Fermentation equipment and method based on dinorhydrin production

Abstract

The fermentation equipment comprises a tank body, a feeding opening and an inoculation opening are formed in the upper end of the tank body, a discharging pipe is arranged at the lower end of the tank body, a mounting frame is arranged at the upper end of the tank body, and a driving motor is mounted at the upper end of the mounting frame; a power output shaft of the driving motor is connected with a hollow stirring shaft which downwards extends into the tank body, the periphery of the stirring shaft is outwards provided with a plurality of hollow stirring rods communicated with the stirring shaft, a communicating sleeve which is externally sleeved with the stirring shaft is arranged in the mounting frame, the stirring shaft is internally provided with a communicating port communicated with the stirring shaft, and the communicating sleeve is outwards communicated with a communicating pipe; the free end of the communicating pipe is communicated with a heating sleeve, an electric heating wire is arranged in the heating sleeve, the free end of the heating sleeve is communicated with a nitrogen access pipe and an air access pipe, and the free end of the air access pipe is communicated with an air pump. According to the invention, rapid temperature control and brand-new dissolved oxygen control can be realized, so that the production efficiency and the product quality of dinorhydrin are improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of product fermentation, and relates to a fermentation device and method based on the production of dihydromyricetin. Background Art

[0002] With the development of biotechnology and fermentation engineering technologies, using microbial fermentation to produce natural products with high added value has become one of the research hotspots. Dihydromyricetin (DHM), as an important flavonoid compound, shows broad application prospects in the fields of medicine, health food, etc. due to its significant biological activities such as antioxidant and anti-inflammatory effects. Traditional methods for extracting dihydromyricetin mainly rely on plant extraction. However, this method is limited by the seasonality and regionality of natural resources and is difficult to meet the needs of large-scale industrial production. Therefore, producing dihydromyricetin through the microbial fermentation pathway has become a very promising alternative solution.

[0003] During the dihydromyricetin fermentation process, in order to ensure that microorganisms efficiently convert substrates and synthesize target products, precisely controlling the fermentation conditions is particularly important. In particular, the regulation of temperature and dissolved oxygen is crucial for maintaining the optimal growth state of microorganisms and promoting the synthesis of metabolites. An ideal fermentation environment requires the ability to achieve rapid and precise temperature regulation to adapt to the needs of different stages in the microbial growth cycle. At the same time, a sufficient dissolved oxygen level is one of the key factors to ensure the respiration and metabolic activities of microorganisms.

[0004] However, existing fermentation equipment has certain limitations in these aspects. Traditional fermentation tanks usually adjust the temperature by slowly transferring heat from the outside to the inside. This method has a slow response speed and is difficult to meet the rapid requirements for temperature changes during the fermentation process. In addition, increasing the dissolved oxygen in the fermentation broth is mainly achieved by increasing the stirring speed. However, too high a stirring speed may cause mechanical damage to microorganisms, affecting their growth and metabolic efficiency, thereby reducing the yield and quality of the final product. Summary of the Invention

[0005] The purpose of the present invention is to provide a fermentation device and method based on the production of dihydromyricetin, which can achieve rapid temperature control and a new dissolved oxygen control to improve the production efficiency and product quality of dihydromyricetin.

[0006] To solve the above technical problems, the present invention provides a fermentation device based on the production of dihydrolinalool, including a tank body. A feeding port and an inoculation port are provided at the upper end of the tank body. The feeding port is detachably connected with a sealing cover, and the inoculation port is detachably connected with a sealing plug. A discharge pipe is provided at the lower end of the tank body. An installation frame is provided at the upper end of the tank body. A driving motor is installed at the upper end of the installation frame. The power output shaft of the driving motor is connected with a hollow stirring shaft that extends downward into the tank body. A plurality of hollow stirring rods communicating with the stirring shaft are arranged outward on the outer periphery of the stirring shaft. A communicating sleeve sleeving the stirring shaft is arranged in the installation frame. A communicating port communicating with the communicating sleeve is opened in the stirring shaft within the communicating sleeve. The communicating sleeve communicates outward with a communicating pipe. The free end of the communicating pipe communicates with a heating sleeve. An electric heating wire is arranged in the heating sleeve. The free end of the heating sleeve communicates with a nitrogen access pipe and an air access pipe. Solenoid valves are arranged on both the nitrogen access pipe and the air access pipe. The free end of the air access pipe communicates with an air pump;

[0007] A sealing cover is detachably connected to the lower end of the discharge pipe. A plurality of filter holes are opened in the side wall of the discharge pipe. A sealed drainage cylinder is sleeved on the outer periphery of the discharge pipe. A filter cloth is arranged on the outer periphery of the discharge pipe within the sealed drainage cylinder. The lower end of the sealed drainage cylinder communicates outward with a liquid discharge pipe. A valve is arranged on the liquid discharge pipe. The lower end of the stirring shaft is connected with a spiral extrusion conveying rod extending into the discharge pipe. The pitch of the spiral blades on the spiral extrusion conveying rod gradually decreases from top to bottom.

[0008] The present invention is further configured such that a pressure gauge for detecting the internal air pressure of the tank body is provided at the upper end of the tank body, a temperature sensor for detecting the internal temperature of the tank body is provided at the upper end of the tank body, a pH sensor for detecting the internal pH value of the tank body is provided at the upper end of the tank body, and a dissolved oxygen sensor for detecting the internal dissolved oxygen concentration of the tank body is provided at the upper end of the tank body.

[0009] The present invention is further configured such that an exhaust pipe and a safety valve are communicated with the upper end of the tank body. A valve is arranged on the exhaust pipe.

[0010] The present invention is further configured such that a liquid adding pipe is communicated with the upper end of the tank body. A valve is arranged on the liquid adding pipe.

[0011] The present invention is further configured such that a heat preservation sleeve is arranged on the outer side of the tank body, and a gap is left between the inner wall of the heat preservation sleeve and the outer wall of the tank body.

[0012] The present invention is further configured such that a vertically arranged strip-shaped transparent observation port is arranged on the outer side of the tank body, and the strip-shaped transparent observation port extends out of the heat preservation sleeve.

[0013] The present invention is further configured such that the connecting pipe is further connected to a water receiving pipe, and a solenoid valve is provided on the water receiving pipe.

[0014] The present invention is further configured such that a sterilizing pipe communicating therewith is provided in the middle of the air access pipe, and an ultraviolet disinfection lamp tube is provided in the sterilizing pipe along its length direction.

[0015] The present invention is further configured such that each hollow stirring rod is spirally arranged downward and outward, and a spray head communicating therewith is provided at the free end of each hollow stirring rod.

[0016] The present invention also discloses a method for double-drop alcohol fermentation, comprising the following steps:

[0017] S1. Prepare the fermentation raw materials: 40 - 50 g / L of glucose, 5 - 8 g / L of phytosterol, 4 - 6 g / L of ammonium sulfate, 2 - 3 g / L of potassium dihydrogen phosphate, 0.5 - 1.0 g / L of magnesium sulfate, 5 - 8 g / L of yeast powder;

[0018] S2. Pass high-temperature steam at 115°C - 125°C into the tank for sterilization for 20 min;

[0019] S3. Add the fermentation raw materials in step S1 into the tube body from the feeding port;

[0020] S4. Open the inoculation port and inoculate mycobacteria from the inoculation port;

[0021] S6. Temperature control: First, introduce nitrogen through the nitrogen access pipe. The nitrogen is heated by the electric heating wire in the heating sleeve, then enters the stirring shaft through the connecting pipe, and then is sprayed into the fermentation raw materials through the hollow stirring rod. And the driving motor drives the hollow stirring rod to rotate continuously, so that the heated nitrogen rapidly heats the fermentation raw materials. Among them, the temperature is controlled at 30°C - 32°C in the first 48 h, and the temperature is controlled at 28°C - 30°C in the subsequent 48 - 120 h;

[0022] S7. pH control: Automatically add 5% NaOH into the tank to maintain the pH at 4.5 - 5.0;

[0023] S8. Dissolved oxygen control: After step S6, stop introducing nitrogen, start the air pump, introduce air through the air access pipe. The air is heated by the electric heating wire in the heating sleeve, then enters the stirring shaft through the connecting pipe, and then is sprayed into the fermentation raw materials through the hollow stirring rod. If the dissolved oxygen content is too high, introduce nitrogen, if the dissolved oxygen content is too low, introduce air. The dissolved oxygen content is controlled at 20% - 30% in the first 48 h, and the dissolved oxygen content is maintained at 10% - 20%;

[0024] S9. When the conversion rate of phytosterol ≥ 85% and the concentration of dihydrolophine ≥ 6 g / L, the fermentation is terminated.

[0025] S10. After the fermentation is terminated, the driving motor drives the stirring shaft to rotate reversely, driving the spiral extrusion conveying rod to push the product downward through the discharge pipe. The liquid part inside continuously flows out through the filter holes and the filter cloth. The spiral extrusion conveying rod rotates downward, squeezing the product through the spiral blades with gradually decreasing pitch, gradually squeezing out the liquid part in the product. Finally, the dried solid product is discharged from the lower end of the discharge pipe, and the squeezed liquid part is discharged through the drain pipe in the sealed drainage cylinder.

[0026] S11. The solid product is extracted with methanol to obtain the crude extract of dihydrolophine.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] First, the present invention uses the method of continuously introducing heated nitrogen or air into the tank through the rotating hollow stirring rod to quickly heat up the raw materials. During the process, the raw materials are continuously stirred to achieve rapid and uniform heating of the raw materials. Compared with the traditional heating method from the outside to the inside, the method from the inside to the outside in the present invention has higher efficiency and better effect.

[0029] Second, when controlling the dissolved oxygen content in the present invention, air is directly introduced into the interior through the central control stirring rod, rather than increasing the oxygen content by the traditional method of high-speed stirring. Only a lower stirring speed is required to evenly mix the air, which will not cause mechanical damage to the microorganisms. And if the dissolved oxygen content is too high, the dissolved oxygen content can be gradually reduced by introducing nitrogen into the interior to prevent side reactions caused by too high dissolved oxygen content.

[0030] Third, the fermentation equipment of the present invention also has the function of solid-liquid separation. At the outlet position, the generated product is extruded and filtered by the spiral extrusion method, and there is no need to transfer it to the filtering equipment additionally for solid-liquid separation, which can effectively simplify the preparation steps and reduce the equipment cost. Description of the Drawings

[0031] Figure 1 is the overall structural schematic diagram of the present invention;

[0032] Figure 2 is the partial cross-sectional view for showing the internal structure of the tank;

[0033] Figure 3 is used to show the internal structure of the heating sleeve and the sterilization tube;

[0034] Figure 4 is used to show the connection of the stirring shaft with the hollow stirring rod and the spiral extrusion conveying rod;

[0035] Figure 5 It is a partial cross-sectional view for showing the internal structure of the discharge pipe.

[0036] Among them, 1. tank body; 2. support leg; 3. heat preservation sleeve; 4. strip-shaped transparent observation port; 5. feeding port; 6. inoculation port; 7. sealing cover; 8. sealing plug; 9. discharge pipe; 10. pressure gauge; 11. temperature sensor; 12. acid-base sensor; 13. dissolved oxygen sensor; 14. exhaust pipe; 15. safety valve; 16. valve; 17. liquid adding pipe; 18. mounting rack; 19. driving motor; 20. stirring shaft; 21. hollow stirring rod; 22. spray head; 23. connecting sleeve; 24. connecting port; 25. connecting pipe; 26. heating sleeve; 27. electric heating wire; 28. nitrogen access pipe; 29. air access pipe; 30. air pump; 31. solenoid valve; 32. sterilizing pipe; 33. ultraviolet disinfection lamp tube; 34. water connection pipe; 35. sealing cover; 36. filter hole; 37. sealed drainage cylinder; 38. filter cloth; 39. liquid discharge pipe; 40. spiral extrusion conveying rod. Specific embodiments

[0037] The following further elaborates in detail on the fermentation equipment and method based on dihydroxy alcohol production proposed by the present invention in conjunction with the accompanying drawings and specific embodiments. According to the following description, the advantages and features of the present invention will be clearer. It should be noted that the accompanying drawings are all in a very simplified form and use non-precise scales, only for conveniently and clearly assisting in explaining the purpose of the embodiments of the present invention. The same or similar reference numerals in the drawings represent the same or similar components.

[0038] Example 1, referring to Figures 1-5, A fermentation device based on the production of dihydrolinalool, including a tank body 1. Three legs 2 are arranged downward at the lower end of the tank body 1. A layer of heat preservation sleeve 3 is arranged outside the tank body 1. There is a gap between the inner wall of the heat preservation sleeve 3 and the outer wall of the tank body 1. A vertically arranged strip-shaped transparent observation port 4 is arranged outside the tank body 1, and the strip-shaped transparent observation port 4 extends out of the heat preservation sleeve 3, facilitating observing the internal situation of the tank body 1 through the strip-shaped transparent observation port 4. A feeding port 5 and an inoculation port 6 are arranged at the upper end of the tank body 1. A sealing cover 7 is detachably connected to the feeding port 5, and a sealing plug 8 is detachably connected to the inoculation port 6. A discharge pipe 9 is arranged at the lower end of the tank body 1. A pressure gauge 10 for detecting the internal air pressure is arranged at the upper end of the tank body 1. A temperature sensor 11 for detecting the internal temperature is arranged at the upper end of the tank body 1. An acid-base sensor 12 for detecting the internal acidity and alkalinity is arranged at the upper end of the tank body 1. A dissolved oxygen sensor 13 for detecting the internal dissolved oxygen concentration is arranged at the upper end of the tank body 1. An exhaust pipe 14 and a safety valve 15 are connected to the upper end of the tank body 1. A valve 16 is arranged on the exhaust pipe 14. A liquid adding pipe 17 is connected to the upper end of the tank body 1, and a valve 16 is arranged on the liquid adding pipe 17, facilitating the supplement of raw materials during the fermentation process.

[0039] An installation frame 18 is arranged at the upper end of the tank body 1. A driving motor 19 is installed at the upper end of the installation frame 18. The power output shaft of the driving motor 19 is connected with a hollow stirring shaft 20 that extends downward into the tank body 1. Five hollow stirring rods 21 communicating with it are arranged outward on the outer circumference of the stirring shaft 20. The hollow stirring rods 21 are staggered from top to bottom. Each hollow stirring rod 21 is spirally arranged downward and outward. A nozzle 22 communicating with it is arranged at the free end of each hollow stirring rod 21, facilitating spraying water or gas outward when the central control stirring rod rotates. A connecting sleeve 23 that sleeves the stirring shaft 20 and is hermetically rotatably connected to it is arranged inside the installation frame 18. The inner diameter of the connecting sleeve 23 is larger than the outer diameter of the stirring shaft 20. Two connecting ports 24 communicating with it are arranged on the stirring shaft 20 inside the connecting sleeve 23. A connecting pipe 25 communicates outward from the connecting sleeve 23. The free end of the connecting pipe 25 communicates with a heating sleeve 26. An electric heating wire 27 is arranged inside the heating sleeve 26. The free end of the heating sleeve 26 communicates with a nitrogen access pipe 28 and an air access pipe 29. The nitrogen access pipe 28 is directly connected to a compressed nitrogen tank. The free end of the air access pipe 29 communicates with an air pump 30. Solenoid valves 31 are arranged on both the nitrogen access pipe 28 and the air access pipe 29. A sterilization pipe 32 communicating with it is arranged in the middle of the air access pipe 29. An ultraviolet disinfection lamp tube 33 arranged along its length direction is arranged inside the sterilization pipe 32, making the accessed air sterile air. The connecting pipe 25 also communicates with a water receiving pipe 34. A solenoid valve 31 is arranged on the water receiving pipe 34, facilitating adding water or high-temperature steam into the tank body 1 during cleaning or disinfection.

[0040] A sealing cover 35 is detachably connected to the lower end of the discharge pipe 9. A number of filter holes 36 are provided on the side wall of the discharge pipe 9. A sealed drainage cylinder 37 is sleeved outside the outer periphery of the discharge pipe 9. A layer of filter cloth 38 is arranged inside the sealed drainage cylinder 37 on the outer periphery of the discharge pipe 9. A liquid discharge pipe 39 communicates outward from the lower end of the sealed drainage cylinder 37. A valve 16 is arranged on the liquid discharge pipe 39. The lower end of the stirring shaft 20 is connected with a spiral extrusion conveying rod 40 extending into the discharge pipe 9. The pitch of the spiral blades on the spiral extrusion conveying rod 40 gradually becomes smaller from top to bottom. During the fermentation process, the driving motor 19 drives the stirring shaft 20 to rotate forward, so that the spiral extrusion conveying rod 40 rotates upward, and the materials in the discharge pipe 9 can be continuously exported upward, preventing incomplete fermentation at the bottom of the discharge pipe 9. When discharging, the driving motor 19 drives the stirring shaft 20 to rotate reversely, and the spiral extrusion conveying rod 40 rotates downward, so that the solid-liquid mixture is discharged downward. During the downward movement of the solid-liquid mixture along with the spiral extrusion conveying rod 40, since the distance between the spiral blades on both sides of the mixture continuously becomes smaller downward, the liquid in the mixture is extruded, and it enters the sealed drainage cylinder 37 through the filter cloth 38, while the solid product obtained after pressure filtration is discharged from the lower end of the discharge pipe 9.

[0041] Example 2, a method for double-nordihydroguaiaretic acid fermentation, uses the fermentation equipment based on the production of double-nordihydroguaiaretic acid in Example 1, and includes the following steps:

[0042] S1. Prepare the fermentation raw materials: 40 - 50 g / L of glucose, 5 - 8 g / L of phytosterol, 4 - 6 g / L of ammonium sulfate, 2 - 3 g / L of potassium dihydrogen phosphate, 0.5 - 1.0 g / L of magnesium sulfate, 5 - 8 g / L of yeast powder;

[0043] S2. Introduce high-temperature steam at 115°C - 125°C into the tank body 1 for sterilization for 20 min;

[0044] S3. Add the fermentation raw materials in step S1 into the pipe body from the feeding port 5;

[0045] S4. Open the inoculation port 6 and inoculate mycobacteria from the inoculation port 6;

[0046] S6. Temperature control: First, introduce nitrogen through the nitrogen access pipe 28. The nitrogen is heated by the electric heating wire 27 in the heating sleeve 26, and then enters the stirring shaft 20 through the connecting pipe 25, and then is sprayed into the fermentation raw materials through the hollow stirring rod 21. And the driving motor 19 drives the hollow stirring rod 21 to rotate continuously, so that the heated nitrogen rapidly heats the fermentation raw materials. Among them, the temperature is controlled at 30°C - 32°C in the first 48 h, and the temperature is controlled at 28°C - 30°C in the subsequent 48 - 120 h;

[0047] S7, pH control: Automatically add 5% NaOH into the tank body 1 to maintain the pH at 4.5 - 5.0;

[0048] S8, dissolved oxygen control: After step S6, stop introducing nitrogen, start the air pump 30, introduce air through the air access pipe 29. The air is heated by the electric heating wire 27 in the heating sleeve 26, and then enters the stirring shaft 20 through the connecting pipe 25, and then is sprayed into the fermentation raw materials through the hollow stirring rod 21. If the dissolved oxygen content is too high, introduce nitrogen; if the dissolved oxygen content is too low, introduce air. The dissolved oxygen content is controlled at 20% - 30% in the first 48 hours, and the dissolved oxygen content is maintained at 10% - 20%;

[0049] S9, When the conversion rate of phytosterol ≥ 85% and the concentration of dihydrolophine ≥ 6 g / L, the fermentation terminates;

[0050] S10, After the fermentation terminates, the drive motor 19 drives the stirring shaft 20 to rotate in reverse, driving the spiral extrusion conveying rod 40 to push the product downward through the discharge pipe 9. The liquid part inside continuously flows out through the filter holes 36 and the filter cloth 38. The spiral extrusion conveying rod 40 rotates downward, and the product is extruded by the spiral blades with continuously decreasing pitches on it, gradually squeezing out the liquid part in the product. Finally, the dried solid product is discharged from the lower end of the discharge pipe 9, and the squeezed liquid part is discharged through the drain pipe in the sealed drainage cylinder 37;

[0051] S11, Extract the solid product with methanol to obtain the crude extract of dihydrolophine.

[0052] It should also be supplemented and explained that all "settings" and similar descriptive words in this application (especially in the specification) express that there is a connection relationship between two structures, but the specific means of connection between the two are not limited too much, and usually are conventional connection means, that is, it should be understood that this means is the prior art and does not need to be elaborated too much. For example, "n is provided on m" only expresses that there is an n structure on the m structure, and whether the two are connected by welding, riveting, adhesive connection or integrally formed is within the protection scope of this application; another example, "y is rotatably provided on x" only expresses that y and x can rotate relative to each other, and as for whether the two are rotatably connected by a bearing, or y directly passes through x and is rotatably connected to x, or other achievable ways, they are all within the protection scope of this application.

[0053] The above description is only a description of the preferred embodiments of the present invention, and does not limit the scope of the present invention in any way. Any changes and modifications made by those of ordinary skill in the art of the present invention based on the above disclosure are within the protection scope of the claims.

Claims

1. A fermentation device based on the production of dihydrolinalool, comprising a tank body (1), wherein a feeding port (5) and an inoculation port (6) are arranged at the upper end of the tank body, the feeding port is detachably connected with a sealing cover (7), the inoculation port is detachably connected with a sealing plug (8), and a discharge pipe (9) is arranged at the lower end of the tank body, characterized in that, An installation frame (18) is provided at the upper end of the tank body. A driving motor (19) is installed at the upper end of the installation frame. The power output shaft of the driving motor is connected to a hollow stirring shaft (20) that extends downward into the tank body. A plurality of hollow stirring rods (21) communicating with the stirring shaft are arranged outward on the outer periphery of the stirring shaft. A communicating sleeve (23) sleeving the stirring shaft is arranged in the installation frame. A communicating port (24) communicating with the communicating sleeve is formed in the stirring shaft. The communicating sleeve communicates outward with a communicating pipe (25). The free end of the communicating pipe communicates with a heating sleeve (26). An electric heating wire (27) is arranged in the heating sleeve. The free end of the heating sleeve communicates with a nitrogen gas access pipe (28) and an air access pipe (29). Solenoid valves (31) are arranged on both the nitrogen gas access pipe and the air access pipe. The free end of the air access pipe communicates with an air pump (30); A sealing cover (35) is detachably connected to the lower end of the discharge pipe. A plurality of filter holes (36) are formed in the side wall of the discharge pipe. A sealed drainage cylinder (37) is sleeved on the outer periphery of the discharge pipe. A filter cloth (38) is arranged in the sealed drainage cylinder on the outer periphery of the discharge pipe. The lower end of the sealed drainage cylinder communicates outward with a liquid discharge pipe (39). A valve (16) is arranged on the liquid discharge pipe. The lower end of the stirring shaft is connected to a spiral extrusion conveying rod (40) extending into the discharge pipe. The pitch of the spiral blades on the spiral extrusion conveying rod gradually decreases from top to bottom.

2. The fermentation equipment based on the production of dihydrolinalool according to claim 1, characterized in that, A pressure gauge (10) for detecting the internal air pressure is provided at the upper end of the tank body. A temperature sensor (11) for detecting the internal temperature is provided at the upper end of the tank body. An acid-base sensor (12) for detecting the internal acidity and alkalinity is provided at the upper end of the tank body. A dissolved oxygen sensor (13) for detecting the internal dissolved oxygen concentration is provided at the upper end of the tank body.

3. The fermentation equipment based on the production of ditanshinol according to claim 1, wherein, An exhaust pipe (14) and a safety valve (15) are communicated with the upper end of the tank body. A valve is arranged on the exhaust pipe.

4. The fermentation equipment based on the production of bicyclohexanol according to claim 1, characterized in that, A liquid adding pipe (17) is communicated with the upper end of the tank body. A valve is arranged on the liquid adding pipe.

5. The fermentation equipment based on the production of dihydrolinalool according to claim 1, characterized in that, A heat preservation sleeve (3) is arranged on the outer side of the tank body. A gap is left between the inner wall of the heat preservation sleeve and the outer wall of the tank body.

6. The fermentation equipment based on the production of ditanshinol according to claim 5, wherein, A vertically arranged strip-shaped transparent observation port (4) is arranged on the outer side of the tank body. The strip-shaped transparent observation port extends out of the heat preservation sleeve.

7. The fermentation equipment based on the production of dihydrolinalool according to claim 1, characterized in that, The communicating pipe also communicates with a water receiving pipe (34). A solenoid valve is arranged on the water receiving pipe.

8. The fermentation equipment based on the production of bicyclohexanol according to claim 1, wherein, A sterilizing pipe (32) communicating with the air access pipe is arranged in the middle of the air access pipe. An ultraviolet disinfection lamp tube (33) arranged along the length direction of the sterilizing pipe is arranged in the sterilizing pipe.

9. The fermentation equipment based on the production of dihydromyricetin according to claim 1, characterized in that, Each hollow stirring rod is spirally arranged downward and outward. A nozzle (22) communicating with each hollow stirring rod is arranged at the free end of each hollow stirring rod.

10. A method for fermenting dihydrolinalool, using the fermentation equipment based on the production of dihydrolinalool described in any one of claims 1-9, characterized in that, Including the following steps: S1. Prepare the fermentation raw materials: 40 - 50 g / L of glucose, 5 - 8 g / L of phytosterol, 4 - 6 g / L of ammonium sulfate, 2 - 3 g / L of potassium dihydrogen phosphate, 0.5 - 1.0 g / L of magnesium sulfate, 5 - 8 g / L of yeast powder; S2. Introduce high-temperature steam at 115°C - 125°C into the tank and sterilize for 20 min; S3. Add the fermentation raw material in step S1 into the tube from the feeding port; S4. Open the inoculation port and inoculate mycobacterium from the inoculation port; S6. Temperature control: First, introduce nitrogen through the nitrogen access tube. The nitrogen is heated by the electric heating wire in the heating sleeve, then enters the stirring shaft through the connecting tube, and then is sprayed into the fermentation raw material through the hollow stirring rod. And the driving motor drives the hollow stirring rod to rotate continuously, so that the heated nitrogen rapidly heats the fermentation raw material. Among them, the temperature is controlled at 30°C - 32°C in the first 48 h, and the temperature is controlled at 28°C - 30°C in the subsequent 48 - 120 h; S7. pH control: Automatically add 5% NaOH to the tank to maintain the pH at 4.5 - 5.0; S8. Dissolved oxygen control: After step S6, stop introducing nitrogen, start the air pump, introduce air through the air access tube. The air is heated by the electric heating wire in the heating sleeve, then enters the stirring shaft through the connecting tube, and then is sprayed into the fermentation raw material through the hollow stirring rod. If the dissolved oxygen content is too high, introduce nitrogen, and if the dissolved oxygen content is too low, introduce air. The dissolved oxygen content is controlled at 20% - 30% in the first 48 h, and the dissolved oxygen content is maintained at 10% - 20%; S9. When the conversion rate of phytosterol ≥ 85% and the concentration of dihydrolophosterol ≥ 6 g / L, the fermentation terminates; S10. After the fermentation terminates, the driving motor drives the stirring shaft to rotate in reverse, driving the spiral extrusion conveyor rod to push the product downward through the discharge pipe. The liquid part inside continuously flows out through the filter holes and the filter cloth. The spiral extrusion conveyor rod rotates downward, and the product is extruded by the spiral blades with continuously decreasing pitches on it, gradually squeezing out the liquid part in the product. Finally, the dried solid product is discharged from the lower end of the discharge pipe, and the squeezed liquid part is discharged through the drain pipe in the sealed drainage cylinder; S11. Extract the solid product with methanol to obtain the crude extract of dihydrolophosterol.

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