 50 min, and the ultrasonic temperature is 50 °C. The deep eutectic solvent used is composed of butanediol and malic acid, and their molar ratio is: butanediol: malic acid = 1:4. Then carry out centrifugation and filtration, collect the filtrate, and thus obtain Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-4.

[0088] The preparation method of Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-5 is as follows:

[0089] Take commercially available Cynanchum stauntonii (Decne.) Schltr. ex Levl., crush it and sieve it through a 40-mesh sieve, add it to 25 times the amount of a 15% deep eutectic solvent aqueous solution and mix. Stir at 50 °C for 50 min, and use ultrasonic-assisted extraction during this period. The ultrasonic power is 60 W, the ultrasonic time is 50 min, and the ultrasonic temperature is 50 °C. The deep eutectic solvent used is composed of betaine, citric acid and propylene glycol, and their mass-volume ratio is betaine: citric acid: propylene glycol = 1 g: 2 mL: 2 mL. Then carry out centrifugation and filtration, collect the filtrate, and thus obtain Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-5.

[0090] The preparation method of Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-6 is as follows:

[0091] Take commercially available Cynanchum stauntonii (Decne.) Schltr. ex Levl., clean it and put it in a 50 °C constant temperature oven to dry to constant weight. Grind and crush it and sieve it through a 40-mesh sieve. Take 1 g and add an aqueous solution with 75% mass percentage of ethanol, and the solid-liquid ratio is 1 g: 30 mL; use ultrasonic extraction method, the ultrasonic temperature is 50 °C, the power is 60 W, and the extraction time is 30 min. Collect the product and centrifuge it, take the supernatant, and thus obtain Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-6.

[0092] Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-7 is purchased from: Shaanxi Lvshengyuan Biological Products Manufacturing Co., Ltd., product name:

[0093] Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract solution.

[0094] The preparation method of the whitening and repair composition of the present invention is: mix each component of the whitening and repair composition below 30 °C, stir until uniform, and store at 30 °C.

[0095] Examples and comparative examples

[0096] The whitening and repair composition of Example 1 contains the following components in parts by weight:

[0097] Thermosensitive resveratrol oxide hydrogel-1 1 part, Peucedanum praeruptorum Dunn extract-1 6 parts, Cynanchum stauntonii (Decne.) Schltr. ex Levl. extract-1 1 part, Tephrosia purpurea (L.) Pers. seed extract 0.5 part.

[0098] The whitening and repair composition of Example 2 contains the following components in parts by weight:

[0099] 15 parts of thermosensitive oxidized resveratrol hydrogel, 14 parts of Peucedanum praeruptorum Dunn extract, 13 parts of Premna serratifolia L. extract, 0.1 part of Tephrosia purpurea (L.) Pers. seed extract.

[0100] The whitening and repair composition of Example 3 comprises the following components in parts by weight:

[0101] 13 parts of thermosensitive oxidized resveratrol hydrogel, 15 parts of Peucedanum praeruptorum Dunn extract, 12 parts of Premna serratifolia L. extract, 0.3 part of Tephrosia purpurea (L.) Pers. seed extract.

[0102] The whitening and repair composition of Example 4 comprises the following components in parts by weight:

[0103] 13 parts of thermosensitive oxidized resveratrol hydrogel, 16 parts of Peucedanum praeruptorum Dunn extract, 12 parts of Premna serratifolia L. extract, 0.3 part of Tephrosia purpurea (L.) Pers. seed extract.

[0104] The only difference between the whitening and repair composition of Example 5 and that of Example 1 is that the thermosensitive oxidized resveratrol hydrogel is thermosensitive oxidized resveratrol hydrogel-2.

[0105] The only difference between the whitening and repair composition of Example 6 and that of Example 1 is that the thermosensitive oxidized resveratrol hydrogel is thermosensitive oxidized resveratrol hydrogel-3.

[0106] The only difference between the whitening and repair composition of Example 7 and that of Example 1 is that the thermosensitive oxidized resveratrol hydrogel is thermosensitive oxidized resveratrol hydrogel-4.

[0107] The only difference between the whitening and repair composition of Example 8 and that of Example 1 is that the thermosensitive oxidized resveratrol hydrogel is thermosensitive oxidized resveratrol hydrogel-5.

[0108] The only difference between the whitening and repair composition of Example 9 and that of Example 1 is that the thermosensitive oxidized resveratrol hydrogel is thermosensitive oxidized resveratrol hydrogel-6.

[0109] The only difference between the whitening and repair composition of Example 10 and that of Example 1 is that the Peucedanum praeruptorum Dunn extract is Peucedanum praeruptorum Dunn extract-2.

[0110] The only difference between the whitening and repair composition of Example 11 and that of Example 1 is that the Peucedanum praeruptorum Dunn extract is Peucedanum praeruptorum Dunn extract-3.

[0111] The only difference between the whitening and repair composition of Example 12 and that of Example 1 is that the Peucedanum praeruptorum Dunn extract is Peucedanum praeruptorum Dunn extract-4.

[0112] The only difference between the whitening and repair composition of Example 13 and that of Example 1 is that the Peucedanum praeruptorum Dunn extract is Peucedanum praeruptorum Dunn extract-5.

[0113] The only difference between the whitening and repair composition of Example 14 and that of Example 1 is that the extract of Peucedanum praeruptorum Dunn is the extract of Peucedanum praeruptorum Dunn-2.

[0114] The only difference between the whitening and repair composition of Example 15 and that of Example 1 is that the extract of Peucedanum praeruptorum Dunn is the extract of Peucedanum praeruptorum Dunn-3.

[0115] The only difference between the whitening and repair composition of Example 16 and that of Example 1 is that the extract of Peucedanum praeruptorum Dunn is the extract of Peucedanum praeruptorum Dunn-4.

[0116] The only difference between the whitening and repair composition of Example 17 and that of Example 1 is that the extract of Peucedanum praeruptorum Dunn is the extract of Peucedanum praeruptorum Dunn-5.

[0117] The only difference between the whitening and repair composition of Example 18 and that of Example 1 is that the extract of Peucedanum praeruptorum Dunn is the extract of Peucedanum praeruptorum Dunn-6.

[0118] The only difference between the whitening and repair composition of Example 19 and that of Example 1 is that the extract of Peucedanum praeruptorum Dunn is the extract of Peucedanum praeruptorum Dunn-7.

[0119] The whitening and repair composition of Comparative Example 1 contains the following components in parts by weight:

[0120] Thermosensitive oxidized resveratrol hydrogel-1 0.5 part, Peucedanum praeruptorum Dunn extract-1 8 parts, Peucedanum praeruptorum Dunn extract-1 0.5 part, Tephrosia purpurea seed extract 0.7 part.

[0121] The whitening and repair composition of Comparative Example 2 contains the following components in parts by weight:

[0122] Thermosensitive oxidized resveratrol hydrogel-1 7 parts, Peucedanum praeruptorum Dunn extract-1 2 parts, Peucedanum praeruptorum Dunn extract-15 parts, Tephrosia purpurea seed extract 0.05 part.

[0123] The only difference between the whitening and repair composition of Comparative Example 3 and that of Example 1 is that the Peucedanum praeruptorum Dunn extract-1 is replaced with Angelica sinensis extract.

[0124] The only difference between the whitening and repair composition of Comparative Example 4 and that of Example 1 is that the Peucedanum praeruptorum Dunn extract-1 is replaced with Cynanchum bungei extract.

[0125] The only difference between the whitening and repair composition of Comparative Example 5 and that of Example 1 is that the Tephrosia purpurea seed extract is replaced with Glycine soja seed extract.

[0126] The only difference between the whitening and repair composition of Comparative Example 6 and that of Example 1 is that the oxidized resveratrol hydrogel-1 is not added.

[0127] The only difference between the whitening and repair composition of Comparative Example 7 and Example 1 is that: Peucedanum praeruptorum Dunn extract-1 is not added.

[0128] The only difference between the whitening and repair composition of Comparative Example 8 and Example 1 is that: Thlaspi arvense L. extract-1 is not added.

[0129] The only difference between the whitening and repair composition of Comparative Example 9 and Example 1 is that: Tephrosia purpurea (L.) Pers. seed extract is not added.

[0130] The only difference between the whitening and repair composition of Comparative Example 10 and Example 1 is that: Resveratrol oxide hydrogel-7 is used to replace Thermosensitive resveratrol oxide hydrogel-1.

[0131] Effect Example 1, Temperature responsiveness and loading capacity test of resveratrol oxide hydrogel

[0132] Investigate the influence of resveratrol oxide hydrogel-1 to 7 on the release temperature of resveratrol oxide, and test the loading capacity of the hydrogel for resveratrol oxide.

[0133] Response temperature test method: Take 5 g of hydrogels prepared by different methods in a small glass bottle and seal it. Place the glass bottle in a 20°C water bath and slowly increase the temperature of the water bath at a rate of 0.5°C / min. During this period, slowly rotate the glass bottle 90° multiple times. When it is observed that the gel no longer flows, use a thermometer to measure the water bath temperature, and repeat the measurement three times and take the average value, which is the response temperature.

[0134] Loading capacity test method: Take 1 g of each hydrogel sample and place it in a 37°C water bath, add 5 mL of butanediol solution, keep the water bath at a constant temperature for 24 h, filter, and test the content X of resveratrol oxide in the filtrate. According to the formula: Loading capacity = X×100 / Resveratrol oxide content shown in Table 1, the loading capacity of resveratrol oxide in each hydrogel can be calculated.

[0135] The content X of resveratrol oxide is determined by HPLC. The test method is as follows: Prepare a standard working solution: Use methanol to prepare solutions of resveratrol oxide standard with concentrations of 0.1 μg / mL, 0.5 μg / mL, 1 μg / mL, 2 μg / mL, 5 μg / mL, and 10 μg / mL; Sample test method: Filter the filtrate through a 0.2 μm filter membrane and prepare it for use on the machine. The test conditions are: Chromatographic column C18 - 150 mm×3 mm×2.7 μm, column temperature 35°C, flow rate 0.8 ml / min, DAD detector, detection wavelength 328 nm, injection volume 5 μL, and the mobile phase is shown in Table 2.

[0136] Data of the mobile phase in Table 2

[0137] Time / min A (0.1% phosphoric acid) / % B (methanol) / % 0 10 90 5 10 90 14 80 20 15 10 90 20 10 90

[0138] Calculation is carried out according to the following formula:

[0139]

[0140] Where X is the content of the component to be measured, %; C is the concentration of the solution of the component to be measured obtained from the standard curve, μg / mL; V is the constant volume of the solution, mL; f is the dilution factor; m is the sample weighing amount, g.

[0141] The test results are shown in Table 3 below:

[0142] Table 3: Test Results of Resveratrol Oxide Hydrogel - 1 to 7

[0143]

[0144]

[0145] As can be seen from the above table, resveratrol oxide hydrogel - 7 is a non - thermosensitive hydrogel and does not have temperature responsiveness. However, when the temperature reaches 36 ± 0.1 °C, it dissolves and releases resveratrol oxide. The thermosensitive resveratrol oxide hydrogels - 1 to 6 have good thermosensitive responsiveness. However, the experimental research of the present invention finds that the response temperature of the thermosensitive resveratrol oxide hydrogel - 6 is 31 ± 0.1 °C, and the response temperature is relatively low, which causes the hydrogel to release prematurely when contacting the skin surface layer (for example: the skin surface), resulting in a decrease in the content of resveratrol oxide that penetrates into the skin basal layer, thereby reducing the whitening effect of the composition of the present invention; the response temperature of the thermosensitive resveratrol oxide hydrogel - 5 is 37.5 ± 0.3 °C, and the response temperature is relatively high, which causes the hydrogel to reach a deeper layer of the skin (for example: the dermis layer) before releasing resveratrol oxide, resulting in too late release time of resveratrol oxide, thereby reducing the content of resveratrol oxide in the epidermal basal layer located in the upper layer of the dermis layer. Therefore, the present invention optimizes the components of the thermosensitive resveratrol oxide hydrogel. When using sodium alginate and polyvinylcaprolactam to prepare the thermosensitive hydrogel, its response temperature is between 33.5 and 36 °C, which is closer to the skin basal layer, enabling the hydrogel loaded with resveratrol oxide to release resveratrol oxide concentrated in the skin basal layer and improving the whitening effect of resveratrol oxide.

[0146] In order to increase the release amount of thermosensitive resveratrol oxide in the basal layer of the skin by the thermosensitive resveratrol oxide hydrogel, the content ratios of resveratrol oxide, sodium alginate, and polyvinylcaprolactam in the thermosensitive resveratrol oxide hydrogel were optimized. It was found that when the mass ratio of resveratrol oxide to polyvinylcaprolactam in the hydrogel was 1:(9 - 11), and the mass ratio of sodium alginate to polyvinylcaprolactam was 1:(22 - 28), the release temperature of the hydrogel was closer to the optimal temperature of 34 - 35 °C in the basal layer of the skin. Especially when the mass ratio of resveratrol oxide to polyvinylcaprolactam was 1:11, and the mass ratio of sodium alginate to polyvinylcaprolactam was 1:27.5 (thermosensitive resveratrol oxide hydrogel - 1), the release effect and thermosensitivity of the hydrogel were optimal, and its response temperature was closer to the basal layer of the skin, ensuring that resveratrol oxide was released when the thermosensitive hydrogel reached the basal layer of the skin, without being released too early or too late, resulting in the highest content in the basal layer of the skin and ensuring the best whitening effect of the composition.

[0147] Effect Example 2: Test on the Tyrosinase Inhibitory Ability of the Whitening and Repairing Composition

[0148] Test method:

[0149] Treatment of the test substance: Take equal amounts of the compositions prepared in the examples and comparative examples as test samples, and dilute them appropriately with PBS solution;

[0150] Prepare test tubes A, B, C, and D for each test sample according to the following preparation requirements, prepare a blank control tube, and after preparation, add 0.5 mL of L - dopa solution (where the mass percentage content of L - dopa is 5%) to each tube. Keep the blank control in a constant temperature reaction at 37 °C for 30 min (each hydrogel has dissolved), and measure the absorbance value at 475 nm respectively. Each sample is repeated 3 times;

[0151] The solution preparation requirements for test tubes A, B, C, and D are as follows: Tube A: 3 mL of PBS buffer and 0.5 mL of tyrosinase; Tube B: 3.5 mL of PBS buffer; Tube C: 1 mL of sample solution, 2 mL of PBS buffer, and 0.5 mL of tyrosinase; Tube D: 1 mL of sample solution and 2.5 mL of PBS buffer;

[0152] Zero adjustment: PBS solution;

[0153] Calculate the tyrosinase inhibition rate of the test sample according to the formula. The calculation formula for the tyrosinase inhibition rate is:

[0154] Tyrosinase inhibition rate % = 1 - (C - D) / (A - B) × 100%; where A is the absorbance value of tube A, B is the absorbance value of tube B, C is the absorbance value of tube C, and D is the absorbance value of tube D.

[0155] The test results are shown in Table 4 below:

[0156] Table 4: Test results of the tyrosinase inhibitory ability of the whitening and repair composition prepared in the examples and comparative examples

[0157]

[0158]

[0159] As can be seen from the above table, the whitening and repair composition provided by the present invention can significantly increase the tyrosinase inhibition rate, indicating that the combination of the present invention has a strong whitening effect.

[0160] It can be seen from Examples 1-4 and Comparative Examples 1-2 that the weight parts of the components in the whitening and repair composition will affect the whitening effect of the composition. When the weight parts of the composition are within the scope defined by the present invention, it has a good whitening effect. Especially when the weight parts of each component of the composition are the ratio of Example 3, the whitening effect of the composition is better than that of Examples 1 and 2; it can be seen from Example 4 that the mass ratio of the extract of Peucedanum praeruptorum Dunn to the extract of Peucedanum lanceolatum Tausch has a great influence on the whitening effect of the composition. When the mass ratio of the extract of Peucedanum praeruptorum Dunn to the extract of Peucedanum lanceolatum Tausch is 3:1, the whitening effect of the composition is the best.

[0161] The present invention studied the influence of thermosensitive oxidized resveratrol hydrogels obtained by different preparation methods on the ability of the composition to inhibit tyrosinase through Examples 5-9. It can be seen from Examples 5-9 that the preparation method of the thermosensitive oxidized resveratrol hydrogel has a certain influence on the effect of inhibiting tyrosinase, thus affecting the whitening effect of the composition. When using thermosensitive oxidized resveratrol hydrogel-1, the obtained composition has the best whitening effect.

[0162] The present invention studied the influence of the extract of Peucedanum praeruptorum Dunn obtained by different extraction methods on the ability of the composition to inhibit tyrosinase through Examples 10-13. It can be seen from Examples 10-13 that the extraction method of the extract of Peucedanum praeruptorum Dunn has a certain influence on the effect of inhibiting tyrosinase, thus affecting the whitening effect of the composition. When using the extract of Peucedanum praeruptorum Dunn-1, the obtained composition has a better whitening effect.

[0163] The present invention studied the influence of the extract of Peucedanum lanceolatum Tausch obtained by different extraction methods on the ability of the composition to inhibit tyrosinase through Examples 14-19. It can be seen from Examples 14-19 that the extraction method of the extract of Peucedanum lanceolatum Tausch has a certain influence on the effect of inhibiting tyrosinase, thus affecting the whitening effect of the composition. When using the extract of Peucedanum lanceolatum Tausch-1, the obtained composition has a better whitening effect.

[0164] The tyrosinase inhibition rates of Comparative Examples 1-2 were lower than those of the Examples. This was because the mass ratios of the components in the compositions of Comparative Examples 1-2 were not within the scope of the present invention, which affected the synergistic effect between the components and reduced the efficacy of the composition.

[0165] The tyrosinase inhibition rates of Comparative Examples 3-5 were lower than those of the Examples. This was because in the compositions of Comparative Examples 3-5, one of the components in the combination group of the present invention was replaced with other plant extracts, which did not produce a synergistic effect with the specific components selected by the present invention and also affected the synergistic effect between other components of the present invention, reducing the efficacy of the composition.

[0166] The tyrosinase inhibition rates of Comparative Examples 6-9 were lower than those of the Examples. This was because the lack of one of the components in the compositions of Comparative Examples 6-9 affected the synergistic effect between the components and reduced the efficacy of the composition.

[0167] The tyrosinase inhibition rate of Comparative Example 10 was 70.31%. Although resveratrol oxide was released, it did not have skin permeability and could not penetrate resveratrol oxide into the skin, not meeting the requirements of the present invention.

[0168] Application Examples

[0169] The application examples and comparative application examples of the present invention provide a whitening product. The components (mass percentages) of the whitening product are shown in Table 5. Among them, the whitening compositions used in Application Examples 1-19 are the whitening and repairing compositions prepared in Examples 1-19 respectively; the whitening and repairing compositions used in Comparative Application Examples 1-10 are the whitening and repairing compositions prepared in Comparative Examples 1-10 respectively. In addition, a blank application example is set up. The only difference between the blank application example and Application Example 1 is that the whitening and repairing composition is not added.

[0170] Table 5: Components and their mass percentages of the whitening products in the application examples and comparative application examples

[0171]

[0172] The preparation method of the whitening product is as follows:

[0173] Mix the emollient, emulsifier, and antioxidant and heat to 80 °C to obtain the oil phase; mix the thickener, stabilizer, and water and heat to 80 °C to obtain the water phase; add the oil phase to the water phase, homogenize to obtain an emulsion; after cooling, add the active ingredient and preservative to obtain the whitening product.

[0174] Test Example 1. Testing the whitening, repairing, and skin elasticity effects of the whitening product

[0175] Test method: Asian subjects aged 18 - 45 were screened to participate in the test. After the subjects cleaned their faces with a facial cleanser and blotted dry with a lint - free absorbent tissue, they sat still for at least 30 minutes until the skin condition was stable. Then, a color probe Colorimeter CL400, a skin trans - epidermal water loss probe TM Hex, and a skin elasticity tester MPA580 were used to measure the initial value of skin ITA°, the initial value of TEWL, and the initial skin elasticity respectively. The subjects were randomly divided into 14 groups, with 5 people in each group. The whitening products obtained from the application examples and comparative application examples were randomly applied to the whole face. Tests were conducted on the 14th day and 28th day, and the improvement of each volunteer on the 14th day and 28th day was analyzed. The improvement was represented by the improvement rate, and the calculation method was as follows:

[0176] Improvement rate relative to the initial value = |(X 使用后 - X 使用前 ) / X 使用前 ×100%|. Among them, the larger the improvement rate% relative to the initial value, the stronger the efficacy. The results are shown in Table 6 below:

[0177] Table 6: Test results of the whitening products in the application examples and comparative application examples

[0178]

[0179] As can be seen from the above table, the whitening products provided by the present invention can significantly improve the improvement rate of ITA° value, the improvement rate of TEWL, and the improvement rate of elasticity, indicating that the whitening products have strong comprehensive effects of whitening, repair, and improving skin elasticity.

[0180] From Application Examples 1 and 4, it can be seen that the weight parts of the components in the whitening and repair composition will affect the whitening, repair, and skin elasticity improvement effects of the whitening products. From Application Example 4, it can be known that the mass ratio of the extract of Peucedanum praeruptorum Dunn to the extract of Premna serratifolia Linn has a great influence on the whitening, repair, and skin elasticity improvement effects of the composition. When the mass ratio of the extract of Peucedanum praeruptorum Dunn to the extract of Premna serratifolia Linn is 3:1, the effects of whitening, repair, and skin elasticity improvement of the whitening product are the best.

[0181] The present invention studied the influence of thermosensitive resveratrol oxide hydrogels obtained by different extraction methods on the ability of whitening products to improve the improvement rate of ITA° value, the improvement rate of TEWL, and the improvement rate of elasticity through Application Examples 1, 5, and 6. From Application Examples 5 and 6, it can be seen that the preparation method of the thermosensitive resveratrol oxide hydrogel has a certain influence on the ability to improve the improvement rate of ITA° value, the improvement rate of TEWL, and the improvement rate of elasticity, thus affecting the whitening, repair, and skin elasticity improvement effects of the whitening products. When using resveratrol oxide hydrogel - 1, the effects of whitening, repair, and skin elasticity improvement of the obtained whitening product are the best.

[0182] In this invention, Application Examples 1, 10, and 11 were used to study the effects of Peucedanum praeruptorum Dunn extracts obtained by different extraction methods on the abilities of whitening products to improve the improvement rate of ITA°, the improvement rate of TEWL, and the improvement rate of elasticity. It can be seen from Application Examples 10 and 11 that the extraction method of Peucedanum praeruptorum Dunn extract has a certain impact on the abilities to improve the improvement rate of ITA°, the improvement rate of TEWL, and the improvement rate of elasticity, thus affecting the whitening, repair, and skin elasticity improvement effects of whitening products. When using Peucedanum praeruptorum Dunn extract - 1, the whitening, repair, and skin elasticity improvement effects of the obtained whitening products are better.

[0183] In this invention, Application Examples 1, 14, and 15 were used to study the effects of Patrinia villosa Juss. extracts obtained by different extraction methods on the abilities of whitening products to improve the improvement rate of ITA°, the improvement rate of TEWL, and the improvement rate of elasticity. It can be seen from Application Examples 14 and 15 that the extraction method of Patrinia villosa Juss. extract has a certain impact on the abilities to improve the improvement rate of ITA°, the improvement rate of TEWL, and the improvement rate of elasticity, thus affecting the whitening, repair, and skin elasticity improvement effects of whitening products. When using Patrinia villosa Juss. extract - 1, the whitening, repair, and skin elasticity improvement effects of the obtained whitening products are better.

[0184] Comparing Application Examples 3 and 4, the improvement rate of ITA° after 28 days is 22.3 - 22.9%, the improvement rate of TEWL is 14.2 - 15.2%, and the improvement rate of elasticity is 24.6 - 24.7%. This is because in Application Examples 3 and 4, one of the plant extracts in the whitening repair combination group of this invention was replaced in the whitening products, which did not produce a synergistic effect with the specific components selected in this invention and also affected the synergistic effect among other components of this invention, reducing the efficacy of the composition.

[0185] Comparing Application Examples 6 and 7, the improvement rate of ITA° after 28 days is 19.5 - 19.9%, the improvement rate of TEWL is 11.4 - 12.2%, and the improvement rate of elasticity is 21.5 - 22.7%. This is because in Application Examples 6 and 7, one of the components of the whitening repair composition in this invention was missing in the whitening products, which affected the synergistic effect among the components and reduced the efficacy of the whitening products.

[0186] For Comparative Application Example 10, the improvement rate of ITA° after 28 days is 21.6%, the improvement rate of TEWL is 12.7%, and the improvement rate of elasticity is 21.4%. This is because the hydrogel in the whitening product of Comparative Example 10 uses a non - temperature - sensitive hydrogel, which is difficult to release resveratrol oxide through the skin surface temperature, thus reducing the efficacy of the whitening product.

[0187] Test Example 2: Use test of the whitening product on consumers with sensitive skin

[0188] Questionnaire: 30 Asian subjects with sensitive facial skin (positive lactic acid stinging test) and recent symptoms of sensitive skin were selected to participate in the test. The skin care products of Application Example 4 were distributed to them, and each subject applied an appropriate amount of the product on the face according to 2 mg / cm 2 for a 28-day use test. Consumers completed a questionnaire on the improvement degree of indicators such as usage feeling, discomfort, tolerance, mildness and non-irritation. The test results are shown in the following table:

[0189] Table 7: Test Results of Sensitive Skin Consumers Using Whitening Products

[0190]

[0191]

[0192] As can be seen from the above table, the whitening product of the present invention added with the whitening and repair composition has the effects of whitening, repair, improving skin elasticity, etc.

[0193] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A whitening and repairing composition, characterized in that: The composition comprises the following components in parts by weight: 1-5 parts of thermosensitive oxidized resveratrol hydrogel, 4-6 parts of Peucedanum pekinensis extract, 1-3 parts of Salix babylonica extract, and 0.1-0.5 parts of Sophora flavescens seed extract.

2. The whitening and repairing composition according to claim 1, characterized in that: The mass ratio of the Peucedanum peucedanum extract to the Willow Leaf Glehnia extract is: Peucedanum peucedanum extract: Willow Leaf Glehnia extract=(2.5-3):

1.

3. The whitening and repairing composition according to claim 1, characterized in that: The temperature-sensitive oxidized resveratrol hydrogel comprises the following components in percentage by mass: 1-3% oxidized resveratrol, 0.5-1% sodium alginate, 15-25% polyvinyl caprolactam, 0.4-0.6% ammonium persulfate, 20-30% butanediol, and the balance water.

4. The whitening and repairing composition according to claim 3, characterized in that: The mass ratio of the oxidized resveratrol to polyvinyl caprolactam is: oxidized resveratrol: polyvinyl caprolactam = 1: (9-11).

5. The whitening and repairing composition according to claim 3, characterized in that: The mass ratio of the sodium alginate to polyvinyl caprolactam is: sodium alginate:polyvinyl caprolactam=1:(22-28).

6. The whitening and repairing composition according to any one of claims 1 to 5, characterized in that: The preparation method of the Peucedanum peucedanum extract comprises the following steps: The Peucedanum peucedanum is dried and crushed, added into an ethanol solution, ultrasonically extracted, filtered, and the filtrate is concentrated to obtain the Peucedanum peucedanum extract.

7. The whitening and repairing composition according to claim 6, characterized in that: Satisfy at least one of the following: (a) the ethanol concentration in the ethanol solution is 60-70%; (b) the power of the ultrasonic extraction is 100-120W; (c) The ultrasonic extraction time is 30-60 min.

8. The whitening and repairing composition according to any one of claims 1 to 5, characterized in that: The preparation method of the willow leaf leucoderma extract comprises the following steps: The extract of Psoralea corylifolia is obtained by drying and crushing the Psoralea corylifolia and adding the Psoralea corylifolia to an aqueous solution of a deep eutectic solvent for ultrasonic extraction, followed by centrifugation and filtration, and collecting the filtrate to obtain the Psoralea corylifolia extract; wherein the deep eutectic solvent is composed of betaine, propylene glycol and glycerol.

9. The whitening and repairing composition according to claim 8, characterized in that: Satisfy at least one of the following: (a) the mass volume ratio of betaine, propylene glycol and glycerol is: betaine: propylene glycol: glycerol = 9-11 g: 2-4 mL: 2-4 mL; (b) the mass concentration of the deep eutectic solvent in the aqueous solution of the deep eutectic solvent is 10-20%; (d) the power of the ultrasonic extraction is 60-70W; (e) The ultrasonic extraction time is 30-60 min.

10. A whitening product, characterized in that: The whitening product comprises the whitening and repairing composition according to any one of claims 1 to 9.

Images