High-viscosity liquid pipetting device and method based on corona discharge

Abstract

The invention belongs to the technical field of microfluidics, and particularly relates to a corona discharge-based high-viscosity liquid pipetting device and method.The corona discharge-based high-viscosity liquid pipetting device comprises a central needle point electrode connected with a negative electrode and used for being in contact with liquid and sucking or releasing the liquid; the plurality of surrounding needle point electrodes are connected with the positive electrode, are distributed around the central needle point electrode, keep a consistent distance with the central needle point electrode, and are used for generating corona discharge and forming ionic wind to drive liquid to move; the micro high-voltage power supply provides electric field energy for corona discharge; and the potentiometer is used for adjusting the output voltage of the micro high-voltage power supply and indirectly controlling the suction amount of the liquid. The electric field force is used for driving the liquid to move, efficient transfer of the high-viscosity liquid can be achieved without a mechanical pump or an additional complex device, accurate control over the liquid volume can be achieved by adjusting the voltage, the operation efficiency is greatly improved, and the application range is greatly widened.

Description

Technical Field

[0001] The present invention belongs to the technical field of microfluidics, and particularly relates to a high-viscosity liquid pipetting device and method based on corona discharge. Background Art

[0002] Liquid transfer is a common operation in fields such as chemistry, biology, and industry, including the transfer of high-viscosity liquids. With the increasing demand for high-precision fluid operations in various fields, the following requirements are put forward for high-viscosity liquid transfer technology: Wide applicable liquid viscosity range: It is necessary to be able to handle liquids with various viscosity ranges to meet the needs of different fields. High-precision liquid volume control: Especially in fields such as microfluidic systems, nanomanufacturing, and drug research and development, it is necessary to accurately control the volume of the transferred liquid. Universality and efficiency: When using operating equipment to transfer liquids, there are no strict conditions required for the liquids. For example, it is not required that the liquid has conductivity, and the overall equipment should have compactness and portability.

[0003] However, for the pipetting of high-viscosity liquids, the existing technologies face huge challenges. Traditional pipettes (such as mechanical or electronic pipettes) and fluid handling systems are usually based on the principles of mechanical liquid suction, pressure-driven, or gravity flow. However, these methods show obvious limitations when dealing with liquids with relatively high viscosities (such as silicone oil, resin, lubricant, etc.). The specific manifestations are as follows:

[0004] Efficiency problem: Mechanical pipettes need to drive air pressure to suck liquid through a piston. The slow flow characteristics of high-viscosity liquids make it difficult to quickly complete the liquid suction and discharge operations, seriously reducing the working efficiency.

[0005] Liquid residue: High-viscosity liquids are prone to forming residues inside the pipette, resulting in contamination and measurement errors, which are unacceptable in precision experiments or scenarios with high purity requirements.

[0006] Low volume control accuracy: When mechanical pipettes handle high-viscosity liquids, the surface tension and adhesion of the liquid make it difficult to accurately control the suction volume. Especially when transferring trace amounts of liquid, the error is more significant.

[0007] Technical bottlenecks in the treatment of special liquids: Non-conductive liquids (such as silicone oil) are difficult to drive the flow through conventional electrical methods (such as electrostatic fields or electrolysis) due to the lack of free ions. For ultra-high viscosity liquids (such as viscosity greater than 10000 cSt), existing pump systems need to increase additional pressure or temperature to reduce the liquid viscosity, which not only increases the operation complexity but also may change the liquid properties. The existing microfluidic systems and automated liquid handling equipment have poor applicability in the operation of high-viscosity liquids and mainly focus on the treatment of low to medium viscosity liquids.

[0008] Facing the above challenges, researchers have tried to improve the transfer technology of high-viscosity liquids in the following ways: (1) Heating-assisted technology: By heating, the viscosity of the liquid is reduced, making it easier to flow. However, heating will change the chemical properties of the liquid and is not applicable especially in sensitive liquids (such as biological samples or special chemicals). (2) Pressure-assisted driving: Pneumatic or hydraulic devices are used to push the liquid to flow, but the pressure system is complex and it is difficult to control trace amounts of liquid, and the equipment cost is relatively high. (3) Electrical driving technology: Electro-wetting technology and electric field manipulation technology developed in recent years show potential in the operation of trace amounts of liquid, but they have poor adaptability to high-viscosity liquids, require the liquid to have conductivity, and have strict requirements for the conductivity of the liquid.

[0009] Therefore, how to achieve efficient and precise pipetting of the above liquids through a simple and portable device is a problem that needs to be overcome. Summary of the Invention

[0010] To solve the above technical problems, the present invention provides a high-viscosity liquid pipetting device and method based on corona discharge. The pipetting device provided by the present invention uses the electric field force to drive the liquid to move. Without a mechanical pump or additional complex devices, it can achieve efficient transfer of high-viscosity (viscosity can be up to 30,000 cSt) and non-conductive liquids, and the precise control of the liquid volume can be achieved by adjusting the voltage, greatly improving the operation efficiency and application range. The device of the present invention is simple, portable, precise, and has strong universality, filling the gap in the existing technology and being able to meet the needs of high-viscosity liquid transfer.

[0011] The present invention is specifically realized through the following technical solutions.

[0012] The present invention provides a high-viscosity liquid pipetting device based on corona discharge, which includes a housing. One end of a central needle tip electrode is installed inside the housing, and a plurality of peripheral needle tip electrodes are installed around the central needle tip electrode. The other ends of the central needle tip electrode and the peripheral needle tip electrodes both extend to the outside of the housing; a micro high-voltage power supply is installed inside the housing. The central needle tip electrode is electrically connected to the negative pole of the micro high-voltage power supply and is used to contact the liquid and suck or release the liquid; a plurality of peripheral needle tip electrodes are electrically connected to the positive pole of the micro high-voltage power supply and are used to generate corona discharge and form an ionic wind to drive the liquid to move; a potentiometer is installed on the housing, and the potentiometer is electrically connected to the micro high-voltage power supply and is used to adjust the output voltage of the micro high-voltage power supply to indirectly control the suction volume of the liquid.

[0013] Preferably, the liquid is a liquid with a viscosity of 50 cSt to 30,000 cSt and is a non-conductive liquid.

[0014] Preferably, a plurality of peripheral needle tip electrodes are symmetrically distributed around the central needle tip electrode and maintain a consistent spacing from the central needle tip electrode.

[0015] Preferably, the diameter of the central needle tip electrode is 100 µm, and the distance between the surrounding needle tip electrodes and the central needle tip electrode is 3 mm to 15 mm, preferably 8 mm.

[0016] Preferably, the diameter of the central needle tip electrode is smaller than that of the surrounding needle tip electrodes, and the diameter range of the surrounding needle tip electrodes is 150 µm to 450 µm.

[0017] Preferably, an installation part is arranged inside the housing. One end of the central needle tip electrode is installed in the middle of the installation part, and one ends of several surrounding needle tip electrodes are installed on the installation part and are circumferentially distributed around the central needle tip electrode. A notch is formed at one end of the housing, and the other ends of the central needle tip electrode and several surrounding needle tip electrodes extend to the outside of the housing through the notch.

[0018] Preferably, a second switch is further included. The second switch is installed on the outside of the housing and is electrically connected to the central needle tip electrode and the negative electrode of the micro high-voltage power supply, and is used to control the connection between the central needle tip electrode and the negative electrode, thereby determining whether to suck or release the liquid.

[0019] Preferably, a first switch is further included. The first switch is installed on the outside of the housing. The first switch is electrically connected to the positive electrode of the micro high-voltage power supply and the surrounding needle tip electrodes, and is used to control the on-off between the surrounding needle tip electrodes and the micro high-voltage power supply. The first switch serves as the main switch for whether the micro high-voltage power supply discharges.

[0020] A display screen is installed on the outside of the housing and is used to display the current voltage value in real time.

[0021] A battery is electrically connected to the micro high-voltage power supply, and the display screen is electrically connected to the battery. The battery is preferably a lithium battery. The display screen is used to display the current voltage value in real time, specifically an LED voltage display screen.

[0022] Preferably, an end cover is detachably connected to the end of the housing away from the central needle tip electrode, and the second switch and the first switch are installed on the end cover.

[0023] The present invention also provides a method for pipetting high-viscosity liquid based on corona discharge, including the following steps: Connect the surrounding needle tip electrodes to the positive electrode of the micro high-voltage power supply, and set a suitable voltage of the micro high-voltage power supply by adjusting the potentiometer.

[0024] Vertically insert the central needle tip electrode into the liquid surface, connect the central needle tip electrode to the negative electrode of the micro high-voltage power supply. After the high-voltage electric field is formed, ion wind is generated through corona discharge to drive the liquid to rise along the central needle tip electrode, and the liquid is sucked.

[0025] After the droplet reaches a stable state, the central needle electrode is removed from the liquid surface and moved above the target container. The central needle electrode is disconnected from the negative electrode of the micro high-voltage power supply, so that the sucked liquid is released from the central needle electrode into the target container under the action of gravity and residual electric field force.

[0026] During the liquid sucking process, when the liquid viscosity is relatively high, after the liquid sucking is saturated, first disconnect the surrounding needle electrodes from the positive electrode of the micro high-voltage power supply, and then remove the central needle electrode from the liquid surface to avoid the tip from sucking the dielectric liquid during the upward movement of the central needle electrode away from the liquid surface. Then reconnect the surrounding needle electrodes to the positive electrode of the micro high-voltage power supply to keep the droplet stationary in place, and then transfer it above the target container.

[0027] Compared with the prior art, the present invention has the following beneficial effects: The high-viscosity liquid pipetting device of the present invention based on corona discharge significantly improves the efficiency, accuracy and applicability of high-viscosity liquid transfer, and brings the following obvious technical effects compared with the prior art: The device of the present invention is provided with a central needle electrode, and several surrounding needle electrodes are installed around the central needle electrode. The central needle electrode is electrically connected to the negative electrode of the micro high-voltage power supply and is used to contact the liquid and suck or release the liquid; several surrounding needle electrodes are electrically connected to the positive electrode of the micro high-voltage power supply and are used to generate corona discharge and form an ion wind to drive the liquid to move; the potentiometer is electrically connected to the micro high-voltage power supply and is used to adjust the output voltage of the micro high-voltage power supply to indirectly control the liquid sucking amount. The above-mentioned central needle electrode, surrounding needle electrodes, micro high-voltage power supply and potentiometer are integrated in the shell, and the structure is simple and portable.

[0028] The above device forms an ionic wind through corona discharge to drive the liquid flow, and can efficiently complete the liquid suction and drainage operations without a mechanical pump or heating assistance. Experiments have proved that the present invention can complete the suction and transfer of non-conductive liquids with a wide range of viscosities (50 cSt to 30,000 cSt), greatly improving the efficiency of high-viscosity liquid transfer. By adjusting the power supply voltage and electric field strength, the present invention can accurately control the liquid suction volume, and the operation accuracy can reach the nanoliter level, which is suitable for high-precision microfluidic experiments and liquid handling. In laboratory operations, especially in chemical experiments, drug research and development, and microfluidic systems with strict requirements for liquid volume, it provides extremely high volume control reliability. The device of the present invention is applicable to the treatment of high-viscosity non-conductive liquids, breaking through the limitations of traditional pipetting devices on liquid conductivity and low viscosity, and significantly expanding the application scenarios of liquid handling. Moreover, the present invention simplifies the equipment structure and reduces the operation complexity and cost: the present invention adopts a miniaturized integrated high-voltage power supply, with a simple structure, without complex mechanical devices, pressure systems or heating assistance devices, reducing the manufacturing cost of the equipment. The equipment is easy to operate, can be designed in a portable and compact manner, and is easy to promote in laboratory and industrial environments.

[0029] In addition, the present invention also has the following advantages: Avoid liquid residue and ensure the integrity of transfer: The present invention drives the liquid movement through electric field force. Compared with the traditional mechanical liquid suction method, it avoids the situation of liquid remaining inside the liquid suction device. It ensures the cleanliness and integrity of liquid transfer, reduces the risk of liquid waste and pollution, and is especially suitable for scenarios with high requirements for liquid purity.

[0030] Provide the possibility of automated and efficient operation: The design of the present invention supports integration with an automated platform and can be applied to automated workflows such as high-throughput screening and microfluidic chips to achieve unmanned operation of liquid transfer. While maintaining high efficiency and precision, the device can stably operate multiple cycle operations to meet the efficiency requirements of modern laboratories and industries.

[0031] Environmental protection and safety: The present invention drives liquid transfer through the action of an electric field, without involving auxiliary operations such as chemical reagents or mechanical heating, which is environmentally friendly and safe. The design of the high-voltage power supply ensures the safety of the equipment during operation and will not cause damage to the operator or liquid sample.

[0032] Broad application prospects: The technology of the present invention can be widely applied to (1) chemical experiments: the transfer and treatment of high-viscosity reaction liquids; (2) microfluidic systems: high-precision liquid operation and analysis; (3) biotechnology: the treatment of high-viscosity biological samples (such as gels, oils, etc.); (4) industrial manufacturing: the efficient transfer of special liquids such as lubricating oils, resins, and silicone oils. Especially in fields with high requirements for high-viscosity liquid operation (such as cosmetics R & D, pharmaceutical industry, etc.), the present invention has great commercial value.

[0033] The present invention effectively overcomes many problems in the treatment of high-viscosity liquids through a unique corona discharge driving technology, and has the characteristics of high efficiency, applicability, precision and economy. Its technical effects are remarkable. It not only solves the pain points in actual operation, but also shows great market potential in laboratory and industrial applications. Brief Description of the Drawings

[0034] Figure 1 It is an overall view of the high-viscosity liquid pipetting device based on corona discharge of the present invention.

[0035] Figure 2 It is a sectional view of the high-viscosity liquid pipetting device based on corona discharge of the present invention.

[0036] Figure 3 It is an exploded view of the high-viscosity liquid pipetting device based on corona discharge of the present invention.

[0037] Figure 4 It is a diagram of the mechanism of driving liquid movement by corona discharge. Among them, a is a schematic diagram of the formation of ionic wind by corona discharge, and b is a schematic diagram of the action of electric pressure on the liquid.

[0038] Figure 5 Among them, a is a simulation diagram of the electric field distribution between the needle tips, and b is a simulation diagram of the charge distribution on the liquid surface.

[0039] Figure 6 It is a diagram of the liquid suction process.

[0040] Figure 7 It is an effect diagram of liquid suction under different voltage conditions.

[0041] Figure 8 It is an effect diagram of liquid suction under different viscosity conditions.

[0042] Figure 9 It is a diagram of the influence of the number of peripheral needle tip electrodes around the central needle tip electrode on the liquid suction effect.

[0043] Figure 10 Among them, a is the liquid transfer process of a traditional pipette gun, and b~e are the liquid transfer processes using the device of the present invention. Among them, b is the liquid suction, c is the liquid transfer, d is the liquid discharge, and e is the complete liquid discharge.

[0044] Figure 11 Among them, a is a schematic diagram of the principle of sucking liquid using the device of the present invention, and b is a schematic diagram of the principle of discharging liquid using the device of the present invention.

[0045] Description of the Reference Numerals: 1. Central tip electrode; 2. Peripheral tip electrode; 3. Display screen; 4. Battery; 5. Miniature high-voltage power supply; 6. Potentiometer; 7. First switch; 8. Housing; 9. End cap; 10. Second switch. Detailed implementation mode

[0046] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention will be further described below in conjunction with specific embodiments and drawings, but the specific embodiments cited shall not be construed as limiting the present invention. The experimental methods and detection methods described in the following embodiments are all conventional methods unless otherwise specified; the reagents and materials are all commercially available unless otherwise specified.

[0047] The present invention provides a high-viscosity liquid pipetting device based on corona discharge, which includes a housing 8. One end of a central tip electrode 1 is installed inside the housing 8. A plurality of peripheral tip electrodes 2 are installed around the central tip electrode 1. The other ends of the central tip electrode 1 and the peripheral tip electrodes 2 both extend to the outside of the housing 8. A miniature high-voltage power supply 5 is installed inside the housing 8. The central tip electrode 1 is electrically connected to the negative electrode of the miniature high-voltage power supply 5 and is used to contact the liquid and suck or release the liquid. A plurality of peripheral tip electrodes 2 are electrically connected to the positive electrode of the miniature high-voltage power supply 5 and are used to generate corona discharge and form an ionic wind to drive the liquid to move. A potentiometer 6 is installed on the housing 8, and the potentiometer 6 is electrically connected to the miniature high-voltage power supply 5 and is used to adjust the output voltage of the miniature high-voltage power supply 5.

[0048] Based on the innovative mechanism of corona discharge, the present invention proposes a new high-viscosity liquid pipetting device in view of the deficiencies of the prior art. It adopts a structural design of multiple tip electrodes, forms an ionic wind through corona discharge, and realizes the precise sucking and releasing of high-viscosity liquids. Innovatively combines the balance of electric field force and gravity, and can effectively suck high-viscosity liquids to a set height without a mechanical pump or additional pressure. It is suitable for the treatment of high-viscosity non-conductive liquids, filling the gap in traditional electrical driving technologies.

[0049] In the device design, an integrated high-voltage power supply is used to provide a voltage in the range of 0 - 8 kV, realizing the efficient treatment of high-range viscosity liquids (50 cSt to 30000 cSt). The current is only (~μA), and the power is extremely low (~mW). By adjusting the voltage, the liquid suction volume can be flexibly controlled to meet the needs of various scenarios.

[0050] The device has a simple structure and is easy to operate, and is suitable for laboratory and industrial environments. It can transfer high-viscosity liquids efficiently and safely, and has high economic benefits and application prospects.

[0051] In summary, the present invention not only solves the technical bottlenecks existing in the prior art, but also provides a brand-new solution for the pipetting operation of high-viscosity liquids, with remarkable innovation and practicality in technology.

[0052] More specifically, the present invention provides a high-viscosity liquid pipetting device based on corona discharge, as Figures 1 to 3 shown, including: Central tip electrode 1: Connected to the negative pole of the micro high-voltage power supply 5, used to contact the liquid and suck or release the liquid, designed with a fine tip to enhance the electric field strength and reduce the adhesion force of the liquid droplets.

[0053] Peripheral tip electrode 2: Connected to the positive pole of the micro high-voltage power supply 5, maintaining a consistent spacing with the central tip electrode 1, used to generate corona discharge and form an ionic wind to drive the liquid movement. The diameter of the central tip electrode 1 is 100 µm, the diameter of the peripheral tip electrode 2 is 150 µm - 450 µm, and the spacing between the peripheral tip electrode 2 and the central tip electrode 1 is 3 mm - 15 mm.

[0054] Display screen 3: Used to display the current voltage value in real time, facilitating the adjustment and monitoring of working parameters. Specifically, it is an LED voltage display screen.

[0055] Battery 4: Provides the power required for the operation of the device, which is the key to the portability and miniaturization of the device. Preferably a lithium battery.

[0056] Micro high-voltage power supply 5: Generates the required high voltage (0 - 8 kV), with a maximum current of 1 mA, providing electric field energy for corona discharge.

[0057] Potentiometer 6: Used to adjust the output voltage of the micro high-voltage power supply 5, indirectly controlling the liquid suction volume.

[0058] First switch 7: Electrically connected to the positive pole of the micro high-voltage power supply 5 and the peripheral tip electrode 2 respectively, used to control the on-off between the peripheral tip electrode 2 and the micro high-voltage power supply 5, serving as the main switch for whether the micro high-voltage power supply 5 discharges.

[0059] Housing 8: Provides overall protection for the device, ensuring the structural strength and operation safety of the device.

[0060] End cap 9: Used to fix the internal components and facilitate the maintenance, disassembly and assembly of the device. The second switch 10 and the first switch 7 are installed on the end cap 9.

[0061] Second switch 10: Controls the connection between the central tip electrode 1 and the negative pole of the micro high-voltage power supply 5, thereby determining whether to suck or release the liquid.

[0062] Working principle and operation steps of the device of the present invention: Working principle: Liquid suction: Connect the central tip electrode 1 to the negative electrode and the surrounding tip electrodes 2 to the positive electrode. After the high-voltage electric field is formed, ion wind is generated through corona discharge to drive the liquid to rise along the central tip electrode 1. The electric field force causes the liquid to be adsorbed at the middle position of the central tip electrode 1 and keep horizontal with the tips of the surrounding electrodes. The output voltage of the micro high-voltage power supply 5 is adjusted through the potentiometer 6, and then the required liquid volume for suction is adjusted.

[0063] Liquid release: Disconnect the connection between the central tip electrode 1 and the negative electrode, so that the sucked liquid is released from the tip of the central tip electrode 1 to the target container under the action of gravity and residual electric field force.

[0064] Operation steps: Step 1. Preparation work: (1) Turn on the first switch 7 to start the power supply system.

[0065] (2) Adjust the potentiometer 6 to set the appropriate voltage of the micro high-voltage power supply 5.

[0066] Step 2. Liquid suction: (1) Vertically insert the central tip electrode 1 into the liquid surface.

[0067] (2) Turn on the second switch 10 to start liquid suction.

[0068] (3) After the liquid droplet reaches a stable state, turn off the first switch 7 and take out the central tip electrode 1 from the liquid surface to avoid the tip of the central tip electrode 1 sucking the dielectric liquid during the rising process after leaving the liquid surface, and complete the liquid suction. After the extraction is completed, the first switch 7 can be turned on again to keep the dielectric liquid droplet in place. It should be noted that when the viscosity of the liquid is low, after the suction is completed, there is no need to turn off the first switch 7 to prevent the low-viscosity liquid droplet from sliding down due to external forces such as gravity under the action of no electric field force. Just turn off the second switch 10 during the liquid droplet release process.

[0069] Step 3. Liquid release: (1) Move the central tip electrode 1 to above the target container.

[0070] (2) Disconnect the connection between the central tip electrode 1 and the negative electrode (turn off the second switch 10), and the liquid is released to the target container under the action of gravity and electric field force.

[0071] It should be noted that the device of the present invention has the following designs: (1) Design for enhancing the suction efficiency The tip diameter of the central tip electrode 1 is smaller than the tip diameters of the surrounding tip electrodes 2, which helps to increase the electric field strength and enhance the liquid suction ability.

[0072] The spacing between the central tip electrode 1 and the surrounding tip electrodes 2 is designed to be uniform, ensuring the stability of the electric field distribution and improving the smoothness of the liquid suction process.

[0073] (2)Optimization for reducing liquid residue The tip design of the central tip electrode 1 reduces the contact area between the liquid droplet and the tip surface, helping to reduce the liquid adhesion force and thus improve the release efficiency.

[0074] The electric field adjustment mechanism can adapt to different liquid viscosities and optimize the balance between liquid suction and release.

[0075] (3)Relationship between viscosity and suction time The higher the liquid viscosity, the longer the suction time. Optimizing the device structure and operating parameters can further shorten the suction time of high-viscosity liquids.

[0076] (4)Flexibility of dual-switch control The first switch 7: used to control the on / off of the main power supply to ensure operation safety; The second switch 10: flexibly switches the suction and release functions, with simple operation.

[0077] Practical advantages of the structure and function of the present invention: 1. Portable design: The device is powered by a battery, has good mobility, and is suitable for laboratory and on-site operations.

[0078] 2. Multifunctional control: Adjust the voltage through a potentiometer and control the liquid operation with a dual-switch to meet different liquid types and experimental requirements.

[0079] 3. Efficient suction and release: The tip design optimizes the electric field distribution and liquid behavior, enabling fast and stable liquid transfer.

[0080] 4. Wide application range: It can handle liquids with viscosities ranging from low to ultra-high (50 cSt to 30000 cSt), with diverse application scenarios.

[0081] The device of the present invention successfully solves the technical problem of difficult suction and release of high-viscosity liquids (such as silicone oil) through innovative multi-tip design, electric field control, and portable integration, and has high efficiency, stability, and wide applicability. The optimized design can further improve its performance to meet the needs of more complex application scenarios. Figure 4 It can be seen that the suction principle of the device of the present invention is based on the core mechanism of corona discharge driving liquid movement, and through the synergistic action of electric field force and liquid surface tension, the suction of high-viscosity liquids is achieved. The following is a detailed description of the device suction principle:

[0082] As Figure 4 shown in a of (1)Corona discharge forms an ionic wind A voltage (+V) is applied between the central tip electrode (connected to the negative pole) and the surrounding tip electrodes (connected to the high-voltage positive pole), generating a strong electric field between the two types of electrodes. The electric field intensity is highest near the tips, resulting in the occurrence of the corona discharge phenomenon. The corona discharge releases a large number of positive ions, forming an ionic wind. The ionic wind flows from the surrounding tip electrodes towards the central tip electrode under the action of the electric field force, pushing the charges towards the central electrode to concentrate.

[0083] (2) Change in the surface charge distribution of the liquid When an electric field is applied to the liquid surface, a non-conductive liquid (such as silicone oil) generates a charge distribution on the liquid surface due to the polarization effect. The surface charges of the liquid are affected by the ionic wind and the electric field force, gradually approaching the central tip electrode, causing the liquid to rise along the central tip electrode.

[0084] (3) As shown in Figure 4 b in the figure shows the effect of the electrohydrodynamic pressure on the liquid. The driving forces for the liquid to rise are the electric field force and the electrohydrodynamic pressure. The electric field force forms a pressure gradient around the tips, thereby pushing the liquid to move upward. The surface tension of the liquid around the tip electrodes further enhances the rising stability of the liquid. Eventually, the liquid stops rising when the gravity and the electric field force are balanced.

[0085] (4) Regulation of the suction process: The height and volume of the liquid suction are determined by the magnitude of the voltage (+V). A higher voltage enhances the electric field force, sucking more liquid. By precisely regulating the voltage, high-precision control of the liquid suction volume can be achieved.

[0086] The present invention uses the ionic wind generated by corona discharge to promote the polarization of the liquid electrode, and drives the liquid to rise along the central tip electrode through the electric field force. This principle is not only applicable to the suction of high-viscosity non-conductive liquids, but also realizes the precise control of the liquid volume by adjusting the voltage, providing an innovative solution for the efficient transfer of high-viscosity liquids.

[0087] Figure 5 shows the electric field distribution between the tips and the charge distribution on the liquid surface, clearly demonstrating the physical characteristics of the device during operation through simulation. The following is a detailed analysis of the two parts of the simulation:

[0088] Figure 5 a in the figure is the electric field distribution between the tips.

[0089] Electric field intensity distribution: The figure a shows the electric field intensity distribution between the central tip electrode (negative pole) and the surrounding tip electrodes (positive pole). The electric field reaches its maximum value near the central tip electrode (red area, which is the key area where corona discharge occurs). As the distance increases, the electric field intensity rapidly weakens (green and blue areas), indicating that the strong electric field is concentrated in a small area around the tips.

[0090] Function of the tip electrode: A strong electric field gradient is formed between the central tip electrode and the surrounding tip electrodes. This gradient drives the positive ions to flow from the surrounding tip electrodes to the central tip electrode, generating an ionic wind, which in turn pushes the liquid to rise along the central tip electrode.

[0091] Figure 5 In Figure b, it is the charge distribution on the liquid surface.

[0092] Charge density distribution: The charge density on the liquid surface is radially distributed with the central tip electrode as the symmetry point. The charge density reaches its maximum value near the central tip electrode (the red area, about 10 −3 C / m 2 ), indicating that the liquid is highly polarized under the action of the electric field. As the distance from the central tip electrode increases, the charge density gradually decreases (the blue area).

[0093] Influence of charges on the behavior of the liquid: The charges on the liquid surface are driven by the electric field force to concentrate towards the central tip electrode, thus pushing the liquid to move upward. The distribution of the charge density also shows that the action of the electric field force is most significant at the central tip electrode.

[0094] Figure 5 Significance of the simulation (1) Function of the electric field distribution: The concentration of the electric field strength ( Figure 5 a in the figure) ensures that the liquid can be accurately sucked, and the electric field force drives the liquid to rise along the central tip electrode. The distribution of the electric field gradient provides a theoretical basis for the generation of the ionic wind, further verifying the driving force of the ionic wind on the liquid movement.

[0095] (2) Function of the charge distribution: The charge density distribution on the liquid surface illustrates the effectiveness of the electric field in polarizing the liquid, ensuring that the liquid can be sucked even under non-conductive conditions. The concentration of the charges reflects the precise control ability of the electric field force on the liquid suction.

[0096] (3) Basis for the optimized design: The simulation of the electric field and charge distribution can serve as an important basis for optimizing the design of the tip electrode, the tip spacing, and the voltage magnitude, thereby improving the liquid suction efficiency and the performance of the device.

[0097] Through the simulation of the electric field distribution and the charge distribution, the core working principle of this device has been theoretically verified. This simulation not only demonstrates the physical processes of corona discharge and liquid movement but also provides reliable reference data for the further optimization of the device.

[0098] Figure 6For the liquid suction process, the following is a detailed stage - by - stage analysis of this suction process: Experimental background and annotations: The time course (in seconds) and the tip diameter of the central tip electrode (100 µm) are marked in the figure, showing the whole process of the liquid being gradually sucked to the tip from the initial position. Key nodes on the time axis (such as 0.0 s, 6.6 s, 8.1 s, 20.3 s, 54.2 s, and 60.0 s) reflect different stages of the liquid during the suction process.

[0099] Dynamic description of liquid suction in each stage: (1) Initial stage (0.0 s): The central tip electrode just touches the liquid surface, and the electric field has not yet formed an obvious influence. The liquid is in a static state, and no obvious movement or deformation is seen.

[0100] (2) Initial suction stage (6.6 s): Corona discharge begins, and an electric field force is formed in the contact area between the central tip electrode and the liquid. The electric field force drives the liquid to rise gradually, and a small droplet is formed near the tip. The droplet is affected by surface tension, and its shape shows a round arc.

[0101] (3) Rapid suction stage (8.1 s): The rising speed of the liquid significantly increases, and the droplet size gradually enlarges. The driving effect of the ion wind and surface tension act together to make the liquid move steadily towards the middle section of the tip. The liquid has partially separated from the liquid surface and a clear liquid column is formed on the tip.

[0102] (4) Stable rising stage (20.3 s): The liquid further rises along the tip, and the droplet gradually enlarges and approaches the middle section of the central tip electrode. The balance between the electric field force and gravity gradually stabilizes, and the droplet maintains a certain height position.

[0103] (5) Electric field force balance stage (54.2 s): The liquid is completely sucked to the tip, and the droplet reaches its maximum volume. The liquid no longer rises, and gravity and the electric field force reach a dynamic balance.

[0104] (6) Suction completion stage (60.0 s): The liquid suction process is completely over, and the droplet remains in a stable state. At this time, the central electrode can be turned off by adjusting the electric field to discharge the liquid into the target container.

[0105] Key physical mechanisms: (1) Electric field force drive: The electric field force between the tip electrode and the liquid is the core driving force for sucking the liquid. The electric field force overcomes the viscosity and gravity of the liquid, causing the liquid to rise along the central tip electrode.

[0106] (2) Stabilizing effect of surface tension: The surface tension of the liquid plays a role in stabilizing the droplet shape during the suction process. In the rapid suction stage, surface tension limits the excessive expansion of the droplet and prevents it from falling off.

[0107] (3)Balance between gravity and electric field force: When the liquid rises to the middle section of the central tip electrode, the gravity and the electric field force reach equilibrium, and the liquid stops rising.

[0108] Characteristics of the liquid suction process High efficiency: The time for sucking the liquid is short (completed within about 60 seconds), and it can quickly process high-viscosity liquids.

[0109] Controllability: The suction height and the liquid volume can be precisely controlled by adjusting the voltage.

[0110] Applicability: It can handle liquids with different viscosities, especially suitable for high-viscosity liquids (such as silicone oil).

[0111] During the liquid suction process, through the synergistic effect of the ionic wind and the electric field force, the liquid is gradually sucked to the top of the tip electrode. The experimental diagram clearly shows the dynamic process of the liquid from rest to complete suction, verifying the high efficiency and reliability of the liquid driving mechanism based on corona discharge. This process can precisely control the suction volume and is applicable to high-viscosity liquids and microfluidic operation scenarios.

[0112] Figure 7 The following shows the liquid suction effects under different voltage conditions. It can be clearly seen from the figure that under different voltage conditions, the liquid suction effects vary significantly. This indicates that the voltage magnitude directly affects the suction height and volume of the liquid. The following is a detailed analysis of the suction effects at different voltages:

[0113] Experimental background: The liquid is sucked by the electric field force of the central tip electrode, and the electric field intensity under different voltages directly determines the driving force for liquid suction. Figure 7 The results of liquid suction under voltage conditions from 3.8 kV to 5.1 kV are shown. The suction height and the droplet volume gradually increase with the increase of the voltage.

[0114] Analysis of the suction effects at different voltages: (1)3.8 kV: A small droplet is formed on the central tip electrode, with a small volume. The electric field intensity is not sufficient to overcome the gravity and viscous resistance of the liquid, and only initial suction can be formed.

[0115] (2)4.0 kV: The droplet volume slightly increases, indicating that the electric field intensity begins to increase. The liquid starts to rise significantly along the central tip electrode, but the suction height is still limited.

[0116] (3)4.3 kV: The liquid suction height and the droplet volume increase significantly. The electric field intensity gradually increases and can overcome the gravity of more liquid, causing the droplet to further expand.

[0117] (4)4.5 kV: The height and volume of the droplet continue to increase, and the droplet approaches the stable region of the central needle electrode. At this time, the electric field strength is sufficient to drive the liquid to rise stably, but it still has not reached the maximum suction capacity.

[0118] (5)4.7 kV: The liquid suction volume increases significantly, and the volume and height of the droplet approach the upper limit of the suction capacity of the central needle electrode. The electric field force gradually reaches a dynamic equilibrium with the liquid gravity and surface tension, and the droplet shape is stable.

[0119] (6)4.9 kV: The volume of the droplet continues to increase, showing a higher suction capacity. At this time, the electric field force is significantly enhanced, causing the liquid to rise more concentratedly along the central needle electrode, and the droplet tends to be saturated.

[0120] (7)5.1 kV: The liquid suction reaches the maximum volume and height. The shape of the droplet is stable, indicating that the electric field force has reached the maximum suction capacity for the liquid.

[0121] Influence of Voltage on Suction Effect Relationship between suction height and voltage: The liquid suction height shows a non-linear growth trend with the increase of voltage. In the low voltage stage (3.8 kV - 4.5 kV), the liquid suction volume increases rapidly; while in the high voltage stage (4.7 kV - 5.1 kV), the suction volume tends to be saturated.

[0122] Relationship between suction volume and voltage: The volume of the droplet is directly related to the voltage. As the voltage increases, the volume of the droplet gradually increases, indicating that a higher electric field strength can drive more liquid to rise.

[0123] Stability of liquid shape: At low voltages, the droplet volume is small and the shape is stable. At high voltages, the droplet volume increases, but the surface tension balances with the electric field force, and the droplet shape remains stable.

[0124] Application Significance Precise control of suction volume: By adjusting the voltage magnitude, the suction volume (volume) of the liquid can be precisely controlled to meet different experimental requirements.

[0125] High efficiency and flexibility: The device can adapt to the suction requirements of liquids with different viscosities, and the operation can be completed by simply adjusting the voltage.

[0126] Optimization of operation parameters: The simulation results and experimental data show that the voltage range of 4.5 kV - 5.1 kV is the optimal working range for liquid suction.

[0127] The experimental results clearly demonstrate the decisive influence of voltage on the liquid suction process. By controlling the voltage magnitude, the suction height and volume of the liquid can be flexibly adjusted, providing an effective means for the precise control of liquid transfer.

[0128] Figure 8 For the liquid suction effect under different viscosity conditions, it can be clearly observed from the figure that there are significant differences in the liquid suction effect under different viscosity conditions, demonstrating the ability and characteristics of the device in handling liquids with a wide range of viscosities. The following is a detailed analysis of the liquid suction effect for different viscosities:

[0129] Experimental background: The liquid viscosity ranges from 50 cSt (low viscosity) to 3000 cSt (high viscosity), covering typical experiments from aqueous liquids to high-viscosity liquids such as silicone oil. The same electric field conditions (such as voltage and electrode configuration) were used in the tests to observe the height and morphological changes during the liquid suction process.

[0130] Analysis of Liquid Suction Effect for Different Viscosities (1) 50 cSt: The liquid rapidly rises under the action of the electric field force of the central needle tip electrode, forming a stable liquid droplet. The suction height is relatively high, and the liquid droplet morphology is relatively slender, indicating that the low-viscosity liquid has less resistance to movement under the drive of the electric field force.

[0131] (2) 100 cSt: The liquid can rise smoothly and form a relatively large liquid droplet. Compared with 50 cSt, the liquid droplet morphology is slightly fuller, indicating that as the viscosity increases, the electric field force needs to overcome greater internal fluid resistance.

[0132] (3) 500 cSt: The liquid suction height decreases slightly, but the liquid droplet volume increases and the morphology becomes more plump. At this time, the electric field force needs to significantly overcome the viscous resistance of the liquid, and the suction efficiency decreases, but it can still maintain stability.

[0133] (4) 1000 cSt: The liquid droplet suction height further decreases, the volume is relatively stable, and the liquid movement speed slows down. The internal friction and surface tension of the high-viscosity liquid are significantly enhanced, posing higher requirements for the electric field force during the suction process.

[0134] (5) 3000 cSt: The liquid suction height is the lowest, and the liquid droplet volume reaches a relatively stable state. Although the viscosity is high, the device can still successfully suck the liquid, demonstrating the adaptability of this technology to high-viscosity liquids.

[0135] Influence of Viscosity on the Suction Process 1. Suction height: As the liquid viscosity increases, the suction height gradually decreases, indicating that high-viscosity liquids require a stronger electric field force to overcome their internal resistance and gravity.

[0136] 2. Liquid droplet morphology: When the viscosity is low, the liquid droplet morphology is slender and the height is relatively high. When the viscosity is high, the liquid droplet tends to be plump and the height is relatively low, showing the hysteresis effect of high-viscosity liquids under the action of the electric field force.

[0137] 3. Suction efficiency: Liquids with low viscosity have high suction efficiency and fast suction speed. Liquids with high viscosity have a slower suction process, but the stability of the liquid is better, reducing the risk of liquid shedding or irregular movement.

[0138] Application significance Wide applicability: The device can adapt to liquids with viscosities ranging from low to high, and shows strong processing ability especially for high-viscosity liquids.

[0139] Precise control: Even under high-viscosity conditions, the device can still precisely control the suction volume through voltage regulation, making it suitable for experiments and applications with high-precision requirements for liquid volume.

[0140] Innovation: Traditional pipetting devices often perform poorly when dealing with liquids with viscosities exceeding 1000 cSt, while this device effectively solves this problem based on the driving mechanism of electric field force.

[0141] Experimental results show that as the liquid viscosity increases, the suction height and efficiency decrease, but this device can still stably complete the liquid suction operation, demonstrating its adaptability and efficiency for liquids in a wide viscosity range. This performance gives it significant advantages in dealing with high-viscosity liquids (such as lubricating oil, silicone oil, resin, etc.), and is a powerful supplement to traditional pipetting techniques.

[0142] Figure 9 Regarding the effect of the number of surrounding needles around the central needle tip on liquid suction, it can be observed from the figure that the number of surrounding needles around the central needle tip has a significant impact on the liquid suction effect. The following is a detailed analysis of the suction effects for different numbers of surrounding needles (1 needle, 2 needles, 3 needles, and 4 needles):

[0143] Experimental background: The central needle tip is the negative electrode, used to directly contact the liquid and suck the liquid. The number of surrounding needles (positive electrodes) varies from 1 to 4, used to form corona discharge and ionic wind to drive the liquid to move towards the central needle tip. The research objective is to analyze the effects of the number of surrounding needles on the liquid suction height, shape, and stability.

[0144] Analysis of suction effects for different numbers of surrounding needles (1) One needle (single surrounding electrode) Suction effect: The liquid is successfully sucked, but the droplet shape is asymmetric, showing tilt and irregular stretching.

[0145] Reason: The electric field distribution provided by a single surrounding needle is uneven, resulting in insufficient directivity of the electric field force on the liquid. The electric field intensity is concentrated at the position of a single needle tip, and the moving efficiency of the liquid is low.

[0146] Characteristics: Low suction efficiency, irregular droplet shape, and poor applicability.

[0147] (2) Two needle tips Suction effect: The symmetry of the droplet shape is relatively good, the height increases, and the suction volume is significantly improved.

[0148] Reason: The two surrounding needle tips form a symmetric electric field distribution, and the electric field force acts more uniformly on the liquid surface. The driving directionality of the ionic wind is better, and the liquid rises more stably.

[0149] Characteristics: The suction effect is good, but the symmetry is still limited, suitable for liquids with general viscosity.

[0150] (3) Three needle tips Suction effect: The droplet shape is highly symmetric, the suction height further increases, and the droplet volume is large.

[0151] Reason: The three needle tips form a triangular symmetric distribution, and the uniformity and directionality of the electric field are significantly improved.

[0152] The driving range of the ionic wind is wider, and the liquid rises stably and efficiently during the process.

[0153] Characteristics: High suction efficiency, stable droplet shape, suitable for medium and high viscosity liquids.

[0154] (4) Four needle tips Suction effect: The droplet reaches the maximum height and volume, and the shape is highly symmetric and stable.

[0155] Reason: The four needle tips form a square symmetric distribution, and the electric field strength and directionality are further optimized.

[0156] The electric field force acts completely uniformly on the liquid surface, and there is almost no disturbance during the suction process.

[0157] Characteristics: The best suction effect, suitable for high viscosity liquids and applications with high requirements for droplet shape.

[0158] Analysis of the influencing mechanism 1. Electric field uniformity: The more the number of surrounding needle tips, the better the uniformity of the electric field, the more symmetric the electric field force on the liquid surface, and the more stable the liquid rises.

[0159] 2. Driving directionality of ionic wind: As the number of surrounding needle tips increases, the directionality of the ionic wind gradually increases, and the driving efficiency improves.

[0160] 3. Droplet shape and stability: The more the number of surrounding needle tips, the more symmetric the droplet shape, the greater the suction height and volume, and the better the stability of the droplet.

[0161] 4. Application significance One needle tip and two needle tips: Suitable for rapid suction scenarios with low requirements for droplet shape, suitable for operating low viscosity liquids.

[0162] Three-tip and four-tip: Provide the best liquid aspiration effect, suitable for medium to high viscosity liquids, especially in scenarios with strict requirements for droplet morphology and aspiration volume (such as microfluidics, biological sample processing).

[0163] Experimental results show that increasing the number of surrounding tips can significantly improve the liquid aspiration effect. The four-tip configuration exhibits the best droplet morphology and aspiration efficiency, suitable for handling high viscosity liquids or scenarios requiring precise control of droplets.

[0164] Figure 10 In it, a is the liquid transfer process of a traditional pipette, and b~e are the liquid transfer processes using the device of the present invention. The relevant parameters: Describe the dynamic stage of transferring 3000 cSt silicone oil under 5 kV corona discharge. One fine tip is used as the ground electrode, and two tips are used as the discharge electrodes.

[0165] Figure 11 It is the schematic diagram of liquid aspiration and discharge during the liquid transfer process of the device of the present invention. Aspiration: By simultaneously turning on the central tip electrode and the surrounding tip electrodes, charged dielectric fluid is aspirated, such as Figure 11 in a.

[0166] Transfer: After aspiration saturation, first turn off the surrounding tip electrodes to avoid the tip from aspirating the dielectric liquid during the upward movement of the central tip electrode away from the liquid surface. During the transfer process, the central tip electrode does not need to be disconnected. After extraction is completed, the surrounding electrodes can also be turned on again to keep the dielectric droplet in place.

[0167] Discharge: Keep the surrounding tip electrodes on and disconnect the central tip electrode to repel the charged dielectric fluid. The principle is as Figure 11 in b, and the high viscosity dielectric liquid can be quickly repelled to accelerate the discharge.

[0168] Example 1 Aspiration and transfer of low viscosity liquid (50 cSt silicone oil) (1) Composition Device parameters: Diameter of the central tip electrode: 100 µm Diameter of the surrounding tip electrodes: 150 µm Tip spacing: 3 mm Output voltage of the high voltage power supply: 3.8 kV Liquid parameters: Silicone oil, viscosity: 50 cSt.

[0169] (2) Operating steps Turn on the first switch to start the power supply, adjust the potentiometer, and set the voltage to 3.8 kV.

[0170] Vertically insert the central needle tip electrode into the surface of the silicone oil liquid.

[0171] Turn on the second switch, connect the central needle tip electrode to the negative pole, and the liquid is sucked to the central needle tip.

[0172] After the liquid droplet forms a stable state, transfer the device above the target container, turn off the second switch, and the liquid is discharged into the target container under the action of gravity.

[0173] (3) Effect The liquid droplet is quickly sucked, and the sucking time is less than 5 seconds.

[0174] The shape of the liquid droplet is stable, without obvious swinging or falling off.

[0175] The liquid discharging process is smooth without residue.

[0176] Example 2 Sucking and Transferring of High-Viscosity Liquid (30000 cSt Silicone Oil) (1) Composition Device parameters: Diameter of the central needle tip: 100 µm Diameter of the surrounding needle tips: 150 µm Distance between the needle tips: 5 mm Output voltage of the high-voltage power supply: 5.5 kV Liquid parameters: Silicone oil, viscosity: 30000 cSt.

[0177] (2) Operating steps Turn on the first switch to start the power supply, adjust the potentiometer, and set the voltage to 5.5 kV.

[0178] Insert the central needle tip into the liquid surface and keep it stable.

[0179] Turn on the second switch, and the liquid slowly rises to the needle tip under the action of the electric field force.

[0180] After the liquid droplet reaches the maximum suction volume, turn off the first switch, take out the central needle tip electrode from the liquid surface. After extraction, the first switch can be turned on again to keep the dielectric liquid droplet stationary in place.

[0181] Transfer the central needle tip electrode above the target container and turn off the second switch to release the liquid.

[0182] (3) Effect The time to suck the liquid is about 1800 seconds.

[0183] The volume of the liquid droplet is large, but its shape is stable, without falling off or unevenness.

[0184] The liquid is completely discharged without obvious residue Example 3 Continuous aspiration and transfer of multiple liquids (micromanipulation) (1) Composition Device parameters: Central tip diameter: 100 µm Peripheral tip diameter: 150 µm Tip pitch: 3 mm Output voltage of high-voltage power supply: adjustable range 3.8 kV - 5.5 kV Liquid parameters: Liquid 1: 50 cSt silicone oil; Liquid 2: 1000 cSt silicone oil; Liquid 3: 30000 cSt silicone oil.

[0185] (2) Operating steps Turn on the first switch to start the power supply, and adjust the voltage to the appropriate range according to the liquid viscosity: Liquid 1: 3.8 kV Liquid 2: 4.5 kV Liquid 3: 5.5 kV Turn on the second switch, insert the central tip electrode into the surface of each liquid in sequence, and aspirate the liquid droplets respectively.

[0186] After each liquid droplet stabilizes, turn off the first switch to complete the aspiration.

[0187] Transfer the device to above the target container one by one, turn on the first switch, and turn off the second switch to release the liquid.

[0188] (3) Effects The aspiration time of each liquid is related to the viscosity, and the aspiration effect is stable.

[0189] Continuous operation of multiple liquids can be achieved, the device runs stably, and no additional adjustment is required.

[0190] Example 4 Precise quantitative aspiration in liquid mixing experiments (1) Composition Device parameters: Central tip diameter: 100 µm Peripheral tip diameter: 150 µm Tip pitch: 4 mm Output voltage of high-voltage power supply: adjustable range 3.8 kV - 5.5 kV Liquid parameters: Liquid 1: 50 cSt silicone oil (5 µL) Liquid 2: 1000 cSt silicone oil (10 µL) Liquid 3: 30000 cSt silicone oil (15 µL).

[0191] (2)Operation steps Turn on the first switch to start the power supply.

[0192] Adjust the potentiometer according to the preset volume requirements and aspirate the target liquids respectively: Liquid 1: Set the voltage at 3.8 kV and aspirate 5 µL; Liquid 2: Set the voltage at 4.5 kV and aspirate 10 µL; Liquid 3: Set the voltage at 5.5 kV and aspirate 15 µL.

[0193] Release the aspirated liquids into the target container in sequence to complete the liquid mixing.

[0194] (3)Function and effect Precisely aspirate and release liquids with an error less than ±1 µL.

[0195] The liquids are mixed evenly without residue or loss.

[0196] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and its equivalent technologies, these changes and modifications are also intended to be included therein.

Claims

1. A high viscosity liquid pipetting device based on corona discharge, characterized in that: The invention comprises a shell (8), wherein one end of a central needle tip electrode (1) is mounted in the shell (8), one end of a plurality of peripheral needle tip electrodes (2) are mounted around the central needle tip electrode (1), and the other ends of the central needle tip electrode (1) and the peripheral needle tip electrodes (2) extend to the outside of the shell (8); a micro high-voltage power supply (5) is mounted in the shell (8), the central needle tip electrode (1) is electrically connected to the negative electrode of the micro high-voltage power supply (5), and is used to contact liquid and absorb or release liquid; the plurality of peripheral needle tip electrodes (2) are electrically connected to the positive electrode of the micro high-voltage power supply (5), and are used to generate corona discharge and form ion wind to drive the liquid to move along the central needle tip electrode (1); a potentiometer (6) is mounted on the shell (8), and the potentiometer (6) is electrically connected to the micro high-voltage power supply (5), and is used to adjust the output voltage of the micro high-voltage power supply (5).

2. The high viscosity liquid pipetting device based on corona discharge according to claim 1, characterized in that: The liquid has a viscosity of 50 cSt to 30000 cSt and is a non-conductive liquid.

3. The high viscosity liquid pipetting device based on corona discharge according to claim 1, characterized in that: A plurality of peripheral needle tip electrodes (2) are symmetrically distributed around the central needle tip electrode (1) and maintain a consistent spacing with the central needle tip electrode (1).

4. The high viscosity liquid pipetting device based on corona discharge according to claim 3, characterized in that: The diameter of the central needle tip electrode (1) is 100µm, the diameter of the peripheral needle tip electrode (2) is 150µm to 450µm, and the distance between the peripheral needle tip electrode (2) and the central needle tip electrode (1) is 3mm to 15mm.

5. The high viscosity liquid pipetting device based on corona discharge according to claim 1, characterized in that: A mounting piece is provided in the shell (8), one end of a central needle tip electrode (1) is mounted in the middle of the mounting piece, one end of a plurality of peripheral needle tip electrodes (2) are mounted on the mounting piece and are distributed circumferentially around the central needle tip electrode (1), a notch is provided at one end of the shell (8), and the other ends of the central needle tip electrode (1) and the plurality of peripheral needle tip electrodes (2) extend through the notch to the outside of the shell (8).

6. The high viscosity liquid pipetting device based on corona discharge according to claim 1, characterized in that: The device further comprises a second switch (10) and a first switch (7), wherein the second switch (10) and the first switch (7) are mounted on the outside of the housing (8), wherein the second switch (10) is electrically connected to the central needle tip electrode (1) and the negative electrode of the micro high voltage power supply (5) respectively, and is used to control the on-off of the central needle tip electrode (1) and the negative electrode, and the first switch (7) is electrically connected to the positive electrode of the micro high voltage power supply (5) and the surrounding needle tip electrodes (2) respectively, and is used to control the on-off between the surrounding needle tip electrodes (2) and the micro high voltage power supply (5).

7. The high viscosity liquid pipetting device based on corona discharge according to claim 1, characterized in that: The micro high-voltage power supply (5) is electrically connected to a battery (4), a display screen (3) is installed on the outside of the housing (8), and the display screen (3) is electrically connected to the battery (4).

8. The high viscosity liquid pipetting device based on corona discharge according to claim 6, characterized in that: An end of the housing (8) away from the central needle tip electrode (1) is detachably connected to an end cap (9), and the second switch (10) and the first switch (7) are mounted on the end cap (9).

9. A method for transferring high viscosity liquids based on corona discharge, characterized in that: Using the device according to claim 1 to perform pipetting, comprising the following steps: Connect the surrounding needle tip electrode (2) to the positive electrode of the micro high voltage power supply (5), and set the appropriate voltage of the micro high voltage power supply (5) by adjusting the potentiometer (6); The central needle tip electrode (1) is vertically inserted into the liquid surface, and the central needle tip electrode (1) is connected to the negative electrode of the micro high-voltage power supply (5). After the high-voltage electric field is formed, an ion wind is generated by corona discharge, which drives the liquid to rise along the central needle tip electrode (1) to absorb the liquid; After the liquid droplet reaches a stable state, the central needle tip electrode (1) is taken out from the liquid surface and moved above the target container, and the central needle tip electrode (1) is disconnected from the negative electrode of the micro high-voltage power supply (5), so that the absorbed liquid is released from the central needle tip electrode (1) into the target container under the action of gravity and residual electric field force.

10. The method according to claim 9, characterized in that During the liquid absorption process, after the liquid is absorbed to saturation, the surrounding needle tip electrodes (2) are first disconnected from the positive electrode of the micro high-voltage power supply (5), and then the central needle tip electrode (1) is taken out from the liquid surface, and then the surrounding needle tip electrodes (2) are connected to the positive electrode of the micro high-voltage power supply (5) again, and then transferred to the top of the target container.

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