High performance liquid chromatography detection method of glycosylated hemoglobin for pregnant women with diabetes

Abstract

The invention relates to the technical field of spectral component measurement, in particular to a high performance liquid chromatography detection method of glycosylated hemoglobin for a diabetic pregnant woman. According to the method, high performance liquid chromatograms at different temperatures are obtained, the chromatograms are processed through EMD mode decomposition, screening is carried out according to peak value characteristics, and the IMF component of the hemoglobin component is determined. Matching the IMF components of the hemoglobin components at different temperatures, and obtaining the hemoglobin type of each IMF component of the hemoglobin components according to the obtained temperature matching components and the retention time of the middle components. And counting the content of each hemoglobin type at all temperatures to obtain the content of each hemoglobin type in the blood sample. According to the method, errors in detection are eliminated through chromatographic detection and data statistics, and a more accurate detection result of glycosylated hemoglobin of a diabetic pregnant woman is obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of spectral component measurement, and particularly relates to a high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes. Background Art

[0002] Pregnant women with diabetes face many health risks during pregnancy. In particular, poor blood glucose control may lead to maternal and fetal complications. Accurately monitoring the glycated hemoglobin level is crucial for evaluating the blood glucose control of pregnant women; Glycated hemoglobin is the product of a continuous and irreversible non-enzymatic binding reaction between hemoglobin in red blood cells and glucose monosaccharides. The main stable product is glycated hemoglobin, which is basically consistent with the lifespan of red blood cells in the body and reflects the average blood glucose level of the patient in the last two to three months; Moreover, this index is not easily affected by the collection time, diet, and changes in the body's insulin level, and thus can be used as an index for diabetes diagnosis.

[0003] High performance liquid chromatography is a common method for detecting glycated hemoglobin in clinical laboratories at present. It determines the type and content of hemoglobin by detecting the peak distribution in the chromatogram. However, because the protein level of glycated hemoglobin in pregnant women with diabetes is usually relatively high, resulting in a wider peak shape and tailing in the chromatogram, making the peaks of glycated hemoglobin overlap with those of other hemoglobins. In order to avoid the influence of peak overlap on the detection results in the prior art, gradient elution, a chromatographic separation technique, is used to separate the peak information of glycated hemoglobin from that of other hemoglobins. However, in the actual use process, because the UV detector is easily affected by temperature, unstable temperature will affect the sensitivity of the detector, resulting in the accuracy of the detection results being affected. Summary of the Invention

[0004] In order to solve the technical problems in the prior art that the peaks of hemoglobin are prone to overlap during the high performance liquid chromatography detection process and the peak separation is inaccurate due to the influence of temperature, the purpose of the present invention is to provide a high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes, and the specific technical solutions adopted are as follows:

[0005] The present invention provides a high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes, and the method includes:

[0006] Obtain a blood sample of a pregnant woman with diabetes, and obtain the high performance liquid chromatograms of the pretreated blood sample at different temperatures;

[0007] For each high performance liquid chromatogram, perform EMD decomposition on the high performance liquid chromatogram to obtain a number of IMF components, and screen out the IMF components of hemoglobin components according to the peak feature obviousness of the IMF components;

[0008] For each IMF component of the hemoglobin component, match the IMF component of the hemoglobin component with each IMF component of the hemoglobin component at different temperatures to obtain a set of temperature-matched components for each IMF component of the hemoglobin component; each IMF component of the hemoglobin component in the set of temperature-matched components corresponds to a temperature; the basis for the matching is to match according to the difference in the peak feature distinctness and the retention time between the IMF components of the hemoglobin component.

[0009] For each IMF component of the hemoglobin component, obtain the hemoglobin type of each IMF component of the hemoglobin component according to the retention time of each component in the set of temperature-matched components; count the peak areas of the IMF components of each hemoglobin type at different temperatures to obtain the content of each hemoglobin type in the blood sample.

[0010] Further, the method for obtaining the peak feature distinctness includes:

[0011] Take the peak with the largest signal value at the peak point in the IMF component as the target peak, and obtain the peak feature distinctness according to the signal value at the peak point, the wavelength range, and the peak symmetry of the target peak.

[0012] Further, the screening method for the IMF component of the hemoglobin component includes:

[0013] Among all the IMF components at a temperature, take the IMF components with the peak feature distinctness greater than the preset screening threshold as the IMF components of the hemoglobin component.

[0014] Further, the matching process includes:

[0015] Optionally select a temperature as the target temperature, take an IMF component of the hemoglobin component at the target temperature as the target component, and sequentially take each IMF component of the hemoglobin component at other temperatures as the comparison component;

[0016] Obtain a matching index according to the difference in the peak feature distinctness and the retention time between the target component and the comparison component; at another temperature, take the comparison component corresponding to the largest matching index as the temperature-matched component of the target component at the other temperature; obtain the temperature-matched components of the target component at all other temperatures to obtain the set of temperature-matched components of the target component.

[0017] Further, the method for obtaining the matching index includes:

[0018] Multiply the difference in the peak feature distinctness by the difference in the retention time, perform negative correlation mapping and normalization to obtain the matching index.

[0019] Further, the method for determining the hemoglobin type of each hemoglobin component IMF component includes:

[0020] Obtain the standard retention time of each hemoglobin type at each temperature;

[0021] Optionally select a hemoglobin type as the target type. For a hemoglobin component IMF component, obtain the retention time difference between the retention time of each component in the temperature-matched component set of the protein component IMF component and the standard retention time at the corresponding temperature of the target type, and take the average retention time difference as the type discrimination degree between the hemoglobin component IMF component and the target type;

[0022] Obtain the type discrimination degree between each hemoglobin component IMF component and each hemoglobin type, and obtain the hemoglobin type of each hemoglobin component IMF component according to the type discrimination degree.

[0023] Further, the obtaining of the content of each hemoglobin type in the blood sample includes:

[0024] At each temperature, take the peak area of the target peak of the hemoglobin component IMF component of each hemoglobin type as the target area, and obtain the area proportion of the target area of each hemoglobin type among all the target areas;

[0025] For each hemoglobin type, take the average value of the area proportions at all temperatures as the content of the hemoglobin type.

[0026] Further, the pretreatment process of the blood sample includes:

[0027] Centrifuge the blood sample for separation, take the supernatant, add a protein remover, and after obtaining the protein-removed sample, dilute the sample, select a separation C18 column, and prepare 20% acetonitrile and 80% phosphate buffer solution.

[0028] Further, the method for obtaining the peak symmetry includes:

[0029] The peak point of the target peak divides the target peak into two peak regions, and the peak symmetry is obtained according to the area difference between the two peak regions.

[0030] Further, for each hemoglobin component IMF amount, select the hemoglobin type with the smallest type discrimination degree as the hemoglobin type of the hemoglobin component IMF amount.

[0031] The present invention has the following beneficial effects:

[0032] In view of the fact that the chromatographic information corresponding to different hemoglobin components corresponds to different signal characteristics in the high-performance liquid chromatography (HPLC) chromatogram, the present invention uses the empirical mode decomposition (EMD) method to decompose the HPLC chromatogram. Each intrinsic mode function (IMF) component can characterize a substance. And after the blood sample is pretreated, hemoglobin is retained as much as possible. The more prominent the peak feature of the IMF component, the more likely the corresponding IMF component is the IMF component of the hemoglobin component, thereby effectively classifying and screening out the IMF components of the hemoglobin component. In order to avoid detection errors at a fixed temperature, the present invention sets multiple temperature groups and conducts statistical analysis. Since there are multiple IMF components of hemoglobin at each temperature, to ensure the accuracy of the statistical results, the present invention determines the temperature matching component set of each IMF component of the hemoglobin component through a matching method, and then determines the hemoglobin type of each IMF component of the hemoglobin component according to the retention time in the temperature matching component set. After determining the hemoglobin type, the content of each hemoglobin type can be determined according to the peak area of the component. Based on modal decomposition and combined with comparative statistics at multiple temperatures, the present invention screens out effective hemoglobin components while avoiding the influence of temperature on detection, improving the detection accuracy of the HPLC detection method for glycated hemoglobin in pregnant women with diabetes. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the technical solutions and advantages in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0034] Figure 1 It is a flowchart of a high-performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes provided by an embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0035] In order to further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following, in combination with the accompanying drawings and preferred embodiments, details the specific implementation manners, structures, features, and effects of a high-performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes proposed according to the present invention. In the following description, different "one embodiment" or "another embodiment" do not necessarily refer to the same embodiment. In addition, the specific features, structures, or characteristics in one or more embodiments can be combined in any suitable form.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs.

[0037] The following specifically describes the specific solution of a high performance liquid chromatography detection method for glycated hemoglobin of pregnant women with diabetes provided by the present invention in conjunction with the accompanying drawings.

[0038] Please refer to Figure 1 , which shows a flowchart of a high performance liquid chromatography detection method for glycated hemoglobin of pregnant women with diabetes provided by an embodiment of the present invention. The method includes:

[0039] Step S1: Obtain a blood sample of a pregnant woman with diabetes, and obtain high performance liquid chromatography diagrams of the pretreated blood sample at different temperatures.

[0040] In the embodiment of the present invention, first collect a blood sample from the arm vein of a pregnant woman with diabetes. The blood sample contains other substances besides hemoglobin. In order to avoid the influence of other substances on the detection, it is necessary to pretreat the blood sample before performing high performance liquid chromatography detection.

[0041] In the embodiment of the present invention, the pretreatment process of the blood sample includes: centrifugally separating the blood sample, taking the supernatant, adding a protein removing agent, diluting the sample after obtaining the protein-removed sample, selecting a C18 separation column, and preparing 20% acetonitrile and 80% phosphate buffer solution.

[0042] After pretreating the blood sample, different temperature environments can be set to obtain high performance liquid chromatography diagrams at each temperature environment. In the embodiment of the present invention, a detection group is set at each temperature between 20 degrees and 40 degrees. Place the pretreated blood sample in an incubator, inject the pretreated blood sample into the HPLC system through an autoinjector, and obtain the high performance liquid chromatography diagram of the sample under each temperature condition through a UV-Vis detector. That is, there is a high performance liquid chromatography diagram at each temperature.

[0043] Step S2: For each high performance liquid chromatography diagram, perform EMD decomposition on the high performance liquid chromatography diagram to obtain a number of IMF components, and screen out the IMF component of the hemoglobin component according to the peak feature obviousness of the IMF components.

[0044] In the high-performance liquid chromatography (HPLC) chromatogram at each temperature, the chromatographic information is the combined information of multiple substances. To accurately detect the content of hemoglobin, it is necessary to separate the chromatographic information of different hemoglobin types. Moreover, although the blood sample has been pre-treated, the chromatographic information of other substances will still be retained in the chromatogram. Since different hemoglobin types exhibit different chromatographic characteristics in the chromatogram, and hemoglobin types, as the main substances, have more obvious signal characteristics compared to the chromatographic information of other substances. Therefore, in the embodiments of the present invention, the HPLC chromatogram is first subjected to empirical mode decomposition (EMD) to obtain a number of intrinsic mode function (IMF) components. That is, each IMF component represents a mode of information and can represent a substance.

[0045] Due to the relatively large molecular structure scale of hemoglobin and its relatively high content in the pre-treated blood sample, the corresponding IMF component will exhibit characteristics of larger amplitude, wider wavelength range, and more obvious peaks. In contrast, other substances have relatively smaller amplitudes, shorter wavelength ranges, and more irregular peaks. Therefore, the IMF components of hemoglobin can be screened according to the peak feature distinctness in the IMF classification. That is, the more distinct the peak feature, the more likely the corresponding component is an IMF component of hemoglobin.

[0046] Preferably, in an embodiment of the present invention, the method for obtaining the peak feature distinctness includes:

[0047] Taking the peak with the largest peak point signal value in the IMF component as the target peak, and obtaining the peak feature distinctness according to the peak point signal value, wavelength range, and peak symmetry of the target peak. That is, the larger the peak point signal value, wavelength range, and peak symmetry of the target peak, the more obvious the target peak, the greater the peak feature distinctness, and the more likely this IMF component is an IMF component of hemoglobin.

[0048] Further, in an embodiment of the present invention, the method for obtaining the peak symmetry includes:

[0049] The peak point of the target peak divides the target peak into two peak regions, and the peak symmetry is obtained according to the area difference between the two peak regions. That is, the smaller the area difference between the peak regions, the greater the peak symmetry. In the embodiments of the present invention, the absolute value of the difference in area between the two peak regions is subjected to a negative correlation mapping to obtain the peak symmetry. The method of negative correlation mapping is to take the opposite of the absolute value of this difference as the power of an exponential function with the natural constant as the base, and the mapping result of the function is the peak symmetry.

[0050] In an embodiment of the present invention, after normalizing the product of the peak point signal value, wavelength range, and peak symmetry of the target peak, the peak feature distinctness is obtained.

[0051] It should be noted that the normalization method in the embodiments of the present invention can be selected by those skilled in the art. It is a basic mathematical means well-known to those skilled in the art. Methods such as sigmoid function mapping, hyperbolic tangent function mapping, and range normalization can be used for normalization, and no further elaboration and limitation will be made here.

[0052] In an embodiment of the present invention, among all IMF components at a certain temperature, the IMF components with the peak feature distinctness greater than the preset screening threshold are used as the hemoglobin component IMF components. In the embodiments of the present invention, after normalizing the peak feature distinctness, the screening threshold is set to 0.85.

[0053] Step S3: For each hemoglobin component IMF component, match the hemoglobin component IMF component with each hemoglobin component IMF component at different temperatures to obtain a temperature matching component set for each hemoglobin component IMF component; each hemoglobin component IMF component in the temperature matching component set corresponds to a temperature; the basis for matching is to match according to the difference in peak feature distinctness and retention time between hemoglobin component IMF components.

[0054] In step S2, the hemoglobin component IMF components are screened out, but it is not clear which hemoglobin types these components belong to respectively. And there will be peak overlap in the high-performance liquid chromatography data, resulting in information errors during the decomposition of IMF components, and it is impossible to directly judge the hemoglobin type based on the information in the hemoglobin component IMF components. The present invention further takes into account that the information corresponding to the same hemoglobin component in the high-performance liquid chromatography data at different temperatures is similar, and the degree of peak overlap is different. Therefore, through statistical methods, the common features of the hemoglobin component IMF components at all temperatures can be statistically analyzed to determine the hemoglobin type and content of each hemoglobin component IMF component. Therefore, in the embodiments of the present invention, it is necessary to perform matching analysis on each hemoglobin component IMF component, match the hemoglobin component IMF component with each hemoglobin component IMF component at different temperatures to obtain a temperature matching component set for each hemoglobin component IMF component. Each component in this temperature matching component set corresponds to a temperature. Considering that each component in the set should belong to the same hemoglobin type as the corresponding hemoglobin component IMF component, its peak feature distinctness and retention time in the chromatogram should be similar. Therefore, the basis for matching during the matching process should be to match according to the difference in peak feature distinctness and retention time between hemoglobin component IMF components.

[0055] Preferably, in an embodiment of the present invention, the matching process includes:

[0056] Optionally select a temperature as the target temperature, take one IMF component of a hemoglobin component at the target temperature as the target component, and sequentially take each IMF component of the hemoglobin component at other temperatures as the comparison components.

[0057] Obtain a matching index based on the difference in the distinctness of the peak features and the difference in the retention time between the target component and the comparison components; at one of the other temperatures, take the comparison component corresponding to the maximum matching index as the temperature matching component of the target component at the other temperature; obtain the temperature matching components of the target component at all other temperatures, and obtain the set of temperature matching components of the target component.

[0058] Furthermore, in the embodiments of the present invention, the method for obtaining the matching index includes:

[0059] Multiply the difference in the distinctness of the peak features by the difference in the retention time, perform a negative correlation mapping and normalization to obtain the matching index. That is, the smaller the difference in the two dimensions, the more the two components belong to the same type of hemoglobin, and the larger the matching index.

[0060] As an example, in an embodiment of the present invention, the matching index is expressed by the formula:

[0061] P(t1.j,t2.k)=exp(-(|F t1,j -F t2,k |×|T t1,j -T t2,k |));where P(t1.j,t2.k) is the matching index between the jth IMF component of the hemoglobin component at the t1th temperature and the kth IMF component of the hemoglobin component at the t2th other temperature. That is, the t1th temperature can be regarded as the target temperature, the jth IMF component of the hemoglobin component is the target component, and the kth IMF component of the hemoglobin component is the comparison component; F t1,j is the distinctness of the peak feature of the jth IMF component of the hemoglobin component at the t1th temperature; T t1,j is the retention time of the jth IMF component of the hemoglobin component at the t1th temperature; F t2,k is the distinctness of the peak feature of the kth IMF component of the hemoglobin component at the t2th other temperature; T t2,k is the retention time of the kth IMF component of the hemoglobin component at the t2th other temperature; exp() is the exponential function with the natural constant as the base. In this formula, the result is negatively correlated and normalized through the exponential function with the natural constant as the base.

[0062] It should be noted that the statistical method of the retention time is a well-known technical means to those skilled in the art and will not be elaborated here.

[0063] Step S4: For each IMF component of hemoglobin, obtain the hemoglobin type of each IMF component of hemoglobin according to the retention time of each component in the temperature-matched component set; count the peak areas of the IMF components of hemoglobin of each hemoglobin type at different temperatures to obtain the content of each hemoglobin type in the blood sample.

[0064] In the preprocessing process of the above high-performance liquid chromatography analysis method, the hemoglobin components are separated by a cation exchange column using the potential difference, and a variety of hemoglobins are separated by gradient using eluents with different concentrations, and can be separated into six hemoglobin components. That is, there are six hemoglobin types, and each hemoglobin type corresponds to a standard retention time under one temperature condition. Therefore, for each IMF component of hemoglobin, the hemoglobin type of each IMF component of hemoglobin can be obtained according to the retention time of each component in the temperature-matched component set. That is, by statistically analyzing the retention time characteristics of the matched components at all temperatures, the hemoglobin type of the IMF component of hemoglobin is comprehensively determined.

[0065] Preferably, in an embodiment of the present invention, the method for determining the hemoglobin type of each IMF component of hemoglobin includes:

[0066] Obtain the standard retention time of each hemoglobin type at each temperature.

[0067] Optionally select one hemoglobin type as the target type. For an IMF component of hemoglobin, obtain the retention time difference between the retention time of each component in the temperature-matched component set of the protein component IMF component and the standard retention time at the temperature corresponding to the target type, and take the average retention time difference as the type discrimination degree between the IMF component of hemoglobin and the target type. That is, the smaller the average retention time difference, the smaller the type discrimination degree between the IMF component of hemoglobin and the target type, and the more likely it belongs to the target type.

[0068] As an example, the type discrimination degree is expressed by the formula:

[0069] where β i,j is the type discrimination degree between the jth hemoglobin IMF component and the ith hemoglobin type, that is, the ith hemoglobin type can be regarded as the target type; W is the number of components in the temperature-matched component set of the jth hemoglobin IMF component; Tm j,t is the retention time of the tth component in the temperature-matched component set of the jth hemoglobin IMF component, and T0 i,j is the standard retention time of the ith hemoglobin type at the temperature corresponding to the jth hemoglobin IMF component.

[0070] Obtain the type discrimination degree between each IMF component of hemoglobin and each hemoglobin type, and obtain the hemoglobin type of each IMF component of hemoglobin according to the type discrimination degree.

[0071] Further, in the embodiments of the present invention, for each IMF amount of hemoglobin component, select the hemoglobin type with the smallest type discrimination degree as the hemoglobin type of the IMF amount of the hemoglobin component.

[0072] At each temperature, the corresponding hemoglobin type can be determined for each IMF component of hemoglobin, and then the content of each hemoglobin type can be determined according to the peak area of the component at each temperature. By statistically analyzing the content determined by the peak area at different temperatures, the error caused by temperature influence can be eliminated, and a more accurate hemoglobin content result can be obtained.

[0073] Preferably, in an embodiment of the present invention, obtaining the content of each hemoglobin type in a blood sample includes:

[0074] At each temperature, take the peak area of the target peak of the IMF component of hemoglobin of each hemoglobin type as the target area, and obtain the area ratio of the target area of each hemoglobin type among all target areas.

[0075] For each hemoglobin type, take the average value of the area ratios at all temperatures as the content of the hemoglobin type. That is, the final content can be regarded in the form of a percentage. For the content of glycated hemoglobin, the standard range is generally between 4% - 6%. By comparing the difference between the percentage of glycated hemoglobin content and the standard range, a certain evaluation of the current health status and diabetes control target of pregnant women with diabetes can be made, assisting doctors in making decision judgments.

[0076] In summary, the embodiments of the present invention obtain high - performance liquid chromatography diagrams at different temperatures, use EMD modal decomposition to process the chromatograms and screen according to peak characteristics to determine the IMF components of hemoglobin. Match the IMF components of hemoglobin between different temperatures, and obtain the hemoglobin type of each IMF component of hemoglobin according to the obtained temperature - matched components combined with the retention time of the medium components. Then, statistically analyze the content of each hemoglobin type at all temperatures to obtain the content of each hemoglobin type in the blood sample. The present invention eliminates the errors in detection through chromatographic detection and data statistics methods, and obtains a more accurate detection result of glycated hemoglobin in pregnant women with diabetes.

[0077] It should be noted that: the above order of the embodiments of the present invention is only for description and does not represent the superiority or inferiority of the embodiments. The processes depicted in the accompanying drawings do not necessarily require the particular order or sequential order shown to achieve the desired results. In certain embodiments, multitasking and parallel processing are also possible or may be advantageous.

[0078] Each embodiment in this specification is described in a progressive manner. For the same or similar parts among the embodiments, reference can be made to each other. The key point of each embodiment is to illustrate the differences from other embodiments.

Claims

1. A high performance liquid chromatography method for detecting glycosylated hemoglobin in pregnant women with diabetes, characterized in that: The method comprises: Obtaining a blood sample from a pregnant woman with diabetes, and obtaining a high performance liquid chromatogram of the blood sample at different temperatures after pretreatment; For each HPLC chromatogram, performing EMD decomposition on the HPLC chromatogram to obtain a plurality of IMF components, and screening the IMF components of the hemoglobin component according to the peak characteristic significance of the IMF components; For each hemoglobin component IMF component, the hemoglobin component IMF component is matched with each hemoglobin component IMF component at different temperatures to obtain a temperature matching component set for each hemoglobin component IMF component; each hemoglobin component IMF component in the temperature matching component set corresponds to a temperature; the matching is based on differences in peak feature significance and retention time between the hemoglobin component IMF components; For each hemoglobin component IMF component, the hemoglobin type of each hemoglobin component IMF component is obtained according to the retention time of each component in the temperature matching component set; the peak areas of the hemoglobin component IMF components of each hemoglobin type at different temperatures are counted to obtain the content of each hemoglobin type in the blood sample.

2. The high performance liquid chromatography method for detecting glycated hemoglobin in pregnant women with diabetes according to claim 1, characterized in that: The method for obtaining the peak feature significance includes: The peak with the largest peak point signal value in the IMF component is taken as the target peak, and the peak feature visibility is obtained according to the peak point signal value, wavelength range and peak symmetry of the target peak.

3. The high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes according to claim 1, characterized in that: The screening method of the hemoglobin component IMF component comprises: Among all the IMF components at one temperature, the IMF component whose peak characteristic significance is greater than a preset screening threshold is taken as the IMF component of the hemoglobin component.

4. The high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes according to claim 1, characterized in that: The matching process includes: A temperature is selected as a target temperature, an IMF component of a hemoglobin component at the target temperature is selected as a target component, and the IMF components of each hemoglobin component at other temperatures are selected as comparison components in turn; A matching index is obtained based on the difference in peak feature significance and retention time between the target component and the comparison component; at one other temperature, the comparison component corresponding to the maximum matching index is used as the temperature matching component of the target component at the other temperature; the temperature matching component of the target component at all other temperatures is obtained, and a set of temperature matching components of the target component is obtained.

5. The high performance liquid chromatography method for detecting glycated hemoglobin in pregnant women with diabetes according to claim 4, characterized in that: The method for obtaining the matching index includes: The peak feature difference is multiplied by the retention time difference, and then negative correlation mapping is performed and normalized to obtain the matching index.

6. The high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes according to claim 1, characterized in that: The method for determining the hemoglobin type of each hemoglobin component IMF component comprises: Obtain the standard retention time of each hemoglobin type at each temperature; Select a hemoglobin type as the target type, and for a hemoglobin component IMF component, obtain the retention time difference between the retention time of each component in the temperature matching component set of the protein component IMF component and the standard retention time at the corresponding temperature of the target type, and use the average retention time difference as the type distinction between the hemoglobin component IMF component and the target type; The type distinction between each hemoglobin component IMF component and each hemoglobin type is obtained, and the hemoglobin type of each hemoglobin component IMF component is obtained according to the type distinction.

7. The high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes according to claim 2, characterized in that: The step of obtaining the content of each type of hemoglobin in the blood sample comprises: At each temperature, the peak area of ​​the target peak of the IMF component of the hemoglobin component of each hemoglobin type is taken as the target area, and the area ratio of the target area of ​​each hemoglobin type in all target areas is obtained; For each hemoglobin type, the average of the area percentages at all temperatures was taken as the content of the hemoglobin type.

8. The high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes according to claim 1, characterized in that: The pretreatment process of the blood sample includes: The blood sample is centrifuged, the supernatant is taken, a deproteinizing agent is added to obtain a deproteinized sample, the sample is diluted, a C18 column is selected for separation, and 20% acetonitrile and 80% phosphate buffer are prepared.

9. The high performance liquid chromatography detection method for glycated hemoglobin in pregnant women with diabetes according to claim 2, characterized in that: The method for obtaining the wave crest symmetry comprises: The peak point of the target wave crest divides the target wave crest into two wave crest regions, and the wave crest symmetry is obtained according to the area difference between the two wave crest regions.

10. The high performance liquid chromatography method for detecting glycated hemoglobin in pregnant women with diabetes according to claim 6, characterized in that: For each hemoglobin component IMF amount, the hemoglobin type with the smallest type distinction is selected as the hemoglobin type of the hemoglobin component IMF amount.

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