Nano micro-emulsion co-wrapping puerarin, sophocarpidine and curcumin, preparation method and application of nano micro-emulsion, and oil-control cosmetic

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a nanometer microemulsion co-wrapping puerarin, sophocarpidine and curcumin, a preparation method and application of the nanometer microemulsion and an oil control cosmetic. The nano microemulsion is prepared from the following raw materials: curcumin, sophocarpidine, puerarin, lecithin, hydrogenated lecithin, a surfactant, a solvent and water. The invention not only solves the problem that the solubility difference of curcumin, matrine and puerarin is huge, but also solves the problems that curcumin is easy to decompose, matrine and puerarin are poor in water solubility and ester solubility and difficult to be applied to an aqueous solution system, and the pretreatment wastes time and labor; and a theoretical basis is provided for application of the puerarin, sophocarpidine and curcumin composition in soothing and acne-removing cosmetics. The nano microemulsion co-wrapping puerarin, sophocarpidine and curcumin disclosed by the invention has excellent properties of inhibiting grease secretion of oily sensitive skin and repairing skin barrier, is simple in components and preparation process, and is suitable for industrial production.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a nanoemulsion co-encapsulating puerarin, matrine and curcumin, a preparation method and application thereof, and an oil-control cosmetic. Background Art

[0002] Sebum mainly comes from keratinocytes and sebaceous glands. In the final stage of differentiation, keratinocytes can synthesize cholesterol, fatty acids and ceramides. These lipids can form a stratified lamellar structure between keratinocytes, so it is also called physiological sebum; the sebaceous glands secrete a mixture of various lipids, including squalene, monoester wax, free fatty acids, triglycerides, glycerol ether diesters, cholesterol and cholesterol esters, etc. They come to the skin surface through hair follicles, and are also called free sebum. Appropriate sebum secretion has positive effects. However, excessive sebum secretion will also bring certain negative impacts. Under the combined influence of internal hormone levels and external stimulating factors, the sebaceous glands of oily skin often show an abnormally active state. Excessive oil accumulates in the pores, only squeezing the pores larger and larger. The oil wrapped in the skin will turn into whitehead acne; when it breaks out of the skin and oxidizes in the air and turns black, it will form blackhead acne, and in more serious cases, it will even deteriorate into acne. The definition of oil control in the "Cosmetics Classification Rules and Classification Catalogue" is "helping to slow down the sebum secretion and deposition at the application site, or making the oiliness at the application site less obvious".

[0003] One of the existing oil-control technologies is the most common method at present, which is to remove a large amount of oil on the skin surface by means of strong cleansing, blotting paper or oil-absorbing powder, etc., so as to achieve the effect of clean and fresh skin. This method is deeply loved by consumers because of its quick effect and convenience. However, this method can only temporarily control the oil on the skin surface and cannot achieve the effect of treating both the symptoms and the root causes. Another existing oil-control technology is to try to shrink pores in order to reduce the excessive flow of oil to the skin surface, thereby improving the effect of a shiny face. However, for skin types with overly active sebum secretion, it is more likely to cause pore blockage, resulting in inflammation and acne. The third existing oil-control technology is to use zinc salts to inhibit sebum metabolism. However, relying solely on zinc salts cannot achieve satisfactory results. At the same time, the solubility and ionic nature of zinc salts pose great limitations to the product production process and also cause many adverse effects on the product and its stability.

[0004] As disclosed in Chinese Patent Publication No. CN107126379A, an oil-control and acne-removing composition and its application are provided. The oil-control and acne-removing composition comprises the following components in parts by weight: 2-25 parts of water-soluble polyurethane, 5-25 parts of kaolin, 1-5 parts of titanium dioxide, 3-10 parts of dipropylene glycol, 0.1-1 part of zinc pyrrolidone carboxylate, and 1-5 parts of acne-removing active substance. The above oil-control and acne-removing composition inhibits 5α-reductase, suppresses sebum secretion, effectively inhibits Propionibacterium acnes in acne-prone skin, and effectively repairs acne caused by oily skin.

[0005] Another Chinese Patent Publication No. CN112516066A discloses an oil-control composition and its application in cosmetics. The oil-control composition comprises, by weight percentage: 30-80% of a solvent, 1-40% of fruit acid, 1-45% of Aloe barbadensis Miller leaf extract, 1-25% of Clerodendranthus spicatus extract, 1-10% of plankton extract, and 1-25% of white willow bark extract. The oil-control composition of the present invention has the effects of dredging hair follicle sebaceous glands, controlling sebum secretion, inhibiting inflammatory reactions and the reproduction of Propionibacterium acnes, and improving skin condition.

[0006] However, the compositions prepared by the above-mentioned invention patents are still relatively limited in controlling sebum secretion, and have complex components and long processes, which are not suitable for industrial production. In view of this, there is an urgent need in the art to develop a composition with stronger sebum secretion control ability and simple components. Summary of the Invention

[0007] In view of the problems existing in the prior art, the present invention provides a nanoemulsion co-encapsulating puerarin, matrine and curcumin, its preparation method and application, and an oil-control cosmetic.

[0008] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] A nanoemulsion co-encapsulating puerarin, matrine and curcumin, comprising the following raw materials: curcumin, matrine, puerarin, lecithin, hydrogenated lecithin, surfactant, solvent and water.

[0010] Preferably, the surfactant comprises a mixture of triglyceride caprylate / caprate, polyglyceryl-10 myristate and tocopheryl acetate in a mass ratio of 10-15:2-4:1-2.

[0011] More preferably, the mass ratio of triglyceride caprylate / caprate, polyglyceryl-10 myristate and tocopheryl acetate is 12-15:2-3:1-1.5.

[0012] Further preferably, the mass ratio of triglyceride caprylate / caprate, polyglyceryl-10 myristate and tocopheryl acetate is 15:2:1.

[0013] Preferably, the solvent includes glycerol and ethoxydiglycol.

[0014] Preferably, the mass ratio of glycerol to ethoxydiglycol is 2 - 3:1 - 2.

[0015] Preferably, by mass parts, it includes the following raw materials: 3 - 7 parts of curcumin, 2 - 5 parts of matrine, 1 - 3 parts of puerarin, 1 - 2 parts of lecithin, 1 - 2 parts of hydrogenated lecithin, 18 - 19.5 parts of surfactant, 32 - 45 parts of solvent and 15 - 30 parts of water.

[0016] More preferably, by mass parts, it includes the following raw materials: 3 - 5 parts of curcumin, 4 - 5 parts of matrine, 1 - 2 parts of puerarin, 1 - 2 parts of lecithin, 1 - 2 parts of hydrogenated lecithin, 18 - 19 parts of surfactant, 32 - 34 parts of solvent and 25 - 30 parts of water.

[0017] Further preferably, by mass parts, it includes the following raw materials: 3 parts of curcumin, 4 parts of matrine, 2 parts of puerarin, 2 parts of lecithin, 2 parts of hydrogenated lecithin, 19 parts of surfactant, 32 parts of solvent and 30 parts of water.

[0018] The present invention also provides a preparation method of the above nano - microemulsion, which includes the following steps:

[0019] (1) Mix curcumin, lecithin, surfactant and part of water evenly to obtain a phospholipid microemulsion phase;

[0020] (2) Then mix matrine, puerarin, hydrogenated lecithin, solvent and the remaining water evenly to obtain a hydrogenated lecithin microemulsion phase;

[0021] (3) Finally, mix the phospholipid microemulsion phase with the hydrogenated lecithin microemulsion phase and homogenize to obtain the product.

[0022] Preferably, in steps (1) and (2), the mixing needs to be stirred, the temperature of the stirring is 60 - 70°C, the rotation speed of the stirring is 100 - 200 rpm, and the time of the stirring is 3 - 5 min.

[0023] More preferably, the temperature of the stirring is 70°C, the rotation speed of the stirring is 150 rpm, and the time of the stirring is 5 min.

[0024] Preferably, the homogenization process in step (3) includes first performing high - shear homogenization at 60 - 70°C with a rotation speed of 6000 - 8000 rpm for 3 - 5 min and circulating 2 times; then performing micro - jet high - pressure homogenization treatment at 50 - 60°C and 20000 - 30000 psi and circulating 1 - 3 times to obtain the product.

[0025] More preferably, the homogenization process includes first performing high-shear homogenization at 7000 rpm for 5 min at 60°C and circulating 2 times; then performing microfluidic high-pressure homogenization treatment at 60°C and 30000 psi and circulating 2 times to obtain the product.

[0026] The present invention provides a method for co-encapsulating curcumin, matrine, and puerarin with significantly different dissolution properties. First, curcumin is encapsulated with lecithin to form a phospholipid microemulsion phase, and then matrine and puerarin are encapsulated with hydrogenated lecithin to form a hydrogenated lecithin microemulsion phase. Subsequently, the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase are mixed and sheared by high-shear homogenization to prepare a co-encapsulated primary emulsion. After the co-encapsulated primary emulsion is treated by microfluidic homogenization, a nano-microemulsion co-encapsulating puerarin, matrine, and curcumin is obtained.

[0027] The present invention also provides an oil-control cosmetic, including the above nano-microemulsion or the nano-microemulsion prepared by the above preparation method.

[0028] The present invention also provides the application of the above nano-microemulsion or the nano-microemulsion prepared by the above preparation method in the preparation of products for inhibiting skin oil secretion.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] (1) The present invention not only solves the problem of significant differences in the solubility of curcumin, matrine, and puerarin, but also solves the problems that curcumin is easily decomposed, and the water solubility and ester solubility of matrine and puerarin are poor, making it difficult to be applied in an aqueous system and the pretreatment is time-consuming and laborious, providing a theoretical basis for the application of the puerarin, matrine, and curcumin composition in soothing acne-removing cosmetics.

[0031] (2) The nano-microemulsion co-encapsulating puerarin, matrine, and curcumin provided by the present invention has excellent performance in inhibiting the oil secretion of oily sensitive skin and repairing the skin barrier, and the components and preparation process are simple, suitable for industrial production. Description of the Drawings

[0032] Figure 1 It is the transmission electron microscope morphological observation result of the nano-microemulsion prepared in Example 1 on the 1st day.

[0033] Figure 2 It is the transmission electron microscope morphological observation result of the nano-microemulsion prepared in Example 1 on the 28th day.

[0034] Figure 3 It is the particle size test result.

[0035] Figure 4 It is the Zeta potential test result. Detailed Embodiments

[0036] It should be noted that the raw materials used in the present invention are all commercially available common products. Among them, curcumin with a purity of 99% is purchased from Nanjing Niubang Biotechnology Co., Ltd.; matrine with a purity of 99% is purchased from Guangzhou Xufan Biotechnology Co., Ltd.; puerarin with a purity of 99% is purchased from Wuhan Lanaibai Pharmaceutical and Chemical Co., Ltd.

[0037] Example 1

[0038] A preparation method of a nano - microemulsion co - encapsulating puerarin, matrine and curcumin is as follows:

[0039] (1) By mass, first take 5 parts of curcumin, 15 parts of triglyceride caprylate / caprate, 1 part of lecithin, 2 parts of polyglyceryl - 10 myristate, 1 part of tocopheryl acetate and 20 parts of purified water, and stir at 150 rpm for 5 min in a 60 °C constant - temperature water bath to obtain a phospholipid microemulsion phase;

[0040] (2) Then take 28 parts of glycerol, 1 part of hydrogenated lecithin, 15 parts of ethoxydiglycol, 5 parts of matrine, 1 part of puerarin and 5 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant - temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0041] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase. First, perform high - shear homogenization at 7000 rpm for 5 min at 60 °C, and cycle 2 times; then perform micro - jet high - pressure homogenization treatment at 60 °C and 30000 psi, and cycle 3 times to obtain the product.

[0042] Example 2

[0043] A preparation method of a nano - microemulsion co - encapsulating puerarin, matrine and curcumin is as follows:

[0044] (1) By mass, first take 7 parts of curcumin, 15 parts of triglyceride caprylate / caprate, 1.5 parts of lecithin, 3 parts of polyglyceryl - 10 myristate, 1.5 parts of tocopheryl acetate and 10 parts of purified water, and stir at 100 rpm for 3 min in a 65 °C constant - temperature water bath to obtain a phospholipid microemulsion phase;

[0045] (2) Then take 30 parts of glycerol, 1.5 parts of hydrogenated lecithin, 15 parts of ethoxydiglycol, 2 parts of matrine, 3 parts of puerarin and 5 parts of purified water, and stir at 200 rpm for 5 min in a 60 °C constant - temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0046] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase. First, perform high - shear homogenization at 6500 rpm for 3 min at 70 °C, and cycle 2 times; then perform micro - jet high - pressure homogenization treatment at 50 °C and 25000 psi to obtain the product.

[0047] Example 3

[0048] A preparation method of a nano - microemulsion co - encapsulating puerarin, matrine and curcumin is as follows:

[0049] (1) By mass fraction, first take 3 parts of curcumin, 13 parts of glyceryl caprylate / caprate, 2 parts of lecithin, 4 parts of polyglyceryl - 10 myristate, 2 parts of tocopheryl acetate and 20 parts of purified water, and stir at 200 rpm for 5 min in a 70 °C constant - temperature water bath to obtain a phospholipid microemulsion phase;

[0050] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 4 parts of matrine, 2 parts of puerarin and 10 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant - temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0051] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase, first perform high - shear homogenization at 8000 rpm for 5 min at 65 °C, and cycle 2 times; then perform micro - jet high - pressure homogenization treatment at 20000 psi at 60 °C, and cycle 2 times to obtain the product.

[0052] Comparative Example 1

[0053] Compared with Example 3, the difference is only that glyceryl caprylate / caprate is replaced by ethylhexyl palmitate.

[0054] A preparation method of a nano - microemulsion co - encapsulating puerarin, matrine and curcumin is as follows:

[0055] (1) By mass fraction, first take 3 parts of curcumin, 13 parts of ethylhexyl palmitate, 2 parts of lecithin, 4 parts of polyglyceryl - 10 myristate, 2 parts of tocopheryl acetate and 20 parts of purified water, and stir at 200 rpm for 5 min in a 70 °C constant - temperature water bath to obtain a phospholipid microemulsion phase;

[0056] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 4 parts of matrine, 2 parts of puerarin and 10 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant - temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0057] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase, first perform high - shear homogenization at 8000 rpm for 5 min at 65 °C, and cycle 2 times; then perform micro - jet high - pressure homogenization treatment at 20000 psi at 60 °C, and cycle 2 times to obtain the product.

[0058] Comparative Example 2

[0059] Compared with Example 3, the difference is only that glycerol is replaced by butanediol.

[0060] A preparation method of a nanoemulsion co-encapsulating puerarin, matrine and curcumin, the steps are as follows:

[0061] (1) By mass, first take 3 parts of curcumin, 13 parts of glyceryl caprylate / caprate, 2 parts of lecithin, 4 parts of polyglyceryl-10 myristate, 2 parts of tocopheryl acetate and 20 parts of purified water, and stir at 200 rpm for 5 min in a 70 °C constant temperature water bath to obtain a phospholipid nanoemulsion phase;

[0062] (2) Then take 24 parts of butanediol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 4 parts of matrine, 2 parts of puerarin and 10 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant temperature water bath to obtain a hydrogenated lecithin nanoemulsion phase;

[0063] (3) Finally, mix the phospholipid nanoemulsion phase and the hydrogenated lecithin nanoemulsion phase, first perform high-shear homogenization at 8000 rpm for 5 min at 65 °C, and circulate 2 times; then perform microfluidic high-pressure homogenization treatment at 20000 psi at 60 °C, and circulate 2 times to obtain the product.

[0064] Comparative Example 3

[0065] Compared with Example 3, the difference is only that step (3) is different.

[0066] A preparation method of a nanoemulsion co-encapsulating puerarin, matrine and curcumin, the steps are as follows:

[0067] (1) By mass, first take 3 parts of curcumin, 13 parts of glyceryl caprylate / caprate, 2 parts of lecithin, 4 parts of polyglyceryl-10 myristate, 2 parts of tocopheryl acetate and 20 parts of purified water, and stir at 200 rpm for 5 min in a 70 °C constant temperature water bath to obtain a phospholipid nanoemulsion phase;

[0068] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 4 parts of matrine, 2 parts of puerarin and 10 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant temperature water bath to obtain a hydrogenated lecithin nanoemulsion phase;

[0069] (3) Finally, mix the phospholipid nanoemulsion phase and the hydrogenated lecithin nanoemulsion phase, first perform microfluidic high-pressure homogenization treatment at 20000 psi at 60 °C, and circulate 2 times; then perform high-shear homogenization at 8000 rpm for 5 min at 65 °C, and circulate 2 times to obtain the product.

[0070] Comparative Example 4

[0071] Compared with Example 3, the difference is only that it does not contain curcumin.

[0072] A preparation method of a nanoemulsion, the steps are as follows:

[0073] (1) By mass fraction, first take 13 parts of triglyceride caprylate / caprate, 2 parts of lecithin, 4 parts of polyglyceryl-10 myristate, 2 parts of tocopheryl acetate, and 20 parts of purified water, stir at 200 rpm for 5 min in a 70 °C constant temperature water bath to obtain a phospholipid microemulsion phase;

[0074] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 6 parts of matrine, 3 parts of puerarin, and 10 parts of purified water, stir at 150 rpm for 5 min in a 70 °C constant temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0075] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase, first perform high-shear homogenization at 8000 rpm for 5 min at 65 °C, circulate 2 times; then perform microfluidic high-pressure homogenization treatment at 60 °C and 20000 psi, circulate 2 times to obtain.

[0076] Comparative Example 5

[0077] Compared with Example 3, the difference is only that it does not contain puerarin and matrine.

[0078] A method for preparing a nano-microemulsion, the steps are as follows:

[0079] (1) By mass fraction, first take 9 parts of curcumin, 13 parts of triglyceride caprylate / caprate, 2 parts of lecithin, 4 parts of polyglyceryl-10 myristate, 2 parts of tocopheryl acetate, and 20 parts of purified water, stir at 200 rpm for 5 min in a 70 °C constant temperature water bath to obtain a phospholipid microemulsion phase;

[0080] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, and 10 parts of purified water, stir at 150 rpm for 5 min in a 70 °C constant temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0081] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase, first perform high-shear homogenization at 8000 rpm for 5 min at 65 °C, circulate 2 times; then perform microfluidic high-pressure homogenization treatment at 60 °C and 20000 psi, circulate 2 times to obtain.

[0082] Comparative Example 6

[0083] Compared with Example 3, the difference is only that it does not contain puerarin.

[0084] A method for preparing a nano-microemulsion, the steps are as follows:

[0085] (1) By mass fraction, first take 3 parts of curcumin, 13 parts of caprylic / capric triglyceride, 2 parts of lecithin, 4 parts of polyglyceryl-10 myristate, 2 parts of tocopheryl acetate and 20 parts of purified water, and stir at 200 rpm for 5 min in a 70 °C constant temperature water bath to obtain a phospholipid microemulsion phase;

[0086] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 6 parts of matrine and 10 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0087] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase, first perform high-shear homogenization at 8000 rpm for 5 min at 65 °C, and circulate 2 times; then perform microfluidic high-pressure homogenization treatment at 60 °C and 20000 psi, and circulate 2 times to obtain.

[0088] Comparative Example 7

[0089] Compared with Example 3, the difference is only that it does not contain matrine.

[0090] A method for preparing a nano-microemulsion, the steps are as follows:

[0091] (1) By mass fraction, first take 3 parts of curcumin, 13 parts of caprylic / capric triglyceride, 2 parts of lecithin, 4 parts of polyglyceryl-10 myristate, 2 parts of tocopheryl acetate and 20 parts of purified water, and stir at 200 rpm for 5 min in a 70 °C constant temperature water bath to obtain a phospholipid microemulsion phase;

[0092] (2) Then take 24 parts of glycerol, 2 parts of hydrogenated lecithin, 8 parts of ethoxydiglycol, 6 parts of puerarin and 10 parts of purified water, and stir at 150 rpm for 5 min in a 70 °C constant temperature water bath to obtain a hydrogenated lecithin microemulsion phase;

[0093] (3) Finally, mix the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase, first perform high-shear homogenization at 8000 rpm for 5 min at 65 °C, and circulate 2 times; then perform microfluidic high-pressure homogenization treatment at 60 °C and 20000 psi, and circulate 2 times to obtain.

[0094] Test Example 1

[0095] Perform transmission electron microscopy morphology, particle size, PDI and Zeta potential tests on the nano-microemulsion co-encapsulating puerarin, matrine and curcumin prepared in Example 1. The test methods are as follows:

[0096] 1. Transmission electron microscopy (TEM) morphology test: The Hitachi HT7800 high-contrast transmission electron microscope was used to observe the surface morphology of the nanoemulsion on the 1st day and the 28th day. Before the test, the samples were diluted to a mass concentration of 0.01% with ultrapure water at pH = 7.0. 10 μL of the diluted sample was dropped on a copper grid. After standing for 2 min, the excess sample around the copper grid was aspirated. Then, the copper grid was negatively stained with a 2.0 wt% uranyl acetate solution for 5 min. The excess staining solution was removed with filter paper and air-dried at room temperature. An appropriate amount of the sample was dropped on the copper grid plate, and then the data was recorded by taking pictures.

[0097] The TEM morphology observation results of the nanoemulsion prepared in Example 1 on the 1st day are as Figure 1 shown, and the TEM morphology observation results on the 28th day are as Figure 2 shown. From Figure 1 and 2 , it can be seen that the nanoemulsion prepared in Example 1 can still maintain a plump bilayer structure after 28 days of storage, indicating that the nanoemulsion prepared in Example 1 has relatively excellent stability.

[0098] 2. Particle size, PDI and Zeta potential test: A nanoparticle size analyzer was used to measure the average particle size, PDI and Zeta potential of the nanoemulsion on the 1st day. First, the sample was diluted 100 times with ultrapure water. The test temperature was set at 25 °C, the scattering angle was 90°, and after scanning 3 times, the average value was used as the measured value. Among them, when measuring the Zeta potential, the sample was not diluted and was directly added to the Zeta potential cell for measurement.

[0099] The particle size test results are as Figure 3 shown. From Figure 3 , it can be seen that the D50 particle size of the nanoemulsion in Example 1 is 142.66 nm, which is similar to the microscopic image obtained by TEM morphology test.

[0100] The Zeta potential test results are as Figure 4 shown. From Figure 4 , it can be seen that the Zeta potential of Example 1 is close to zero, and the potential retention effect on skin cells is more obvious.

[0101] Test Example 2

[0102] By mass percentage, 2% of the nanoemulsions prepared in Examples 1-3 and Comparative Examples 1-7, 0.5% of 1,2-butanediol, 0.5% of 1,2-hexanediol, 0.2% of raspberry ketone, 0.3% of hydrolyzed sodium hyaluronate, 0.1% of acryloyldimethyltaurate / beheneth-25 methacrylate cross-linked polymer, and the balance of water were mixed to prepare an aqueous cosmetic, and the human efficacy test was carried out on this aqueous cosmetic.

[0103] Fifty-five volunteers aged 18 - 40 with oily and sensitive facial skin were selected as subjects and divided into 11 groups with 5 people in each group. The volunteers continuously used the aqueous cosmetics containing Examples 1 - 3 and Comparative Examples 1 - 7 for 168 hours. At 30 minutes, 1 hour, 72 hours, and 168 hours after use, the Sebumeter instrument was used to measure the skin oil content, and the skin oil improvement rate was calculated according to the formula. Not using the nano microemulsion was taken as the blank control group.

[0104] Skin oil improvement rate (%) = 1 - (skin oil content of this time ÷ skin oil content of the first time) × 100%.

[0105] The test results are shown in Table 1. It can be seen from Table 1 that by using the aqueous cosmetic products of Examples 1 - 3 of the present invention, the oil secretion of oily and sensitive skin can be effectively inhibited, and effective short-term and long-term oil control effects can be achieved.

[0106] Table 1 Test data

[0107]

[0108] Note: Compared with Example 3 in each column, * indicates P < 0.05.

[0109] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the protection scope of the present invention. Simple modifications or equivalent replacements made by those of ordinary skill in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A nano-microemulsion co-encapsulating puerarin, matrine and curcumin, characterized in that: The invention comprises the following raw materials: curcumin, matrine, puerarin, lecithin, hydrogenated lecithin, surfactant, solvent and water. The surfactant comprises a mixture of caprylic / capric triglyceride, polyglyceryl-10 myristate and tocopherol acetate in a mass ratio of 10-15:2-4:1-2.

2. The nano-microemulsion according to claim 1, characterized in that: The solvents include glycerin and ethoxydiglycol.

3. The nano-microemulsion according to claim 2, characterized in that: The mass ratio of the glycerol to ethoxydiglycol is 2-3:1-2.

4. The nano-microemulsion according to claim 1, characterized in that: The raw materials are as follows, calculated by weight: 3-7 parts of curcumin, 2-5 parts of matrine, 1-3 parts of puerarin, 1-2 parts of lecithin, 1-2 parts of hydrogenated lecithin, 18-19.5 parts of surfactant, 32-45 parts of solvent and 15-30 parts of water.

5. The nano-microemulsion according to claim 4, characterized in that: The raw materials are as follows, calculated by weight: 3-5 parts of curcumin, 4-5 parts of matrine, 1-2 parts of puerarin, 1-2 parts of lecithin, 1-2 parts of hydrogenated lecithin, 18-19 parts of surfactant, 32-34 parts of solvent and 25-30 parts of water.

6. A method for preparing a nano-microemulsion according to any one of claims 1 to 5, characterized in that: The steps include: (1) mixing curcumin, lecithin, a surfactant and part of water to obtain a phospholipid microemulsion phase; (2) mixing matrine, puerarin, hydrogenated lecithin, solvent and remaining water to obtain a hydrogenated lecithin microemulsion phase; (3) Finally, the phospholipid microemulsion phase and the hydrogenated lecithin microemulsion phase are mixed and homogenized to obtain the product.

7. The preparation method according to claim 6, characterized in that: The mixing in step (1) and step (2) requires stirring, the stirring temperature is 60-70° C., the stirring speed is 100-200 rpm, and the stirring time is 3-5 min.

8. The preparation method according to claim 6, characterized in that: The homogenization process in step (3) includes firstly high shear homogenization at 60-70°C and 6000-8000 rpm for 3-5 min, and repeating twice; and then microfluidization high pressure homogenization at 50-60°C and 20000-30000 psi, and repeating 1-3 times to obtain the product.

9. An oil-control cosmetic, characterized in that: The invention comprises the nano-microemulsion according to any one of claims 1 to 5.

10. Use of the nano-microemulsion according to any one of claims 1 to 5 in preparing a product for inhibiting skin oil secretion.

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