Fermentation tank sampling device for mycoplasma vaccine preparation

Abstract

The invention relates to the technical field of sampling devices, in particular to a fermentation tank sampling device for mycoplasma vaccine preparation. The sampling valve is fixedly arranged on the side wall of the fermentation tank; the discharging assembly comprises a discharging pipe, the first end of the discharging pipe is connected with the sampling valve, a liquid inlet channel and an air inlet channel are formed in the discharging pipe, the first end of the liquid inlet channel and the first end of the air inlet channel both penetrate through the inner surface of the discharging pipe, and the second end of the liquid inlet channel and the second end of the air inlet channel both penetrate through the outer surface of the discharging pipe. When the device is used, water flow can clean the sampling valve through the liquid inlet channel in the discharging pipe, residues of contents in the fermentation tank are avoided at the outlet position of the sampling valve, and high-temperature and high-pressure sterilization operation can be completed by high-pressure steam through the air inlet channel in the discharging pipe.

Description

Technical Field

[0001] The present invention relates to the technical field of sampling devices, and specifically to a sampling device for a fermenter used in the preparation of mycoplasma vaccines. Background Art

[0002] Mycoplasma vaccines usually refer to Mycoplasma pneumoniae vaccines. The fermenter provides a controllable environment for the growth and metabolism of microorganisms. During the preparation of mycoplasma vaccines, it is necessary to screen out mycoplasma strains with weakened virulence but good retained immunogenicity. After sampling the substances in the fermenter, sterilization operations need to be carried out to prevent bacteria from growing at the valves. For example, the Chinese utility model patent with the patent application number "CN202321538401.3" provides a sampling device for a fermenter used in the preparation of viral vaccines. This device forms an overall passage through a sterilization ventilation mechanism, a tank body sampling valve, a fermentation broth sampling mechanism, and a sterilization ventilation mechanism. High-pressure steam sequentially passes through a high-pressure steam inlet pipe, a sampling exhaust valve, a tank body sampling valve, a fermentation broth sampling mechanism, and a high-pressure steam outlet pipe to perform high-temperature and high-pressure sterilization on the device.

[0003] The disadvantage of this device is that after sampling, high-pressure steam will directly enter the high-pressure steam inlet pipe. That is to say, before the sterilization operation, the tank body sampling valve cannot be cleaned, resulting in residues of the fermenter contents at the outlet position of the tank body sampling valve. Summary of the Invention

[0004] The purpose of the present invention is to provide a sampling device for a fermenter used in the preparation of mycoplasma vaccines. The sampling valve is connected to a discharge assembly. The discharge assembly includes a discharge pipe. Water flow can clean the sampling valve through the liquid inlet channel inside the discharge pipe, and there will be no residue of the fermenter contents at the outlet position of the sampling valve. High-pressure steam can complete high-temperature and high-pressure sterilization operations through the air inlet channel inside the discharge pipe.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A sampling device for a fermenter used in the preparation of mycoplasma vaccines, comprising: a fermenter; a sampling valve fixedly arranged on the side wall of the fermenter; a discharge assembly, the discharge assembly includes a discharge pipe, a first end of the discharge pipe is connected to the sampling valve, and the inside of the discharge pipe is provided with a liquid inlet channel and an air inlet channel. The first ends of the liquid inlet channel and the air inlet channel both penetrate the inner surface of the discharge pipe, and the second ends of the liquid inlet channel and the air inlet channel both penetrate the outer surface of the discharge pipe.

[0006] Preferably, there are three liquid inlet channels and three gas inlet channels respectively, and the liquid inlet channels and the gas inlet channels are arranged at intervals; an assembly tank is sleeved on the outer surface of the fermentation tank, a partition plate is arranged inside the assembly tank, and the partition plate divides the inside of the fermentation tank into a first assembly cavity and a second assembly cavity. The first assembly cavity is located above the second assembly cavity, and the sampling valve and the discharge pipe are both located in the first assembly cavity; the discharge assembly further includes: an adjusting ring, arranged inside the discharge pipe and located at one end close to the sampling valve, the adjusting ring is rotatably connected to the discharge pipe, and three through holes are arranged on the adjusting ring at equal intervals; an adjusting cylinder, arranged inside the discharge pipe and located at one end far from the sampling valve, the adjusting cylinder can slide along the inner wall of the discharge pipe, and three adjusting blocks are fixedly arranged at one end of the adjusting cylinder close to the adjusting ring at equal intervals. Three adjusting grooves are arranged on the inner side wall of the adjusting ring, and the positions of the three adjusting blocks and the three adjusting grooves correspond one by one. Each adjusting groove is provided with one of the through holes; a discharge guiding cover is fixedly arranged on the outer side wall of the assembly tank and corresponds to the position of the discharge pipe. A first opening is arranged on the side wall of the discharge guiding cover close to the discharge pipe, and a second opening is arranged at the bottom of the discharge guiding cover. Both the first opening and the second opening communicate with the inside of the discharge guiding cover.

[0007] Preferably, the cross-sectional shape of the adjusting block is triangular, the shape of the adjusting groove matches the shape of the adjusting block, and the adjusting block can slide along the inner wall of the adjusting groove; on the inner side wall of the discharge pipe near the adjusting ring end, a number of metal plates are fixedly arranged at equal intervals, and on the outer side wall of the adjusting ring, a number of magnets are fixedly arranged at equal intervals; at the end of the adjusting cylinder away from the adjusting ring, there are three transfer ports arranged at equal intervals, one of the transfer ports is located at the top of the adjusting cylinder, and its opening area is larger than the other two transfer ports; the discharge assembly further includes a first driving assembly, an annular assembly groove is arranged on the inner side wall of the discharge pipe, which is located at the end away from the adjusting ring, and the first driving assembly is arranged inside the annular assembly groove. The first driving assembly includes: an annular rack, sleeved on the outer surface of the adjusting cylinder and fixedly connected with the adjusting cylinder; an electric telescopic rod, horizontally arranged above the adjusting cylinder and fixedly connected with the discharge pipe; an arc-shaped frame, arranged between the annular rack and the electric telescopic rod, the arc-shaped frame is sleeved on the outer surface of the adjusting cylinder and can slide along the outer surface of the adjusting cylinder, the arc-shaped frame is fixedly connected with the output end of the electric telescopic rod, a limiting block is arranged between the annular rack and the arc-shaped frame, a guiding groove is arranged on the side wall of the arc-shaped frame, the first end of the limiting block is fixedly connected with the annular rack, the second end of the limiting block extends into the guiding groove and can slide along the guiding groove, and the width of the second end of the limiting block is greater than the width of the first end of the limiting block; a first motor, fixedly arranged on the arc-shaped frame, the output end of the first motor is fixedly connected with a first gear, and the first gear is in meshing transmission with the annular rack.

[0008] Preferably, it further includes a flushing and disinfection assembly, and the flushing and disinfection assembly includes: a liquid inlet assembly, arranged in the first assembly cavity, for conveying flushing liquid to the liquid inlet channel; an air inlet assembly, arranged in the first assembly cavity, for conveying high-pressure steam to the air inlet channel.

[0009] Preferably, the liquid inlet assembly includes: a first annular pipe, a first branch pipe is arranged at the top of the first annular pipe, the first end of the first branch pipe is communicated with the inside of the first annular pipe, and the second end of the first branch pipe extends to the outside of the assembly tank; a second annular pipe, sleeved on the outside of the discharge pipe, a second branch pipe is arranged between the second annular pipe and the first annular pipe, both ends of the second branch pipe are respectively communicated with the inside of the first annular pipe and the second annular pipe, a first electric control valve is arranged in the middle of the second branch pipe, and three third branch pipes are arranged between the second annular pipe and the discharge pipe, the first ends of the three third branch pipes are all communicated with the inside of the second annular pipe, and the second ends of the three third branch pipes are respectively communicated with the second ends of the three liquid inlet channels.

[0010] Preferably, the intake assembly includes: a third annular pipe, a fourth branch pipe is provided at the top of the third annular pipe, a first end of the fourth branch pipe communicates with the inside of the third annular pipe, and a second end of the fourth branch pipe extends to the outside of the assembly tank; a fourth annular pipe, sleeved outside the discharge pipe, a fifth branch pipe is provided between the fourth annular pipe and the third annular pipe, both ends of the fifth branch pipe communicate with the inside of the third annular pipe and the fourth annular pipe respectively, a second electric control valve is provided in the middle of the fifth branch pipe, and three sixth branch pipes are provided between the fourth annular pipe and the discharge pipe, first ends of the three sixth branch pipes all communicate with the inside of the fourth annular pipe, and second ends of the three sixth branch pipes communicate with second ends of the three intake channels respectively.

[0011] Preferably, the flushing and disinfection assembly further includes a waste recycling assembly, and the waste recycling assembly includes: a recycling box, which is arranged in the second assembly cavity and can slide along the inner wall of the second assembly cavity, and the top surface of the recycling box is open; a cleaning pipe, arranged outside the assembly tank, the cleaning pipe has an L-shaped structure, the second opening penetrates through both side walls of the discharge guiding cover, an avoidance opening is provided at the bottom of the outer side wall of the assembly tank, a first end of the cleaning pipe is arranged in the second opening and can slide along the inner wall of the second opening, a second end of the cleaning pipe passes through the avoidance opening and is fixedly connected to the middle of the side wall of the recycling box, and the second end of the cleaning pipe communicates with the inside of the recycling box; a second driving assembly, arranged outside the assembly tank, the second driving assembly includes an arc-shaped rack, a first end of the arc-shaped rack is fixedly connected to the cleaning pipe, a second motor is fixedly arranged on the outer side wall of the assembly tank, an output end of the second motor is fixedly connected to a second gear, and the second gear meshes with the arc-shaped rack for transmission.

[0012] Preferably, it further includes a sampling auxiliary assembly, the sampling auxiliary assembly is arranged outside the assembly tank, and the sampling auxiliary assembly includes: a first isolation cover, fixedly arranged on the outer side wall of the assembly tank and located on the side of the cleaning pipe away from the second motor; a second isolation cover, arranged directly below the first isolation cover and fixedly connected to the outer side wall of the assembly tank, receiving openings are provided on the opposite side walls of the first isolation cover and the second isolation cover; a transfer pipe, vertically arranged between the first isolation cover and the second isolation cover, both ends of the transfer pipe are arranged in the two receiving openings respectively; an arc-shaped rod, a first end of the arc-shaped rod is detachably connected to the transfer pipe, a second end of the arc-shaped rod is fixedly connected to the cleaning pipe, and an annular slide rail is sleeved on the outer side wall of the assembly tank, and the arc-shaped rod can slide along the annular slide rail.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] (1) The present invention includes a fermentation tank, a sampling valve, and a discharging assembly. The discharging assembly includes a discharging pipe, and an inlet liquid passage and an inlet gas passage are provided inside the discharging pipe. After the sampling valve is opened, the content of the fermentation tank will enter the discharging pipe through the sampling valve and finally flow out of the discharging pipe to complete sampling. After sampling, water flow can clean the sampling valve through the inlet liquid passage inside the discharging pipe, and there will be no residue of the content of the fermentation tank at the outlet position of the sampling valve. High-pressure steam can complete the high-temperature and high-pressure sterilization operation through the inlet gas passage inside the discharging pipe.

[0015] (2) The present invention further includes an assembly tank, a flushing and disinfection assembly, and a sampling auxiliary assembly. The flushing and disinfection assembly can convey flushing liquid to the inlet liquid passage, convey high-pressure steam to the inlet gas passage, and recover the water flow after cleaning. The sampling auxiliary assembly can drive the flushing and disinfection assembly to move, facilitating the completion of sampling, cleaning, and high-temperature sterilization tasks. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is an axonometric view of the present invention;

[0017] Figure 2 is a top view cross-sectional view of the present invention;

[0018] Figure 3 is an axonometric view of the fermentation tank in the present invention;

[0019] Figure 4 is an axonometric view of the assembly tank in the present invention;

[0020] Figure 5 is an axonometric view of the discharging assembly in the present invention;

[0021] Figure 6 is an axonometric exploded view of the discharging assembly in the present invention;

[0022] Figure 7 is an axonometric cross-sectional view of the discharging pipe in the present invention;

[0023] Figure 8 is an axonometric view of the adjusting ring in the present invention;

[0024] Figure 9 is an axonometric view of the adjusting cylinder at one angle in the present invention;

[0025] Figure 10 is an axonometric view of the adjusting cylinder at another angle in the present invention;

[0026] Figure 11 is an axonometric exploded view of the first driving assembly in the present invention;

[0027] Figure 12 is a front view cross-sectional view of the discharging assembly in the present invention when the discharging pipe is not moved;

[0028] Figure 13 An isometric view of the discharge assembly in the present invention after the discharge pipe moves;

[0029] Figure 14 A front view sectional view of the discharge assembly in the present invention after the discharge pipe moves;

[0030] Figure 15 An isometric view of the discharge assembly in the present invention after the discharge pipe rotates;

[0031] Figure 16 A front view sectional view of the discharge assembly in the present invention after the discharge pipe rotates;

[0032] Figure 17 An isometric view of the flushing and disinfection assembly in the present invention;

[0033] Figure 18 An isometric view of the liquid inlet assembly in the present invention;

[0034] Figure 19 An isometric view of the air inlet assembly in the present invention;

[0035] Figure 20 An isometric view of the waste recycling assembly in the present invention;

[0036] Figure 21 An isometric exploded view of the sampling auxiliary assembly in the present invention;

[0037] Figure 22 An isometric view of the present invention after the transfer pipe moves to the lower part of the discharge guiding cover.

[0038] Reference numerals include:

[0039] 1 - Fermentation tank, 2 - Sampling valve, 3 - Discharge assembly, 31 - Discharge pipe, 311 - Liquid inlet channel, 312 - Air inlet channel, 313 - Annular assembly groove, 32 - Adjusting ring, 321 - Through hole, 322 - Adjusting groove, 33 - Adjusting cylinder, 331 - Adjusting block, 332 - Transfer port, 34 - Discharge guiding cover, 341 - First opening, 342 - Second opening, 35 - Metal plate, 36 - Magnet, 37 - First driving assembly, 371 - Annular rack, 372 - Electric telescopic rod, 373 - Arc-shaped frame, 3731 - Guide groove, 374 - Limit block, 375 - First motor, 376 - First gear, 4 - Assembly tank, 41 - Partition board, 42 - First assembly cavity, 43 - Second assembly cavity, 44 - Avoidance opening, 45 - Annular slide rail, 5 - Flushing and disinfection assembly, 51 - Liquid inlet assembly, 511 - First annular pipe, 512 - First branch pipe, 513 - Second annular pipe, 514 - Second branch pipe, 515 - First electric control valve, 516 - Third branch pipe, 52 - Air inlet assembly, 521 - Third annular pipe, 522 - Fourth branch pipe, 523 - Fourth annular pipe, 524 - Fifth branch pipe, 525 - Second electric control valve, 526 - Sixth branch pipe, 53 - Waste recycling assembly, 531 - Recycling box, 532 - Cleaning pipe, 533 - Second driving assembly, 5331 - Arc-shaped rack, 5332 - Second motor, 5333 - Second gear, 6 - Sampling auxiliary assembly, 61 - First isolation cover, 62 - Second isolation cover, 63 - Storage opening, 64 - Material transfer pipe, 65 - Arc-shaped rod. Detailed implementation manners

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0041] Embodiment 1

[0042] Please refer to Figures 1-22, the present invention provides a technical solution: a sampling device for preparing mycoplasma vaccine using a fermenter, which includes a fermenter 1, a sampling valve 2 and a discharging component 3. The discharging component 3 includes a discharging pipe 31, and an inlet liquid channel 311 and an inlet gas channel 312 are arranged inside the discharging pipe 31. When the sampling valve 2 is opened, the content of the fermenter 1 will enter the discharging pipe 31 through the sampling valve 2 and finally flow out of the discharging pipe 31 to complete sampling. When the sampling is completed, first, water flow will enter the discharging pipe 31 through the inlet liquid channel 311, and then the outlet position of the sampling valve 2 and the inside of the discharging pipe 31 will be cleaned. Then, high-pressure steam will enter the discharging pipe 31 through the inlet gas channel 312, and then high-temperature and high-pressure sterilization operation will be carried out on the outlet position of the sampling valve 2 and the inside of the discharging pipe 31. In this embodiment, there are three inlet liquid channels 311 and three inlet gas channels 312 respectively, and the inlet liquid channels 311 and the inlet gas channels 312 are arranged at intervals. Therefore, it can be ensured that whether it is water flow or high-pressure steam, after entering the discharging pipe 31, it can fully clean and sterilize the outlet position of the sampling valve 2 and the inside of the discharging pipe 31, and there will be no residue of the fermenter content at the outlet position of the sampling valve 2..

[0043] Please refer to Figures 1-16 , the discharging component 3 further includes an adjusting ring 32, an adjusting cylinder 33, a discharging guiding cover 34 and a first driving component 37. Three through holes 321 are arranged on the adjusting ring 32 at equal intervals. Three adjusting blocks 331 are fixedly arranged at one end of the adjusting cylinder 33 close to the adjusting ring 32. Three adjusting grooves 322 are arranged on the inner side wall of the adjusting ring 32, and each adjusting groove 322 is provided with a through hole 321. A first opening 341 is arranged on the side wall of the discharging guiding cover 34 close to the discharging pipe 31, and a second opening 342 is arranged at the bottom of the discharging guiding cover 34. An annular assembly groove 313 is arranged on the inner side wall of the discharging pipe 31, and the first driving component 37 is arranged inside the annular assembly groove 313, and the output end of the first driving component 37 is connected to the adjusting cylinder 33.

[0044] Please refer to Figures 1-16 , when the sampling valve 2 is opened for sampling, the right end of the adjusting cylinder 33 is closely attached to the adjusting ring 32, and the left end of the adjusting cylinder 33 does not enter the discharging guiding cover 34. The adjusting blocks 331 are inserted into the adjusting grooves 322, and the three adjusting blocks 331 block the through holes 321 communicated with the three inlet liquid channels 311, and the adjusting ring 32 itself blocks the three inlet gas channels 312. At this time, the content of the fermenter 1 will enter the discharging pipe 31 through the sampling valve 2, then enter the discharging guiding cover 34 through the first opening 341, and finally be discharged through the second opening 342 to complete the sampling operation.

[0045] Please refer to Figures 1-16, after the sampling is completed, first, the first driving component 37 operates to drive the left end of the adjusting cylinder 33 into the discharge guiding cover 34, and the adjusting block 331 leaves the adjusting groove 322. The three adjusting blocks 331 no longer block the through holes 321 communicating with the three liquid inlet channels 311. Water flows through the three liquid inlet channels 311 and the three through holes 321 into the discharge pipe 31, and then the outlet position of the sampling valve 2, the discharge pipe 31, the adjusting ring 32, the adjusting cylinder 33, and the discharge guiding cover 34 are cleaned, and the water flows out from the second opening 342.

[0046] Please refer to Figures 1-16 , after the cleaning process is completed, first, the first driving component 37 operates to drive the right end of the adjusting cylinder 33 to closely adhere to the adjusting ring 32 again, and the adjusting block 331 is inserted into the adjusting groove 322 again. Subsequently, first, the first driving component 37 continues to operate to drive the adjusting cylinder 33 to rotate, so that the three through holes 321 communicate with the three air inlet channels 312, and the adjusting ring 32 itself blocks the three liquid inlet channels 311. Then the first driving component 37 continues to operate to drive the left end of the adjusting cylinder 33 into the discharge guiding cover 34, and the adjusting block 331 leaves the adjusting groove 322. The three adjusting blocks 331 no longer block the through holes 321 communicating with the three air inlet channels 312. High-pressure steam will enter the discharge pipe 31 through the air inlet channels 312, and then the high-temperature and high-pressure sterilization operation of the outlet position of the sampling valve 2, the discharge pipe 31, the adjusting ring 32, the adjusting cylinder 33, and the discharge guiding cover 34 is completed, and the high-pressure steam is discharged from the second opening 342.

[0047] Please refer to Figures 1-16 , the cross-sectional shape of the adjusting block 331 is triangular, and the shape of the adjusting groove 322 matches the shape of the adjusting block 331. The adjusting block 331 can slide along the inner wall of the adjusting groove 322. Therefore, the adjusting block 331 can fit with the inner wall of the adjusting groove 322, and there will be no liquid leakage or air leakage, and the water flow or high-pressure steam can directly flow along the inner wall of the adjusting groove 322 to the outlet position of the sampling valve 2.

[0048] Please refer to Figures 1-16 , a plurality of equally spaced metal plates 35 are fixedly arranged on the inner side wall of the discharge pipe 31 near one end of the adjusting ring 32, and a plurality of equally spaced magnets 36 are fixedly arranged on the outer side wall of the adjusting ring 32. When the first driving component 37 drives the adjusting cylinder 33 to rotate, the magnet 36 can adsorb the metal plate 35. Therefore, even if the adjusting block 331 leaves the adjusting groove 322, the adjusting ring 32 will not rotate in the reverse direction, ensuring that the through hole 321 can always correctly communicate with the liquid inlet channel 311 or the air inlet channel 312.

[0049] Please refer to Figures 1-16, at one end of the adjusting cylinder 33 away from the adjusting ring 32, there are three transmission ports 332 evenly distributed at equal intervals. When the adjusting block 331 leaves the adjusting groove 322, the left end of the adjusting cylinder 33 can be attached to the inner wall of the discharge guiding cover 34. At this time, water flow will flow out of the adjusting cylinder 33 through the three transmission ports 332. In addition, in this embodiment, one of the transmission ports 332 is located at the top of the adjusting cylinder 33, and its opening area is larger than the other two transmission ports 332. Therefore, the flow rate of the water flow discharged through the top transmission port 332 is greater than that of the water flow discharged through the other two transmission ports 332. Thus, the water flow can fully clean the interior of the discharge guiding cover 34. After the first driving assembly 37 drives the adjusting cylinder 33 to rotate, high-pressure steam will also enter the discharge guiding cover 34 through the three transmission ports 332 to complete a full high-temperature and high-pressure sterilization operation on the interior of the discharge guiding cover 34.

[0050] Please refer to Figures 1-16 , the first driving assembly 37 includes an annular rack 371, an electric telescopic rod 372, an arc-shaped frame 373, a limiting block 374, a first motor 375 and a first gear 376. A guiding groove 3731 is provided on the side wall of the arc-shaped frame 373, and the limiting block 374 extends into the guiding groove 3731. When the first driving assembly 37 works, the electric telescopic rod 372 extends, driving the arc-shaped frame 373 and the annular rack 371 to move to the left together, and then driving the adjusting cylinder 33 to move to the left. The first motor 375 drives the first gear 376 to rotate, which can drive the annular rack 371 to rotate, and then drive the adjusting cylinder 33 to rotate. At this time, the arc-shaped frame 373 slides along the outer surface of the adjusting cylinder 33, and the limiting block 374 slides along the guiding groove 3731. The electric telescopic rod 372 shortens, driving the arc-shaped frame 373 to move to the right, and then driving the annular rack 371 to move to the right together through the limiting block 374, and finally driving the adjusting cylinder 33 to move to the right.

[0051] Embodiment 2

[0052] Please refer to Figures 1-17 , the present invention further includes an assembly tank 4 and a flushing and disinfection assembly 5. The assembly tank 4 is sleeved on the outer surface of the fermentation tank 1. A partition plate 41 is provided inside the assembly tank 4, and the partition plate 41 divides the interior of the fermentation tank 1 into a first assembly cavity 42 and a second assembly cavity 43. The flushing and disinfection assembly 5 includes a liquid inlet assembly 51, a gas inlet assembly 52 and a waste recycling assembly 53. The liquid inlet assembly 51 is arranged in the first assembly cavity 42 and is used to convey flushing liquid to the liquid inlet channel 311. The gas inlet assembly 52 is arranged in the first assembly cavity 42 and is used to convey high-pressure steam to the gas inlet channel 312. The waste recycling assembly 53 is arranged below the discharge guiding cover 34 and is used to recycle the water flow after cleaning.

[0053] Please refer to Figures 1-18, the liquid inlet assembly 51 includes a first annular pipe 511, a first branch pipe 512, a second annular pipe 513, a second branch pipe 514, a first electric control valve 515 and three third branch pipes 516. In the present invention, there are three sampling valves 2 and three discharge assemblies 3 respectively. Therefore, during the cleaning and high-temperature sterilization process of any one sampling valve 2, the remaining sampling valves 2 can be used normally to complete the sampling work. The first branch pipe 512 is communicated with an external water source, and water flow can enter the first annular pipe 511 through the first branch pipe 512. When a certain sampling valve 2 is opened and the sampling is completed, the first electric control valve 515 at the corresponding position of the sampling valve 2 is opened. The water flow in the first annular pipe 511 enters the second branch pipe 514, then enters the second annular pipe 513, and finally enters the three liquid inlet channels 311 through the three third branch pipes 516 to complete the cleaning task, and then the first electric control valve 515 is closed.

[0054] Please refer to Figures 1-19 , the air inlet assembly 52 includes a third annular pipe 521, a fourth branch pipe 522, a fourth annular pipe 523, a fifth branch pipe 524, a second electric control valve 525 and three sixth branch pipes 526. The fourth branch pipe 522 is communicated with an external steam gas source, and high-pressure steam can enter the third annular pipe 521 through the fourth branch pipe 522. When the liquid inlet assembly 51 completes the cleaning of a certain sampling valve 2, the second electric control valve 525 at the corresponding position of the sampling valve 2 is opened. The high-pressure steam in the third annular pipe 521 enters the fifth branch pipe 524, then enters the fourth annular pipe 523, and finally enters the three air inlet channels 312 through the three sixth branch pipes 526 to complete the high-temperature sterilization task, and then the second electric control valve 525 is closed.

[0055] Please refer to Figures 1-22 , the waste recycling assembly 53 includes a recycling box 531, a cleaning pipe 532 and a second driving assembly 533. The recycling box 531 is arranged in the second assembly cavity 43. The cleaning pipe 532 has an L-shaped structure. An avoidance opening 44 is arranged at the bottom of the outer side wall of the assembly tank 4. The first end of the cleaning pipe 532 is arranged in the second opening 342, and the second end of the cleaning pipe 532 passes through the avoidance opening 44 and is fixedly connected to the middle of the side wall of the recycling box 531. The second driving assembly 533 includes an arc-shaped rack 5331, a second motor 5332 and a second gear 5333. When the second motor 5332 drives the second gear 5333 to rotate forward or backward, the arc-shaped rack 5331 can be driven to rotate around the axis of the assembly tank 4, and the arc-shaped rack 5331 drives the cleaning pipe 532 to change its position.

[0056] Please refer to Figures 1-22, when the cleaning pipe 532 leaves the second opening 342, the content of the fermentation tank 1 will be discharged through the second opening 342 to complete the sampling task. When the cleaning pipe 532 moves into the second opening 342, water flow or high-pressure steam will enter the cleaning pipe 532 through the second opening 342 and finally drain into the recovery box 531. When the high-pressure steam enters the recovery box 531, the accumulated water in the recovery box 531 has submerged the second end of the cleaning pipe 532. In this embodiment, the top surface of the recovery box 531 is open. When the high-pressure steam jets out from the second end of the cleaning pipe 532, it will enter the accumulated water and float away from the recovery box 531. During this process, the accumulated water in the recovery box 531 absorbs the heat of the high-pressure steam, and the steam leaving the recovery box 531 will enter the second assembly cavity 43 and finally leave the assembly tank 4 through the avoidance port 44.

[0057] Please refer to Figures 1-22 , the present invention further includes a sampling auxiliary component 6. The sampling auxiliary component 6 is arranged outside the assembly tank 4. The sampling auxiliary component 6 includes a first isolation cover 61, a second isolation cover 62, a material transfer pipe 64 and an arc-shaped rod 65. Receiving ports 63 are provided on the opposite side walls of the first isolation cover 61 and the second isolation cover 62. The first end of the arc-shaped rod 65 is detachably connected to the material transfer pipe 64, the second end of the arc-shaped rod 65 is fixedly connected to the cleaning pipe 532, and an annular slide rail 45 is sleeved on the outer side wall of the assembly tank 4. The arc-shaped rod 65 can slide along the annular slide rail 45.

[0058] Please refer to Figures 1-22 , when the present invention is not in use, the two ends of the material transfer pipe 64 are respectively arranged in the two receiving ports 63, and the first end of the cleaning pipe 532 is arranged in the second opening 342. The first isolation cover 61 and the second isolation cover 62 can ensure that the material transfer pipe 64 is not contaminated, and the cleaning pipe 532 ensures that the inside of the discharge guiding cover 34 is not contaminated.

[0059] Please refer to Figures 1-22 , when sampling is required, the second motor 5332 drives the second gear 5333 to rotate forward, the cleaning pipe 532 leaves the second opening 342, and the cleaning pipe 532 drives the recovery box 531 to change its position in the second assembly cavity 43. While the cleaning pipe 532 moves along the annular slide rail 45 through the arc-shaped rod 65, the arc-shaped rod 65 drives the material transfer pipe 64 to move below the discharge guiding cover 34, and the top of the material transfer pipe 64 enters the second opening 342. Subsequently, the sampling valve 2 is opened, and the content of the fermentation tank 1 will enter the material transfer pipe 64 through the second opening 342 and be discharged from the bottom of the material transfer pipe 64 to complete the sampling. The material transfer pipe 64 is a disposable item and can be removed and discarded after sampling.

[0060] Please refer to Figures 1-22After the sampling process is completed, the second motor 5332 drives the second gear 5333 to reverse, the cleaning pipe 532 re-enters the second opening 342, and the recycling box 531 returns to its original position within the second assembly cavity 43. At this time, the liquid inlet assembly 51 and the air inlet assembly 52 work in sequence to complete the tasks of cleaning and high-temperature sterilization. Finally, a new material transfer pipe 64 is installed on the arc-shaped rod 65 and waits for the next use.

[0061] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-described exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0062] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A fermentation tank sampling device for mycoplasma vaccine preparation, characterized in that: include: Fermentation tanks; A sampling valve is fixedly arranged on the side wall of the fermentation tank; A discharge assembly, the discharge assembly includes a discharge pipe, the first end of the discharge pipe is connected to the sampling valve, a liquid inlet channel and an air inlet channel are provided inside the discharge pipe, the first ends of the liquid inlet channel and the air inlet channel both pass through the inner surface of the discharge pipe, and the second ends of the liquid inlet channel and the air inlet channel both pass through the outer surface of the discharge pipe.

2. The fermenter sampling device for mycoplasma vaccine preparation according to claim 1, characterized in that: There are three liquid inlet channels and three air inlet channels respectively, and the liquid inlet channels and the air inlet channels are arranged at intervals; The outer surface of the fermentation tank is sleeved with an assembly tank, and a partition plate is provided inside the assembly tank, and the partition plate divides the interior of the fermentation tank into a first assembly chamber and a second assembly chamber, the first assembly chamber is located above the second assembly chamber, and the sampling valve and the discharge pipe are both located in the first assembly chamber; The discharging assembly further comprises: An adjusting ring is arranged on the inner side of the discharge pipe and is located near one end of the sampling valve. The adjusting ring is rotatably connected to the discharge pipe and is provided with three through holes distributed at equal intervals. An adjusting cylinder is arranged on the inner side of the discharge pipe and is located away from the end of the sampling valve. The adjusting cylinder can slide along the inner wall of the discharge pipe. The adjusting cylinder is fixedly provided with three adjusting blocks distributed at equal intervals at one end close to the adjusting ring. The inner side wall of the adjusting ring is provided with three adjusting grooves. The positions of the three adjusting blocks and the three adjusting grooves correspond to each other one by one. Each adjusting groove is provided with one of the through holes. A discharge guide cover is fixedly arranged on the outer side wall of the assembly tank and corresponds to the position of the discharge pipe. A first opening is arranged on the side wall of the discharge guide cover close to the discharge pipe, and a second opening is arranged at the bottom of the discharge guide cover. Both the first opening and the second opening are connected to the interior of the discharge guide cover.

3. The fermenter sampling device for preparing mycoplasma vaccine according to claim 2, characterized in that: The cross-sectional shape of the adjusting block is a triangle, the shape of the adjusting groove matches the shape of the adjusting block, and the adjusting block can slide along the inner wall of the adjusting groove; A plurality of metal plates distributed at equal intervals are fixedly arranged on the inner side wall of the discharge pipe close to one end of the adjustment ring, and a plurality of magnets distributed at equal intervals are fixedly arranged on the outer side wall of the adjustment ring; The end of the adjusting tube away from the adjusting ring is provided with three transmission ports distributed at equal intervals, one of which is located at the top of the adjusting tube and has an opening area larger than the other two transmission ports; The discharge assembly further includes a first drive assembly, an annular assembly groove is provided on the inner side wall of the discharge pipe, the annular assembly groove is located at one end away from the adjustment ring, the first drive assembly is arranged on the inner side of the annular assembly groove, and the first drive assembly includes: An annular rack, sleeved on the outer surface of the adjusting cylinder and fixedly connected to the adjusting cylinder; An electric telescopic rod, horizontally arranged above the adjusting cylinder and fixedly connected to the discharge pipe; An arc frame is provided between the annular rack and the electric telescopic rod, the arc frame is sleeved on the outer surface of the adjusting cylinder and can slide along the outer surface of the adjusting cylinder, the arc frame is fixedly connected to the output end of the electric telescopic rod, a limit block is provided between the annular rack and the arc frame, a guide groove is provided on the side wall of the arc frame, a first end of the limit block is fixedly connected to the annular rack, a second end of the limit block extends into the guide groove and can slide along the guide groove, and a width of the second end of the limit block is greater than a width of the first end of the limit block; The first motor is fixedly arranged on the arc frame, the output end of the first motor is fixedly connected to the first gear, and the first gear is meshed with the annular rack for transmission.

4. The fermenter sampling device for preparing mycoplasma vaccine according to claim 2, characterized in that: Also included is a flushing and disinfection component, the flushing and disinfection component comprising: A liquid inlet assembly, disposed in the first assembly cavity, and used for delivering flushing liquid to the liquid inlet channel; An air intake component is arranged in the first assembly cavity and is used for conveying high-pressure steam to the air intake passage.

5. The fermenter sampling device for mycoplasma vaccine preparation according to claim 4, characterized in that: The liquid inlet assembly comprises: A first annular tube, wherein a first branch tube is disposed on the top of the first annular tube, a first end of the first branch tube is communicated with the interior of the first annular tube, and a second end of the first branch tube extends to the outside of the assembly tank; A second annular tube is sleeved on the outer side of the discharge tube, a second branch tube is provided between the second annular tube and the first annular tube, two ends of the second branch tube are respectively connected with the interior of the first annular tube and the second annular tube, a first electrically-controlled valve is provided in the middle of the second branch tube, three third branch tubes are provided between the second annular tube and the discharge tube, the first ends of the three third branch tubes are all connected with the interior of the second annular tube, and the second ends of the three third branch tubes are respectively connected with the second ends of the three liquid inlet channels.

6. The fermenter sampling device for preparing mycoplasma vaccine according to claim 4, characterized in that: The air intake assembly comprises: a third annular tube, wherein a fourth branch tube is disposed on the top of the third annular tube, a first end of the fourth branch tube is communicated with the interior of the third annular tube, and a second end of the fourth branch tube extends to the outside of the assembly tank; A fourth annular tube is sleeved on the outer side of the discharge tube, a fifth branch tube is provided between the fourth annular tube and the third annular tube, two ends of the fifth branch tube are respectively connected with the interior of the third annular tube and the fourth annular tube, a second electrically-controlled valve is provided in the middle of the fifth branch tube, three sixth branch tubes are provided between the fourth annular tube and the discharge tube, the first ends of the three sixth branch tubes are all connected with the interior of the fourth annular tube, and the second ends of the three sixth branch tubes are respectively connected with the second ends of the three intake passages.

7. The fermenter sampling device for mycoplasma vaccine preparation according to claim 4, characterized in that: The flushing and disinfection assembly also includes a waste recovery assembly, and the waste recovery assembly includes: A recovery box is disposed in the second assembly cavity and is capable of sliding along the inner wall of the second assembly cavity, and the top surface of the recovery box is open; A cleaning pipe is arranged on the outside of the assembly tank, the cleaning pipe is in an L-shaped structure, the second opening passes through the side walls of the discharge guide cover on both sides, and a avoidance opening is provided at the bottom of the outer wall of the assembly tank. The first end of the cleaning pipe is arranged in the second opening and can slide along the inner wall of the second opening. The second end of the cleaning pipe passes through the avoidance opening and is fixedly connected to the middle part of the side wall of the recovery box, and the second end of the cleaning pipe is communicated with the interior of the recovery box; The second drive assembly is arranged on the outside of the assembly tank. The second drive assembly includes an arc-shaped rack. The first end of the arc-shaped rack is fixedly connected to the cleaning tube. A second motor is fixedly arranged on the outer wall of the assembly tank. The output end of the second motor is fixedly connected to the second gear. The second gear is meshed with the arc-shaped rack for transmission.

8. The fermenter sampling device for preparing mycoplasma vaccine according to claim 7, characterized in that: It also includes a sampling auxiliary component, which is arranged on the outside of the assembly tank and includes: A first isolation cover is fixedly disposed on the outer side wall of the assembly tank and is located on a side of the cleaning pipe away from the second motor; A second isolation cover is arranged directly below the first isolation cover and is fixedly connected to the outer side wall of the assembly tank, and the first isolation cover and the second isolation cover are provided with receiving openings on the opposite side walls; A material transfer pipe is vertically arranged between the first isolation cover and the second isolation cover, and two ends of the material transfer pipe are respectively arranged in the two receiving openings; An arc-shaped rod, wherein the first end of the arc-shaped rod is detachably connected to the material transfer tube, the second end of the arc-shaped rod is fixedly connected to the cleaning tube, and an annular slide rail is sleeved on the outer wall of the assembly tank, and the arc-shaped rod can slide along the annular slide rail.

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