Multi-needle picking head independent drive control device for high-throughput bacterial colony screening and application of multi-needle picking head independent drive control device

Abstract

The invention provides a multi-needle picking head independent driving control device for high-throughput bacterial colony screening, which comprises an X-direction valve block arranged along the Y-axis direction of a gas circuit board and a Y-direction valve block arranged along the X-axis direction of the gas circuit board, and the Y-direction valve block is provided with a cylinder driving control gas inlet; a plurality of valve element switching control valves are arranged on the X-direction valve block at intervals, a valve element switching control air inlet is formed in the X-direction valve block, and a plurality of control valve elements are arranged in the air path plate. The Y-direction valve block is provided with a plurality of air cylinder driving control valves, an air cylinder control hole is formed in the air cylinder plate corresponding to the X-direction coordinates of each group of valve element switching control valves and the Y-direction coordinates of the air cylinder driving control valves, an air cylinder is installed on each air cylinder control hole, and the air cylinder driving control valves and the valve element switching control valves are electrically connected with a central control system. Power-on and power-off of the electromagnetic coil of the control valve are switched through the control valve element, the multiple needle picking heads are independently driven to operate, and then the action of picking bacterial colonies is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial experimental equipment, and in particular relates to a multi-needle picking head independent drive control device and method for high-throughput colony screening. Background Art

[0002] In modern microbial research, high-throughput colony screening is a key link in obtaining target strains. Traditional colony screening equipment has many shortcomings in its picking needle and picking head design. On the one hand, the number of picking needles is limited, which makes it difficult to meet the needs of rapid screening of large-scale colony samples, resulting in low screening efficiency. On the other hand, most of the existing picking needles cannot be controlled independently and can only be operated in batches or picked with a single needle. The batch operation needle array cannot accurately pick and inoculate a single colony, and the single-needle operation screening and picking efficiency is low, which seriously affects the accuracy of the screening results and the screening efficiency. Therefore, it is of great practical significance to develop a system that can realize independent drive of an array-type multi-needle picking head and is suitable for high-throughput colony screening. Summary of the invention

[0003] In view of this, the present invention aims to overcome the defects in the prior art and proposes an independent drive control device and method for a multi-needle picking head for high-throughput colony screening, so as to realize independent drive operation of the multi-needle picking heads, enable the central control system to accurately control the drive control system and control the cylinder movement behind any picking needle, and enable the picking needle to move independently to realize the action of colony picking, thereby significantly improving the efficiency and accuracy of high-throughput colony screening.

[0004] To achieve the above object, the technical solution created by the present invention is implemented as follows:

[0005] A multi-needle pick independent drive control device for high-throughput colony screening comprises an X-direction valve block arranged along the Y-axis direction of an air circuit board, and a Y-direction valve block arranged along the X-axis direction of the air circuit board, wherein a cylinder drive control air inlet is provided on the Y-direction valve block; a plurality of valve core switching control valves are arranged at intervals on the X-direction valve block, and a valve core switching control air inlet is provided on the X-direction valve block, a plurality of control valve cores are provided in the air circuit board, and the control valve cores and the valve core switching control valves are arranged one by one; a plurality of cylinder drive control valves are arranged at intervals on the Y-direction valve block, and a cylinder control hole is provided on the cylinder plate corresponding to the X-direction coordinate of each group of valve core switching control valves and the Y-direction coordinate of the cylinder drive control valve, and a cylinder is installed on each cylinder control hole, and each cylinder drive control valve and each valve core switching control valve are electrically connected to a central control system.

[0006] Furthermore, the valve core switching control valve adopts a solenoid valve, and the cylinder driving control valve adopts a solenoid valve.

[0007] Furthermore, each cylinder is arranged vertically.

[0008] Furthermore, the cylinder valve core switching control air inlet is installed on the air inlet joint.

[0009] Furthermore, the cylinder drive control air inlet is installed on the air inlet joint.

[0010] Furthermore, the air paths corresponding to the cylinder drive control valves are arranged in parallel with each other.

[0011] A method for performing point-to-point high-speed screening of colonies using the multi-needle picking head independent drive control device for high-throughput colony screening comprises the following steps:

[0012] Combined with the matrix coordinates of the needle picking, the central control system is used to calculate the action combination of the X-axis and Y-axis control valves that need to be controlled;

[0013] The central control system sends control signals to the corresponding control valves to control the needle picking action;

[0014] Combined with the central control system monitoring device, the working status and position information of the needle picker can be monitored in real time.

[0015] Furthermore, the central control system includes an industrial computer.

[0016] Compared with the prior art, the present invention has the following advantages:

[0017] The invention provides a multi-needle picking head independent drive control device for high-throughput colony screening. When used, the electromagnetic coil of the control valve is switched on and off by controlling the valve core to realize the drive control of each picking needle. When the electromagnetic coil of a certain control valve is energized, the corresponding electromagnetic valve is opened to drive the corresponding needle picking action, and through the action combination of the X-axis and Y-axis control valves, the multi-needle picking heads are driven and operated independently, so that the central control system can accurately control the drive control system and control the cylinder action behind any needle picking to realize the colony picking action, thereby significantly improving the efficiency and accuracy of high-throughput colony screening. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings constituting part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments and descriptions of the present invention are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0019] Figure 1 A schematic diagram of the structure created by the present invention;

[0020] Figure 2 A schematic diagram that creates another perspective for the present invention;

[0021] Figure 3 This is a schematic diagram of the control valve core in the closed state in the invention;

[0022] Figure 4 This is a schematic diagram of the control valve core being connected to the cylinder in the invention;

[0023] Figure 5 The present invention provides a schematic diagram of the structure arrangement of the valve core switching control valve and the cylinder driven control valve;

[0024] Figure 6 It is a schematic diagram of the distribution of cylinder control holes in the invention. DETAILED DESCRIPTION

[0025] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0026] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0027] In the description of the invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the invention can be understood according to specific circumstances.

[0028] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0029] A multi-needle pick independent drive control device for high-throughput colony screening, such as Figures 1 to 6As shown, it includes a gas circuit board 1 and a cylinder board 2. The gas circuit board is provided with an X-direction valve block 3 along the Y-axis direction and a Y-direction valve block 4 along the X-axis direction. A plurality of valve core switching control valves 5 are arranged at intervals on the X-direction valve block, and a valve core switching control air inlet 6 is provided on the X-direction valve block. In order to facilitate the arrangement of the gas circuit on the gas circuit board, the Y-direction valve block includes a first valve block split 8 and a second valve block split 9 arranged correspondingly on both sides of the gas circuit board. The first valve block split and the second valve block split are both provided with a cylinder drive control air inlet. The cylinder drive control air inlet and the valve core switching control air inlet are both installed on the air inlet joint.

[0030] A control valve core 10 is provided for each valve core switching control valve in the gas circuit board, and each valve core is arranged in gas circuits parallel to each other. A plurality of cylinder drive control valves 7 are provided at intervals on the Y-direction valve block, and a cylinder drive control air inlet 13 is provided on the Y-direction valve block. The combination of the horizontal control valve and the longitudinal control valve can realize the connection of compressed air to the corresponding drive point, thereby realizing independent control of the needle picking at any position in the matrix. As an example, there are 96 drive points on the gas circuit board, and 6 groups of cylinder drive control valves are provided on the first valve block split and the second valve block split, and 8 groups of valve core switching control valves are provided on the X-direction valve block. The arrangement and spacing are the same as those of the 96-well plate commonly used in biological laboratories.

[0031] The cylinder plate is provided with a cylinder control hole 11 at the driving point determined by the X-axis coordinate of any valve core switching control valve and the Y-axis coordinate of any cylinder drive control valve, and each cylinder control hole is installed with a cylinder 12 for driving the needle picking action. Each cylinder is arranged vertically, and the cylinder adopts a micro cylinder. Each cylinder drive control valve and each valve core switching control valve are electrically connected to the central control system. As an example, the valve core switching control valve adopts a solenoid valve. The cylinder drive control valve adopts a solenoid valve.

[0032] When in use, the solenoid coil of the control valve is switched on and off by controlling the valve core to realize the drive control of each picking needle. External compressed air is used as the air source, and the compressed air is transported to each cylinder by the air circuit. Each cylinder corresponds to a picking needle, and the piston rod of the cylinder is connected to the picking needle. By controlling the air pressure change in the cylinder, the linear motion of the picking needle is realized. As an example, in order to ensure the stability and precise control of the air circuit, components such as pressure regulating valves and flow control valves can be set in the air circuit.

[0033] A method for performing point-to-point high-speed screening of colonies using the multi-needle picking head independent drive control device for high-throughput colony screening comprises the following steps:

[0034] Combined with the matrix coordinates of the needle picking, the central control system is used to calculate the action combination of the X-axis and Y-axis control valves that need to be controlled;

[0035] The central control system sends control signals to the corresponding control valves to control the needle picking action;

[0036] Combined with the central control system monitoring device, the working status and position information of the needle picker can be monitored in real time.

[0037] The central control system includes an industrial computer.

[0038] The position of each needle is represented by coordinates

[0039]

[0040] Inlet 1 supplies air to solenoid valves A, B, C, D, E, F, G, and H; inlet 2 supplies air to solenoid valves 2, 4, 6, 8, 10, and 12; inlet 3 supplies air to solenoid valves 1, 3, 5, 7, 9, and 11. The three air inlets A, B, and C work together to control the corresponding needle movements.

[0041] How it works

[0042] To extend the (A, 1) needle, first take in three inlets of air, solenoid valve 1 opens, then take in one inlet of air, solenoid valve A opens.

[0043] The invention provides a multi-needle picking head independent drive control device for high-throughput colony screening. When used, the electromagnetic coil of the control valve is switched on and off by controlling the valve core to realize the drive control of each picking needle. When the electromagnetic coil of a certain control valve is energized, the corresponding electromagnetic valve is opened to drive the corresponding needle picking action, and through the action combination of the X-axis and Y-axis control valves, the multi-needle picking heads are driven and operated independently, so that the central control system can accurately control the drive control system and control the cylinder action behind any needle picking to realize the colony picking action, thereby significantly improving the efficiency and accuracy of high-throughput colony screening.

[0044] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A multi-needle pick independent drive control device for high-throughput colony screening, characterized in that: It comprises an X-direction valve block arranged along the Y-axis direction of the gas circuit board, and a Y-direction valve block arranged along the X-axis direction of the gas circuit board, and a cylinder drive control air inlet is arranged on the Y-direction valve block; a plurality of valve core switching control valves are arranged at intervals on the X-direction valve block, and a valve core switching control air inlet is arranged on the X-direction valve block, and a plurality of control valve cores are arranged in the gas circuit board, and the control valve cores and the valve core switching control valves are arranged one by one; a plurality of cylinder drive control valves are arranged at intervals on the Y-direction valve block, and a cylinder control hole is arranged on the cylinder plate corresponding to the X-direction coordinate of each group of valve core switching control valves and the Y-direction coordinate of the cylinder drive control valve, and a cylinder is installed on each cylinder control hole, and each cylinder drive control valve and each valve core switching control valve are electrically connected to the central control system.

2. The multi-needle picking head independent drive control device for high-throughput colony screening according to claim 1, characterized in that: The valve core switching control valve adopts a solenoid valve, and the cylinder driving control valve adopts a solenoid valve.

3. The multi-needle picking head independent drive control device for high-throughput colony screening according to claim 1, characterized in that: Each cylinder is arranged vertically.

4. The multi-needle picking head independent drive control device for high-throughput colony screening according to claim 1, characterized in that: The cylinder valve core switching control air inlet is installed on the air inlet connector.

5. The multi-needle picking head independent drive control device for high-throughput colony screening according to claim 1, characterized in that: The cylinder drive control air inlet is installed on the air inlet connector.

6. The multi-needle picking head independent drive control device for high-throughput colony screening according to claim 1, characterized in that: The gas paths corresponding to the cylinder drive control valves are arranged in parallel with each other.

7. A method for performing point-to-point high-speed screening of colonies using the multi-needle pick independent drive control device for high-throughput colony screening according to any one of claims 1 to 6, characterized in that: The steps include: Combined with the matrix coordinates of the needle picking, the central control system is used to calculate the action combination of the X-axis and Y-axis control valves that need to be controlled; The central control system sends control signals to the corresponding control valves to control the needle picking action; Combined with the central control system monitoring device, the working status and position information of the needle picker can be monitored in real time.

8. A method for performing point-to-point high-speed screening of colonies using a multi-needle picking head independent drive control device for high-throughput colony screening according to claim 7, characterized in that: The central control system includes an industrial computer.

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