Sucrose soil conditioner as well as preparation method and application thereof in improvement of drought resistance of crops or promotion of growth of crops

Abstract

The invention provides a sucrose soil conditioner, a preparation method thereof and application of the sucrose soil conditioner in improvement of crop drought resistance or promotion of crop growth, and belongs to the technical field of agricultural planting and soil improvement. The invention provides a sucrose soil conditioner. The sucrose soil conditioner is prepared from the following raw materials in parts by weight: 30-40 parts of sucrose, 5-8 parts of organic acid, 3-5 parts of amino acid, 3-5 parts of potassium fulvate and 0.01-0.05 part of a microbial agent. According to the application, the sucrose is taken as a main raw material and is matched with different organic acid and amino acid components, the composition of root exudates is simply simulated, and more drought-resistant beneficial microorganisms can be gathered after the application, and meanwhile, the microorganisms such as lactobacillus plantarum and bacillus velezensis are added; gamma-aminobutyric acid and other growth-promoting substances can be generated to further promote the growth of drought-resistant beneficial microorganisms, so that the drought resistance of plants is enhanced, and the growth of the plants is promoted.

Description

Technical Field

[0001] The present invention belongs to the fields of agricultural planting and soil improvement, and particularly relates to a sucrose soil conditioner, a preparation method thereof, and an application in enhancing the drought resistance of crops or promoting the growth of crops. Background Art

[0002] Drought is one of the main environmental stresses affecting agricultural production. The intensification of global climate change has exacerbated its harm, resulting in crop yield reduction or even crop failure. In recent years, studies have found that plants can regulate the rhizosphere environment through root exudates to adapt to drought stress. Among them, carbohydrates are not only plant metabolites but also can affect the soil microbial community, thereby enhancing the drought resistance of crops. However, the role of sucrose in crop drought resistance and its agricultural application value lack in-depth research. Existing studies have shown that specific rhizosphere microorganisms (such as Nocardiopsis, Pseudomonas, Lysobacter, etc.) can promote plant growth and improve drought resistance. Therefore, how to use exogenous substances to regulate rhizosphere microorganisms and improve crop drought resistance is an important research direction in agricultural biotechnology and also the technical problem that this application hopes to solve. Summary of the Invention

[0003] The purpose of the present invention is to provide a method that can regulate rhizosphere microorganisms and improve the drought resistance of crops.

[0004] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0005] The present invention provides a sucrose soil conditioner, which comprises the following raw materials in parts by weight: 30 - 40 parts of sucrose, 5 - 8 parts of organic acid, 3 - 5 parts of amino acid, 3 - 5 parts of potassium fulvate, and 0.01 - 0.05 parts of microbial inoculum;

[0006] The organic acid includes one or more of oxalic acid, citric acid, succinic acid, malic acid, acetic acid, succinic acid or tartaric acid;

[0007] The amino acid includes lysine, cysteine, tryptophan and glycine.

[0008] Preferably, the mass ratio of lysine, cysteine, tryptophan and glycine is 2 - 3:2 - 3:1 - 2:1 - 2.

[0009] Preferably, the microbial inoculum is Lactobacillus plantarum and Bacillus velezensis, and the ratio of viable bacteria numbers is 2 - 4:1.

[0010] The preparation method of the sucrose soil conditioner comprises the following steps: Put 10 - 15 parts of sucrose and potassium fulvate into a reaction kettle together, add water according to the mass-volume ratio of 1 g:8 - 12 mL, let it stand for 3 - 5 days, then add the microbial inoculum, and culture at 30 - 35 °C until the bacterial concentration is (6 - 8)×108 CFU / mL, and finally adding the remaining sucrose, organic acid and amino acid, culturing for 2 to 3 days and then spray drying to obtain the soil conditioner.

[0011] The present invention also provides the use of the sucrose soil conditioner in improving the drought resistance of crops or promoting the growth of crops.

[0012] The application method of the sucrose soil conditioner is as follows: dissolve the sucrose soil conditioner in water according to a mass volume ratio of 1g:8-12mL, spray it, and the spraying amount is 10-15L / mu, and then spray it again every 10-15 days, and the spraying amount is 3-5L / mu, and spray it 3-5 times in total, and let it stand for more than 10 days after the last spraying.

[0013] The present invention has found through experiments that under drought stress conditions, spring wheat can enhance drought resistance through osmotic regulation, oxidative stress and stomatal regulation, significantly increase the secretion of sugar substances such as sucrose, and recruit a variety of drought-resistant beneficial microorganisms. These microorganisms can enhance plant drought resistance by promoting root growth, enhancing nutrient supply, regulating osmotic substances, reducing ethylene levels and antioxidant protection. Therefore, this application chooses sucrose as the main raw material, with a small amount of organic acids and amino acids and other ingredients, simply simulates the composition of root secretions, in order to gather more drought-resistant beneficial microorganisms, and adds microorganisms such as plant lactobacillus and Bacillus velezii, which can produce probiotic substances such as γ-aminobutyric acid to further promote the growth of drought-resistant beneficial microorganisms, thereby enhancing plant drought resistance. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0015] Figure 1 is the change of microbial Alpha diversity index;

[0016] Figure 2 is the soil microbial community composition and relative abundance;

[0017] Figure 3 This is a comparison of the phenotypes of spring wheat planted in different treatment groups in Example 3. The left side is under control conditions, and the right side is under drought treatment conditions. DETAILED DESCRIPTION

[0018] Lactobacillus plantarum, purchased from Shandong Pingju Biotechnology Co., Ltd., 10 billion / g, and Bacillus velezensis, purchased from Qingdao Yihao Biotechnology Co., Ltd., 20 billion / g.

[0019] The technical solutions provided by the present invention will be described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0020] Example 1

[0021] The test variety was the new spring wheat variety Dingfeng 18. In this experiment, from the tillering stage to the maturity stage of spring wheat, 2 water treatments were set, namely normal water supply (field water holding capacity in the 0-20 cm soil layer was 75%-80%, CK group) and severe soil drought (field water holding capacity in the 0-20 cm soil layer was 45%-50%, drought stress group). A 1.0 m cement isolation belt was set between each plot of different treatments to prevent lateral leakage and infiltration of water. Then, the contents of different components in the root exudates of spring wheat under the control (CK) and drought stress treatments were analyzed.

[0022] Table 1 Analysis of representative components in the root exudates of spring wheat under drought stress treatment

[0023]

[0024] As shown in Table 1, the relative abundance of sucrose in the root exudates of wheat plants was the highest, especially under the drought stress treatment, and its abundance reached 27.49%. The relative abundance of uridine was the lowest. This result indicates that sucrose plays an important role in the drought resistance and growth and development of spring wheat.

[0025] Example 2

[0026] To explore whether host plants promote drought resistance by actively recruiting rhizosphere microbiomes, the following experiment was set up:

[0027] Soil was dug from the experimental field and placed in flower pots with a radius of 5 cm and a height of 15 cm. The soil was treated equally with sterile water, sucrose solution (mass concentration of 10%), and uridine solution (mass concentration of 10%) to keep the soil water content at 70-75%. The soil treated with uridine (Uridine) and sterile water (Sterile Water) was used as the negative control. Treating with sterile water was mainly to exclude the influence of soil humidity changes on the microbial community.

[0028] After two months of cultivation, the amplified sequencing of the cultivated soil was carried out to analyze the microbial community characteristics. The results showed that the Alpha diversity of bacteria in the soil treated with sucrose was significantly higher than that in the uridine treatment group (P<0.01, Figure 1)。 Further analysis of the composition and relative abundance of the soil microbial community revealed that compared with the uridine treatment, the relative abundances of Pseudonocardia, Pseudomonas, Lysobacter, Phycicoccus, and Azohydromonas in the soil treated with sucrose were all significantly increased ( Figure 2 )。

[0029] Example 3

[0030] Using spring wheat as the experimental material, the soil treated with sucrose solution, uridine solution, and sterile water in Example 2 was divided into two groups: a normal water supply group and a drought stress group for spring wheat cultivation. A pot experiment was carried out for the normal water supply group (ensuring a soil water holding capacity of 70 - 75% during the planting process) and the drought stress group (ensuring a soil water holding capacity of 45% - 50% during the planting process).

[0031] The results are as Figure 3 shown. Under normal ( Figure 3 left) and drought treatments ( Figure 3 right), the growth trend of spring wheat in the sucrose treatment group was significantly better than that in the uridine and sterile water treatment groups.

[0032] In summary, under drought stress conditions, spring wheat may enhance its drought resistance through osmotic adjustment, oxidative stress, and stomatal regulation, significantly increasing the secretion of sugars such as sucrose, and recruiting drought-resistant beneficial microorganisms including Pseudonocardia, Pseudomonas, Lysobacter, Phycicoccus, and Azohydromonas. These microorganisms can enhance plant drought resistance by promoting root growth, enhancing nutrient supply, regulating osmotic substances, reducing ethylene levels, and providing antioxidant protection. Therefore, in this application, sucrose is selected as the main raw material, combined with a small amount of organic acids and amino acids, etc., to simply simulate the composition of root exudates to aggregate more drought-resistant beneficial microorganisms. At the same time, microorganisms such as Lactobacillus plantarum and Bacillus velezensis are added, which can produce promoting substances such as γ-aminobutyric acid to further promote the growth of drought-resistant beneficial microorganisms, thereby enhancing plant drought resistance.

[0033] Example 4

[0034] A sucrose soil conditioner:

[0035] Put 15 parts of sucrose and 5 parts of fulvic acid potassium into a reaction kettle, add water according to the mass-volume ratio of 1 g:10 mL of the total mass of sucrose and fulvic acid potassium, and let it stand for 3 days. Then add 0.03 parts of Lactobacillus plantarum and 0.01 parts of Bacillus velezensis, and culture at 30 °C until the bacterial concentration reaches 8×10 8 CFU / mL. Finally, add 25 parts of sucrose, 2 parts of oxalic acid, 2 parts of citric acid, 2 parts of succinic acid, 2 parts of malic acid, 1 part of lysine, 1 part of cysteine, 0.5 part of tryptophan and 0.5 part of glycine. After mixing, let it stand and react for 1 day, and then carry out spray drying (inlet air temperature 150 °C, tower temperature 85 °C, vacuum degree -0.02 MPa, outlet air temperature 80 °C) to obtain the soil conditioner.

[0036] Example 5

[0037] A sucrose soil conditioner:

[0038] Put 10 parts of sucrose and 5 parts of fulvic acid potassium into a reaction kettle, add water according to the mass-volume ratio of 1 g:10 mL of the total mass of sucrose and fulvic acid potassium, and let it stand for 5 days. Then add 0.04 parts of Lactobacillus plantarum and 0.01 parts of Bacillus velezensis, and culture at 35 °C until the bacterial concentration reaches 6×10 8 CFU / mL. Finally, add 20 parts of sucrose, 2 parts of oxalic acid, 2 parts of citric acid, 1 part of acetic acid, 1 part of succinic acid, 1.5 parts of lysine, 1.5 parts of cysteine, 0.5 part of tryptophan and 0.5 part of glycine. After mixing, let it stand and react for 1 day, and then carry out spray drying (inlet air temperature 150 °C, tower temperature 85 °C, vacuum degree -0.02 MPa, outlet air temperature 80 °C) to obtain the soil conditioner.

[0039] Example 6

[0040] A sucrose soil conditioner:

[0041] Put 15 parts of sucrose and 5 parts of fulvic acid potassium into a reaction kettle, add water according to the mass-volume ratio of 1 g:10 mL of the total mass of sucrose and fulvic acid potassium, and let it stand for 3 days. Then add 0.04 parts of Lactobacillus plantarum and 0.01 parts of Bacillus velezensis, and culture at 35 °C until the bacterial concentration reaches 8×10 8 CFU / mL. Finally, add 25 parts of sucrose, 2 parts of oxalic acid, 2 parts of citric acid, 2 parts of acetic acid, 2 parts of tartaric acid, 1.5 parts of lysine, 1.5 parts of cysteine, 1 part of tryptophan and 1 part of glycine. After mixing, let it stand and react for 1 day, and then carry out spray drying (inlet air temperature 150 °C, tower temperature 85 °C, vacuum degree -0.02 MPa, outlet air temperature 80 °C) to obtain the soil conditioner.

[0042] Example 7

[0043] Dissolve the soil conditioner described in Example 6 in water at a mass - volume ratio of 1 g:10 mL, spray it on the soil surface, with a spraying amount of 10 L per mu, then spray it once every 10 days for a total of 4 times, with a supplementary spraying amount of 3 L per mu each time, and plow the soil once after each spraying, with a depth of 20 cm. After the last spraying, let it stand for 15 days.

[0044] Control Example 1

[0045] Different from Example 7, the sucrose in the soil conditioner used in this control example is replaced by trehalose at the same concentration.

[0046] Control Example 2

[0047] Different from Example 7, the microbial inoculant is not added to the soil conditioner used in this control example.

[0048] Field experiment

[0049] Design four groups of experiments. One group is the normal water - supply group (CK1 group), without soil improvement before planting, and the field water - holding capacity of the 0 - 20 cm soil layer is 75% - 80% during the planting process;

[0050] The other three groups are drought - stress groups, and the field water - holding capacity of the 0 - 20 cm soil layer is 45% - 50% during the planting process. Among them, two groups are treated and improved according to the methods of Example 7 and Control Example 1 respectively before planting, and the other group only applies an equal amount of clear water (CK2 group) before the planting experiment. Conduct three parallel experiments. Set a 1.0 m cement isolation belt between each plot of different treatments to prevent lateral leakage and infiltration of water. The area of each plot is 0.5 mu. The test variety is the new spring wheat variety Dingfeng No. 18. Two months after sowing, randomly select 6 wheat plants in each test area, and count the plant height and the dry weight of the above - ground part of the wheat. The results are shown in Table 2.

[0051] Table 2 Application of different soil conditioners to plant growth

[0052] CK1 Example 7 Comparative Example 1 Comparative Example 2 CK2 Plant height / cm 20.21±1.24 19.65±0.47 18.11±0.72 17.85±1.15 16.42±1.15 Dry weight / g 0.0281 0.0267 0.0234 0.0242 0.0188

[0053] As shown in Table 2, under drought conditions, after using the sucrose soil conditioner provided by the present application to improve the soil, the growth of spring wheat plants is significantly improved. The plant height of the sucrose soil conditioner group is about 8% higher than that of other sugar components, 10% higher than that of the treatment group without using the inoculant, and 19.60% higher than that of the CK2 group. At the same time, the dry weight of the above - ground part of the plants in the sucrose soil conditioner group is increased by 42% compared with the CK2 group, 14.1% higher than that of the soil conditioner groups with other sugar components, and 10% higher than that of the treatment group without adding the inoculant. These results indicate that under drought stress, using the sucrose soil conditioner provided by the present invention to improve the soil helps to increase the plant height and dry weight of spring wheat during the subsequent planting process and promote the growth and development of plants.

[0054] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A sucrose soil conditioner, characterized in that: The method comprises the following raw materials in parts by weight: 30-40 parts of sucrose, 5-8 parts of organic acid, 3-5 parts of amino acid, 3-5 parts of potassium humate, and 0.01-0.05 parts of microbial agent; The organic acid includes one or more of oxalic acid, citric acid, succinic acid, malic acid, acetic acid, succinic acid or tartaric acid; The amino acids include lysine, cysteine, tryptophan and glycine.

2. The sucrose soil conditioner according to claim 1, characterized in that: The mass ratio of lysine, cysteine, tryptophan and glycine is 2-3:2-3:1-2:1-2.

3. The sucrose soil conditioner according to claim 1, characterized in that: The microbial agents are Lactobacillus plantarum and Bacillus velezinus, and the ratio of the number of live bacteria is 2-4:

1.

4. The method for preparing the sucrose soil conditioner according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: putting 10 to 15 parts of sucrose and potassium humate into a reaction kettle, adding water according to a mass volume ratio of 1 g: 8 to 12 mL, and standing for 3 to 5 days, then adding a microbial agent, and culturing at 30 to 35° C. until the bacterial concentration is (6 to 8)×10 8 CFU / mL, and finally adding the remaining sucrose, organic acid and amino acid to continue culturing for 1 to 2 days and then spray drying to obtain the sucrose soil conditioner.

5. Use of the sucrose soil conditioner according to any one of claims 1 to 3 or the sucrose soil conditioner prepared by the preparation method according to claim 4 in improving the drought resistance of crops or promoting the growth of crops.

6. The use according to claim 5, characterized in that: The application method of the sucrose soil conditioner is as follows: dissolve the sucrose soil conditioner in water according to a mass volume ratio of 1g:8-12mL, spray it, the spraying amount is 10-15L / mu, spray it again every 10-15 days, the spraying amount is 3-5L / mu, spray it 3-5 times in total, and let it stand for more than 10 days after the last spraying.

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