Bioactive peptide assembly gel dressing as well as preparation method and application thereof

Abstract

The invention relates to the technical field of peptide self-assembly long-acting skin care, and discloses a bioactive peptide assembly gel dressing as well as a preparation method and application thereof. The invention relates to a preparation method of a bioactive peptide assembly gel dressing. The preparation method comprises the following steps: 1) preparing a palmitoyl tripeptide-5 bioactive peptide assembly; 2) preparing a gelatin carrier; and (3) adding the palmitoyl tripeptide-5 active peptide assembly obtained in the step (1) into the gelatin carrier obtained in the step (2), uniformly mixing, and standing to obtain the bioactive peptide assembly gel dressing. The bioactive peptide assembly gel dressing provided by the invention has a long-acting skin care function.

Description

Technical Field

[0001] The present invention relates to the technical field of long-acting skin care by peptide self-assembly, and more specifically, to a bioactive peptide assembly gel dressing, a preparation method thereof, and an application thereof. Background Art

[0002] Skin aging is a complex biological process affected by multiple factors, including internal genetic factors and external environmental factors. These factors act together, resulting in the loss of collagen and extracellular matrix in the skin. To effectively combat skin aging, people have started to seek active ingredients with safe components and clear mechanisms of action to achieve ideal anti-aging effects. Among many active ingredients, bioactive peptides are widely used in anti-aging products because they can regulate biological metabolic processes related to skin aging. Among them, palmitoyl tripeptide-5 is the most widely used bioactive peptide. Palmitoyl tripeptide-5 belongs to the signal peptide type of bioactive peptides and is an analogue of thrombospondin-1 (TSP-1). It can promote the activities of stromal cells, up-regulate transforming growth factor-β (TGF-β), and promote the synthesis of collagen (type I, type II, and type IV). In addition, palmitoyl tripeptide-5 also has the characteristics of enhancing cell activity, improving skin luster, and improving skin color. Therefore, many cosmetic brands use palmitoyl tripeptide-5 as a nutritional anti-wrinkle raw material.

[0003] However, small molecule palmitoyl tripeptide-5-based bioactive peptides have short-term skin care effects and lack long-acting skin care effects. Therefore, the preparation of palmitoyl tripeptide-5-based bioactive peptide cosmetics with long-acting skin care functions has become the focus of attention of those skilled in the art. Studies have shown that the polypeptide self-assembly technology can connect polypeptide molecules into specific peptide assembly structures through non-covalent bonds such as hydrophobic interactions, hydrogen bonds, and van der Waals forces. This peptide assembly structure can continuously release active peptide molecules in the skin environment through the "assembly-disassembly equilibrium", thereby achieving long-acting skin care effects. Therefore, constructing a palmitoyl tripeptide-5 assembly can realize the preparation of palmitoyl tripeptide-5-based bioactive peptide cosmetics with long-acting skin care functions. Summary of the Invention

[0004] The purpose of the present invention is to provide a collagen hydrogel beauty mask with a self-assembled bioactive peptide as a skin care peptide activity library

[0005] To achieve the above purpose, the present invention provides the following technical solutions:

[0006] One of the technical solutions of the present invention:

[0007] A preparation method of a bioactive peptide assembly gel dressing, comprising the following steps:

[0008] 1) Prepare a palmitoyl tripeptide-5 active peptide assembly;

[0009] 2) Prepare a gelatin carrier;

[0010] 3) Add the palmitoyl tripeptide-5 active peptide assembly obtained in step 1 to the gelatin carrier obtained in step 2, mix evenly, and let stand to obtain the bioactive peptide assembly gel dressing.

[0011] Further, in step 1), the preparation of the palmitoyl tripeptide-5 active peptide assembly specifically includes the following steps:

[0012] Place palmitoyl tripeptide-5 in ultrapure water, disperse it by ultrasonic wave to obtain a palmitoyl tripeptide-5 solution, and then adjust the pH of the palmitoyl tripeptide-5 solution to 7.5-8.0, and let it self-assemble by standing to obtain the palmitoyl tripeptide-5 active peptide assembly.

[0013] Furthermore, the concentration of the palmitoyl tripeptide-5 solution is 0.2-1.8% (w / v).

[0014] Furthermore, the duration of ultrasonic dispersion is 30 min.

[0015] Furthermore, the pH adjustment specifically is: use a NaOH solution with a concentration of 0.5 mol·L -1 to adjust the pH of the palmitoyl tripeptide-5 solution.

[0016] Further, in step 2), the preparation of the gelatin carrier specifically includes the following steps:

[0017] Place fish skin gelatin in ultrapure water, let it stand, and then heat it with stirring in a water bath to obtain the gelatin carrier.

[0018] Furthermore, the concentration of the gelatin carrier is 1.4% (w / v).

[0019] Furthermore, the standing specifically is: let it stand at room temperature for 2 h.

[0020] Furthermore, the heating with stirring in a water bath specifically is: heat it with stirring in a water bath at 70 °C for 15 min.

[0021] Furthermore, after the heating with stirring in a water bath, it is also necessary to cool naturally to 30 °C at room temperature.

[0022] Further, in step 3), the mass ratio of the palmitoyl tripeptide-5 active peptide assembly to the gelatin carrier is 1:10000.

[0023] Further, in step 3), the standing specifically is: let it stand at 8-12 °C for 16-18 h.

[0024] The second technical solution of the present invention:

[0025] The bioactive peptide assembly gel dressing prepared by the preparation method of the above-mentioned bioactive peptide assembly gel dressing.

[0026] The third technical solution of the present invention:

[0027] The application of the above bioactive peptide assembly gel dressing in the field of cosmetics.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] The bioactive peptide assembly gel dressing provided by the present invention selects palmitoyl tripeptide-5, a bioactive peptide molecule with the most extensive application and anti-wrinkle skin care effect, as the raw material. By virtue of its unique molecular structure, it self-assembles into a palmitoyl tripeptide-5 active peptide assembly, forms a skin care peptide active molecule library, and loads this skin care peptide active molecule library into a high water-locking gelatin carrier to prepare a bioactive peptide assembly gel dressing with long-term skin care function;

[0030] The preparation method of the bioactive peptide assembly gel dressing provided by the present invention is simple to operate and has high safety, which is of great significance for the development of the field of bioactive peptide self-assembly skin care;

[0031] The palmitoyl tripeptide-5 used in the present invention has a molecular structure composed of a hydrophobic C16 alkyl chain and three hydrophilic amino acids (lysine-histidine-lysine) active skin care parts. After entering skin cells, it promotes the generation of cell collagen through a series of signal transduction effects, smooths wrinkles, and achieves precise skin care effects; importantly, the long alkyl chain in palmitoyl tripeptide-5 can promote the formation of an active peptide molecule library containing a large number of active peptide molecule assembly structures, and continuously release active peptide molecules by using the "assembly-disassembly balance" to play a long-term skin care role;

[0032] The collagen hydrogel carrier used in the present invention is prepared from gelatin. Gelatin is a partial hydrolysis product of collagen and has good biocompatibility; asparagine, glutamine and proline in the gelatin hydrogel film enhance the natural generation of elastin, provide energy for cells and maintain the correct water balance inside cells, and have the ability to tighten and smooth the skin; the triple-stranded helical structure of gelatin can wrap a large number of water molecules to form a gel to play a role in moisturizing the skin; in addition, it also has sticky and tearable properties, which can remove excess oil on the skin surface and play a role in preventing inflammatory acne. Description of the drawings

[0033] By reading the following detailed description of the preferred embodiments, various other advantages and benefits will become clear to those of ordinary skill in the art. The drawings are only for the purpose of showing the preferred embodiments and are not considered to be a limitation of the present invention. Also, throughout the drawings, the same reference numerals are used to represent the same components. In the drawings:

[0034] Figure 1 It is the morphological structure diagram of the palmitoyl tripeptide-5 active peptide assembly prepared in step 1 of Example 1;

[0035] Figure 2 It is the detection result of the dispersion form of palmitoyl tripeptide-5 in the bioactive peptide assembly gel dressing. Among them, A is the palmitoyl tripeptide-5-FITC fluorescence conjugate, B is the FITC-labeled palmitoyl tripeptide-5 active peptide assembly, and C is the FITC-labeled bioactive peptide assembly gel dressing;

[0036] Figure 3 It is the detection result of the uptake of palmitoyl tripeptide-5 by fibroblasts;

[0037] Figure 4 It is the detection result of the long-acting transdermal release performance of the palmitoyl tripeptide-5 active peptide assembly and the bioactive peptide assembly gel dressing prepared in Example 1;

[0038] Figure 5 It is the detection result of the water loss rate of the bioactive peptide assembly gel dressing prepared in Example 1;

[0039] Figure 6 It is the state diagram at different times after the bioactive peptide assembly gel dressing prepared in Example 1 is applied to the arm fold. Among them, A is 0 min, B is 6 h, and C is 12 h;

[0040] Figure 7 It is the skin state diagram of the arm fold before and after the bioactive peptide assembly gel dressing prepared in Example 1. Among them, A is before use and B is after use;

[0041] Figure 8 It is the detection result of the facial skin state before and after the bioactive peptide assembly gel dressing prepared in Example 1;

[0042] Figure 9 It is the detection result of the facial skin state before and after the bioactive peptide assembly gel dressing prepared in Example 1. Specific Embodiments

[0043] The various exemplary embodiments of the present invention will be described in detail below. This detailed description should not be considered as a limitation on the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention. It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention.

[0044] In addition, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.

[0045] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the said documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0046] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the present invention specification, which are obvious to those skilled in the art. Other embodiments obtained from the specification of the present invention are obvious to those skilled in the art. The specification and examples of the present invention are merely exemplary.

[0047] Regarding the use of "comprising", "including", "having", "containing", etc. herein, they are all open-ended terms, meaning including but not limited to.

[0048] In the following examples, all raw materials used were commercially purchased. Among them, palmitoyl tripeptide-5 was purchased from Chengdu Yunxi Chemical Co., Ltd., and the purity was 99.33%.

[0049] For a bioactive peptide assembly gel dressing provided by the present invention, when preparing the palmitoyl tripeptide-5 active peptide assembly, the preferred concentration of palmitoyl tripeptide-5:

[0050] Palmitoyl tripeptide-5 was placed in ultrapure water at concentrations of 0.2% (w / v), 0.6% (w / v), 1.0% (w / v), 1.4% (w / v), and 1.8% (w / v) respectively, and ultrasonically dispersed for 30 min to obtain 5 palmitoyl tripeptide-5 solutions. Then, a solution with a concentration of 0.5 mol·L -1NaOH solution until the pH of the palmitoyl tripeptide-5 solution reaches 7.5, and let it stand for self-assembly at room temperature to obtain 5 kinds of palmitoyl tripeptide-5 active peptide assemblies;

[0051] Observe the states of the 5 obtained palmitoyl tripeptide-5 active peptide assemblies. Among them, when the concentrations are 0.2% (w / v) and 0.6% (w / v), the palmitoyl tripeptide-5 active peptide assemblies show a solution state. When the concentrations are 1.0% (w / v) and 1.4% (w / v), the palmitoyl tripeptide-5 active peptide assemblies show a solid-liquid coexistence state. When the concentration is 1.8% (w / v), the palmitoyl tripeptide-5 active peptide assemblies show a gel solid state. Therefore, it is preferred to prepare the palmitoyl tripeptide-5 active peptide assemblies at a concentration of 1.8% (w / v).

[0052] Example 1

[0053] A bioactive peptide assembly gel dressing

[0054] 1. Preparation of palmitoyl tripeptide-5 active peptide assemblies

[0055] According to a concentration of 1.8% (w / v), place palmitoyl tripeptide-5 in ultrapure water, ultrasonically disperse for 30 min to obtain a palmitoyl tripeptide-5 solution. Then, add a 0.5 mol·L -1 NaOH solution until the pH of the palmitoyl tripeptide-5 solution reaches 7.5, and let it stand for self-assembly at room temperature to obtain palmitoyl tripeptide-5 active peptide assemblies;

[0056] The morphological structure of the palmitoyl tripeptide-5 active peptide assemblies prepared in step 1 of Example 1 is as Figure 1 shown;

[0057] From Figure 1 it can be seen that the palmitoyl tripeptide-5 active peptide assemblies are long ribbon helix structures, the ribbon helix length is about 4 μm, and the ribbon helix height is about 60 nm.

[0058] 2. Preparation of gelatin carrier

[0059] According to a concentration of 1.4% (w / v), place fish skin gelatin in ultrapure water, let it stand at room temperature for 2 h, then heat it with stirring in a water bath at 70 °C for 15 min, and naturally cool it to 30 °C at room temperature to obtain a gelatin carrier;

[0060] 3. Preparation of bioactive peptide assembly gel dressing

[0061] According to the mass ratio of the palmitoyl tripeptide-5 active peptide assembly to the gelatin carrier being 1:10,000, the palmitoyl tripeptide-5 active peptide assembly obtained in step 1 was added to the gelatin carrier obtained in step 2, mixed evenly, and left to stand at 10 °C for 17 h to obtain the bioactive peptide assembly gel dressing.

[0062] Effect verification

[0063] I. Dispersion form of palmitoyl tripeptide-5 in the bioactive peptide assembly gel dressing

[0064] The dispersion form of palmitoyl tripeptide-5 in the bioactive peptide assembly gel dressing was detected. The specific detection method was as follows:

[0065] 1) Palmitoyl tripeptide-5 was placed in a NaHCO3 solution with a concentration of 0.1 mol·L -1 and ultrasonically dispersed for 30 min to obtain a palmitoyl tripeptide-5 solution;

[0066] 2) According to the volume ratio of the palmitoyl tripeptide-5 solution to the FITC solution (fluorescein isothiocyanate) being 1 mL:50 μL, a 3-fold concentration of the FITC solution was added to the palmitoyl tripeptide-5 solution prepared in step 1), stirred and mixed at room temperature for 4 h, and left overnight in a 4 °C refrigerator to obtain a palmitoyl tripeptide-5-FITC fluorescent conjugate;

[0067] 3) According to the final concentration of NH4Cl being 50 mol·L -1 , the NH4Cl solution was added to the palmitoyl tripeptide-5-FITC fluorescent conjugate obtained in step 2), stirred and reacted at room temperature for 2 h, and centrifuged at 12,000×g for 10 min to remove the excess FITC in the palmitoyl tripeptide-5-FITC fluorescent conjugate;

[0068] 4) Using the palmitoyl tripeptide-5-FITC fluorescent conjugate obtained in step 3) as the raw material, referring to Example 1 to prepare the bioactive peptide assembly gel dressing, and then observing the prepared bioactive peptide assembly gel dressing using an inverted fluorescence microscope;

[0069] The detection results of the dispersion form of palmitoyl tripeptide-5 in the bioactive peptide assembly gel dressing are as Figure 2 shown, where A is the palmitoyl tripeptide-5-FITC fluorescent conjugate, B is the FITC-labeled palmitoyl tripeptide-5 active peptide assembly, and C is the FITC-labeled bioactive peptide assembly gel dressing;

[0070] From Figure 2It can be seen that the inverted fluorescence microscope image of the palmitoyl tripeptide-5-FITC fluorescent conjugate does not show green fluorescence, while the inverted fluorescence microscope images of the FITC-labeled palmitoyl tripeptide-5 active peptide assembly and the FITC-labeled bioactive peptide assembly gel dressing show green fluorescence, indicating that palmitoyl tripeptide-5 in the bioactive peptide assembly gel dressing is dispersed in the form of palmitoyl tripeptide-5 active peptide assembly.

[0071] II. Fibroblast Uptake Experiment

[0072] Place the above-prepared FITC-labeled bioactive peptide assembly gel dressing in fibroblast medium (fibroblast concentration is 10 5 cells / mL), incubate with fibroblasts, aspirate the medium, and rinse the cells with 1×PBS at least 3 times; add 400 μL of 4% paraformaldehyde solution to each well, fix at 37 °C for 15 min, wash the cells with 1×PBS 2 - 3 times, add 400 μL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride) to each well to stain the cell nuclei, remove the staining solution after 15 min, wash the cells with 1×PBS 2 - 3 times, and then observe the uptake of palmitoyl tripeptide-5 by fibroblasts at 5 min, 30 min, and 120 min respectively through an inverted fluorescence microscope;

[0073] The detection results of the uptake of palmitoyl tripeptide-5 by fibroblasts are as Figure 3 shown;

[0074] As Figure 3 can be seen, with the prolongation of the uptake time, the uptake of palmitoyl tripeptide-5 by fibroblasts gradually increases; moreover, only after co-incubation for 5 min, fibroblasts have taken up a small amount of palmitoyl tripeptide-5, indicating that the palmitoyl tripeptide-5 active peptide assembly can effectively release palmitoyl tripeptide-5 into fibroblasts to play a role, and the uptake of palmitoyl tripeptide-5 by fibroblasts shows time dependence.

[0075] III. In Vitro Skin Penetration Study

[0076] Detect the long-acting transdermal release performance of the palmitoyl tripeptide-5 active peptide assembly and the bioactive peptide assembly gel dressing prepared in Example 1. The specific detection method is as follows:

[0077] Add 30 mL of normal saline to the diffusion cell as the release solution, then fix the pig skin at one end of the diffusion cell with the skin surface facing the donor chamber;

[0078] In the donor chamber, evenly apply 3 g of the palmitoyl tripeptide-5 active peptide assembly or the bioactive peptide assembly gel dressing prepared in Example 1 on 3 cm -2On the surface of pigskin, at 32 °C, the stirring speed was controlled at 200 r·min -1 , and the release solution was stirred;

[0079] Samples of the release solution were taken at 30 min, 1 h, 2 h, 6 h, 12 h, 24 h, 36 h, and 48 h respectively, and the characteristic absorption peak of palmitoyl tripeptide-5 was detected by ultraviolet absorption spectroscopy. Referring to the standard curve Y = 0.146X + 0.203, r 2 = 0.99, the concentration of palmitoyl tripeptide-5 in the release solution was detected;

[0080] The transdermal rate was calculated with reference to the following formula;

[0081] Transdermal rate = (mass of palmitoyl tripeptide-5 in the release solution / mass of palmitoyl tripeptide-5 active peptide assembly or bioactive peptide assembly gel dressing) × 100%.

[0082] The detection results of the long-acting transdermal release performance of the palmitoyl tripeptide-5 active peptide assembly and the bioactive peptide assembly gel dressing prepared in Example 1 are as Figure 4 shown;

[0083] It can be Figure 4 seen that the transdermal rate of the palmitoyl tripeptide-5 active peptide assembly within 24 h was 94%, and the transdermal rate of the bioactive peptide assembly gel dressing within 24 h was 78%. Therefore, the bioactive peptide assembly gel dressing effectively slowed down the transdermal rate of palmitoyl tripeptide-5, had a long-acting transdermal duration of 48 h, and the transdermal time was extended by 2 times;

[0084] The reason why the bioactive peptide assembly gel dressing has a long-acting transdermal duration lies in the use of the gelatin carrier; the use of the gelatin carrier avoids the sudden release of palmitoyl tripeptide-5 in the initial stage, promotes the gradual disassembly of the palmitoyl tripeptide-5 active peptide assembly, achieves a slow-release effect, and thus realizes a long-acting skin care effect.

[0085] IV. Water content change experiment

[0086] The in vitro water loss rate of the bioactive peptide assembly gel dressing prepared in Example 1 was detected. The specific detection method was as follows:

[0087] The bioactive peptide assembly gel dressing prepared in Example 1 was placed in a room temperature environment, and then the bioactive peptide assembly gel dressing was weighed at 0 min, 5 min, 30 min, 1 h, 2 h, 4 h, and 12 h respectively, and the water loss rate was calculated;

[0088] Water loss rate = (initial weight - weight after placement) / initial weight × 100%

[0089] The water loss rate test results of the bioactive peptide assembly gel dressing prepared in Example 1 are as follows Figure 5 shown;

[0090] It can be seen from Figure 5 that after the bioactive peptide assembly gel dressing is placed for 1 h, the water loss rate is only 8.5%, far lower than that of existing dressings; and after being placed for 12 h, the water loss rate is only 54.6%, indicating that the bioactive peptide assembly gel dressing prepared in the present invention has an ultra-long water-locking and moisturizing ability;

[0091] The reason why the bioactive peptide assembly gel dressing has an ultra-long water-locking and moisturizing ability lies in the use of a gelatin carrier; gelatin molecules are partial hydrolysis products of collagen and are helices formed by the intertwining of three polypeptide chains, containing a large number of hydroxyl groups, carboxyl groups and amino groups. These functional groups endow gelatin with extremely strong hydrophilicity, and it is difficult for water molecules to escape from the structure.

[0092] V. Usage effect

[0093] The bioactive peptide assembly gel dressing prepared in Example 1 was evenly applied to the arm folds, and then the state of the bioactive peptide assembly gel dressing and the skin state of the arm folds before and after using the bioactive peptide assembly gel dressing prepared in Example 1 were observed at 0 h, 6 h and 12 h respectively;

[0094] The states of the bioactive peptide assembly gel dressing prepared in Example 1 at different times after being applied to the arm folds are as Figure 6 shown, where A is 0 min, B is 6 h, and C is 12 h;

[0095] It can be seen from Figure 6 that at 0 min, the bioactive peptide assembly gel dressing is in a transparent state, at 6 h, the bioactive peptide assembly gel dressing gradually melts into a liquid, and at 12 h, the palmitoyl tripeptide-5 and water in the bioactive peptide assembly gel dressing are absorbed, presenting a dry film state;

[0096] The skin states of the arm folds before and after using the bioactive peptide assembly gel dressing prepared in Example 1 are as Figure 7 shown, where A is before use and B is after use;

[0097] It can be seen from Figure 7 that after use, the arm folds at the arm folds are significantly reduced and become tender.

[0098] The bioactive peptide assembly gel dressing prepared in Example 1 was evenly applied to the face for 12 h, and then the skin state of the face before and after using the bioactive peptide assembly gel dressing prepared in Example 1 was detected by a SKINLSVEN facial skin detection instrument;

[0099] The detection results of the front and back facial skin conditions using the bioactive peptide assembly gel dressing prepared in Example 1 are as Figure 8 and Figure 9 shown;

[0100] From Figure 8 and Figure 9 it can be seen that after using the bioactive peptide assembly gel dressing prepared in Example 1, the facial pores, roughness, and wrinkles are all reduced, and the surface moisture content is relatively increased, indicating that the bioactive peptide assembly gel dressing prepared in Example 1 has good skin care effects.

[0101] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the above embodiments, those of ordinary skill in the art should understand that: the specific implementation manners of the present invention can still be modified or equivalently replaced, and any modification or equivalent replacement without departing from the spirit and scope of the present invention shall be covered by the protection scope of the claims of the present invention.

Claims

1. A preparation method of a bioactive peptide assembly gel dressing, characterized in that, It includes the following steps: 1) Prepare a palmitoyl tripeptide-5 active peptide assembly; 2) Prepare a gelatin carrier; 3) Add the palmitoyl tripeptide-5 active peptide assembly obtained in step 1 to the gelatin carrier obtained in step 2, mix evenly, and let stand to obtain the bioactive peptide assembly gel dressing.

2. The preparation method of a bioactive peptide assembly gel dressing according to claim 1, characterized in that, In step 1), the preparation of the palmitoyl tripeptide-5 active peptide assembly specifically includes the following steps: Place palmitoyl tripeptide-5 in ultrapure water, disperse it by ultrasonic treatment to obtain a palmitoyl tripeptide-5 solution, and then adjust the pH of the palmitoyl tripeptide-5 solution to 7.5-8.0, and let it stand for self-assembly to obtain the palmitoyl tripeptide-5 active peptide assembly.

3. The preparation method of a bioactive peptide assembly gel dressing according to claim 2, characterized in that, The concentration of the palmitoyl tripeptide-5 solution is 0.2 to 1.8%; the duration of ultrasonic dispersion is 30 min; specifically, the pH adjustment is as follows: using a NaOH solution with a concentration of 0.5 mol·L -1 to adjust the pH of the palmitoyl tripeptide-5 solution.

4. The preparation method of a bioactive peptide assembly gel dressing according to claim 1, characterized in that, In step 2), the preparation of the gelatin carrier specifically includes the following steps: Place fish skin gelatin in ultrapure water, let it stand, and then heat it with stirring in a water bath to obtain the gelatin carrier.

5. The preparation method of a bioactive peptide assembly gel dressing according to claim 4, characterized in that, The concentration of the gelatin carrier is 1.4%; the standing specifically means: standing at room temperature for 2 h; the heating with stirring in a water bath specifically means: heating with stirring in a water bath at 70 °C for 15 min; after the heating with stirring in a water bath, it is also necessary to cool naturally to 30 °C at room temperature.

6. The preparation method of a bioactive peptide assembly gel dressing according to claim 1, characterized in that, In step 3), the mass ratio of the palmitoyl tripeptide-5 active peptide assembly to the gelatin carrier is 1:10000; the standing specifically means: standing at 8-12 °C for 16-18 h.

7. A bioactive peptide assembly gel dressing prepared by the preparation method of the bioactive peptide assembly gel dressing according to any one of claims 1-6.

8. An application of the bioactive peptide assembly gel dressing according to claim 7 in the field of cosmetics.

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