Composite functional bacterial agent and application thereof in manure biogas residue and straw compost

Abstract

The invention relates to the technical field of manure biogas residue and straw compost, in particular to a composite functional microbial inoculum and application thereof in manure biogas residue and straw compost. The compound functional bacterial agent comprises strains including trichoderma longibrachiatum, thermoactinomyces daqu, bacillus licheniformis and pseudomonas aeruginosa; straw is introduced into the manure biogas residues, so that the carbon-nitrogen ratio of a compost mixture is precisely adjusted; according to the invention, a more suitable growth environment is created for microorganisms, the content of each strain in the microbial agent is accurately controlled, the synergistic effect of each strain is more efficient, the living environment of each strain in compost is improved, the activity of each strain is further enhanced, the decomposition capability of the composite functional microbial agent on complex organic matters in manure, biogas residues and straws is improved, and the resource utilization rate is increased. The method can be widely applied to the technical field of manure biogas residue and straw composting.

Description

Technical Field

[0001] The present invention relates to the technical field of manure biogas residue and straw composting, and specifically to a composite functional bacterium agent and its application in manure biogas residue and straw composting. Background Technique

[0002] With the development process of modern animal husbandry and agriculture, the output of livestock and poultry manure continues to increase, and at the same time, the quantity of crop straws is also extremely large. The annual resource output of livestock and poultry manure in China is about 4 billion tons, and the annual output of crop straws is even as high as hundreds of millions of tons. However, in terms of manure treatment, if directly using manure biogas residue for composting, due to its unreasonable carbon-nitrogen ratio, there are often many problems in the composting process, such as slow decomposition speed, poor compost quality, etc.

[0003] In order to optimize the composting effect of manure biogas residue, introducing crop straws becomes a key strategy. Straws are rich in carbon elements. Adding them to manure biogas residue can effectively adjust the carbon-nitrogen ratio of the composting materials, create more suitable conditions for the growth and metabolism of microorganisms, and thus significantly improve the performance of composting.

[0004] By reasonably mixing manure biogas residue and straws for composting and screening out a composite functional bacterium agent that can effectively promote their common decomposition, it is expected to significantly improve the composting efficiency, shorten the treatment time, reduce costs, and achieve the dual goals of efficient resource utilization and environmental protection. Summary of the Invention

[0005] The purpose of the present invention is to provide a composite functional bacterium agent and its application in manure biogas residue and straw composting to solve the problems raised in the above background technique.

[0006] To solve the above technical problems, the present invention provides the following technical solutions:

[0007] A composite functional bacterium agent, which contains strains of Trichoderma longibrachiatum, Thermoactinomyces daqus, Bacillus licheniformis, and Pseudomonas aeruginosa.

[0008] As a preference; the bacterial content of Trichoderma longibrachiatum in the composite functional bacterium agent is 10 5 -10 7 cfu / mL, the bacterial content of Thermoactinomyces daqus is 10 7 -10 9 cfu / mL, the bacterial content of Bacillus licheniformis is 10 7 -10 9cfu / mL, the bacterial content of Pseudomonas aeruginosa is 10 7 -10 9 cfu / mL.

[0009] A preparation method of a composite functional bacterial agent, which comprises the following preparation steps:

[0010] (1) Activate Trichoderma longibrachiatum in a malt extract agar solid medium, and then pick colonies and inoculate them into a liquid medium for cultivation to obtain a Trichoderma longibrachiatum bacterial liquid;

[0011] (2) Activate Thermoactinomyces vulgaris in a nutrient agar solid medium containing peptone and casein hydrolysate, and then pick colonies and inoculate them into a nutrient agar liquid medium containing peptone and casein hydrolysate for activation and cultivation to obtain Thermoactinomyces vulgaris bacterial liquid respectively;

[0012] (3) Activate Bacillus licheniformis in a nutrient agar solid medium containing peptone and beef extract, and then pick colonies and inoculate them into a nutrient agar liquid medium containing peptone and beef extract for activation and cultivation to obtain Bacillus licheniformis bacterial liquid;

[0013] (4) Activate Pseudomonas aeruginosa in a nutrient agar solid medium containing yeast extract and peptone, and then pick colonies and inoculate them into a liquid medium containing yeast extract and peptone for cultivation to obtain Pseudomonas aeruginosa bacterial liquid;

[0014] (5) Mix the Trichoderma longibrachiatum bacterial liquid, Thermoactinomyces vulgaris bacterial liquid, Bacillus licheniformis bacterial liquid and Pseudomonas aeruginosa bacterial liquid to obtain a microbial functional bacterial agent; the bacterial content of Trichoderma longibrachiatum in the functional bacterial agent is 10 5 -10 7 cfu / mL, the bacterial content of Thermoactinomyces vulgaris is 10 7 -10 9 cfu / mL, the bacterial content of Bacillus licheniformis is 10 7 -10 9 cfu / mL, the bacterial content of Pseudomonas aeruginosa is 10 7 -10 9 cfu / mL.

[0015] Preferably; the culture temperature of Trichoderma longibrachiatum is 28°C - 30°C, the culture temperature of Thermoactinomyces vulgaris is 50°C - 55°C, and the culture temperature of Bacillus licheniformis and Pseudomonas aeruginosa is 30°C - 35°C.

[0016] Preferably; before mixing the bacterial liquids, the viable count of the Trichoderma longibrachiatum bacterial liquid is not less than 2.0×10 7 cfu / mL, the viable counts of the Thermoactinomyces vulgaris bacterial liquid, Bacillus licheniformis bacterial liquid, and Pseudomonas aeruginosa bacterial liquid are not less than 2.0×109 cfu / mL.

[0017] Preferably, the main components and pH of the malt extract agar solid medium are as follows: malt extract 130.0 g, chloramphenicol 0.1 g, distilled water 1.0 L, pH 6.0, sterilized at 121 °C for 15 min;

[0018] The main components and pH of the nutrient agar solid medium containing peptone and casein hydrolysate are as follows: peptone 0.5 g, casein hydrolysate 0.5 g, yeast extract powder 0.5 g, soluble starch 0.5 g, dipotassium hydrogen phosphate 0.3 g, anhydrous magnesium sulfate 0.024 g, sodium pyruvate 0.3 g, glucose 0.5 g, distilled water 1.0 L, pH 7.0, sterilized at 121 °C for 15 min;

[0019] The main components and pH of the nutrient agar solid medium containing peptone and beef extract are as follows: beef extract 3.0 g, peptone 10.0 g, NaCl 5.0 g, distilled water 1.0 L, pH 7.0, sterilized at 121 °C for 15 min;

[0020] The main components and pH of the nutrient agar solid medium containing yeast extract and peptone are as follows: yeast extract 5.0 g, peptone 10.0 g, NaCl 10.0 g, distilled water 1.0 L, pH 7.0, sterilized at 121 °C for 15 min.

[0021] A method for treating fecal sewage biogas residue and straw compost using a composite functional bacterium agent, comprising the following steps:

[0022] (1) Mix the fecal sewage biogas residue and straw, and by controlling the ratio of the fecal sewage biogas residue and straw, make the carbon-nitrogen ratio of the compost mixture between 25 and 35.

[0023] (2) Add water to the compost and adjust the water content of the compost to 55%-65%,

[0024] (3) Then inoculate the composite functional bacterium agent in Claim 1 into the compost material at 0.5 mL / 100 g - 1.0 mL / 100 g, that is, inoculate 0.5 ml - 1.0 ml of the composite functional bacterium agent per 100 g of compost; aerobic fermentation for 35 days - 50 days.

[0025] Preferably; on the 3rd day, 7th day and 10th day of composting, perform turning pile treatment to increase the oxygen supply inside the compost.

[0026] The beneficial effects of the present invention compared with the prior art are as follows:

[0027] (1) The composite functional bacterium agent of the present invention significantly improves the efficiency of fecal sewage biogas residue and straw composting. Compared with the prior art, it shortens the time of aerobic fermentation and accelerates the composting and ripening process.

[0028] (2) By introducing straw into the manure sludge, the carbon-nitrogen ratio of the compost mixture was precisely adjusted to between 25 and 35, creating a more suitable growth environment for microorganisms, promoting the decomposition and maturation of organic matter, and improving the quality of the compost.

[0029] (3) The content of each bacterial species in the inoculant is precisely controlled, and the synergistic effect of each bacterial species is more efficient, thereby enhancing the ability to decompose complex organic matter in manure, sludge and straw, and improving resource utilization.

[0030] (4) By optimizing the culture medium composition and culture conditions, the activity and reproduction capacity of the bacterial strains are guaranteed, ensuring that the bacterial agent can achieve the best effect in treating manure sludge and straw compost.

[0031] (5) Turning the compost at specific times during the composting process helps maintain good aeration during the composting process, further promotes microbial activity and the decomposition of organic matter, and improves the uniformity and maturity of the compost.

[0032] Instruction Manual

[0033] Figure 1 The temperature change of composting under the treatment of composite functional bacterial agent in Example 2 of the present invention; wherein CK is a blank control group, and Mix is ​​a composite functional bacterial agent treatment group;

[0034] Figure 2 The change of germination index of composted Chinese cabbage seeds under the treatment of microbial agents in Example 2 of the present invention; wherein CK is a blank control group, and Mix is ​​a group treated with a composite functional bacterial agent;

[0035] Figure 3 The change of lignocellulose content in compost treated with microbial agents in Example 2 of the present invention; wherein CK is a blank control group, and Mix is ​​a composite functional agent treatment group;

[0036] Figure 4 The changes in compost enzyme activity under the treatment of microbial agents in Example 2 of the present invention; wherein CK is a blank control group, and Mix is ​​a group treated with a composite functional bacterial agent;

[0037] Figure 5 The effect of adding the composite functional bacterial agent and the comparative bacterial agent on the total nitrogen, total phosphorus and total potassium content of the compost in Example 3 of the present invention;

[0038] Figure 6 This is the effect of adding the composite functional bacterial agent and the comparative bacterial agent on the TOC content of the compost in Example 3 of the present invention. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0040] Example 1

[0041] The following are the specific preparation steps of the composite functional bacterial agent and related comparative bacterial agents:

[0042] 1. Material preparation: In the present invention, Trichoderma longibrachiatum (BNCC364475), Thermoactinomyces vulgaris (BNCC364387), Bacillus licheniformis (BNCC185404) and Pseudomonas aeruginosa (BNCC139675) are purchased from Hebei Beina Biotechnology Co., Ltd. Reagents such as beef extract, peptone, agar, and yeast powder are purchased from Beijing Solarbio Science & Technology Co., Ltd. Biogas residue and corn straw are taken from the experimental station of China Agricultural University.

[0043] 2. Bacterial strain activation:

[0044] a. Activation of Trichoderma longibrachiatum: The freeze-dried powder of Trichoderma longibrachiatum (BNCC364475) is fully dissolved with 0.5 mL of nutrient liquid medium (malt extract powder 130.0 g, chloramphenicol 0.1 g, distilled water 1.0 L, pH 6.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension is pipetted onto a solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate is placed in a constant temperature incubator and cultured at 30 °C, and the activation time is 72 h.

[0045] b. Activation of Thermoactinomyces vulgaris: For Thermoactinomyces vulgaris (BNCC364387), the freeze-dried powder is fully dissolved with 0.5 mL of nutrient liquid medium (peptone 0.5 g, casein hydrolysate 0.5 g, yeast extract powder 0.5 g, soluble starch 0.5 g, dipotassium hydrogen phosphate 0.3 g, anhydrous magnesium sulfate 0.024 g, sodium pyruvate 0.3 g, glucose 0.5 g, distilled water 1.0 L, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension is pipetted onto a solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate is placed in a constant temperature incubator and cultured at 55 °C, and the activation time is 24 h.

[0046] c. Activation of Bacillus licheniformis: The freeze-dried powder of Bacillus licheniformis (BNCC185404) was completely dissolved with 0.5 mL of nutrient liquid medium (3.0 g of beef extract, 10.0 g of peptone, 5.0 g of NaCl, 1.0 L of distilled water, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension was pipetted onto the solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate was placed in a constant temperature incubator and cultured at 35 °C for 24 h for activation.

[0047] d. Activation of Pseudomonas aeruginosa: The freeze-dried powder of Pseudomonas aeruginosa (BNCC139675) was completely dissolved with 0.5 mL of liquid medium (3.0 g of beef extract, 10.0 g of peptone, 5.0 g of NaCl, 1.0 L of distilled water, pH 7.0, sterilized at 121 °C for 15 min), and mixed evenly. Then, 0.5 mL of the bacterial suspension was pipetted onto the solid medium (adding 15.0 g of agar to the components of the liquid medium) plate and spread evenly with a spreader. The plate was placed in a constant temperature incubator and cultured at 35 °C for 24 h for activation.

[0048] 3. Freezing storage and activation use of strains:

[0049] Colonies were picked from the above plates. For each strain, 2 - 5 colonies were selected and washed into a 1.5 mL cryotube with an appropriate amount of 20% sterilized glycerol solution, sealed with a sealing film, and stored at -80 °C. Before each use, one tube of each strain was taken, melted at room temperature, and after melting, the bacterial solution was added to the corresponding liquid medium and mixed evenly, and then placed in a shaking incubator for enlarged culture at the corresponding temperature.

[0050] Preparation of the composite functional bacterium agent: The cultured Trichoderma longibrachiatum (T), Thermoactinomyces vulgaris TD, Bacillus licheniformis (BL), and Pseudomonas aeruginosa (PA) bacterial solutions were mixed evenly at a ratio of 1:1:1:1 to obtain the composite bacterium agent (Mix). The bacterial content of Trichoderma longibrachiatum in the composite bacterium agent was 10 6 cfu / mL, and the bacterial contents of Thermoactinomyces vulgaris TD, Bacillus licheniformis, and Pseudomonas aeruginosa were 10 8 cfu / mL.

[0051] Preparation of the control bacterium agent: The cultured Thermoactinomyces vulgaris TD, Bacillus licheniformis (BL), and Pseudomonas aeruginosa (PA) were respectively mixed with the Trichoderma longibrachiatum (T) bacterial solution at a volume ratio of 1:1 to obtain the bacterial solutions TTD, TBL, and TPA; the four bacterial solutions were mixed at a volume ratio of 1:1:1:1 to obtain the composite bacterium agent (Mix). The bacterial content of Trichoderma longibrachiatum in the composite bacterium agent was 10 6 cfu / mL, and the bacterial contents of Thermoactinomyces vulgaris TD, Bacillus licheniformis, and Pseudomonas aeruginosa were 108 cfu / mL.

[0052] Example 2

[0053] The following are the experimental steps and data of the manure biogas residue and straw compost added with the composite functional microbial agent and the blank control group:

[0054] 1. The experimental steps are as follows:

[0055] Mix the manure biogas residue and corn straw, adjust the initial carbon-nitrogen ratio of the material to 30, and adjust the initial moisture content of the material to 60%. Add 0.5 ml of the functional microbial agent (Mix) to every 100 g of the compost material and mix well. At the same time, set the treatment without adding the microbial agent as the control group (CK).

[0056] Carry out composting for 45 days, and control the fermentation temperature at room temperature. Turn the pile on the 3rd, 7th, and 15th days of composting to increase the oxygen supply in the pile body.

[0057] 2. The sampling time and sampling point requirements are as follows:

[0058] Samples are taken at the upper, middle, lower, left, and right points of the pile on the 0th, 5th, 10th, 15th, 25th, 35th, and 45th days of composting, mixed evenly, and stored at -20 °C for the determination of physical and chemical indexes.

[0059] 3. The sampling results are detected as follows:

[0060] (1) Changes in compost temperature

[0061] As Figure 1 shown, the highest temperatures of the control group CK and the treatment Mix with the microbial functional microbial agent are 59.2 °C and 64.5 °C respectively, and the number of days above 50 °C is 23 days and 27 days respectively. It can be seen that adding the microbial functional microbial agent increases the temperature during the high-temperature period of composting and extends the time of the high-temperature period. Temperature is an important index reflecting the metabolic intensity of microorganisms, indicating that adding the microbial agent can improve the activity of compost microorganisms, promote the degradation of organic matter, and improve the composting efficiency.

[0062] (2) Changes in the compost seed germination index

[0063] As Figure 2 shown, at the end of composting, the Chinese cabbage seed germination indexes of CK and Mix are 101.54% and 113.38% respectively. The results show that adding the microbial functional microbial agent can increase the seed germination index of compost and reduce its biological toxicity.

[0064] (3) Changes in the lignocellulose content of compost

[0065] From Figure 3It can be seen that at the end of composting, the degradation rates of cellulose, hemicellulose and lignin of CK and Mix were 33.11%, 60.13%, 27.48% and 53.25%, 64.66%, 38.14% respectively. Adding microbial functional agents can significantly improve the degradation rate of lignocellulose.

[0066] (4) Changes in enzyme activity during composting

[0067] Depend on Figure 4 It can be seen that during the entire composting process, the CMC enzyme activity, hemicellulase activity and laccase activity of Mix were significantly higher than those of CK treatment without the addition of bacterial agents. It can be seen that the addition of bacterial agents can increase the activity of microorganisms in the compost, improve the degradation of lignocellulose by increasing enzyme activity, and promote composting.

[0068] Example 3

[0069] The following is a comparison of the compost of manure, sludge and straw with the addition of composite functional bacteria and the compost of manure, sludge and straw with the addition of control bacteria:

[0070] Preparation of comparative microbial agents: The expanded Daqu thermoactinomycetes (TD), Bacillus licheniformis (BL), and Pseudomonas aeruginosa (PA) were mixed with the long-branch Trichoderma (T) bacterial solution in a volume ratio of 1:1 to obtain the microbial agents TTD, TBL, and TPA.

[0071] In addition, the Daqu thermoactinomycetes (TD), Bacillus licheniformis (BL), and Pseudomonas aeruginosa (PA) were mixed with the long-branch Trichoderma (T) bacterial liquid in a volume ratio of 1:1:1:1 to obtain a composite functional bacterial agent (Mix).

[0072] The content of Bifidobacterium longum in the above-mentioned bacterial agents is 10 6 cfu / mL, the bacterial content of acetic acid bacteria, cellulosic bacteria, and nitrogen-fixing bacteria is 10 8 cfu / mL.

[0073] Composting method: Mix the biogas residue and corn straw thoroughly and adjust the carbon-nitrogen ratio to 30, add water to a moisture content of 60%, inoculate 0.5 ml of bacterial solution for every 100 g of compost, mix well and place in a reactor, control the fermentation temperature at room temperature, and ferment aerobically for 45 days, turning the compost on the 3rd, 7th and 15th days of composting.

[0074] (1) Effects of inoculation with different bacterial solutions on total nitrogen, total phosphorus, and total potassium in compost

[0075] like Figure 5As shown in the figure, after 45 days of composting, except for CK, the total nitrogen, total phosphorus, and total potassium contents in the compost treated with the compound microbial agent (Mix) were higher than those treated with other microbial solutions. The order of nitrogen fixation ability from high to low was Mix>TBL>TTD>TPA>CK. The nitrogen fixation effect of treatment Mix was the best, and the total nitrogen loss decreased by 19.4% compared with CK. The total phosphorus and total potassium contents of treatment Mix reached 0.73% and 1.35% respectively, increasing by 40.38% and 20.53% respectively compared with CK.

[0076] (2) Effects of inoculating different microbial solutions on the total organic carbon (TOC) of compost

[0077] As Figure 6 shown in the figure, after 45 days of composting, the total organic carbon content in the compost treated with the compound microbial agent (Mix) was higher than that treated with other microbial solutions, increasing by 11.51% compared with the control group CK.

[0078] From the above results, it can be seen that adding the Mix functional microbial agent has the best effect on improving the nutrients of compost.

[0079] Experimental data analysis

[0080] Comparing all the above data, the following conclusions can be obtained:

[0081] (1) In Example 2, by comparing the compost treatment group with the compound functional microbial agent (Mix) and the blank control group (CK), the following conclusions were obtained. First, in terms of compost temperature, the highest temperature in the treatment group with Mix reached 64.5℃, and the number of days above 50℃ was significantly more than that of the control group. This indicates that the compound functional microbial agent can significantly enhance the activity of compost microorganisms, accelerate the degradation of organic matter, and improve the composting efficiency. Second, in terms of the seed germination index, the treatment group Mix was higher than CK, indicating that the microbial agent reduced the biological toxicity of the compost, improved the degree of maturity and safety. Third, the change in the lignocellulose content showed that the degradation rates of cellulose, hemicellulose, and lignin in the Mix treatment group were all higher than those of the control group, proving that the compound functional microbial agent has a significant promoting effect on the decomposition of lignocellulose. In addition, in terms of enzyme activity changes, the CMC enzyme activity, hemicellulase activity, and laccase enzyme activity in the Mix treatment group were all higher than CK, indicating that adding the microbial agent can enhance the microbial activity in the compost pile and accelerate the degradation and maturity of lignocellulose.

[0082] (2) Example 3 focused on comparing the composting effects of adding the compound functional microbial agent (Mix) with other comparative microbial agents. The Mix treatment group was superior to other comparative microbial agent treatment groups in terms of the total nitrogen, total phosphorus, and total potassium contents of the compost. In terms of nitrogen fixation ability, Mix performed the best, with a significant reduction in total nitrogen loss compared with the control group and a significant increase in total phosphorus and total potassium contents. In terms of the total organic carbon (TOC) content of the compost, the Mix treatment group was also higher than other comparative microbial agent treatment groups.

[0083] The reason why Mix has a better effect than the control inoculant lies in the synergistic effect among Trichoderma longibrachiatum, Thermoactinomyces vulgaris, Bacillus licheniformis and Pseudomonas aeruginosa. Each strain cooperates with each other to improve the living environment of the strains in the compost, thereby enhancing the activity of each strain in the compost, and jointly promoting the decomposition of organic matter and the transformation and retention of nutrients.

[0084] (3) Combining the results of Examples 2 and 3, the composite functional inoculant (Mix) performs excellently in the compost of fecal sewage biogas residues and straw. Whether it is the advantages of compost temperature, seed germination index, lignocellulose degradation and enzyme activity in Example 2, or the outstanding performance in nutrient content and total organic carbon content in Example 3, it shows that Mix is superior to the control group and other comparative inoculants. This fully confirms the effectiveness of the composite functional inoculant, and the synergistic effect among its strains plays a key role in promoting compost maturity, improving compost quality and nutrient value, opening up an effective way for the resource utilization of agricultural waste.

[0085] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and the descriptions in the specification are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of the present invention claimed is defined by the appended claims and their equivalents.

Claims

1. A composite functional bacterial agent, characterized in that: Contained are Trichoderma longibrachiatum, Thermoactinomyces daqus, Bacillus licheniformis and Pseudomonas aeruginosa.

2. A composite functional bacterial agent according to claim 1, characterized in that: The bacterial content of long-branched Trichoderma in the composite functional bacterial agent is 10 5 -10 7 cfu / mL, the bacterial content of Daqu thermophilic actinomycetes is 10 7 -10 9 cfu / mL, the bacterial content of Bacillus licheniformis is 10 7 -10 9 cfu / mL, the bacterial content of Pseudomonas aeruginosa was 10 7 -10 9 cfu / mL.

3. A method for preparing a composite functional bacterial agent, characterized in that: The method comprises the following preparation steps: (1) activating the long-branched trichoderma in a malt extract powder agar solid culture medium, then picking colonies and inoculating them in a liquid culture medium for cultivation to obtain a long-branched trichoderma bacterial liquid; (2) activating the Daqu thermophilic actinomycetes in a nutrient agar solid culture medium containing peptone and casein hydrolysate, respectively, and then picking colonies and inoculating them in a nutrient agar liquid culture medium containing peptone and casein hydrolysate for activation and cultivation, respectively, to obtain Daqu thermophilic actinomycete bacterial liquids; (3) activating Bacillus licheniformis in a nutrient agar solid medium containing peptone and beef extract, then picking colonies and inoculating them in a nutrient agar liquid medium containing peptone and beef extract for activation and cultivation to obtain a Bacillus licheniformis bacterial liquid; (4) activating Pseudomonas aeruginosa in a nutrient agar solid medium containing yeast extract and peptone, then picking colonies and inoculating them in a liquid medium containing yeast extract and peptone to obtain a Pseudomonas aeruginosa bacterial liquid; (5) Mixing a long-branched Trichoderma liquid, a Daqu thermophilic actinomycete liquid, a Bacillus licheniformis liquid and a Pseudomonas aeruginosa liquid to obtain a microbial functional bacterial agent; the long-branched Trichoderma content in the functional bacterial agent is 10 5 -10 7 cfu / mL, the bacterial content of Daqu thermophilic actinomycetes is 10 7 -10 9 cfu / mL, the bacterial content of Bacillus licheniformis is 10 7 -10 9 cfu / mL, the bacterial content of Pseudomonas aeruginosa was 10 7 -10 9 cfu / mL.

4. The method for preparing a composite functional bacterial agent according to claim 3, characterized in that: The culture temperature of long-branched wood enzyme bacteria is 28℃-30℃, the culture temperature of Daqu thermophilic actinomycetes is 50℃-55℃, and the culture temperature of Bacillus licheniformis and Pseudomonas aeruginosa is 30℃-35℃.

5. The method for preparing a composite functional bacterial agent according to claim 3, characterized in that: Before the bacterial solutions are mixed, the number of live bacteria in the long-branch Trichoderma solution is not less than 2.0×10 7 cfu / mL, the number of viable bacteria in the Daqu thermophilic actinomycete solution, the Bacillus licheniformis solution, and the Pseudomonas aeruginosa solution is not less than 2.0×10 9 cfu / mL.

6. The method for preparing a composite functional bacterial agent according to claim 3, characterized in that: The main components and pH of malt extract agar solid medium are as follows: malt extract 130.0g, chloramphenicol 0.1g, distilled water 1.0L, pH 6.0, sterilized at 121℃ for 15min; The main components and pH of the nutrient agar solid medium containing peptone and casein hydrolysate are as follows: white peptone 0.5g, casein hydrolysate 0.5g, yeast extract powder 0.5g, soluble starch 0.5g, dipotassium hydrogen phosphate 0.3g, anhydrous magnesium sulfate 0.024g, sodium pyruvate 0.3g, glucose 0.5g, distilled water 1.0L, pH 7.0, sterilized at 121℃ for 15min; The main components and pH of the nutrient agar solid medium containing peptone and beef extract are as follows: 3.0 g beef extract, 10.0 g peptone, 5.0 g NaCl, 1.0 L distilled water, pH 7.0, sterilized at 121°C for 15 min; The main components and pH of the nutrient agar solid culture medium containing yeast extract and peptone are as follows: 5.0 g yeast extract, 10.0 g peptone, 10.0 g NaCl, 1.0 L distilled water, pH 7.0, sterilized at 121°C for 15 min.

7. A method for treating manure, biogas residue and straw compost using a composite functional bacterial agent, characterized in that: The following steps are involved: (1) mixing the manure and digestate with the straw, and controlling the ratio of the manure and digestate to the straw so that the carbon-nitrogen ratio of the compost mixture is between 25 and 35; (2) Add water to the compost to adjust the moisture content of the compost to 55%-65%. (3) Then, the composite functional bacterial agent of claim 1 is inoculated into the compost material at a concentration of 0.5 mL / 100 g to 1.0 mL / 100 g, and aerobic fermentation is performed for 35 to 50 days.

8. A method for treating manure and biogas residue using a composite functional bacterial agent according to claim 7, characterized in that: On the 3rd, 7th and 10th days of composting, turn the compost to increase the oxygen supply inside the compost.

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