Digital PCR (Polymerase Chain Reaction) equipment

Abstract

The invention relates to digital PCR (Polymerase Chain Reaction) equipment which comprises a liquid drop generation system and an optical detection system, the liquid drop generation system comprises an oil liquid supply unit, an oil liquid pumping unit, a liquid drop generation gun head and a liquid drop generation unit, wherein the oil liquid pumping unit is used for obtaining oil liquid from the oil liquid supply unit and pumping the oil liquid; the liquid drop generation gun head is used for obtaining the oil liquid pumped by the oil liquid pumping unit and can suck a sample and output liquid drops; the oil liquid supply unit comprises an oil liquid container with an oil liquid accommodating space and a liquid outlet part, an oil nozzle which can be driven to move along a preset path and is provided with a first position and a second position, and a locking device for keeping the oil nozzle at the first position; the optical detection system comprises a light source, a camera and a light filtering unit. The light filtering unit comprises a transmitting end light filter wheel, a receiving end light filter wheel, a belt wheel transmission mechanism and a switching driving motor. The operation performance of the digital PCR equipment can be improved, and the detection efficiency and the detection operation stability are improved.

Description

Technical Field

[0001] The present invention relates to a digital PCR device, and particularly to a droplet generation system and an optical detection system in the device. Background Art

[0002] A digital PCR device mainly consists of a droplet generation system, an optical detection system, a thermal cycling system, etc. Among them, the droplet generation system for microdropletizing a sample usually includes an oil supply unit for providing oil (formulation oil), an oil pumping unit for obtaining oil from the oil supply unit and pumping the oil, a droplet generation tip for obtaining the oil pumped by the oil pumping unit and capable of sucking the sample to be detected and outputting droplets, and a droplet generation unit for forming droplets by vibrating the liquid in the droplet generation tip. The oil supply unit and the oil pumping unit, and the oil pumping unit and the droplet generation tip are respectively connected by pipelines. The oil pumping unit first sucks oil from the oil supply unit and then pumps it into the droplet generation tip to discharge the gas therein. After the droplet generation tip sucks the sample to be detected, microdroplets are formed and discharged through the droplet generation unit.

[0003] Limited by the structural design of each module, the current digital PCR equipment still needs to be improved in many aspects such as operation, efficiency, and stability of droplet generation. For example, in the existing droplet generation system, the oil supply unit generally includes a container with a liquid accommodating space, a cover body that can be detached relative to the container, and an infusion tube. The infusion tube runs from the inside of the container to the outside of the container and is used to connect the back-end mechanism outside the container. With this structural setting, when disassembling the container to replace it, it is necessary to open the cover body and extract the infusion tube from the container. In this process, the extracted infusion tube will carry part of the liquid out of the container and cause the liquid to spill and contaminate the working area. At the same time, the installation of this structure requires the insertion of the infusion tube into the container, which is difficult to achieve quick and convenient assembly; in addition, the oil pumping unit often uses a screw nut transmission mechanism to drive the plunger pump to slowly push out the oil in the plunger pump. The liquid pumping mechanism of this structure is subject to mechanical vibration, especially the flow fluctuation caused by the gap vibration of the screw, which cannot be solved, because the driving force on the nut when the screw drives the nut to move is not uniform, but changes periodically. Therefore, this structure is difficult to achieve liquid output with stable flow, and cannot meet the demand for precise flow output, which is not conducive to obtaining stable and uniform droplets. In addition, the optical detection system includes multiple transmitting end filters and multiple receiving end filters. When performing optical detection, it is required that the transmitting end filter and the receiving end filter have a unique corresponding relationship, so it is required that the transmitting end filter and the receiving end filter can be synchronously switched to their respective use positions, while the filters in the existing digital PCR equipment can only be switched manually, and the transmitting end filter and the receiving end filter need to be switched separately, which is time-consuming and labor-intensive and difficult to achieve accurate synchronous switching. In addition, when the generated droplets fall into the container of the consumables, it is necessary to temporarily transfer and place the cover plate of the consumables, which can only be achieved through manual operation at present, which reduces efficiency and easily causes droplet contamination. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide an improved digital PCR device with better operability, higher efficiency and better stability.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is: A digital PCR device, comprising: A droplet generation system, the droplet generation system comprising an oil supply unit for providing oil, an oil pumping unit for obtaining oil from the oil supply unit and pumping the oil, a droplet generation gun head for obtaining the oil pumped by the oil pumping unit and capable of sucking samples and outputting droplets, and a droplet generation unit; The oil supply unit includes an oil container having an oil accommodation space and a liquid outlet portion, a nozzle that can be driven to move along a preset path and has a first position and a second position, and a locking device for keeping the nozzle in the first position. The nozzle has an internal passage. The oil supply unit has a first state and a second state. When in the first state, the nozzle is in the first position, the nozzle is tightly connected to the oil container, and the internal passage of the nozzle communicates with the liquid outlet portion of the oil container; when in the second state, the nozzle is in the second position, the nozzle is separated from the oil container, and the internal passage of the nozzle is disconnected from the liquid outlet portion of the oil container. An optical detection system, the optical detection system includes a light source, a camera, and a filter unit. The filter unit includes a transmitting end filter wheel corresponding to the light source, a receiving end filter wheel corresponding to the camera, a belt drive mechanism, and a switching drive motor. The belt drive mechanism is used to drive the transmitting end filter wheel, the receiving end filter wheel, and the switching drive motor in the same phase. When the switching drive motor works, the belt drive mechanism drives the transmitting end filter wheel and the receiving end filter wheel to rotate synchronously and in the same phase to correspondingly switch the filter.

[0006] According to a specific embodiment of the present invention, the belt drive mechanism includes a synchronous belt, a main drive synchronous pulley coaxially connected to the output shaft of the drive motor, an active gear coaxially connected to the main drive synchronous pulley, a driven synchronous pulley, and a first driven gear coaxially connected to the driven synchronous pulley. The synchronous belt is wound between the main drive synchronous pulley and the driven synchronous pulley. The active gear is in transmission connection with the receiving end filter wheel, and the first driven gear is in transmission connection with the transmitting end filter wheel.

[0007] Further, the belt drive mechanism further includes a second driven gear. The second driven gear is coaxially connected to the receiving end filter wheel and is in meshing transmission with the active gear. The circumferential surface of the transmitting end filter wheel is provided with teeth, and the first driven gear is in meshing transmission with the teeth of the transmitting end filter wheel.

[0008] Preferably, the belt drive mechanism further includes a plurality of tension wheels for keeping the synchronous belt in a tensioned state.

[0009] According to a specific embodiment of the present invention, two driven synchronous pulleys are symmetrically arranged on both sides of the main drive synchronous pulley, and the two driven synchronous pulleys are in one-to-one transmission connection with the two transmitting end filter wheels.

[0010] According to a specific embodiment of the present invention, the oil supply unit further includes a driving device for driving the nozzle to move along the preset path between the first position and the second position; the driving device includes a rotatably arranged driving member and a trigger ring formed on the outer periphery of the nozzle. When the driving member rotates, it drives the nozzle to move linearly by abutting against the trigger ring.

[0011] Preferably, the oil supply unit includes a support seat, the support seat has a first mounting portion, the nozzle is arranged on the first mounting portion, the driving member has a driving arm and a connecting portion rotatably connected to the first mounting portion, and the trigger ring includes an upper trigger ring and a lower trigger ring which are arranged at intervals and are respectively located on both sides of the driving arm.

[0012] According to a preferred embodiment of the present invention, the driving device further includes an operating handle connected to the driving member, and the locking device includes a clamping groove arranged on the oil container and a clamping head formed at one end of the operating handle. When in the first state, the clamping head is embedded in the clamping groove.

[0013] Preferably, the liquid outlet portion includes a liquid outlet hole and a sealing ring arranged on the hole wall of the liquid outlet hole; the nozzle includes a liquid pipe having the internal channel, the liquid pipe is opposite to the sealing ring, and one end of the liquid pipe facing the sealing ring has an inclined surface; the end surface of the sealing ring facing the liquid pipe also has an inclined surface; when in the first state, the inclined surface of the liquid pipe is pressed against the inclined surface of the sealing ring.

[0014] Furthermore, an installation hole is formed on the oil container, an oil container cover is fixedly arranged in the installation hole, the liquid outlet hole is formed on the oil container cover, and a ventilation hole is also formed on the oil container cover; and / or, a hole wall located outside the oil container is arranged at the edge of the installation hole, and a convex edge abutted against the end surface of the hole wall is arranged on the outer periphery of the oil container cover.

[0015] Preferably, the oil supply unit further includes a guide seat for guiding the movement of the nozzle along the preset path, and the guide seat surrounds the nozzle.

[0016] According to a specific and preferred embodiment of the present invention, the oil pumping unit includes a piston pump, a pumping motor, a lead screw-nut transmission mechanism connecting the pumping motor and the piston pump, and an elastic structure. The piston pump includes a pump body and a piston slidably connected to the pump body. The lead screw-nut transmission mechanism includes a pumping lead screw, a pumping nut, and a transmission seat. The pumping lead screw is connected to the pumping motor, the pumping nut is connected to the transmission seat, and the transmission seat is connected to the piston of the piston pump. The lead screw-nut transmission mechanism drives the piston to move in the first direction under the drive of the pumping motor to achieve liquid pumping. The elastic structure is used to provide a thrust force opposite to the first direction to the piston when the piston moves in the first direction. In a more specific embodiment, the pump body of the piston pump is connected to the first port of a three-way valve. The second port of the three-way valve is communicated with the nozzle, and the third port of the three-way valve is communicated with the droplet generating nozzle tip.

[0017] Preferably, the elastic structure includes an elastic member, and the axial extension direction of the elastic member is the same as the first direction; the elastic member is sleeved on the outer periphery of the pumping lead screw, or the elastic member includes a first elastic member and a second elastic member symmetrically distributed on both sides of the pumping lead screw; when the piston moves in the first direction, the elastic force generated by the compression of the elastic member forms the thrust force, and the elastic member is arranged such that the thrust force formed by its compression is always less than the driving force of the lead screw-nut transmission mechanism on the piston.

[0018] In a preferred and specific embodiment, the elastic member is a spring.

[0019] According to a preferred embodiment of the present invention, the oil pumping unit includes a vertically extending mounting plate and a support plate horizontally arranged on the mounting plate. A pair of connecting seats are arranged on the mounting plate. The pumping lead screw is vertically arranged and its two ends are respectively rotatably connected to the pair of connecting seats. The pumping nut is in threaded engagement with the pumping lead screw. The transmission seat is connected to the mounting plate. The elastic structure is vertically arranged, and the two end portions of the elastic structure are respectively connected to the support plate and the transmission seat; the transmission seat penetrates through the mounting plate. The elastic structure, the pumping lead screw, and the pumping nut are located on one side of the mounting plate, and the piston pump is located on the other side of the mounting plate opposite to the one side. By adopting this oil pumping unit, by adding an elastic structure, which is used to provide a thrust force opposite to the first direction to the piston when the piston moves in the first direction, the pumping nut is always pressed tightly in the thread groove on the pumping lead screw, and the pumping nut and the transmission seat always receive a stable driving force, effectively eliminating the clearance vibration generated during the driving process of the pumping lead screw, thereby realizing the stable liquid output of the flow rate and improving the stability of the detection operation.

[0020] Preferably, the oil pumping unit further includes a guiding mechanism, which includes a pumping guide rail disposed on one of the driving seat and the mounting plate, and a pumping guide groove disposed on the other of the driving seat and the mounting plate, and the pumping guide rail is slidably connected to the pumping guide groove.

[0021] According to a specific embodiment of the present invention, the digital PCR device includes a box body, a moving detection platform movably disposed on the box body, a moving carrying platform movably disposed in the box body, an X-axis moving mechanism, an X-axis driving mechanism, a Y-axis moving mechanism, and a Y-axis driving mechanism; the droplet generation system and the optical detection system are both disposed on the moving detection platform, the X-axis moving mechanism is connected between the box body and the X-axis driving mechanism, and when the X-axis driving mechanism works, the moving detection platform is driven to translate along the X-axis direction through the X-axis moving mechanism, the Y-axis moving mechanism is connected between the box body and the Y-axis driving mechanism, and when the Y-axis driving mechanism works, the moving carrying platform is driven to translate along the Y-axis direction through the Y-axis moving mechanism.

[0022] As a preferred embodiment of the present invention, the X-axis moving mechanism includes an X-axis synchronous belt driving along the X-axis direction, a plurality of synchronous wheels, a connecting member respectively connected to the moving detection platform and the X-axis synchronous belt, an X-axis guide rail parallel to the X-axis direction disposed on one of the box body and the moving detection platform, and an X-axis guide groove disposed on the other of the box body and the moving detection platform; the X-axis synchronous belt is wound around each of the synchronous wheels, and at least one of the synchronous wheels is in transmission connection with the X-axis driving mechanism; the X-axis guide rail is slidably connected to the X-axis guide groove; a plurality of X-axis sliders are disposed at the bottom of the moving detection platform, and the X-axis guide rail or the X-axis guide groove is disposed on the X-axis sliders.

[0023] As a preferred embodiment of the present invention, the Y-axis moving mechanism includes a Y-axis driving lead screw axially disposed along the Y-axis direction, a Y-axis moving nut threadedly connected to the Y-axis driving lead screw, a Y-axis guide rail parallel to the Y-axis direction disposed on one of the box body and the moving carrying platform, and a Y-axis guide groove disposed on the other of the box body and the moving carrying platform, the moving carrying platform is connected to the Y-axis moving nut, and the Y-axis guide rail is slidably connected to the Y-axis guide groove; a plurality of Y-axis sliders are disposed at the bottom of the moving carrying platform, and the Y-axis guide rail or the Y-axis guide groove is disposed on the Y-axis sliders.

[0024] According to a preferred embodiment of the present invention, the digital PCR device further includes a plurality of thermal cycling systems disposed on the mobile carrying platform. The thermal cycling system includes a placement groove for placing consumables and a heating unit for performing high and low temperature cycling heating on the consumables. Preferably, there is a starting position inside the box, a bottom plate is provided at the starting position, and a first position corresponding to the droplet generation system is provided on the bottom plate. A plurality of waste liquid grooves are provided at the first position. The consumables include an amplification container and a cover plate. The digital PCR device further includes a first transfer mechanism for moving the cover plate of the consumables from the placement groove to the first position on the bottom plate. The first transfer mechanism is disposed at the bottom of the mobile detection platform. A plurality of magnetic metal sheets are provided on the cover plate. The first transfer mechanism includes a plurality of magnetic attachments embedded in the bottom of the mobile detection platform and corresponding one by one to the magnetic metal sheets on the cover plate. In a preferred and specific embodiment, the magnetic attachment is an electromagnet.

[0025] Further preferably, a second position is further provided on the bottom plate at the side of the first position. The digital PCR device further includes a second transfer mechanism for transferring the cover plate of the consumables from the first position to the second position. Even more preferably, the second transfer mechanism includes a carrier plate slidably disposed on the bottom plate for carrying the cover plate, a push plate disposed at the edge of the carrier plate, a translation driving member disposed at the side of the mobile carrying platform and capable of contacting the push plate, a translation guide rail disposed on one of the bottom plate and the carrier plate, and a translation guide groove disposed on the other of the bottom plate and the carrier plate. The translation guide rail is slidably connected to the translation guide groove. When the translation driving member moves along the Y-axis direction with the mobile carrying platform, the translation driving member drives the carrier plate to move along the Y-axis direction through the push plate. Through the setting of the transfer mechanism, the temporary transfer and placement of the consumable cover plate can be automatically realized, which not only improves the efficiency but also avoids and reduces droplet contamination.

[0026] Preferably, a hanging hole is provided on the push plate, and the translation driving member has a hanging rod that can extend or retract. When the hanging rod extends, it is inserted into the hanging hole.

[0027] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art: The present invention can improve the operating performance of the digital PCR device, improve the detection efficiency and the stability of the detection operation. Among them, the improved oil supply unit can not only facilitate the disassembly and installation of the liquid-providing container during the container replacement process, but also avoid problems such as liquid spillage during disassembly and replacement, which helps to ensure the smooth progress of the detection and promote the improvement of work efficiency; the improved optical detection system adopts a pulley drive mechanism based on a synchronous belt, which can conveniently and accurately synchronously switch the emission-end filter and the receiving-end filter, ensuring the unique correspondence of the emission-end filter and the receiving-end filter during use and improving the efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] FIG. Figure 1 is a schematic structural view of the digital PCR device of the present invention when the mobile detection platform is located on the left side of the base.

[0029] FIG. Figure 2 is a schematic structural view of the digital PCR device of the present invention when the mobile detection platform moves to the middle of the base.

[0030] FIG. Figure 3 is a front view schematic of the oil supply unit in the digital PCR device of the present invention.

[0031] FIG. Figure 4 is a sectional view taken along line A-A of the oil supply unit in the digital PCR device of the present invention in the first state.

[0032] FIG. Figure 5 is an enlarged schematic view of part B of the oil supply unit in the digital PCR device of the present invention.

[0033] FIG. Figure 6 is a sectional view taken along line A-A of the oil supply unit in the digital PCR device of the present invention in the second state.

[0034] FIG. Figure 7 is an enlarged schematic view of part C of the oil supply unit in the digital PCR device of the present invention.

[0035] FIG. Figure 8 is a partial structural schematic view of the optical detection system in the digital PCR device of the present invention from the first perspective.

[0036] FIG. Figure 9 is a partial structural schematic view of the optical detection system in the digital PCR device of the present invention from the second perspective.

[0037] FIG. Figure 10 is a three-dimensional schematic view of the oil pumping unit in the digital PCR device of the present invention from the first perspective.

[0038] FIG.Figure 11 This is a perspective schematic diagram of the second view of the oil pumping unit in the digital PCR device of the present invention.

[0039] In the above drawings: 1. Box body; 2. Mobile detection platform; 3. Mobile bearing platform; 4. X-axis moving mechanism; 5. X-axis driving mechanism; 8. Droplet generation system; 9. Optical detection system; 10. Thermal cycling system; 11. X-axis synchronous belt; 12. Synchronous pulley; 14. X-axis guide rail; 15. X-axis slider; 16. Y-axis driving lead screw; 17. Y-axis guide rail; 19. Oil container; 20. Oil nozzle; 21. Support; 22. First mounting part; 23. Second mounting part; 24. Conduit; 25. Liquid outlet part; 26. Driving part; 27. Upper trigger ring; 28. Lower trigger ring; 29. Operating handle; 30. Clamping groove; 31. Clamping head; 32. Sealing ring; 33. Liquid outlet hole; 34. Container cover; 35. Guide seat; 36. Plunger pump; 37. Pumping motor; 38. Pumping lead screw; 39. Pumping nut; 40. Transmission seat; 41. Base; 42. First spring; 43. Second spring; 44. Mounting plate; 45. Support plate; 46. Connecting seat; 47. Pumping guide rail; 48. Pumping guide groove; 49. Valve plate; 50. Light source; 51. Camera; 52. Filter wheel at the emission end; 53. Filter wheel at the receiving end; 54. Switching drive motor; 55. Synchronous belt; 56. Main drive synchronous pulley; 57. Driven synchronous pulley; 58. First driven gear; 59. Second driven gear; 60. Tensioning pulley; 61. Base plate; 62. Waste liquid tank; 64. Amplification container; 65. Cover plate; 66. Magnetic metal sheet; 67. Carrier plate; 68. Pushing plate; 69. Translation drive part; 70. Translation guide rail; 71. Driving gear. Detailed implementation manners

[0040] In order to enable those skilled in the art of the present technology to better understand the solution of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0041] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. These are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0042] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "mounted", "connected", "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0043] As shown in the attached Figure 1 and the attached Figure 2 The digital PCR device shown in the figure includes a box body 1, a moving detection platform 2, a moving bearing platform 3, an X-axis moving mechanism 4, an X-axis driving mechanism 5, a Y-axis moving mechanism, a Y-axis driving mechanism, a droplet generation system 8, an optical detection system 9, and a plurality of thermal cycling systems 10. The moving detection platform 2 is movably arranged on the box body 1, the moving bearing platform 3 is movably arranged inside the box body 1, the droplet generation system 8 and the optical detection system 9 are both arranged on the moving detection platform 2, and the thermal cycling system 10 is arranged on the moving bearing platform 3.

[0044] The X-axis moving mechanism 4 is connected between the box body 1 and the X-axis driving mechanism 5. The X-axis moving mechanism 4 includes an X-axis synchronous belt 11 that drives in the X-axis direction, a plurality of synchronous wheels 12, and a connecting member that is respectively connected to the moving detection platform 2 and the X-axis synchronous belt 11. The X-axis synchronous belt 11 is wound around each synchronous wheel 12, and at least one synchronous wheel 12 is in transmission connection with the X-axis driving mechanism 5. When the X-axis driving mechanism 5 works, it drives the moving detection platform 2 to translate in the X-axis direction through the X-axis moving mechanism 4. Further, the X-axis moving mechanism 4 further includes an X-axis guide rail 14 parallel to the X-axis direction arranged on one of the box body 1 and the moving detection platform 2 and an X-axis guide groove arranged on the other of the box body 1 and the moving detection platform 2. The X-axis guide rail 14 is slidably connected to the X-axis guide groove. A plurality of X-axis sliders 15 can be arranged at the bottom of the moving detection platform 2, and the X-axis guide rail 14 or the X-axis guide groove is arranged on the X-axis sliders 15.

[0045] The Y - axis moving mechanism is connected between the box body 1 and the Y - axis driving mechanism. The Y - axis moving mechanism includes a Y - axis driving lead screw 16 arranged axially along the Y - axis direction, a Y - axis moving nut threadedly connected to the Y - axis driving lead screw 16, and the moving bearing platform 3 is connected to the Y - axis moving nut. When the Y - axis driving mechanism works, it drives the moving bearing platform 3 to translate along the Y - axis direction through the Y - axis moving mechanism. Further, the Y - axis moving mechanism also includes a Y - axis guide rail 17 parallel to the Y - axis direction arranged on one of the box body 1 and the moving bearing platform 3, and a Y - axis guide groove arranged on the other of the box body 1 and the moving bearing platform 3. The Y - axis guide rail 17 is slidably connected to the Y - axis guide groove. Multiple Y - axis sliders can be arranged at the bottom of the moving bearing platform 3, and the Y - axis guide rail 17 or the Y - axis guide groove is arranged on the Y - axis sliders.

[0046] The droplet generation system 8 for micro - dropletizing a sample includes an oil supply unit for providing oil, an oil pumping unit for obtaining oil from the oil supply unit and pumping the oil, a droplet generation tip for obtaining the oil pumped by the oil pumping unit and capable of sucking the sample and outputting droplets, and a droplet generation unit. They are arranged in sequence on the moving detection platform 2. The oil supply unit and the oil pumping unit, and the oil pumping unit and the droplet generation tip are respectively connected by pipelines. Then, the oil pumping unit first sucks oil from the oil supply unit and then slowly pumps it into the droplet generation tip to discharge the gas therein. After the droplet generation tip sucks the sample to be detected, micro - droplets are formed and discharged by means of vibration generated by the droplet generation unit.

[0047] As shown in the appendix Figure 3 to the appendix Figure 7 As shown, the oil supply unit includes an oil container 19, which has a liquid accommodation space and a liquid outlet 25. The required liquid, such as oil, is contained in the liquid accommodation space. The liquid outlet 25 communicates the inside and outside of the oil container 19, and the liquid in the oil container 19 can be led out of the oil container 19 through the liquid outlet 25. The oil supply unit also includes an oil nozzle 20, which has an internal channel. The oil nozzle 20 is movably arranged outside the oil container 19, and it can be driven to move along a preset path. Moreover, the oil nozzle 20 also has a first position and a second position. When the oil nozzle 20 moves along the preset path, it can switch between the first position and the second position. At the same time, the oil supply unit also includes a locking device, which is used to keep the oil nozzle 20 in the first position when the oil nozzle 20 moves to the first position. The oil nozzle 20 is used to connect the rear - end liquid path system to transport oil.

[0048] The oil supply unit has the first state as shown in the appendix Figure 4 and the appendix Figure 5 as shown, and the second state as shown in the appendix Figure 6 and the appendix Figure 7The second state shown. When in the first state, the nozzle 20 is in the first position, the nozzle 20 is tightly connected to the oil container 19, and the internal passage of the nozzle 20 communicates with the liquid outlet part 25 of the oil container 19. When in the second state, the nozzle 20 is in the second position, the nozzle 20 is separated from the oil container 19, and the internal passage of the nozzle 20 is disconnected from the liquid outlet part 25 of the oil container 19.

[0049] The locking device includes a mating clamping groove 30 and a clamping head 31. The clamping groove 30 is provided on the oil container 19, and the clamping head 31 is connected to the driving device. Then when the oil supply unit is in the first state, the clamping head 31 is inserted into the clamping groove 30, and at this time the driving device cannot move freely, so as to keep the nozzle 20 in the first position by restricting the driving device.

[0050] The oil supply unit includes a support 21 for supporting and installing each component. The support 21 has a first mounting part 22, and the nozzle 20 is arranged on the first mounting part 22 and can move relative to the first mounting part 22. The first mounting part 22 can be in the shape of a plate, with a through hole opened thereon, and the nozzle 20 is passed through the through hole. The support 21 can also have a second mounting part 23 while having the above-mentioned first mounting part 22, and the second mounting part 23 is used for accommodating and / or supporting the oil container 19.

[0051] The preset path of the movement of the nozzle 20 extends in the up and down direction, that is, the nozzle 20 can move in the up and down direction. The nozzle 20 includes a liquid pipe with an internal passage, and the axial direction of the liquid pipe is arranged along the movement direction of the nozzle 20, that is, the up and down direction. When the nozzle 20 is in the first position, the nozzle 20 is located above or below the oil container 19. Correspondingly, the liquid outlet part 25 is arranged at the top or bottom of the oil container 19. In this embodiment, the scheme of the nozzle 20 being located above the oil container 19 is adopted. At this time, a conduit 24 is also arranged in the oil container 19. One end (upper end) of the conduit 24 communicates with the liquid outlet part 25 of the oil container 19, and the other end (lower end) extends to the lower half of the oil container 19, close to the bottom of the oil container 19, and is preferably arranged as an inclined end face and contacts the bottom of the oil container 19. The lower end of the nozzle 20 is used to connect the oil container 19, and the upper end is used to connect the rear liquid path system. For example, a connection bayonet is arranged at the upper end of the nozzle 20, and the connection bayonet can be connected to the rear liquid path system through a flexible hose.

[0052] The nozzle 20 can move between the first position and the second position along the preset path. To facilitate the stable and controllable movement of the nozzle 20, the oil supply unit further includes a driving device to drive the nozzle 20. The driving device can be a manual driving device, an electric driving device, or a driving device that can be both manual and electric.

[0053] In this embodiment, the driving device includes a driving member 26, a trigger ring, and an operating handle 29. The driving member 26 is movably arranged and drives the nozzle 20 to move when it moves. In this embodiment, the driving member 26 is rotatably connected to the first mounting portion 22 of the support 21 through a rotating shaft. The driving member 26 includes a connecting portion rotatably connected to the first mounting portion 22 at one end and a driving arm connected to the other end of the connecting portion. The axis of the connecting portion forms a certain angle with the axis of the driving arm. In this embodiment, the axis of the connecting portion is substantially perpendicular to the axis of the driving arm, thus forming a bent arm. The rotating shaft and the connecting portion are located on one side of the nozzle 20, and the driving arm extends from both sides of the middle of the nozzle 20 to the other side of the nozzle 20. Two parallel rib plates can be provided on the first mounting portion 22 for mounting the rotating shaft to enable the driving member 26 to be rotatably mounted. The trigger ring is arranged on the outer periphery of the nozzle 20 and can be in contact with the driving member 26, specifically in contact with the driving arm of the driving member 26. Thus, when the driving member 26 rotates, it can push the trigger ring and then drive the nozzle 20 to move. The trigger ring includes an upper trigger ring 27 arranged on one side of the driving member 26 and a lower trigger ring 28 arranged on the other side of the driving member 26. The two trigger rings are arranged at intervals and are respectively used to realize the movement of the nozzle 20 in the up and down movement directions. When the driving member 26 rotates upward, it pushes the upper trigger ring 27 from below and drives the nozzle 20 to move upward; when the driving member 26 rotates downward, it pushes the lower trigger ring 28 from above and drives the nozzle 20 to move downward. The movement of the driving member 26 can be manually driven by a person or electrically driven. In this embodiment, the driving device is a manual driving device, and it further includes an operating handle 29. The operating handle 29 is connected to the driving arm of the driving member 26, and the operation of the driving member 26 can be realized by operating the operating handle 29. One end of the operating handle 29 is fixedly connected to the driving member 26, and a protrusion for a person to apply a force can be provided in the middle of the operating handle 29. Based on the solution including the operating handle 29, the clamping joint 31 in the locking device is formed at one end of the operating handle 29. When in the first state, the operating handle 29 rotates towards the direction of the oil container 19, and the clamping joint 31 at one end of it is embedded into the clamping groove 30 on the oil container 19. The surface of the clamping groove 30 in contact with the clamping joint 31 is an inclined surface, so that the clamping joint 31 is not easily slipped out of the clamping groove 30 under the action of this inclined surface, but needs to apply a certain amount of external force to be able to slip out of the clamping groove 30 to achieve unlocking.

[0054] To ensure that the nozzle 20 moves along a preset path and reduce deviation, the detection device further includes a guide seat 35 for guiding the movement of the nozzle 20 along the preset path. The guide seat 35 is arranged around the nozzle 20 and can be installed on the first mounting portion 22.

[0055] When the driving device is an electric driving device or a driving device that can be manual or electric, the driving device further includes an electric push rod connected to the driving member 26. The two ends of the electric push rod can be respectively hinged to the first mounting portion 22 and the driving member 26. When the electric push rod extends or contracts, it can drive the driving member 26 to rotate.

[0056] The liquid outlet portion 25 of the liquid container 19 includes a liquid outlet hole 33 and a sealing ring 32, and the sealing ring 32 is arranged on the hole wall of the liquid outlet hole 33. In this embodiment, a mounting hole is formed in the liquid container 19, and a container cover 34 is fixedly arranged at the mounting hole, and the liquid outlet hole 33 is formed in the container cover 34. More specifically, a hole wall located outside the liquid container 19 is provided at the edge of the mounting hole, and a convex edge that abuts against the end face of the hole wall is provided on the outer periphery of the container cover 34, so as to realize the installation of the container cover 34 through the cooperation between the convex edge and the end face of the hole wall. The container cover 34 has a relatively large thickness, and the axial length of the liquid outlet hole 33 formed therein is greater than the axial length of the sealing ring 32, and the sealing ring 32 is fixed on the hole wall in the middle of the liquid outlet hole 33 through a stepped structure. Above the liquid outlet hole 33 and the sealing ring 32, the liquid pipe of the oil nozzle 20 has an inclined surface at the end facing the sealing ring 32 of the liquid container 19, and the end face of the sealing ring 32 facing the oil nozzle 20 also has an inclined surface. When in the first state, the inclined surface of the liquid pipe presses against the inclined surface of the sealing ring 32, causing the sealing ring 32 to undergo elastic deformation, realizing axial and circumferential sealing connection. A ventilation hole is also formed in the container cover 34 to communicate the inside and outside of the liquid container 19, and a pair of ventilation holes can be formed on the two opposite sides of the liquid outlet hole 33. For the unused liquid container 19, a sealing film covering the liquid outlet hole 33 and the ventilation hole is provided on its container cover 34 to ensure the sealing of the unused liquid container 19 during transportation and prevent liquid leakage.

[0057] The process of replacing the oil container 19 in the above-mentioned oil supply unit is as follows: Unlock the locking device, and manually or electrically rotate the driving member 26 upward. During this process, drive the nozzle 20 to move upward under the guiding action of the guiding seat 35 through the upper trigger ring 27, so that the lower end of the nozzle 20 disengages from the liquid outlet part 25 of the oil container 19. Peel off the sealing film on the container cover 34 of the unused oil container 19 and place the oil container 19 at the designated position on the support 21. Manually or electrically rotate the driving member 26 downward. During this process, drive the nozzle 20 to move downward until the limit position under the guiding action of the guiding seat 35 through the lower trigger ring 28, so that the lower end of the nozzle 20 is in close contact with the sealing ring 32 by entering the liquid outlet hole 33, realizing a tight connection with the oil container 19. At this time, the internal channel of the nozzle 20 is communicated with the liquid outlet hole 33 of the liquid outlet part 25 of the oil container 19, and the clamping head 31 at the end of the operating handle 29 is embedded in the clamping groove 30 to lock the position of the nozzle 20. At this time, the gas in the nozzle 20 and the internal conduit 24 of the oil container 19 can be emptied to the inside of the oil container 19 through the rear-end liquid path system, and then oil is injected into the rear end. The air vent hole on the oil container 19 can timely replenish the pressure in the oil container 19 to make the internal and external pressures of the oil container 19 equal. During the process of replacing the oil container 19, the liquid in the oil container 19 will not spill and pollute the detection environment, and this process is convenient to operate and takes less time.

[0058] In the above-mentioned oil supply unit, the conduit 24 that touches the bottom is built in the oil container 19, which can maximize the utilization of the liquid and reduce liquid residue and waste. The sealing ring 32 is embedded in the pore wall of the liquid outlet hole 33 on the container cover 34 or is secondarily injection-molded in the liquid outlet hole 33. Through the cooperation between the inclined surface on the sealing ring 32 and the inclined surface on the nozzle 20, the sealing connection between the nozzle 20 and the oil container 19 is realized.

[0059] Multiple sets (for example, two sets) of the above-mentioned oil supply units can be set on the same detection device to realize the supply function of different liquids or the supply and backup supply functions of the same liquid.

[0060] As shown in the attached Figure 10 and the attached Figure 11 As shown, the oil pumping unit includes a plurality of plunger pumps 36 arranged in a row, a pumping motor 37, and a lead screw-nut transmission mechanism. The lead screw-nut transmission mechanism is respectively connected to the pumping motor 37 and the plunger pump 36. The power output by the pumping motor 37 is transmitted to the plunger pump 36 through the lead screw-nut transmission mechanism to realize the driving of the plunger pump 36. The specific scheme is: The plunger pump 36 includes a cylindrical pump body and a plunger slidably connected to the pump body. The plunger is inserted into the pump body and can slide along the axial direction of the pump body. Then, when the plunger slides in different directions, it can suck the liquid into the pump body or pump the liquid in the pump body out.

[0061] The pumping motor 37 is preferably a servo pumping motor 37 or a stepper pumping motor 37, especially a stepper motor with a step angle less than or equal to 0.9 degrees.

[0062] The lead screw nut transmission mechanism includes a pumping lead screw 38, a pumping nut 38, and a transmission seat 40. The pumping lead screw 38 is coaxially connected to the output shaft of the pumping motor 37. The pumping nut 38 is in threaded engagement with the pumping lead screw 38, and the pumping nut 38 is connected to the transmission seat 40. The plunger of the plunger pump 36 is also connected to the transmission seat 40. The transmission seat 40 is symmetrically arranged with respect to the plane where the center of the pumping nut 38 is located. When the pumping lead screw 38 rotates, the pumping nut 38 and the transmission seat 40 move together along the axial direction of the pumping lead screw 38, driving the plunger to move relative to the pump body. When pumping liquid, the lead screw nut transmission mechanism drives the plunger to move in the first direction under the drive of the pumping motor 37. When sucking liquid, the lead screw nut transmission mechanism drives the plunger to move in the direction opposite to the first direction under the drive of the pumping motor 37. On this basis, the hydraulic fluid pumping unit further includes an elastic structure, which is used to provide a thrust force opposite to the first direction to the plunger, the pumping nut 38, and the transmission seat 40 when the plunger moves in the first direction. When the pumping lead screw 38 rotates, it provides a driving force in the first direction to the pumping nut 38. Under the action of this thrust force, the pumping nut 38 fits on the side chute wall of the thread of the pumping lead screw 38 that provides the driving force, so as to prevent the pumping nut 38, the transmission seat 40, and the plunger from vibrating due to the gap between the pumping nut 38 and the pumping lead screw 38, and thus the plunger can be smoothly pushed forward.

[0063] In the illustrated embodiment, the elastic structure includes an elastic member, and the axial extension direction of the elastic member is the same as the first direction. The elastic member can specifically be a spring. The elastic member can be sleeved on the outer periphery of the pumping lead screw 38, or symmetrically distributed on both sides of the pumping lead screw 38 to provide a balanced thrust force. In this embodiment, the elastic member includes a first elastic member and a second elastic member, that is, a first spring 42 and a second spring 43, which are symmetrically distributed on both sides of the axial direction of the pumping lead screw 38. The axis of the elastic member is coplanar with the axis of the pumping lead screw 38, or the axis of the elastic member is not coplanar with the axis of the pumping lead screw 38 but can provide a balanced thrust force on both sides.

[0064] When the plunger moves in the first direction, the elastic force generated by the compression of the elastic member forms a thrust force. The elastic member is set such that the thrust force formed by its compression is always less than the driving force of the lead screw nut transmission mechanism on the plunger, or in other words, the thrust force provided by the elastic member to the pumping nut 38 and the transmission seat 40 is always less than the driving force of the pumping lead screw 38 on the pumping nut 38.

[0065] The oil pumping unit further includes a mounting plate 44 and a support plate 45 for mounting various components. Specifically, the plate surface of the mounting plate 44 is arranged parallel to the first direction, the support plate 45 is arranged on the mounting plate 44 and its plate surface is perpendicular to the mounting plate 44. For example, the mounting plate 44 can be arranged to extend in the vertical direction, and the support plate 45 is horizontally arranged on the mounting plate 44. The elastic structure, the pumping lead screw 38, the pumping nut 38 and the transmission seat 40 are located on one side of the mounting plate 44, and the plunger pump 36 is located on the other side of the mounting plate 44 opposite to the one side. On the side where the pumping lead screw 38 is located, a pair of connecting seats 46 are spaced on the mounting plate 44. The pumping lead screw 38 is arranged along the direction parallel to the plate surface of the mounting plate 44, that is, vertically arranged, and both ends of the pumping lead screw 38 are rotatably connected to a pair of rotating seats. The transmission seat 40 is slidably connected to the mounting plate 44. The elastic structure is also arranged along the direction parallel to the plate surface of the mounting plate 44, that is, vertically arranged, and both end portions of the elastic structure are respectively connected to the support plate 45 and the transmission seat 40. The transmission seat 40 penetrates through the mounting plate 44 for connecting the plunger pump 36. On the side where the plunger pump 36 is located, a base 41 connected to the transmission seat 40 can also be arranged, and the plunger of the plunger pump 36 is connected to the base 41.

[0066] The oil pumping unit further includes a guiding mechanism, and the guiding mechanism is arranged on the side of the mounting plate 44 where the transmission seat 40 is located. The guiding mechanism includes a linear pumping guide rail 47 arranged on one of the transmission seat 40 and the mounting plate 44, and a linear pumping guide groove 48 arranged on the other of the transmission seat 40 and the mounting plate 44. The pumping guide rail 47 is slidably connected to the pumping guide groove 48. There are two pumping guide rails 47 and two pumping guide grooves 48 respectively, which are correspondingly located on both sides of the lead screw. The linearity and stability of the driving operation are further ensured through the guiding mechanism. In this embodiment, the scheme of arranging the pumping guide rail 47 on the mounting plate 44 and arranging the pumping guide groove 48 on the transmission seat 40 is adopted.

[0067] A valve plate 49 is arranged on the mounting plate 44 on the side where the plunger pump 36 is located. A three-way valve corresponding to the plunger pump 36 is arranged on the valve plate 49. The pump body of the plunger pump 36 is conductively connected to the first port of the three-way valve on the valve plate 49. The second port of the three-way valve is communicated with the oil nozzle 20, and the third port of the three-way valve is communicated with the droplet generating gun head. Then, in different conducting states of the three-way valve, the oil can be transmitted from the oil supply unit to the plunger pump 36, or the oil in the plunger pump 36 can be pumped out and sent to the droplet generating gun head.

[0068] For the plunger pump 36, a liquid pipe is provided inside the pump body. The ratio of the inner diameter to the length of the liquid pipe is less than or equal to 1.2 / 30 and greater than 0. The preferred value of the ratio of the inner diameter to the length of the liquid pipe is 1 / 60. The plunger pump 36 with a slender liquid pipe structure can achieve a liquid flow control accuracy of nanoliters per second. Cooperating with the lead screw nut transmission mechanism that eliminates the vibration of the lead screw clearance, the plunger is pushed by the transmission seat 40, enabling the plunger to move at a uniform and stable speed, generating a stable flow pressure inside the plunger pump 36, and finally obtaining a stable flow output when pumping liquid.

[0069] As shown in the Figure 8 and Figure 9 accompanying drawings, the optical detection system 9 includes a light source 50, a camera 51, and a filter unit. The filter unit includes a transmitting-end filter wheel 52 arranged corresponding to the light source 50, a receiving-end filter wheel 52 arranged corresponding to the camera 51, a belt drive mechanism, and a switching drive motor 54. The belt drive mechanism is used to drive the transmitting-end filter wheel 52, the receiving-end filter wheel 52, and the switching drive motor 54 in-phase. When the switching drive motor 54 operates, the belt drive mechanism drives the transmitting-end filter wheel 52 and the receiving-end filter wheel 52 to rotate synchronously and in-phase to correspondingly switch the filter.

[0070] In this embodiment, the belt drive mechanism includes a synchronous belt 55, a main drive synchronous belt 55 wheel 56 coaxially connected to the output shaft of the drive motor, an active gear 71 coaxially connected to the main drive synchronous belt 55 wheel 56, a driven synchronous belt 55 wheel 57, and a first driven gear 58 coaxially connected to the driven synchronous belt 55 wheel 57. The synchronous belt 55 is wound between the main drive synchronous belt 55 wheel 56 and the driven synchronous belt 55 wheel 57. The active gear 71 is drivingly connected to the receiving-end filter wheel 52, and the first driven gear 58 is drivingly connected to the transmitting-end filter wheel 52. The belt drive mechanism further includes a plurality of tension wheels 60 distributed at appropriate positions and used to keep the synchronous belt 55 in a tensioned state to eliminate the phase deviation of the filter wheel.

[0071] Furthermore, the belt drive mechanism further includes a second driven gear 59. The second driven gear 59 is coaxially connected to the receiving-end filter wheel 52 and meshes with the active gear 71 for transmission. The circumferential surface of the transmitting-end filter wheel 52 is provided with teeth, and the first driven gear 58 meshes with the teeth of the transmitting-end filter wheel 52 for transmission.

[0072] In this embodiment, two driven synchronous belt 55 wheels 57 are symmetrically arranged on both sides of the main drive synchronous belt 55 wheel 56, and the two driven synchronous belt 55 wheels 57 are drivingly connected to the two transmitting-end filter wheels 52 in a one-to-one correspondence. A cooling fan may also be provided at the light source 50.

[0073] In the above solution, the three gear pairs formed between the driving gear 71 and the second driven gear 59, and between the corresponding two groups of first driven gears 58 and the transmitting end filter wheel 52 adopt the same reduction ratio. The number of teeth of the driving gear 71 and the two first driven gears 58 is the same, and the number of teeth is not limited. By driving each pulley and gear with a single switching drive motor 54, the three pulleys rotate in the same direction and have the same phase, so as to output a synchronous phase and achieve the purpose of synchronous rotation of the input end filter wheel and the output end filter wheel.

[0074] The box body 1 has a starting position, and a bottom plate 61 is arranged at the starting position. The bottom plate 61 has a first position corresponding to the droplet generation system 8, and a plurality of waste liquid grooves 62 are opened at the first position.

[0075] The thermal cycling system 10 arranged on the moving carrier platform 3 includes a placement groove for placing consumables and a heating unit for performing high and low temperature cycling heating on the consumables. The consumables include an amplification container 64 and a cover plate 65. The bottom plate 61 is used for temporarily placing the cover plate 65 during the droplet generation process. Then, the digital PCR device further includes a first transfer mechanism arranged at the bottom of the moving detection platform 2 for moving the cover plate 65 of the consumables from the placement groove to the first position of the bottom plate 61. A plurality of magnetic metal sheets 66 can be arranged on the cover plate 65, and it is set that the first transfer mechanism includes a plurality of magnetic adsorbing components embedded at the bottom of the moving detection platform 2 and corresponding one-to-one to the magnetic metal sheets 66 on the cover plate 65. The magnetic adsorbing components are preferably electromagnets. Then, when the electromagnet is energized, the cover plate 65 can be adsorbed, and the cover plate 65 is driven by the movement of the moving carrier platform 3 to achieve the transfer of its position.

[0076] Since the mobile detection platform 2 only has the function of moving in the X-axis direction, when the cover plate 65 is transferred to the bottom plate 61, the bottom plate 61 will cover the waste liquid tank 62. Therefore, the position of the cover plate 65 needs to be transferred again, that is, the bottom plate 61 also has a second position on the side of the first position, and the digital PCR device further includes a second transfer mechanism for transferring the cover plate 65 of the consumable from the first position to the second position. The second transfer mechanism includes a carrier plate 67 slidably arranged on the bottom plate 61 for carrying the cover plate 65, a push plate 68 arranged at the edge of the carrier plate 67, and a translation driving member 6926 arranged on the side of the mobile carrier platform 3 and capable of contacting the push plate 68. When the translation driving member 6926 translates along the Y-axis direction with the mobile carrier platform 3, the translation driving member 6926 drives the carrier plate 67 to move along the Y-axis direction through the push plate 68. A preferred way is to provide a hanging hole on the push plate 68, and the translation driving member 6926 has a hanging rod that can extend or retract. When the hanging rod extends, it is inserted into the hanging hole. The translation driving member 6926 can be an electric driving device. Further, the second transfer mechanism further includes a translation guide rail 70 arranged on one of the bottom plate 61 and the carrier plate 67, and a translation guide groove arranged on the other of the bottom plate 61 and the carrier plate 67. The translation guide rail 70 is slidably connected to the translation guide groove. The translation guide rail 70 is linear to guide the movement of the carrier plate 67 carrying the cover plate 65.

[0077] The droplet generation system 8 outputs droplets into the consumable. After amplification is completed under the action of the thermal cycling system 10, it is sent to the optical detection system 9 for detection to obtain the detection result.

[0078] The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and shall not be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit and essence of the present invention shall be covered within the protection scope of the present invention.

Claims

1. A digital PCR device, characterized in that: include: A droplet generation system, the droplet generation system comprising an oil supply unit for providing oil, an oil pumping unit for obtaining oil from the oil supply unit and pumping the oil, a droplet generation gun head for obtaining the oil pumped by the oil pumping unit and capable of sucking samples and outputting droplets, and a droplet generation unit; The oil supply unit comprises an oil container having an oil accommodating space and a liquid outlet, an oil nozzle capable of being driven to move along a preset path and having a first position and a second position, and a locking device for keeping the oil nozzle in the first position, the oil nozzle having an internal passage, and the oil supply unit having a first state and a second state. When in the first state, the oil nozzle is in the first position, the oil nozzle is tightly connected to the oil container, and the internal passage of the oil nozzle is communicated with the liquid outlet of the oil container; when in the second state, the oil nozzle is in the second position, the oil nozzle is separated from the oil container, and the internal passage of the oil nozzle is disconnected from the liquid outlet of the oil container; An optical detection system, the optical detection system includes a light source, a camera and a filter unit, the filter unit includes a transmitting end filter wheel corresponding to the light source, a receiving end filter wheel corresponding to the camera, a pulley transmission mechanism and a switching drive motor, the pulley transmission mechanism is used to connect the transmitting end filter wheel, the receiving end filter wheel and the switching drive motor through in-phase transmission, when the switching drive motor is working, the pulley transmission mechanism drives the transmitting end filter wheel and the receiving end filter wheel to rotate synchronously in the same phase to correspond to the switching filter.

2. The digital PCR device according to claim 1, characterized in that: The pulley transmission mechanism includes a synchronous belt, a main drive synchronous pulley coaxially connected to the output shaft of the drive motor, a driving gear coaxially connected to the main drive synchronous pulley, a driven synchronous pulley, and a first driven gear coaxially connected to the driven synchronous pulley, the synchronous belt is wound between the main drive synchronous pulley and the driven synchronous pulley, the driving gear is drivingly connected to the receiving end filter wheel, and the first driven gear is drivingly connected to the transmitting end filter wheel.

3. The digital PCR device according to claim 2, characterized in that: The pulley transmission mechanism also includes a second driven gear, which is coaxially connected to the receiving end filter wheel and meshes with the driving gear for transmission. The circumference of the transmitting end filter wheel is provided with gear teeth, and the first driven gear meshes with the gear teeth of the transmitting end filter wheel for transmission.

4. The digital PCR device according to claim 2, characterized in that: The pulley transmission mechanism also includes a plurality of tensioning wheels for keeping the synchronous belt in a tensioned state.

5. The digital PCR device according to claim 2, characterized in that: Two driven synchronous belt wheels are symmetrically arranged on both sides of the main driving synchronous belt wheel, and the two driven synchronous belt wheels are transmission-connected with the two emission-end filter wheels in a one-to-one correspondence.

6. The digital PCR device according to claim 1, characterized in that: The oil supply unit also includes a driving device for driving the oil nozzle to move along the preset path between the first position and the second position; the driving device includes a rotatable driving member and a trigger ring formed on the outer periphery of the liquid nozzle. When the driving member rotates, it drives the liquid nozzle to move in a straight line by abutting against the trigger ring.

7. The digital PCR device according to claim 6, characterized in that: The oil supply unit includes a support, the support has a first mounting portion, the oil nozzle is arranged on the first mounting portion, the driving member has a driving arm and a connecting portion rotatably connected to the first mounting portion, and the trigger ring includes an upper trigger ring and a lower trigger ring which are arranged at intervals and respectively located on both sides of the driving arm.

8. The digital PCR device according to claim 7, characterized in that: The driving device also includes an operating handle connected to the driving member, and the locking device includes a clamping groove arranged on the oil container and a clamping joint formed at one end of the operating handle. When in the first state, the clamping joint is embedded in the clamping groove.

9. The digital PCR device according to claim 1, characterized in that: The liquid outlet portion includes a liquid outlet hole and a sealing ring arranged on the hole wall of the liquid outlet hole; the oil nozzle includes a liquid pipe with the internal channel, the liquid pipe is opposite to the sealing ring, and one end of the liquid pipe facing the sealing ring has a slope; the end face of the sealing ring facing the liquid pipe also has a slope; when in the first state, the slope of the liquid pipe is pressed against the slope of the sealing ring.

10. The digital PCR device according to claim 9, characterized in that: The oil container is provided with a mounting hole, an oil container cover is fixedly arranged in the mounting hole, the liquid outlet is arranged on the oil container cover, and an air vent is also arranged on the oil container cover; and / or, an edge of the mounting hole is provided with a hole wall located outside the oil container, and the outer periphery of the oil container cover is provided with a convex edge which abuts against the end surface of the hole wall.

11. The digital PCR device according to claim 1, characterized in that: The oil supply unit further comprises a guide seat for guiding the movement of the liquid nozzle along a preset path, and the guide seat is arranged around the oil nozzle.

12. The digital PCR device according to claim 1, characterized in that: The oil pumping unit includes a plunger pump, a pumping motor, a screw nut transmission mechanism connecting the pumping motor and the plunger pump, and an elastic structure. The plunger pump includes a pump body and a plunger slidably connected to the pump body. The screw nut transmission mechanism includes a pumping screw, a pumping nut and a transmission seat. The pumping screw is connected to the pumping motor, the pumping nut is connected to the transmission seat, and the transmission seat is connected to the plunger of the plunger pump. The screw nut transmission mechanism drives the plunger to move in a first direction under the drive of the pumping motor to achieve liquid pumping. The elastic structure is used to provide a reverse thrust to the plunger in the opposite direction to the first direction when the plunger moves in the first direction. The pump body of the plunger pump is connected to the first port of the three-way valve, the second port of the three-way valve is connected to the oil nozzle, and the third port of the three-way valve is connected to the droplet generation gun head.

13. The digital PCR device according to claim 12, characterized in that: The elastic structure includes an elastic member, and the axial extension direction of the elastic member is consistent with the first direction; the elastic member is sleeved on the outer periphery of the pumping screw, or the elastic member includes a first elastic member and a second elastic member symmetrically distributed on both sides of the pumping screw; when the plunger moves toward the first direction, the elastic force generated by the compression of the elastic member forms the reverse thrust, and the elastic member is configured so that the reverse thrust formed by its compression is always smaller than the driving force of the screw nut transmission mechanism on the plunger.

14. The digital PCR device according to claim 13, characterized in that: The elastic member is a spring.

15. The digital PCR device according to claim 12, characterized in that: The oil pumping unit includes a vertically extending mounting plate and a support plate horizontally arranged on the mounting plate, a pair of connecting seats are arranged on the mounting plate, the pumping screw is arranged vertically and its two ends are rotatably connected to the pair of connecting seats respectively, the nut is threadedly connected to the pumping screw, the transmission seat is connected to the mounting plate, the elastic structure is arranged vertically, and the two ends of the elastic structure are respectively connected to the support plate and the transmission seat; the transmission seat is arranged through the mounting plate, the elastic structure, the pumping screw and the pumping nut are located on one side of the mounting plate, and the plunger pump is located on the other side of the mounting plate opposite to the one side.

16. The digital PCR device according to claim 12, characterized in that: The oil pumping unit also includes a guiding mechanism, which includes a pumping guide rail arranged on one of the transmission seat and the mounting plate, and a pumping guide groove arranged on the other of the transmission seat and the mounting plate, and the pumping guide rail is slidably connected to the pumping guide groove.

17. The digital PCR device according to claim 1, characterized in that: The digital PCR device includes a housing, a mobile detection platform movably arranged on the housing, a mobile supporting platform movably arranged in the housing, an X-axis moving mechanism, an X-axis driving mechanism, a Y-axis moving mechanism and a Y-axis driving mechanism; the droplet generation system and the optical detection system are both arranged on the mobile detection platform, the X-axis moving mechanism is connected between the housing and the X-axis driving mechanism, and when the X-axis driving mechanism is working, the mobile detection platform is driven to translate along the X-axis direction through the X-axis moving mechanism, and the Y-axis moving mechanism is connected between the housing and the Y-axis driving mechanism, and when the Y-axis driving mechanism is working, the mobile supporting platform is driven to translate along the Y-axis direction through the Y-axis moving mechanism.

18. The digital PCR device according to claim 17, characterized in that: The X-axis moving mechanism includes an X-axis synchronous belt that transmits along the X-axis direction, a plurality of synchronous wheels, connecting pieces respectively connected to the mobile detection platform and the X-axis synchronous belt, an X-axis guide rail parallel to the X-axis direction arranged on one of the box and the mobile detection platform, and an X-axis guide groove arranged on the other of the box and the mobile detection platform; the X-axis synchronous belt is wound around each of the synchronous wheels, at least one of the synchronous wheels is transmission-connected to the X-axis driving mechanism; the X-axis guide rail is slidably connected to the X-axis guide groove; a plurality of X-axis sliders are arranged at the bottom of the mobile detection platform, and the X-axis guide rail or the X-axis guide groove is arranged on the X-axis slider; and / or, The Y-axial moving mechanism includes a Y-axial driving screw axially arranged along the Y-axial direction, a Y-axial moving nut threadedly connected to the Y-axial driving screw, a Y-axial guide rail parallel to the Y-axial direction arranged on one of the box body and the movable supporting platform, and a Y-axial guide groove arranged on the other of the box body and the movable supporting platform, the movable supporting platform is connected to the Y-axial moving nut, and the Y-axial guide rail is slidably connected to the Y-axial guide groove; a plurality of Y-axial sliding blocks are arranged at the bottom of the movable supporting platform, and the Y-axial guide rail or the Y-axial guide groove is arranged on the Y-axial sliding block.

19. The digital PCR device according to claim 17, characterized in that: The digital PCR device further comprises a plurality of thermal cycle systems disposed on the mobile carrying platform, wherein the thermal cycle systems comprise a placement slot for placing consumables and a heating unit for performing high and low temperature cycle heating on the consumables; The box has a starting position, a bottom plate is arranged at the starting position, a first position corresponding to the droplet generation system is arranged on the bottom plate, and a plurality of waste liquid tanks are arranged at the first position; the consumables include an amplification container and a cover plate, and the digital PCR device also includes a first transfer mechanism for moving the cover plate of the consumables from the placement slot to the first position of the bottom plate, and the first transfer mechanism is arranged at the bottom of the mobile detection platform; A plurality of magnetic metal sheets are arranged on the cover plate, and the first transfer mechanism comprises a plurality of magnetic adsorption members which are embedded in the bottom of the mobile detection platform and correspond one-to-one to the magnetic metal sheets on the cover plate.

20. The digital PCR device according to claim 19, characterized in that: The magnetic adsorption component is an electromagnet.

21. The digital PCR device according to claim 19, characterized in that: The bottom plate also has a second position located at the side of the first position, and the digital PCR device also includes a second transfer mechanism for transferring the cover plate of the consumable from the first position to the second position; The second transfer mechanism includes a carrying plate slidably arranged on the base plate for carrying the cover plate, a push plate arranged on the edge of the carrying plate, a translation driving member arranged on the side of the mobile carrying platform and capable of contacting the push plate, a translation guide rail arranged on one of the base plate and the carrying plate, and a translation guide groove arranged on the other of the base plate and the carrying plate, wherein the translation guide rail is slidably connected to the translation guide groove; when the translation driving member translates along the Y-axis direction with the mobile carrying platform, the translation driving member drives the carrying plate to move along the Y-axis direction through the push plate.

22. The digital PCR device according to claim 21, characterized in that: The push plate is provided with a hooking hole, and the translation driving member has a hooking rod that can be extended or retracted, and when the hooking rod is extended, it is inserted into the hooking hole.

Images