A method for microencapsulating ozone-degrading, an ozone-degrading solution, and a device

Abstract

The present application belongs to the field of environmental protection technology, and provides a microencapsulated ozone degradation method, ozone degradation liquid and device. The degradation liquid is compounded and designed, and the instant occurrence function of spraying is used to realize efficient degradation corresponding to three kinds of ozone concentrations. The degradation liquid comprises emulsion film bubble liquid and ozone degradation liquid. The spraying device comprises a tank body, an emulsion film bubble liquid storage tank, a degradation liquid storage tank, a gear adjustment, a nozzle and a high-pressure pipeline. The emulsion film bubble liquid storage tank and the ozone degradation liquid storage tank are connected to the nozzle through the high-pressure pipeline. The ozone degradation method is efficient, economical, targeted, non-toxic and non-destructive. The spraying device has a compact and reasonable structure. Each part is connected in turn according to the processing flow by adopting a modular layout to form a continuous and efficient processing system. The key parts are sealed to prevent liquid splashing. At the same time, sensors and other visual monitoring means are provided to ensure the safe, stable and efficient operation of the sprayer.

Description

Technical Field

[0001] The present invention belongs to the technical field of environmental protection, and in particular relates to a method for degrading ozone by microencapsulation, an ozone decomposition solution and a device. Background Art

[0002] Ozone (O3), an allotrope of oxygen (O2), is widely used in industrial disinfection, water treatment, and other fields due to its strong oxidizing properties, excellent disinfection and sterilization, and preservation and preservation properties. However, ozone in the near-surface atmosphere has become a harmful gas, negatively impacting human health, crop growth, and ecosystem balance. The development of effective ozone removal technologies is crucial for environmental protection and human health.

[0003] Controlling precursor emissions and spraying bio-enzymes to eliminate them have been the primary methods for atmospheric ozone control in recent years. National environmental monitoring collects data on atmospheric ozone concentrations. Upon exceeding the standard, fog cannons deployed by environmental protection departments conduct large-scale road patrols, spraying large quantities of bio-enzyme solutions for localized ozone elimination. However, this method suffers from inaccurate localization of polluted areas and the susceptibility of bio-enzyme deactivation, resulting in significant waste. For indoor ozone removal, the only known methods are adsorption and ultraviolet light in air purifiers, in combination with other pollutants. Currently, no effective, dedicated method has been reported.

[0004] Existing ozone removal technologies have many shortcomings:

[0005] Methods for eliminating ozone include chemical absorption, catalytic decomposition, thermal decomposition, etc. Catalytic technology uses transition metal (such as manganese, copper, etc.) oxides or carbon-based (such as activated carbon, graphene, etc.) to decompose ozone molecules under certain temperature and humidity conditions. For example, MnO2 / Al2O3 catalyst is used to promote the decomposition of O3. The activity of the catalyst is affected by environmental factors such as temperature and humidity, and there is a gradual deactivation during use (Fe 2 +→Fe 3 + conversion rate>80% failure) is a common phenomenon, and the catalyst must be replenished or replaced regularly (0.8kg FeSO4 is consumed for every 1kg O3 processed), which leads to increased costs. The catalyst is non-renewable and generates solid waste disposal costs. The method of using photocatalysts to accelerate ozone decomposition requires specific equipment and light sources, which increases equipment investment and limits application scenarios. In addition, the use of UV photolysis to generate free radicals to decompose ozone has low efficiency and high energy consumption. Chemical absorption technology is a method of converting ozone into harmless substances by promoting a chemical reaction with an absorbent (certain reducing agents, alkali solution, etc.). It is generally aimed at high-concentration industrial environments and often has problems such as high energy consumption, complex operation, and easy corrosion. The treatment efficiency is restricted by many factors such as the type and concentration of the absorbent and reaction conditions, and the efficiency is limited. Other harmful substances may be produced during the process, which increases the treatment process and cost.

[0006] The use of the above-mentioned catalysts, photocatalysts, chemical absorption and other methods is only seen in scenarios with high initial ozone concentrations. The ozone concentrations in the ambient atmosphere and indoor air are low, and their use is limited. Summary of the Invention

[0007] The present invention provides a method for microencapsulated ozone degradation, an ozone decomposition solution, and a device to solve the problems in the prior art. The technical solution adopted by the present invention is:

[0008] A method for microencapsulating and degrading ozone molecules, wherein the ozone decomposition solution is sprayed, and the ozone decomposition solution is microencapsulated by membrane bubbles when the spraying occurs, thereby achieving molecular degradation of ozone within three different concentration ranges.

[0009] An ozone decomposition solution, comprising an emulsified membrane bubble solution and an ozone degradation solution;

[0010] The emulsified membrane bubble liquid comprises 0.8-1.5% of polyglycerol fatty acid ester, 0.3-0.6% of lecithin nano liposome and 0.2-0.4% of hydroxypropyl methylcellulose. The emulsified membrane bubble liquid promotes the droplets to spontaneously form microcapsule structures and delays the rupture time of the microcapsules.

[0011] Furthermore, the ozone degradation solution includes a redox solution and a biological enzyme solution;

[0012] The redox solution includes 2-5% L-ascorbic acid, 0.5-1.2% ammonium ferric citrate, 0.3-0.8% sodium thiosulfate, and 10-15% glycerol;

[0013] The biological enzyme solution comprises recombinant catalase with an enzyme activity of 5000-8000 U / mL, superoxide dismutase with an enzyme activity of 2000-4000 U / mL, laccase with an enzyme activity of 1000-2000 U / mL, and 3-5% trehalose.

[0014] Furthermore, the preparation of the emulsified bubble solution includes:

[0015] Liposome preparation: 400 mg of lecithin and 100 mg of cholesterol were dissolved in chloroform, and after rotary evaporation to form a film, 10 mL of PBS buffer was added and ultrasonicated for 15 min to obtain nanoliposomes;

[0016] Oil phase preparation: Dissolve 1.2 g of PGPR in 60°C soybean oil, add the liposome suspension, and homogenize at low speed for 10 min to form the oil phase;

[0017] Preparation of aqueous phase: Soak HPMC in deionized water for 24 hours, heat and stir until completely dissolved, and add Tween-80 to form a transparent colloidal solution;

[0018] Synthesis of emulsion foam: The oil phase and the water phase were mixed in a volume ratio of 3:7 and homogenized under high pressure to form a W / O / W double emulsion.

[0019] Furthermore, the redox solution was prepared by a gradient dissolution method: first, glycerol and deionized water were premixed at a ratio of 1:9, magnetically stirred, and ammonium ferric citrate, sodium thiosulfate, and L-ascorbic acid were added in sequence; the pH was adjusted to 4.5-5.0, and the solution was stored in the dark under nitrogen protection.

[0020] Furthermore, the preparation process of the biological enzyme solution is as follows: 4.5g of trehalose is dissolved in 90ml of Tris-HCl buffer until completely transparent; 0.5g of SOD freeze-dried powder, 0.625g of catalase, and 13.3g of laccase are added in sequence; the enzyme molecules are immobilized by reverse micelle encapsulation, ultrafiltration is concentrated to 100ml, and stored at 4°C in the dark.

[0021] An ozone microencapsulation degradation spray device includes a sprayer tank body 1, an emulsified film bubble liquid storage tank 2, a degradation liquid storage tank 3, an electric regulating valve 4, a fixing card 5, a gear regulating valve 6, a battery compartment 7, a high-pressure pipe 8, a spray switch 9 and a nozzle 10;

[0022] The emulsion film bubble liquid storage tank 2 and the degradation liquid storage tank 3 are arranged in the sprayer tank body 1 , and the emulsion film bubble liquid storage tank 2 and the degradation liquid storage tank 3 are connected to the nozzle 10 through the high-pressure pipe 8 .

[0023] Furthermore, the degradation liquid storage tank 3 stores ozone decomposition liquid, which includes emulsified bubble liquid and ozone degradation liquid; the emulsified bubble liquid storage tank 2 stores emulsified bubble liquid.

[0024] Furthermore, the degradation liquid storage tank 3 stores redox liquid and biological enzyme solution separately and independently, and controls and delivers them through the electric regulating valve 4 configured with the supporting pipeline, so as to realize the three ratios of ozone degradation liquid, redox liquid and biological enzyme solution to meet the degradation of ozone in the corresponding concentration range.

[0025] Furthermore, the volume ratios of the ozone degradation solution, the redox solution, and the biological enzyme solution are as follows:

[0026] When the ozone concentration range is 0-150ug / m 3 When the emulsified membrane bubble solution: redox solution: biological enzyme ratio is 5:1.5:1;

[0027] When the ozone concentration range is 150-300ug / m 3 When the emulsified membrane bubble solution: redox solution: biological enzyme ratio is 7:2.5:1;

[0028] When the ozone concentration range is 300-500ug / m 3When the emulsified membrane bubble liquid: redox solution: biological enzyme is 10:4:1.

[0029] The present invention has the following beneficial effects:

[0030] The ozone degradation solution of the present invention comprises a redox solution and a biological enzyme solution, which are prepared by spraying the solution in three different proportions. The main functional components of the redox solution are L-ascorbic acid and ammonium ferric citrate, which are highly effective in degrading ozone and are non-toxic. Sodium thiosulfate can quickly reduce residual intermediates, promoting the forward reaction and avoiding secondary contamination. The biological enzyme solution is a recombinant enzyme (catalase / SOD / laccase), which is highly specific for ozone degradation and can decompose byproducts in real time. Trehalose can significantly improve the enzyme's thermal stability.

[0031] The emulsified membrane bubble liquid described in the present invention is composed of polyglycerol fatty acid esters (PGPR) and lecithin nanoliposomes, which can form a nanoscale semipermeable membrane. The membrane bubble forms microcapsules that encapsulate ozone molecules and ozone digestion liquid. Hydroxypropyl methylcellulose has the effect of delaying the rupture of microcapsules, extending the effective ozone degradation time from 2-5 seconds of traditional sprays to 20-30 seconds. Through the three-level synergistic mechanism of "physical encapsulation-chemical degradation-enzymatic degradation", "active targeted elimination" of ozone is achieved, with a synergistic degradation efficiency of 98.7%. The final products are O2 and H2O.

[0032] The present invention separately fills the upper and lower compartments of the degradation liquid storage tank with two specially formulated ozone degradation liquids. Simultaneously, an emulsified membrane bubble liquid is prepared and filled into the emulsified membrane bubble liquid storage tank. Upon opening the spray pressure valve, the two degradation liquids are automatically proportioned to form an ozone decomposition liquid of the corresponding level, which is sprayed simultaneously with the emulsified membrane bubble liquid to form instant membrane bubble microcapsules, capturing and delaying the encapsulation of ozone molecules, fully degrading the corresponding concentration of air ozone. This automatically proportioned "microencapsulated synergistic degradation system" achieves efficient, harmless, targeted degradation of ozone through the three-stage synergy of physical encapsulation, chemical redox, and enzyme catalysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the overall structure of the present invention. DETAILED DESCRIPTION

[0034] The following is a combination of the embodiments of the present invention Figure 1 , the technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.

[0035] like Figure 1, an ozone microencapsulated degradation sprayer, comprising a sprayer tank body 1, an emulsified film bubble liquid storage tank 2, a digestion liquid storage tank 3, an electric regulating valve 4, a fixing card 5, a gear regulating valve 6, a battery compartment 7, a high-pressure pipe 8, a spray pressure switch 9 and a nozzle 10;

[0036] The emulsion film bubble liquid storage tank 2 and the digestion liquid storage tank 3 are arranged in the sprayer tank body 1. The emulsion film bubble liquid storage tank 2 and the digestion liquid storage tank 3 are connected to the nozzle 10 through the high-pressure pipeline 8 via the gear regulating valve 6.

[0037] The structural design of the present invention is compact and reasonable. By adopting a modular layout and connecting various parts in sequence according to the processing flow, a continuous and efficient processing system is formed, ensuring the safe, stable and efficient operation of the sprayer.

[0038] The degradation liquid storage tank 3 stores redox liquid and biological enzyme solution separately and independently, and controls and delivers them through the electric regulating valve 4 configured with the supporting pipeline, so as to realize the three ratios of ozone degradation liquid, redox liquid and biological enzyme solution to meet the degradation of ozone in the corresponding concentration range.

[0039] The present invention is based on the ozone concentration gradient and achieves the optimal balance of ozone capture capacity, reaction rate and resource consumption by dynamically adjusting the ratio of emulsion, redox solution and biological enzyme. Therefore, the basic compounding scheme set is: emulsion bubble liquid: degradation liquid = 2: 1 (volume ratio). The scheme is dominated by emulsion to ensure the sufficient number of microcapsules, and the degradation liquid maintains the minimum effective concentration of oxidant and enzyme (ascorbic acid ≥ 0.5%, enzyme activity ≥ 3000U / mL), which is characterized in that it not only ensures the basic degradation efficiency, but also reduces the waste of reagents in low-concentration ozone scenarios.

[0040] When the ozone concentration range is 0-150ug / m 3 When the ozone concentration is low, the priority is to ensure that the microcapsule coverage is greater than 95%. On the basis of maintaining the degradation rate (≥90%), the amount of oxidant / enzyme is reduced to reduce costs. Therefore, the emulsified membrane bubble liquid: redox solution: biological enzyme (volume ratio) is 5:1.5:1; when the ozone concentration range is 150-300ug / m 3 At medium concentration, the capture efficiency and degradation efficiency should be balanced, the ozone capture capacity should be improved, the microcapsule packaging time should be prolonged, and the proportion of oxidants should be appropriately increased to cope with the increase in ozone concentration and maintain the effectiveness of the enzyme reaction. Therefore, the emulsified membrane bubble liquid: redox solution: biological enzyme (volume ratio) is 7:2.5:1, the ozone retention time is extended to 25 seconds, and the degradation rate is increased to 95%; when the ozone concentration range is 300-500ug / m 3When the concentration is high, the microcapsule generation should be maximized to ensure the encapsulation ability and anti-ozone escape ability of high-concentration ozone, strengthen the oxidant concentration to achieve rapid digestion of high-concentration ozone, and control the ratio of enzymes. Therefore, the emulsified membrane bubble liquid: redox liquid: biological enzyme (volume ratio) is 10:4:1, the peak degradation efficiency is as high as 98.7%, and the anti-ozone escape ability is increased by 3 times, which is suitable for emergency scenarios.

[0041] Therefore, the three volume ratios of the ozone degradation solution, redox solution and enzyme solution of the present invention are respectively:

[0042] When the ozone concentration range is 0-150ug / m 3 When the emulsified membrane bubble solution: redox solution: biological enzyme ratio is 5:1.5:1;

[0043] When the ozone concentration range is 150-300ug / m 3 When the emulsified membrane bubble solution: redox solution: biological enzyme ratio is 7:2.5:1;

[0044] When the ozone concentration range is 300-500ug / m 3 When the emulsified membrane bubble liquid: redox solution: biological enzyme is 10:4:1.

[0045] Furthermore, the high-pressure pipe 8 is connected to the nozzle 10 through the spray pressure switch 9 .

[0046] Furthermore, the emulsion bubble liquid storage tank 2 stores an emulsion bubble liquid, which comprises: 0.8-1.5% of polyglycerol fatty acid ester (PGPR); a non-ionic surfactant, which promotes the spontaneous formation of a microcapsule structure by the droplets by reducing the gas-liquid interfacial tension. A concentration below 0.8% cannot form a continuous film layer, and a concentration above 1.5% causes the emulsion viscosity to be too high, affecting the atomization performance; 0.3-0.6% of lecithin nanoliposomes, which are embedded in the PGPR film layer to form a "mosaic structure" to regulate the diffusion rate of ozone molecules. This concentration can make the liposome coverage rate on the PGPR film layer ≥70%; and 0.2-0.4% of hydroxypropyl methylcellulose (HPMC). This concentration range can form a shear-thinning gel (viscosity 200-500 mPa·s), and the film thickness is linearly related to the concentration (0.1%≈1 μm), delaying the microcapsule rupture time and prolonging the ozone residence.

[0047] Furthermore, the emulsified membrane bubble liquid preparation process includes: 1. liposome preparation: 400 mg of lecithin and 100 mg of cholesterol (4:1) are dissolved in chloroform, and after rotary evaporation to form a film, 10 mL of PBS buffer (pH 7.4) is added, and ultrasonic treatment is performed for 15 minutes to obtain nanoliposomes (DLS detection particle size 105±15 nm); 2. oil phase preparation: 1.2 g of PGPR is dissolved in 60°C soybean oil (carrier phase), a liposome suspension (mass fraction 0.5%) is added, and low speed (5000 rpm) homogenization is performed for 10 minutes to form an oil phase; 3. aqueous phase preparation: HPMC is soaked in deionized water for 24 hours, heated (70°C) and stirred (500 rpm) until completely dissolved, and Tween-80 is added to form a transparent colloidal solution; 4. emulsified membrane bubble liquid synthesis: the oil phase and the aqueous phase are mixed in a volume ratio of 3:7, and a W / O / W double emulsion is formed by high-pressure homogenization (20 MPa, 3 cycles).

[0048] Furthermore, the digestion solution storage tank (3) stores redox solution and biological enzyme solution.

[0049] Furthermore, the mass fraction composition of the substances in the redox solution includes: L-ascorbic acid (vitamin C) 2-5%, the main reducing agent, 5% concentration can make the conversion rate of O3 reach 92% when the droplet residence time is less than or equal to 30s; ammonium ferric citrate 0.5-1.2%, the electron transfer medium, this concentration range Fe 3+ / Fe 2+ The ratio is stable at 3:1, with the highest electron transfer efficiency (≥80%); sodium thiosulfate 0.3-0.8%, auxiliary reducing agent, scavenging free radicals; glycerol (carrier) 10-15%, low-temperature protective agent.

[0050] Furthermore, the redox solution was prepared by a gradient dissolution method: first, glycerol and deionized water were premixed at a ratio of 1:9, magnetic stirring was performed (300 rpm), and ammonium ferric citrate, sodium thiosulfate, and L-ascorbic acid were added in sequence; the pH was adjusted to 4.5-5.0, and the mixture was stored in the dark under nitrogen protection;

[0051] Furthermore, the bio-enzyme solution includes recombinant catalase 5000-8000U / mL, and the enzyme activity ≥5000U / mL can achieve real-time decomposition and decompose H2O2 generated by the reaction of residual ozone and water (catalytic efficiency ≥99%); superoxide dismutase (SOD) 2000-4000U / mL, which can effectively remove O2 - Free radicals (half-life <0.1s); laccase 1000-2000U / mL, to match the degradation requirements of by-products (such as formaldehyde / formic acid); trehalose (enzyme stabilizer) 3-5%, which can significantly improve the thermal stability of the enzyme;

[0052] Furthermore, the preparation process of the biological enzyme solution is as follows: 4.5g of trehalose is dissolved in 90ml of Tris-HCl buffer (pH 7.0) until it is completely transparent; 0.5g of SOD freeze-dried powder (specific activity 4000U / mg), 0.625g of catalase (8000U / mg), and 13.3g of laccase (15U / mg) are added in sequence; the enzyme molecules are immobilized by reverse micelle encapsulation, ultrafiltration is concentrated to 100ml, and stored at 4°C in the dark.

[0053] The ozone microencapsulated degradation sprayer of the present invention is widely applicable to occasions where ozone removal is required, including but not limited to:

[0054] Indoor air purification: In indoor environments such as offices and homes, ozone is generated during the operation of air purifiers, printers, computers, and other equipment. Excessive VOCs after interior decoration react with NOx generated by gas combustion to produce ozone through photochemical reactions. The ozone removal liquid involved in the present invention can be atomized by a sprayer to effectively remove ozone gas molecules from the local air, improving air quality and protecting human health.

[0055] Industrial waste gas treatment: Ozone may be produced as a byproduct or intermediate in industries such as power generation, metallurgy, and petrochemicals. Excessive concentrations can harm equipment and personnel. The ozone-eliminating liquid of this invention can be used to treat these industrial waste gases through an automatic spraying method, reducing ozone emission concentrations and complying with environmental regulations.

[0056] Water treatment: Ozone is used as a disinfectant in certain water treatment applications, such as swimming pools and drinking water treatment. In these applications, where excess ozone needs to be removed, the ozone removal solution of the present invention can be used to degrade ozone using a canned sprayer.

[0057] Medical and health care: In the medical field, ozone is sometimes used for disinfection or treatment of certain diseases. The present invention can be used for post-treatment of ozone disinfection of medical equipment to ensure that no ozone residue is left before use.

[0058] Other occasions where ozone removal is required: such as laboratories, scientific research sites, etc., where ozone may be generated during certain chemical reactions or experiments. The present invention can be used to remove ozone in these occasions to ensure the safety and accuracy of the experimental environment.

[0059] The present invention provides a digestion solution, which is obtained by designing a ratio of an emulsifying component and two degradation components. Through nano-atomization and film-forming encapsulation, ozone molecules in the air are captured and microencapsulated.

[0060] The atomization spraying of the ozone degradation liquid can be realized by a sprayer or a fog truck, and the ultrafine mist droplets (<1 mu m) sprayed can capture ozone molecules due to the high sensitivity of the ozone itself, and rapidly build a dynamic semi-permeable membrane microcapsule by using polyglycerol fatty acid ester (PGPR) and lecithin, and realize the efficient targeted elimination of ozone molecules through the synergistic effect of ascorbic acid / iron ion redox system and catalase / SOD enzyme system, so that the ozone is completely degraded into oxygen and water.

[0061] The technical breakthrough advantage lies in: environmental protection, the final product is only oxygen and water, the emulsifier and enzyme system are biodegradable, and there is no secondary pollution; efficiency, the microcapsule wrapping prolongs the effective contact time of ozone and the degradation liquid, at the same time, a stable microenvironment is provided for the degradation reaction, other interference such as oxygen concentration is avoided, the reverse reaction is reduced, and the reaction rate and degradation efficiency are improved. Economic aspect, the present degradation liquid realizes the wrapping reduction of ozone molecules, so that the whole degradation process is environmentally friendly and harmless, no other harmful substances are generated during the treatment process, the degradation products are harmless oxygen and water, no subsequent treatment is needed. The operation cost is low, the product can be recycled, and at the same time, it has wide temperature range adaptability (5-45 DEG C) and open space applicability, and can be widely applied to industrial, medical and public environmental ozone treatment, and has the characteristics of high efficiency, environmental friendliness and low cost.

[0062] The principle of the present application is:

[0063] Small mist beads with certain pressure and diameter range are formed by high-pressure spraying and other technologies. After the degradation liquid is atomized, PGPR and lecithin self-assemble on the surface of the mist droplets to form a core-shell structure.

[0064] Core layer: redox agent (ascorbic acid / iron ion) and biological enzyme solution.

[0065] Shell layer: PGPR-lecithin composite membrane (pore size <0.5 nm)+HPMC gel layer.

[0066] Ozone penetrates the microcapsule membrane into the core layer and reacts with ascorbic acid / iron ion (O3+Fe 2+ →O2+Fe 3+ ), and degrades 80% of the ozone.

[0067] The remaining ozone generates H2O2 (2O3+H2O→3O2+H2O2), and catalase decomposes it into H2O and O2.

[0068] Reaction kinetics process:

[0069] Stage I: Fe 2+ +O3→Fe 3+ +O2 (redox, rapidly consumes 80% of ozone)

[0070] Phase II: 2O3+H2O→3O2+H2O2→catalase catalysis→2H2O+O2 (completely remove the remaining 20%)

[0071] 6.3. Product release and recovery

[0072] O2 is released into the air through the microcapsule membrane, and H2O and Fe(OH)3 colloid are separated by sedimentation and then recycled.

[0073] Online detection of O3 concentration by UV absorption spectroscopy (attenuation rate of characteristic peak 254nm>98%)

[0074] Verification product: O2 characteristic peak 1555cm -1 , intensity ratio>99%, purified gas O2 purity ≥99.2%, by-product H2O aerosol concentration <50μg / m 3 .

[0075] Compared with traditional methods, this invention provides a new solution for the field of ozone elimination. It has the following advantages:

[0076] 1. Environmentally friendly formula (LD50>5000mg / kg)

[0077] a. Redox system:

[0078] Ascorbic acid / ammonium ferric citrate: non-toxic, biodegradable, the reaction product is Fe 3+ (can be precipitated and recovered);

[0079] Sodium thiosulfate: quickly reduces residual oxidants to avoid secondary pollution.

[0080] b. Biological enzyme system:

[0081] Recombinant enzymes (catalase / SOD / laccase): high catalytic efficiency (>10 6 times / minute), strong specificity;

[0082] Trehalose: significantly improves enzyme thermal stability (activity remains >90% at 50°C).

[0083] c. Emulsification / film-forming system:

[0084] PGPR+lecithin: forms a nano-scale semi-permeable membrane (ozone permeability>95%, O2 blocking rate>80%).

[0085] HPMC: prolongs the life of microcapsules.

[0086] 2. High degradation efficiency. The microcapsule structure extends ozone residence time from 2-5 seconds with traditional sprays to 20-30 seconds. Through a three-stage synergistic mechanism of "physical encapsulation, chemical degradation, and enzymatic degradation," active targeted ozone elimination is achieved, with a synergistic degradation efficiency of 98.7%.

[0087] 3. Low risk of secondary pollution. The final products are O2 and H2O (accounting for >99%). No chemical residue: The enzyme system can degrade its own carrier (such as trehalose), and sodium thiosulfate is decomposed into harmless sulfate.

[0088] 4. Wide range of applications. Low cost, suitable for open spaces with a wide temperature range (5-45°C), and can be widely used in industrial, medical and public environment ozone treatment.

[0089] 5. Strong compatibility. Can be directly integrated with existing spray systems (such as high-pressure fine water mist equipment).

[0090] 6. Closed-loop control module. Ozone sensor real-time feedback adjustment.

[0091] Based on the above, the present invention also relates to a method for capturing and degrading ozone molecules by microencapsulation, wherein the ozone decomposition solution is sprayed and the ozone decomposition solution is microencapsulated by the membrane bubble when the spraying occurs, thereby achieving the following three different concentration ranges (0-150ug / m 3 ; 150-300ug / m 3 ; 300-500ug / m 3 )Molecular destruction of ozone.

[0092] According to the above, the present invention also relates to an ozone decomposition solution, including an emulsified membrane bubble solution and an ozone degradation solution;

[0093] The emulsified membrane bubble liquid comprises 0.8-1.5% of polyglycerol fatty acid ester, 0.3-0.6% of lecithin nano liposome and 0.2-0.4% of hydroxypropyl methylcellulose. The emulsified membrane bubble liquid promotes the droplets to spontaneously form microcapsule structures and delays the rupture time of the microcapsules.

[0094] Furthermore, the ozone degradation solution includes a redox solution and a biological enzyme solution;

[0095] The redox solution includes 2-5% L-ascorbic acid, 0.5-1.2% ammonium ferric citrate, 0.3-0.8% sodium thiosulfate, and 10-15% glycerol;

[0096] The biological enzyme solution comprises recombinant catalase with an enzyme activity of 5000-8000 U / mL, superoxide dismutase with an enzyme activity of 2000-4000 U / mL, laccase with an enzyme activity of 1000-2000 U / mL, and 3-5% trehalose.

[0097] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various deformations, modifications, and substitutions made to the technical solutions of the present invention by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A method for capturing and degrading ozone molecules by microencapsulation, characterized in that: By spraying the ozone decomposition liquid, the ozone decomposition liquid is microencapsulated by the membrane bubble when the spraying occurs, and the molecular decomposition of ozone corresponding to three different concentration ranges is achieved; The ozone digestion solution includes an emulsified membrane bubble solution and an ozone degradation solution; the emulsified membrane bubble solution includes 0.8-1.5% of polyglycerol fatty acid ester, 0.3-0.6% of lecithin nanoliposomes, and 0.2-0.4% of hydroxypropyl methylcellulose; Three different concentration ranges include: Ozone concentration range 0-150ug / m³; Ozone concentration range 150-300 ug / m³; Ozone concentration range is 300-500 ug / m³.

2. An ozone decomposition solution, characterized in that: Including emulsified membrane bubble liquid, ozone degradation liquid; The emulsified membrane bubble liquid includes 0.8-1.5% of polyglycerol fatty acid ester, 0.3-0.6% of lecithin nanoliposome, and 0.2-0.4% of hydroxypropyl methylcellulose. The emulsified membrane bubble liquid promotes the droplets to spontaneously form microcapsule structures and delays the rupture time of the microcapsules.

3. An ozone decomposition solution as claimed in claim 2, characterized in that, Ozone degradation liquid includes redox solution and biological enzyme solution; The redox solution includes 2-5% L-ascorbic acid, 0.5-1.2% ammonium ferric citrate, 0.3-0.8% sodium thiosulfate, and 10-15% glycerol; The biological enzyme solution includes recombinant catalase with an enzyme activity of 5000-8000 U / mL, superoxide dismutase with an enzyme activity of 2000-4000 U / mL, laccase with an enzyme activity of 1000-2000 U / mL, and 3-5% trehalose.

4. An ozone decomposition solution as claimed in claim 2, characterized in that, The preparation of the emulsified film bubble liquid comprises: Liposome preparation: 400 mg of lecithin and 100 mg of cholesterol were dissolved in chloroform, rotary evaporated to form a film, and then 10 mL of PBS buffer was added. The film was sonicated for 15 min to obtain nanoliposomes. Preparation of oil phase: Dissolve 1.2 g of PGPR in 60°C soybean oil, add the liposome suspension, and homogenize at low speed for 10 min to form the oil phase; Preparation of aqueous phase: Soak HPMC in deionized water for 24 hours, heat and stir until completely dissolved, and add Tween-80 to form a transparent colloidal solution; Synthesis of emulsion foam: The oil phase and the water phase were mixed in a volume ratio of 3:7 and homogenized under high pressure to form a W / O / W double emulsion.

5. An ozone decomposition solution as claimed in claim 3, characterized in that, The redox solution was prepared by a gradient dissolution method: glycerol and deionized water were premixed at a ratio of 1:9, magnetically stirred, and ammonium ferric citrate, sodium thiosulfate, and L-ascorbic acid were added in sequence; the pH was adjusted to 4.5-5.0, and the solution was stored in the dark under nitrogen protection.

6. An ozone decomposition solution as claimed in claim 3, characterized in that, The enzyme solution was prepared as follows: 4.5 g of trehalose was dissolved in 90 mL of Tris-HCl buffer until completely transparent; 0.5 g of lyophilized SOD powder, 0.625 g of catalase, and 13.3 g of laccase were added in sequence; the enzyme molecules were immobilized using the reverse micelle encapsulation method, and the solution was concentrated to 100 mL by ultrafiltration and stored at 4°C in the dark.

7. An ozone microencapsulated degradation spray device, characterized in that: It includes a sprayer tank body (1), an emulsion film bubble liquid storage tank (2), a degradation liquid storage tank (3), an electric regulating valve (4), a fixing card (5), a gear regulating valve (6), a battery compartment (7), a high-pressure pipe (8), a spray switch (9) and a nozzle (10); The emulsion film bubble liquid storage tank (2) and the degradation liquid storage tank (3) are arranged in the sprayer tank body (1), and the emulsion film bubble liquid storage tank (2) and the degradation liquid storage tank (3) are connected to the nozzle (10) through the high-pressure pipe (8); The degradation liquid storage tank (3) stores ozone decomposition liquid, which includes emulsified film bubble liquid and ozone degradation liquid; the emulsified film bubble liquid storage tank (2) stores emulsified film bubble liquid; The emulsified membrane vesicle solution includes 0.8-1.5% of polyglycerol fatty acid ester, 0.3-0.6% of lecithin nanoliposome, and 0.2-0.4% of hydroxypropyl methylcellulose.

8. An ozone microencapsulated degradation spray device according to claim 7, characterized in that: The degradation liquid storage tank (3) stores the redox liquid and the biological enzyme solution separately and independently, and the electric regulating valve (4) configured in the supporting pipeline is used to control and transport the ozone degradation liquid, the redox liquid, and the biological enzyme solution, thereby achieving the three ratios of ozone degradation liquid, redox liquid, and biological enzyme solution to meet the degradation of ozone within the corresponding concentration range.

9. An ozone microencapsulated degradation spray device according to claim 8, characterized in that: The three volume ratios of ozone degradation solution, redox solution and enzyme solution are: When the ozone concentration range is 0-150ug / m³, the ratio of emulsified membrane bubble liquid: redox liquid: biological enzyme is 5:1.5:1; When the ozone concentration range is 150-300 ug / m³, the ratio of emulsified membrane bubble liquid: redox solution: biological enzyme is 7:2.5:1; When the ozone concentration range is 300-500 ug / m³, the ratio of emulsified membrane bubble liquid: redox liquid: biological enzyme is 10:4:1.

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