Fungaltoxin degradation agent and preparation method thereof

Abstract

The invention discloses a mycotoxin degradation agent and a preparation method thereof. The mycotoxin degrading agent is prepared from 10 to 20 parts of quinoa peptide, 30 to 40 parts of microcrystalline cellulose, 10 to 30 parts of modified alpha-lipoic acid, 10 to 20 parts of enzyme-containing preparation and 20 to 40 parts of L-ascorbic acid. The invention also provides a preparation method of the composition. Compared with the prior art, vomitoxin can be better degraded, the stress reaction of vomitoxin to piglets is reduced, the nutrient absorption of the feed is promoted, and the growth efficiency of animals is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of detoxification, and particularly to a mycotoxin degrading agent and a preparation method thereof. Background Art

[0002] Mycotoxins are toxic secondary metabolites produced by fungi during their growth and reproduction. They are widely present in food crops such as wheat, rice, and corn in nature. Currently, the mycotoxins that pose a greater threat to world food security include aflatoxins, ochratoxin, vomitoxin, and fumonisin, etc. Vomitoxin, also known as deoxynivalenol (DON), is a mycotoxin produced by certain fungi of the genus Fusarium. DON is the mycotoxin with the highest detection rate among all mycotoxins and is usually present in wheat, barley, and corn. Consuming food contaminated with vomitoxin can cause symptoms such as diarrhea, vomiting, and gastrointestinal diseases. Vomitoxin has the characteristics of being acid-resistant and heat-resistant, and it is difficult to completely remove by traditional cooking and processing methods. Commonly used removal methods include physical degradation, chemical degradation, biological degradation, and synergistic degradation of multiple methods. Adopting effective removal technologies can significantly reduce the contamination of mycotoxins in crops and ensure food safety.

[0003] DON is a mycotoxin with relatively weak lethality, but its effect of inhibiting animal growth is equivalent to or even higher than that of other trichothecenes. Before DON is orally ingested into the small intestine of ruminants or poultry, it will contact a large number of microorganisms, and the microorganisms will convert DON into low-toxic DOM-1. Therefore, they are very insensitive to DON. The intestinal microorganisms of humans and pigs mainly exist in the hindgut. Most of the DON has been absorbed in the foregut, and only a small part of the DON that has not been absorbed in the foregut reaches the hindgut and is converted into DOM-1. Therefore, pigs and humans are more sensitive to DON. Piglets are more sensitive to DON. Consuming food contaminated with vomitoxin will cause symptoms such as diarrhea, vomiting, anorexia, and gastrointestinal diseases in piglets, inhibit their growth, and thus affect weight gain. Therefore, it is necessary to provide a mycotoxin degrading agent to reduce the impact of DON on piglets.

[0004] CN113383860A discloses a vomitoxin antidote and a preparation process. The vomitoxin antidote is composed of Bacillus subtilis, vitamin C, montmorillonite, yeast cell wall, plant extract, and zinc methionine. The vomitoxin antidote provided by this invention has an obvious effect on vomitoxin and can improve the feed conversion rate at the same time, which has good application value for the feed and breeding industries. This invention mainly adsorbs vomitoxin through the adsorption of montmorillonite, and the absorption upper limit and the effect on vomitoxin may be relatively average.

[0005] CN116004449A discloses a Lactococcus lactis, a bacterial agent, a mycotoxin degrading agent and their applications, and a method for degrading mycotoxins. The Lactococcus lactis in this invention can efficiently degrade various mycotoxins. Using Lactococcus lactis as a biological material for degrading mycotoxins has good application prospects in developing new mycotoxin biodegradable bacterial agents and sterile preparations. The degrading agent prepared in this invention may have problems such as relatively high preparation costs. Summary of the Invention

[0006] In view of the above-mentioned defects of the prior art, the technical problem to be solved by this invention is to provide a mycotoxin degrading agent that can better degrade vomitoxin, reduce its stress response to piglets, promote feed nutrient absorption, and improve animal growth efficiency.

[0007] To achieve the above object, this invention provides a preparation method of a mycotoxin degrading agent. By mass: it is obtained by mixing 10 - 20 parts of quinoa peptide, 30 - 40 parts of microcrystalline cellulose, 10 - 30 parts of α-lipoic acid, 10 - 20 parts of enzyme-containing preparation, and 20 - 40 parts of L-ascorbic acid and passing through a sieve;

[0008] or it is obtained by mixing 10 - 20 parts of quinoa peptide, 30 - 40 parts of microcrystalline cellulose, 10 - 30 parts of modified α-lipoic acid, 10 - 20 parts of enzyme-containing preparation, and 20 - 40 parts of L-ascorbic acid and passing through a sieve;

[0009] Among them, the modified α-lipoic acid is α-lipoic acid modified by a natural cationic polymer.

[0010] Preferably, 1 - 10 parts of natural cationic polymer is mixed evenly with 80 - 120 parts of solvent, then 1 - 3 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.1 - 1 part of N-hydroxysuccinimide are added to obtain a mixed solution 1; 1 - 5 parts of α-lipoic acid is mixed evenly with 10 - 20 parts of absolute ethanol, added to the mixed solution 1, reacted at 30 - 50 °C and 800 - 1200 rpm for 20 - 28 h, cooled to room temperature, 150 - 250 parts of 70 wt% ethanol aqueous solution is added, stirred at 800 - 1200 rpm for 5 - 15 min, left to stand, centrifuged at 1000 - 3000 rpm for 3 - 8 min, the precipitate is taken, 10 - 25 parts of water is added, and then freeze-dried for 20 - 28 h to obtain the modified α-lipoic acid;

[0011] Further preferably, the solvent is water or 0.5 - 2.0 wt% acetic acid aqueous solution.

[0012] Further preferably, the natural cationic polymer is selected from at least one of chitosan, chitosan oligosaccharide, and polylysine.

[0013] As a further illustration of the present invention, lipoic acid contains carboxyl groups and disulfide bonds, and chitosan, chitosan oligosaccharide, and polylysine all contain amino groups and hydroxyl groups in their structures. The two may undergo amidation reactions, esterification reactions, disulfide bond exchange reactions, or hydrogen bond interaction reactions to produce functional groups such as amide bonds and hydrogen bonds to form copolymers, thereby preparing modified lipoic acid. The obtained modified lipoic acid has good stability in both acidic and alkaline environments. The present invention discovers that introducing modified lipoic acid into mycotoxin degrading agents may form a special structure that can kill Fusarium fungi, prevent the production of new toxins, and the active functional groups contained may react with the weakly acidic hydroxyl molecules on DON molecules or interact through hydrogen bonds to degrade or adsorb DON; it can also enhance the intestinal physical barrier, reduce the penetration of DON and its absorption by the intestine, scavenge free radicals, reduce the production of pro-inflammatory factors, reduce the diarrhea rate of piglets, promote feed nutrient absorption, and improve animal growth efficiency.

[0014] Preferably, the enzyme-containing preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase.

[0015] More preferably, the mass ratio of Bacillus licheniformis, alkaline protease, and glucose oxidase in the enzyme-containing preparation is 5 - 7:2 - 4:5 - 7.

[0016] Preferably, the sieving is through a 40 - 60 mesh sieve.

[0017] The lipoic acid added in the present invention has antioxidant effects. Microcrystalline cellulose can improve the texture and stability of mycotoxin degrading agents. L-ascorbic acid, as a potent antioxidant, reduces oxidative stress by scavenging free radicals and protects agricultural products from damage.

[0018] The present invention adds an enzyme-containing preparation made of Bacillus licheniformis, alkaline protease, and glucose oxidase, which has certain advantages in the degradation of vomitoxin; by competing with pathogenic microorganisms for nutrients and living space, it inhibits the growth and reproduction of the latter, reduces the production of vomitoxin, and the extracellular metabolites produced by the bacterial metabolism play a role in biodegradation, rather than the physical adsorption required by aluminosilicates such as montmorillonite for degradation, avoiding the problem of vomitoxin contamination in grains and feeds during actual production; it enhances the protection and stability of agricultural products, reduces the stress response on piglets, promotes feed nutrient absorption, and improves animal growth efficiency.

[0019] Quinoa peptides in the mycotoxin degrading agent of the present invention help to alleviate the physiological discomfort and inflammatory reactions caused by vomitoxin poisoning in animals, can adsorb mycotoxins, separate them from agricultural products, play a role in purifying and removing vomitoxin, enhance the degradation effect of the degrading agent on mycotoxins, and can synergistically enhance the overall performance of the degrading agent with the enzyme-containing preparation.

[0020] The beneficial effects of the present invention:

[0021] 1. Compared with the prior art, the present invention utilizes the interaction between various substances through reasonable proportioning and optimizes the preparation process to obtain a mycotoxin degrading agent. The mycotoxin degrading agent obtained by the present invention has the function of degrading mycotoxins, and particularly has a significant effect on reducing the incidence of diarrhea caused by the stress response of vomitoxin in piglets. It can also promote the absorption of feed nutrients and improve the growth efficiency of animals.

[0022] 2. Compared with the prior art, the lipoic acid of the present invention reacts with chitosan, chitosan oligosaccharide, and polylysine, which have antioxidant properties and certain antibacterial properties, to obtain modified lipoic acid that has both antibacterial and antioxidant properties and has good stability in acidic and alkaline environments. It can play an antibacterial role in different digestion scenarios and reduce the impact of vomitoxin on piglets. Detailed implementation mode

[0023] The parameters and sources of some raw materials in the embodiments of the present invention are as follows:

[0024] Bacillus licheniformis: Its Latin name is Bacillus licheniformis, and the strain number is CGMCC1.10314. It is a commercially available product and is sourced from the China General Microbiological Culture Collection Center;

[0025] Alkaline protease: The enzyme activity is 300,000 U / g;

[0026] Glucose oxidase: The enzyme activity is 20,000 U / g;

[0027] Catalase: The enzyme activity is 300,000 U / g;

[0028] Quinoa peptide: Sourced from a powerful supplier, Xi'an Tongze Biotechnology Co., Ltd., with the product number TZ - 6398745;

[0029] 70wt% chitosan: The degree of deacetylation is ≥85%, sourced from Zhejiang Jinke Pharmaceutical Co., Ltd.;

[0030] Chitosan oligosaccharide: The molecular weight is ≤1000 Da, sourced from Zhejiang Jinke Pharmaceutical Co., Ltd.;

[0031] Polylysine: CAS number: 25104 - 18 - 1, product number: P43050, sourced from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0032] Example 1

[0033] A preparation method of a mycotoxin degrading agent is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of α - lipoic acid, 15 g of enzyme preparation, and 30 g of L - ascorbic acid and passing through a 50 - mesh sieve;

[0034] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0035] Example 2

[0036] A preparation method of a mycotoxin degrader is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of modified α-lipoic acid, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve.

[0037] The preparation method of the modified α-lipoic acid includes the following steps:

[0038] Mix 5 g of chitosan oligosaccharide with 100 g of water evenly, then add 2 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to obtain mixture 1; mix 2 g of α-lipoic acid with 15 g of absolute ethanol evenly, add it to mixture 1, react at 40 °C and 1000 rpm for 24 h, cool to room temperature, add 200 g of 70 wt% ethanol aqueous solution, stir at 1000 rpm for 10 min, let stand, centrifuge at 2000 rpm for 5 min, take the precipitate, add 20 g of water and then freeze-dry for 24 h to obtain the modified α-lipoic acid.

[0039] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0040] Example 3

[0041] A preparation method of a mycotoxin degrader is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of modified α-lipoic acid, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve.

[0042] The preparation method of the modified α-lipoic acid includes the following steps:

[0043] Mix 5 g of chitosan with 100 g of 1.0 wt% acetic acid aqueous solution evenly, then add 2 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to obtain mixture 1; mix 2 g of α-lipoic acid with 15 g of absolute ethanol evenly, add it to mixture 1, react at 40 °C and 1000 rpm for 24 h, cool to room temperature, add 200 g of 70 wt% ethanol aqueous solution, stir at 1000 rpm for 10 min, let stand, centrifuge at 2000 rpm for 5 min, take the precipitate, add 20 g of water and then freeze-dry for 24 h to obtain the modified α-lipoic acid.

[0044] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0045] Example 4

[0046] A preparation method of a mycotoxin degrading agent is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of modified α-lipoic acid, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve;

[0047] The preparation method of the modified α-lipoic acid includes the following steps:

[0048] Mix 5 g of polylysine with 100 g of water evenly, then add 2 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.5 g of N-hydroxysuccinimide to obtain mixture 1; mix 2 g of α-lipoic acid with 15 g of absolute ethanol evenly, add it to mixture 1, react at 40 °C and 1000 rpm for 24 h, cool to room temperature, add 200 g of 70 wt% ethanol aqueous solution, stir at 1000 rpm for 10 min, let stand, centrifuge at 2000 rpm for 5 min, take the precipitate, add 20 g of water and then freeze-dry for 24 h to obtain the modified α-lipoic acid.

[0049] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0050] Comparative Example 1

[0051] A preparation method of a mycotoxin degrading agent is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve;

[0052] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0053] Comparative Example 2

[0054] A preparation method of a mycotoxin degrading agent is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of chitosan oligosaccharide, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve;

[0055] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0056] Comparative Example 3

[0057] A preparation method of a mycotoxin degrading agent is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of chitosan, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve;

[0058] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0059] Comparative Example 4

[0060] A preparation method of a mycotoxin degrading agent is obtained by mixing 15 g of quinoa peptide, 35 g of microcrystalline cellulose, 20 g of polylysine, 15 g of enzyme preparation, and 30 g of L-ascorbic acid and passing through a 50-mesh sieve;

[0061] The enzyme preparation is composed of Bacillus licheniformis, alkaline protease, and glucose oxidase in a mass ratio of 6:3:6.

[0062] Test Example 1

[0063] Diarrhea rate test

[0064] In this experiment, 28-day-old weaned Landrace piglets were selected, and the experimental period was 21 days. The Landrace piglets were randomly divided into 9 groups, with 10 pigs in each group. The experimental groups of Examples 1-4, Comparative Examples 1-4, and the control group were set up for the experiment; among them, the control group was fed with the basal diet group, and the experimental groups of Examples 1-4 and Comparative Examples 1-4 were fed with the basal diet supplemented with 500 g / ton of the mycotoxin degrading agent obtained from each experimental group. During the test period, the piglets were raised in a fully enclosed nursery pig house, and the temperature was controlled at 25-27 °C, with free access to food and water. The basal diet did not contain any antibiotics, and the piglets were immunized according to the conventional immunization program.

[0065] Determination indexes: The production performance of weaned piglets in each treatment group, specifically including the following indexes:

[0066] Observe and record the fecal status of the piglets at 10:00 every morning during the test period, and calculate the diarrhea rate of weaned piglets. The calculation formula is as follows:

[0067] Diarrhea rate (%) = (cumulative number of days of diarrhea of all piglets during the test period) / (total number of pigs × test days) × 100%.

[0068] The test results are shown in Table 1 below:

[0069] Table 1 Diarrhea rate

[0070] Number Diarrhea rate (%) Control group 11.90 Example 1 10.0 Example 2 6.67 Example 3 7.14 Example 4 2.86 Comparative example 1 10.95 Comparative example 2 9.52 Comparative example 3 9.52 Comparative example 4 9.05

[0071] By comparing Examples 1-4, Comparative Examples 1-4 and the control group, it can be found that the diarrhea rates of Comparative Examples 1-4 are lower than those of the control group. By comparing Examples 1-4, it can be found that the diarrhea rate of Example 4 is the lowest, only 2.86%. The reason may be that the α-lipoic acid added in Example 4 is modified by polylysine. Polylysine has a high amino density and more binding sites with the carboxyl groups of α-lipoic acid, and the reaction is more complete. Through amidation reaction, stable covalent bonds are formed, and the structure of the modified product is more stable. The special structure formed may kill Fusarium fungi to prevent the production of new toxins. Polylysine-modified α-lipoic acid may have specific adsorption properties or degradation effects on DON, can enhance the intestinal physical barrier, reduce the penetration of DON and its absorption by the intestine, scavenge free radicals and reduce the production of pro-inflammatory factors. There may be a synergistic effect among the modified lipoic acid, enzyme preparation and quinoa peptide to further reduce the diarrhea rate. In Examples 2 and 3, α-lipoic acid is modified by chitosan oligosaccharide and chitosan. The amino densities of chitosan and chitosan oligosaccharide are lower than that of polylysine, the reaction degree and the content of active functional groups are lower than those of polylysine, and the adsorption effect or degradation effect on DON is lower than that of Example 4. However, the chitosan oligosaccharide in Example 2 has a short-chain structure, small steric hindrance and a higher reaction degree than chitosan, and is more likely to bind to the intestinal mucosa to enhance the physical barrier; the chitosan with a long-chain structure in Example 3 has a larger steric hindrance and a lower reaction degree. Although Comparative Examples 2-4 added natural cationic polymers which have antibacterial properties, chitosan oligosaccharide and chitosan bind to the bacterial cell membrane through positive charges to destroy its integrity, and polylysine interferes with microbial metabolism, and may be able to kill Fusarium fungi to prevent the production of new toxins, but their effects on DON production and scavenging free radicals and reducing the production of pro-inflammatory factors are lower than those of Examples 2-4.

[0072] Test Example 2

[0073] Body weight change test

[0074] Healthy 28-day-old weaned Landrace piglets were selected and randomly divided into 10 groups, with 20 pigs in each group. The groups of Examples 1-4, Comparative Examples 1-4, the control group and the blank group were set up for experiments, and the pigs were fed for 45 days; among them, the control group was fed with feed containing 0.5 mg / kg vomitoxin; Examples 1-4 and Comparative Examples 1-4 were respectively fed with feed containing 0.5 mg / kg vomitoxin added with the mycotoxin degrading agents prepared in Examples 1-4 and Comparative Examples 1-4, and the addition ratio was 500 grams of mycotoxin degrading agent per ton of feed containing 0.5 mg / kg vomitoxin; in addition, the blank group was fed with normal feed without adding vomitoxin and mycotoxin detoxifying agent. During the test period, the piglets were raised in a fully enclosed nursery pig house, the temperature was controlled at 25-27 °C, and they had free access to food and water. Weighing records were taken before and after the experiment, and the data were averaged. The test results are shown in Table 2 below:

[0075] Table 2 Body weight change results

[0076]

[0077]

[0078] The average daily weight gain of piglets fed with feed containing 0.5 mg / kg of vomitoxin in the control group was 190 g; the feed used in the blank group did not add vomitoxin and mycotoxin detoxifier, and the average daily weight gain was 371 g, indicating that DON significantly affected the weight gain of piglets. By comparing Example 1-4, Comparative Example 1-4, the blank group and the control group, it was found that the daily weight gain of Example 4 was slightly higher than that of the blank group. The reason may be that Example 4 added polylysine-modified α-lipoic acid, and the special structure formed may kill Fusarium fungi to prevent the production of new toxins. Polylysine-modified α-lipoic acid may have specific adsorption properties or degradation effects on DON, can enhance the intestinal physical barrier, reduce the penetration of DON and its absorption by the intestine, scavenge free radicals, reduce the production of pro-inflammatory factors, and reduce the impact of DON on piglets. Synergistic with enzyme preparations and quinoa peptides, it can promote nutrient absorption to a certain extent and increase the daily weight gain of piglets.

Claims

1. A preparation method of a mycotoxin degrading agent, characterized in that, By mass parts: obtained by mixing 10 - 20 parts of quinoa peptide, 30 - 40 parts of microcrystalline cellulose, 10 - 30 parts of α-lipoic acid, 10 - 20 parts of enzyme-containing preparation, and 20 - 40 parts of L-ascorbic acid and sieving; Or obtained by mixing 10 - 20 parts of quinoa peptide, 30 - 40 parts of microcrystalline cellulose, 10 - 30 parts of modified α-lipoic acid, 10 - 20 parts of enzyme-containing preparation, and 20 - 40 parts of L-ascorbic acid and sieving; Among them, the modified α-lipoic acid is α-lipoic acid modified by a natural cationic polymer.

2. The preparation method of the mycotoxin degrading agent according to claim 1, characterized in that: The preparation method of the modified α-lipoic acid comprises the following steps, by mass parts: Mix 1 - 10 parts of natural cationic polymer with 80 - 120 parts of solvent evenly, then add 1 - 3 parts of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.1 - 1 part of N-hydroxysuccinimide to obtain mixture 1; Mix 1 - 5 parts of α-lipoic acid with 10 - 20 parts of absolute ethanol evenly, add it to mixture 1, react at 30 - 50 °C and 800 - 1200 rpm for 20 - 28 h; Cool to room temperature, add 150 - 250 parts of 70 wt% ethanol aqueous solution, stir at 800 - 1200 rpm for 5 - 15 min, let stand, centrifuge at 1000 - 3000 rpm for 3 - 8 min, take the precipitate, add 10 - 25 parts of water and then freeze-dry for 20 - 28 h to obtain the modified α-lipoic acid.

3. The preparation method of the mycotoxin degrading agent according to claim 1 or 2, characterized in that: The natural cationic polymer is selected from at least one of chitosan, chitosan oligosaccharide, and polylysine.

4. The preparation method of the mycotoxin degrading agent according to claim 2, characterized in that: The solvent is water or 0.5 - 2.0 wt% acetic acid aqueous solution.

5. The preparation method of the mycotoxin degrading agent according to claim 1, characterized in that: The enzyme-containing preparation consists of Bacillus licheniformis, alkaline protease, and glucose oxidase.

6. The preparation method of the mycotoxin degrading agent according to claim 5, characterized in that: The mass ratio of Bacillus licheniformis, alkaline protease, and glucose oxidase in the enzyme-containing preparation is 5 - 7:2 - 4:5 - 7.

7. The preparation method of the mycotoxin degrading agent according to claim 1, wherein: The sieving is through a 40 - 60 mesh sieve.

8. A mycotoxin degrader, characterized in that: Prepared by the method according to any one of claims 1 - 7.

9. Use of the mycotoxin degrading agent according to claim 8, characterized in that: The mycotoxin degrader is applied to a drug for relieving stress response in piglets.

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