Automatic freezing tube batch storage device and using method thereof

Abstract

The invention discloses an automatic cryopreservation tube batch storage device and a using method thereof. The device comprises a main machine body, a batch packaging opening, a tube body loading unit, a tube body position correcting unit, a tube body storage unit, a tube frame feeding unit and a test tube frame. By designing the shifting wheel type tube body loading unit and the tube body position correcting unit, batch out-of-order cryopreservation tubes are subjected to position correcting one by one, so that the cryopreservation tubes are in a to-be-stored vertical state, then the cryopreservation tubes subjected to position correcting are effectively controlled and output in time through the tube body storage unit and are placed in a test tube rack, and finally, the test tube rack is placed in the test tube rack. The test tube rack feeding unit is matched with the storage state to drive the test tube rack to feed, so that the secondary row of freezing tube empty grooves are located under the tube body output holes, the integration of batch filling, one-by-one position correction, timely output and safe loading of freezing tubes is completed, and the freezing tube storage efficiency is greatly improved; the process is completed in the device, manual interference is not needed, bacterial contamination is blocked to a certain degree, and the safety of follow-up sample storage is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of batch management of cryotubes, and more specifically, relates to an automated cryotube batch shelving device and a method for using the same. Background Art

[0002] As an important carrier for storing biological samples, cryotubes are widely used in the fields of biomedicine, clinical research, genetic testing, etc. With the development of high-throughput technologies, the number of cryotubes in laboratories has increased exponentially. How to efficiently place a large number of cryotubes into test tube racks has become a key link in laboratory automation management. The traditional manual operation method is not only inefficient but also prone to sample misplacement, omission, or contamination due to human errors, seriously affecting the reliability of subsequent experimental data and the safety of samples. Therefore, the development of an automated cryotube batch shelving device is of great significance for improving laboratory work efficiency, reducing labor costs, and reducing operation errors, and also provides technical support for the further development of laboratory automation management.

[0003] Currently, the shelving operation of cryotubes in laboratories mainly relies on manual labor. Specifically, the operator needs to take out the cryotubes one by one from the storage bag or container and manually insert them into the corresponding holes according to the hole position sequence of the test tube rack. Test tube racks usually adopt a standardized design, and common specifications include 96-well, 48-well, etc., which means that the same action needs to be repeated dozens or even hundreds of times for each shelving operation. In addition, to ensure the accurate placement of cryotubes, the operator also needs to carefully check the sample number and the hole position number of the test tube rack, further increasing the complexity and time cost of the operation.

[0004] Although the above-mentioned prior art can complete the task of placing cryotubes, it has the following main defects and improvement directions: (1) This method highly relies on manual operation. The method of manually taking and placing cryotubes one by one is time-consuming and laborious, especially when dealing with a large number of samples, the operator is prone to fatigue and reduce work efficiency. (2) It is difficult to avoid contamination of the tube mouth due to inattention or operation errors during manual operation, which in turn affects the safety of the subsequently stored samples, posing a serious threat to the accuracy and safety of sample management. (3) Considering the efficiency and safety of shelving, an automated cryotube batch shelving device should be proposed to improve the standardization and intelligent level of laboratory operations. Summary of the Invention

[0005] In view of the above defects or improvement requirements of the prior art, the present invention provides an automatic batch racking device for cryotubes and its usage method. By designing a dial-type tube loading unit and a tube alignment unit, the batch of disordered cryotubes can be aligned batch by batch, making the cryotubes in a vertical state waiting to be loaded. Subsequently, through the tube racking unit, the aligned cryotubes can be effectively controlled and output in a timely manner and placed in a test tube rack. Finally, through the cooperation of the tube rack feeding unit and the racking state, the test tube rack is fed, so that the empty slots of the next row of cryotubes are directly below the tube output hole, completing the integrated integration of batch loading, individual alignment, timely output, and safe placement of cryotubes; this process is all completed inside the device without manual intervention in holding the tubes, blocking bacterial contamination to a certain extent and improving the safety of the subsequent stored samples.

[0006] To achieve the above object, according to the first aspect of the present invention, an automatic batch racking device for cryotubes includes:

[0007] A main body with a racking operation chamber opened inside, a batch loading port provided at the top of the main body for placing cryotubes concentratedly loaded, a tube loading unit provided at the bottom of the batch loading port, a tube alignment unit provided at the output end below the tube loading unit, a tube racking unit for placing the cryotubes aligned by the tube alignment unit, a tube rack feeding unit provided below the tube racking unit and performing a linear feeding movement, and a test tube rack installed at the output end of the tube rack feeding unit;

[0008] The tube loading unit includes an isolation cross plate serving as the bottom plate of the batch loading port, tube loading ports vertically penetrating and horizontally arrayed on the isolation cross plate, quantitative loading dials installed in each of the tube loading ports, and a dial driving component for driving the quantitative loading dials to rotate; by controlling the rotation of the quantitative loading dials through the dial driving component, the cryotubes in the batch loading port can be quantitatively introduced into the tube alignment unit;

[0009] The tube alignment unit includes partition plates provided on both sides of the lower surface of each of the tube loading ports, a falling chamber provided between the partition plates, and alignment blocks provided in the falling chamber for blocking the cryotubes from a horizontal posture to a vertical posture;

[0010] The cryotube includes a tube main body and a tube alignment edge provided near the upper end of the tube main body.

[0011] Preferably, the tube rack feeding unit includes:

[0012] A horizontal guide rail horizontally fixed on the bottom surface of the racking operation chamber, a guiding slider slidably installed on the horizontal guide rail, a rack clamping part fixedly connected to the guiding slider for clamping the test tube rack, and a feeding driving unit installed on the rack clamping part and driving it to perform a linear feeding movement.

[0013] Preferably, the feed driving unit includes:

[0014] A driving nut fixed to the clamping part of the frame body, a feed driving screw threadedly and linearly connected to the driving nut, and a power source for driving the feed driving screw to rotate.

[0015] Preferably, the tube body returning unit to the rack includes:

[0016] A first mounting hole formed on the side of the falling cavity and located below the positive position stopper, a tube body alignment unit fixed in the first mounting hole, a tube body output hole and a second mounting hole formed on the other side of the falling cavity and kept at the same height as the first mounting hole, and a tube body clamping and releasing unit fixed in the second mounting hole;

[0017] Push multiple cryotubes that have been correctly positioned and suspended above the positive position stopper forward and aligned to the critical edge of the tube body output hole through the tube body alignment unit. Through the tube body clamping and releasing unit, the cryotubes are clamped and taken to the tube body output hole, and then released and dropped into the test tube rack.

[0018] Preferably, the tube body alignment unit includes:

[0019] A top push driving cylinder, a basic connection block arranged at the front end of the top push driving cylinder, an elastic rod with compressible characteristics installed at the front end of the basic connection block, and an alignment push block.

[0020] Preferably, the tube body clamping and releasing unit includes:

[0021] A tube body transfer air cylinder, and a negative pressure suction head fixed to the front end of the tube body transfer air cylinder for adsorbing cryotubes.

[0022] Preferably, the tube body correct positioning unit includes: a guiding and falling inclined plane arranged on the upper end surface of the positive position stopper.

[0023] Preferably, the number of the tube body loading units is 8 groups or 12 groups.

[0024] Preferably, the rotation trajectory of the blade extending end of the quantitative loading dial exceeds the upper surface of the isolation cross plate.

[0025] According to the second aspect of the present invention, a method for using an automatic cryotube batch returning device to the rack includes the following steps:

[0026] S100: First, load a batch of cryotubes in bags into the batch container opening. Since the cryotubes are made of plastic, they will not be damaged due to collision. At the same time, a part of the cryotubes are longitudinally clamped between the blades of the quantitative loading dial, and the other part of the cryotubes are arranged horizontally and misaligned in the batch container opening;

[0027] S200: Start the tube loading unit, control the quantitative loading thumbwheel to rotate through the thumbwheel driving assembly, and quantitatively introduce the cryopreservation tube stuck between the blades of the quantitative loading thumbwheel into the drop chamber, completing the initial direction alignment; at the same time, since the rotation trajectory of the blade extension end of the quantitative loading thumbwheel exceeds the upper surface of the isolation horizontal plate, it will play a role in aligning the misplaced cryopreservation tubes in the batch loading port, so that they are adjusted from oblique or horizontal to longitudinal and stuck between the blades of the quantitative loading thumbwheel;

[0028] S300: After the cryopreservation tube enters the falling chamber, the secondary alignment is completed under the action of the alignment block, the alignment edge of the tube body of the cryopreservation tube is stuck above the alignment block, and the main body of the tube falls from between the alignment blocks, so that the whole tube is kept in an aligned hanging state;

[0029] S400: Start the tube rack return unit, control the push drive cylinder to push forward, push the cryotube in the upright hanging state forward to the front end of the negative pressure suction head, then control the negative pressure suction head to adsorb the cryotube, start the tube transport cylinder to drag the cryotube into the tube output hole, then control the negative pressure suction head to release the cryotube, so that the cryotube falls to the tube hole of the test tube rack below;

[0030] S500: When the front row of empty slots of the test tube rack are loaded with cryotubes, the feed drive screw is controlled to rotate, thereby driving the drive nut to move linearly, and then the rack clamping part is controlled to carry the test tube rack forward, so that the empty slots of the second row of cryotubes are directly below the output holes of the tube body, waiting for loading;

[0031] S600: After the test tube rack is assembled, it is removed from the clamping part of the rack body and replaced with a new test tube rack, and the above steps are repeated to assemble the cryopreserved tubes of the new test tube rack.

[0032] In general, the above technical solutions conceived by the present invention can achieve the following beneficial effects compared with the prior art:

[0033] 1. An automated batch cryopreservation tube racking device of the present invention realizes batch-by-batch alignment of cryopreservation tubes in disorder by designing a thumbwheel tube loading unit and a tube alignment unit, so that the cryopreservation tubes are in a vertical state to be arranged for arrangement, and then the aligned cryopreservation tubes are timely and effectively controlled to be output through the tube racking unit and loaded into the test tube rack, and finally, the test tube rack is driven to feed by the tube rack feeding unit in coordination with the arrangement state, so that the empty slots of the second row of cryopreservation tubes are directly below the output holes of the tube bodies, thus completing the integrated integration of batch loading, one-by-one alignment, timely output and safe loading of cryopreservation tubes; the process is all completed inside the device without the need for manual intervention of holding the tubes, thus blocking bacterial contamination to a certain extent and improving the safety of subsequent sample loading.

[0034] 2. In the present invention, an automated batch-replacing device for cryopreservation tubes has a rotation trajectory of the extended end of the blade of the quantitative loading dial wheel that exceeds the upper surface of the isolation horizontal plate. This can adjust the misplaced cryopreservation tubes in the batch loading port to the correct position, so that the tubes are adjusted from an oblique or horizontal direction to a vertical direction and are inserted between the blades of the quantitative loading dial wheel, thereby improving the correct position effect to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the overall front view structure of an automated cryopreservation tube batch racking device according to an embodiment of the present invention;

[0036] Figure 2 This is a schematic diagram of a tube loading unit of an automated cryopreservation tube batch racking device according to an embodiment of the present invention in a first working state from a front view;

[0037] Figure 3 This is a schematic diagram of a tube loading unit of an automated cryopreservation tube batch racking device according to an embodiment of the present invention in a second working state from a front view;

[0038] Figure 4 This is a schematic diagram of a tube loading unit of an automated cryopreservation tube batch racking device according to an embodiment of the present invention in a front view in a third working state;

[0039] Figure 5 This is a schematic diagram of the overall side view of an automated cryopreservation tube batch racking device according to an embodiment of the present invention;

[0040] Figure 6 This is a schematic diagram of the structure of a tube racking unit of an automated cryopreservation tube batch racking device according to an embodiment of the present invention;

[0041] Figure 7 The present invention is a flowchart of a method for using an automated cryopreservation tube batch racking device according to an embodiment of the present invention.

[0042] In all the drawings, the same reference numerals denote the same technical features, specifically: 1 - main body, 100 - racking operation chamber, 2 - batch container loading port, 3 - tube loading unit, 300 - isolation cross plate, 301 - tube loading port, 310 - quantitative loading dial, 4 - tube alignment unit, 401 - partition plate, 402 - falling chamber, 403 - alignment stop, 404 - guiding inclined surface, 5 - tube rack feeding unit, 501 - horizontal guide rail, 502 - guiding slider, 510 - feeding drive unit, 511 - feeding drive screw, 512 - drive nut, 520 - rack clamping part, 6 - cryotube, 601 - tube main body, 602 - tube alignment edge, 7 - test tube rack, 8 - tube racking unit, 810 - tube alignment unit, 811 - push drive cylinder, 812 - base connection block, 813 - elastic rod, 814 - alignment push block, 820 - tube clamping and releasing unit, 821 - negative pressure suction head, 822 - tube transfer cylinder, 830 - tube output hole. Detailed implementation manners

[0043] In the description of the present application, it should be understood that the orientation or positional relationships indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention.

[0044] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise specifically defined.

[0045] In the present application, unless otherwise clearly specified and defined, the terms "installed", "connected", "connected to", "fixed", etc. should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0047] As Figures 1 to 6 shown, in an embodiment of the present invention, the automated cryotube batch shelving device includes:

[0048] A main body 1 with a shelving operation chamber 100 opened inside, a batch loading port 2 provided at the top of the main body 1 for placing cryotubes 6 loaded centrally, a tube loading unit 3 provided at the bottom of the batch loading port 2, a tube alignment unit 4 provided at the output end below the tube loading unit 3, a tube shelving unit 8 for aligning and placing the cryotubes 6 aligned by the tube alignment unit 4, a tube rack feeding unit 5 provided below the tube shelving unit 8 and performing a linear feeding movement, and a test tube rack 7 installed at the output end of the tube rack feeding unit 5;

[0049] The tube loading unit 3 includes an isolation cross plate 300 serving as the bottom plate of the batch loading port 2, tube loading ports 301 vertically penetrating and horizontally arranged in the isolation cross plate 300, quantitative loading pulleys 310 installed in each of the tube loading ports 301, and a pulley driving assembly for driving the quantitative loading pulleys 310 to rotate; by controlling the rotation of the quantitative loading pulleys 310 through the pulley driving assembly, the cryotubes 6 in the batch loading port 2 are quantitatively introduced into the tube alignment unit 4;

[0050] The tube alignment unit 4 includes partition plates 401 provided on both sides of the lower surface of each of the tube loading ports 301, a falling cavity 402 provided between the partition plates 401, and a positioning block 403 provided in the falling cavity 402 for blocking and dropping the cryotube from a horizontal posture to a vertical posture;

[0051] The cryotube 6 includes a tube main body 601 and a tube alignment edge 602 provided near the upper end of the tube main body 601.

[0052] In the embodiment of the present invention, by designing a dial-type tube loading unit and a tube alignment unit, the batch-by-batch alignment of a batch of disordered cryotubes is realized, so that the cryotubes are in a vertical state waiting to be loaded. Subsequently, through the tube rack feeding unit 5, the aligned cryotubes are effectively controlled and output in a timely manner and placed in the test tube rack 7. Finally, through the cooperation of the tube rack feeding unit 5 and the loading state, the test tube rack 7 is fed, so that the empty slot of the secondary row of cryotubes 6 is directly below the tube output hole 830, completing the integrated integration of batch loading, one-by-one alignment, timely output, and safe placement of cryotubes; this process is all completed inside the device, without manual interference in handling the tubes, blocking bacterial contamination to a certain extent and improving the safety of the subsequent stored samples.

[0053] As Figure 1 shown, in the embodiment of the present invention, the tube rack feeding unit 5 includes:

[0054] A horizontal guide rail 501 horizontally fixed to the bottom surface of the racking operation chamber 100, a guiding slider 502 slidably mounted on the horizontal guide rail 501, a rack clamping part 520 fixedly connected to the guiding slider 502 to clamp the rack of the test tube rack 7, and a feeding driving unit 510 mounted on the rack clamping part 520 and driving it to perform a linear feeding motion.

[0055] As Figure 1 shown, in the embodiment of the present invention, the feeding driving unit 510 includes:

[0056] A driving nut 512 fixed to the rack clamping part 520, a feeding driving screw 511 threadedly and linearly drivingly connected to the driving nut 512, and a power source for driving the feeding driving screw 511 to rotate.

[0057] As Figure 1 、 Figure 5 and Figure 6 shown, in the embodiment of the present invention, the tube racking unit 8 includes:

[0058] A first mounting hole opened on the side surface of the falling cavity 402 and located below the alignment stopper 403, a tube alignment unit 810 fixed in the first mounting hole, a tube output hole 830 and a second mounting hole opened on the other side surface of the falling cavity 402 and kept at the same height as the first mounting hole, and a tube clamping and placing unit 820 fixed in the second mounting hole;

[0059] Through the tube alignment unit 810, multiple cryotubes 6 that have been aligned and suspended above the alignment stopper 403 are pushed forward and aligned to the critical edge of the tube output hole 830. Through the tube clamping and placing unit 820, the cryotubes 6 are clamped and taken to the tube output hole 830 and released to fall into the test tube rack 7.

[0060] As Figure 5and Figure 6 As shown, in the embodiment of the present invention, the tube body has a unit 810, including:

[0061] A push driving cylinder 811, a basic connecting block 812 arranged at the front end of the push driving cylinder 811, an elastic rod 813 with compressible characteristics installed at the front end of the basic connecting block 812, and a push block 814 therefor.

[0062] like Figure 1 , Figure 5 and Figure 6 As shown, in this embodiment of the present invention, the tube clamping unit 820 includes:

[0063] The tube body transport cylinder 822 is fixed to the front end of the tube body transport cylinder 822 and is used to adsorb the negative pressure suction head 821 of the cryopreservation tube 6.

[0064] like Figure 4 As shown, in the embodiment of the present invention, the tube body alignment unit 4 includes: a guide slope 404 provided on the upper end surface of the alignment stopper 403 .

[0065] like Figure 1 As shown, in the embodiment of the present invention, the tube body loading units 3 are 8 groups or 12 groups.

[0066] like Figure 1 and Figure 3 As shown, in the embodiment of the present invention, the rotation trajectory of the blade extension end of the quantitative loading dial wheel 310 exceeds the upper surface of the isolation horizontal plate 300.

[0067] In the embodiment of the present invention, since the rotation trajectory of the protruding end of the blade of the quantitative loading dial wheel 310 exceeds the upper surface of the isolation cross plate 300, it will play a role in adjusting the misplaced cryogenic storage tubes 6 in the batch loading port 2 to the vertical direction so that they can be adjusted from the oblique or horizontal direction to the vertical direction and inserted between the blades of the quantitative loading dial wheel 310, thereby improving the alignment effect to a certain extent.

[0068] like Figure 7 As shown, in an embodiment of the present invention, a method for using an automated cryopreservation tube batch racking device includes:

[0069] S100: First, the bagged batch cryopreservation tubes 6 are loaded into the batch loading port 2. Since the cryopreservation tubes 6 are made of plastic, they will not be damaged by collision. At the same time, a portion of the cryopreservation tubes 6 are longitudinally inserted between the blades of the quantitative loading dial wheel 310, and the other portion of the cryopreservation tubes 6 are transversely staggered and arranged in the batch loading port 2.

[0070] S200: Start the tube loading unit 3, control the quantitative loading dial 310 to rotate through the dial driving assembly, and quantitatively introduce the cryotube 6 stuck between the blades of the quantitative loading dial 310 into the falling chamber 402, completing the initial direction alignment; at the same time, since the rotation trajectory of the blade extension end of the quantitative loading dial 310 exceeds the upper surface of the isolation horizontal plate 300, it will play a role in aligning the misplaced cryotube 6 in the batch container port 2, so that it is adjusted from the oblique or horizontal direction to the longitudinal direction and stuck between the blades of the quantitative loading dial 310;

[0071] S300: After the cryopreservation tube 6 enters the falling chamber 402, the secondary alignment is completed under the action of the alignment block 403. The alignment edge 602 of the cryopreservation tube 6 is stuck above the alignment block 403, and the tube body 601 falls from between the alignment blocks 403, so that the whole tube is kept in an aligned hanging state;

[0072] S400: Start the tube return unit 8, control the push drive cylinder 811 to push forward, push the cryotube 6 in the upright hanging state forward to the front end of the negative pressure suction head 821, then control the negative pressure suction head 821 to absorb the cryotube 6, and start the tube transport cylinder 822 to drag the cryotube 6 into the tube output hole 830, then control the negative pressure suction head 821 to release the cryotube 6, so that the cryotube 6 falls to the tube hole of the test tube rack 7 below;

[0073] S500: After the cryotube 6 is loaded into the front empty slot of the test tube rack 7, the feeding drive screw 511 is controlled to rotate, thereby driving the driving nut 512 to move linearly, and then the rack clamping part 520 is controlled to carry the test tube rack 7 forward, so that the empty slot of the second row of cryotubes 6 is directly below the tube output hole 830, waiting for loading;

[0074] S600: After the test tube rack 7 is assembled, it is removed from the rack clamping portion 520 and replaced with a new test tube rack 7 . The above steps are repeated to assemble the cryopreservation tubes 6 of the new test tube rack 7 .

[0075] The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the present application. The above are only preferred implementations of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present application, and these improvements and variations should also be regarded as the protection scope of the present application.

Claims

1. An automated cryopreservation tube batch racking device, characterized in that: include: A main body (1) having a rack return operation chamber (100) provided therein, a batch assembly port (2) provided at the top of the main body (1) for placing cryopreservation tubes (6) to be loaded in batches, a tube loading unit (3) provided at the bottom of the batch assembly port (2), a tube alignment unit (4) provided at the output end below the tube loading unit (3), a tube rack return unit (8) for placing the cryopreservation tubes (6) aligned by the tube alignment unit (4), a tube rack feeding unit (5) provided below the tube rack return unit (8) and performing a linear feeding motion, and a test tube rack (7) installed at the output end of the tube rack feeding unit (5); The tube loading unit (3) comprises an isolation horizontal plate (300) serving as a bottom plate of the batch container port (2), tube loading ports (301) vertically penetrating and arranged in a horizontal array on the isolation horizontal plate (300), quantitative loading thumbwheels (310) installed in each of the tube loading ports (301), and a thumbwheel driving assembly for driving the quantitative loading thumbwheels (310) to rotate; the thumbwheel driving assembly controls the rotation of the quantitative loading thumbwheels (310) so as to quantitatively introduce the cryopreservation tubes (6) in the batch container port (2) into the tube alignment unit (4); The tube body correcting unit (4) comprises cavity partition plates (401) arranged on both sides of the lower surface of each tube body loading port (301), a drop cavity (402) arranged between the cavity partition plates (401), and a correcting block (403) arranged in the drop cavity (402) for stopping the cryopreservation tube from a horizontal posture to a vertical posture; The cryopreservation tube (6) comprises a tube body (601) and a tube body aligning edge (602) arranged near the upper end of the tube body (601).

2. The automatic cryopreservation tube batch storage device according to claim 1, characterized in that: The pipe rack feeding unit (5) comprises: A horizontal guide rail (501) horizontally fixed to the bottom surface of the rack return operation chamber (100), a guide slider (502) slidably mounted on the horizontal guide rail (501), a rack clamping portion (520) fixedly connected to the guide slider (502) to clamp the test tube rack (7), and a feed drive unit (510) mounted on the rack clamping portion (520) and driving it to perform linear feed motion.

3. The automated cryopreservation tube batch storage device according to claim 1, characterized in that: The feed drive unit (510) comprises: A driving nut (512) fixed to the frame clamping portion (520), a feed driving screw (511) threadedly linearly connected to the driving nut (512), and a power source for driving the feed driving screw (511) to rotate.

4. The automated cryopreservation tube batch storage device according to claim 1, characterized in that: The tube body frame unit (8) comprises: A first mounting hole opened on the side of the falling cavity (402) and located below the positive stopper (403), a tube alignment unit (810) fixed in the first mounting hole, a tube output hole (830) maintained at the same height as the first mounting hole and opened on the other side of the falling cavity (402), a second mounting hole, and a tube clamping unit (820) fixed in the second mounting hole; The plurality of cryogenic tubes (6) that have been suspended in the correct position on the correct position stopper (403) are pushed forward to be aligned to the critical edge of the tube output hole (830) through the tube body alignment unit (810), and the cryogenic tubes (6) are clamped to the tube output hole (830) through the tube body clamping unit (820), and then released to fall into the test tube rack (7).

5. The automatic cryopreservation tube batch storage device according to claim 4, characterized in that: The tube body has a unit (810) comprising: A push-driving cylinder (811), a basic connection block (812) arranged at the front end of the push-driving cylinder (811), an elastic rod (813) with compressible characteristics installed at the front end of the basic connection block (812), and a push-driving block (814) therefor.

6. The automated cryopreservation tube batch storage device according to claim 5, characterized in that: The tube clamping and placing unit (820) comprises: The tube transport cylinder (822) is fixed to the front end of the tube transport cylinder (822) and is used for adsorbing the negative pressure suction head (821) of the cryopreservation tube (6).

7. The automated cryopreservation tube batch storage device according to claim 1, characterized in that: The tube body alignment unit (4) comprises: a guide slope (404) provided on the upper end surface of the alignment stopper (403).

8. The automated cryopreservation tube batch storage device according to claim 1, characterized in that: The tube body loading units (3) are 8 groups or 12 groups.

9. The automatic cryopreservation tube batch storage device according to claim 1, characterized in that: The rotation track of the blade extension end of the quantitative loading dial wheel (310) exceeds the upper surface of the isolation horizontal plate (300).

10. The method for using the automated cryopreservation tube batch racking device according to claim 1, characterized in that: The steps include: S100: First, the bagged batch cryopreservation tubes (6) are loaded into the batch assembly port (2). Since the cryopreservation tubes (6) are made of plastic, they will not be damaged by collision. At the same time, a portion of the cryopreservation tubes (6) are longitudinally inserted between the blades of the quantitative loading dial wheel (310), and another portion of the cryopreservation tubes (6) are transversely staggered and arranged in the batch assembly port (2); S200: Start the tube loading unit (3), control the quantitative loading dial (310) to rotate through the dial driving assembly, and quantitatively introduce the cryotube (6) stuck between the blades of the quantitative loading dial (310) into the drop chamber (402), completing the initial directional alignment; at the same time, because the rotation trajectory of the blade extension end of the quantitative loading dial (310) exceeds the upper surface of the isolation horizontal plate (300), it will play a role in aligning the misplaced cryotube (6) in the batch container port (2), so that it is adjusted from the oblique or horizontal direction to the longitudinal direction and stuck between the blades of the quantitative loading dial (310); S300: After the cryopreservation tube (6) enters the falling chamber (402), the secondary alignment is completed under the action of the alignment block (403), the alignment edge (602) of the cryopreservation tube (6) is clamped above the alignment block (403), and the tube body (601) falls from between the alignment blocks (403), so that the whole tube is kept in an aligned hanging state; S400: Start the tube return unit (8), control the push drive cylinder (811) to push forward, push the cryotube (6) in the upright hanging state forward to the front end of the negative pressure suction head (821), then control the negative pressure suction head (821) to absorb the cryotube (6), and start the tube transfer cylinder (822) to drag the cryotube (6) into the tube output hole (830), then control the negative pressure suction head (821) to release the cryotube (6), so that the cryotube (6) falls to the tube hole of the test tube rack (7) below; S500: After the front row of empty slots of the test tube rack (7) are loaded with cryotubes (6), the feed drive screw (511) is controlled to rotate, thereby driving the drive nut (512) to move linearly, and then controlling the rack clamping part (520) to carry the test tube rack (7) forward, so that the empty slots of the second row of cryotubes (6) are located directly below the tube output hole (830) to wait for loading; S600: When the test tube rack (7) is assembled, it is removed from the rack clamping portion (520) and replaced with a new test tube rack (7). The above steps are repeated to assemble the cryopreservation tubes (6) of the new test tube rack (7).

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