Four-channel chip fluid control system for stem cell detection

Abstract

The invention discloses a four-channel chip fluid control system for stem cell detection, and relates to the technical field of stem cell detection equipment. The device comprises a detection chip, and four interfaces, namely a first interface, a second interface, a third interface and a fourth interface, are formed in the peripheral side walls of the detection chip; the first interface is connected with a three-way pipe I, one passage of the three-way pipe I is sequentially connected with a first straight-through electromagnetic valve and a detection container, and the other passage of the three-way pipe I is connected with a three-way pipe II. According to the invention, automatic operation of functions of pre-filling of detection liquid, stem cell detection, pipeline cleaning, system solution discharge, chip oscillation blockage removal and the like can be realized, manual operation is reduced, and the automation degree of the detection process is improved; meanwhile, through the design of independent pipelines and containers and the effective cleaning process, the risk of cross contamination between different samples and reagents is reduced, and the continuity and accuracy of the detection process are ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell detection equipment, and particularly to a four-channel chip fluid control system for stem cell detection. Background Technique

[0002] With the in-depth research on stem cells, stem cell detection is increasingly widely used in regenerative medicine, drug screening, and basic biological research. The introduction of microfluidic technology can improve the throughput and accuracy of stem cell detection. The key lies in being able to precisely and efficiently control the fluid, allowing the cells to be detected to pass through the detection area at high speed and in sequence. The existing representative detection technology is represented by flow cytometry. The detection area usually adopts a unidirectional flow design with a single inlet and a single outlet, and the fluid control principle is relatively simple. However, in high-throughput electrical detection scenarios, to reduce electrical signal noise, the detection chip usually includes two fluid inlets and fluid outlets. For the functional requirements of chip pre-filling, detection, cleaning, etc., it is necessary to control multiple liquids to achieve complex flow control in the chip. The existing fluid systems cannot meet the above control requirements. Therefore, the present invention proposes a four-channel chip fluid control system for stem cell detection. Summary of the Invention

[0003] The purpose of the present invention is to provide a four-channel chip fluid control system for stem cell detection, which can improve the degree of detection automation, achieve high-throughput and high-efficiency stem cell detection, reduce the risk of cross-contamination, and improve the accuracy and flexibility of experiments.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A four-channel chip fluid control system for stem cell detection, including a detection chip, and four interfaces are provided on the peripheral side walls of the detection chip, namely a first interface, a second interface, a third interface, and a fourth interface;

[0005] A three-way pipe one is connected to the first interface. One path of the three-way pipe one is sequentially connected to a first direct solenoid valve and a detection container, and the other path is connected to a three-way pipe two. One path of the three-way pipe two is connected to the fourth interface of the detection chip, and the other path is connected to a first peristaltic pump. A three-way solenoid valve is connected to the first peristaltic pump, and the two inlets of the three-way solenoid valve are respectively connected to a detection liquid container and a cleaning liquid container;

[0006] A third direct solenoid valve and a three-way pipe three are sequentially connected to the third interface. One path of the three-way pipe three is sequentially connected to a second peristaltic pump and a waste liquid container, and the other path is connected to the second interface of the detection chip.

[0007] Further, an inlet pressure sensor and an outlet pressure sensor are also provided on the detection chip, and the numerical difference between the inlet pressure sensor and the outlet pressure sensor is used to judge the blockage condition of the detection chip.

[0008] Further, the detection liquid container is filled with physiological saline, PBS solution or cell culture medium, and the cleaning liquid container is filled with deionized water.

[0009] Further, a second direct solenoid valve is also connected between the three-way pipe two and the fourth interface.

[0010] Further, the detection liquid container, the three-way solenoid valve, the first peristaltic pump, the three-way pipe two, the three-way pipe one, the first direct solenoid valve and the detection container form a detection container pipeline pre-filling module, which is used to realize the liquid filling of the passage between the detection liquid container and the detection container, and the fluid pre-filling flow rate is 0.5 to 2 mL / min.

[0011] Further, the detection liquid container, the three-way solenoid valve, the first peristaltic pump, the three-way pipe two, the three-way pipe one, the detection chip, the second direct solenoid valve, the third direct solenoid valve, the three-way pipe three, the second peristaltic pump and the waste liquid container form a detection chip pipeline pre-filling module, which is used to realize the liquid pre-filling of the pipeline between the detection chip and the waste liquid container, and the fluid pre-filling flow rate is 0.5 to 2 mL / min.

[0012] Further, the detection container, the first direct solenoid valve, the three-way pipe one, the detection chip, the three-way pipe three, the second peristaltic pump and the waste liquid container form a stem cell detection module;

[0013] The stem cell detection module includes two working conditions. One is the rapid sample injection working condition of the detection solution, which is used to realize the rapid flow of the stem cell suspension to be detected in the detection container to the detection area of the detection chip, and the fluid flow rate is 0.5 to 2 mL / min at this time; the other is the stem cell detection working condition, which is used to realize the uniform and sequential passage of the stem cell suspension through the detection area of the detection chip, and the fluid flow rate is 0.1 to 1 mL / min at this time.

[0014] Further, the cleaning liquid container, the three-way solenoid valve, the first peristaltic pump, the three-way pipe two, the three-way pipe one, the first direct solenoid valve and the detection container form a detection container pipeline cleaning module, which is used to realize the liquid cleaning of the passage between the cleaning liquid container and the detection container, and the fluid cleaning flow rate is 0.5 to 5 mL / min at this time.

[0015] Further, the cleaning liquid container, three-way solenoid valve, first peristaltic pump, second three-way pipe, first three-way pipe, detection chip, second direct solenoid valve, third direct solenoid valve, third three-way pipe, second peristaltic pump, and waste liquid container form a detection chip pipeline cleaning module, which is used to realize the liquid cleaning of the pipeline between the detection chip and the waste liquid container. At this time, the fluid cleaning flow rate is 0.5 to 5 mL / min.

[0016] Further, the detection container, first direct solenoid valve, first three-way pipe, detection chip, second three-way pipe, second direct solenoid valve, third direct solenoid valve, third three-way pipe, second peristaltic pump, and waste liquid container form a solution discharge module, which is used to realize the discharge of the solution in the overall pipeline, and the discharge flow rate is 0.5 to 2 mL / min.

[0017] The present invention has at least the following beneficial effects:

[0018] By integrating components such as a stem cell detection chip, peristaltic pump, solenoid valve, and three-way pipe, the present invention can realize the automated operation of functions such as pre-filling of the detection liquid, stem cell detection, pipeline cleaning, system solution discharge, and chip oscillation for blockage removal, reducing manual operation and improving the automation degree of the detection process; at the same time, through independent pipeline and container design and effective cleaning processes, the risk of cross-contamination between different samples and reagents is reduced, and the pressure sensor of the detection chip can monitor the blockage situation in real time and automatically start the oscillation function for blockage removal to ensure the continuity and accuracy of the detection process.

[0019] Of course, it is not necessary for any product implementing the present invention to simultaneously achieve all the above-mentioned advantages. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic structural diagram of the overall system of the present invention.

[0021] Figure 2 It is a schematic interface diagram of the structure of the detection chip of the present invention.

[0022] Figure 3 It is a schematic structural diagram of the detection container pipeline pre-filling module of the present invention.

[0023] Figure 4 It is a schematic structural diagram of the detection chip pipeline pre-filling module of the present invention.

[0024] Figure 5 It is a schematic structural diagram of the stem cell detection module of the present invention.

[0025] Figure 6 It is a schematic structural diagram of the detection container pipeline cleaning module of the present invention.

[0026] Figure 7This is a schematic structural diagram of the pipeline cleaning module of the detection chip of the present invention. Figure 8 This is a schematic structural diagram of the solution discharge module of the present invention.

[0027] Reference numerals:

[0028] 10. Detection chip; 1001. First interface; 1002. Second interface; 1003. Third interface; 1004. Fourth interface; 1005. Detection inlet pressure sensor; 1006. Detection outlet pressure sensor; 20. Detection container; 30. Detection liquid container; 40. Cleaning liquid container; 50. Waste liquid container; 60A. First peristaltic pump; 60B. Second peristaltic pump; 70. Three-way solenoid valve; 80A. First straight-through solenoid valve; 80B. Second straight-through solenoid valve; 80C. Third straight-through solenoid valve; 90A. First three-way pipe; 90B. Second three-way pipe; 90C. Third three-way pipe. Specific embodiments

[0029] Next, the technical solutions in the embodiments of the present disclosure will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present disclosure. Obviously, the described embodiments are only a part of the embodiments of the present disclosure, rather than all the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present disclosure.

[0030] Please refer to Figure 1 and Figure 2 The present invention provides a technical solution: a four-channel chip fluid control system for stem cell detection, including a stem cell detection chip 10, a detection container 20, a detection liquid container 30, a cleaning liquid container 40, a waste liquid container 50, a first peristaltic pump 60A, a second peristaltic pump 60B, a three-way solenoid valve 70, a first straight-through solenoid valve 80A, a second straight-through solenoid valve 80B, a third straight-through solenoid valve 80C, a first three-way pipe 90A, and a second three-way pipe 90B;

[0031] Four interfaces are provided on the peripheral side walls of the detection chip 10, which are respectively a first interface 1001, a second interface 1002, a third interface 1003, and a fourth interface 1004;

[0032] A tee 90A is connected to the first interface 1001. One path of the tee 90A is sequentially connected to a first direct solenoid valve 80A and a detection container 20. Another path is connected to a tee 90B. One path of the tee 90B is connected to the fourth interface 1004 of the detection chip 10, and a second direct solenoid valve 80B is also connected between the tee 90B and the fourth interface 1004. Another path is connected to a first peristaltic pump 60A. A three-way solenoid valve 70 is connected to the first peristaltic pump 60A, and two inlets of the three-way solenoid valve 70 are respectively connected to a detection liquid container 30 and a cleaning liquid container 40;

[0033] A third direct solenoid valve 80C and a tee 90C are sequentially connected to the third interface 1003. One path of the tee 90C is sequentially connected to a second peristaltic pump 60B and a waste liquid container 50. Another path is connected to the second interface 1002 of the detection chip 10.

[0034] Regarding the technical solution of this embodiment, as Figure 2 shown, the stem cell detection chip 1010 in this embodiment includes a first interface 1001 of the detection chip 10, a second interface 1002 of the detection chip 10, a third interface 1003 of the detection chip 10, a fourth interface 1004 of the detection chip 10, a detection inlet pressure sensor 1005; a detection outlet pressure sensor 1006, where the first interface 1001 and the fourth interface 1004 are two inlets, and the second interface 1002 and the third interface 1003 are two outlets. The numerical difference between the detection inlet pressure sensor 1005 and the detection outlet pressure sensor 1006 is used to judge the blockage condition of the detection chip 10.

[0035] Regarding the technical solution of this embodiment, the detection liquid container 30 is filled with normal saline, PBS solution or cell culture medium, and the cleaning liquid container 40 is filled with deionized water. This embodiment does not make specific limitations here and can be selected according to actual situations.

[0036] As Figure 3As shown, the detection liquid container 30, three-way solenoid valve 70, first peristaltic pump 60A, second three-way pipe 90B, first three-way pipe 90A, first direct solenoid valve 80A and detection container 20 constitute the pre-filling module for the pipeline of the detection container 20, which is used to realize the liquid filling of the passage between the detection liquid container 30 and the detection container 20. In the pre-filling condition of the pipeline of the detection container 20, the three-way solenoid valve 70 is switched to connect to the detection liquid container 30, the first peristaltic pump 60A rotates clockwise, the first direct solenoid valve 80A is turned on, the second direct solenoid valve 80B, the third direct solenoid valve 80C and the second peristaltic pump 60B are closed, and the detection liquid is filled into the detection container 20 under the negative pressure suction of the first peristaltic pump 60A to complete the filling of the pipeline of the detection container 20. The pre-filling flow rate of the fluid can be set to 0.5 to 2 mL / min. In this embodiment, the rotation speed of the peristaltic pump is 200 rpm / min and the filling flow rate is 2 mL / min.

[0037] As Figure 4 As shown, the detection liquid container 30, three-way solenoid valve 70, first peristaltic pump 60A, second three-way pipe 90B, first three-way pipe 90A, detection chip 10, second direct solenoid valve 80B, third direct solenoid valve 80C, third three-way pipe 90C, second peristaltic pump 60B and waste liquid container 50 constitute the pre-filling module for the pipeline of the detection chip 10, which is used to realize the pre-filling of the liquid in the pipeline between the detection chip 10 and the waste liquid container 50. In the pre-filling condition of the pipeline of the detection chip 10, the three-way solenoid valve 70 is switched to connect to the detection liquid container 30, the first peristaltic pump 60A and the second peristaltic pump 60B rotate counterclockwise at the same time, the first direct solenoid valve 80A is closed, the second direct solenoid valve 80B and the third direct solenoid valve 80C are opened, and the detection liquid flows into the internal pipeline of the cell detection chip 10 through the second direct solenoid valve 80B and the first three-way pipe 90A to complete the pre-filling of the pipeline of the detection chip 10. The pre-filling flow rate of the fluid can be set to 0.5 to 2 mL / min. In this embodiment, the rotation speeds of the first peristaltic pump 60A and the second peristaltic pump 60B are both 200 rpm / min, the filling flow rate is 2 mL / min, and the excess filling liquid flows into the waste liquid container 50.

[0038] After the pre-filling function of the detection liquid is completed, the tester adds the cell liquid to be detected into the detection container 20 and starts the stem cell detection function.

[0039] Regarding the technical solution of this embodiment, as Figure 5As shown, the detection container 20, the first direct solenoid valve 80A, the first three-way pipe 90A, the detection chip 10, the third three-way pipe 90C, the second peristaltic pump 60B, and the waste liquid container 50 constitute the stem cell detection module. In the stem cell detection function, the second peristaltic pump 60B rotates clockwise, the first direct solenoid valve 80A and the third direct solenoid valve 80C are opened, and other solenoid valves and peristaltic pumps are closed. In this function, the solution to be detected is sucked out from the detection container 20, flows through the first direct solenoid valve 80A, the first three-way pipe 90A, the first interface 1001 of the detection chip 10, the chip detection area inside the detection chip 10, the second interface 1002 of the detection chip 10, the third three-way pipe 90C, the second peristaltic pump 60B, and flows into the waste liquid container 50. The stem cell detection function is divided into two working conditions. The first one to start is the rapid sample injection working condition, and the corresponding rotation speed of the second peristaltic pump 60B is 100 rpm / min, and the fluid flow rate is 1 mL / min. The purpose is to quickly circulate the solution to be detected to the detection area inside the detection chip 10. Then the stem cell detection working condition is started. In this embodiment, the concentration of the detection solution is about 1e 6 cells / mL, and the detection chip 10 of model T-60 is used. The corresponding detection flow rate is 200 μL / min, and the rotation speed of the second peristaltic pump 60B is 20 rpm / min.

[0040] Furthermore, in the stem cell detection working condition, since the stem cells of the detected object are prone to adhere to the inner wall surface of the microchannel in the detection area, resulting in an increase in the flow resistance of the device and bringing errors to the detection results. In this embodiment, the system will automatically obtain the detection inlet pressure sensor 1005 and the detection outlet pressure sensor 1006, calculate the pressure difference between the two, and compare the data with the pressure range corresponding to 200 μL / min and the T-60 model chip. When the detected pressure value exceeds the rated working condition pressure range, it is determined that the microchannel of the detection chip 10 is blocked. The system automatically starts the chip oscillation blockage removal function. In this function, the switch states of each component are the same as those in the stem cell detection function, but the second peristaltic pump 60B will start a clockwise and counterclockwise alternating working mode. In this embodiment, the rotation speed is 300 rpm / min, the corresponding maximum oscillation flow rate is 3 mL / min, and the oscillation period is 10 times. After oscillation, the sensor pressure difference and the rated pressure value are compared again. If it returns to normal, the stem cell detection function continues. If the sensor pressure is still unqualified, it is prompted to replace the detection chip 10.

[0041] After the test is completed, the system automatically starts the pipeline cleaning function, including the pipeline cleaning working condition of the detection container 20 and the pipeline cleaning working condition of the detection chip 10.

[0042] Regarding the technical solution of this embodiment, such as Figure 6As shown, the cleaning liquid container 40, the three-way solenoid valve 70, the first peristaltic pump 60A, the second three-way pipe 90B, the first three-way pipe 90A, the first direct solenoid valve 80A and the detection container 20 constitute the detection container 20 pipeline cleaning module, which is used to realize the liquid cleaning of the pipeline between the cleaning liquid container 40 and the detection container 20. Under the condition of cleaning the pipeline of the detection container 20, the three-way solenoid valve 70 is switched to connect to the cleaning liquid container 40, the first peristaltic pump 60A rotates clockwise, the first direct solenoid valve 80A is turned on, the second direct solenoid valve 80B, the third direct solenoid valve 80C and the second peristaltic pump 60B are closed, and the cleaning liquid is filled into the detection container 20 under the negative pressure suction of the first peristaltic pump 60A to complete the cleaning of the pipeline of the detection container 20. At this time, the fluid cleaning flow rate can be set to 0.5 to 5 mL / min. In this embodiment, the rotation speed of the peristaltic pump is 500 rpm / min and the cleaning flow rate is 5 mL / min.

[0043] Further, as Figure 7 shown, the cleaning liquid container 40, the three-way solenoid valve 70, the first peristaltic pump 60A, the second three-way pipe 90B, the first three-way pipe 90A, the detection chip 10, the second direct solenoid valve 80B, the third direct solenoid valve 80C, the third three-way pipe 90C, the second peristaltic pump 60B and the waste liquid container 50 constitute the detection chip 10 pipeline cleaning module, which is used to realize the liquid cleaning of the pipeline between the detection chip 10 and the waste liquid container 50. Under the condition of cleaning the pipeline of the detection chip 10, the three-way solenoid valve 70 is switched to connect to the cleaning liquid container 40, the first peristaltic pump 60A and the second peristaltic pump 60B rotate counterclockwise at the same time, the first direct solenoid valve 80A is closed, the second direct solenoid valve 80B and the third direct solenoid valve 80C are opened, and the cleaning liquid flows into the internal pipeline of the stem cell detection chip 10 through the second direct solenoid valve 80B and the first three-way pipe 90A to complete the cleaning of the pipeline of the detection chip 10. At this time, the fluid cleaning flow rate can be set to 0.5 to 5 mL / min. In this embodiment, the rotation speeds of the first peristaltic pump 60A and the second peristaltic pump 60B are both 500 rpm / min, the filling flow rate is 5 mL / min, and the excess cleaning liquid flows into the waste liquid container 50.

[0044] Regarding the technical solution of this embodiment, as Figure 8As shown, the detection container 20, the first direct solenoid valve 80A, the first three-way pipe 90A, the detection chip 10, the second three-way pipe 90B, the second direct solenoid valve 80B, the third direct solenoid valve 80C, the third three-way pipe 90C, the second peristaltic pump 60B and the waste liquid container 50 constitute the solution discharge module. After the pipeline cleaning function is completed, the system starts the system solution discharge function to discharge the cleaning liquid remaining in the pipeline. In this function, the first direct solenoid valve 80A, the second direct solenoid valve 80B, and the third direct solenoid valve 80C are opened, the first peristaltic pump 60A is closed, and the second peristaltic pump 60B rotates clockwise; under the negative pressure of the second peristaltic pump 60B, air enters from the detection container 20 channel, and the liquid in the pipeline in the system is discharged in sequence and completely.

[0045] During the operation of the present invention, functions such as pre-filling of the detection liquid, stem cell detection, pipeline cleaning, system solution discharge, and chip oscillation blockage removal are automatically started, effectively improving the detection efficiency; at the same time, by having the cleaning and solution discharge functions, the risk of cross-contamination between different samples and reagents is reduced. The pressure sensor of the detection chip 10 can monitor the blockage situation in real time and automatically start the oscillation blockage removal function to ensure the continuity and accuracy of the detection process.

[0046] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0047] For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. When an element is referred to as "assembled on", "installed on", "fixed on" or "set on" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are only for the purpose of illustration and do not represent the only implementation.

[0048] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the invention. The scope of the invention is defined by the appended claims and their equivalents.

[0049] In the description of this specification, the descriptions referring to the terms "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present disclosure. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

Claims

1. A four-channel chip fluid control system for stem cell detection, comprising a detection chip (10), characterized in that, Four interfaces are provided on the peripheral side walls of the detection chip (10), namely a first interface (1001), a second interface (1002), a third interface (1003), and a fourth interface (1004); A first three-way pipe (90A) is connected to the first interface (1001). One path of the first three-way pipe (90A) is sequentially connected to a first direct solenoid valve (80A) and a detection container (20), and the other path is connected to a second three-way pipe (90B). One path of the second three-way pipe (90B) is connected to the fourth interface (1004) of the detection chip (10), and the other path is connected to a first peristaltic pump (60A). A three-way solenoid valve (70) is connected to the first peristaltic pump (60A), and the two inlets of the three-way solenoid valve (70) are respectively connected to a detection liquid container (30) and a cleaning liquid container (40); A third direct solenoid valve (80C) and a third three-way pipe (90C) are sequentially connected to the third interface (1003). One path of the third three-way pipe (90C) is sequentially connected to a second peristaltic pump (60B) and a waste liquid container (50), and the other path is connected to the second interface (1002) of the detection chip (10).

2. The four-channel chip fluid control system for stem cell detection according to claim 1, characterized in that: An inlet pressure sensor and an outlet pressure sensor are further provided on the detection chip (10), and the numerical difference between the inlet pressure sensor and the outlet pressure sensor is used to judge the blockage condition of the detection chip (10).

3. The four-channel chip fluid control system for stem cell detection according to claim 1, characterized in that: The detection liquid container (30) is filled with normal saline, PBS solution or cell culture medium, and the cleaning liquid container (40) is filled with deionized water.

4. The four-channel chip fluid control system for stem cell detection according to claim 3, wherein: A second direct solenoid valve (80B) is further connected between the second three-way pipe (90B) and the fourth interface (1004).

5. The four-channel chip fluid control system for stem cell detection according to claim 4, characterized in that: The detection liquid container (30), the three-way solenoid valve (70), the first peristaltic pump (60A), the second three-way pipe (90B), the first three-way pipe (90A), the first direct solenoid valve (80A), and the detection container (20) constitute a pipeline pre-filling module for the detection container (20), which is used to realize the liquid filling of the passage between the detection liquid container (30) and the detection container (20), and the fluid pre-filling flow rate is 0.5 to 2 mL / min.

6. The four-channel chip fluid control system for stem cell detection according to claim 5, wherein The detection liquid container (30), the three-way solenoid valve (70), the first peristaltic pump (60A), the second three-way pipe (90B), the first three-way pipe (90A), the detection chip (10), the second direct solenoid valve (80B), the third direct solenoid valve (80C), the third three-way pipe (90C), the second peristaltic pump (60B), and the waste liquid container (50) constitute a pipeline pre-filling module for the detection chip (10), which is used to realize the liquid pre-filling of the pipeline between the detection chip (10) and the waste liquid container (50), and the fluid pre-filling flow rate is 0.5 to 2 mL / min.

7. The four-channel chip fluid control system for stem cell detection according to claim 6, wherein: The detection container (20), the first direct solenoid valve (80A), the first three-way pipe (90A), the detection chip (10), the third three-way pipe (90C), the second peristaltic pump (60B), and the waste liquid container (50) constitute a stem cell detection module; The stem cell detection module includes two operating conditions. One is the rapid sample injection condition of the detection solution, which is used to achieve the rapid flow of the stem cell suspension to be detected in the detection container (20) to the detection area of the detection chip (10). At this time, the fluid flow rate is 0.5 to 2 mL / min. The other is the stem cell detection condition, which is used to achieve the uniform and sequential passage of the stem cell suspension through the detection area of the detection chip (10). At this time, the fluid flow rate is 0.1 to 1 mL / min.

8. The four-channel chip fluid control system for stem cell detection according to claim 7, characterized in that: The cleaning solution container (40), three-way solenoid valve (70), first peristaltic pump (60A), second three-way pipe (90B), first three-way pipe (90A), first direct solenoid valve (80A) and detection container (20) constitute the pipeline cleaning module of the detection container (20), which is used to realize the liquid cleaning of the passage between the cleaning solution container (40) and the detection container (20). At this time, the fluid cleaning flow rate is 0.5 to 5 mL / min.

9. The four-channel chip fluid control system for stem cell detection according to claim 8, wherein: The cleaning solution container (40), three-way solenoid valve (70), first peristaltic pump (60A), second three-way pipe (90B), first three-way pipe (90A), detection chip (10), second direct solenoid valve (80B), third direct solenoid valve (80C), third three-way pipe (90C), second peristaltic pump (60B) and waste liquid container (50) constitute the pipeline cleaning module of the detection chip (10), which is used to realize the liquid cleaning of the pipeline between the detection chip (10) and the waste liquid container (50). At this time, the fluid cleaning flow rate is 0.5 to 5 mL / min.

10. A four-channel chip fluid control system for stem cell detection according to claim 9, characterized in that: The detection container (20), first direct solenoid valve (80A), first three-way pipe (90A), detection chip (10), second three-way pipe (90B), second direct solenoid valve (80B), third direct solenoid valve (80C), third three-way pipe (90C), second peristaltic pump (60B) and waste liquid container (50) constitute the solution discharge module, which is used to realize the discharge of the solution in the whole pipeline, and the discharge flow rate is 0.5 to 2 mL / min.

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