Novel microsphere culture device

Abstract

The invention relates to the related technical field of cell culture, in particular to a novel microsphere culture device which comprises a box body, a plate body is fixed to the inner side wall of the box body, a sliding groove plate is slidably connected to the inner side wall of the plate body, a shelf is arranged on one side of the sliding groove plate, and a height adjusting assembly is arranged on the inner side wall of the plate body. The height adjusting assembly comprises synchronizing wheels II which are symmetrically and rotationally connected to the inner side wall of the plate body; the synchronizing wheel I is rotationally connected to the inner side wall of the plate body; the multiple shelves are driven to move upwards or downwards through transmission of the synchronous belt, the distance between the shelves and the box body can be conveniently and rapidly adjusted, the positions of the partition plates on the synchronous belt can be directly and manually controlled by releasing the restraining effect of the limiting assembly and controlling the synchronous belt to work again, the distance between the shelves can be conveniently and rapidly adjusted, and the operation is convenient. Therefore, the distance between the partition plates can be conveniently adjusted according to the needs of users for placing different culture containers.

Description

Technical Field

[0001] The present invention relates to the technical field related to cell culture, and in particular to a novel microsphere culture device. Background Art

[0002] Cell microsphere culture is a core technology that simulates the in vivo microenvironment through a three-dimensional dynamic environment. Its core lies in the coordinated optimization of customized microcarrier design and dynamic control system. Compared with traditional two-dimensional culture, microspheres support cell attachment and proliferation through high specific surface area (such as agar microspheres with 200 times amplification efficiency) and biomimetic structure (such as the ECM coating of biomimetic Niche microcarriers). At the same time, combined with multi-mode controllable bioreactors (such as oscillation and rotation), the shear force is reduced to below 0.1 Pa to avoid mechanical stress damage to stem cells.

[0003] The cell carbon dioxide incubator is a kind of incubator that precisely controls the temperature (37°C), humidity (95%), CO 2 The core technology includes infrared sensor (IR) or thermal conductivity sensor (TC) to monitor CO in real time. 2 The concentration is controlled by the electromagnetic valve, and the air intake volume is dynamically adjusted to maintain steady state (for example, the IR sensor can restore the set value within 5 minutes after frequent door opening). The humidity control module uses an evaporation plate or an active humidification system (such as a steam generator) to reduce osmotic pressure fluctuations and ensure the stability of cell metabolism.

[0004] In the existing culture box, multiple shelves are used to place culture containers, and the shelves and the box are limited by slots. Since different cell culture scenarios have different compatibility requirements for container height, it is inconvenient to adjust the distance between the shelves and partitions, which reduces the space utilization of the box. Therefore, a new microsphere culture device is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a novel microsphere culture device to solve the problems raised in the above background technology.

[0006] The technical solution adopted by the present invention is: A novel microsphere culture device comprises a box body, a plate body fixed to the inner side wall of the box body, a slide plate slidably connected to the inner side wall of the plate body, and a shelf arranged on one side of the slide plate, wherein the inner side wall of the plate body is provided with a height adjustment component, and the height adjustment component comprises: A second synchronous wheel, symmetrically rotatably connected to the inner wall of the plate body; A first synchronous wheel, rotatably connected to the inner side wall of the plate body, and the first synchronous wheel is used to connect to a driving device; A synchronous belt, the outer side walls of the two synchronous wheels are connected to the synchronous belt for transmission, the synchronous belt is connected to the slide plate through a limit assembly, and the transmission of the synchronous belt drives the slide plate on the limit assembly to move upward or downward.

[0007] Optionally, two plates are provided, which are symmetrically arranged on the inner wall of the box.

[0008] Optionally, the height adjustment components are provided in two groups, which are respectively arranged on the plate body and symmetrically arranged.

[0009] Optionally, a transmission box is provided on the inner side wall of the box body.

[0010] Optionally, the limiting component includes: A box body, sliding on the plate body; A rod body slides on the box body, with one end fixing the slide plate and the other end fixing the pressure plate; The spring is sleeved on the outer side wall of the spring, one end of the spring is fixed on the inner side wall of the box body, and the other end of the spring is fixed on the inner side wall of the pressure plate.

[0011] Optionally, the inner side wall of the pressure plate has a tooth structure that matches the synchronous belt.

[0012] Optionally, a limit plate is symmetrically arranged on one side of the pressure plate close to the interior of the box body, and a groove body matching the synchronous belt is opened on one side of the box body.

[0013] Optionally, an adjustment component is provided on the shelf, and the adjustment component is pressed so that the shelf applies force to the rod body, and at this time, the pressure plate is not in contact with the synchronous belt.

[0014] Optionally, the adjustment component includes: A frame, disposed on the shelf; A shaft body 1 is rotatably connected to the inner wall of the frame, a knob is fixed to one end of the frame, and a bevel gear 1 is fixed to the other end; Axle body 2 is rotatably connected to the inner wall of the frame, and sliders are threaded at both ends. The slider is slidably connected to the frame, and a bevel gear 2 that cooperates with the bevel gear 1 is fixed to the outer wall of the shaft body 2. Compared with the prior art, the beneficial effects of the present invention are: a plurality of shelves are driven upward or downward by the transmission of a synchronous belt, which is convenient for quickly adjusting the distance between the shelf and the box body, and by releasing the restraining effect of the limit assembly and controlling the operation of the synchronous belt again, the position of the partition on the synchronous belt can also be directly manually controlled, which is convenient for quickly adjusting the distance between the shelf and the partition, thereby facilitating the adjustment of the distance between the partition according to the needs of users to place different culture containers. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a schematic diagram of the structure in this application; Figure 2 This is a schematic diagram of the internal structure of the hidden box in this application Figure 1 ; Figure 3 It is a schematic diagram of the structure of the height adjustment component in this application; Figure 4 It is a schematic diagram of the structure of the limit assembly in this application; Figure 5 It is a schematic diagram of the structure of the regulating component in this application; Figure 6 This is a schematic diagram of the internal structure of the hidden box in this application Figure 2 .

[0017] Reference numerals: 100, box body; 110, plate body; 120, shelf; 130, slide plate; 200, height adjustment assembly; 210, synchronous belt; 220, synchronous wheel 1; 230, synchronous wheel 2; 240, transmission box; 300, limit assembly; 310, pressure plate; 311, slot; 320, box body; 330, rod body; 340, spring; 350, limit plate; 400, adjustment assembly; 410, knob; 420, shaft body 1; 430, bevel gear 1; 440, shaft body 2; 450, bevel gear 2; 460, slider; 470, frame; 480, telescopic rod. DETAILED DESCRIPTION

[0018] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the products of the present invention are conventionally placed when used, or are the orientations or positional relationships conventionally understood by those skilled in the art, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0019] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0020] In view of the current existing technology, multiple shelves are used in the existing culture box to place culture containers, and the shelves and the box are limited by slots. Since different cell culture scenarios have different compatibility requirements for container height, it is inconvenient to adjust the distance between the shelves and the partitions, which reduces the space utilization of the box. Therefore, a new microsphere culture device is proposed.

[0021] The present application uses a synchronous belt transmission to drive multiple shelves to move upward or downward, which is convenient for quickly adjusting the distance between the shelves and the box body, and by releasing the work of the limit assembly and controlling the operation of the synchronous belt again, the position of the partition on the synchronous belt can also be directly manually controlled, which is convenient for quickly adjusting the distance between the shelves and the partitions, thereby facilitating the adjustment of the distance between the partitions according to the needs of users to place different culture containers.

[0022] like Figure 1-6As shown, an embodiment of the present invention provides a novel microsphere culture device, a novel microsphere culture device, comprising a box body 100, a plate body 110 fixed to the inner wall of the box body 100, a slide plate 130 slidably connected to the inner wall of the plate body 110, and a shelf 120 arranged on one side of the slide plate 130, the inner wall of the plate body 110 is provided with a height adjustment component 200, the height adjustment component 200 comprises: a synchronous wheel 230, symmetrically connected to the inner wall of the plate body 110 for rotation; a synchronous wheel 1 220, connected to the inner wall of the plate body 110 for rotation, the synchronous wheel 1 220 is used to connect a driving device; a synchronous belt 210, the outer walls of the two synchronous wheels 230 are connected to the synchronous belt 210 for transmission, the synchronous belt 210 is connected to the slide plate 130 through a limit assembly 300, and the transmission of the synchronous belt 210 drives the slide plate 130 on the limit assembly 300 to move upward or downward.

[0023] Specifically, the microsphere culture device product of the present application is a carbon dioxide incubator, which mainly controls the temperature, humidity, and CO 2 The culture device for simulating the microenvironment in vivo by adjusting the concentration and pH value is a prior art and will not be described in detail here; The user needs to use different culture containers during the culture process, so the user needs to adjust the distance between the shelf 120 and the plate body 110. When adjusting, the driving device drives the synchronous wheel 1 220 to rotate, and the rotation of the synchronous wheel 1 220 drives the synchronous belt 210 to drive. At this time, the synchronous belt 210 drives the two synchronous wheels 230 to rotate, and the shelf 120 on the synchronous belt 210 is upward or downward according to the transmission direction of the synchronous belt 210. Refer to the attached Figure 3 , the synchronous belt 210 drives the two shelves 120 to move upward or downward, changing the distance between the shelves 120 and the top surface of the box 100 or changing the distance between the shelves 120 and the bottom surface of the box 100. In this way, only the space of the two areas can be adjusted, so that the user can place the culture container in the adjusted area; When there are too many culture containers or too many types of heights, the distance between the shelves 120 needs to be adjusted. The shelf 120 is pressed manually, and the shelf 120 presses the limit assembly 300. The limit assembly 300 releases the limit between the synchronous belt 210. At this time, the drive device is controlled again, and the other shelf 120 is driven by the synchronous belt 210 to change the distance between the shelf 120, or the user directly moves the shelf 120 up or down on the shelf 120 to change the distance between the two shelves 120. Both methods can adjust the distance between the two shelves 120, and can be selected according to the specific situation of the user. For example, if the adjustment distance is too short, it can be manually adjusted, otherwise it can be electrically driven. The drive device is not reflected in the drawings and belongs to the prior art and is not described here.

[0024] In some embodiments, two plates 110 are provided and symmetrically arranged on the inner wall of the box body 100 .

[0025] Furthermore, the shelves 120 in the present application are not limited to two, and the height adjustment assembly 200 can be hidden by the plate body 110. The plate body 110 and the box body 100 are connected and fixed by bolts. The two height adjustment assemblies 200 can be removed at the same time by removing the two plate bodies 110, thereby facilitating maintenance by users.

[0026] In some embodiments, two groups of height adjustment components 200 are provided, which are respectively disposed on the plate body 110 and are symmetrically arranged; Furthermore, controlling the operation of the two height adjustment components 200 simultaneously can drive the height of the shelf 120 , thereby ensuring that the shelf 120 rises or falls smoothly.

[0027] In some embodiments, a transmission box 240 is disposed on the inner wall of the housing 100 .

[0028] Furthermore, the transmission box 240 is mainly composed of a synchronous belt, a synchronous wheel, and a motor. The motor drives the synchronous wheel to rotate, and the transmission of the synchronous belt drives the other two synchronous wheels to rotate. At this time, the two synchronous wheels drive the two shaft rotation rods, and the rotation of the shaft drives the synchronous wheel 220 to rotate, and then drives the synchronous belt 210 to transmit. The synchronous belt, synchronous wheel, and motor are not reflected in the accompanying drawings. They are existing technologies and will not be repeated here.

[0029] In some embodiments, the limit assembly 300 includes: a box body 320, which slides on the plate body 110; a rod body 330, which slides on the box body 320, with one end fixed to the slide plate 130 and the other end fixed to the pressure plate 310; a spring 340, which is sleeved on the outer wall of the spring 340, with one end fixed to the inner wall of the box body 320 and the other end fixed to the inner wall of the pressure plate 310.

[0030] Furthermore, when the user releases the fixation between the slide plate 130 and the synchronous belt 210, the user applies pressure to the opposite sides of the two slide plates 130 with his hands, so that the slide plate 130 drives the rod body 330 to move on the box body 320 and drives the pressure plate 310 to move to one side. At this time, the pressure plate 310 and the synchronous belt 210 are no longer in contact. When applying pressure to the slide plate 130, the user can remove the shelf 120 from the slide plate 130 and operate it. After no longer in contact, the box body 320 can be directly slid to adjust the height of the box body 320 on the synchronous belt 210, thereby facilitating the adjustment of the distance between multiple shelves 120. After the slide plate 130 is no longer pressed, the elastic reset of the spring 340 can move the pressure plate 310 to the initial position.

[0031] In some embodiments, the inner wall of the pressure plate 310 has a tooth structure that matches the tooth structure on the synchronous belt 210 .

[0032] Furthermore, the pressing plate 310 is restricted on the synchronous belt 210 by meshing the teeth on the pressing plate 310 with the teeth on the synchronous belt 210 , wherein the teeth are not shown in the drawings.

[0033] In some embodiments, a limit plate 350 is symmetrically disposed on one side of the pressure plate 310 close to the interior of the box body 320 , and a groove body that cooperates with the synchronous belt 210 is opened on one side of the box body 320 .

[0034] Furthermore, the groove body is a clamping groove 311 for limiting the sliding position of the synchronous belt 210 . When the pressing plate 310 moves, the limiting plate 350 also moves accordingly to ensure the position between the pressing plate 310 and the synchronous belt 210 .

[0035] In some embodiments, an adjustment assembly 400 is disposed on the shelf 120 , and the shelf 120 applies force to the rod body 330 by pressing the adjustment assembly 400 , and at this time, the pressure plate 310 is not in contact with the synchronous belt 210 .

[0036] Frame 470 is arranged on shelf 120; shaft body 1 420 is rotatably connected to the inner wall of frame 470, a knob 410 is fixed to one end of frame 470, and a bevel gear 1 430 is fixed to the other end; shaft body 2 440 is rotatably connected to the inner wall of frame 470, both ends of which are threadedly connected with sliders 460, slider 460 is slidably connected to frame 470, and a bevel gear 2 450 matching bevel gear 1 430 is fixed to the outer wall of shaft body 2 440.

[0037] Furthermore, when the shelf 120 is engaged with the slide plate 130 and the user needs to adjust the distance between the shelf 120, the user first rotates the knob 410, and the knob 410 drives the shaft body 1 420 to rotate, and the shaft body 1 420 drives the bevel gear 1 430 to rotate, and the bevel gear 1 430 engages with the bevel gear 2 450. The rotation of the bevel gear 2 450 drives the shaft body 2 440 to rotate, and the shaft body 2 440 drives the sliders 460 at both ends to move in opposite directions, and then the slider 460 squeezes the slide plate 130, thereby releasing the limit fixation between the pressure plate 310 and the synchronous belt 210. During the squeezing process, the groove body of the slide plate 130 will not separate from the two sides of the shelf 120.

[0038] Furthermore, a telescopic rod 480 is provided between the two frames 470 to connect the two shelves 120 , so that the user can collectively pull out the multiple shelves 120 .

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A novel microsphere culture device, characterized in that: The invention comprises a box body (100), a plate body (110) fixed to the inner side wall of the box body (100), a slide plate (130) slidably connected to the inner side wall of the plate body (110), and a shelf (120) arranged on one side of the slide plate (130), wherein the inner side wall of the plate body (110) is provided with a height adjustment component (200), and the height adjustment component (200) comprises: A second synchronous wheel (230) is symmetrically rotatably connected to the inner wall of the plate body (110); A synchronous wheel (220) is rotatably connected to the inner wall of the plate body (110), and the synchronous wheel (220) is used to connect to a driving device; A synchronous belt (210), the outer side walls of the two synchronous wheels (230) are connected to the synchronous belt (210) in a transmission manner, the synchronous belt (210) and the slide plate (130) are connected via a limit assembly (300), and the transmission of the synchronous belt (210) drives the slide plate (130) on the limit assembly (300) to move upward or downward.

2. A novel microsphere culture device according to claim 1, characterized in that: Two plates (110) are provided, and are symmetrically arranged on the inner side wall of the box body (100).

3. A novel microsphere culture device according to claim 2, characterized in that: The height adjustment components (200) are provided in two groups, which are respectively arranged on the plate body (110) and are symmetrically arranged.

4. A novel microsphere culture device according to claim 3, characterized in that: A transmission box (240) is provided on the inner side wall of the box body (100).

5. A novel microsphere culture device according to claim 2, characterized in that: The limiting assembly (300) comprises: A box body (320) slides on the plate body (110); A rod body (330) slides on the box body (320), with one end fixing the slide plate (130) and the other end fixing the pressure plate (310); The spring (340) is sleeved on the outer wall of the spring (340), one end of which is fixed to the inner wall of the box body (320), and the other end of which is fixed to the inner wall of the pressing plate (310).

6. A novel microsphere culture device according to claim 5, characterized in that: The inner side wall of the pressure plate (310) has a tooth structure that matches the tooth structure on the synchronous belt (210).

7. A novel microsphere culture device according to claim 6, characterized in that: A limiting plate (350) is symmetrically arranged on one side of the pressure plate (310) close to the interior of the box body (320), and a groove body matching the synchronous belt (210) is opened on one side of the box body (320).

8. A novel microsphere culture device according to claim 7, characterized in that: The shelf (120) is provided with an adjustment component (400), and pressing the adjustment component (400) causes the shelf (120) to apply force to the rod body (330), and at this time, the pressure plate (310) is not in contact with the synchronous belt (210).

9. A novel microsphere culture device according to claim 8, characterized in that: The adjustment component (400) comprises: A frame (470) is disposed on the shelf (120); A shaft body (420) is rotatably connected to the inner wall of the frame (470); a knob (410) is fixed to one end of the frame (470) and a bevel gear (430) is fixed to the other end; Axle body 2 (440) is rotatably connected to the inner wall of the frame (470), and sliders (460) are threadedly connected at both ends. The sliders (460) are slidably connected to the frame (470), and a bevel gear 2 (450) that matches the bevel gear 1 (430) is fixed to the outer wall of the shaft body 2 (440).

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