Pollen collector and pollen concentration determination method

Abstract

The invention discloses a pollen collector and a pollen concentration determination method.The pollen collector comprises a supporting frame and a collecting assembly installed on the supporting frame and used for collecting pollen, the collecting assembly is composed of a rotating speed regulator, a rotating arm and collecting rods, the collecting rods are vertically arranged at the two top ends of the rotating arm, the width of the cross section of each collecting rod is 4-6 mm, and the length of each collecting rod is 25-30 mm; in the pollen collecting process, rotation is conducted with the small air displacement, disturbance to surrounding air is reduced to the maximum degree, and pollen can be collected more efficiently and accurately. The method comprises the following steps: dyeing a collection rod full of pollen, placing the collection rod under a microscope, optionally selecting several different areas, determining the amount of pollen in each area, and calculating the concentration of pollen at a measured position by combining the volume of air discharged in the collection process, so that the method omits a treatment step of filtering impurities, avoids taking out part of pollen during filtering, and is more accurate in concentration measurement.

Description

Technical Field

[0001] The invention relates to the field of pollen collection, and in particular to a pollen collector and a pollen concentration measuring method. Background Art

[0002] Accurately collecting and quantifying the concentration of pollen in the air can provide important information for studying disciplines such as plant pollination biology, environmental ecology, and pollen pathogen transmission. There are various methods for collecting pollen, which can be divided into natural sedimentation method and active collection method according to the collection method. The natural sedimentation method is easily affected by climatic factors and impurities such as dust, and it is difficult to truly reflect the accurate pollen concentration in the air at the collection point. The active collection method uses the air sampler on the collector to extract a certain volume of air, and the pollen in the air is intercepted by the collection medium in the sampler. However, this method causes a large disturbance to the surrounding air when extracting air, which reduces the pollen collection efficiency and accuracy, and requires professional air sampling equipment, which is costly and relatively complex to operate. Summary of the invention

[0003] Purpose of the invention: The purpose of the present invention is to provide a pollen collector that reduces air disturbance during the collection process and thus improves the collection accuracy. Another purpose of the present invention is to provide a method for measuring pollen concentration based on the above pollen collector and with more accurate concentration measurement.

[0004] Technical solution: The pollen collector described in the present invention includes a support frame and a collecting assembly installed on the support frame for collecting pollen. The collecting assembly includes a speed regulator, a rotating arm whose rotation speed is adjusted by the speed regulator, and a collecting rod vertically arranged on the rotating arm.

[0005] Preferably, the collecting rods are arranged at the left and right top ends of the rotating arm. The symmetrical arrangement can make the collecting assembly more stable during the rotation process and reduce the disturbance to the air. The cross-sectional width of the collecting rod is 4 to 6 mm, and the length of the collecting rod is 25 to 30 mm, which can not only meet the needs of collecting pollen, but also avoid the disturbance of the air caused by excessive contact surface with the air during rotation, thereby affecting the accuracy of subsequent pollen concentration measurement.

[0006] Furthermore, the installation height on the support frame can be adjusted according to the need to measure the pollen concentration in the air at different heights. The position of the collecting component on the support frame can be changed by installing the claws, so that pollen at different heights can be collected at the same time, and the pollen concentration at different heights can be accurately measured.

[0007] Preferably, the speed regulator can also set different speeds according to different types of pollen to make the collection more accurate.

[0008] The method for measuring pollen concentration using a pollen collector of the present invention comprises the following steps:

[0009] (a) Adjust the height of the collecting assembly, set the speed of the speed regulator, and the pollen collector starts working;

[0010] (b) Remove the pollen-stained collecting rod and dye the pollen on the collecting rod. The effective collecting area of ​​a single collecting rod is recorded as S r ;

[0011] (c) Place the collecting rod under the microscope, randomly select several different areas and determine the number of pollen in each area. The total area of ​​the selected areas is S 0 The average pollen number in the selected area is recorded as N m , the number of pollen on the collecting stick N p =N m ×S r / S 0 , the volume of air displaced by the collecting rod at the set rotation speed is V, and the measured pollen concentration is N p / V.

[0012] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages: (1) the disturbance to the surrounding air is minimized during the pollen collection process, and pollen in the air is collected more efficiently and accurately; (2) the processing step of filtering impurities is eliminated, avoiding the removal of part of the pollen when filtering impurities, and the concentration measurement is more accurate; (3) the vertical arrangement of the collecting rod can reduce the deposition of dust and impurities, and reduce the impact of impurities on the measurement results; (4) pollen at different heights can be collected at the same time, and the pollen concentration at different heights can be measured, which is low in cost and simple to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural schematic diagram of the present invention,

[0014] Figure 2 It is a schematic diagram of the structure of the collection component in the present invention. DETAILED DESCRIPTION

[0015] The technical solution of the present invention is further described below in conjunction with the accompanying drawings.

[0016] As shown in the figure, a pollen collector described in the present invention includes a support frame 1, a speed regulator 2, a rotating arm 3, a collecting rod 4, a mounting claw 5 and a mobile power supply 6. The support frame 1 is made of metal, and its bottom is in the shape of a triangular bracket. The upper part of the support frame is a vertical rod that can be retracted. When in use, the vertical rod is extended, and when folded, the vertical rod is retracted for easy carrying. Multiple collection components can be fixed on the support frame, and pollen of different heights can be collected at the same time, which can ensure full contact with the air to capture pollen in the air.

[0017] The collecting assembly is horizontally mounted on the vertical rod through the mounting claw 5. The end of the collecting assembly extending outward is provided with a speed regulator 2. The mobile power supply 6 is used to power the speed regulator. The speed regulator is provided with a switch and a knob for adjusting the speed. The speed regulator is adjusted to a suitable speed according to different plant pollens, preferably 600-1000rpm. The shell of the speed regulator is preferably made of PVC material, which has the functions of protecting internal components, waterproofing, dustproofing and sun protection, etc. Other materials with the above functions can also be used to ensure that it can work normally in different outdoor environments. The rotating arm 3 is horizontally arranged at the top of the speed regulator and connected to the top center output end of the speed regulator. The rotating arm can rotate according to the speed set by the speed regulator. The left and right top ends of the rotating arm are opened, and the collecting rod 4 is vertically inserted into the holes. The diameter of the collecting rod is 4-6 mm, and the length of the collecting rod is 25-30 mm. Experimental data show that the pollen collection efficiency is the highest when the collecting rod is within this size range. When the length of the collecting rod exceeds 30 mm, the pollen collection efficiency decreases as the length increases, indicating that the larger contact area with the air causes greater disturbance to the air, so the collection efficiency is low; when the collecting rod is 25-30 mm long, the collecting rod with a cross-sectional width greater than 6 mm or less than 4 mm has a lower pollen collection efficiency than the collecting rod with a cross-sectional width of 4-6 mm. The collecting rod with this diameter size can reduce the disturbance to the surrounding air as much as possible during the collection process, reduce the impact of air disturbance on the collection of pollen, and collect pollen in the air more efficiently and accurately. Holes can also be opened in other parts of the rotating arm, but the positions of the holes must be symmetrical to ensure that the collecting rod is symmetrical on the left and right, avoiding increased shaking caused by asymmetry during rotation and disturbing the air, which affects the accuracy of pollen collection.

[0018] Using the pollen collector, a pollen concentration determination method according to the present invention comprises the following steps:

[0019] (a) According to the need to measure the pollen concentration in the air at different heights, determine the height of the collecting assembly, and connect the collecting assembly to the vertical rod with the mounting claws; set the speed of the speed regulator according to the type of pollen to be measured; evenly apply vaseline or animal fat on the windward side of the collecting rod, insert it into the hole of the rotating arm, turn on the mobile power supply, rotate the rotating arm, and adjust the speed of the collecting rod by the speed regulator; replace the collecting rod every hour, put the collecting rod stained with pollen in a dustproof storage box, and then put a new collecting rod to continue collecting pollen until the collection is completed;

[0020] (b) The pollen on the collecting rod was stained with 1% acetic acid magenta for 15 minutes to remove dust and other interferences. The stained pollen was quantified under a microscope. The effective collection area of ​​a single collecting rod was recorded as S. r ;

[0021] (c) Place the stained collecting rod under a microscope, randomly select several different areas and determine the number of pollen in each area. The total area of ​​the selected areas is S 0 The average pollen number in the selected area is recorded as N m , the number of pollen on the collecting stick N p =N m ×S r / S 0 , the volume of air displaced by the collecting rod at the set rotation speed is V, and the measured pollen concentration is N p / V.

Claims

1. A pollen collector, comprising a support frame (1) and a collecting assembly mounted on the support frame for collecting pollen, characterized in that: The collecting assembly comprises a rotation speed regulator (2), a rotating arm (3) whose rotation speed is regulated by the rotation speed regulator, and a collecting rod (4) vertically arranged on the rotating arm.

2. The pollen collector according to claim 1, characterized in that: The cross-sectional width of the collecting rod (4) is 4 to 6 mm, and the length of the collecting rod is 25 to 30 mm.

3. The pollen collector according to claim 1, characterized in that: The collecting rods (4) are vertically and symmetrically arranged at the left and right top ends of the rotating arm.

4. The pollen collector according to claim 1, characterized in that: The height of the collecting assembly on the supporting frame is adjustable.

5. The pollen collector according to claim 1, characterized in that: The collecting assembly also includes a mounting claw (5), through which the position of the collecting assembly on the supporting frame can be changed.

6. The pollen collector according to claim 1, characterized in that: The rotation speed regulator (2) can be set to different rotation speeds according to the type of pollen.

7. A method for measuring pollen concentration using the pollen collector according to claim 1, characterized in that: The following steps are involved: (a) Adjust the height of the collecting assembly, set the speed of the speed regulator, and the pollen collector starts working; (b) Remove the pollen-stained collecting rod and dye the pollen on the collecting rod. The effective collecting area of ​​a single collecting rod is recorded as S r ; (c) Place the collecting rod under the microscope, randomly select several different areas and determine the number of pollen in each area. The total area of ​​the selected areas is S0, and the average pollen number of the selected areas is N. m , the number of pollen on the collecting stick N p =N m ×S r / S0, the volume of air displaced by the collecting rod at the set rotation speed is V, and the measured pollen concentration is N p / V.

8. A method for measuring pollen concentration according to claim 7, characterized in that: The height of the collecting assembly in step a is adjustable within a range of 0.5 m to 10 m.

9. A method for measuring pollen concentration according to claim 7, characterized in that: The speed of the speed regulator in step a is adjustable within the range of 600 to 1000 rpm.

10. A method for measuring pollen concentration according to claim 7, characterized in that: In the step b, acetic acid magenta is used to dye the pollen.

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