Full-automatic nucleic acid treatment system

Abstract

The invention discloses a full-automatic nucleic acid treatment system which comprises a control assembly, a detection box carrier assembly, an extraction and purification pipetting assembly, a temperature control assembly, a driving assembly and a fluorescence channel assembly, and the control assembly controls the detection box carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the driving assembly and the fluorescence channel assembly; the extraction and purification pipetting assembly is arranged above the detection box carrier assembly in a liftable manner and is used for pipetting, oscillation and / or magnetic attraction in the nucleic acid extraction process; the driving assembly is used for driving the detection box carrier assembly to move back and forth along a first direction and driving the extraction and purification pipetting assembly to ascend and descend along a second direction; the temperature control assembly is arranged below the fixing sleeve and is used for providing a temperature condition for the nucleic acid extraction detection box in the fixing sleeve; and the fluorescent channel assembly is used for performing PCR detection on a sample in the nucleic acid extraction detection box.

Description

Technical Field

[0001] This application relates to the field of molecular diagnostic technologies, and particularly to a fully automated nucleic acid processing system. Background Art

[0002] In the field of molecular biology research, nucleic acid extraction and PCR amplification detection are two core experimental techniques. With the continuous progress of technology, the field of experimental science is undergoing a transformation from traditional manual operations to automated equipment. Automated equipment, with its excellent repeatability and consistency, significantly reduces the cumulative errors caused by manual operations, greatly improving the efficiency of experiments and the accuracy of results.

[0003] DNA methylation is an important research area in epigenetics, and changes in its status are considered to be one of the key factors in tumorigenesis. Such changes include a decrease in the overall genomic methylation level and an abnormal increase in the local methylation level of CpG islands. These changes may lead to genomic instability and thus induce cell carcinogenesis. Compared with gene mutations, epigenetic variations are more frequent. Therefore, using these variations for tumor diagnosis can provide higher sensitivity and specificity. As an early event in tumorigenesis, DNA methylation has important clinical application value for early tumor diagnosis by detecting the methylation level of genes.

[0004] Circulating tumor DNA (ctDNA) is a DNA fragment from the tumor genome present in the human blood system. The main sources are necrotic tumor cells, apoptotic tumor cells, circulating tumor cells, and exosomes secreted by tumor cells. These tumor DNAs often contain methylation characteristics unique to the tumor genome.

[0005] Currently, the purification, bisulfite treatment, and nucleic acid detection of free DNA in plasma mostly adopt a method of manual linkage of multiple platforms. This not only requires the use of multiple devices in combination, but also requires multiple manual interventions during the process, resulting in inconsistent sample stagnation times, extended detection times, poor sample repeatability, and increased cumulative errors between multiple platforms. The difficulties of automated equipment are as follows: Difficulty 1: Realization of full-process detection: How to achieve full-process detection from sample processing to result output on one device to eliminate or reduce the cumulative errors between multiple platforms and multiple devices and reduce manual intervention. Difficulty 2: Closed nucleic acid detection: How to complete closed nucleic acid detection inside the device or in supporting consumables to meet the requirements of a dedicated PCR detection environment. Difficulty 3: Large-volume sample processing: Currently, nucleic acid detection mainly targets small-volume samples and lacks the ability to process large-volume samples (such as 10 ml of processing mixture). Summary of the Invention

[0006] Based on the above existing defect problems, the present application provides a fully automatic nucleic acid methylation processing system. The entire process from the plasma sample entering the device to obtaining the PCR test result of the target DNA is completely completed by the device without manual intervention, and highly automated nucleic acid methylation extraction and detection can be achieved.

[0007] The present application provides the following technical solutions.

[0008] 1. A fully automatic nucleic acid processing system, which includes a control component, a detection cassette carrier component, an extraction and purification pipetting component, a temperature control component, a driving component, and a fluorescence channel component. Among them,

[0009] The control component controls the detection cassette carrier component, the extraction and purification pipetting component, the temperature control component, the driving component, and the fluorescence channel component respectively;

[0010] The detection cassette carrier component is used to place the nucleic acid extraction and detection cassette;

[0011] The detection cassette carrier component includes a first fixing part and at least one set of heating fixing parts. The first fixing part includes a first fixing member and a second fixing member arranged oppositely. Between the first fixing member and the second fixing member, at least one set of the heating fixing parts is provided. Each set of the heating fixing parts includes at least two fixing sleeves arranged at intervals. The first fixing member and the second fixing member are respectively used to limit the two ends of the nucleic acid extraction and detection cassette, and the fixing sleeve is used to limit a part of the nucleic acid extraction and detection cassette;

[0012] The extraction and purification pipetting component is arranged above the detection cassette carrier component in a liftable manner, and is used for pipetting, oscillating, and / or magnetic absorption during the nucleic acid extraction process;

[0013] The driving component is used to drive the detection cassette carrier component to move back and forth in a first direction, and drive the extraction and purification pipetting component to lift in a second direction;

[0014] The temperature control component is arranged below the fixing sleeve, and is used to provide temperature conditions for the nucleic acid extraction and detection cassette in the fixing sleeve;

[0015] The fluorescence channel component is used to perform PCR detection on the sample in the nucleic acid extraction and detection cassette.

[0016] 2. The system according to item 1, wherein the side wall of the first fixing member facing the second fixing member has at least one set of first fixing grooves recessed into its interior,

[0017] The side wall of the second fixing member facing the first fixing member has at least one set of second fixing grooves recessed into its interior.

[0018] 3. The system according to item 2, wherein a detection hole position is further provided between the first fixing member and the second fixing member, on a side of the detection hole position close to the first fixing member, and the number of the heating fixing parts, the number of the detection hole positions are equal to the number of the first fixing grooves and the second fixing grooves.

[0019] 4. The system according to item 3, wherein the system further comprises a first bottom plate, the driving assembly comprises a first driving assembly, the temperature control assembly is arranged on the first bottom plate, and the first driving assembly is used for driving the first bottom plate to move back and forth along a first direction, so as to drive the temperature control assembly and the detection cartridge carrier assembly to move back and forth along the first direction.

[0020] 5. The system according to item 4, wherein the system further comprises a second bottom plate, the first driving assembly is arranged on the second bottom plate, and the first bottom plate moves on the second bottom plate along the first direction.

[0021] 6. The system according to item 1, wherein the extraction and purification pipetting assembly comprises a magnetic rod rack assembly and a multi-channel pipetting assembly, the magnetic rod rack assembly is arranged above the detection cartridge carrier assembly in a liftable manner and is used for oscillating and / or magnetic absorption during nucleic acid extraction, and the multi-channel pipetting assembly is arranged above the detection cartridge carrier assembly in a liftable manner and is used for driving a syringe to perform pipetting during nucleic acid extraction.

[0022] 7. The system according to item 6, wherein the system further comprises a fourth bottom plate, a first bracket, a second bracket, a fourth bracket and a fifth bracket, the fourth bottom plate is fixedly connected with the second bottom plate through a support column, and the first bracket, the second bracket, the fourth bracket and the fifth bracket are all connected with the fourth bottom plate.

[0023] 8. The system according to item 7, wherein the magnetic rod rack assembly comprises a plurality of magnetic rods and magnetic rod sleeves arranged in an array, the magnetic rods are located above the magnetic rod sleeves and can move downward into the magnetic rod sleeves.

[0024] 9. The system according to item 8, wherein the driving assembly further comprises a second driving assembly and a third driving assembly, the second driving assembly is used for driving the magnetic rods to move up and down along a second direction, and the third driving assembly is used for driving the magnetic rod sleeves to move up and down along the second direction;

[0025] The magnetic rod rack assembly is located above the nucleic acid extraction detection cartridge, and the second driving assembly and the third driving assembly are used for driving the magnetic rod rack assembly or the magnetic rod sleeves to move above the nucleic acid extraction detection cartridge and can move downward to enable the magnetic rod rack assembly or the magnetic rod sleeves to extend into the nucleic acid extraction detection cartridge.

[0026] 10. The system according to item 8 or 9, wherein m columns of magnetic bars are arranged on the first bracket, the distance between the m columns of magnetic bars is adjustable, each column has n magnetic bars, and the distance between the n magnetic bars is adjustable;

[0027] m columns of magnetic bar sleeves are arranged on the second bracket, the distance between the m columns of magnetic bar sleeves is adjustable, each column has n magnetic bar sleeves, and the distance between the n magnetic bar sleeves is adjustable;

[0028] where m≥1 and n≥2.

[0029] 11. The system according to item 7, wherein the multi-channel pipetting assembly includes a syringe barrel driving part and a piston driving part, a part of the piston driving part is sleeved in the syringe barrel driving part, and the piston driving part is arranged in the syringe barrel driving part so as to be liftable along a second direction;

[0030] The syringe barrel driving part is used to drive the syringe barrel of the syringe to move up and down along the second direction;

[0031] The piston driving part is used to drive the piston of the syringe to move up and down along the second direction.

[0032] 12. The system according to item 11, wherein the driving assembly further includes a fourth driving assembly and a fifth driving assembly, the fourth driving assembly is used to drive the syringe barrel driving part to move up and down along the second direction, and the fifth driving assembly is used to drive the piston driving part to move up and down along the second direction;

[0033] The multi-channel pipetting assembly is located above the nucleic acid extraction and detection kit, and drives the multi-channel pipetting assembly or the syringe barrel driving part to move above the nucleic acid extraction and detection kit through the fourth driving assembly and the fifth driving assembly, and can move downward so that the syringe barrel extends into the nucleic acid extraction and detection kit.

[0034] 13. The system according to item 11 or 12, wherein m columns of syringe barrel driving parts are arranged on the fourth bracket, the distance between the m columns of syringe barrel driving parts is adjustable, each column has n syringe barrel driving parts, and the distance between the n syringe barrel driving parts is adjustable, or

[0035] a syringe barrel driving part assembly is arranged on the fourth bracket, and the syringe barrel driving part assembly has m columns of spaced syringe barrel driving parts, the distance between the m columns of syringe barrel driving parts is adjustable, each column has n syringe barrel driving parts, and the distance between the n syringe barrel driving parts is adjustable;

[0036] The fifth bracket is provided with m columns of piston driving parts, the spacing between the m columns of piston driving parts is adjustable, each column has n piston driving parts, and the spacing between the n piston driving parts is adjustable; where m≥1 and n≥2.

[0037] 14. The system according to item 1, wherein the system further includes a third bottom plate, the third bottom plate is arranged above the detection cartridge carrier assembly, and the middle of the third bottom plate has an opening, and when the multi-channel pipetting assembly and the magnetic rod holder assembly move along the second direction, they penetrate through the opening.

[0038] 15. The system according to item 14, wherein the driving assembly further includes a sixth driving assembly, and the sixth driving assembly is used to drive the third bottom plate to move up and down along the second direction.

[0039] 16. The system according to item 15, wherein a first pressing plate and a second pressing plate are arranged on one side surface of the third bottom plate facing the detection cartridge carrier assembly, the first pressing plate is located above the first fixing member, the second pressing plate is located above the second fixing member, and the sixth driving assembly drives the first pressing plate and the second pressing plate to descend along the second direction, so that the first pressing plate and the second pressing plate press on the top of the nucleic acid extraction detection cartridge, thereby fixing the nucleic acid extraction detection cartridge on the detection cartridge carrier assembly by the first pressing plate and the second pressing plate.

[0040] 17. The system according to item 16, wherein a first card slot is arranged on one side surface of the first pressing plate facing the nucleic acid extraction detection cartridge, a second card slot is arranged on one side surface of the second pressing plate facing the nucleic acid extraction detection cartridge, and the first card slot and the second card slot are used for clamping the nucleic acid extraction detection cartridge.

[0041] 18. The system according to item 4, wherein the fluorescence channel assembly is arranged on the first bottom plate, and the optical fiber of the fluorescence channel assembly extends into the detection hole position, so as to perform PCR detection on the sample in the nucleic acid extraction detection cartridge.

[0042] 19. The system according to item 1, wherein the temperature control component includes a heating unit and a cooling unit, the heating unit is arranged directly below the fixed sleeve, and the cooling unit is arranged on one side of the heating unit and is used for cooling the heating unit.

[0043] 20. The system according to item 1, wherein the system further includes a display mechanism, and the display mechanism is connected to the control mechanism and is used to issue instructions to the control mechanism.

[0044] The fully automatic nucleic acid methylation processing system described in this application fixes the nucleic acid extraction and detection kit through the detection kit carrier assembly, then adds a plasma sample into the nucleic acid extraction and detection kit. The driving assembly drives the multi-channel pipetting assembly in the extraction and purification pipetting assembly to drive a syringe to transfer different reagents to corresponding positions in the nucleic acid extraction and detection kit. The driving assembly drives the magnetic rod rack assembly to oscillate and extract the sample solution in the nucleic acid extraction and detection kit. Since the syringe needs to transfer different reagents to different positions in the nucleic acid extraction and detection kit, the multi-channel pipetting assembly can only move up and down along the second direction, and the driving assembly drives the detection kit carrier assembly to move along the first direction, so that the multi-channel pipetting assembly drives the syringe to transfer different reagents to different positions in the nucleic acid extraction and detection kit. After the plasma sample in the nucleic acid extraction and detection kit is processed, the fluorescence channel assembly performs PCR detection on the sample in the nucleic acid extraction and detection kit, thus completing the extraction and PCR detection of the plasma sample.

[0045] In the fully automatic nucleic acid methylation processing system described in this application, the entire process from the plasma sample entering the device to obtaining the PCR detection result of the target DNA is completely completed by the device without manual intervention, and highly automated nucleic acid methylation extraction and detection can be achieved. Moreover, each mechanism in this application has strong independence. While the mechanisms can cooperate with each other, a single mechanism can also complete the corresponding work; the relative positions of all mechanisms can change according to the limitations of the placement space, and the relative positions are flexible and variable. Description of the Drawings

[0046] Figure 1 It is a schematic structural diagram of the fully automatic nucleic acid methylation processing system provided by this application.

[0047] Figure 2 It is a schematic structural diagram of the fully automatic nucleic acid methylation processing system provided by this application.

[0048] Figure 3 It is a schematic structural diagram of the fully automatic nucleic acid methylation processing system provided by this application.

[0049] Figure 4 It is a schematic structural diagram of the fully automatic nucleic acid methylation processing system provided by this application.

[0050] Figure 5 It is a schematic structural diagram of the fully automatic nucleic acid methylation processing system provided by this application.

[0051] Description of the Reference Numerals

[0052] 1 - First bottom plate, 2 - Second bottom plate, 3 - Third bottom plate, 4 - Fourth bottom plate 4, 5 - First bracket, 6 - Second bracket, 7 - Fourth bracket, 8 - First drive motor, 9 - Fifth bracket, 10 - Third drive motor, 11 - Fourth drive motor, 12 - Fifth drive motor, 13 - Sixth drive motor, 14 - Support column, 15 - First fixing member, 16 - Second fixing member, 17 - First pressing plate, 18 - Heating unit, 19 - Cooling unit, 20 - Second drive motor, 21 - Piston drive part, 22 - Syringe drive part assembly, 23 - Syringe, 24 - Magnetic rod holder assembly, 25 - Fixed sleeve. Detailed implementation manner

[0053] The following describes exemplary embodiments of the present application, including various details of the embodiments of the present application to facilitate understanding, which should be considered merely exemplary. Therefore, those of ordinary skill in the art should recognize that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of the present application. Similarly, for the sake of clarity and conciseness, descriptions of well-known functions and structures are omitted in the following description.

[0054] As Figures 1 - 5 shown, the present application provides a full-automatic nucleic acid processing system, which includes a control component, a test cartridge carrier assembly, an extraction and purification pipetting assembly, a temperature control component, a drive component, and a fluorescence channel component. Among them,

[0055] The control component controls the test cartridge carrier assembly, the extraction and purification pipetting assembly, the temperature control component, the drive component, and the fluorescence channel component respectively;

[0056] The test cartridge carrier assembly is used to place a nucleic acid extraction test cartridge; herein, the nucleic acid extraction test cartridge can be a conventional multi-well deep well plate (such as a 96-well plate or a 48-well plate, etc.), or a multi-well deep well plate with a liquid storage function. The multi-well deep well plate with a liquid storage function means that in the multi-well deep well plate, there are liquid storage holes and experimental holes. Reagents and magnetic beads required in the nucleic acid extraction and purification process are stored in the liquid storage holes, and the experimental holes are used to provide space required for test steps such as nucleic acid extraction and purification.

[0057] Multiple nucleic acid extraction test cartridges can be placed in the test cartridge carrier assembly, so that multiple samples can be extracted simultaneously.

[0058] The detection cassette carrier assembly includes a first fixing part and at least one set of heating fixing parts. The first fixing part includes a relatively arranged first fixing member 15 and a second fixing member 16. Between the first fixing member 15 and the second fixing member 16, there is at least one set of the heating fixing parts. Each set of the heating fixing parts includes at least two fixing sleeves 25 arranged at intervals. The first fixing member 15 and the second fixing member 16 are respectively used to limit the two ends of the nucleic acid extraction detection cassette, and the fixing sleeve 25 is used to limit a partial area of the nucleic acid extraction detection cassette; when the nucleic acid extraction detection cassette has multiple holes, some of the holes can be placed inside the fixing sleeve 25.

[0059] The heights of the first fixing member 15 and the second fixing member 16 are higher than the height of the fixing sleeve 25.

[0060] The number of the fixing sleeves 25 in each set of the heating fixing parts can be multiple, such as 2, 3, 4, 5, 6, etc., and the number of the fixing sleeves 25 can be determined according to actual needs. The shapes and sizes of the fixing sleeves 25 in each set of the heating fixing parts can be the same or different. For example, the shape of the fixing sleeve 25 can be a columnar structure or a strip-shaped card slot structure, etc.

[0061] The number of the heating fixing parts can be 1 group, 2 groups, 3 groups, 4 groups, 5 groups, 6 groups, 7 groups, 8 groups, 9 groups, 10 groups, etc., and the number of the heating fixing parts can be determined according to actual needs.

[0062] The extraction and purification pipetting assembly is disposed above the detection cassette carrier assembly in a liftable manner, and is used for pipetting, oscillating and / or magnetic attraction during the nucleic acid extraction process.

[0063] Further, the extraction and purification pipetting assembly includes a magnetic rod holder assembly 24 and a multi-channel pipetting assembly. The magnetic rod holder assembly 24 is disposed above the detection cassette carrier assembly in a liftable manner, and is used for oscillating and / or magnetic attraction during the nucleic acid extraction process. The multi-channel pipetting assembly is disposed above the detection cassette carrier assembly in a liftable manner, and is used to drive a syringe to perform pipetting during the nucleic acid extraction process;

[0064] The driving assembly is used to drive the detection cassette carrier assembly to perform a reciprocating motion in a first direction, and drive the extraction and purification pipetting assembly to lift in a second direction.

[0065] Further, the driving assembly is used to drive the detection cassette carrier assembly to perform a reciprocating motion in a first direction, drive the magnetic rod holder assembly 24 to lift in a second direction, and drive the multi-channel pipetting assembly to lift in a second direction;

[0066] In this article, the first direction is perpendicular to the second direction.

[0067] The temperature control component is arranged below the fixing sleeve 25 and is used to provide temperature conditions for the nucleic acid extraction and detection cartridge in the fixing sleeve 25;

[0068] The fluorescence channel component is used to perform PCR detection on the sample in the nucleic acid extraction and detection cartridge.

[0069] In the full-automatic nucleic acid methylation processing system of the present application, the nucleic acid extraction and detection cartridge is fixed by the detection cartridge carrier assembly, and then plasma is added into the nucleic acid extraction and detection cartridge. The multi-channel pipetting assembly in the extraction and purification pipetting assembly drives a syringe to transfer different reagents to corresponding positions in the nucleic acid extraction and detection cartridge through the driving assembly. The magnetic rod holder assembly 24 drives the sample solution in the nucleic acid extraction and detection cartridge to oscillate and extract through the driving assembly. Since the syringe needs to transfer different reagents to different positions in the nucleic acid extraction and detection cartridge, the multi-channel pipetting assembly can only move up and down along the second direction. The driving assembly drives the detection cartridge carrier assembly to move along the first direction, so that the multi-channel pipetting assembly drives the syringe to transfer different reagents to different positions in the nucleic acid extraction and detection cartridge. After the plasma sample in the nucleic acid extraction and detection cartridge is processed, the fluorescence channel component performs PCR detection on the sample in the nucleic acid extraction and detection cartridge, thus completing the extraction and PCR detection of the plasma sample.

[0070] In the present application, the side wall of the first fixing member 15 facing the second fixing member 16 has at least one set of first fixing grooves recessed inward, and the side wall of the second fixing member 16 facing the first fixing member 15 has at least one set of second fixing grooves recessed inward.

[0071] Both ends of the nucleic acid extraction and detection cartridge can be respectively clamped on the first fixing groove and the second fixing groove, so as to limit the position of the nucleic acid extraction and detection cartridge, ensure that the nucleic acid extraction and detection cartridge will not move during the nucleic acid extraction process, and avoid affecting the nucleic acid extraction process.

[0072] In some embodiments, when the nucleic acid extraction and detection cartridge has a row of hole positions, the nucleic acid extraction and detection cartridge is arranged along the direction from the first fixing member 15 to the second fixing member 16. With this design, the detection cartridge carrier assembly of the present application can fix multiple nucleic acid extraction and detection cartridges at the same time, so that nucleic acid extraction and purification of multiple samples can be performed simultaneously.

[0073] Furthermore, a detection hole position is also arranged between the first fixing member 15 and the second fixing member 16, on the side of the detection hole position close to the first fixing member 15. The number of the heating and fixing parts, the number of the detection hole positions are equal to the number of the first fixing grooves and the second fixing grooves.

[0074] Further, in the direction from the first fixing member 15 to the second fixing member 16, a detection hole positions and a set of heating and fixing parts are sequentially arranged between the first fixing member 15 and the second fixing member 16. Each set of heating and fixing parts includes b fixing sleeves 25 arranged at intervals. Each detection hole position corresponds to a set of heating and fixing parts, and the detection hole position and the corresponding plurality of fixing sleeves 25 are on the same straight line. Wherein a is greater than or equal to 1, and b is greater than or equal to 2.

[0075] In some embodiments, in the direction from the first fixing member 15 to the second fixing member 16, a detection hole position and a set of heating and fixing parts are sequentially arranged between the first fixing member 15 and the second fixing member 16. Each set of heating and fixing parts includes 4 fixing sleeves 25 arranged at intervals.

[0076] In some embodiments, in the direction from the first fixing member 15 to the second fixing member 16, two detection hole positions and two sets of heating and fixing parts are sequentially arranged between the first fixing member 15 and the second fixing member 16. Each set of heating and fixing parts includes 4 fixing sleeves 25 arranged at intervals.

[0077] In the present application, the system further includes a first bottom plate 1. The driving assembly includes a first driving assembly. The temperature control assembly is arranged on the first bottom plate 1. The first driving assembly is used to drive the first bottom plate 1 to move back and forth in the first direction, thereby driving the temperature control assembly and the detection cartridge carrier assembly on the temperature control assembly to move back and forth in the first direction. When it is necessary to pipette or oscillate at different positions in the nucleic acid extraction and detection cartridge, the first driving assembly drives the detection cartridge carrier assembly and the nucleic acid extraction and detection cartridge thereon to move in the first direction.

[0078] The first direction is consistent with the direction from the first fixing member 15 to the second fixing member 16.

[0079] Further, the system further includes a second bottom plate 2. The first driving assembly is arranged on the second bottom plate 2. The first bottom plate 1 moves on the second bottom plate 2 in the first direction.

[0080] The first driving assembly includes a first driving motor 8, a first driving shaft, a first slider and a first slide rail. The first slide rail is arranged on the second bottom plate 2. The first slider is arranged at the bottom of the first bottom plate 1. The first driving motor 8 is connected to the first driving shaft, and the first driving shaft is connected to the slider. The first driving motor 8 can drive the first driving shaft to move back and forth in the first direction, thereby driving the first slider to move back and forth in the first chute in the first direction, and further driving the first bottom plate 1 and each part structure on the first bottom plate 1 to move back and forth in the first direction.

[0081] In the present application, the system further includes a fourth base plate 4, a first bracket 5, a second bracket 6, a fifth bracket 9, and a fourth bracket 7. The fourth base plate 4 is fixedly connected to the second base plate 2 through support columns 14. The first bracket 5, the second bracket 6, the fifth bracket 9, and the fourth bracket 7 are all connected to the fourth base plate 4. The second base plate is placed on a tabletop or the ground, and the fourth base plate is fixed to the second base plate through the support columns. The first bracket, the second bracket, the fourth bracket, and the fifth bracket are directly or indirectly connected to the fourth base plate. With such a design, while driving the magnetic bar, the magnetic bar sleeve, and the pipetting assembly to move up and down, the compactness of the structure can be ensured, making the device smaller in volume and more flexible in transmission.

[0082] The number of the support columns 14 can be 4, and the 4 support columns 14 support the fourth base plate 4 above the second base plate 2.

[0083] Further, the magnetic bar rack assembly 24 includes a plurality of magnetic bars and magnetic bar sleeves arranged in an array. The magnetic bars are located above the magnetic bar sleeves and can move downward and extend into the magnetic bar sleeves.

[0084] Further, the driving assembly further includes a second driving assembly and a third driving assembly. The second driving assembly is used to drive the magnetic bar to move up and down in a second direction, and the third driving assembly is used to drive the magnetic bar sleeve to move up and down in the second direction;

[0085] The magnetic bar rack assembly 24 is located above the nucleic acid extraction and detection box. The second driving assembly and the third driving assembly drive the magnetic bar rack assembly 24 or the magnetic bar sleeve to move above the nucleic acid extraction and detection box and can move downward to make the magnetic bar rack assembly 24 or the magnetic bar sleeve extend into the nucleic acid extraction and detection box.

[0086] The second driving assembly includes a second driving motor 20 and a second driving shaft. The second driving motor 20 is connected to the second driving shaft, and the second driving shaft is connected to the first bracket 5. Thus, when the second driving motor 20 drives the second driving shaft to move up and down in the second direction, the first bracket 5 also moves up and down in the second direction, and further drives the magnetic bar to move up and down in the second direction.

[0087] The third driving assembly includes a third driving motor 10 and a third driving shaft. The third driving motor 10 is connected to the third driving shaft, and the third driving shaft is connected to the second bracket 6. Therefore, when the third driving motor 10 drives the third driving shaft to move up and down along the second direction, the second bracket 6 will also move up and down along the second direction, thereby driving the magnetic rod sleeve to move up and down along the second direction.

[0088] Further, the first bracket 5 includes a first fixed bracket and a first connecting bracket, the first fixed bracket is connected to the second driving shaft, and the first connecting bracket is connected to the magnetic rod. The second bracket 6 includes a second fixed bracket and a second connecting bracket, the second fixed bracket is connected to the third driving shaft, the second connecting bracket is connected to the magnetic rod sleeve, the first connecting bracket has a first through hole, and the second connecting bracket penetrates the first through hole so that the first connecting bracket is located above the second connecting bracket, thereby making the magnetic rod located directly above the magnetic rod sleeve.

[0089] Furthermore, a first limiting cylinder surrounding the first through hole is provided on the first connecting bracket, and the second connecting bracket passes through the first limiting cylinder and the first through hole. When the second connecting bracket is lifted or lowered, the first connecting bracket is not affected, and the first limiting cylinder can limit the displacement or shaking of the second connecting bracket when it is lifted or lowered.

[0090] Furthermore, the first bracket 5 is provided with m columns of magnetic bars, the spacing between the m columns of magnetic bars is adjustable, each column has n magnetic bars, and the spacing between the n magnetic bars is adjustable;

[0091] The second bracket 6 is provided with m rows of magnetic rod sleeves, the spacing between the m rows of magnetic rod sleeves is adjustable, each row has n magnetic rod sleeves, and the spacing between the n magnetic rod sleeves is adjustable;

[0092] Where m≥1, n≥2.

[0093] m can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc., n can be 2, 3, 4, 5, 6, 7, 8, 9, 10, etc. The number of m and n can be designed according to actual needs.

[0094] The adjustable spacing between adjacent magnetic bars / magnetic bar sleeves can be achieved by a telescopic rod.

[0095] In some embodiments, the first support 5 is provided with 12 magnetic bars in a row, and the 12 magnetic bars are arranged at equal intervals. The second support 6 is provided with 12 magnetic bar sleeves in a row, and the 12 magnetic bar sleeves are arranged at equal intervals.

[0096] In the present application, the multi-channel pipetting assembly includes a syringe barrel driving part and a piston driving part 21. A partial area of the piston driving part 21 is sleeved inside the syringe barrel driving part, and the piston driving part 21 is arranged to be liftable along a second direction inside the syringe barrel driving part.

[0097] The syringe includes a syringe barrel 23 and a piston. The syringe barrel driving part is connected to the syringe barrel 23, so as to drive the syringe barrel 23 to lift along the second direction through the syringe barrel driving part; the piston driving part 21 is connected to the piston, and drives the piston to lift along the second direction through the piston driving part 21.

[0098] In some embodiments, one end of the piston driving part 21 sleeved inside the syringe barrel driving part is connected to the piston. The piston is sleeved inside the syringe barrel driving part. The syringe barrel 23 is connected to one end of the syringe barrel driving part away from the piston driving part 21. A partial area of the piston extends into the syringe barrel 23. Thus, the lifting of the syringe barrel 23 and the piston is respectively controlled by the syringe barrel driving part and the piston driving part 21 to perform pipetting.

[0099] Furthermore, the driving assembly further includes a fourth driving assembly and a fifth driving assembly. The fourth driving assembly is used to drive the syringe barrel driving part to lift along the second direction, and the fifth driving assembly is used to drive the piston driving part 21 to lift along the second direction;

[0100] The multi-channel pipetting assembly is located above the nucleic acid extraction and detection kit. The fourth driving assembly and the fifth driving assembly drive the multi-channel pipetting assembly or the syringe barrel driving part to move above the nucleic acid extraction and detection kit, and can move downward to make the syringe barrel 23 extend into the nucleic acid extraction and detection kit.

[0101] The fourth driving assembly includes a fourth driving motor 11 and a fourth driving shaft. The fourth driving motor 11 is connected to the fourth driving shaft. The fourth driving motor 11 drives the fourth driving shaft to perform a lifting movement along the second direction. The fourth driving motor 11 is connected to the fourth bottom plate 4, and the fourth driving shaft is connected to the fourth support 7. The fourth driving motor 11 drives the fourth driving shaft to lift along the second direction, and further drives the fourth support 7 and the syringe barrel driving part to lift along the second direction.

[0102] The fifth drive assembly includes a fifth drive motor 12 and a fifth drive shaft, wherein the fifth drive motor 12 is connected to the fifth drive shaft, and the fifth drive motor 12 drives the fifth drive shaft to move up and down along the second direction. The fifth drive motor 12 is connected to the fourth bracket 7, and the fifth drive shaft is connected to the fifth bracket 9. The fifth drive motor 12 drives the fifth drive shaft to move up and down along the second direction, thereby driving the fifth bracket 9 and the piston driving part 21 to move up and down along the second direction.

[0103] The fifth bracket 9 includes a fifth fixed bracket and a fifth limiting sleeve, the two ends of the fifth fixed bracket are provided with fifth through holes, the fifth limiting sleeve is arranged around the fifth through hole, and the fifth fixed bracket is connected with a piston driving part 21 along the second direction. The fourth bracket 7 includes a fourth fixed bracket and a fourth connecting bracket, the fourth fixed bracket is connected to the fourth driving shaft, the fourth fixed bracket and the fourth bottom plate 4 can be lifted and lowered through the fourth limiting slide rail, the fourth connecting bracket penetrates the fifth limiting sleeve and the fifth through hole and is connected to the syringe driving part, the fourth connecting bracket does not affect the fifth fixed bracket when it is lifted and lowered, and the fifth limiting sleeve can limit the displacement or shaking of the fourth connecting bracket when it is lifted and lowered.

[0104] Furthermore, the fifth bracket 9 is provided with m rows of piston driving parts 21, the spacing between the m rows of piston driving parts 21 is adjustable, each row has n piston driving parts 21, the spacing between the n piston driving parts 21 is adjustable; wherein m≥1, n≥2.

[0105] In some embodiments, the fourth bracket 7 is provided with m rows of syringe drive units, the spacing between the m rows of syringe drive units is adjustable, each row has n syringe drive units, the spacing between the n syringe drive units is adjustable; wherein m≥1, n≥2.

[0106] In some embodiments, a syringe drive unit assembly 22 is provided on the fourth bracket 7, and the syringe drive unit assembly 22 has m rows of syringe drive units spaced apart from each other, the spacing between the m rows of syringe drive units is adjustable, each row has n syringe drive units, and the spacing between the n syringe drive units is adjustable; wherein m≥1, n≥2.

[0107] Furthermore, the number of the extraction and purification pipetting components is at least 1 group, for example, the number can be 1 group, 2 groups, 3 groups, 4 groups, 5 groups, 6 groups, 7 groups, 8 groups, 9 groups, 10 groups, etc.

[0108] Further, the number of the magnetic bar holders 24 is at least one group, for example, the number can be 1 group, 2 groups, 3 groups, 4 groups, 5 groups, 6 groups, 7 groups, 8 groups, 9 groups, 10 groups, etc.

[0109] In some embodiments, the number of the fourth brackets 7 and the fifth brackets 9 is two groups. The two groups of fourth brackets 7 are arranged in parallel, and the two groups of fifth brackets 9 are arranged in parallel. The number of the multi-channel pipetting assemblies is two groups, and the number of the magnetic bar holders 24 is one group. The two groups of multi-channel pipetting assemblies are arranged in parallel, and the magnetic bar holder 24 is arranged between the two groups of multi-channel pipetting assemblies. An opening channel is provided on the fourth bottom plate 4, and both of the two groups of multi-channel pipetting assemblies vertically penetrate through the fourth bottom plate 4 through the opening channel.

[0110] In the present application, the system further includes a third bottom plate 3. The third bottom plate 3 is arranged above the detection cassette carrier assembly, and the middle of the third bottom plate 3 has an opening. When the multi-channel pipetting assembly and the magnetic bar holder 24 move along the second direction, they can penetrate through the opening.

[0111] The third bottom plate 3 is arranged between the fourth bottom plate 4 and the first bottom plate 1. The third bottom plate 3 is movably arranged on the support columns 14.

[0112] A sliding sleeve is arranged on the support column 14, and the third bottom plate 3 is sleeved on the sliding sleeve, so that the third bottom plate 3 slides on the support column 14 through the sliding sleeve and then slides.

[0113] Further, the driving assembly further includes a sixth driving assembly, and the sixth driving assembly is used to drive the third bottom plate 3 to move up and down along the second direction.

[0114] The sixth driving assembly includes a sixth driving motor 13 and a sixth driving shaft. The sixth driving motor 13 is connected to the sixth driving shaft, and the sixth driving motor 13 drives the sixth driving shaft to move up and down along the second direction. The sixth driving motor 13 is connected to the fourth bottom plate 4, and the sixth driving shaft is connected to the third bottom plate 3. By driving the sixth driving shaft to move up and down along the second direction by the sixth driving motor 13, the third bottom plate 3 is driven to move up and down along the second direction.

[0115] Further, on one side surface of the third base plate 3 facing the test cartridge carrier assembly, a first pressing plate 17 and a second pressing plate are provided. The first pressing plate 17 is located above the first fixing member 15, and the second pressing plate is located above the second fixing member 16. The sixth driving assembly drives the first pressing plate 17 and the second pressing plate to descend along the second direction, so that the first pressing plate 17 and the second pressing plate press on the top of the nucleic acid extraction test cartridge, thereby pressing the first pressing plate 17 and the second pressing plate against the nucleic acid extraction test cartridge and limiting it on the test cartridge carrier assembly, ensuring that the nucleic acid extraction test cartridge will not move during the nucleic acid extraction process and avoiding affecting the nucleic acid extraction process.

[0116] Further, on one side surface of the first pressing plate 17 facing the nucleic acid extraction test cartridge, a first card slot is provided, and on one side surface of the second pressing plate facing the nucleic acid extraction test cartridge, a second card slot is provided. The first card slot and the second card slot are used for clamping the nucleic acid extraction test cartridge.

[0117] The number of the first card slots on the first pressing plate 17 is the same as the number of the second card slots on the second pressing plate, and each first card slot corresponds to a second card slot. Specifically, the number of the first card slots and the second card slots can both be greater than or equal to 1. In some embodiments, the number of the first card slots and the second card slots can both be greater than or equal to 5, the number of the first card slots and the second card slots can both be greater than or equal to 10, and the number of the first card slots and the second card slots can both be greater than or equal to 15. Specifically, the number of the first card slots and the second card slots can both be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc.

[0118] In some embodiments, 5 first card slots arranged in sequence are provided on the first pressing plate 17, which are respectively a first card slot A, a first card slot B, a first card slot C, a first card slot D, and a first card slot E. 5 second card slots arranged in sequence are provided on the second pressing plate, which are respectively a second card slot A, a second card slot B, a second card slot C, a second card slot D, and a second card slot E. The first card slot A is arranged opposite to the second card slot A, the first card slot B is arranged opposite to the second card slot B, the first card slot C is arranged opposite to the second card slot C, the first card slot D is arranged opposite to the second card slot D, and the first card slot E is arranged opposite to the second card slot E.

[0119] In some embodiments, when the nucleic acid extraction and detection kit is a multi-well plate, the direction from the first card slot to the second card slot is the extending direction of the well positions of the multi-well plate, and this direction is also the first direction. Both ends of the extending direction of the well positions of the multi-well plate are detachably clamped in the first card slot and the second card slot. That is, both ends of the multi-well plate are respectively clamped in the first card slot A and the second card slot A.

[0120] In the present application, the fluorescence channel assembly is arranged on the first bottom plate 1, and the optical fiber of the fluorescence channel assembly extends into the detection well position, so as to perform PCR detection on the sample in the nucleic acid extraction and detection kit.

[0121] Furthermore, the fluorescence channel assembly includes an optical fiber sensor, and the optical fiber at one end of the optical fiber sensor extends into the detection well position, so as to perform PCR detection on the sample in the nucleic acid extraction and detection kit.

[0122] In the present application, the temperature control assembly includes a heating unit 18 and a cooling unit 19. The heating unit 18 is arranged directly below the fixed sleeve 25, and the cooling unit 19 is arranged on one side of the heating unit 18, and is used to cool the heating unit 18.

[0123] In the present application, the system further includes a display mechanism, and the display mechanism is connected to the control mechanism and is used to issue commands to the control mechanism.

[0124] The control mechanism sends commands to the detection kit carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the drive assembly, and the fluorescence channel assembly through the display mechanism, and transmits the data of the detection kit carrier assembly, the extraction and purification pipetting assembly, the temperature control assembly, the drive assembly, and the fluorescence channel assembly to the display mechanism.

[0125] In the present application, the system further includes a protection assembly, and the protection assembly includes an ultraviolet disinfection unit, an illumination unit, and an operation warning unit. The ultraviolet disinfection unit is used to disinfect the areas in the system that need to be disinfected, the illumination assembly is used to illuminate the areas that need illumination, and the operation warning unit is used to monitor each component in the system in real time, give risk warnings and perform linkage control to ensure the safe and stable operation of the system and prevent accidents.

[0126] Embodiment

[0127] The materials and test methods used in the embodiments of the present application are generally and / or specifically described. In the following embodiments, if there is no other special description, % represents wt%, that is, weight percentage. For the reagents or instruments whose manufacturers are not indicated, they are all conventional reagent products that can be obtained through commercial purchase.

[0128] Example 1

[0129] The full-automatic nucleic acid processing system of this embodiment includes a control component, a detection cartridge carrier component, an extraction and purification pipetting component, a temperature control component, a driving component, a fluorescence channel component, and a display component. The control mechanism sends commands to the detection cartridge carrier component, the extraction and purification pipetting component, the temperature control component, the driving component, and the fluorescence channel component through the display mechanism, and transmits the data of the detection cartridge carrier component, the extraction and purification pipetting component, the temperature control component, the driving component, and the fluorescence channel component to the display mechanism.

[0130] The detection cartridge carrier component includes a first fixing part and four groups of heating fixing parts. The first fixing part includes a first fixing member 15 and a second fixing member 16 which are oppositely arranged. Between the first fixing member 15 and the second fixing member 16, twelve detection holes and twelve groups of the heating fixing parts are arranged along the first direction. Each group of the heating fixing parts includes four fixing sleeves 25 which are arranged at intervals. The first fixing member 15 and the second fixing member 16 are respectively used to limit the two ends of the nucleic acid extraction detection cartridge, and the fixing sleeve 25 is used to limit a part of the nucleic acid extraction detection cartridge; when the nucleic acid extraction detection cartridge has multiple holes, the holes that need to be heated can be placed in the fixing sleeve 25. The height of the first fixing member 15 and the second fixing member 16 is higher than the height of the fixing sleeve 25. The side wall of the first fixing member 15 facing the second fixing member 16 has twelve groups of first fixing grooves recessed into its interior, and the side wall of the second fixing member 16 facing the first fixing member 15 has twelve groups of second fixing grooves recessed into its interior. The twelve groups of first fixing grooves and the twelve groups of second fixing grooves are arranged oppositely. The two ends of the nucleic acid extraction detection cartridge can be respectively clamped on the first fixing grooves and the second fixing grooves, so as to limit the position of the nucleic acid extraction detection cartridge.

[0131] The extraction and purification pipetting assembly includes a set of magnetic bar rack assemblies 24 and two sets of multi-channel pipetting assemblies. The two sets of multi-channel pipetting assemblies are arranged in parallel, and the magnetic bar rack assembly 24 is disposed between the two sets of multi-channel pipetting assemblies. The magnetic bar rack assembly 24 is vertically movably disposed above the test cartridge carrier assembly and is used for oscillation and / or magnetic absorption during the nucleic acid extraction process. The multi-channel pipetting assembly is vertically movably disposed above the test cartridge carrier assembly and is used to drive the syringe to perform pipetting during the nucleic acid extraction process. A set of magnetic bar rack assemblies 24 includes 12 magnetic bars and 12 magnetic bar sleeves. The 12 magnetic bars are equidistantly arranged, and a partial area of the magnetic bars is movably disposed within the magnetic bar sleeves. Each set of multi-channel pipetting assemblies includes an injection cylinder driving part assembly 22 and 12 piston driving parts 21. The injection cylinder driving part assembly 22 includes 12 spaced injection cylinder driving parts. The interiors of the 12 injection cylinder driving parts are independent of each other and do not communicate with each other. The exteriors of the 12 injection cylinder driving parts are connected together to form an integral body. A partial area of the piston driving part 21 is movably disposed within the injection cylinder driving part.

[0132] The system further includes a first bottom plate 1, a second bottom plate 2, a third bottom plate 3, a fourth bottom plate 4, four support columns 14, a first bracket 5, a second bracket 6, a fourth bracket 7, and a fifth bracket 9. The fourth bottom plate 4 is fixedly connected to the second bottom plate 2 through the four support columns 14. The first bracket 5, the second bracket 6, two sets of fifth brackets 9, and two sets of fourth brackets 7 are all connected to the fourth bottom plate 4. The driving assembly includes a first driving assembly, a second driving assembly, a third driving assembly, a fourth driving assembly, and a fifth driving assembly.

[0133] The first driving assembly includes a first driving motor 8, a first driving shaft, a first slider, and a first slide rail. The first driving motor 8 and the first slide rail are disposed on the second bottom plate 2. The first slider is disposed at the bottom of the first bottom plate 1. The first driving motor 8 is connected to the first driving shaft, and the first driving shaft is connected to the slider. The first driving motor 8 can drive the first driving shaft to perform a reciprocating motion in the first direction, thereby driving the first slider to perform a reciprocating motion in the first direction on the first chute, and further driving the first bottom plate 1 and each part structure on the first bottom plate 1 to perform a reciprocating motion in the first direction.

[0134] The second driving assembly includes a second driving motor 20 and a second driving shaft. The second driving motor 20 is connected to the second driving shaft, and the second driving shaft is connected to the first bracket 5. Thus, when the second driving motor 20 drives the second driving shaft to perform a lifting movement in the second direction, the first bracket 5 will also perform a lifting movement in the second direction, and further drive the magnetic bar to perform a lifting movement in the second direction.

[0135] The third driving assembly includes a third driving motor 10 and a third driving shaft. The third driving motor 10 is connected to the third driving shaft, and the third driving shaft is connected to the second bracket 6. Therefore, when the third driving motor 10 drives the third driving shaft to move up and down along the second direction, the second bracket 6 will also move up and down along the second direction, thereby driving the magnetic rod sleeve to move up and down along the second direction.

[0136] The first bracket 5 includes a first fixed bracket and a first connecting bracket, the first fixed bracket is connected to the second driving shaft, and the first connecting bracket is connected to the magnetic rod. The second bracket 6 includes a second fixed bracket and a second connecting bracket, the second fixed bracket is connected to the third driving shaft, the second connecting bracket is connected to the magnetic rod sleeve, the first connecting bracket has a first through hole, the second connecting bracket penetrates the first through hole so that the first connecting bracket is located above the second connecting bracket, and the magnetic rod is located directly above the magnetic rod sleeve. A first limiting cylinder surrounding the first through hole is provided on the first connecting bracket, the second connecting bracket penetrates the first limiting cylinder and the first through hole, when the second connecting bracket is lifted and moved, the first connecting bracket is not affected, and the first limiting cylinder can limit the displacement or shaking of the second connecting bracket when it is lifted and moved.

[0137] The fourth drive assembly includes a fourth drive motor 11 and a fourth drive shaft, wherein the fourth drive motor 11 is connected to the fourth drive shaft, and the fourth drive motor 11 drives the fourth drive shaft to move up and down along the second direction. The fourth drive motor 11 is connected to the fourth bottom plate 4, and the fourth drive shaft is connected to the fourth bracket 7. The fourth drive motor 11 drives the fourth drive shaft to move up and down along the second direction, thereby driving the fourth bracket 7, the syringe drive unit, and the syringe to move up and down along the second direction.

[0138] The fifth drive assembly includes a fifth drive motor 12 and a fifth drive shaft, wherein the fifth drive motor 12 is connected to the fifth drive shaft, and the fifth drive motor 12 drives the fifth drive shaft to move up and down along the second direction. The fifth drive motor 12 is connected to the fourth bracket 7, and the fifth drive shaft is connected to the fifth bracket 9. The fifth drive motor 12 drives the fifth drive shaft to move up and down along the second direction, thereby driving the fifth bracket 9, the piston driving part 21 and the piston to move up and down along the second direction.

[0139] The fifth bracket 9 includes a fifth fixed bracket and a fifth limiting sleeve. Both ends of the fifth fixed bracket are provided with fifth through holes, and the fifth limiting sleeve is arranged around the fifth through holes. Twelve of the pistons are connected to the fifth fixed bracket along the second direction. The fourth bracket 7 includes a fourth fixed bracket and a fourth connecting bracket. The fourth fixed bracket is connected to the fourth drive shaft. The fourth fixed bracket and the fourth base plate 4 are connected in a liftable manner through a fourth limiting slide rail. The fourth connecting bracket passes through the fifth limiting sleeve and the fifth through hole and is connected to the syringe driving part assembly 22. When the fourth connecting bracket moves up and down, it does not affect the fifth fixed bracket, and the fifth limiting sleeve can limit the offset or sway of the fourth connecting bracket during the up and down movement. Two sets of the fourth brackets 7 are arranged in parallel, and two sets of the fifth brackets 9 are arranged in parallel.

[0140] The sixth driving assembly includes a sixth driving motor 13 and a sixth drive shaft. The sixth driving motor 13 is connected to the sixth drive shaft, and the sixth driving motor 13 drives the sixth drive shaft to move up and down along the second direction. The sixth driving motor 13 is fixedly connected to the fourth base plate 4, and the sixth drive shaft is connected to the third base plate 3. By driving the sixth drive shaft to move up and down along the second direction through the sixth driving motor 13, the third base plate 3 is driven to move up and down along the second direction.

[0141] The third base plate 3 is arranged between the fourth base plate 4 and the first base plate 1. A sliding sleeve is arranged on the support column 14, and the third base plate 3 is sleeved on the sliding sleeve, so that the third base plate 3 slides on the support column 14 through the sliding sleeve. The third base plate 3 is located above the test cartridge carrier assembly, and the middle of the third base plate 3 has an opening. When the multi-channel pipetting assembly and the magnetic rod rack assembly 24 move along the second direction, they can extend into the opening and then into the nucleic acid extraction test cartridge. One side surface of the third base plate 3 facing the test cartridge carrier assembly is provided with a first pressing plate 17 and a second pressing plate. The first pressing plate 17 is located above the first fixing member 15, and the second pressing plate is located above the second fixing member 16. One side surface of the first pressing plate 17 facing the nucleic acid extraction test cartridge is provided with 12 first clamping grooves, and one side surface of the second pressing plate facing the nucleic acid extraction test cartridge is provided with 12 second clamping grooves. The first clamping grooves and the second clamping grooves are used for clamping the nucleic acid extraction test cartridge.

[0142] The fluorescence channel assembly is arranged on the first base plate 1. The fluorescence channel assembly includes an optical fiber sensor, and the optical fiber of the optical fiber sensor extends into the detection hole position to perform PCR detection on the sample in the nucleic acid extraction test cartridge.

[0143] The temperature control component includes a heating unit 18 and a cooling unit 19. The heating unit 18 is disposed directly below the fixing sleeve 25, and the cooling unit 19 is disposed on one side of the heating unit 18 for cooling the heating unit 18.

[0144] When the system of this embodiment is in use, the nucleic acid extraction and detection kit is placed in the detection kit carrier assembly and fixed by the first fixing member 15 and the second fixing member 16. Then, the sixth driving component drives the third bottom plate 3 to descend, so that the first pressing plate 17 and the second pressing plate press on the top of the nucleic acid extraction and detection kit. Then, the plasma sample is injected into the nucleic acid extraction and detection kit. Then, the second driving component, the third driving component, the fourth driving component, and the fifth driving component are used to adjust the lifting of the magnetic rod holder assembly 24 and the multi-channel pipetting assembly to perform nucleic acid extraction and purification. After the nucleic acid extraction and purification are completed, the optical fiber of the fluorescence channel component performs PCR detection through the detection hole position. For the full-automatic nucleic acid methylation processing system described in this application, the entire process from the plasma sample entering the device to obtaining the PCR detection result of the target DNA is completely completed by the device without manual intervention, and high automation of nucleic acid methylation extraction and detection can be achieved. Moreover, each mechanism in this application has strong independence. While the mechanisms can cooperate with each other, a single mechanism can also complete the corresponding work; the relative positions of all the mechanisms can change according to the limitation of the placement space, and the relative positions are flexible and variable.

[0145] Although the embodiments of the present application have been described above in conjunction with the accompanying drawings, the present application is not limited to the above specific embodiments and application fields. The above specific embodiments are merely illustrative and guiding, rather than restrictive. Those of ordinary skill in the art can also make many forms under the inspiration of this specification and without departing from the scope protected by the claims of the present application, and these all belong to the scope of protection of the present application.

Claims

1. An automatic nucleic acid processing system, wherein, It includes a control component, a detection cassette carrier component, an extraction and purification pipetting component, a temperature control component, a drive component, and a fluorescence channel component. Among them, the control component controls the detection cassette carrier component, the extraction and purification pipetting component, the temperature control component, the drive component, and the fluorescence channel component respectively; the detection cassette carrier component is used for placing a nucleic acid extraction and detection cassette; the detection cassette carrier component includes a first fixing part and at least one set of heating fixing parts. The first fixing part includes a first fixing member and a second fixing member arranged oppositely. Between the first fixing member and the second fixing member, at least one set of the heating fixing parts is provided. Each set of the heating fixing parts includes at least two fixing sleeves arranged at intervals. The first fixing member and the second fixing member are respectively used for restricting both ends of the nucleic acid extraction and detection cassette, and the fixing sleeves are used for restricting part of the area of the nucleic acid extraction and detection cassette; the extraction and purification pipetting component is arranged above the detection cassette carrier component in a liftable manner and is used for pipetting, oscillating, and / or magnetic attraction during the nucleic acid extraction process; the drive component is used for driving the detection cassette carrier component to move back and forth in a first direction and driving the extraction and purification pipetting component to lift and lower in a second direction; the temperature control component is arranged below the fixing sleeve and is used for providing temperature conditions for the nucleic acid extraction and detection cassette in the fixing sleeve; the fluorescence channel component is used for performing PCR detection on the sample in the nucleic acid extraction and detection cassette.

2. The system according to claim 1, wherein The side wall of the first fixing member facing the second fixing member has at least one set of first fixing grooves recessed into its interior; the side wall of the second fixing member facing the first fixing member has at least one set of second fixing grooves recessed into its interior.

3. The system according to claim 2, wherein, A detection hole position is also arranged between the first fixing member and the second fixing member. The detection hole position is close to one side of the first fixing member. The number of the heating fixing parts, the number of the detection hole positions, the number of the first fixing grooves, and the number of the second fixing grooves are equal.

4. The system according to claim 3, wherein The system also includes a first bottom plate. The drive component includes a first drive component. The temperature control component is arranged on the first bottom plate. The first drive component is used for driving the first bottom plate to move back and forth in a first direction, and further driving the temperature control component and the detection cassette carrier component to move back and forth in a first direction.

5. The system according to claim 4, wherein The system also includes a second bottom plate. The first drive component is arranged on the second bottom plate. The first bottom plate moves on the second bottom plate in a first direction.

6. The system according to claim 1, wherein, The extraction and purification pipetting component includes a magnetic rod rack component and a multi-channel pipetting component. The magnetic rod rack component is arranged above the detection cassette carrier component in a liftable manner and is used for oscillating and / or magnetic attraction during the nucleic acid extraction process. The multi-channel pipetting component is arranged above the detection cassette carrier component in a liftable manner and is used for driving a syringe to perform pipetting during the nucleic acid extraction process.

7. The system according to claim 6, wherein, The system also includes a fourth bottom plate, a first bracket, a second bracket, a fourth bracket, and a fifth bracket. The fourth bottom plate is fixedly connected to the second bottom plate through a support column. The first bracket, the second bracket, the fourth bracket, and the fifth bracket are all connected to the fourth bottom plate.

8. The system according to claim 7, wherein, The magnetic rod holder assembly includes a plurality of magnetic rods arranged in an array and magnetic rod sleeves. The magnetic rods are located above the magnetic rod sleeves and can move downward and extend into the magnetic rod sleeves.

9. The system according to claim 8, wherein, The driving assembly further includes a second driving assembly and a third driving assembly. The second driving assembly is used to drive the magnetic rods to move up and down in a second direction, and the third driving assembly is used to drive the magnetic rod sleeves to move up and down in the second direction; The magnetic rod holder assembly is located above the nucleic acid extraction and detection box. The second driving assembly and the third driving assembly are used to drive the magnetic rod holder assembly or the magnetic rod sleeves to move above the nucleic acid extraction and detection box, and can move downward to make the magnetic rod holder assembly or the magnetic rod sleeves extend into the nucleic acid extraction and detection box.

10. The system according to claim 8 or 9, wherein, m columns of magnetic rods are arranged on the first bracket, the spacing between the m columns of magnetic rods is adjustable, each column has n magnetic rods, and the spacing between the n magnetic rods is adjustable; m columns of magnetic rod sleeves are arranged on the second bracket, the spacing between the m columns of magnetic rod sleeves is adjustable, each column has n magnetic rod sleeves, and the spacing between the n magnetic rod sleeves is adjustable; Where m≥1 and n≥2.

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