A strain of bacteria DY-10 for degrading phenanthrene and benzo(a)pyrene in oil-contaminated soil and its bacterial agent and application

Abstract

The application discloses a strain of bacteria DY-10 for degrading phenanthrene and benzopyrene in oil-contaminated soil, a bacterial agent thereof and application. The strain is Ochrobactrum teleogrylli DY-10, and the preservation number is GDMCC No:65988. Experiments prove that the strain DY-10 can utilize phenanthrene and benzopyrene as carbon sources to degrade them, and the degradation rates of the phenanthrene and the benzopyrene can both reach more than 70% after the strain is cultured in inorganic salt culture solution with the initial concentration of the phenanthrene or the benzopyrene being 50 mg·L ‑1 and 25 mg·L ‑1 respectively for 7 days. Therefore, the strain DY-10 is a strain capable of degrading phenanthrene and benzopyrene and having strong tolerance to the phenanthrene and the benzopyrene, has strong adaptability to polycyclic aromatic hydrocarbons, and has good application potential in biological remediation.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial technology, and particularly relates to a phenanthrene and benzopyrene degrading bacterium DY-10 in oil-contaminated soil, a bacterial agent thereof and application. BACKGROUND

[0002] With the rapid development of modern industrial processes, industrial pollution invades the soil more and more seriously. There are various persistent organic pollutants (POPs) in contaminated soil, such as polycyclic aromatic hydrocarbons (PAHs). PAHs are ubiquitous in the environment and accumulate, which has attracted widespread attention. Due to human activities such as industry, mining, agriculture and high soil environmental background value and other factors, the environment has been seriously polluted and seriously exceeded by polycyclic aromatic hydrocarbons. The main pollutants in chemical industry parks and surrounding soil, oil extraction areas, mining areas and sewage irrigation areas are caused by polycyclic aromatic hydrocarbons. Phenanthrene is a three-ring aromatic hydrocarbon, which has a very close relationship with the carcinogenicity of PAHs. With its unique chemical structure, phenanthrene has become a model compound for PAHs research. Benzopyrene is a planar polycyclic structure formed by the fusion of five benzene rings, and is a strong carcinogen. It can combine with DNA in human cells to form adducts and induce gene mutations. Due to the potential carcinogenic, teratogenic and mutagenic properties and biological accumulation of these two substances, they can pose a significant threat to the ecological environment and human health.

[0003] The natural attenuation of toxic and harmful organic pollutants in the environment mainly depends on the metabolic action of related microorganisms. The bioremediation technology has the advantages of low cost, good effect and no secondary pollution, so this method is the most potential remediation method for phenanthrene and benzopyrene pollution repair. At present, there are few reported phenanthrene and benzopyrene degrading strains, mainly including Virgibacillus, Chryseobacterium and Bacillus, etc. Because most of the microorganisms in the environment are not cultivable, many microorganisms, especially those with specific functions, cannot be isolated by pure culture. Therefore, it is of important application value and practical significance to screen strains that can effectively degrade high-concentration phenanthrene and benzopyrene.

[0004] The strain reported in the present study is Ochrobactrum teleogrylli, which can be isolated from various terrestrial and aquatic habitats. At present, there are few reports on the degradation of pollutants by Ochrobactrum teleogrylli. There is no report on the degradation of phenanthrene and benzopyrene by Ochrobactrum teleogrylli at home and abroad. SUMMARY

[0005] The first object of the present application is to provide a strain of Ochrobactrum teleogrylli DY-10, with the preservation number of GDMCC No: 65988.

[0006] The present application is obtained from domestication and isolation of a strain DY-10 (Ochrobactrum teleogrylli DY-10) using high-concentration phenanthrene and benzopyrene as carbon sources from a petroleum-contaminated soil in Ningbo, Zhejiang Province, and the strain is identified, and its growth characteristics and degradation characteristics of phenanthrene and benzopyrene are studied, thereby providing a reference for bioremediation of PAHs-contaminated environment.

[0007] The second object of the present application is to provide application of the Ochrobactrum teleogrylli DY-10 in degrading polycyclic aromatic hydrocarbons.

[0008] Preferably, the polycyclic aromatic hydrocarbons include phenanthrene and benzopyrene.

[0009] Preferably, the bacterial suspension of the Ochrobactrum teleogrylli DY-10 is applied to a polycyclic aromatic hydrocarbon-contaminated water body or soil.

[0010] The third object of the present application is to provide application of the Ochrobactrum teleogrylli DY-10 in preparing a polycyclic aromatic hydrocarbon-degrading bacterial agent.

[0011] The fourth object of the present application is to provide a polycyclic aromatic hydrocarbon-degrading bacterial agent, which comprises the Ochrobactrum teleogrylli DY-10 or a pure culture thereof as an active ingredient.

[0012] The fifth object of the present application is to provide application of the Ochrobactrum teleogrylli DY-10 or the bacterial agent in bioremediation of a polycyclic aromatic hydrocarbon-contaminated environment.

[0013] Preferably, the polycyclic aromatic hydrocarbon-contaminated environment includes a polycyclic aromatic hydrocarbon-contaminated water body and / or soil, and the polycyclic aromatic hydrocarbons include phenanthrene and benzopyrene.

[0014] The sixth object of the present application is to provide a method for degrading polycyclic aromatic hydrocarbons, which comprises applying the Ochrobactrum teleogrylli DY-10 or the bacterial agent to a polycyclic aromatic hydrocarbon-contaminated environment to degrade the polycyclic aromatic hydrocarbons.

[0015] This invention describes the domestication and isolation of a biodegrading strain, DY-10, from petroleum-contaminated soil in Ningbo City, Zhejiang Province, which utilizes phenanthrene and benzo[a]pyrene as carbon sources. Based on morphological analysis, 16S rDNA sequencing, and phylogenetic analysis, the strain was identified as *Ochrobactrum teleogrylli* DY-10. The optimal growth conditions for DY-10 were: temperature 28℃, pH 7, and sodium chloride content 1%. 16S rDNA sequencing analysis showed that the strain most closely related to DY-10 was *Ochrobactrum teleogrylli* strain JSTR40 (99.78%). DY-10 can utilize phenanthrene and benzo[a]pyrene as carbon sources, with initial concentrations of phenanthrene and benzo[a]pyrene of 50 mg·L⁻¹. -1 and 25 mg·L -1 After culturing in inorganic salt broth for 7 days, the degradation rate of these strains reached over 70%. Therefore, this strain has good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

[0016] Ochrobactrum teleogrylli DY-10 was deposited on February 26, 2025, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, 510070, China, with accession number GDMCC No:65988. Attached Figure Description

[0017] Figure 1 The images show the front and back views of strain DY-10 after 12 hours of growth on LB solid medium, as well as scanning electron microscope images of DY-10.

[0018] Figure 2 Phylogenetic information for strain DY-10.

[0019] Figure 3 The growth of strain DY-10 under different conditions.

[0020] Figure 4 The degradation efficiency of strain DY-10 in inorganic salt medium containing high concentrations of phenanthrene and benzo[a]pyrene (initial concentrations of phenanthrene and benzo[a]pyrene were 50 mg·L⁻¹) was calculated. -1 and 25 mg·L -1 ). Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0022] Example 1: Isolation and identification of Ochrobactrum teleogrylli DY-10

[0023] 1. Sample source

[0024] Soil samples were collected from a petroleum-contaminated soil in Ningbo City, Zhejiang Province, and long-term acclimation was carried out using high-concentration phenanthrene and benzopyrene as carbon sources. Efficient phenanthrene and benzopyrene-degrading bacteria were obtained through multiple screening and purification.

[0025] 2. Culture medium

[0026] 2.1 Inorganic salt medium

[0027] The inorganic salt medium was used for enrichment culture of microorganisms in the sample and phenanthrene and benzopyrene degradation experiments under pure bacterial conditions. The medium formula is shown in Table 1. The preparation method is to add each component to water, stir to mix, and sterilize to obtain.

[0028] Table 1 Formula of inorganic salt medium

[0029]

[0030]

[0031] 2.2 Nutrient medium

[0032] The nutrient medium was used for the isolation, purification, preservation, and activation of bacteria and the culture of conventional microorganisms. The types and components of the liquid nutrient medium used in this experiment are shown in Table 2. If solid medium is needed for the experiment, only 1.5-2% agar powder needs to be added to the original medium formula. If there is no special instruction for the culture conditions of the strain, the pH of the medium is adjusted to 7. The preparation method is to add each component to water, stir to mix, and sterilize to obtain.

[0033] Table 2 Composition of Luria-Bertani medium (LB)

[0034]

[0035] 3. Acclimation, screening, and isolation of strains

[0036] The collected contaminated soil was added to the enrichment medium (inorganic salt medium), and 50 mg·L -1 of phenanthrene and 25 mg·L -1 of benzopyrene were used as degradation substrates and placed in a 28°C incubator for culture without light. The inorganic salt medium with phenanthrene or benzopyrene as carbon source was used for strain acclimation, and 7 days was one acclimation cycle. A 10% inoculum was transferred to fresh enrichment medium with the same culture system and the above enrichment process was repeated, and this was repeated three times.

[0037] The fourth generation of the enriched culture sample obtained above was plated by dilution plating method, and the sample was separated with nutrient medium. The plated sample was incubated at the original culture temperature, and after about 48 hours, obvious single colonies were formed on the surface of the culture medium. Several single colonies with different characteristics were picked according to the size, color, transparency and other characteristics of the colonies, and were streaked and purified on a nutrient medium plate for culture. If different characteristic single colonies were still observed on the streaked and purified plate, the streaking separation was performed again until only single colonies with the same characteristics were observed on the same plate. In the experiment, one strain DY-10 with high-efficiency degradation performance for phenanthrene and benzopyrene was screened. The purified single colony was picked and cultured in the corresponding liquid nutrient medium to the logarithmic phase, and the bacterial liquid was mixed with sterile glycerol to be stored in sterile 2 mL cryotubes (glycerol concentration was 15%) at -80°C for long-term storage.

[0038] 4. Strain identification

[0039] 4.1 Morphological characteristics

[0040] DY-10 is a bacterium isolated from a petroleum-contaminated soil in Ningbo City, Zhejiang Province. After activation, the strain can form white, round, smooth-surfaced, slightly convex, opaque, non-spore, non-flagellum, short rod-shaped colonies with a diameter of 1.0-2.5 mm on a plate prepared from LB medium under aerobic conditions at 28°C for 12 hours. The transmission electron microscope image of the cell is shown in Figure 1 .

[0041] 4.2 Molecular biological characteristics

[0042] Molecular biological characteristics identification mainly includes sequencing and construction of phylogenetic tree. Before sequencing and construction of phylogenetic tree, the DNA of the bacterium needs to be extracted (the bacterial genomic DNA rapid extraction kit used in the experiment is from Beijing Aidley Biological Technology Co., Ltd.). In order to study the taxonomy of the bacterium, it is usually necessary to amplify the 16S rRNA gene and construct a phylogenetic tree. The amplified gene is a segment of DNA in the part of rRNA encoding in prokaryotes, which is usually used for detection and identification of bacteria due to its high conservation, specificity and suitable sequence length.

[0043] Polymerase chain reaction (PCR) is mainly used to amplify different gene fragments. Different primers (27F and 1492R; Baker et al., 2003) are needed for PCR. The PCR amplification reaction system is as follows: 10×buffer 2.5 μL, Mg 2+(25mmol / L) 1.5 μL, dNTP (25 mmol / L) 0.3 μL, forward primer (10 mmol / L) 0.5 μL, reverse primer (10 mmol / L) 0.5 μL, Taq enzyme: 0.25 μL, DNA group template 0.1 μL, deionized water 19.35 μL. PCR amplification reaction conditions: denaturation at 95°C, annealing at 55°C, extension at 72°C, cycle 30 times, extension at 72°C for 10 min, after the PCR reaction, store at 4°C. After amplifying the required gene, 0.75-1% agarose is added to the gel block, and nucleic acid staining agent GelRed is prepared into a gel block, PCR products and DNA markers containing various length fragments are added to the gel block and placed in an electrophoresis instrument, TBE (Tris borate) buffer is loaded into the electrophoresis instrument, and the electrophoresis instrument is operated at a certain voltage for 20 min, then taken out and placed under a 300 nm ultraviolet lamp to observe to determine whether the PCR product amplification reaction is successful. Then the amplified PCR product is sent to Huada Gene Technology Co., Ltd. for sequencing, and the sequencing primer is the same as the amplification primer.

[0044] The bacterial 16s rRNA gene sequence obtained by sequencing is uploaded to EzTaxon-e

[0045] (http: / / eztaxon-e.ezbiocloud.net / ), which compares the submitted sequence with the 16S rRNA gene sequence of the typical strain of the recognized species, and obtains the similarity information between the sequences. According to the analysis of the sequence alignment result, the corresponding typical strain can be selected as the model strain of the isolated strain in this experiment, and the 16S rRNA gene sequence of the model strain can also be obtained, and a phylogenetic analysis is constructed to prove that the model strain and the isolated strain have differences, so as to identify the isolated strain. The phylogenetic tree is constructed by using MEGA 5.05 program, and the neighbor-joining method, minimum evolution method and maximum parsimony method are usually used to construct the phylogenetic tree, among which the most commonly used is the neighbor-joining method, and the self-exhibition value is usually set to repeat 1000 times.

[0046] A 16S rRNA gene sequence with a length of 1359 bp is obtained by PCR and gene sequencing. Through 16S rRNA gene alignment, it is found that the strain has a gene similarity of 99.78% with Ochrobactrum teleogrylli strain JSTR40. From the above results, it can be concluded that the isolated bacteria DY-10 of the application is Ochrobactrum teleogrylli, and Ochrobactrum teleogrylli strain JSTR40 has a GenBank accession number of OM319748.

[0047] The 16S rRNA gene sequence of strain DY-10 is:

[0048] GGTCGCCTGCCTCCTTGCGGTTAGCACAGCGCCTTCGGGTAAAACCAACTCCCATGGT

[0049] GTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATTCTGATCCGCG

[0050] ATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGAT

[0051] GGCTTTTGGAGATTAGCTCACACTCGCGTGCTCGCTGCCCACTGTCACCACCATTGTA

[0052] GCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCC

[0053] TCTCGGCTTATCACCGGCAGTCCCCTTAGAGTGCCCAACTCAATGCTGGCAACTAAGG

[0054] GCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGA

[0055] CAGCCATGCAGCACCTGTATCCGGTCCAGCCGAACTGAAAGACACATCTCTGTGTCCG

[0056] CGACCGGTATGTCAAGGGCTGGTAAGGTTCTGCGCGTTGCTTCGAATTAAACCACATG

[0057] CTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACT

[0058] CCCCAGGCGGAATGTTTAATGCGTTAGCTGCGCCACCGAAGAGTAAACTCCCCGACGG

[0059] CTAACATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCAC

[0060] GCTTTCGCACCTCAGCGTCAGTAATGGACCAGTGAGCCGCCTTCGCCACTGGTGTTCC

[0061] TCCGAATATCTACGAATTTCACCTCTACACTCGGAATTCCACTCACCTCTTCCATACTCA

[0062] AGACTTCCAGTATCAAAGGCAGTTCCGGGGTTGAGCCCCGGGATTTCACCCCTGACTT

[0063] AAAAGTCCGCCTACGTGCGCTTTACGCCCAGTAAATCCGAACAACGCTAGCCCCCTTC

[0064] GTATTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACCGTCATT

[0065] ATCTTCACCGGTGAAAGAGCTTTACAACCCTAGGGCCTTCATCACTCACGCGGCATGG

[0066] CTGGATCAGGCTTGCGCCCATTGTCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCT

[0067] GGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCAGACCAGCTATGGATCGTC

[0068] GCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCCAACGCGGGCTCATCATTTGCCG

[0069] ATAAATCTTTCCCCCAAAGGGCACATACGGTATTAGCAGTCGTTTCCAACTGTTGTTCC

[0070] GTAGCAAATGGTAGATTCCCACGCGTTACTCACCCGTCTGCCGCTCCCCTTGCGGGGC

[0071] GCTCGACTGCAGGTAAGC

[0072] The phylogenetic tree is made by using the 16S rRNA gene sequence of DY-10 and the 16S rRNA gene sequences with high similarity, so that the homology result between the 16S rRNA gene of DY-10 and the 16S rRNA gene with high similarity is obtained. The phylogenetic tree constructed by the adjacent connection method is shown in Figure 2 At present, there are few reports on the application of the strain in the field of environment. Therefore, the high-efficiency phenanthrene and benzopyrene degrading bacteria have important theoretical and practical significance for the treatment and deep repair of the soil polluted by phenanthrene and benzopyrene and PAHs pollution.

[0073] The above results show that the strain DY-10 separated by the application is the species of Ochrobactrum teleogrylli, which is named as Ochrobactrum teleogrylli DY-10, and is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on February 26, 2025, the address is No. 59 Building, 5th Floor, Guangzhou, Guangdong, China, the postcode is 510070, and the preservation number is GDMCC No: 65988.

[0074] Example 2: Growth conditions of Ochrobactrum teleogrylli DY-10

[0075] 1. Determination of growth temperature: configure the liquid nutrient medium required for the growth of the strain, and sterilize the medium after preparation using a sterilization pot. The activated strain is inoculated into the medium (experimental group), and the medium without inoculation of bacteria is used as a control (control group). The medium is cultured at different temperatures for 12 hours. The control group and each temperature corresponding experimental group have three repeats. The growth of bacteria needs to be observed every day. When the result is difficult to distinguish with the naked eye, the visible-ultraviolet spectrophotometer is used to measure the absorbance of the medium at a wavelength of 600 nm. Finally, the growth temperature and the optimum growth temperature range of the new strain are obtained. The test temperature is as follows: 13℃, 18℃, 23℃, 28℃, 33℃, 38℃.

[0076] 2. Determination of Growth pH: Prepare the liquid nutrient medium required for bacterial growth, and adjust the pH of the culture medium using the following buffer systems: pH 4.0-5.0, 0.1 mol / L sodium citrate and 0.1 mol / L citric acid; pH 6.0-8.0, 0.1 mol / L NaOH and 0.1 mol / L KH2PO4; pH 9.0-10.0, 0.1 mol / L NaHCO3 and 0.1 mol / L Na2CO3; pH 11.0, 0.1 mol / L NaOH and 0.05 mol / L Na2HPO4. Inoculate the bacteria into the culture medium, performing three replicates for each pH. Use uninoculated medium as a control. Incubate the medium at the optimal temperature for new bacterial growth for 12 hours, observing the bacterial growth. When results are difficult to distinguish with the naked eye, measure the absorbance of the medium at a wavelength of λ = 600 nm using a visible-ultraviolet spectrophotometer. Finally, determine the pH at which the new bacteria can grow and the optimal pH range for growth. The pH values ​​tested were as follows: 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0.

[0077] 3. Salt Tolerance: Prepare the liquid nutrient medium required for bacterial growth and adjust the salt concentration. Inoculate the activated new bacteria into the sterilized medium, performing triplicate for each salt concentration. Use uninoculated medium as a control. Incubate the medium at the optimal salinity for bacterial growth for 12 hours and observe bacterial growth. When growth is difficult to distinguish visually, use a visible-ultraviolet spectrophotometer to measure the absorbance of the medium at a wavelength of λ = 600 nm to determine the range of salt concentrations the new bacteria can tolerate. The tested salt concentrations are as follows: 0%, 1%, 2%, 3%, 4%, and 5%.

[0078] like Figure 3 As shown, DY-10 can grow in nutrient broth medium at temperatures ranging from 13 to 38°C, with the optimal growth temperature being the enrichment temperature of 28°C. The bacteria can grow at pH levels ranging from 4.0 to 9.0, with the optimal growth pH being 7.0. The bacteria have relatively low salt tolerance and can grow at salt concentrations ranging from 0% to 4%, with the best growth observed at a salt concentration of 1%.

[0079] Example 3: Degradation experiment of phenanthrene and benzo[a]pyrene

[0080] Based on the above experimental results, the optimal growth conditions for the strain were determined to be a temperature of 28℃, pH 7.0, and 1% NaCl. The growth and degradation experiments of strain DY-10 in high concentrations of phenanthrene and benzo[a]pyrene were conducted under these conditions.

[0081] The strain DY-10 in the logarithmic growth phase was inoculated at a 10% inoculation amount (the absorbance OD of the original bacterial solution was 0.20, and the number of cells contained in the original bacterial solution was 1.1 x 10 7 CFU·mL -1 ) into the inorganic salt medium (see Table 1 for the formula, pH 7.0) containing an initial phenanthrene concentration of 50 mg·L -1 or an initial benzopyrene concentration of 25 mg·L -1 , respectively, and was cultured for 7 days at a temperature of 28°C with shaking, and parallel experiments were performed three times. The treatment without the addition of the strain DY-11 was used as a control treatment.

[0082] The samples of each treatment were taken for chemical analysis, and the specific steps were as follows:

[0083] (1) Sample pretreatment: each culture sample was added to dichloromethane for extraction, and 5 μL of a recovery indicator with a concentration of 200 mg / L was added (for phenanthrene and benzopyrene treatment samples, deuterated polycyclic aromatic hydrocarbons were added), and after shaking well, it was transferred into a separatory funnel and left to stand. After layering, the organic phase was collected, and the lower liquid was returned to the flask for repeated extraction with an equal volume of dichloromethane. The combined extract was transferred to a flat-bottom flask containing an appropriate amount of activated copper foil for rotary evaporation, concentrated to about 2 mL, and a small amount of n-hexane (about 5 mL) was added. Rotary evaporation was repeated three times to replace the organic solvent with n-hexane. The concentrated liquid after replacement was purified with a glass-packed column (about 9 mm in diameter). The column packing was 3 cm of 3% deactivated neutral alumina, 3 cm of 3% deactivated silica gel, and 1 cm of anhydrous sodium sulfate from bottom to top. The column was activated with an appropriate amount of n-hexane, and 15 mL of a mixture of n-hexane / dichloromethane (1:1 by volume) was used to elute the packed column, and the eluate was collected in a brown reagent bottle for about 15 mL. Nitrogen blowing was used to concentrate it to about 0.5 mL, and finally it was transferred to a 1.5 mL cell bottle and stored in the freezer. Before instrument analysis, 5 μL of an internal standard, hexamethylbenzene, was added, with a concentration of 200 mg / L.

[0084] (2) Instrument analysis: Agilent 7890 gas chromatograph-5975 mass spectrometer was used to determine the content of PAHs in each treatment sample. The chromatographic column used was Agilent DB 5-MS capillary column (column length 30 m, inner diameter 0.25 mm, membrane thickness 0.25 μm). Agilent 7890 gas chromatograph-5975 mass spectrometer was used to determine the content of PAHs. Agilent DB 5-MS (column length 30 m, inner diameter 0.25 mm, membrane thickness 0.25 μm) capillary column was used for separation and analysis. The data obtained were processed with Agilent chromatography workstation, and the quantification of phenanthrene and benzopyrene was performed with a 6-point calibration curve and internal standard method. The determination of microbial cell concentration used a photoelectric turbidimetry method, and OD was used to represent the optical density value of the bacterial solution sample determined by ultraviolet light at a wavelength of 600 nm.

[0085] According to the GC-MS determination and analysis, it is found that the strain DY-10 can degrade phenanthrene and benzopyrene, and the degradation rates of phenanthrene and benzopyrene can reach more than 70% after the strain DY-10 is cultured in the inorganic salt culture solution containing 50 mg·L -1 concentration of phenanthrene and 25 mg·L -1 concentration of benzopyrene for 7 days. Figure 4 It is shown that the strain DY-10 is a strain which can degrade phenanthrene and benzopyrene and has strong tolerance to the two compounds.

[0086] The above merely describes the preferred embodiments of the present application, and it should be noted that the above preferred embodiments should not be regarded as a limitation to the present application, and the protection scope of the present application should be defined by the scope of the claims. For those skilled in the art, some improvements and refinements can be made without departing from the spirit and scope of the present application, and these improvements and refinements should also be regarded as the protection scope of the present application.

Claims

1. Ochrobactrum teleogrylli DY-10, with the accession number: GDMCC No: 65988.

2. The method of claim 1 Ochrobactrum teleogrylli Use of DY-10 in degrading polycyclic aromatic hydrocarbons, the polycyclic aromatic hydrocarbons being phenanthrene and benzopyrene.

3. Use according to claim 2, characterized in that it is The above-described Ochrobactrum teleogrylli A bacterial suspension of DY-10 was applied to a polycyclic aromatic hydrocarbon contaminated water body or soil.

4. The method of claim 1 Ochrobactrum teleogrylli Use of DY-10 in the preparation of a polycyclic aromatic hydrocarbon-degrading bacterial agent, the polycyclic aromatic hydrocarbon being phenanthrene and benzopyrene.

5. A polycyclic aromatic hydrocarbon-degrading bacterial agent, characterized by comprising the polycyclic aromatic hydrocarbon-degrading bacteria according to any one of claims 1 to 4. comprising the compound of claim 1 Ochrobactrum teleogrylli DY-10 or a pure culture thereof as an active ingredient, the polycyclic aromatic hydrocarbons being phenanthrene and benzopyrene.

6. The method of claim 1 Ochrobactrum teleogrylli Use of the bacterial agent of claim 5 or DY-10 in bioremediation of polycyclic aromatic hydrocarbon contaminated environments, said polycyclic aromatic hydrocarbons being phenanthrene and benzopyrene.

7. Use according to claim 6, characterized in that, The polycyclic aromatic hydrocarbon contaminated environment is a polycyclic aromatic hydrocarbon contaminated water body and / or soil.

8. A method of degrading polycyclic aromatic hydrocarbons, characterized by, The method of claim 1 Ochrobactrum teleogrylli DY-10 or the bacterial agent of claim 5 is applied to a polycyclic aromatic hydrocarbon contaminated environment to degrade polycyclic aromatic hydrocarbons, the polycyclic aromatic hydrocarbons being phenanthrene and benzopyrene.

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