Wound flushing fluid with good sterilization effect and preparation method thereof

Abstract

The invention discloses a wound flushing fluid with a good sterilization effect and a preparation method thereof, and belongs to the field of medical treatment. The wound cleaning fluid takes HCIO as a main material and comprises the following components in percentage by mass: 50 to 60 percent of HCIO, 20 to 30 percent of HCI, 5 to 15 percent of ethanol, 0.005 to 0.01 percent of trace elements, 0.2 to 1.3 percent of an active agent and 0.1 to 0.5 percent of a chelating agent. The wound cleaning fluid is prepared from the following components in percentage by mass: 50 to 60 percent of HCIO, 20 to 30 percent of HCI, 5 to 15 percent of ethanol, 0.005 to 0.01 percent of trace elements, 0.2 to 1.3 percent of an active agent and 0.1 to 0.5 percent of a chelating agent. According to the invention, the anionic surfactant lauryl sodium sulfate and the cationic surfactant benzalkonium bromide are added into the wound flushing fluid taking HCIO as a main component, so that the surface tension of the fluid can be reduced, and the wettability and permeability of HICO potential water are enhanced, so that the HICO potential water is easier to contact and permeate into the surface of an object or the deep part of a wound, and the cleaning and sterilizing effects are improved; the wound flushing fluid has the advantages that the permeability of the wound flushing fluid is improved, the sterilizing effect is improved, and the wound flushing fluid has certain emulsifying and dispersing effects and can help to remove organic pollutants such as oil stains and bloodstains.

Description

Technical Field

[0001] The invention relates to the technical field of wound cleaning, and in particular to a wound washing liquid with good sterilization effect and a preparation method thereof. Background Art

[0002] Wound irrigation is a crucial part of wound management. Its purpose is to hydrate the wound, remove deep debris, and assist medical staff in a more comprehensive examination of the wound. The irrigation solution can effectively remove cellular debris, surface microorganisms, residual local drugs and dressings. In surgical operations, sterile saline is a commonly used wound irrigation solution. It can keep the surgical field clear, facilitate the inspection of active bleeding, and at the same time flush residual blood and tissue, maintain the cleanliness of the surgical incision, and reduce the risk of incision infection. However, existing wound irrigation solutions have certain limitations. Although saline and Ringer's solution are widely used, they have no obvious hemostatic properties. , and it is difficult to promote wound healing. Hydrogen peroxide is toxic to fibroblasts, and there is a risk of dissolving blood clots, causing bleeding, forming subcutaneous emphysema and even gas embolism, and it will damage the foam dressing structure. Its clinical use is subject to many restrictions. From the existing technology, common wound cleaning fluids use normal saline as the main material, which has poor bactericidal effect. The wound is easily invaded by bacteria during the washing process and has poor antibacterial effect. The rare addition of various additives to normal saline can only slightly improve the bactericidal ability, but does not have the effect of promoting wound healing. To this end, the present invention provides a wound washing fluid with good sterilization effect and a preparation method thereof. Summary of the invention

[0003] The purpose of the present invention is to provide a wound washing liquid with good sterilization effect and a preparation method thereof in view of the above technical problems. The wound washing liquid is mainly composed of HCIO, including HCIO, HCl, ethanol, trace elements, active agents, and chelating agents, wherein the mass fraction of HCIO is 50%-60%, the mass fraction of HCl is 20%-30%, the mass fraction of ethanol is 5%-15%, the mass fraction of trace elements is 0.005%-0.01%, the mass fraction of active agents is 0.2%-1.3%, and the mass fraction of chelating agents is 0.1%-0.5%.

[0004] Preferably, the active agent is divided into a cationic surfactant and an anionic surfactant, the cationic surfactant is benzalkonium bromide, and the anionic surfactant is sodium dodecyl sulfate.

[0005] Preferably, the chelating agent is EDTA.

[0006] Preferably, the trace element is zinc ion, which is added into the wound washing solution using zinc salt solution as a carrier.

[0007] A method for preparing a wound flushing solution with good sterilization effect comprises the following preparation steps:

[0008] S1: dissolving an appropriate amount of sodium chloride in deionized water to prepare a sodium chloride solution of a certain concentration;

[0009] S2: The prepared sodium chloride solution is electrolyzed. Under the action of the electric field, an oxidation reaction occurs at the anode, and the chloride ions are oxidized to generate chlorine gas, which dissolves in water to generate strong oxidizing substances such as hypochlorous acid; a reduction reaction occurs at the cathode, and water is reduced to generate hydrogen gas and hydroxide ions;

[0010] S3: preparing a zinc salt solution, weighing zinc sulfate crystals, placing the zinc sulfate crystals in a beaker, adding an appropriate amount of deionized water, and stirring with a glass rod to completely dissolve the zinc sulfate crystals to obtain a zinc salt solution;

[0011] S4: Prepare a container, first add an appropriate amount of prepared HICO electric water according to the calculated amount, then slowly add sodium dodecyl sulfate, benzalkonium bromide, ethanol, zinc salt solution, and EDTA in sequence. Stir continuously during the addition process;

[0012] S5: After mixing evenly, test various indicators of the solution, including pH value, effective chlorine content and concentration of each additive.

[0013] Preferably, in the above step S1, the concentration of the prepared sodium chloride solution is controlled at 0.1%-0.5%.

[0014] Preferably, in the above step S2, during the electrolysis process, the voltage is 5-15V, the current is 0.5-2A, and the electrolysis time is 10-30 minutes.

[0015] Preferably, in the above step S3, the temperature is controlled at 40-60° C. during the preparation of the zinc salt solution.

[0016] Preferably, in the above step S3, the pH value of the HICO potential water obtained by electrolysis reaches 2.0-3.0, the oxidation-reduction potential (ORP) is ≥1100mV, and the effective chlorine content is 30-150mg / L.

[0017] Preferably, in the above step S4, the stirring speed is controlled at 100-300 rpm.

[0018] The present invention has at least the following beneficial effects:

[0019] In the present invention, the wound washing liquid mainly comprises HClO, which is an acidic aqueous solution with a high redox potential and a strong oxidizing ability, and can destroy bacterial cell membranes, proteins, nucleic acids and other biological macromolecules, thereby achieving a rapid sterilization effect, and at the same time, it also has a good killing effect on viruses, fungi, etc., and has little irritation to human tissues, and can promote wound healing;

[0020] In the present invention, anionic surfactant sodium dodecyl sulfate and cationic surfactant benzalkonium bromide are added to the wound washing liquid mainly composed of HCIO, which can reduce the surface tension of the liquid, enhance the wettability and permeability of HICO potential water, make it easier to contact and penetrate into the surface of the object or the deep of the wound, improve the cleaning and sterilization effect, increase the permeability of the wound washing liquid, improve the sterilization effect, and have a certain emulsification and dispersing effect, which can help remove organic pollutants such as oil stains and blood stains;

[0021] In the present invention, adding ethanol to the wound washing liquid mainly composed of HCIO can accelerate the drying speed of the washing liquid on the surface of the object, reduce the residue, and as a solvent, can improve the overall sterilization effect;

[0022] In the present invention, the zinc salt solution is added to the wound washing solution mainly composed of HCIO, which has a certain antibacterial effect. When added to the HICO potential water, it can synergize with the effective ingredients therein to enhance the inhibition and killing ability of various microorganisms, and at the same time has a certain promoting effect on wound healing, etc., and is conducive to cell proliferation and repair;

[0023] In the present invention, EDTA is added to the wound washing solution mainly composed of HICO to form a stable complex with metal ions in the water, thereby preventing these metal ions from reacting with the components in the HICO potential water and affecting its stability and bactericidal effect.

[0024] Other advantages, objectives and features of the present invention will be embodied in part through the following description, and in part will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 A comparison chart of the bactericidal effects of Example 1 of a wound washing liquid with good sterilization effect and a preparation method thereof and normal saline;

[0026] Figure 2 A comparison chart of the sterilization effects of Example 2, normal saline and Example 1 in a wound washing solution with good sterilization effect and a preparation method thereof;

[0027] Figure 3 A comparison chart of the sterilization effects of Example 3, normal saline and Examples 1-2 in a wound washing solution with good sterilization effect and a preparation method thereof;

[0028] Figure 4 A comparison chart of the sterilization effects of Example 4, normal saline and Examples 1-3 in a wound washing solution with good sterilization effect and a preparation method thereof;

[0029] Figure 5 The present invention is a flow chart of a wound washing solution with good sterilization effect and a preparation method thereof. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0031] The application principle of the present invention is described in detail below in conjunction with the accompanying drawings.

[0032] Embodiment 1:

[0033] (1) dissolving an appropriate amount of sodium chloride in deionized water to prepare a sodium chloride solution with a concentration of 0.5%;

[0034] (2) electrolyzing the prepared sodium chloride solution. Under the action of the electric field, an oxidation reaction occurs at the anode, and the chloride ions are oxidized to generate chlorine gas. The chlorine gas dissolves in water to generate strong oxidizing substances such as hypochlorous acid. A reduction reaction occurs at the cathode, and water is reduced to generate hydrogen gas and hydroxide ions. During the electrolysis process, the voltage is 5-15V, the current is 0.5-2A, and the electrolysis time is 10-30 minutes. The pH value of the HICO potential water obtained by electrolysis reaches 2.0-3.0, the oxidation-reduction potential (ORP) is ≥1100mV, and the effective chlorine content is 30-150mg / L;

[0035] (3) preparing a zinc salt solution, weighing zinc sulfate crystals, placing the zinc sulfate crystals in a beaker, adding an appropriate amount of deionized water, and stirring with a glass rod to completely dissolve the zinc sulfate crystals to obtain a zinc salt solution;

[0036] (4) Prepare a container, first add an appropriate amount of prepared HICO electric water according to the calculated amount, and then slowly add sodium dodecyl sulfate, benzalkonium bromide, ethanol, zinc salt solution, and EDTA in sequence, stirring continuously during the addition process;

[0037] (5) After mixing evenly, the various indicators of the solution were tested, including pH value, effective chlorine content and concentration of each additive, wherein the mass fraction of HCIO was 60%, the mass fraction of HCl was 30%, the mass fraction of ethanol was 9%, the mass fraction of trace elements was 0.01%, the mass fraction of active agent was 0.89%, and the mass fraction of chelating agent was 0.1%.

[0038] The experimental results show that:

[0039] The wound rinsing solution prepared in Example 1 was compared with normal saline on the wound of the animal model, and it was found that the wound rinsing solution prepared in Example 1 had a strong bactericidal and sterilizing effect. For specific comparison, see Figure 1 , and has a certain ability to promote recovery at the wound site, see Table 1, and the specific preparation process is shown in Figure 5 .

[0040] Table 1 is a comparison of the bacterial counts in Example 1 and normal saline at the same wound site.

[0041]

[0042] Embodiment 2:

[0043] (1) dissolving an appropriate amount of sodium chloride in deionized water to prepare a sodium chloride solution with a concentration of 0.5%;

[0044] (2) electrolyzing the prepared sodium chloride solution. Under the action of the electric field, an oxidation reaction occurs at the anode, and the chloride ions are oxidized to generate chlorine gas. The chlorine gas dissolves in water to generate strong oxidizing substances such as hypochlorous acid. A reduction reaction occurs at the cathode, and water is reduced to generate hydrogen gas and hydroxide ions. During the electrolysis process, the voltage is 5-15V, the current is 0.5-2A, and the electrolysis time is 10-30 minutes. The pH value of the HICO potential water obtained by electrolysis reaches 2.0-3.0, the oxidation-reduction potential (ORP) is ≥1100mV, and the effective chlorine content is 30-150mg / L;

[0045] (3) The mass fraction of HCIO is 70% and the mass fraction of HCl is 30%.

[0046] The experimental results show that:

[0047] The wound irrigation prepared in Example 2 was compared with normal saline and Example 1 on the wound of the animal model. Figure 2 The wound washing solution prepared in Example 2 has a strong bactericidal and sterilizing effect, see Table 2.

[0048] Table 2 is a comparison of the bacterial counts in Example 2, saline and Example 1 at the same wound site.

[0049]

[0050] Embodiment 3:

[0051] (1) dissolving an appropriate amount of sodium chloride in deionized water to prepare a sodium chloride solution with a concentration of 0.5%;

[0052] (2) electrolyzing the prepared sodium chloride solution. Under the action of the electric field, an oxidation reaction occurs at the anode, and the chloride ions are oxidized to generate chlorine gas. The chlorine gas dissolves in water to generate strong oxidizing substances such as hypochlorous acid. A reduction reaction occurs at the cathode, and water is reduced to generate hydrogen gas and hydroxide ions. During the electrolysis process, the voltage is 5-15V, the current is 0.5-2A, and the electrolysis time is 10-30 minutes. The pH value of the HICO potential water obtained by electrolysis reaches 2.0-3.0, the oxidation-reduction potential (ORP) is ≥1100mV, and the effective chlorine content is 30-150mg / L;

[0053] (3) preparing a zinc salt solution, weighing zinc sulfate crystals, placing the zinc sulfate crystals in a beaker, adding an appropriate amount of deionized water, and stirring with a glass rod to completely dissolve the zinc sulfate crystals to obtain a zinc salt solution;

[0054] (4) Prepare a container, first add an appropriate amount of prepared HICO electric water according to the calculated amount, and then slowly add the zinc salt solution in sequence, stirring continuously during the addition process;

[0055] (5) After mixing evenly, the various indicators of the solution were tested, including pH value, effective chlorine content and concentration of various additives, wherein the mass fraction of HCIO was 70%, the mass fraction of HCl was 30%, and the mass fraction of trace elements was 0.01%.

[0056] The experimental results show that:

[0057] The wound irrigating solution prepared in Example 3 was compared with physiological saline and Examples 1 and 2 on the wound of the animal model, and it was found that the wound irrigating solution prepared in Example 1 had a stronger bactericidal and sterilizing effect. For specific comparison, see Figure 3 , and it also has a certain ability to promote recovery at the wound site, see Table 3.

[0058] Table 3 is a comparison of the bacterial counts in Example 3, saline, and Examples 1 and 2 at the same wound site.

[0059]

[0060] Embodiment 4:

[0061] (1) dissolving an appropriate amount of sodium chloride in deionized water to prepare a sodium chloride solution with a concentration of 0.5%;

[0062] (2) electrolyzing the prepared sodium chloride solution. Under the action of the electric field, an oxidation reaction occurs at the anode, and the chloride ions are oxidized to generate chlorine gas. The chlorine gas dissolves in water to generate strong oxidizing substances such as hypochlorous acid. A reduction reaction occurs at the cathode, and water is reduced to generate hydrogen gas and hydroxide ions. During the electrolysis process, the voltage is 5-15V, the current is 0.5-2A, and the electrolysis time is 10-30 minutes. The pH value of the HICO potential water obtained by electrolysis reaches 2.0-3.0, the oxidation-reduction potential (ORP) is ≥1100mV, and the effective chlorine content is 30-150mg / L;

[0063] (3) preparing a zinc salt solution, weighing zinc sulfate crystals, placing the zinc sulfate crystals in a beaker, adding an appropriate amount of deionized water, and stirring with a glass rod to completely dissolve the zinc sulfate crystals to obtain a zinc salt solution;

[0064] (4) Prepare a container, first add an appropriate amount of prepared HICO electric water according to the calculated amount, then slowly add zinc salt solution and EDTA in sequence, stirring continuously during the addition process;

[0065] (5) After mixing evenly, various indicators of the solution were tested, including pH value, effective chlorine content and concentration of various additives, wherein the mass fraction of HCIO was 60%, the mass fraction of HCl was 29%, the mass fraction of trace elements was 0.01%, and the mass fraction of chelating agent was 0.1%.

[0066] The experimental results show that:

[0067] The wound rinsing solution prepared in Example 4 was compared with physiological saline and Examples 1, 2, and 3 on the wound of the animal model, and it was found that the wound rinsing solution prepared in Example 4 had a strong bactericidal and sterilizing effect. For specific comparison, see Figure 4 , and it also has a certain ability to promote recovery at the wound site, see Table 4.

[0068] Table 4 is a comparison of the bacterial counts of Example 4, saline, and Examples 1-3 at the same wound site.

[0069]

[0070] The present invention discloses a wound washing liquid with good sterilization effect and a preparation method thereof. The prepared wound washing liquid with good sterilization effect has a rapid sterilization effect and prolongs the sterilization effect. At the same time, it has a certain promoting effect on wound healing and is helpful for cell proliferation and repair. Compared with embodiments 1-4, embodiment 1 has the best effect, can quickly sterilize, has a strong antibacterial effect, and also has a certain function of promoting wound repair.

[0071] The above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Relevant technicians in the field may make reasonable modifications or equivalent substitutions to the present invention, but any modifications and partial substitutions that do not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

[0072] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

Claims

1. A wound washing solution with good sterilization effect, characterized in that: The wound cleaning solution is mainly composed of HCIO, including HCIO, HCI, ethanol, trace elements, active agents, and chelating agents, wherein the mass fraction of HCIO is 50%-60%, the mass fraction of HCI is 20%-30%, the mass fraction of ethanol is 5%-15%, the mass fraction of trace elements is 0.005%-0.01%, the mass fraction of active agents is 0.2%-1.3%, and the mass fraction of chelating agents is 0.1%-0.5%.

2. A wound washing solution with good sterilization effect according to claim 1, characterized in that: The active agent is divided into a cationic surfactant and an anionic surfactant. The cationic surfactant is benzalkonium bromide, and the anionic surfactant is sodium dodecyl sulfate.

3. A wound washing solution with good sterilization effect according to claim 1, characterized in that: The chelating agent is EDTA.

4. A wound washing solution with good sterilization effect according to claim 1, characterized in that: The trace element is zinc ion, which is added into the wound washing solution with zinc salt solution as a carrier.

5. A method for preparing a wound washing solution with good sterilization effect, characterized in that: The method comprises the following preparation steps: S1: dissolving an appropriate amount of sodium chloride in deionized water to prepare a sodium chloride solution of a certain concentration; S2: The prepared sodium chloride solution is electrolyzed. Under the action of the electric field, an oxidation reaction occurs at the anode, and the chloride ions are oxidized to generate chlorine gas, which dissolves in water to generate strong oxidizing substances such as hypochlorous acid; a reduction reaction occurs at the cathode, and water is reduced to generate hydrogen gas and hydroxide ions; S3: preparing a zinc salt solution, weighing zinc sulfate crystals, placing the zinc sulfate crystals in a beaker, adding an appropriate amount of deionized water, and stirring with a glass rod to completely dissolve the zinc sulfate crystals to obtain a zinc salt solution; S4: Prepare a container, first add an appropriate amount of prepared HICO electric water according to the calculated amount, then slowly add sodium dodecyl sulfate, benzalkonium bromide, ethanol, zinc salt solution, and EDTA in sequence. Stir continuously during the addition process; S5: After mixing evenly, test various indicators of the solution, including pH value, effective chlorine content and concentration of each additive.

6. The method for preparing a wound washing solution with good sterilization effect according to claim 5, characterized in that: In the above step S1, the concentration of the prepared sodium chloride solution is controlled at 0.1%-0.5%.

7. The method for preparing a wound washing solution with good sterilization effect according to claim 5, characterized in that: In the above step S2, during the electrolysis process, the voltage is 5-15V, the current is 0.5-2A, and the electrolysis time is 10-30 minutes.

8. The method for preparing a wound washing solution with good sterilization effect according to claim 5, characterized in that: In the above step S3, the temperature is controlled at 40-60° C. during the preparation of the zinc salt solution.

9. The method for preparing a wound washing solution with good sterilization effect according to claim 5, characterized in that: In the above step S3, the pH value of the HICO potential water obtained by electrolysis reaches 2.0-3.0, the oxidation-reduction potential (ORP) is ≥1100mV, and the effective chlorine content is 30-150mg / L.

10. The method for preparing a wound washing solution with good sterilization effect according to claim 5, characterized in that: In the above step S4, the stirring speed is controlled at 100-300 rpm.

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