Microfluidic detection device and detection method for pathogenic microorganisms

Abstract

The invention discloses a microfluidic detection device and detection method for pathogenic microorganisms, and relates to the technical field of microfluidic detection devices.The microfluidic detection device comprises a rack, a placement table, a detection body, a microchannel and a plurality of detection chips; the cleaning mechanism comprises a clamping assembly, a soot blowing assembly and a dust collection assembly which are mounted at the top of the rack; the clamping assembly comprises a first vertical plate installed on the top of the rack, a connecting frame installed on the top of the first vertical plate, two clamping air cylinders installed at the two ends of the connecting frame correspondingly and two clamping plates installed on piston rods of the two clamping air cylinders correspondingly. And one side of each clamping plate is fixedly connected with a protective layer. According to the application, dust on the surface of the microfluidic detection sheet can be conveniently cleaned.

Description

Technical Field

[0001] The present application relates to the technical field of microfluidic detection devices, and in particular to a microfluidic detection device and a detection method for pathogenic microorganisms. Background Art

[0002] At present, microfluidics refers to the science and technology involved in systems that use microchannels (with dimensions of tens to hundreds of microns) to process or manipulate tiny samples (with volumes of nanoliters to attoliters). It is an emerging interdisciplinary subject involving chemistry, sample physics, microelectronics, new materials, biology, and biomedical engineering. Because of its miniaturization and integration, microfluidic devices are usually called microfluidic chips, also known as chip laboratories and micro-total analysis systems.

[0003] For related technologies, please refer to the Chinese utility model patent with authorization announcement number CN201804009U, which discloses a microfluidic detection sheet, including a body, a microchannel and multiple detection chips. During detection, the microfluidic detection sheet is placed on a placement table, and then the detection liquid enters the inlet end of the microchannel, passes through each detection chip in turn, and flows out at the outlet end of the microchannel at the other end, thereby completing the detection of pathogenic microorganisms.

[0004] However, it is inconvenient to clean the dust on the surface of the microfluidic detection sheet in the prior art, which may easily cause errors in the detection results. Summary of the invention

[0005] The present application provides a microfluidic detection device for pathogenic microorganisms, which is convenient for cleaning dust on the surface of the microfluidic detection sheet, and adopts the following technical solution: A microfluidic detection device for pathogenic microorganisms comprises a frame, a placement table, a detection body, a microchannel and a plurality of detection chips, wherein a cleaning mechanism for cleaning the detection body is installed on the frame, and the cleaning mechanism comprises a clamping assembly, a soot blowing assembly and a dust suction assembly installed on the top of the frame; the clamping assembly comprises a first vertical plate installed on the top of the frame, a connecting frame installed on the top of the first vertical plate, two clamping cylinders respectively installed at both ends of the connecting frame and two clamping plates respectively installed on the piston rods of the two clamping cylinders; the two clamping plates are used to clamp the detection body, and a protective layer is fixedly connected to one side of each of the clamping plates.

[0006] By adopting the above scheme, the detection body is first placed between the two clamping plates, and then the two clamping cylinders are used to drive the two clamping plates to move toward each other, so that the detection body can be clamped; then the detection body is blown away by the soot blowing assembly, and then the dust blown off by the soot blowing assembly is absorbed by the dust suction assembly, so that the detection body can be cleaned; in summary, the cleaning mechanism that is set up facilitates the cleaning of the detection body.

[0007] Preferably, the sootblowing assembly includes a through hole opened in the first vertical plate, a movable plate slidably connected to the through hole, and a driving cylinder installed on one side of the first vertical plate for driving the movable plate to move; an air blowing pipe is installed on the top of the movable plate, and the air blowing pipe is connected to a plurality of nozzles on one side close to the clamping plate, and the nozzles are arranged inclined toward the detection body, and the other side of the air blowing pipe is connected to a fan.

[0008] By adopting the above scheme, when it is necessary to blow soot on the detection body, the movable plate is first driven by the piston rod of the driving cylinder to move toward the detection body. The movement of the movable plate toward the detection body drives the air blowing pipe toward the detection body, and then the fan is started. At this time, the detection body can be soot-blown through the nozzle; in summary, the soot-blowing assembly is convenient for blowing soot on the detection body.

[0009] Preferably, the dust collection assembly includes a second vertical plate fixedly connected to the top of the frame, a horizontal plate installed on one side of the second vertical plate, a first horizontal axis rotatably connected to an end of the horizontal plate away from the second vertical plate, a first connecting plate fixedly connected to the first horizontal axis, a dust collection hood installed on an end of the first connecting plate away from the first horizontal axis, a negative pressure member installed in the dust collection hood, a first gear sleeved and fixed on the first horizontal axis, and a first rack meshed with the first gear; a driving member for driving the first rack to move is installed on the horizontal plate, and an ash holding member is installed on one side of the second vertical plate; in an initial state, the opening of the dust collection hood is downward, which is convenient for the ash holding member to hold ash, and the driving member can set the opening of the dust collection hood toward the soot blowing assembly, which is convenient for ash suction.

[0010] By adopting the above scheme, when the movable plate moves, it can combine with the driving member to drive the first rack to move, the first rack movement drives the first gear to rotate, the first gear rotation drives the first horizontal axis to rotate, the first horizontal axis rotation drives the first connecting plate to rotate, and the rotation of the first connecting plate can make the dust hood be in a vertical state; then start the negative pressure member, and at this time the dust blown off by the soot blowing assembly can be absorbed; in summary, the dust collection assembly set up is convenient for absorbing dust blown off by the soot blowing assembly.

[0011] Preferably, the negative pressure component includes an installation groove opened in the dust hood and a plurality of negative pressure pumps installed in the installation groove; a negative pressure pipe is installed at the inlet of the negative pressure pump, a dust filter plate is installed in the dust hood, and the end of the negative pressure pipe away from the negative pressure pump passes through a side wall of the installation groove and is arranged toward the dust filter plate; the outlet of the negative pressure pump is connected to an air outlet pipe, and the end of the air outlet pipe away from the negative pressure pump passes through the side wall of the installation groove.

[0012] By adopting the above scheme, the provided negative pressure pump and negative pressure pipe facilitate the dust hood to generate suction; the provided dust blocking filter plate can block dust.

[0013] The cam is an assembly made of a first piece and a second piece, and the cam is adapted to engage the first and second gears of the drive means to move the first gear into engagement with the first gear.

[0014] By adopting the above scheme, the movable plate moves and drives the horizontal plate to move. At this time, the horizontal plate drives the first driving plate to move downward under the action of the first inclined plane, and then the first driving plate drives the second driving plate to move under the action of the second inclined plane. The second driving plate moves and drives the first rack to move. The first rack moves and drives the first gear to rotate. The rotation of the first gear drives the first horizontal axis to rotate. The rotation of the first horizontal axis drives the first connecting plate to rotate. The rotation of the first connecting plate can make the dust hood in a vertical state. In summary, the set driving member facilitates the dust hood to be in a vertical state.

[0015] Preferably, the ash containing member comprises a support plate fixedly connected to the side wall of the second vertical plate and an ash receiving box plugged into the top of the support plate; a plug-in plate is fixedly connected to the bottom of the ash receiving box, a slot is provided at the top of the support plate, and the plug-in plate is plugged into the slot.

[0016] By adopting the above scheme, the dust collecting box is provided, which is convenient for collecting the dust falling from the dust collecting hood.

[0017] Preferably, a dust shielding mechanism for shielding dust from the top of the placement table is installed on the top of the frame, and the dust shielding mechanism includes a fixed frame fixedly connected to the top of the frame, a second horizontal axis rotatably connected to the top of the fixed frame, a second connecting plate fixedly connected to the second horizontal axis, and a baffle fixedly connected to the second connecting plate at an end away from the second horizontal axis; a second gear is fixedly mounted on the second horizontal axis, a second rack is meshed on the second gear, and the dust shielding mechanism also includes a transmission assembly for driving the second rack to move; in an initial state, the baffle is in a vertical state, which is convenient for placing the detection body on the top of the placement table for detection, and the transmission assembly can make the baffle horizontal and on the top of the placement table, which is convenient for shielding dust from the top of the placement table.

[0018] By adopting the above scheme, when the movable plate moves, it can combine with the transmission assembly to drive the second rack to move, the second rack movement drives the second gear to rotate, the second gear rotation drives the second horizontal axis to rotate, and the second horizontal axis rotation drives the second connecting plate to rotate, so that the baffle plate can be placed above the placement table, thereby facilitating the reduction of dust falling to the top of the placement table; in summary, the dust blocking mechanism set up can reduce dust falling to the top of the placement table.

[0019] Preferably, the transmission assembly includes a first connecting rod hinged at one end of the moving plate and a third sliding sleeve hinged to an end of the first connecting rod away from the moving plate; a third guide rod is fixedly connected to one side of the first vertical plate, and the third sliding sleeve is slidably connected to the third guide rod; a second connecting rod is hinged to the bottom of the third sliding sleeve, and a pull plate is hinged to the end of the second connecting rod away from the third sliding sleeve, and the end of the pull plate away from the second connecting rod is fixed to one end of the second rack; a fourth sliding sleeve is fixedly connected to the bottom of the pull plate, a fourth guide rod is fixedly connected to the top of the fixed frame, the fourth sliding sleeve is slidably connected to the fourth guide rod, a third stopper is fixedly connected to one end of the fourth guide rod, and a third return spring is arranged between the third stopper and the fourth sliding sleeve.

[0020] By adopting the above scheme, the movement of the movable plate drives the first connecting rod to move, the movement of the first connecting rod drives the movement of the third sliding sleeve, the movement of the third sliding sleeve drives the movement of the second connecting rod, the movement of the second connecting rod drives the pull plate to move, and the movement of the pull plate can drive the second rack to move; in summary, the transmission assembly set up is convenient for driving the second rack to move.

[0021] Preferably, a buffer mechanism for buffering the detection body is installed at one end of the movable plate away from the driving cylinder, and the buffer mechanism includes a fixed plate fixed to the end of the movable plate away from the driving cylinder and a buffer pad fixed to the top of the fixed plate.

[0022] By adopting the above solution, the fixed plate and the buffer pad are provided, so that the detection body can be buffered when the detection body falls from the clamping plate.

[0023] The present application provides a detection method of a microfluidic detection device for pathogenic microorganisms, which adopts the following technical solution: A detection method of a microfluidic detection device for pathogenic microorganisms comprises the following steps: The first step is to clean the detection body: first place the detection body between the two clamping plates, and then drive the two clamping plates to move toward each other through the two clamping cylinders, so that the detection body can be clamped; then the driving cylinder drives the moving plate to move in the direction close to the detection body, and the moving plate moves in the direction close to the detection body, driving the air blowing pipe to move in the direction close to the detection body; furthermore, the moving plate moves to drive the horizontal plate to move, and at this time the horizontal plate drives the first driving plate to move downward under the action of the first inclined surface, and then the first driving plate drives the second driving plate to move under the action of the second inclined surface, and the second driving plate moves to drive the first rack to move, the first rack movement drives the first gear to rotate, the first gear rotation drives the first horizontal axis to rotate, the first horizontal axis rotation drives the first connecting plate to rotate, and the rotation of the first connecting plate can make the dust hood in a vertical state, so that the opening of the dust hood is set toward the air blowing pipe; furthermore, the moving plate moves to drive the first connecting rod to move, the first connecting rod movement drives the third sliding sleeve to move, and the third sliding sleeve movement drives the second connecting rod to move The second connecting rod moves, the second connecting rod moves to drive the pull plate to move, the pull plate moves to drive the second rack to move, the second rack moves to drive the second gear to rotate, the second gear rotates to drive the second horizontal axis to rotate, and the second horizontal axis rotates to drive the second connecting plate to rotate, so that the baffle is above the placement table; then start the fan and the negative pressure pump, and the detection body can be cleaned at this time; when the detection body is cleaned, first drive the moving plate to reset by the driving cylinder, the moving plate resets the dust hood and the baffle, and then the dust hood resets to make the dust in the dust hood pour into the ash box, which is convenient for dust treatment; next, the two clamping plates are driven to move back to back by the piston rods of the two clamping cylinders, so that the detection body can be loosened, and then preparations can be made for the next formal detection; the second step, detection: during the detection, the detection body is first placed on the placement table, and then the detection liquid enters at the inlet end of the microchannel, passes through each detection chip in turn, and flows out at the outlet end of the microchannel at the other end, thereby completing the detection of pathogenic microorganisms.

[0024] In summary, this application has the following beneficial effects: 1. First, place the detection body between the two clamping plates, and then use two clamping cylinders to drive the two clamping plates to move toward each other, so that the detection body can be clamped; then, the detection body is blown by the soot blowing component, and then the dust blown by the soot blowing component is absorbed by the dust suction component, so that the detection body can be cleaned; In summary, the cleaning mechanism is set up to facilitate the cleaning of the detection body; 2. When the movable plate moves, it can combine with the transmission assembly to drive the second rack to move, the second rack movement drives the second gear to rotate, the second gear rotation drives the second horizontal axis to rotate, and the second horizontal axis rotation drives the second connecting plate to rotate, so that the baffle plate can be placed above the placement table, thereby facilitating the reduction of dust falling onto the top of the placement table; in summary, the dust blocking mechanism provided can reduce dust falling onto the top of the placement table; 3. The fixed plate and the buffer pad are provided to buffer the detection body when the detection body falls from the clamping plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application; Figure 2 This is a structural schematic diagram highlighting the clamping assembly in an embodiment of the present application; Figure 3 This is a structural schematic diagram highlighting the sootblowing assembly in the embodiment of the present application; Figure 4 This is a structural schematic diagram highlighting the dust collection component in an embodiment of the present application; Figure 5 It is a structural schematic diagram highlighting the dust blocking mechanism in the embodiment of the present application.

[0026] Description of reference numerals: 1, frame; 11, placement table; 2, detection body; 21, microchannel; 22, detection chip; 3, clamping assembly; 31, first vertical plate; 32, connection frame; 33, clamping cylinder; 34, clamping plate; 35, protective layer; 4, soot blowing assembly; 41, through hole; 42, moving plate; 43, driving cylinder; 44, blowing pipe; 45, nozzle; 46, fan; 5, dust suction assembly; 51, second vertical plate; 52, horizontal plate; 521, first horizontal axis; 53, first connecting plate; 54, dust cover; 55, first gear; 56, first rack; 57, negative pressure member; 571, mounting groove; 572, negative pressure pump; 573, negative pressure pipe; 574, dust filter plate; 575, air outlet pipe; 6, driving member; 61, first guide rod; 611, first sliding sleeve; 612, first stopper; 613, first reset Spring; 62, first drive plate; 63, horizontal plate; 631, first inclined plane; 64, second guide rod; 641, second sleeve; 642, second stopper; 643, second return spring; 65, second drive plate; 651, second inclined plane; 7, ash holding member; 71, support plate; 711, slot; 72, ash receiving box; 721, plug plate; 8, buffer mechanism; 81, fixed plate; 82, buffer pad; 9, ash stopper; 91, fixed frame; 92, second horizontal axis; 921, second connecting plate; 93, stopper; 94, second gear; 95, second rack; 96, transmission assembly; 961, first connecting rod; 962, third sleeve; 963, third guide rod; 964, second connecting rod; 965, pull plate; 966, fourth sleeve; 967, fourth guide rod; 968, third stopper; 969, third return spring. DETAILED DESCRIPTION

[0027] The following is combined with Figure 1-5 This application is described in further detail.

[0028] The same parts are denoted by the same reference numerals. It should be noted that the words "front", "rear", "left", "right", "upper", "lower", "bottom" and "top" used in the following description refer to directions in the drawings, and the words "inner" and "outer" refer to directions toward or away from the geometric center of a specific component, respectively.

[0029] The present application discloses a microfluidic detection device for pathogenic microorganisms, such as Figure 1 As shown, it includes a frame 1, a placement table 11, a detection body 2, a microchannel 21 and a plurality of detection chips 22. A cleaning mechanism for cleaning the detection body 2 is installed on the frame 1.

[0030] like Figure 1 and Figure 2 As shown, the cleaning mechanism includes a clamping assembly 3, a soot blowing assembly 4 and a dust collecting assembly 5 installed on the top of the frame 1; the clamping assembly 3 includes a first vertical plate 31 installed on the top of the frame 1, a connecting frame 32 installed on the top of the first vertical plate 31, two clamping cylinders 33 respectively installed at both ends of the connecting frame 32, and two clamping plates 34 respectively installed on the piston rods of the two clamping cylinders 33; the two clamping plates 34 are used to clamp the detection body 2, and a protective layer 35 is fixed on one side of each clamping plate 34. First, the detection body 2 is placed between the two clamping plates 34, and then the two clamping cylinders 33 drive the two clamping plates 34 to move toward each other, so that the detection body 2 can be clamped; then the soot blowing assembly 4 is used to blow soot on the detection body 2, and then the dust blown off by the soot blowing assembly 4 is absorbed by the dust collecting assembly 5, so that the detection body 2 can be cleaned; in summary, the cleaning mechanism is set up to facilitate the cleaning of the detection body 2.

[0031] like Figure 1 and Figure 3 As shown, the soot blowing assembly 4 includes a through hole 41 opened in the first vertical plate 31, a movable plate 42 slidably connected to the through hole 41 along the length direction of the frame 1, and a driving cylinder 43 horizontally installed on one side of the first vertical plate 31 for driving the movable plate 42 to move; a blowing pipe 44 is vertically installed on the top of the movable plate 42, and the two ends of the blowing pipe 44 are closed. The blowing pipe 44 is connected to a plurality of nozzles 45 in sequence along the vertical direction on the side close to the clamping plate 34, and the nozzles 45 are inclined toward the detection body 2. The other side of the blowing pipe 44 is connected to a fan 46. When the detection body 2 needs to be soot-blown, the piston rod of the driving cylinder 43 is first used to drive the movable plate 42 to move in the direction close to the detection body 2, and the movable plate 42 moves in the direction close to the detection body 2 to drive the blowing pipe 44 to move in the direction close to the detection body 2, and then the fan 46 is started, and the detection body 2 can be soot-blown through the nozzles 45 at this time; in summary, the soot-blowing assembly 4 is provided to facilitate soot-blowing of the detection body 2.

[0032] like Figure 1 and Figure 4 As shown, the dust collection assembly 5 includes a second vertical plate 51 vertically fixed to the top of the frame 1, a horizontal plate 52 horizontally installed on one side of the second vertical plate 51, a first horizontal shaft 521 horizontally rotatably connected to the horizontal plate 52 away from the second vertical plate 51 through a bearing, a first connecting plate 53 fixed to the first horizontal shaft 521, a dust collection cover 54 installed at the end of the first connecting plate 53 away from the first horizontal shaft 521, a negative pressure member 57 installed in the dust collection cover 54, and a The first gear 55 and the first rack 56 meshed with the first gear 55; the first rack 56 and the dust hood 54 are staggered so as not to affect the rotation of the dust hood 54. A driving member 6 for driving the first rack 56 to move is installed on the horizontal plate 52, and an ash holding member 7 is installed on one side of the second vertical plate 51; in the initial state, the opening of the dust hood 54 is downward, so that the ash holding member 7 can hold ash conveniently, and the driving member 6 can make the opening of the dust hood 54 face the ash blowing assembly 4, so as to facilitate ash suction. When the movable plate 42 moves, it can combine with the driving member 6 to drive the first rack 56 to move, the first rack 56 moves to drive the first gear 55 to rotate, the first gear 55 rotates to drive the first horizontal axis 521 to rotate, the first horizontal axis 521 rotates to drive the first connecting plate 53 to rotate, and the rotation of the first connecting plate 53 can make the dust hood 54 in a vertical state; then start the negative pressure member 57, and at this time the dust blown off by the soot blowing assembly 4 can be absorbed; in summary, the dust collection assembly 5 is set to facilitate the absorption of dust blown off by the soot blowing assembly 4.

[0033] like Figure 1 and Figure 4 As shown, the negative pressure member 57 includes a mounting groove 571 provided in the dust cover 54 and a plurality of negative pressure pumps 572 installed in the mounting groove 571; a negative pressure pipe 573 is installed at the inlet of the negative pressure pump 572, a dust blocking filter plate 574 is installed in the dust cover 54, and one end of the negative pressure pipe 573 away from the negative pressure pump 572 penetrates through one side wall of the mounting groove 571 and is arranged toward the dust blocking filter plate 574; the outlet of the negative pressure pump 572 is connected to an air outlet pipe 575, and one end of the air outlet pipe 575 away from the negative pressure pump 572 penetrates through the side wall of the mounting groove 571. The negative pressure pump 572 and the negative pressure pipe 573 are provided to facilitate the dust cover 54 to generate suction; the dust blocking filter plate 574 is provided to block dust.

[0034] like Figure 1 and Figure 4As shown, the driving member 6 includes a first guide rod 61 fixedly connected to the top of the horizontal plate 52, a first sleeve 611 vertically slidably connected to the first guide rod 61, a first stopper 612 fixedly connected to the side wall of the first guide rod 61, and a first return spring 613 vertically arranged between the first stopper 612 and the first sleeve 611; a first driving plate 62 is vertically fixedly connected to one side of the first sleeve 611, a horizontal plate 63 is horizontally arranged on the top of the first driving plate 62, one end of the horizontal plate 63 away from the first driving plate 62 is fixedly connected to the outer wall of the blowing pipe 44, and first inclined surfaces 631 are respectively arranged on the opposite inner sides of the first driving plate 62 and the horizontal plate 63, and the two first inclined surfaces 631 are respectively arranged. 31 matches; the driving member 6 also includes a second guide rod 64 fixedly connected to one side of the cross plate 52, a second sleeve 641 slidably connected to the second guide rod 64 in the horizontal direction, a second stopper 642 fixedly connected to the side wall of the second guide rod 64, and a second return spring 643 horizontally arranged between the second stopper 642 and the second sleeve 641; a second driving plate 65 is fixedly connected to one side of the second sleeve 641, and second inclined surfaces 651 are respectively arranged on the opposite inner sides of the first driving plate 62 and the second driving plate 65, and the two second inclined surfaces 651 match each other; one end of the first rack 56 away from the first gear 55 is fixedly connected to the end of the second driving plate 65 away from the first driving plate 62. The movable plate 42 moves and drives the horizontal plate 63 to move. At this time, the horizontal plate 63 drives the first driving plate 62 to move downward under the action of the first inclined surface 631, and then the first driving plate 62 drives the second driving plate 65 to move under the action of the second inclined surface 651. The second driving plate 65 moves and drives the first rack 56 to move. The first rack 56 moves and drives the first gear 55 to rotate. The first gear 55 rotates and drives the first horizontal axis 521 to rotate. The first horizontal axis 521 rotates and drives the first connecting plate 53 to rotate. The rotation of the first connecting plate 53 can make the dust hood 54 be in a vertical state. In summary, the driving member 6 is set to facilitate the dust hood 54 to be in a vertical state.

[0035] like Figure 1 As shown, the ash receiving member 7 includes a support plate 71 horizontally fixed to the side wall of the second vertical plate 51 and an ash receiving box 72 plugged into the top of the support plate 71; a plug plate 721 is fixed to the bottom of the ash receiving box 72, a slot 711 is provided on the top of the support plate 71, and the plug plate 721 is plugged into the slot 711. The ash receiving box 72 is provided to facilitate receiving dust falling from the dust collecting hood 54.

[0036] like Figure 1 and Figure 3 As shown, a buffer mechanism 8 for buffering the detection body 2 is installed at one end of the moving plate 42 away from the driving cylinder 43, and the buffer mechanism 8 includes a fixed plate 81 horizontally fixed to one end of the moving plate 42 away from the driving cylinder 43 and a buffer pad 82 fixed to the top of the fixed plate 81. The fixed plate 81 and the buffer pad 82 are arranged to facilitate buffering the detection body 2 when the detection body 2 falls from the clamping plate 34.

[0037] like Figure 1 and Figure 5 As shown, a dust blocking mechanism 9 for blocking dust from the top of the placement table 11 is installed on the top of the frame 1. The dust blocking mechanism 9 includes a fixed frame 91 fixed to the top of the frame 1, a second horizontal axis 92 horizontally rotatably connected to the top of the fixed frame 91 through a bearing, a second connecting plate 921 fixed to the second horizontal axis 92, and a baffle 93 fixed to the second connecting plate 921 away from the end of the second horizontal axis 92; a second gear 94 is sleeved and fixed on the second horizontal axis 92, and a second rack 95 is meshed on the second gear 94. The dust blocking mechanism 9 also includes a transmission assembly 96 for driving the second rack 95 to move; in the initial state, the baffle 93 is in a vertical state, which is convenient for placing the detection body 2 on the top of the placement table 11 for detection, and the transmission assembly 96 can make the baffle 93 horizontal and on the top of the placement table 11, which is convenient for blocking dust from the top of the placement table 11. When the movable plate 42 moves, it can combine with the transmission assembly 96 to drive the second rack 95 to move, the second rack 95 moves to drive the second gear 94 to rotate, the second gear 94 rotates to drive the second horizontal axis 92 to rotate, and the second horizontal axis 92 rotates to drive the second connecting plate 921 to rotate, so that the baffle 93 can be located above the placing table 11, thereby facilitating the reduction of dust falling to the top of the placing table 11; in summary, the dust blocking mechanism 9 set up can reduce dust falling to the top of the placing table 11.

[0038] like Figure 1 and Figure 5 As shown, the transmission assembly 96 includes a first connecting rod 961 hinged to one end of the moving plate 42 and a third sliding sleeve 962 hinged to the end of the first connecting rod 961 away from the moving plate 42; a third guide rod 963 is fixedly connected to one side of the first vertical plate 31, and the third sliding sleeve 962 is vertically slidably connected to the third guide rod 963; a second connecting rod 964 is hinged to the bottom of the third sliding sleeve 962, and the end of the second connecting rod 964 away from the third sliding sleeve 962 is hinged to the pull plate 9 65, one end of the pull plate 965 away from the second connecting rod 964 is fixed to one end of the second rack 95; the bottom of the pull plate 965 is fixed with a fourth sliding sleeve 966, the top of the fixed frame 91 is fixed with a fourth guide rod 967, the fourth sliding sleeve 966 is slidably connected to the fourth guide rod 967 in the horizontal direction, one end of the fourth guide rod 967 is fixed with a third stopper 968, and a third return spring 969 is horizontally arranged between the third stopper 968 and the fourth sliding sleeve 966. The movement of the moving plate 42 drives the first connecting rod 961 to move, the movement of the first connecting rod 961 drives the movement of the third sliding sleeve 962, the movement of the third sliding sleeve 962 drives the movement of the second connecting rod 964, the movement of the second connecting rod 964 drives the pull plate 965 to move, and the movement of the pull plate 965 can drive the second rack 95 to move; in summary, the transmission assembly 96 is arranged to facilitate the movement of the second rack 95.

[0039] The present application discloses a detection method of a microfluidic detection device for pathogenic microorganisms, comprising the following steps: The first step is to clean the detection body 2: first, place the detection body 2 between the two clamping plates 34, and then drive the two clamping plates 34 to move toward each other through the two clamping cylinders 33, so that the detection body 2 can be clamped; then drive the cylinder 43 to drive the moving plate 42 to move in the direction close to the detection body 2, and the moving plate 42 moves in the direction close to the detection body 2 to drive the blowing pipe 44 to move in the direction close to the detection body 2; then, the moving plate 42 moves to drive the horizontal plate 63 to move, and at this time, the horizontal plate 63 is driven by the first inclined surface 631. The first driving plate 62 is driven to move downward, and then the first driving plate 62 drives the second driving plate 65 to move under the action of the second inclined surface 651, the second driving plate 65 moves to drive the first rack 56 to move, the first rack 56 moves to drive the first gear 55 to rotate, the first gear 55 rotates to drive the first horizontal shaft 521 to rotate, the first horizontal shaft 521 rotates to drive the first connecting plate 53 to rotate, the first connecting plate 53 rotates to make the dust cover 54 in a vertical state, so that the opening of the dust cover 54 is set toward the blowing pipe 44; furthermore, the moving plate 42 moves The first connecting rod 961 is driven to move, the first connecting rod 961 drives the third sliding sleeve 962 to move, the third sliding sleeve 962 moves to drive the second connecting rod 964 to move, the second connecting rod 964 drives the pull plate 965 to move, the pull plate 965 drives the second rack 95 to move, the second rack 95 drives the second gear 94 to rotate, the second gear 94 rotates to drive the second horizontal shaft 92 to rotate, the second horizontal shaft 92 rotates to drive the second connecting plate 921 to rotate, so that the baffle 93 can be placed above the placement table 11; then the fan 4 is started 6 and the negative pressure pump 572, the detection body 2 can be cleaned at this time; when the detection body 2 is cleaned, the moving plate 42 is first driven to reset by the driving cylinder 43, and the reset of the moving plate 42 resets the dust hood 54 and the baffle 93, and then the reset of the dust hood 54 can make the dust in the dust hood 54 pour into the dust box 72, which is convenient for dust disposal; next, the piston rods of the two clamping cylinders 33 drive the two clamping plates 34 to move back to each other, so that the detection body 2 can be loosened, and then preparations can be made for the next formal test; Step 2, detection: During detection, the detection body 2 is first placed on the placement table 11, and then the detection liquid enters the inlet end of the microchannel 21, passes through each detection chip 22 in turn, and flows out from the outlet end of the microchannel 21 at the other end, thereby completing the detection of pathogenic microorganisms.

[0040] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A microfluidic detection device for pathogenic microorganisms, comprising a frame (1), a placement table (11), a detection body (2), a microchannel (21) and a plurality of detection chips (22), characterized in that: The frame (1) is provided with a cleaning mechanism for cleaning the detection body (2), the cleaning mechanism comprising a clamping assembly (3) mounted on the top of the frame (1), a soot blowing assembly (4) and a dust collecting assembly (5); the clamping assembly (3) comprises a first vertical plate (31) mounted on the top of the frame (1), a connecting frame (32) mounted on the top of the first vertical plate (31), two clamping cylinders (33) respectively mounted on both ends of the connecting frame (32), and two clamping plates (34) respectively mounted on the piston rods of the two clamping cylinders (33); the two clamping plates (34) are used to clamp the detection body (2), and a protective layer (35) is fixedly connected to one side of each clamping plate (34).

2. A microfluidic detection device for pathogenic microorganisms according to claim 1, characterized in that: The soot blowing assembly (4) comprises a through hole (41) opened in the first vertical plate (31), a movable plate (42) slidably connected to the through hole (41), and a driving cylinder (43) installed on one side of the first vertical plate (31) for driving the movable plate (42) to move; an air blowing pipe (44) is installed on the top of the movable plate (42); a side of the air blowing pipe (44) close to the clamping plate (34) is connected to a plurality of nozzles (45), the nozzles (45) are arranged obliquely toward the detection body (2), and the other side of the air blowing pipe (44) is connected to a fan (46).

3. A microfluidic detection device for pathogenic microorganisms according to claim 2, characterized in that: The dust collecting assembly (5) comprises a second vertical plate (51) fixedly connected to the top of the frame (1), a horizontal plate (52) installed on one side of the second vertical plate (51), a first horizontal axis (521) rotatably connected to an end of the horizontal plate (52) away from the second vertical plate (51), a first connecting plate (53) fixedly connected to the first horizontal axis (521), a dust collecting cover (54) installed on an end of the first connecting plate (53) away from the first horizontal axis (521), a negative pressure member (57) installed in the dust collecting cover (54), and a first connecting plate (53) fixedly connected to the first horizontal axis (521). A first gear (55) of the horizontal shaft (521) and a first rack (56) meshed with the first gear (55); a driving member (6) for driving the first rack (56) to move is installed on the horizontal plate (52), and an ash holding member (7) is installed on one side of the second vertical plate (51); in the initial state, the opening of the dust hood (54) is downward, which is convenient for the ash holding member (7) to hold ash, and the driving member (6) can make the opening of the dust hood (54) face the ash blowing assembly (4), which is convenient for ash suction.

4. A microfluidic detection device for pathogenic microorganisms according to claim 3, characterized in that: The negative pressure component (57) includes a mounting groove (571) provided in the dust hood (54) and a plurality of negative pressure pumps (572) installed in the mounting groove (571); a negative pressure pipe (573) is installed at the inlet of the negative pressure pump (572), a dust filter plate (574) is installed in the dust hood (54), and the end of the negative pressure pipe (573) away from the negative pressure pump (572) passes through a side wall of the mounting groove (571) and is arranged toward the dust filter plate (574); the outlet of the negative pressure pump (572) is connected to an air outlet pipe (575), and the end of the air outlet pipe (575) away from the negative pressure pump (572) passes through the side wall of the mounting groove (571).

5. The microfluidic detection device for pathogenic microorganisms according to claim 3, characterized in that: The driving member (6) comprises a first guide rod (61) fixedly connected to the top of the transverse plate (52), a first sliding sleeve (611) slidably connected to the first guide rod (61), a first stopper (612) fixedly connected to the side wall of the first guide rod (61), and a first return spring (613) arranged between the first stopper (612) and the first sliding sleeve (611); a first driving plate (62) is fixedly connected to one side of the first sliding sleeve (611), a horizontal plate (63) is arranged on the top of the first driving plate (62), an end of the horizontal plate (63) away from the first driving plate (62) is fixedly connected to the outer wall of the blowing pipe (44), and first inclined surfaces (631) are respectively arranged on the opposite inner sides of the first driving plate (62) and the horizontal plate (63), and the two first inclined surfaces (631) are arranged on the inner sides of the first driving plate (62) and the horizontal plate (63). ) match; the driving member (6) also includes a second guide rod (64) fixedly connected to one side of the cross plate (52), a second sliding sleeve (641) slidably connected to the second guide rod (64), a second stopper (642) fixedly connected to the side wall of the second guide rod (64), and a second return spring (643) arranged between the second stopper (642) and the second sliding sleeve (641); a second driving plate (65) is fixedly connected to one side of the second sliding sleeve (641), and second inclined surfaces (651) are respectively arranged on the opposite inner sides of the first driving plate (62) and the second driving plate (65), and the two second inclined surfaces (651) match each other; one end of the first rack (56) away from the first gear (55) is fixedly connected to one end of the second driving plate (65) away from the first driving plate (62).

6. The microfluidic detection device for pathogenic microorganisms according to claim 3, characterized in that: The ash receiving member (7) comprises a support plate (71) fixedly connected to the side wall of the second vertical plate (51) and an ash receiving box (72) plugged into the top of the support plate (71); a plugging plate (721) is fixedly connected to the bottom of the ash receiving box (72), a slot (711) is provided on the top of the support plate (71), and the plugging plate (721) is plugged into the slot (711).

7. A microfluidic detection device for pathogenic microorganisms according to claim 2, characterized in that: A dust shielding mechanism (9) for shielding dust from the top of the placement table (11) is installed on the top of the frame (1), and the dust shielding mechanism (9) comprises a fixing frame (91) fixedly connected to the top of the frame (1), a second horizontal shaft (92) rotatably connected to the top of the fixing frame (91), a second connecting plate (921) fixedly connected to the second horizontal shaft (92), and a shielding plate (93) fixedly connected to the end of the second connecting plate (921) away from the second horizontal shaft (92); a second gear is sleeved and fixed on the second horizontal shaft (92). (94), a second rack (95) is meshed on the second gear (94), and the dust blocking mechanism (9) also includes a transmission assembly (96) for driving the second rack (95) to move; in the initial state, the baffle (93) is in a vertical state, which is convenient for placing the detection body (2) on the top of the placement table (11) for detection, and the transmission assembly (96) can make the baffle (93) horizontal and on the top of the placement table (11), which is convenient for blocking dust on the top of the placement table (11).

8. The microfluidic detection device for pathogenic microorganisms according to claim 7, characterized in that: The transmission assembly (96) comprises a first connecting rod (961) hinged to one end of the movable plate (42) and a third sliding sleeve (962) hinged to one end of the first connecting rod (961) away from the movable plate (42); a third guide rod (963) is fixedly connected to one side of the first vertical plate (31), and the third sliding sleeve (962) is slidably connected to the third guide rod (963); a second connecting rod (964) is hinged to the bottom of the third sliding sleeve (962), and the end of the second connecting rod (964) away from the third sliding sleeve (962) is hinged to the pull plate (96 5), one end of the pull plate (965) away from the second connecting rod (964) is fixed to one end of the second rack (95); the bottom of the pull plate (965) is fixed with a fourth sliding sleeve (966), the top of the fixed frame (91) is fixed with a fourth guide rod (967), the fourth sliding sleeve (966) is slidably connected to the fourth guide rod (967), one end of the fourth guide rod (967) is fixed with a third stopper (968), and a third return spring (969) is arranged between the third stopper (968) and the fourth sliding sleeve (966).

9. The microfluidic detection device for pathogenic microorganisms according to claim 2, characterized in that: A buffer mechanism (8) for buffering the detection body (2) is installed at one end of the movable plate (42) away from the driving cylinder (43), and the buffer mechanism (8) comprises a fixed plate (81) fixedly connected to one end of the movable plate (42) away from the driving cylinder (43) and a buffer pad (82) fixedly connected to the top of the fixed plate (81).

10. A method for detecting pathogenic microorganisms using a microfluidic detection device, based on the microfluidic detection device for pathogenic microorganisms according to any one of claims 1 to 9, characterized in that: The following steps are involved: The first step is to clean the detection body (2): first, the detection body (2) is placed between the two clamping plates (34), and then the two clamping cylinders (33) are used to drive the two clamping plates (34) to move toward each other, so that the detection body (2) can be clamped; then the cylinder (43) is driven to drive the moving plate (42) to move in the direction close to the detection body (2), and the moving plate (42) moves in the direction close to the detection body (2) to drive the blowing pipe (44) to move in the direction close to the detection body (2); further, the moving plate (42) moves to drive the horizontal plate (63) to move, and at this time, the horizontal plate (63) is under the action of the first inclined surface (631). The first driving plate (62) is driven to move downward, and then the first driving plate (62) drives the second driving plate (65) to move under the action of the second inclined surface (651), the second driving plate (65) moves to drive the first rack (56) to move, the first rack (56) moves to drive the first gear (55) to rotate, the first gear (55) rotates to drive the first horizontal axis (521) to rotate, the first horizontal axis (521) rotates to drive the first connecting plate (53) to rotate, the first connecting plate (53) rotates to make the dust cover (54) in a vertical state, so that the opening of the dust cover (54) is arranged toward the air blowing pipe (44); furthermore, the moving plate (42 ) moves to drive the first connecting rod (961) to move, the first connecting rod (961) moves to drive the third sliding sleeve (962) to move, the third sliding sleeve (962) moves to drive the second connecting rod (964) to move, the second connecting rod (964) moves to drive the pull plate (965) to move, the pull plate (965) moves to drive the second rack (95) to move, the second rack (95) moves to drive the second gear (94) to rotate, the second gear (94) rotates to drive the second horizontal shaft (92) to rotate, the second horizontal shaft (92) rotates to drive the second connecting plate (921) to rotate, so that the baffle (93) can be placed above the placement table (11); then start The fan (46) and the negative pressure pump (572) are driven, and the detection body (2) can be cleaned at this time; when the detection body (2) is cleaned, the movable plate (42) is first driven to reset by the driving cylinder (43), and the movable plate (42) is reset so that the dust hood (54) and the baffle (93) are both reset, and then the dust hood (54) is reset so that the dust in the dust hood (54) can be poured into the dust box (72), so that the dust can be easily processed; next, the piston rods of the two clamping cylinders (33) drive the two clamping plates (34) to move in opposite directions, so that the detection body (2) can be loosened, and then preparations can be made for the next formal test; Step 2: Detection: During the detection, the detection body (2) is first placed on the placement table (11), and then the detection liquid enters the inlet end of the microchannel (21), passes through each detection chip (22) in turn, and flows out from the outlet end of the microchannel (21) at the other end, thereby completing the detection of pathogenic microorganisms.

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