Cell separation filter

Abstract

The invention relates to the technical field of cell filtration, and particularly discloses a cell separation filter which comprises a mounting frame, a filter body is fixedly mounted in the mounting frame, an inner cavity is formed in the filter body, a cell sieve is slidably mounted on the side wall of the inner cavity, a drainage device is fixedly mounted on the side wall of the inner cavity below the cell sieve, and the cell separation filter further comprises a turnover assembly. The liquid dropping assembly and the overturning assembly are matched to perform double-sided infiltration on the cell sieve, so that the cell sieve with the two sides fully infiltrated can reduce friction between a cell suspension and a sieve mesh and prevent cell clusters or tissue blocks from blocking the sieve mesh, thereby improving the filtering efficiency, being beneficial to protecting cells and improving the cell quality. According to the utility model, cell damage caused by drying or friction in the filtering process is reduced, cell activity and integrity are maintained, and the wetted screen enables cell suspension to pass through the screen holes more easily and uniformly, so that local over-fast flow or blockage is avoided, and the filtering uniformity and effect are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell filtration, and specifically to a cell separation filter. Background Art

[0002] A single-cell suspension refers to a mixture in which cells in a tissue sample (such as animal or plant tissue) are separated into individual cells by physical or chemical methods and then dispersed in a liquid medium. The cells in this mixture remain viable and can be used for various biological experiments and analyses. Currently, the prior art usually uses a cell filter to separate primary cells from tissues to obtain a single-cell suspension.

[0003] For example, Chinese Patent No. CN118308197B discloses a cell filter that is convenient for separation and filtration and its filtration method. The device includes a filtration support frame. A filtration cylinder is provided at the bottom of the filtration support frame. A filter screen is fixedly installed at the bottom of the filtration cylinder. Limiting plates are slidably inserted around the upper part of the filtration support frame at equal intervals. When this invention performs cell filtration and impurity removal, it can avoid the situation where cells and culture medium flow to the junction of the filter screen and the filtration cylinder when the cell suction tube is tilted, resulting in cells and culture medium remaining at the junction of the filter screen and the filtration cylinder, ensuring that cells can be completely filtered, avoiding waste of cells, and enabling continuous vibration of the filtration cylinder. The generated vibration can shake the cells and culture medium remaining at the junction of the filter screen and the filtration cylinder onto the filter screen, improving the cleaning effect of the cells and culture medium remaining at the junction of the filter screen and the filtration cylinder.

[0004] However, when filtering a cell suspension through the above-mentioned scheme, due to the large frictional force and shear force between the cells and the filter screen, single cells are prone to cell disk rupture and death due to cell membrane damage when passing through the filter screen, thereby reducing the cell survival rate in the single-cell suspension. Moreover, excessive shear force can affect gene expression and change the original functions and behaviors of cells, which is not conducive to subsequent biological experiments and analyses of cells. Summary of the Invention

[0005] The purpose of the present invention is to provide a cell separation filter to solve at least one of the technical problems existing in the above-mentioned prior art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A cell separation filter includes a mounting frame. A filter is fixedly installed inside the mounting frame. An inner cavity is provided inside the filter. A cell sieve is slidably installed on the side wall of the inner cavity. A drainer is fixedly installed on the side wall of the inner cavity below the cell sieve;

[0007] It further includes a flipping assembly for driving the cell sieve to slide vertically in a reciprocating manner while flipping and switching by 180 degrees;

[0008] The invention also comprises a liquid dripping component, which is used for dripping an infiltration liquid onto the surface of the cell sieve and then passing a cell suspension onto the surface of the cell sieve.

[0009] Preferably, the flip assembly includes two side chambers opened on both sides of the inner chamber, the inner walls of the two side chambers are both installed with fixed blocks that can be adjusted to slide back and forth vertically, the two fixed blocks are both penetrated by a rotating shaft that is rotatably installed, a mounting ring is commonly fixed between the two rotating shafts, the cell screen is fixedly installed on the inner ring of the mounting ring, the side walls of the two fixed blocks are both horizontally slidably installed with a sliding plate of L-shaped design, the outer wall of the sliding plate is rotatably installed with a sliding shaft coaxially arranged with the rotating shaft, the ends of the rotating shaft opposite to the sliding shaft are plugged into each other through a flat key, a gear is fixed to the end of the sliding shaft away from the rotating shaft, and a rack is fixed to the inner wall of the side chamber;

[0010] The flip assembly also includes an adjusting member for controlling the horizontal movement and adjustment of the sliding plate, and when the sliding plate moves, the planes where the gear and the rack are located can be overlapped or staggered.

[0011] Preferably, the adjusting member includes a limit groove opened on the inner wall of the side cavity, the limit groove is formed by splicing a square groove, a right-angled trapezoidal groove and a straight groove and is centrally symmetrical with the midpoint of the square groove, the upper base of the right-angled trapezoidal groove is half of the lower base and is equal to the width of the straight groove, the sliding plate is fixedly installed with a limit pin that can slide in the limit groove, and the diameter of the limit pin is equal to the width of the straight groove, the rack and the gear.

[0012] Preferably, the top surfaces of the two fixed blocks are fixedly installed with limiting frames, and sliding frames are slidably installed in the sliding grooves opened in the two limiting frames. A connecting tube is fixedly installed at the center of the sliding frame, and an elastic bag is fixedly installed on the top of the connecting tube. The top of the elastic bag is connected to the measuring cup on the top surface of the filter through a hose. The connecting tube consists of a hard tube in the upper section and a soft tube in the lower section. The outer wall of the soft tube in the lower section of the connecting tube is provided with an extrusion part for adjusting the flow rate of the liquid in the connecting tube.

[0013] Preferably, the extrusion portion includes a sliding block installed on the outer wall of the hard tube in the upper section of the connecting tube and capable of vertical sliding adjustment. Two groups of fixing frames are fixedly installed on the top surface of the sliding frame. Rollers are rotatably installed at one end of the two fixing frames close to each other, and the main shaft of the roller can slide along the oblique groove opened on the side wall of the fixing frame. The cutting grooves opened on both sides of the sliding block are also provided with oblique grooves for the main shaft of the roller to slide.

[0014] Preferably, a rotating wheel is rotatably installed in a square groove formed on the top surface of the fixed block, a fixed pulley is rotatably installed on the inner top wall of the side cavity, a movable pulley is rotatably installed on one side of the sliding frame, two pulling ropes are fixed on the inner top wall of the side cavity, one end of one of the pulling ropes is connected with a tension spring between the inner bottom wall of the side cavity after sequentially passing around the movable pulley and the fixed pulley, and the other pulling rope is fixedly connected with the axle bracket of the movable pulley after passing around the rotating wheel.

[0015] Preferably, a flow cytometer capable of monitoring cell viability is fixedly installed on the top surface of the mounting bracket below the filter, and the drainer is funnel-shaped and can introduce the cell suspension after passing through the cell sieve into the flow cytometer.

[0016] Preferably, two concave holes are formed on the top surface of the transverse plate of the sliding plate, and elastic pins are slidably installed in the concave holes, and grooves for the elastic pins to insert are provided on the bottom surface of the fixed block.

[0017] Preferably, a slider is slidably installed on the bottom surface of the sliding plate, a cylinder is fixedly installed on the bottom surface of the side cavity, and the end of the main shaft of the cylinder is fixedly connected with the slider.

[0018] Preferably, the inner ring wall of the mounting ring is higher than the top surface of the cell sieve.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] First, the present invention wets one side of the cell sieve through the dropping liquid assembly, and after raising the cell sieve to an appropriate height through the flipping assembly and then flipping it, the cell sieve is wetted again. The cell sieve after being fully wetted on both sides can not only reduce the friction between the cell suspension and the sieve mesh, prevent the blockage of cell clusters or tissue blocks on the sieve mesh, thereby improving the filtration efficiency, but also help to protect cells, reduce cell damage caused by drying or friction during the filtration process, maintain cell viability and integrity, and the wetted sieve mesh makes the cell suspension easier to uniformly pass through the sieve holes, avoiding local rapid flow or blockage, thereby improving the uniformity and effect of filtration.

[0021] Second, by detecting the viability and integrity of the cell suspension after passing through the cell sieve in real time, the present invention can adjust the filtration speed of the cell sieve for the cell suspension through the extrusion part, avoiding that due to the relatively high surface tension of the medium with a certain viscosity when the filtration speed is too fast, the medium passing through the cell sieve drags down or tears the single cells that have not passed through the cell sieve, further enhancing the protection ability of the device for the viability and integrity of single cells, so as to improve the preparation quality of the single cell suspension. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 is a three-dimensional structural schematic diagram of the present invention;

[0023] Figure 2 Cross-sectional view of the three-dimensional structure of the present invention;

[0024] Figure 3 Side cross-sectional view of the present invention;

[0025] Figure 4 In the present invention Figure 3 Isometric cross-sectional view;

[0026] Figure 5 In the present invention Figure 3 Cross-sectional view of the opposite side;

[0027] Figure 6 In the present invention Figure 5 Isometric cross-sectional view;

[0028] Figure 7 For the present invention Figure 4 Local enlarged view at position A in the present invention;

[0029] Figure 8 For the present invention Figure 6 Local enlarged view at position B in the present invention;

[0030] Figure 9 Schematic plan view of the limit groove in the present invention

[0031] Figure 10 Cross-sectional view of the movable pulley and its related structure in the present invention.

[0032] In the figure: 1, mounting frame; 2, filter; 3, flow cytometer; 4, hose; 5, elastic bladder; 6, connecting pipe; 7, sliding block; 8, fixed frame; 9, roller; 10, cell sieve; 11, inner cavity; 12, side cavity; 13, limit groove; 14, cylinder; 15, slider; 16, sliding plate; 17, elastic pin; 18, fixed block; 19, mounting ring; 20, gear; 21, sliding shaft; 22, rotating shaft; 23, rack; 24, sliding frame; 25, pull rope; 26, fixed pulley; 27, movable pulley; 28, rotating wheel; 29, drainer; 30, limit frame. Specific embodiments

[0033] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] Please refer to Figures 1 to 10, the present invention provides a technical solution: a cell separation filter, including a mounting frame 1, a filter 2 is fixedly installed inside the mounting frame 1, an inner cavity 11 is opened inside the filter 2, a cell sieve 10 is slidably installed on the side wall of the inner cavity 11, and a drainer 29 is fixedly installed on the side wall of the inner cavity 11 below the cell sieve 10;

[0035] It further includes a flipping assembly for driving the cell sieve 10 to slide vertically and reciprocally while flipping and switching by 180 degrees;

[0036] It further includes a dropping assembly for dropping an infiltration liquid onto the surface of the cell sieve 10 and then introducing a cell suspension onto the surface of the cell sieve 10.

[0037] When this device is in use, first, an appropriate amount of infiltration liquid (preferably a liquid with the same composition as the cell suspension, such as a culture medium or other nutrients) is dropped onto the surface of the cell sieve 10 through the dropping assembly. After one side of the cell sieve 10 is fully infiltrated, the flipping assembly is used to drive the cell sieve 10 to slide vertically upward and flip by 180 degrees during the sliding process. Subsequently, an appropriate amount of infiltration liquid is continuously dropped onto the surface of the flipped cell sieve 10 through the dropping assembly. After both sides of the cell sieve 10 are fully infiltrated, a cell suspension is introduced onto the cell sieve 10 at an appropriate speed through the dropping assembly. After all the cell suspension has passed through the cell sieve 10 and the filtration is completed, the single-cell suspension obtained after filtration is introduced into a centrifuge cup or other utensils that can be used for centrifugation through the drainer 29. The remaining tissue fragments and undigested cell clumps are separated from the single cells by centrifugation, and the supernatant in the utensils is taken out, and the cell precipitate is retained. Then, the cell precipitate is resuspended with an appropriate buffer or culture medium and adjusted to the required cell concentration, and thus the preparation of the single-cell suspension can be completed.

[0038] In this way, by the dropping assembly, one side of the cell sieve 10 is infiltrated, and the cell sieve 10 is lifted to an appropriate height by the flipping assembly and then flipped, and the cell sieve 10 is infiltrated again. After both sides are fully infiltrated, the cell sieve 10 can not only reduce the friction between the cell suspension and the screen mesh, prevent the blockage of cell clusters or tissue blocks on the screen mesh, thereby improving the filtration efficiency, but also help to protect the cells, reduce cell damage caused by drying or friction during the filtration process, maintain cell viability and integrity, and the moistened screen mesh makes the cell suspension easier to uniformly pass through the screen holes, avoiding local excessive flow or blockage, thereby improving the uniformity and effect of filtration.

[0039] It is worth mentioning that since the infiltration process of the cell sieve 10 is all in the inner cavity 11, it is more conducive to maintaining sterile operation in the process of preparing single-cell suspension, thereby reducing the risk of single-cell suspension being contaminated by external bacterial colonies and flora. After pre-infiltration, the cell sieve 10 can use the capillary action of the fibers in the sieve to slowly spread the infiltration liquid, and cooperate with the vibration generated during its flipping process to expel all the air in the sieve, avoiding the formation of bubbles in the sieve that clog the sieve holes and cause filtering difficulties. The cell sieve 10 is raised and then lowered by the flipping assembly, which reserves sufficient space for the flipping of the cell sieve 10. It can also greatly reduce the height difference between the cell sieve 10 and the drip assembly, and further reduce the impact force or friction between the cells and the sieve when passing through the cell sieve 10, thereby improving the activity and integrity of the cells after filtration.

[0040] Further, the flip assembly includes two side chambers 12 opened on both sides of the inner chamber 11, and the inner walls of the two side chambers 12 are both installed with fixed blocks 18 that can slide back and forth vertically, and the two fixed blocks 18 are both penetrated and rotatably installed with a rotating shaft 22, and a mounting ring 19 is fixed between the two rotating shafts 22. The cell screen 10 is fixedly installed at the inner ring of the mounting ring 19, and the side walls of the two fixed blocks 18 are both horizontally slidably installed with a sliding plate 16 of L-shaped design, and the outer wall of the sliding plate 16 is rotatably installed with a sliding shaft 21 coaxially arranged with the rotating shaft 22, and the ends of the rotating shaft 22 and the sliding shaft 21 opposite to each other are plugged and matched with each other through a flat key, and the end of the sliding shaft 21 away from the rotating shaft 22 is fixed with a gear 20, and the inner wall of the side chamber 12 is fixed with a rack 23;

[0041] The flip assembly also includes an adjusting member for controlling the horizontal movement and adjustment of the sliding plate 16, and when the sliding plate 16 moves, the planes where the gear 20 and the rack 23 are located can be overlapped or staggered.

[0042] According to the above embodiment, a specific embodiment of a flip assembly is provided. When the external assembly drives the two fixing blocks 18 to slide vertically upward together, see FIG. Figure 5, at this time, the fixed block 18 drives the sliding plate 16 and the rotating shaft 22 inside it to slide upward together, causing the gear 20 to mesh with the rack 23. Since the number of teeth of the rack 23 is half that of the gear 20, the gear 20 will rotate 180 degrees when passing through the rack 23. During the process of the mounting ring 19 rising in the inner cavity 11 driven by the sliding shaft 21 and the rotating shaft 22, it will flip 180 degrees. Subsequently, the adjusting member controls the sliding plate 16 to move horizontally along the bottom of the fixed block 18, so that the plane where the gear 20 and the rack 23 are located is offset. At this time, the fixed block 18 slides vertically downward and drives the cell sieve 10 and the mounting ring 19 to slide vertically downward together until the bottom of the mounting ring 19 fits against the top surface of the drainer 29 again, completing the flipping of the cell sieve 10. Then, it can cooperate with the dropping liquid assembly to perform double-sided infiltration on the cell sieve 10 before and after flipping. Subsequently, the adjusting member adjusts the rack 23 and the gear 20 to return to the same plane again. After the filtration is completed, the cell sieve 10 is replaced, and the infiltration of the new cell sieve 10 can be repeated.

[0043] In this way, by driving the fixed block 18 to slide vertically reciprocally through an external component and using the offset or meshing between the rack 23 and the gear 20, the cell sieve 10 can be flipped after rising away from the drainer 29 to an appropriate distance, and the flipping is avoided when the cell sieve 10 descends, so that the cell sieve 10 can closely adhere to the drainer 29 during filtration, thereby preventing the excessive impact force between the cells and the drainer 29 after the cells pass through the cell sieve 10, which may cause the cells to rupture or inactivate, and reducing the cell activity and integrity in the single-cell suspension.

[0044] Furthermore, the adjusting member includes a limiting groove 13 opened on the inner wall of the side cavity 12. The limiting groove 13 is composed of a square groove, a right trapezoidal groove, and a straight groove opening spliced together and is centrosymmetric with the midpoint of the square groove. The upper base of the right trapezoidal groove is half of the lower base and is equal to the width of the straight groove opening. The sliding plate 16 is fixedly installed with a limiting pin that can slide in the limiting groove 13, and the diameter of the limiting pin is equal to the widths of the straight groove opening, the rack 23, and the gear 20.

[0045] According to the above embodiments, a specific embodiment of the adjusting member is provided. For details, see Figure 9In the figure, a is a straight slot, b is a right-angled trapezoidal slot, and c is a square slot. Since the upper base of the right-angled trapezoidal slot is half of the lower base and is equal to the width of the straight slot, and the diameter of the limit pin is equal to the width of the straight slot, the rack 23 and the gear 20, the limit pin will first slide along the leftmost side of the limit slot 13 when sliding upward with the fixed block 18. At this time, the gear 20 and the rack 23 are in the same plane, that is, the gear 20 will rotate 180 degrees when passing the rack 23. When the limit pin enters the right-angled trapezoidal slot, it will move upward along its hypotenuse and to the right at the same time, until the limit pin enters the straight slot and the limit pin is moved to the far right. At this time, the rack 23 and the gear 20 are offset from each other in the plane so that the two are completely out of mesh. The sliding plate 16 can be driven to slide back and forth horizontally by adjusting the position of the limit pin to complete the above-mentioned adjustment of the sliding plate 16.

[0046] In this way, by moving the limit pin in the limit groove 13, the cell screen 10 can be flipped 180 degrees when it rises and the gear 20 can be disengaged from the rack 23 when the cell screen 10 descends, thereby completing the rising, flipping and descending of the cell screen 10, avoiding external structures from interfering with the sterile environment in the inner cavity 11, causing the cell suspension to be contaminated by external bacterial colonies when passing through the cell screen 10.

[0047] Furthermore, the top surfaces of the two fixed blocks 18 are fixedly installed with limit frames 30, and the sliding frames 24 are slidably installed in the sliding grooves opened in the two limit frames 30. A connecting tube 6 is fixedly installed at the center of the sliding frame 24, and an elastic bag 5 is fixedly installed on the top of the connecting tube 6. The top of the elastic bag 5 is connected to the measuring cup on the top surface of the filter 2 through a hose 4. The connecting tube 6 consists of a hard tube in the upper section and a soft tube in the lower section. The outer wall of the soft tube in the lower section of the connecting tube 6 is provided with an extrusion part for adjusting the flow rate of the liquid in the connecting tube 6.

[0048] According to the above embodiment, when the external structure detects that the activity or integrity of the cells filtered through the cell sieve 10 is lower than the preset value, the outer wall of the soft tube at the lower section of the connecting tube 6 is squeezed by the squeezing part to reduce the rate at which the cell suspension passes into the cell sieve 10, thereby reducing the friction between the cells and the cell sieve 10 to improve the cell activity and integrity. Since the elastic bag 5 is fixedly installed on the top of the connecting tube 6, and the elastic bag 5 is connected to the outside through a one-way air outlet valve, the elastic bag 5 can increase the internal capacity by discharging the internal gas, thereby maintaining the speed at which the cell suspension in the measuring cup passes through the hose 4, and avoiding the cell suspension from being contaminated by the external environment due to staying in the measuring cup for too long.

[0049] In this way, by detecting the activity and integrity of the cell suspension after passing through the cell sieve 10 in real time, the filtration speed of the cell suspension by the cell sieve 10 can be adjusted through the extrusion part, avoiding the medium with a certain viscosity from pulling down or tearing the single cells that have not passed through the cell sieve 10 under the action of its higher surface tension due to too fast filtration speed, further improving the protection ability of the device for the activity and integrity of single cells, so as to improve the preparation quality of the single cell suspension.

[0050] Furthermore, the extrusion part includes a sliding block 7 installed on the outer wall of the rigid pipe in the upper section of the connecting pipe 6 and capable of vertical sliding adjustment. Two fixing frames 8 are fixedly installed on the top surface of the sliding frame 24. Rotating rollers 9 are installed at one end of the two fixing frames 8 close to each other, and the main shaft of the roller 9 can slide along the inclined groove opened on the side wall of the fixing frame 8. Oblique grooves for the main shaft of the roller 9 to slide are also opened in the cutting grooves on both sides of the sliding block 7.

[0051] According to the above embodiments, a specific embodiment of the extrusion part is provided. When the external detection mechanism detects that the activity or integrity of the cells filtered through the cell sieve 10 is lower than the preset value, at this time, the sliding block 7 can be driven along the outer wall of the rigid pipe in the upper section of the connecting pipe 6 by the reciprocating lead screw or the micro cylinder. Specifically, refer to Figure 5 , when the sliding block 7 moves upward, the main shafts of the two side rollers 9 will be driven to move away from each other along the inclined grooves through the inclined grooves in the cutting grooves on both sides of the sliding block 7, so as to loosen the extrusion on the outer wall of the soft pipe in the lower section of the connecting pipe 6 and increase the dripping speed of the cell suspension. At this time, the main shaft of the roller 9 slides along the inclined groove opened on the side wall of the fixing frame 8 to maintain the support of the fixing frame 8 for the roller 9. When the sliding block 7 moves downward, the main shafts of the two side rollers 9 will be driven to move closer to each other along the inclined grooves through the inclined grooves in the cutting grooves on both sides of the sliding block 7, so that the two side rollers 9 can reduce the cross-section of the connecting pipe 6 by squeezing the outer wall of the soft pipe in the lower section of the connecting pipe 6, thereby reducing the dripping speed of the cell suspension.

[0052] Furthermore, a rotating wheel 28 is rotatably installed in the square groove opened on the top surface of the fixed block 18. A fixed pulley 26 is rotatably installed on the top wall of the side cavity 12. A movable pulley 27 is rotatably installed on one side of the sliding frame 24. Two pull ropes 25 are fixed on the top wall of the side cavity 12. One end of one pull rope 25 is connected to the inner bottom wall of the side cavity 12 through the movable pulley 27 and the fixed pulley 26 with a tension spring connected therebetween. The other pull rope 25 is fixedly connected to the axle bracket of the movable pulley 27 after passing around the rotating wheel 28.

[0053] According to the above embodiments, it can be known that when the fixed block 18 drives the limit frame 30 to move upward together, specifically refer to Figure 10, at this time, the rotating wheel 28 starts to rise, causing the downward pulling forces on the pulling ropes 25 on both sides of the movable pulley 27 to become unbalanced. Under the action of the tension spring, the pulling ropes 25 wound around the outer wall of the fixed pulley 26 start to tighten, driving the movable pulley 27 to start rising relative to the rotating wheel 28. Since the length of the pulling rope 25 released when the rotating wheel 28 rises is only half of the length of the pulling rope 25 released when the movable pulley 27 rises, under the action of the tension spring, the movable pulley 27 will rise at twice the speed of the rotating wheel 28, enabling the sliding frame 24 to quickly widen the distance from the fixed block 18 when rising together with the fixed block 18, leaving corresponding space for the subsequent flipping of the cell sieve 10.

[0054] Further, a flow cytometer 3 capable of monitoring cell viability is fixedly installed on the top surface of the mounting frame 1 below the filter 2. The drainer 29 is funnel-shaped and can introduce the cell suspension passed through the cell sieve 10 into the flow cytometer 3.

[0055] According to the above embodiments, when the single-cell suspension passed through the cell sieve 10 converges through the funnel-shaped drainer 29 and is introduced into the flow cytometer 3, the flow cytometer 3 can detect the single cells flowing through the optical path, thereby obtaining the viability or integrity of each single cell, completing the implementation detection of the viability or integrity of the single cells during the above filtration process, and adjusting the flow rate of the cell suspension in the connecting pipe 6 in real time according to the detection result through the electrical signal connection with the reciprocating lead screw or the micro cylinder, thereby adjusting the filtration speed of the cell suspension in real time.

[0056] Further, two concave holes are formed on the top surface of the cross plate of the sliding plate 16, and elastic pins 17 are slidably installed in the concave holes. A groove for the elastic pins 17 to insert into is provided on the bottom surface of the fixed block 18.

[0057] According to the above embodiments, when the sliding plate 16 slides horizontally under the drive of the limit pin, the elastic pins 17 on the top surface of the sliding plate 16 can insert into or disengage from the groove on the bottom surface of the fixed block 18, enabling the sliding plate 16 to be locked with the fixed block 18 when not sliding horizontally, avoiding misalignment and subsequent re-engagement of the gear 20 and the rack 23 under the influence of vibration, and preventing the cell sieve 10 from over-flipping and unable to complete the subsequent double-sided infiltration.

[0058] Further, a slider 15 is slidably installed on the bottom surface of the sliding plate 16. A cylinder 14 is fixedly installed on the bottom surface of the side cavity 12, and the end of the main shaft of the cylinder 14 is fixedly connected to the slider 15.

[0059] According to the above embodiments, when the main shaft of the cylinder 14 pushes the slider 15 to rise together with the sliding plate 16, since the sliding plate 16 will slide horizontally relative to the cylinder 14, at this time, the displacement difference between the cylinder 14 and the sliding plate 16 can be eliminated by the slider 15 sliding on the bottom surface of the sliding plate 16.

[0060] Further, the inner ring wall of the mounting ring 19 is higher than the top surface of the cell sieve 10.

[0061] According to the above-described embodiments, since the inner ring wall of the mounting ring 19 is higher than the top surface of the cell sieve 10, a reservoir can be formed between the bottom surface of the cell sieve 10 and the inner ring of the mounting ring 19. When the liquid dropping assembly wets the cell sieve 10, the immersion liquid can be made to spread over the entire surface of the cell sieve 10 by increasing the amount of the wetting liquid, thereby enhancing the wetting range of the surface of the cell sieve 10.

[0062] The standard parts used in this embodiment can be directly purchased from the market. For the non-standard structural components described in the specification and the drawings, they can also be directly processed without any doubt according to the existing technical knowledge. At the same time, the connection methods of each component adopt the mature conventional means in the existing technology, and the machines, parts and equipment all adopt the conventional models in the existing technology. Therefore, no specific description will be made here.

[0063] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell separation filter, comprising a mounting frame (1), characterized in that: A filter (2) is fixedly mounted in the mounting frame (1), an inner cavity (11) is provided in the filter (2), a cell screen (10) is slidably mounted on the side wall of the inner cavity (11), and a flow guide (29) is fixedly mounted on the side wall of the inner cavity (11) below the cell screen (10); It also includes a flip assembly, which is used to drive the cell screen (10) to slide vertically back and forth and flip 180 degrees at the same time; It also comprises a liquid dripping assembly, which is used to drip an infiltration liquid onto the surface of the cell sieve (10), and then to pass a cell suspension onto the surface of the cell sieve (10).

2. The cell separation filter according to claim 1, characterized in that: The flip assembly comprises two side chambers (12) opened on both sides of the inner chamber (11), the inner walls of the two side chambers (12) are both installed with fixed blocks (18) capable of vertical reciprocating sliding adjustment, the two fixed blocks (18) are both penetrated by a rotating shaft (22) rotatably installed, a mounting ring (19) is commonly fixed between the two rotating shafts (22), the cell screen (10) is fixedly installed on the inner ring of the mounting ring (19), the side walls of the two fixed blocks (18) are both horizontally slidably installed with a sliding plate (16) of L-shaped design, the outer wall of the sliding plate (16) is rotatably installed with a sliding shaft (21) coaxially arranged with the rotating shaft (22), the ends of the rotating shaft (22) and the sliding shaft (21) opposite to each other are plug-fitted with each other through a flat key, the end of the sliding shaft (21) away from the rotating shaft (22) is fixed with a gear (20), and the inner wall of the side chamber (12) is fixed with a rack (23); The flip assembly also includes an adjusting member for controlling the horizontal movement and adjustment of the sliding plate (16), and when the sliding plate (16) moves, the planes where the gear (20) and the rack (23) are located can be overlapped or staggered.

3. The cell separation filter according to claim 2, characterized in that: The adjusting member comprises a limiting groove (13) formed on the inner wall of the side cavity (12); the limiting groove (13) is formed by splicing a square groove, a right-angled trapezoidal groove and a straight groove and is centrally symmetrical about the midpoint of the square groove; the upper bottom edge of the right-angled trapezoidal groove is half of the lower bottom edge and is equal to the width of the straight groove; the sliding plate (16) is fixedly mounted with a limiting pin capable of sliding in the limiting groove (13); and the diameter of the limiting pin is equal to the width of the straight groove, the rack (23) and the gear (20).

4. The cell separation filter according to claim 3, characterized in that: The top surfaces of the two fixed blocks (18) are fixedly mounted with limit frames (30), and the sliding frames (24) are slidably mounted in the sliding grooves provided in the two limit frames (30). A connecting tube (6) is fixedly mounted at the center of the sliding frame (24), and an elastic bag (5) is fixedly mounted on the top of the connecting tube (6). The top of the elastic bag (5) is connected to the measuring cup on the top surface of the filter (2) through a hose (4). The connecting tube (6) is composed of a hard tube in the upper section and a soft tube in the lower section. The outer wall of the soft tube in the lower section of the connecting tube (6) is provided with an extrusion portion for adjusting the flow rate of the liquid in the connecting tube (6).

5. The cell separation filter according to claim 4, characterized in that: The extrusion part comprises a sliding block (7) which is mounted on the outer wall of the hard tube at the upper section of the connecting tube (6) and can slide vertically and adjustably. Two sets of fixed frames (8) are fixedly mounted on the top surface of the sliding frame (24). Rollers (9) are rotatably mounted on the ends of the two fixed frames (8) close to each other, and the main shaft of the roller (9) can slide along the oblique groove opened on the side wall of the fixed frame (8). The grooves opened on both sides of the sliding block (7) are also provided with oblique grooves for the main shaft of the roller (9) to slide.

6. The cell separation filter according to claim 5, characterized in that: A rotating wheel (28) is rotatably mounted in a square groove formed on the top surface of the fixed block (18); a fixed pulley (26) is rotatably mounted on the inner top wall of the side cavity (12); a movable pulley (27) is rotatably mounted on one side of the sliding frame (24); two pull ropes (25) are fixed to the inner top wall of the side cavity (12); one end of one of the pull ropes (25) passes through the movable pulley (27) and the fixed pulley (26) in sequence and is connected to a tension spring between the inner bottom wall of the side cavity (12); and the other pull rope (25) passes through the rotating wheel (28) and is fixedly connected to the shaft frame of the movable pulley (27).

7. The cell separation filter according to claim 1, characterized in that: A flow cytometer (3) capable of monitoring cell activity is fixedly mounted on the top surface of the mounting frame (1) below the filter (2), and the flow guide (29) is configured in a funnel shape and is capable of passing the cell suspension after passing through the cell sieve (10) into the flow cytometer (3).

8. The cell separation filter according to claim 2, characterized in that: The top surface of the horizontal plate of the sliding plate (16) is provided with two concave holes, and elastic pins (17) are slidably installed in the concave holes. The bottom surface of the fixed block (18) is provided with grooves for the elastic pins (17) to be inserted.

9. The cell separation filter according to claim 2, characterized in that: A slider (15) is slidably mounted on the bottom surface of the sliding plate (16), a cylinder (14) is fixedly mounted on the bottom surface of the side cavity (12), and the main shaft end of the cylinder (14) is fixedly connected to the slider (15).

10. The cell separation filter according to claim 2, characterized in that: The inner ring wall of the mounting ring (19) is higher than the top surface of the cell screen (10).

Images