Skin care product based on chitosan and glucan modification and preparation method thereof

Abstract

The invention discloses a skin care product based on chitosan and glucan modification and a preparation method thereof, and relates to the technical field of skin care products. The skin care product is prepared from the following raw materials in parts by mass: 0.08 to 0.12 part of biological polysaccharide and the balance of deionized water, the biological polysaccharide is prepared from modified chitosan and modified beta-glucan; the preparation method comprises the following steps: adding the biological polysaccharide into the deionized water, uniformly stirring, and adding water until the total part number is 100, so as to obtain the skin care product. The prepared modified chitosan and modified beta-glucan are added into the skin care product to serve as biological polysaccharides, a synergistic effect is achieved, and the skin care product has good skin repairing capacity, anti-inflammatory capacity and skin aging resisting effects.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, in particular to a chitosan- and dextran-modified skin care product and a preparation method thereof. Background Art

[0002] In recent years, as people pay more attention to skin health and beauty, the market demand for skin care products has continued to increase. Chitosan and dextran, as natural polysaccharides, are widely used in the fields of medicine and cosmetics due to their excellent biocompatibility and biodegradability. Studies have shown that chitosan has good antibacterial, moisturizing and wound healing properties; dextran is a polysaccharide composed of glucose units, which performs well in promoting cell proliferation and repair. Many relevant patents and literature show that the use of chitosan or dextran alone can improve certain functions of the skin, but there is still room for improvement.

[0003] At present, there are many skin care products containing chitosan or glucan on the market. The main function of chitosan is to lock in moisture by forming a protective film, and its antibacterial properties can effectively inhibit the growth of bacteria on the skin surface. Glucan helps improve the overall condition of the skin by promoting the regeneration and repair of skin cells. However, in the prior art, the use of chitosan and glucan alone often fails to give full play to their synergistic effect, resulting in limited effects in improving skin efficacy. Therefore, exploring the synergistic effect of modified chitosan and modified glucan has become a hot topic in current research.

[0004] Although chitosan and glucan each have significant skin improvement effects, in actual applications, single-ingredient products often cannot meet consumers' demands for multiple skin care effects. In addition, although existing modification technologies can improve the performance of chitosan and glucan, in actual applications, how to effectively combine these two ingredients to achieve a synergistic effect is still a technical problem that needs to be solved. Therefore, systematic research on the synergistic effect of modified chitosan and modified glucan can effectively combine the advantages of modified chitosan and modified glucan, especially in terms of skin barrier repair, anti-inflammatory ability enhancement and anti-skin aging, so as to maximize the satisfaction of consumers' diverse skin care needs.

[0005] In summary, in order to solve the above problems, it is of great significance to prepare a skin care product based on chitosan and β-glucan modification. Summary of the invention

[0006] The object of the present invention is to provide a skin care product based on modified chitosan and glucan and a preparation method thereof, so as to solve the problem raised in the prior art: how to modify chitosan and modified glucan to improve their synergistic effect and enhance the skin's repair ability, anti-inflammatory ability and anti-skin aging effect.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A chitosan- and glucan-modified skin care product comprises the following raw materials, based on 100 parts by mass: 0.08 to 0.12 parts of biopolysaccharide and the rest being deionized water; the biopolysaccharide comprises modified chitosan and modified β-glucan.

[0009] Preferably, the biopolysaccharide consists of 0.05-0.07 parts of modified chitosan and 0.03-0.05 parts of modified β-glucan, by weight.

[0010] Preferably, the preparation method of the modified chitosan comprises the following steps: (1) adding chitosan to a 1-1.5 wt % acetic acid solution and stirring the mixture to obtain a chitosan solution;

[0011] (2) adding L-arginine to 1.8-2 wt % morpholineethanesulfonic acid buffer and stirring evenly, adding N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in sequence, adjusting the pH to 6-6.5, and stirring for 2-3 h in an ice bath to obtain an activated arginine solution;

[0012] (3) Add the activated arginine solution prepared in step (2) dropwise into the chitosan solution prepared in step (1), stir evenly, stir at 20-25° C. for 12-16 h, transfer to a dialysis bag with a molecular weight cutoff of 4000-5000 D, dialyze with deionized water for 5-7 days, filter, and freeze-dry to obtain modified chitosan.

[0013] Preferably, the chitosan solution comprises the following raw materials in parts by weight: 0.8-1.2 parts of chitosan, 40-50 parts of 1-1.5wt% acetic acid solution; the activated arginine solution comprises the following raw materials in parts by weight: 2-2.2 parts of L-arginine, 30-40 parts of 1.8-2wt% morpholineethanesulfonic acid buffer, 1.3-1.4 parts of N-hydroxysuccinimide, and 3.4-3.5 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride.

[0014] Preferably, the molecular weight of the chitosan is 100-200 kD.

[0015] Preferably, the preparation method of the modified β-glucan comprises the following steps: adding β-glucan to a 0.3-0.5wt% 1-ethyl-3-methylimidazolium acetate aqueous solution and stirring evenly, stirring at 85-90°C for 3-4h, high-pressure microfluidization treatment, precipitation using anhydrous ethanol, re-dissolving with water, centrifugation, and freeze-drying the supernatant to obtain modified β-glucan.

[0016] More preferably, during the high-pressure microfluidization treatment, the pressure is 110-130 MPa and the time is 5-7 min.

[0017] More preferably, the modified β-glucan comprises the following raw materials in parts by mass: 0.8-1.2 parts of β-glucan, 90-100 parts of 0.3-0.5 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution.

[0018] More preferably, the molecular weight of the β-glucan is 200-400 kD.

[0019] More preferably, the preparation method of the skin care product comprises the following steps: adding the biological polysaccharide into deionized water, stirring evenly, and adding water to a total of 100 parts to obtain the skin care product.

[0020] Compared with the prior art, the beneficial effects of the present application are as follows:

[0021] The present invention prepares and studies the synergistic effect of modified chitosan and modified β-glucan in enhancing the skin repair ability, anti-inflammatory ability and anti-skin aging effect: the skin care product significantly improves the skin repair ability, can effectively promote skin wound healing, repair skin damage, and maintain skin barrier and stability. At the same time, the combination of the two shows a significant synergistic effect in anti-inflammatory, can effectively inhibit the expression of pro-inflammatory factors, and significantly up-regulate the expression of anti-inflammatory factors, with potential anti-inflammatory, sedative and stabilizing effects. In addition, the present invention also shows a good synergistic anti-skin aging effect by inhibiting the expression of MMP-2 and MMP-9.

[0022] The present invention uses L-arginine to modify the chitosan molecule, destroys the intramolecular hydrogen bond of the chitosan molecule, increases the solubility, enhances its dispersibility in the system, improves the film-forming effect, and thus enhances the skin care effect; and L-arginine can promote the generation of natural moisturizing factors, delay skin aging, and can synthesize NO through nitric oxide synthase, which can promote blood vessel synthesis and anti-inflammatory effect during wound healing; and the molecular weight of chitosan needs to be controlled at 100-200 kD. Too high molecular weight leads to high viscosity and difficult modification, while too low molecular weight has a general film-forming effect and poor skin care effect.

[0023] After dissolving β-glucan with 1-ethyl-3-methylimidazolium acetate aqueous solution, the modified β-glucan is obtained by high-pressure microfluidization treatment. 1-ethyl-3-methylimidazolium acetate helps to break the hydrogen bond network of β-glucan, increasing the solubility of β-glucan in water. Through high-pressure microfluidization treatment, the hydrogen bonds and van der Waals forces between glucan molecules are further broken, further enhancing its solubility and dispersibility, thus enhancing the skin care effect. However, when the pressure is too high, the structure of β-glucan is damaged and the skin care effect becomes poor. When the pressure is too low, the hydrogen bonds and van der Waals forces between molecules are less damaged. According to experiments, the optimal treatment is at 110-130 MPa for 5-7 minutes.

[0024] In addition, through the limitation of the substances and modification methods in the above formula, the modified skin care products in this application have produced significant technical effects: First, through the cell scratch experiment, it is verified whether the combined use of modified chitosan and modified β-glucan can effectively promote skin wound healing. The results show that the combination of the two has a significant synergistic effect in promoting skin wound healing. Second, RT-qPCR and ELISA experiments are used to clarify the synergistic effect of modified chitosan and modified β-glucan in inhibiting the expression of pro-inflammatory factors and up-regulating the expression of anti-inflammatory factors. The results show that the combination of the two can significantly enhance the anti-inflammatory effect of skin cells. Third, by detecting the expression of MMP-2 and MMP-9 by RT-qPCR, the results show that the combined use of modified chitosan and modified β-glucan can significantly inhibit the expression of these two matrix metalloproteinases at the transcriptional level, having potential anti-skin aging ability. Brief Description of the Drawings

[0025] Figure 1 It is a comparison diagram of cell healing in the skin barrier repair experiment in Test Experiment (1) of the present invention;

[0026] Figure 2 It is a comparison diagram of cell migration area in the skin barrier repair experiment in Test Experiment (1) of the present invention;

[0027] Figure 3 It is a data diagram of the transcriptional level expression of each factor in Anti-inflammatory Ability Detection Experiment A in Test Experiment (2) of the present invention;

[0028] Figure 4 It is a data diagram of the expression of each factor in Anti-inflammatory Ability Detection Experiment B in Test Experiment (3) of the present invention;

[0029] Figure 5 It is a data diagram of the transcriptional level expression of each matrix metalloproteinase in Anti-skin Aging Experiment in Test Experiment (4) of the present invention; Detailed Description of the Invention

[0030] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0031] It should be noted that there are no special restrictions on the purchasing manufacturers of all raw materials involved in the present invention. Exemplarily, they include: the molecular weight of chitosan is 100-200 kD; the molecular weight of β-glucan is 200-400 kD, sourced from Ganoderma lucidum; the CAS number of L-arginine: 74-79-3; the CAS number of morpholineethanesulfonic acid: 4432-31-9; the CAS number of N-hydroxysuccinimide: 6066-82-6; the CAS number of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride: 25952-53-8; the CAS number of 1-ethyl-3-methylimidazolium acetate: 143314-17-4.

[0032] In the following examples, "parts" refers to parts by mass, and the above-mentioned and other unmentioned raw materials are all commercially available.

[0033] Among them, the preparation method of the skin care products in each example and comparative example includes the following steps: Add 0.1 part of biological polysaccharide to 20 parts of deionized water and stir evenly, then add water to a total of 100 parts to obtain the skin care products.

[0034] Example 1: The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.05 part of modified chitosan, 0.05 part of modified β-glucan, and the rest is deionized water.

[0035] Among them, the preparation method of the modified chitosan includes the following steps: (1) Add 1 part of chitosan to 50 parts of 1.5 wt% acetic acid solution and stir evenly to obtain a chitosan solution;

[0036] (2) Add 2.1 parts of L-arginine to 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution and stir evenly. Then add 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in sequence, adjust the pH to 6, and stir for 2 h under ice bath conditions to obtain an activated arginine solution;

[0037] (3) Drop the activated arginine solution prepared in step (2) into the chitosan solution prepared in step (1), stir evenly, stir at 25 °C for 12 h, transfer it to a dialysis bag with a molecular weight cut-off of 5000 D, dialyze with deionized water for 6 days, filter, and freeze-dry to obtain modified chitosan.

[0038] Among them, the preparation method of the modified β-glucan includes the following steps: adding 1 part of β-glucan into 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution, stirring evenly, stirring at 85 °C for 3 h, performing high-pressure microfluidization treatment at a pressure of 120 MPa for 6 min, precipitating with absolute ethanol, redissolving with water, centrifuging, and freeze-drying the supernatant to obtain the modified β-glucan.

[0039] Example 2: The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.07 part of modified chitosan, 0.05 part of modified β-glucan, and the rest is deionized water.

[0040] Among them, the preparation method of the modified chitosan includes the following steps: (1) adding 1 part of chitosan into 50 parts of 1.5 wt% acetic acid solution, stirring evenly to obtain a chitosan solution;

[0041] (2) adding 2.1 parts of L-arginine into 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution, stirring evenly, sequentially adding 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, adjusting the pH to 6, and stirring for 2 h under ice bath conditions to obtain an activated arginine solution;

[0042] (3) dropping the activated arginine solution prepared in step (2) into the chitosan solution prepared in step (1), stirring evenly, stirring at 25 °C for 12 h, transferring to a dialysis bag with a cut-off molecular weight of 5000 D, dialyzing with deionized water for 6 days, filtering, and freeze-drying to obtain the modified chitosan.

[0043] Among them, the preparation method of the modified β-glucan includes the following steps: adding 1 part of β-glucan into 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution, stirring evenly, stirring at 85 °C for 3 h, performing high-pressure microfluidization treatment at a pressure of 120 MPa for 6 min, precipitating with absolute ethanol, redissolving with water, centrifuging, and freeze-drying the supernatant to obtain the modified β-glucan.

[0044] Example 3: The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.05 part of modified chitosan, 0.03 part of modified β-glucan, and the rest is deionized water.

[0045] Among them, the preparation method of the modified chitosan includes the following steps: (1) adding 1 part of chitosan into 50 parts of 1.5 wt% acetic acid solution, stirring evenly to obtain a chitosan solution;

[0046] (2) Add 2.1 parts of L-arginine to 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution and stir evenly. Then add 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in sequence, adjust the pH to 6, and stir for 2 h under ice bath conditions to obtain an activated arginine solution;

[0047] (3) Dropwise add the activated arginine solution prepared in step (2) into the chitosan solution prepared in step (1), stir evenly, stir at 25 °C for 12 h, transfer it to a dialysis bag with a molecular weight cut-off of 5000 D, dialyze with deionized water for 6 days, filter, and freeze-dry to obtain modified chitosan.

[0048] Among them, the preparation method of the modified β-glucan includes the following steps: Add 1 part of β-glucan to 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution and stir evenly. Stir at 85 °C for 3 h, perform high-pressure microfluidization treatment at a pressure of 120 MPa for 6 min, precipitate with absolute ethanol, redissolve with water, centrifuge, and freeze-dry the supernatant to obtain modified β-glucan.

[0049] Comparative Example 1: Based on Example 1, only add modified chitosan, specifically as follows:

[0050] The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.1 part of modified chitosan, and the rest is deionized water.

[0051] Among them, the preparation method of the modified chitosan includes the following steps: (1) Add 1 part of chitosan to 50 parts of 1.5 wt% acetic acid solution and stir evenly to obtain a chitosan solution;

[0052] (2) Add 2.1 parts of L-arginine to 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution and stir evenly. Then add 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in sequence, adjust the pH to 6, and stir for 2 h under ice bath conditions to obtain an activated arginine solution;

[0053] (3) Dropwise add the activated arginine solution prepared in step (2) into the chitosan solution prepared in step (1), stir evenly, stir at 25 °C for 12 h, transfer it to a dialysis bag with a molecular weight cut-off of 5000 D, dialyze with deionized water for 6 days, filter, and freeze-dry to obtain modified chitosan.

[0054] Comparative Example 2: Based on Example 1, only add modified β-glucan, specifically as follows:

[0055] The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.1 part of modified β-glucan, and the rest is deionized water.

[0056] Among them, the preparation method of the modified β-glucan includes the following steps: adding 1 part of β-glucan to 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution, stirring evenly, stirring at 85 °C for 3 h, subjecting to high-pressure microfluidization treatment at a pressure of 120 MPa for 6 min, precipitating with absolute ethanol, redissolving with water, centrifuging, and freeze-drying the supernatant to obtain the modified β-glucan.

[0057] Comparative Example 3, based on Example 1, the chitosan is not modified, and the rest of the process remains unchanged, specifically as follows:

[0058] The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.05 part of chitosan, 0.05 part of modified β-glucan, and the rest is deionized water.

[0059] Among them, the preparation method of the modified β-glucan includes the following steps: adding 1 part of β-glucan to 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution, stirring evenly, stirring at 85 °C for 3 h, subjecting to high-pressure microfluidization treatment at a pressure of 120 MPa for 6 min, precipitating with absolute ethanol, redissolving with water, centrifuging, and freeze-drying the supernatant to obtain the modified β-glucan.

[0060] Comparative Example 4, based on Example 1, chitosan with a molecular weight of 300 - 450 kD is selected, and the rest of the process remains unchanged, specifically as follows:

[0061] The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.05 part of modified chitosan, 0.05 part of modified β-glucan, and the rest is deionized water.

[0062] Among them, the preparation method of the modified chitosan includes the following steps: (1) adding 1 part of chitosan with a molecular weight of 300 - 450 kD to 50 parts of 1.5 wt% acetic acid solution, stirring evenly to obtain a chitosan solution;

[0063] (2) adding 2.1 parts of L-arginine to 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution, stirring evenly, sequentially adding 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, adjusting the pH to 6, and stirring for 2 h under ice bath conditions to obtain an activated arginine solution;

[0064] (3) dropping the activated arginine solution prepared in step (2) into the chitosan solution prepared in step (1), stirring evenly, stirring at 25 °C for 12 h, transferring to a dialysis bag with a cut-off molecular weight of 5000 D, dialyzing with deionized water for 6 days, filtering, and freeze-drying to obtain the modified chitosan.

[0065] Among them, the preparation method of the modified β-glucan includes the following steps: Add 1 part of β-glucan to 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution, stir evenly, stir at 85 °C for 3 h, perform high-pressure microfluidization treatment at a pressure of 120 MPa for 6 min, precipitate with anhydrous ethanol, redissolve with water, centrifuge, and freeze-dry the supernatant to obtain the modified β-glucan.

[0066] Comparative Example 5, based on Example 1, the β-glucan is not modified, and the rest of the process remains unchanged. Specifically as follows:

[0067] The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.05 part of modified chitosan, 0.05 part of β-glucan, and the rest is deionized water.

[0068] Among them, the preparation method of the modified chitosan includes the following steps: (1) Add 1 part of chitosan to 50 parts of 1.5 wt% acetic acid solution, stir evenly to obtain a chitosan solution;

[0069] (2) Add 2.1 parts of L-arginine to 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution, stir evenly, sequentially add 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, adjust the pH to 6, and stir for 2 h under ice bath conditions to obtain an activated arginine solution;

[0070] (3) Drop the activated arginine solution prepared in step (2) into the chitosan solution prepared in step (1), stir evenly, stir at 25 °C for 12 h, transfer to a dialysis bag with a molecular weight cut-off of 5000 D, dialyze with deionized water for 6 days, filter, and freeze-dry to obtain the modified chitosan.

[0071] Comparative Example 6, based on Example 1, increase the pressure of the high-pressure microfluidization treatment to 180 MPa, and the rest of the process remains unchanged. Specifically as follows:

[0072] The skin care product includes the following raw materials, calculated by 100 parts by mass: 0.05 part of modified chitosan, 0.05 part of modified β-glucan, and the rest is deionized water.

[0073] Among them, the preparation method of the modified chitosan includes the following steps: (1) Add 1 part of chitosan to 50 parts of 1.5 wt% acetic acid solution, stir evenly to obtain a chitosan solution;

[0074] (2) Add 2.1 parts of L-arginine to 40 parts of 1.9 wt% morpholineethanesulfonic acid buffer solution, stir evenly, sequentially add 1.35 parts of N-hydroxysuccinimide and 3.45 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, adjust the pH to 6, and stir for 2 h under ice bath conditions to obtain an activated arginine solution;

[0075] (3) The activated arginine solution prepared in step (2) is dropped into the chitosan solution prepared in step (1), stirred evenly, stirred at 25 °C for 12 h, transferred to a dialysis bag with a molecular weight cut-off of 5000 D, dialyzed with deionized water for 6 days, filtered, and freeze-dried to obtain modified chitosan.

[0076] Among them, the preparation method of the modified β-glucan includes the following steps: 1 part of β-glucan is added to 100 parts of 0.4 wt% 1-ethyl-3-methylimidazolium acetate aqueous solution, stirred evenly, stirred at 85 °C for 3 h, treated with high-pressure microfluidization at a pressure of 180 MPa for 6 min, precipitated with absolute ethanol, redissolved with water, centrifuged, and the supernatant is freeze-dried to obtain modified β-glucan.

[0077] Performance test: (1) Skin barrier repair experiment was carried out on Example 1 and Comparative Examples 1-2: Human skin keratinocytes HaCat were selected for adherent culture, and the culture conditions were 37 °C and 5% CO2; sterile Tips were used to scratch on the cell monolayer to form a skin injury model, and an equal amount of the prepared skin care product was added, and a blank group was set up. After 0, 6, 18, and 24 hours respectively, observe and record the cell healing situation, observe with a microscope and calculate the skin injury area, and compare the healing effects among groups; the experimental data are as Figure 1 、 2 shown; The same test was carried out on Comparative Examples 3-6, and the test data are shown in Table 1 in terms of the cell migration rate at 24 h.

[0078] (2) Anti-inflammatory ability detection experiment A was carried out on Example 1 and Comparative Examples 1-2: Human skin keratinocytes HaCat were selected for adherent culture, and the culture conditions were 37 °C and 5% CO2. An equal amount of the prepared skin care product was added, and a blank group was set up. After culturing for 24 hours, using RT-qPCR technology: extracting total cellular mRNA, performing reverse transcription, and then detecting the transcriptional level expressions of anti-inflammatory factors IL-4 and TGF-β, and pro-inflammatory factors TNF-α and IL-8 through RT-qPCR technology. The experimental data are as Figure 3 shown;

[0079] (3) Anti-inflammatory ability detection experiment B was carried out on Example 1 and Comparative Examples 1-2: Human skin keratinocytes HaCat were selected for adherent culture, and the culture conditions were 37 °C and 5% CO2. An equal amount of the prepared skin care product was added, and a blank group was set up. After culturing for 24 hours, the cell supernatant was collected, and using ELISA technology: using a human ELISA kit to detect the expressions of anti-inflammatory factors (IL-4, TGF-β, etc.) and pro-inflammatory factors (TNF-α, IL-8). The experimental data are as Figure 4 shown;

[0080] (4) Anti-skin aging experiment was conducted on Example 1 and Comparative Examples 1-2: Human skin keratinocytes HaCat were selected for adherent culture at 37°C and 5% CO2, and an equal amount of the prepared skin care products were added. A blank group was set up and cultured for 24 hours. Total cell mRNA was extracted using RT-qPCR technology, and the expression of matrix metalloproteinases MMP-2 and MMP-9 at the transcriptional level was detected by qPCR. The experimental data are shown in FIG. Figure 5 shown.

[0081] in, Figures 1-5 In the embodiment 1, β-glucan + chitosan is modified chitosan is comparative example 1; β-glucan is comparative example 2; and the control is a blank group.

[0082] Table 1

[0083] Item 24h Cell Migration Rate / % Example 1 64.61 Comparative Example 1 45.22 Comparative Example 2 39.84 Comparative Example 3 42.31 Comparative Example 4 51.37 Comparative Example 5 38.72 Comparative Example 6 44.56 Blank 20.04

[0084] Conclusion: Figure 1 , 2 It can be seen that the use of modified chitosan and modified β-glucan alone can significantly promote the wound healing of HaCat cells, and the effect of modified chitosan is better than that of modified β-glucan. When used in combination, the two show a significant synergistic effect. The healing effect can be observed in a short time (6 hours) and has a significant time dependence. It can effectively promote skin wound healing, repair skin damage, and maintain skin barrier and stability.

[0085] Depend on Figure 3 It can be seen that the use of modified chitosan and modified β-glucan alone can significantly upregulate the expression of anti-inflammatory factors IL-4 and TGF-β, and downregulate the expression of pro-inflammatory factors TNF-α and IL-8. When used in combination, the two show a significant synergistic effect and have potential anti-inflammatory, sedative and stabilizing effects.

[0086] Depend on Figure 4 It can be seen that the use of modified chitosan and modified β-glucan alone can significantly promote the expression of anti-inflammatory factors and downregulate the expression of pro-inflammatory factors. When the two are used in combination, they show significant synergistic effects and have potential anti-inflammatory, sedative and stabilizing effects.

[0087] Depend on Figure 5 It can be seen that the use of modified chitosan and modified β-glucan alone can significantly inhibit the expression of MMP-2 and MMP-9 at the transcriptional level in HaCat cells, and the combined use of the two shows a significant synergistic effect, which has potential synergistic anti-skin aging effects.

[0088] As can be seen from Table 1: In Comparative Example 3, chitosan was not modified, resulting in low water solubility, poor dispersibility, and reduced repair effect; in Comparative Example 4, chitosan with a molecular weight of 300 - 450 kD was selected, which had a high viscosity, was difficult to modify, had reduced dispersibility, and a reduced repair effect; in Comparative Example 5, β-glucan was not modified, resulting in low water solubility, poor dispersibility, and reduced repair effect; in Comparative Example 6, the pressure of high-pressure microfluidization treatment was increased to 180 MPa, and the structure of β-glucan was damaged, resulting in a decline in the repair effect.

[0089] In summary, by adding the prepared modified chitosan and modified β-glucan as biological polysaccharides to skin care products, the present invention has a good synergistic effect, with excellent skin repair ability, anti-inflammatory ability, and anti-skin aging effect.

[0090] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A skin care product based on chitosan or dextran modification, characterized in that: The skin care product comprises the following raw materials, based on 100 parts by mass: 0.08 to 0.12 parts of biopolysaccharide, and the rest is deionized water; the biopolysaccharide comprises modified chitosan and modified β-glucan.

2. The chitosan- and dextran-modified skin care product according to claim 1, characterized in that: In terms of weight, the biopolysaccharide consists of 0.05-0.07 parts of modified chitosan and 0.03-0.05 parts of modified beta-glucan.

3. The chitosan- and dextran-modified skin care product according to claim 1, characterized in that: The preparation method of the modified chitosan comprises the following steps: (1) adding chitosan to a 1-1.5 wt % acetic acid solution and stirring the mixture to obtain a chitosan solution; (2) adding L-arginine to 1.8-2 wt % morpholineethanesulfonic acid buffer and stirring evenly, adding N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride in sequence, adjusting the pH to 6-6.5, and stirring for 2-3 h in an ice bath to obtain an activated arginine solution; (3) Add the activated arginine solution prepared in step (2) dropwise into the chitosan solution prepared in step (1), stir evenly, stir at 20-25° C. for 12-16 h, transfer to a dialysis bag with a molecular weight cutoff of 4000-5000 D, dialyze with deionized water for 5-7 days, filter, and freeze-dry to obtain modified chitosan.

4. The chitosan- and dextran-modified skin care product according to claim 3, characterized in that: The chitosan solution comprises the following raw materials in parts by weight: 0.8-1.2 parts of chitosan and 40-50 parts of 1-1.5wt% acetic acid solution; the activated arginine solution comprises the following raw materials in parts by weight: 2-2.2 parts of L-arginine, 30-40 parts of 1.8-2wt% morpholineethanesulfonic acid buffer, 1.3-1.4 parts of N-hydroxysuccinimide, and 3.4-3.5 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride.

5. The chitosan- and dextran-modified skin care product according to claim 3, characterized in that: The molecular weight of the chitosan is 100-200 kD.

6. The chitosan- and dextran-modified skin care product according to claim 1, characterized in that: The preparation method of the modified β-glucan comprises the following steps: adding β-glucan to a 0.3-0.5wt% 1-ethyl-3-methylimidazole acetate aqueous solution and stirring evenly, stirring at 85-90°C for 3-4h, high-pressure microfluidization treatment, using anhydrous ethanol for precipitation, adding water for re-dissolution, centrifugation, and freeze-drying the supernatant to obtain the modified β-glucan.

7. The chitosan- and dextran-modified skin care product according to claim 6, characterized in that: During the high-pressure microfluidic treatment, the pressure is 110-130 MPa and the time is 5-7 minutes.

8. The chitosan- and dextran-modified skin care product according to claim 6, characterized in that: The modified beta-glucan comprises the following raw materials in parts by weight: 0.8 to 1.2 parts of beta-glucan and 90 to 100 parts of a 0.3 to 0.5 wt % 1-ethyl-3-methylimidazole acetate aqueous solution.

9. The chitosan- and dextran-modified skin care product according to claim 6, characterized in that: The molecular weight of the β-glucan is 200-400 kD.

10. A method for preparing a chitosan- or dextran-modified skin care product according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: adding biopolysaccharide into deionized water and stirring evenly, adding water to make the total amount of parts 100, and obtaining the skin care product.

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