Protein enzymolysis reaction system and process
By adopting dynamically adjustable homogeneous components in the proteolytic reaction system, the problem of agitation range immobilization and uneven distribution of shear force caused by the fixed stirring design is solved, and a more efficient proteolytic reaction is achieved.
Patent Information
- Application Number
- CN202510649240.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-20
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2045-05-20
AI Technical Summary
In the existing protein enzymatic reaction system, the fixed stirring design leads to the immobilization of the stirring range and the uneven distribution of shear force, and the inability to effectively treat high-viscosity protein solutions, resulting in a decrease in the enzymatic reaction rate.
Dynamically adjustable homogeneous components are adopted, including rotating main pipe, piston column and swing plate structure. By driving the lifting and rotation of the components, dynamic adjustment of the homogeneous components is achieved to ensure real-time adjustment of the stirring range and strength.
Through dynamically adjustable homogeneous components, the efficiency and homogeneity of protein enzymatic lysis reaction are improved, the limitations of fixed stirring design are solved, and the enzymatic lysis reaction rate and mass transfer efficiency are improved.
Smart Images

Figure CN120158366A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of protein enzymolysis, and in particular to a protein enzymolysis reaction system and process. Background Art
[0002] In a protein enzymolysis reaction system, the stirring mechanism is a core component affecting the homogeneity of material mixing and the enzymolysis efficiency. In the prior art, a fixed stirring design is generally adopted. For example, a rigid fan blade or paddle is driven by a motor to rotate unidirectionally (such as the rotating rod and fan blade structure described in the patent publication number CN217265779U). Although such a design can achieve the basic mixing function, it has significant limitations in practical applications: Fixed stirring range: The geometric shape and installation angle of traditional stirring components are fixed, resulting in their action area being limited to a certain fixed spatial level in the reactor. When processing a high-viscosity protein solution, local flow dead zones are easily formed, causing uneven contact between the substrate and enzyme molecules and resulting in a decrease in the enzymolysis reaction rate; Uneven shear force distribution: The shear force field generated by the fixed paddle is distributed in a gradient. The shear force in the area close to the stirring shaft may be too high, causing the free enzyme to denature and inactivate, while the shear force in the edge area is insufficient to fully disperse the aggregated substrate, affecting the mass transfer efficiency; During the enzymolysis reaction process, the substrate concentration, viscosity, and reaction progress continuously change, but the fixed stirrer cannot adjust the action range and intensity in real time. On the other hand, the fixed stirring mode causes part of the energy to be consumed in ineffective turbulence rather than effective mass transfer. Although in recent years, the immobilized enzyme reactor (IMER) technology has improved the enzyme stability through the carrier immobilization strategy, its supporting stirring system is still limited by the fixed structure and cannot solve the problems of diffusion resistance and local concentration gradient in the heterogeneous system. Therefore, developing a new stirring mechanism with a dynamically adjustable stirring range has become the key direction to break through the bottleneck of the existing enzymolysis efficiency. Summary of the Invention
[0003] The purpose of the present invention is to provide a protein enzymolysis reaction system and process to solve the problems raised in the above background art.
[0004] The technical solution of the present invention is: a protein enzymolysis reaction system, including an enzymolysis tank, a homogenization component is arranged inside the enzymolysis tank, and a driving component is arranged at the top of the enzymolysis tank. The driving component is used to drive the homogenization component to lift and rotate, and further includes; A piston column, the top end of the piston column is fixedly connected with a gantry top frame, and the gantry top frame is fixedly connected with the top of the enzymolysis tank; The homogenization component includes a rotating main pipe, which is movably connected to the top of the enzymolysis tank. The piston column is coaxially arranged with the rotating main pipe, and a plug column adapted to the inner diameter of the rotating main pipe is fixedly connected to the bottom end of the piston column. The plug column is movably connected to the inside of the rotating main pipe. A pair of swing plates are rotatably mounted on the outer peripheral wall of the rotating main pipe; A material distribution component, which is arranged on the inner wall of one end of the enzymolysis tank. A swing suction unit is arranged between the material distribution component and the rotating main pipe.
[0005] Preferably, the material distribution component includes an inner ring frame fixedly installed on the inner wall of one end of the enzymolysis tank. A rotating ring plate is rotatably mounted on the inner ring frame. The cross-section of the rotating ring plate is in a right trapezoidal structure. A plurality of arc-shaped grooves are formed on the surface of the rotating ring plate, and the plurality of arc-shaped grooves are distributed in an equidistant circular array.
[0006] Preferably, the swing suction unit includes a pair of communicating short pipes fixedly communicated with the rotating main pipe. A check valve four is fixedly installed on the communicating short pipe. The end of the communicating short pipe is rotatably communicated with a square pipe. The output direction of the check valve four is from the square pipe to the rotating main pipe. A sealing ring gasket is fixedly installed on the top of the rotating main pipe.
[0007] Preferably, a communicating cylinder is fixedly connected to the end of each square pipe. Filter through holes are evenly distributed on the communicating cylinder. Transfer seats are slidably mounted on the outer walls on both sides of each square pipe along its axial direction. Two pairs of inclined fixed pull rods are fixedly installed on the bottom outer wall of the rotating ring plate, and the end of the fixed pull rod is rotatably connected to the transfer seat.
[0008] Preferably, extension side rods are fixedly installed on the outer wall of one side of the two square pipes, and a transmission rod is fixedly installed at the end of the extension side rod. Long circular grooves are formed on the outer wall of one side of the two swing plates, and the transmission rod is movably matched with the long circular grooves. Turbulent flow grooves are evenly distributed on each swing plate.
[0009] Preferably, a pair of side pipes are fixedly communicated with the rotating main pipe. A check valve three is fixedly installed at the end of the side pipe close to the bottom. The output direction of the check valve three is from the bottom end of the side pipe to the rotating main pipe. Reinforcing plates are jointly fixedly installed on the outer wall of the two side pipes and the rotating main pipe. A pair of branch pipes are fixedly communicated with the rotating main pipe near the top end, and a check valve two is fixedly installed on each branch pipe. The output direction of the check valve two faces the rotating main pipe.
[0010] Preferably, a sector plate is fixedly connected to the bottom end of the rotating main pipe. A tree-shaped diversion groove communicated with the rotating main pipe is formed in the sector plate. A check valve one is fixedly installed at the end of the rotating main pipe close to the sector plate. The output direction of the check valve one is from the rotating main pipe to the tree-shaped diversion groove.
[0011] Preferably, the driving assembly includes a pair of electric push rods fixedly installed at the top of the enzymatic hydrolysis tank, and the ends of the extending rods of the two electric push rods are jointly fixedly installed with a mounting plate, and the mounting plate is rotatably connected to the rotating main pipe through a bearing. A supporting outer frame is fixedly installed on the outer peripheral wall of the enzymatic hydrolysis tank, and a feed pipe is fixedly communicated with one end of the outer peripheral wall of the enzymatic hydrolysis tank close to the top. The bottom end of the enzymatic hydrolysis tank is fixedly communicated with a discharge pipe with a valve.
[0012] Preferably, a driven bevel gear is fixedly installed on the outer peripheral wall of the rotating main pipe, a driving motor is fixedly installed on the mounting plate, and a driving bevel gear is fixedly installed on the output shaft of the driving motor. The driving bevel gear and the driven bevel gear are meshed with each other.
[0013] The present invention also discloses a protein enzymatic hydrolysis reaction process, which is applied to the protein enzymatic hydrolysis reaction system, and includes the following steps: S1. Prepare meat materials, select beef tenderloin or beef shank, and thoroughly remove the fascia. Cut the meat into small pieces of 3 cm 3 -5 cm 3 and then crush them into a paste and mix evenly. S2. Add the materials prepared in S1 into the enzymatic hydrolysis tank, select protease, dissolve the protease in warm water, and then add it into the enzymatic hydrolysis tank. S3. Use the homogenization assembly to fully mix the dissolved protease and the materials. S4. Use the enzymatic hydrolysis tank to carry out the enzymatic hydrolysis reaction of proteins. The enzymatic hydrolysis temperature is set at 40°C - 60°C, the substrate solid-liquid ratio is 1:5 to 1:10, the protease addition amount is 2% - 5%, and the enzymatic hydrolysis time is 2h - 4h.
[0014] By improving, the present invention provides a protein enzymatic hydrolysis reaction system and process herein. Compared with the prior art, it has the following improvements and advantages: First: When the rotating main pipe moves vertically under the action of the driving assembly in the present invention, the provided square pipe can use the end of the fixed pull rod as a leverage point, so that the square pipe swings along the connecting cylinder, thereby changing the angle of the connecting cylinder and making the material suction range wider. Second: When the square pipe swings along the connecting cylinder in the present invention, it can synchronously drive the extending side rod to swing, and under the combined action of the transmission rod and the long circular groove, synchronously adjust the elevation angle of the swing plate. Furthermore, when the rotating main pipe drives the swing plate to move up and down, the angle of the swing plate can change in real time, thereby ensuring the stirring effect on the materials. Thirdly: When the main pipe rotates downward and moves downward, since the piston column and the plug column are fixedly arranged, the space at the bottom of the piston column increases. Therefore, by using the negative pressure effect, the liquid in the enzymolysis tank is extracted through the filtering through holes on the connecting cylinder. At the same time, the space at the top of the piston column decreases, so the air above the piston column is pressed into the lower end of the rotating main pipe through the side pipe and the check valve III, further improving the cavitation effect on the material. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.
[0016] Figure 1 is the overall three-dimensional structure schematic diagram of the present invention; Figure 2 is of the present invention Figure 1 magnified structure schematic diagram at A in; Figure 3 is the partial sectional structure schematic diagram of the enzymolysis tank of the present invention; Figure 4 is the semi-sectional structure schematic diagram of the enzymolysis tank of the present invention; Figure 5 is the three-dimensional structure schematic diagram of the stirring assembly of the present invention; Figure 6 is of the present invention Figure 5 magnified structure schematic diagram at B in; Figure 7 is of the present invention Figure 5 magnified structure schematic diagram at C in; Figure 8 is the three-dimensional structure schematic diagram of the swing plate of the present invention; Figure 9 is the three-dimensional structure schematic diagram of the rotating ring plate of the present invention.
[0017] Reference numerals: 1. Enzymolysis tank; 101. Support outer frame; 102. Valve-equipped discharge pipe; 103. Feed pipe; 2. Electric push rod; 201. Mounting plate; 3. Gantry top frame; 301. Piston column; 302. Sealing ring gasket; 303. Plug column; 4. Rotating main pipe; 401. Driven conical gear; 402. Check valve I; 5. Driving motor; 501. Driving conical gear; 6. Inner ring frame; 601. Rotating ring plate; 602. Arc-shaped groove; 603. Fixed pull rod; 7. Side pipe; 701. Check valve III; 8. Branch pipe; 801. Check valve II; 9. Swing plate; 901. Oval slot; 902. Turbulent flow slot; 10. Square pipe; 11. Connecting cylinder; 111. Filter through hole; 12. Sector plate; 121. Dendritic diversion slot; 13. Connecting short pipe; 131. Check valve IV; 14. Extended side rod; 141. Transmission rod; 15. Stiffening plate; 16. Adapter seat. Detailed implementation mode
[0018] The present invention will be described in detail below. The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.
[0019] The present invention provides a protein enzymolysis reaction system and process by improvement. The technical solution of the present invention is as follows: As Figures 1 to 9 shown, the embodiment of the present invention provides a protein enzymolysis reaction system, including an enzymolysis tank 1. A homogenization component is arranged inside the enzymolysis tank 1, and a driving component is arranged on the top of the enzymolysis tank 1. The driving component is used to drive the homogenization component to lift and rotate. When the homogenization component moves vertically, the operation range can be increased, so as to achieve the purpose of improving the stirring effect. It also includes; A piston column 301, the top end of the piston column 301 is fixedly connected to a gantry top frame 3, and the gantry top frame 3 is fixedly connected to the top of the enzymolysis tank 1; The homogenization component includes a rotating main pipe 4. The rotating main pipe 4 is movably connected to the top of the enzymolysis tank 1. The piston column 301 is coaxially arranged with the rotating main pipe 4. The bottom end of the piston column 301 is fixedly connected to a plug column 303 adapted to the inner diameter of the rotating main pipe 4, and the plug column 303 is movably connected to the inside of the rotating main pipe 4. A pair of swing plates 9 are rotatably mounted on the outer peripheral wall of the rotating main pipe 4; A material distribution component, the material distribution component is arranged on the inner wall of one end of the enzymolysis tank 1, and a swinging suction unit is arranged between the material distribution component and the rotating main pipe 4; When the driving component controls the rotating main pipe 4 to move in the vertical direction, the swinging suction unit can be made to suck and discharge materials, so as to promote the full mixing of protease and materials.
[0020] Further, as Figures 2 - 5 shown, the material distributing assembly includes an inner ring frame 6 fixedly installed on the inner wall at one end of the enzymolysis tank 1, and a rotating ring plate 601 is rotatably installed on the inner ring frame 6. The cross-section of the rotating ring plate 601 is in a right trapezoidal structure, and a plurality of arc-shaped grooves 602 are formed on the surface of the rotating ring plate 601. The plurality of arc-shaped grooves 602 are distributed in an equidistant annular array; through the above structure, the materials added into the enzymolysis tank 1 will first fall on the rotating ring plate 601, and then be shunted through the plurality of arc-shaped grooves 602 provided. Finally, with the rotation of the rotating ring plate 601, the purpose of uniform feeding is achieved.
[0021] As a further solution of the present invention, the swinging material suction unit includes a pair of communicating short pipes 13 fixedly communicated with the rotating main pipe 4, and a check valve four 131 is fixedly installed on the communicating short pipe 13. The end of the communicating short pipe 13 is rotatably communicated with a square pipe 10. The output direction of the check valve four 131 is from the square pipe 10 to the rotating main pipe 4, and a sealing ring gasket 302 is fixedly installed on the top of the rotating main pipe 4.
[0022] Further, the end of each square pipe 10 is fixedly connected with a communicating cylinder 11, and uniformly distributed filtering through holes 111 are formed on the communicating cylinder 11. On the outer walls on both sides of each square pipe 10, adapter seats 16 are slidably installed along the axial direction. On the bottom outer wall of the rotating ring plate 601, two pairs of inclined fixed pull rods 603 are fixedly installed, and the end of the fixed pull rod 603 is rotatably connected with the adapter seat 16.
[0023] Through the above structure, when the rotating main pipe 4 rotates, it can drive the square pipe 10 to rotate. At the same time, with the cooperation of the provided adapter seats 16 and fixed pull rods 603, the rotating ring plate 601 can be driven to rotate, so as to realize the stirring operation and the material distribution during the feeding process; At the same time, when the rotating main pipe 4 moves vertically under the action of the driving assembly, the provided square pipe 10 can use the end of the fixed pull rod 603 as a leverage point, so that the square pipe 10 swings along the communicating cylinder 11, thereby changing the angle of the communicating cylinder 11 and making the material suction range wider.
[0024] As a further solution of the present invention, extension side rods 14 are fixedly installed on the outer walls on one side of the two square pipes 10, and a transmission rod 141 is fixedly installed at the end of the extension side rod 14. Long circular grooves 901 are formed on the outer walls on one side of the two swing plates 9, and the transmission rod 141 is movably matched with the long circular grooves 901. Uniformly distributed turbulent flow grooves 902 are formed on each swing plate 9.
[0025] Through the above structure, as Figures 5 - 8As shown in the figure, when the square pipe 10 swings along the connecting cylinder 11, it can drive the extension side rod 14 to swing synchronously. Under the combined action of the transmission rod 141 and the long circular groove 901, the elevation angle of the swing plate 9 is adjusted synchronously. Furthermore, while the rotating main pipe 4 drives the swing plate 9 to move up and down, the angle of the swing plate 9 can change in real time, thus ensuring the stirring effect on the material.
[0026] Furthermore, a pair of side pipes 7 are fixedly connected to the rotating main pipe 4. And a check valve three 701 is fixedly installed at one end of the side pipe 7 close to the bottom. The output direction of the check valve three 701 is from the bottom end of the side pipe 7 to the rotating main pipe 4. A stiffening plate 15 is fixedly installed jointly by the two side pipes 7 and the outer wall of the rotating main pipe 4. A pair of branch pipes 8 are fixedly connected to one end of the rotating main pipe 4 close to the top. And a check valve two 801 is fixedly installed on each branch pipe 8. And the output direction of the check valve two 801 faces the rotating main pipe 4. Through the above structure, when the rotating main pipe 4 moves downward, since the piston column 301 and the plug column 303 are fixedly arranged, therefore, the space at the bottom of the piston column 301 increases. Thus, by using the negative pressure effect, the liquid in the enzymatic hydrolysis tank 1 is extracted through the filtering through holes 111 on the connecting cylinder 11. At the same time, the space at the top of the piston column 301 decreases. Thus, the air above the piston column 301 is pressed into the lower end of the rotating main pipe 4 through the side pipe 7 and the check valve three 701. When the rotating main pipe 4 moves upward, the space at the bottom of the piston column 301 decreases. Thus, the inhaled liquid and air are transported to the subsequent sector plate 12 through the check valve one 402 and are ejected through the tree-shaped diversion groove 121. Thus, by using the cavitation effect, the purpose of improving the homogenization effect is achieved. In addition, the space at the top of the piston column 301 increases. And by using the negative pressure effect, air is re-extracted into the rotating main pipe 4 through the branch pipe 8 and the check valve two 801.
[0027] As a further solution of the present invention, as Figures 3 - 5 shown, the bottom end of the rotating main pipe 4 is fixedly connected to a sector plate 12. And a tree-shaped diversion groove 121 communicating with the rotating main pipe 4 is opened in the sector plate 12. A check valve one 402 is fixedly installed at one end of the rotating main pipe 4 close to the sector plate 12. And the output direction of the check valve one 402 is from the rotating main pipe 4 to the tree-shaped diversion groove 121. Through the above structure, when the rotating main pipe 4 rotates, it can drive the sector plate 12 to rotate, playing an auxiliary stirring role. And the provided tree-shaped diversion groove 121 can re-divert the air and the material, further improving the cavitation effect on the material.
[0028] As a further solution of the present invention, as Figure 1 、 Figure 2 and Figure 6As shown in the figure, the driving assembly includes a pair of electric push rods 2 fixedly installed at the top of the enzymolysis tank 1. The ends of the extension rods of the two electric push rods 2 are jointly fixedly installed with a mounting plate 201. The mounting plate 201 is rotatably connected to the rotating main pipe 4 through a bearing. A support outer frame 101 is fixedly installed on the outer peripheral wall of the enzymolysis tank 1. One end of the outer peripheral wall of the enzymolysis tank 1 near the top is fixedly communicated with a feed pipe 103. The bottom end of the enzymolysis tank 1 is fixedly communicated with a valve-equipped discharge pipe 102. After the protein enzymolysis is completed, the valve on the valve-equipped discharge pipe 102 can be opened for discharging operations.
[0029] Furthermore, a driven bevel gear 401 is fixedly installed on the outer peripheral wall of the rotating main pipe 4. A driving motor 5 is fixedly installed on the mounting plate 201. A driving bevel gear 501 is fixedly installed on the output shaft of the driving motor 5. The driving bevel gear 501 and the driven bevel gear 401 are meshed with each other.
[0030] With the above structure, by using the provided electric push rod 2, it can cooperate with the mounting plate 201 to drive the rotating main pipe 4 to move vertically. At the same time, when the driving motor 5 is controlled to start, through the transmission of the driving bevel gear 501 and the driven bevel gear 401, the rotating main pipe 4 can be driven to rotate for stirring operations.
[0031] The specific working method is as follows: During use, the driving motor 5 is controlled to start. Through the transmission of the driving bevel gear 501 and the driven bevel gear 401, the rotating main pipe 4 is driven to rotate. When the rotating main pipe 4 rotates, it can drive the square pipe 10 to rotate. At the same time, in cooperation with the provided adapter seat 16 and fixed pull rod 603, the rotating ring plate 601 can be driven to rotate. The materials added into the enzymolysis tank 1 will first fall on the rotating ring plate 601, and then be shunted through the provided multiple arc-shaped grooves 602. Finally, in cooperation with the rotation of the rotating ring plate 601, the purpose of uniform feeding can be achieved. When the rotating main pipe 4 moves vertically under the action of the driving assembly, the provided square pipe 10 can use the end of the fixed pull rod 603 as a leverage point, causing the square pipe 10 to swing along the connecting cylinder 11, thereby changing the angle of the connecting cylinder 11 and making the material suction range wider. When the square pipe 10 swings along the connecting cylinder 11, it can synchronously drive the extension side rod 14 to swing. And under the combined action of the transmission rod 141 and the long circular groove 901, the elevation angle of the swing plate 9 is synchronously adjusted. Furthermore, when the rotating main pipe 4 drives the swing plate 9 to move up and down, the angle of the swing plate 9 can change in real time, thus ensuring the stirring effect on the materials. When the rotating main pipe 4 moves downward, since the piston column 301 and the plug column 303 are fixedly arranged, the space at the bottom of the piston column 301 increases. Thus, by utilizing the negative pressure effect, the liquid in the enzymatic hydrolysis tank 1 is extracted through the filtering through holes 111 on the communicating cylinder 11. At the same time, the space at the top of the piston column 301 decreases, so that the air above the piston column 301 is pressed into the lower end of the rotating main pipe 4 through the side pipe 7 and the check valve three 701. When the rotating main pipe 4 moves upward, the space at the bottom of the piston column 301 decreases, so that the inhaled liquid and air are transported to the subsequent sector plate 12 through the check valve one 402 and bulge out through the tree-shaped diversion groove 121, thereby utilizing the cavitation effect to achieve the purpose of improving the homogenization effect. In addition, the space at the top of the piston column 301 increases, and by utilizing the negative pressure effect, air is re-extracted into the rotating main pipe 4 through the branch pipe 8 and the check valve two 801. The above operations can effectively homogenize the material. After the protein enzymatic hydrolysis is completed, the valve on the valve-equipped discharge pipe 102 can be opened for discharging operations.
[0032] Embodiment 2 This embodiment discloses a protein enzymatic hydrolysis reaction process, including the following steps: S1. Prepare meat materials, select beef tenderloin or beef shank, and thoroughly remove the fascia. Cut the meat into small pieces of 3 cm 3 -5 cm 3 and then grind them into a paste and mix well; S2. Add the materials prepared in S1 into the enzymatic hydrolysis tank 1, select protease, dissolve the protease in warm water, and then add it into the enzymatic hydrolysis tank 1; S3. Utilize the homogenization component to fully mix the dissolved protease with the materials; S4. Utilize the enzymatic hydrolysis tank 1 to carry out the enzymatic hydrolysis reaction of proteins. The enzymatic hydrolysis temperature is set at 40°C - 60°C, the substrate solid-liquid ratio is 1:5 to 1:10, the protease addition amount is 2% - 5%, and the enzymatic hydrolysis time is 2 h - 4 h.
[0033] The above description enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but rather to the broadest scope consistent with the principles and novel features disclosed herein.
Claims
1. A protein enzymatic hydrolysis reaction system, comprising an enzymatic hydrolysis tank (1), characterized in that: The enzymolysis tank (1) is provided with a homogenizing component inside, and a driving component is provided on the top of the enzymolysis tank (1), wherein the driving component is used to drive the homogenizing component to be lifted and rotated, and further comprises: A piston column (301), wherein the top end of the piston column (301) is fixedly connected to a gantry top frame (3), and the gantry top frame (3) is fixedly connected to the top of the enzymatic hydrolysis tank (1); The homogenizing assembly comprises a rotating main pipe (4), the rotating main pipe (4) being movably connected to the top of the enzymolysis tank (1), the piston column (301) being coaxially arranged with the rotating main pipe (4), and the bottom end of the piston column (301) being fixedly connected with a plug column (303) matching the inner diameter of the rotating main pipe (4), and the plug column (303) being movably connected to the inside of the rotating main pipe (4), and a pair of swing plates (9) being rotatably mounted on the outer peripheral wall of the rotating main pipe (4); A material distribution component is arranged on the inner wall of one end of the enzymolysis tank (1); a swinging material suction unit is arranged between the material distribution component and the rotating main pipe (4); the swinging material suction unit comprises a pair of connecting short pipes (13) fixedly connected to the rotating main pipe (4); a one-way valve (131) is fixedly installed on the connecting short pipe (13); the end of the connecting short pipe (13) is rotatably connected to the square pipe (10); the output direction of the one-way valve (131) is from the square pipe (10) to the rotating main pipe (4); and a sealing ring gasket (302) is fixedly installed on the top of the rotating main pipe (4).
2. A protein enzymatic hydrolysis reaction system according to claim 1, characterized in that: The material distribution assembly comprises an inner ring frame (6) fixedly mounted on the inner wall of one end of the enzymolysis tank (1), and a rotating ring plate (601) is rotatably mounted on the inner ring frame (6), the cross section of the rotating ring plate (601) is a right-angled trapezoidal structure, and a plurality of arc grooves (602) are formed on the surface of the rotating ring plate (601), and the plurality of arc grooves (602) are distributed in an equidistant annular array.
3. A protein enzymatic hydrolysis reaction system according to claim 2, characterized in that: The end of each square tube (10) is fixedly connected to a connecting tube (11), and the connecting tube (11) is provided with evenly distributed filtering holes (111). The outer walls on both sides of each square tube (10) are slidably mounted with an adapter seat (16) along its axial direction. The bottom outer wall of the rotating ring plate (601) is fixedly mounted with two pairs of inclined fixed rods (603), and the ends of the fixed rods (603) are rotatably connected to the adapter seat (16).
4. A protein enzymatic hydrolysis reaction system according to claim 3, characterized in that: An extended side rod (14) is fixedly mounted on one side outer wall of the two square tubes (10), and a transmission rod (141) is fixedly mounted on the end of the extended side rod (14). An elongated circular groove (901) is provided on one side outer wall of the two swing plates (9), and the transmission rod (141) is movably engaged with the elongated circular groove (901). Each swing plate (9) is provided with evenly distributed turbulent grooves (902).
5. A protein enzymatic hydrolysis reaction system according to claim 1, characterized in that: The rotating main pipe (4) is fixedly connected to a pair of side pipes (7), and a check valve (701) is fixedly installed on one end of the side pipe (7) close to the bottom, and the output direction of the check valve (701) is from the bottom end of the side pipe (7) to the rotating main pipe (4). A stiffening plate (15) is fixedly installed on the outer wall of the two side pipes (7) and the rotating main pipe (4). The rotating main pipe (4) is fixedly connected to a pair of branch pipes (8) on one end close to the top, and a check valve (801) is fixedly installed on each branch pipe (8), and the output direction of the check valve (801) is toward the rotating main pipe (4).
6. A protein enzymatic hydrolysis reaction system according to claim 1, characterized in that: The bottom end of the rotating main pipe (4) is fixedly connected to a fan-shaped plate (12), and a tree-shaped flow-dividing groove (121) communicating with the rotating main pipe (4) is provided in the fan-shaped plate (12). A one-way valve (402) is fixedly installed at one end of the rotating main pipe (4) close to the fan-shaped plate (12), and the output direction of the one-way valve (402) is from the rotating main pipe (4) to the tree-shaped flow-dividing groove (121).
7. A protein enzymatic hydrolysis reaction system according to claim 1, characterized in that: The driving assembly comprises a pair of electric push rods (2) fixedly mounted on the top of the enzymolysis tank (1), and the ends of the extension rods of the two electric push rods (2) are fixedly mounted with a mounting plate (201), and the mounting plate (201) is rotatably connected to the rotating main pipe (4) via a bearing, a supporting outer frame (101) is fixedly mounted on the outer peripheral wall of the enzymolysis tank (1), and one end of the outer peripheral wall of the enzymolysis tank (1) close to the top is fixedly connected to a feed pipe (103), and the bottom end of the enzymolysis tank (1) is fixedly connected to a discharge pipe (102) with a valve.
8. A protein enzymatic hydrolysis reaction system according to claim 7, characterized in that: A driven conical tooth (401) is fixedly mounted on the outer peripheral wall of the rotating main pipe (4), a driving motor (5) is fixedly mounted on the mounting plate (201), a driving conical tooth (501) is fixedly mounted on the output shaft of the driving motor (5), and the driving conical tooth (501) and the driven conical tooth (401) are meshed with each other.
9. A protein enzymatic hydrolysis reaction process, applied to the protein enzymatic hydrolysis reaction system according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Prepare meat materials. Choose beef tenderloin or beef leg. The fascia needs to be completely removed and the meat is cut into 3cm 3 -5cm 3 small pieces, and then mince them into a paste and mix them into a homogenous slurry; S2, add the material prepared in S1 to the enzymatic hydrolysis tank (1), and select a protease, add the protease to warm water to dissolve it, and then add it to the enzymatic hydrolysis tank (1); S3, use a homogenizing component to fully mix the dissolved protease with the material; S4, use the enzymatic hydrolysis tank (1) to perform an enzymatic hydrolysis reaction of the protein, the enzymatic hydrolysis temperature is set to 40°C-60°C, the substrate solid-liquid ratio is 1:5 to 1:10, the protease addition amount is 2%-5%, and the enzymatic hydrolysis time is 2h-4h.
Citation Information
Patent Citations
Enzymolysis reaction device for producing China-hemp polypeptide
CN217265779U
Dry anaerobic organic matter fermentation device
CN111454830A
Enzymolysis extraction device and process and application of enzymolysis extraction device and process in extraction of oriental cherry extract
CN116286333A
Production equipment and production method of bovine bone collagen peptide
CN116731855A
Hippophae rhamnoides seed protein extraction device
CN119220397A