Organic polymer filler surface modification method for accelerating biofilm formation

Abstract

The invention provides an organic polymer filler surface modification method for accelerating biofilm formation, and belongs to the technical field of environmental treatment. The preparation method comprises the following steps: soaking an organic polymer filler with a gram-negative bacterium solution, taking out the organic polymer filler, continuously soaking the organic polymer filler with a mixed solution of a nutrient reagent, carrageenan and a curing agent, and cleaning and drying the organic polymer filler after soaking to obtain the modified organic polymer filler. According to the method, the adhesion strength of the biological membrane of the organic polymer filler can be promoted, the formation of the biological membrane and the long-term stable operation of a biological membrane reactor are facilitated, the formation efficiency of the biological membrane is improved, and the method has the advantages of being simple and convenient to operate, low in cost and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of environmental treatment, and particularly relates to a method for surface modification of an organic polymer filler for accelerating biofilm formation. Background Art

[0002] With the development of social industry and the increase in consumption of traditional fossil fuels, a large amount of pollutants are generated and discharged into the natural environment. The damage of these pollutants to public health is becoming more and more serious, and environmental pollution control has become a global cause to ensure the living standards of human beings and the sustainable development of society. At present, there are many technologies and processes for treating various forms of pollutants. Among them, the biofilm treatment method has been gradually concerned because of its advantages such as high cost performance, low secondary pollution, stable operation, and simple operation. Currently, the more commonly used biofilm reactors include biological filters, biological rotating discs, biological contact oxidation equipment, and biological fluidized beds used for wastewater treatment, as well as biological filtration towers and biological trickling filters used for waste gas treatment.

[0003] As the main component in the biofilm reactor, the filler not only provides a growth environment for microorganisms, but also has an important impact on the mass transfer between the gas phase, liquid phase, and biofilm phase in the reactor. Therefore, the physicochemical properties of the filler in the biofilm reactor are of great significance for the formation of biofilms, and the formation of biofilms directly affects the operation performance of the biofilm reactor. Currently, there are many types of biofilm fillers in application. Compared with other fillers, organic polymer fillers have the advantages of easy availability, low cost, long service life, and high resilience rate. They are widely used as fillers in the process of treating pollutants by biofilm reactors. However, the process of microorganisms forming biofilms on the surface of organic polymer fillers involves complex regulatory mechanisms and is affected by various environmental factors, often resulting in low biofilm formation efficiency, which in turn prolongs the start-up time of the biofilm reactor and reduces its operation performance. Summary of the Invention

[0004] To solve the above technical problems, the present invention proposes a method for surface modification of an organic polymer filler for accelerating biofilm formation. By immersing the filler in a Gram-negative bacteria solution and using a fixing agent and a nutrient reagent, the purpose of surface modification is achieved, so that the organic polymer filler can release AHLs (N-acyl homoserine lactones), further promoting the production of EPS (extracellular polymeric substances) and the aggregation of microorganisms on the filler, which is beneficial to the formation of biofilms and the long-term stable operation of the reactor.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] One of the technical solutions of the present invention:

[0007] A method for surface modification of organic polymer fillers to accelerate biofilm formation, which involves soaking the organic polymer fillers with a Gram-negative bacteria solution, taking them out and then soaking them with a mixed solution of a nutrient reagent and carrageenan, and after the soaking is completed, cleaning and drying the organic polymer fillers to obtain surface-modified organic polymer fillers.

[0008] Furthermore, the soaking time of the organic polymer fillers with the Gram-negative bacteria solution is greater than 12 hours.

[0009] Even further, the Gram-negative bacteria solution is obtained by inoculating a Gram-negative bacteria strain that can secrete AHLs into an LB liquid medium and culturing it in a shaker at 30°C and 180 r / min until OD 600 ≈0.8, and the Gram-negative bacteria strain that can secrete AHLs used can be isolated and screened from activated sludge.

[0010] Furthermore, the nutrient reagent is prepared by dissolving sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, trace elements, and vitamins in water.

[0011] Even further, in the nutrient reagent, the concentration of sodium acetate is (24 - 36) g / L, the concentration of ammonium sulfate is 2.4 g / L, the concentration of potassium dihydrogen phosphate is 1 g / L, the concentration of trace elements is 100 μL / L, and the concentration of vitamins is 100 μL / L. More specifically, the preparation method of the nutrient reagent is carried out according to the following steps: adding 24 - 36 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace elements, and 100 μL of vitamins to 1 L of water, and stirring evenly to obtain.

[0012] Furthermore, the dosage of carrageenan in the mixed solution is 10 g / L of the mass of the nutrient reagent.

[0013] Furthermore, the mixed solution also contains a curing agent (potassium chloride or calcium chloride), and the dosage of this curing agent is 0.1% - 0.3% of the total mass of the gel formed by carrageenan and the nutrient reagent.

[0014] Even further, the curing agent is potassium chloride or calcium chloride, and the purpose of adding the curing agent is to interact with carrageenan molecules to improve the strength of the carrageenan gel.

[0015] Furthermore, the organic polymer fillers are selected from one or more of polyurethane, polyethylene, and polyvinyl chloride.

[0016] The present invention realizes the functional design of organic polymer fillers through an innovative bio-physical composite modification technology:

[0017] (1) First, load the bacterial solution of Gram-negative bacteria secreting AHLs into the immersion tank. Utilize the microfluidic impact and local high-temperature and high-pressure environment generated by the ultrasonic cavitation effect to destroy the surface hydrophobic structure of the organic polymer filler (polyurethane / polyethylene / polyvinyl chloride) and form nano-scale micropores. Achieve the uniform anchoring of the strains through the hydrogen bond interaction between the lipopolysaccharide on the strain surface and the polar groups (-OH / -COOH) on the filler surface. This biological modification process forms an initial biofilm precursor layer through the interfacial bridging effect of microbial extracellular polysaccharides (such as alginate);

[0018] (2) Then, construct a carrageenan-potassium chloride / calcium chloride ion-crosslinked gel system as the functional carrier: Adopt a thermoreversible gelation process (dissolve carrageenan at 60 - 70 °C and gelate at 35 - 45 °C). Utilize the sulfate ester groups on the carrageenan molecular chain to form an egg-box structure with divalent cations (Ca 2+ ), form a three-dimensional network gel on the filler surface. Nutritional reagents (containing sodium acetate / ammonium sulfate / phosphate, etc.) provide the essential carbon, nitrogen, and phosphorus sources for microbial growth. Trace elements, etc. activate the quorum sensing signal synthesis enzyme, and vitamins promote the metabolic activity of the strains. This system realizes the sustained release of AHLs through a diffusion-controlled release mechanism;

[0019] After modification in the present invention, a triple-functional interface is formed on the filler surface: ① Microbial layer: The immobilized strains continuously grow in the gel microenvironment and secrete AHLs through the quorum sensing mechanism; ② Gel layer: The carrageenan network provides a biocompatible microenvironment and simultaneously serves as a slow-release matrix for AHLs; ③ Filler layer: The microporous structure provides mechanical support. As a quorum sensing signal molecule, AHLs activate the expression of the EPS operon through signal transduction mediated by the LuxR-type receptor protein, promoting the secretion of extracellular polysaccharides and proteins (such as forming a three-dimensional network structure of EPS. EPS significantly enhances biofilm formation through the bridging of multivalent cations (Ca 2+ ) and the mechanical interlocking of the fimbriae on the microbial cell surface.

[0020] Compared with the prior art, the present invention has the following advantages and technical effects:

[0021] In the present invention, AHLs-secreting bacteria are attached to the surface of the organic polymer filler, and carrageenan and nutritional reagents are further used to immobilize the strains on its surface, thereby achieving the modification purpose. The present invention can promote the formation of biofilms on the surface of organic polymer fillers and has the advantages of simple operation method and low cost. Brief Description of the Drawings

[0022] The drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation to the present invention. In the drawings:

[0023] Figure 1SEM image of unmodified polyurethane filler;

[0024] Figure 2 SEM image of surface-modified organic polymer filler prepared by modification in Example 1;

[0025] Note: 1 cm on the figure represents 0.15 mm in reality. Detailed implementation manners

[0026] Now, various exemplary implementation manners of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.

[0027] It should be understood that the terms used in the present invention are only for describing specific implementation manners and are not used to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0028] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0029] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific implementation manners of the present invention specification, which are obvious to those skilled in the art. Other implementation manners obtained from the present invention specification are obvious to those skilled in the art. The present invention specification and examples are only exemplary.

[0030] Regarding the terms "comprising", "including", "having", "containing", etc. used herein, they are all open-ended terms, meaning including but not limited to.

[0031] An embodiment of the present invention provides a method for surface modification of an organic polymer filler to accelerate biofilm formation. The organic polymer filler is soaked in a Gram-negative bacteria solution, and after taking it out, it is continuously soaked in a mixed solution of a nutrient reagent, carrageenan, and a curing agent. After the soaking is completed, the organic polymer filler is washed and dried to obtain a surface-modified organic polymer filler.

[0032] In a preferred embodiment of the present invention, the soaking time of the organic polymer filler with the Gram-negative bacteria liquid is greater than 12 hours. As an optimized technical solution, the soaking of the organic polymer filler with the Gram-negative bacteria liquid can be carried out in an ultrasonic environment. The rapid oscillation of the liquid by ultrasonic waves enables the bacteria liquid to uniformly impact the surface of the filler, so that microorganisms are uniformly attached to the surface of the filler.

[0033] In a preferred embodiment of the present invention, the Gram-negative bacteria liquid is obtained by inoculating the Gram-negative bacteria strain that can secrete AHLs into the LB liquid medium and culturing it in a shaker at 30 °C and 180 r / min until OD 600 ≈0.8, wherein the Gram-negative bacteria strain that can secrete AHLs used can be isolated and screened from activated sludge.

[0034] More specifically, the Gram-negative bacteria liquid that can secrete AHLs used in the embodiment of the present invention is prepared according to the following steps:

[0035] I. Sample collection

[0036] (1) Dip the cultured colony of the quorum sensing reporter bacterium A136 (purchased from Hangzhou Hongsai Biotechnology Co., Ltd.) with a swab and add it to the LB liquid medium, and place it in an incubator at a set temperature of 37 °C for 24 h, then store it in a 4 °C refrigerator for later use.

[0037] (2) Take 1 mL of activated sludge liquid and add it to 29 mL of sterile water, shake it to make the concentration evenly diluted. To prevent the initial concentration from being too high so as to completely cover the solid medium, take 1 mL of the diluted liquid and add it to 29 mL of sterile water for further dilution, shake it, and repeat the dilution two more times. A total of 4 portions of diluted liquid are obtained. Take 1 mL of each of the last two portions of the diluted liquid with the lowest concentration and evenly spread them on the solid medium, and place it in an incubator at a set temperature of 37 °C for 24 h. To ensure the integrity of the experimental data, two groups of parallel tests are carried out. At this time, we have completed the collection of 6 groups of samples.

[0038] (3) Observe the growth on the 6 solid media. A total of 39 independent colonies grow on the 6 media.

[0039] (4) Use an inoculation rod to dip different colonies and add them to the liquid medium to obtain 39 portions of colony samples. The culture and storage conditions are the same as those of the A136 colony.

[0040] Note: The above operations are all completed in a laminar flow hood.

[0041] II. Strain screening method

[0042] (1) Use a marker pen to evenly divide the medium area on the back of the solid medium into 39 small squares and number them. Then smear and cover the medium with X-gal solution. To prevent the possible small amount of organic matter in X-gal from affecting colony growth, wait until the X-gal solution on the medium has evaporated completely before proceeding to the next experiment.

[0043] (2) Add 39 colony samples to the center of 39 small squares respectively, and then add A136 bacterial solution to the 39 small squares to mix with the previously added colonies. Repeat the above experimental operations to obtain another two groups of parallel samples. Place the three media in an incubator and incubate for 24 h.

[0044] (3) Take out the three media and observe that only the No. 8 small square has a color reaction to produce blue. From this, we conclude that the strain in the No. 8 small square is the required Gram-negative bacterium secreting AHLs.

[0045] In a preferred embodiment of the present invention, the nutrient reagent is prepared by dissolving sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, trace elements, and vitamins in water. In the nutrient reagent, the concentration of sodium acetate is (24 - 36) g / L, the concentration of ammonium sulfate is 2.4 g / L, the concentration of potassium dihydrogen phosphate is 1 g / L, the concentration of trace elements is 100 μL / L, and the concentration of vitamins is 100 μL / L. More specifically, the preparation method of the nutrient reagent is carried out according to the following steps: Add 24 - 36 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace elements, and 100 μL of vitamins to 1 L of water, and stir evenly to obtain.

[0046] In a preferred embodiment of the present invention, the dosage of carrageenan in the mixture is 10 g / L of the mass of the nutrient reagent.

[0047] In a preferred embodiment of the present invention, the mixture further contains a curing agent, and the dosage of the curing agent is 0.1% - 0.3% of the total mass of the gel formed by carrageenan and the nutrient reagent. The curing agent is potassium chloride or calcium chloride.

[0048] In a preferred embodiment of the present invention, the organic polymer filler is selected from one or more of polyurethane, polyethylene, and polyvinyl chloride.

[0049] As an optimized technical solution, the surface modification method of the organic polymer filler for accelerating biofilm formation in the embodiment of the present invention is carried out in an immersion tank, and specifically includes the following steps:

[0050] Load the bacterial solution of Gram-negative bacteria that can secrete AHLs into the soaking pool, soak the organic polymer fillers (polyurethane, polyethylene, and polyvinyl chloride) in the bacterial solution, place the soaking pool in an ultrasonic environment for more than 12 hours, so that the surface of the organic polymer fillers is evenly attached with strains, and then take out the organic polymer fillers attached with strains to obtain bio-modified organic polymer fillers;

[0051] Prepare the nutrient reagent according to the following ratio: add 24 - 36 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace elements, and 100 μL of vitamins into 1 L of water, and stir evenly to obtain;

[0052] Add the nutrient reagent into the soaking pool, and add 1% (by mass) of carrageenan of the nutrient reagent, as well as a curing agent (potassium chloride or calcium chloride, and the addition amount of the curing agent is 0.1% - 0.3% of the gel mass formed by the nutrient reagent and carrageenan) to obtain a mixed solution. Heat to dissolve the carrageenan. After cooling to 35 - 45 °C, soak the obtained bio-modified organic polymer fillers in the soaking pool filled with the mixed solution. After the liquid in the soaking pool is completely cooled to form a gel to wrap the bio-modified organic polymer fillers, obtain the AHLs slow-release organic polymer fillers; this process is a physical modification process. Gram-negative bacteria that can secrete AHLs and the nutrient reagent are fixed in the carrageenan. The strains grow and release AHLs, which promotes the production of extracellular polymers and enhances the aggregation and adhesion between bacteria, further promoting the formation of biofilms.

[0053] After drying the AHLs slow-release organic polymer fillers, obtain the surface-modified organic polymer fillers.

[0054] During the soaking process, the liquid used needs to cover the organic polymer fillers, but there is no special limit on the specific dosage.

[0055] The LB liquid medium, sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, trace elements, vitamins, polyurethane, carrageenan, polyethylene, and polyvinyl chloride fillers used in the embodiments of the present invention are all commercially available. The compositions of the trace element solution and the vitamin solution used in the embodiments are respectively:

[0056] Trace element solution: 1.5 g of nitrilotriacetic acid, 3.0 g of magnesium sulfate heptahydrate, 0.5 g of manganese sulfate monohydrate, 1.0 g of sodium chloride, 0.1 g of ferrous sulfate heptahydrate, 0.1 g of cobalt chloride, 0.1 g of calcium chloride, 0.1 g of zinc sulfate heptahydrate, 0.01 g of copper sulfate pentahydrate, 0.01 g of potassium alum, 0.01 g of H3BO3, 0.01 g of sodium molybdate, and 1.0 L of distilled water;

[0057] Vitamin solution: 2.0 mg of biotin, 2.0 mg of vitamin B2, 10.0 mg of pyridoxine hydrochloride, 5.0 mg of vitamin B1, 5.0 mg of vitamin B2, 5.0 mg of nicotinic acid, 5.0 mg of D-calcium pantothenate, 0.1 mg of vitamin B12, 5.0 mg of p-aminobenzoic acid, 5.0 mg of lipoic acid, and 1.0 L of distilled water.

[0058] The technical solution of the present invention will be further described below through examples.

[0059] Example 1

[0060] Load the Gram-negative bacterial solution that can secrete AHLs into the soaking pool, soak the polyurethane filler in 50 mL of the bacterial solution, place the soaking pool in an ultrasonic environment for 18 hours to uniformly attach the strains on the surface of the polyurethane filler, and then take out the polyurethane filler with AHLs-secreting bacteria uniformly attached to the surface to obtain a bio-modified organic polymer filler;

[0061] Add 30 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace element solution, and 100 μL of vitamin solution to 1 L of distilled water, and stir evenly to obtain a nutrient reagent;

[0062] Add the nutrient reagent (1 L) to the soaking pool, add 10 g of carrageenan to form a gel (the amount of the formed gel is 1000 g, the same below), and add 3 g of calcium chloride to obtain a mixed solution. Heat to 75 °C to dissolve the carrageenan. After cooling to 45 °C, immerse the obtained bio-modified organic polymer filler in the soaking pool filled with the mixed solution. After the liquid in the soaking pool is completely cooled to form a gel wrapping the bio-modified organic polymer filler, an AHLs-sustained release organic polymer filler is obtained;

[0063] Wash and dry the AHLs-sustained release organic polymer filler with distilled water to obtain a surface-modified organic polymer filler.

[0064] Comparative Example 1

[0065] Unmodified polyurethane filler.

[0066] The SEM image of the unmodified polyurethane filler in Comparative Example 1 is shown in Figure 1 , and the SEM image of the surface-modified organic polymer filler prepared by modification in Example 1 is shown in Figure 2 . It can be seen that a stable gel is formed on the surface of the modified polyurethane filler, and the nutrient reagent and the Gram-negative bacteria secreting AHLs are fixed in the gel and continuously grow, realizing the continuous release of AHLs from the modified filler.

[0067] Performance test

[0068] The surface-modified organic polymer filler prepared in Example 1 and the unmodified polyurethane filler in Comparative Example 1 were respectively loaded into a biofilter tower, and the tower was operated to treat BTEX (benzene series) waste gas with TVOC (total volatile organic compounds) of 800 ppm. After 12 days of operation, the amount of biofilm on the filler surface and the removal rate of the filter tower were measured. The results showed that the amount of biofilm on the surface of the surface-modified organic polymer filler in Example 1 could reach 5.22×10 11 CFU / mL, and the amount of biofilm on the unmodified polyurethane filler in Comparative Example 1 increased by 2.99×10 11 CFU / mL, indicating that the microbial activity of the biofilm on the surface-modified organic polymer filler prepared in Example 1 of the present invention was significantly improved. Under the action of the biofilm, the removal rate of the BTEX waste gas by the biofilter tower filled with the surface-modified organic polymer filler prepared in Example 1 was increased by 30.2% compared with the filter tower equipped with the unmodified filler.

[0069] Example 2

[0070] The bacterial solution of Gram-negative bacteria capable of secreting AHLs was loaded into an immersion tank, and the polyethylene filler was immersed in 50 mL of the bacterial solution. The immersion tank was placed in an ultrasonic environment and immersed for 18 hours to uniformly attach the strains to the surface of the polyethylene filler. Then, the polyethylene filler with AHLs-secreting bacteria uniformly attached to its surface was taken out to obtain a bio-modified organic polymer filler;

[0071] 24 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace element solution, and 100 μL of vitamin solution were added to 1 L of distilled water, and after stirring evenly, a nutrient reagent was obtained;

[0072] The nutrient reagent (1 L) was added to the immersion tank, and 10 g of carrageenan and 3 g of calcium chloride were added to obtain a mixed solution. The carrageenan was dissolved by heating to 75 °C, and after cooling to 40 °C, the obtained bio-modified organic polymer filler was immersed in the immersion tank containing the mixed solution. After the liquid in the immersion tank was completely cooled to form a gel to wrap the bio-modified organic polymer filler, an AHLs-sustained-release organic polymer filler was obtained;

[0073] The AHLs-sustained-release organic polymer filler was washed with distilled water and dried to obtain a surface-modified organic polymer filler.

[0074] Comparative Example 2

[0075] The bacterial solution of Gram-negative bacteria capable of secreting AHLs was loaded into an immersion tank, and the polyurethane filler was immersed in 50 mL of the bacterial solution. The immersion tank was placed in an ultrasonic environment and immersed for 18 hours to uniformly attach the strains to the surface of the polyurethane filler. Then, the polyurethane filler with AHLs-secreting bacteria uniformly attached to its surface was taken out to obtain a bio-modified organic polymer filler.

[0076] Performance test

[0077] The surface-modified organic polymer filler prepared in Example 2 and the bio-modified organic polymer filler in Comparative Example 2 were respectively loaded into a biofilter tower, and the tower was operated to treat BTEX (benzene series) waste gas with TVOC (total volatile organic compounds) of 800 ppm. After 12 days of operation, the amount of biofilm on the filler surface and the removal rate of the filter tower were tested. The results showed that the amount of biofilm on the surface of the surface-modified organic polymer filler in Example 2 could reach 4.76×10 11 CFU / mL, which was 2.03 times that of the bio-modified organic polymer filler in Comparative Example 2, indicating that the microbial activity of the biofilm on the surface-modified organic polymer filler prepared in Example 2 of the present invention was significantly improved. Under the action of the biofilm, the removal rate of BTEX waste gas by the biofilter tower filled with the surface-modified organic polymer filler prepared in Example 2 was increased by about 19% compared with the filter tower equipped with the bio-modified organic polymer filler in Comparative Example 2.

[0078] Example 3

[0079] The bacterial solution of Gram-negative bacteria capable of secreting AHLs was loaded into an immersion pool, and polyvinyl chloride filler was immersed in 50 mL of the bacterial solution. The immersion pool was placed in an ultrasonic environment and immersed for 18 hours to make the surface of the polyvinyl chloride filler evenly adhere to the strains. Then, the polyvinyl chloride filler with AHLs-secreting bacteria evenly attached to its surface was taken out to obtain a bio-modified organic polymer filler;

[0080] 36 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace element solution and 100 μL of vitamin solution were added to 1 L of distilled water, and after stirring evenly, a nutrient reagent was obtained;

[0081] The nutrient reagent (1 L) was added to the immersion pool, and 10 g of carrageenan and 2.5 g of calcium chloride were added to obtain a mixed solution. The carrageenan was dissolved by heating to 75 °C. After cooling to 35 °C, the obtained bio-modified organic polymer filler was immersed in the immersion pool containing the mixed solution. After the liquid in the immersion pool was completely cooled to form a gel to wrap the bio-modified organic polymer filler, an AHLs-sustained-release organic polymer filler was obtained;

[0082] The AHLs-sustained-release organic polymer filler was washed with distilled water and dried to obtain a surface-modified organic polymer filler.

[0083] Comparative Example 3

[0084] Load the bacterial solution of Gram-negative bacteria that can secrete AHLs into the immersion tank. Immerse the polyurethane filler in 50 mL of the bacterial solution, and place the immersion tank in an ultrasonic environment for 18 hours to evenly attach the strains to the surface of the polyurethane filler. Then, take out the polyurethane filler with AHLs-secreting bacteria evenly attached to its surface to obtain a biologically modified organic polymer filler.

[0085] Add 30 g of sodium acetate, 2.4 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, 100 μL of trace element solution, and 100 μL of vitamin solution to 1 L of distilled water, and stir evenly to obtain a nutrient reagent.

[0086] Add the nutrient reagent (1 L) to the immersion tank, immerse the obtained biologically modified organic polymer filler in the immersion tank filled with the nutrient reagent for 10 minutes. After the immersion, take out the organic polymer filler, wash it with distilled water, and dry it to obtain a surface-modified organic polymer filler.

[0087] Respectively load the surface-modified organic polymer filler prepared in Example 3 and the surface-modified organic polymer filler in Comparative Example 3 into the biological filter tower, and operate to treat BTEX (benzene series) waste gas with a TVOC (total volatile organic compounds) of 800 ppm. After 12 days of operation, test the amount of biofilm on the filler surface and the removal rate of the filter tower. The results show that the amount of biofilm on the surface of the surface-modified organic polymer filler in Example 3 can reach 6.11×10 11 CFU / mL, and the amount of biofilm on the surface-modified organic polymer filler in Comparative Example 3 increased by 31.1%, indicating that the microbial activity of the biofilm on the surface-modified organic polymer filler prepared in Example 3 of the present invention was significantly improved. Under the action of the biofilm, the removal rate of BTEX waste gas by the biological filter tower loaded with the surface-modified organic polymer filler prepared in Example 3 was increased by about 21% compared with the filter tower equipped with the surface-modified organic polymer filler in Comparative Example 3.

[0088] The above is only the preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. A method for surface modification of an organic polymer filler to accelerate biofilm formation, characterized in that, Soak the organic polymer filler with the Gram-negative bacteria solution, take it out and continue to soak it with the mixed solution of nutrient reagent and carrageenan. After the soaking is completed, wash and dry the organic polymer filler to obtain the surface-modified organic polymer filler.

2. The method for surface modification of an organic polymer filler for accelerating biofilm formation according to claim 1, characterized in that, The soaking time of the organic polymer filler with the Gram-negative bacteria solution is more than 12 hours.

3. The method for surface modification of an organic polymer filler for accelerating biofilm formation according to claim 1, characterized in that, The nutrient reagent is prepared by dissolving sodium acetate, ammonium sulfate, potassium dihydrogen phosphate, trace elements and vitamins in water.

4. The surface modification method of the organic polymer filler for accelerating biofilm formation according to claim 3, characterized in that In the nutrient reagent, the concentration of sodium acetate is (24-36) g / L, the concentration of ammonium sulfate is 2.4 g / L, the concentration of potassium dihydrogen phosphate is 1 g / L, the concentration of trace elements is 100 μL / L, and the concentration of vitamins is 100 μL / L.

5. The method for surface modification of an organic polymer filler for accelerating biofilm formation according to claim 1, characterized in that, The ratio of the addition amount of carrageenan to the addition amount of nutrient reagent in the mixed solution is 10:1 (g:L).

6. The surface modification method of the organic polymer filler for accelerating biofilm formation according to claim 1, characterized in that, The mixed solution also contains a curing agent.

7. The method for surface modification of the organic polymer filler for accelerating biofilm formation according to claim 6, characterized in that, The curing agent is potassium chloride or calcium chloride.

8. The surface modification method of the organic polymer filler for accelerating biofilm formation according to claim 1, characterized in that The organic polymer filler is selected from one or more of polyurethane, polyethylene and polyvinyl chloride.

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