A multi-pass whitening composition and methods of making and using the same

Abstract

The present application relates to a kind of multi-pass whitening composition and its preparation method and application, belong to daily application technical direction.The present application uses glabridin, tea extract, tranexamic acid, ascorbic acid derivative, nicotinamide, hydroxyethyl piperazine ethane sulfonic acid, cholla stem extract, Saxifraga extract and the like to prepare composition, the above-mentioned raw materials are matched, have the effect of inhibiting melanin production and transport, promote the update of keratinocyte and anti-glycation, realize multi-pass whitening, have the effect of high-efficiency whitening, and have higher safety, can effectively give skin healthy, tight effect.

Description

TECHNICAL FIELD

[0001] The present application relates to a multi-channel whitening composition and its preparation method and application, belonging to the application technology direction of daily chemical. BACKGROUND

[0002] The principle of whitening in cosmetics mainly revolves around inhibiting melanin production, blocking melanin transmission, accelerating melanin metabolism, and antioxidant and anti-inflammatory mechanisms. Inhibiting the production of melanin: melanin is synthesized by melanocytes in the basal layer of the skin, and the core step is the activation of tyrosinase. Whitening ingredients intervene in the following ways: inhibiting tyrosinase activity: tyrosinase is a key enzyme in melanin synthesis, and inhibiting its activity can reduce melanin production, including arbutin, kojic acid, transaminic acid (tromethamine), azelaic acid (rhododendric acid), and phenylethyl resorcinol. Reduce oxidative reactions: melanin formation requires an oxidation reaction (such as dopa → dopaquinone), and antioxidants can block this process, including vitamin C (ascorbic acid) and its derivatives, vitamin E, glutathione, and ferulic acid. Block the transmission of melanin even after melanin is produced, it can also prevent its transfer to the epidermal layer: interfere with melanosome transport: melanin is transported to keratinocytes through cell synapses, and some ingredients can block this process, including niacinamide (vitamin B3) and soybean extract. Accelerate melanin metabolism, promote epidermal turnover, or directly strip keratinocytes containing melanin: promote keratin exfoliation: accelerate melanin excretion through exfoliation, including fruit acids (glycolic acid, lactic acid), hydroxyethyl piperazine ethane sulfonic acid, salicylic acid, and enzymes (proteases). Enhance cell turnover: stimulate skin metabolism and reduce epidermal pigment deposition, including retinol (vitamin A derivative). Antioxidants and anti-inflammatory agents neutralize free radicals: ultraviolet (UV) light can induce free radicals, stimulate melanin production, and antioxidants can reduce oxidative stress, including vitamin C, astaxanthin, and coenzyme Q10; reduce inflammatory response: inflammation (such as acne and sunburn) can cause pigmentation (PIH), including dipotassium glycyrrhizinate, bisabolol, and asiaticoside extract. Other auxiliary mechanisms: inhibit melanocyte activation signals: such as inhibiting the binding of alpha-MSH (melanocyte-stimulating hormone) to the receptor, including certain plant extracts (such as licorice root extract); physical concealment: temporarily brighten skin tone with titanium dioxide, mica, and the like.

[0003] There are many research topics related to whitening raw materials in cosmetics, but the whitening products or raw materials on the market generally only have a single whitening channel, which has certain limitations in efficacy, making it difficult to meet consumers' pursuit of high-efficiency whitening. Therefore, developing a combination of multiple whitening active ingredients to synergize multiple different mechanisms of action and providing a safe, multi-dimensional, and multi-channel whitening composition as a cosmetic raw material is still a technical problem that needs to be solved at present. SUMMARY

[0004] In view of the above technical problems, the present application provides a multi-channel whitening composition, a preparation method and application thereof, and belongs to the technical field of daily chemical application.

[0005] The present application provides a multi-channel whitening composition, and raw materials thereof are: glabridin, tea extract, tranexamic acid, ascorbic acid derivative, nicotinamide, hydroxyethylpiperazine ethane sulfonic acid, Opuntia stricta stem extract, Saxifraga extract. The above raw materials are matched, which has the effects of inhibiting melanin production and transport, promoting the renewal of stratum corneum cells and anti-glycation, realizing multi-channel whitening, and having high whitening effect.

[0006] Further, the multi-channel whitening composition, according to weight parts, raw materials are: glabridin 0.0001-0.5 parts, tea extract 0.0001-10.0 parts, tranexamic acid 0.0001-5.0 parts, ascorbic acid derivative 0.0001-5.0 parts, nicotinamide 0.001-10.0 parts, hydroxyethylpiperazine ethane sulfonic acid 0.0001-10.0 parts, Opuntia stricta stem extract 0.0001-10.0 parts, Saxifraga extract 0.0001-10.0 parts.

[0007] Further, the ascorbic acid derivative includes at least one of ascorbic acid phosphate, ascorbic acid glucoside, ascorbic acid palmitate, ascorbic acid tetraisopalmitate and 3-O-ethyl ascorbic acid ether.

[0008] Further, the polyhydric alcohol includes at least one of ethylene glycol, propylene glycol, butylene glycol, glycerol, polyethylene glycol and sugar alcohol.

[0009] Further, the multi-channel whitening composition further includes water, polyhydric alcohol, Epiphyllum oxycostum extract and Amethyst orchid extract.

[0010] Further, the multi-channel whitening composition further includes water 200-2000 parts, polyhydric alcohol 25-200 parts, Epiphyllum oxycostum extract 0.0001-10.0 parts and Amethyst orchid extract 0.0001-10.0 parts.

[0011] Further, the multi-channel whitening composition, according to weight parts, raw materials are: glabridin 0.08-0.5 parts, tea extract 0.2-5 parts, tranexamic acid 0.01-2.0 parts, ascorbic acid derivative 0.1-2.0 parts, nicotinamide 0.1-2 parts, hydroxyethylpiperazine ethane sulfonic acid 0.08-1.0 parts, Opuntia stricta stem extract 0.2-5 parts, Saxifraga extract 0.2-5 parts, Epiphyllum oxycostum extract 0.2-5 parts, Amethyst orchid extract 0.2-5 parts, polyhydric alcohol 10-50 parts and water 100-500 parts.

[0012] Further, the preparation method of the tea leaf extract comprises the following steps: drying tea leaves, crushing, adding water to decoct, stirring at a reduced temperature, filtering, concentrating and drying.

[0013] Further, the preparation method of the Opuntia dillenii Haw stem extract comprises the following steps: drying Opuntia dillenii Haw stems, crushing, adding 20-35wt% ethanol aqueous solution to heat and leach, then filtering, concentrating and drying.

[0014] Further, the preparation method of the Jonquil extract comprises the following steps: drying Jonquil, crushing, adding 20-35wt% ethanol aqueous solution to heat and leach, then filtering, concentrating and drying.

[0015] Further, the preparation method of the Saxifrage extract comprises the following steps: drying Saxifrage, crushing, adding 18-25wt% methanol aqueous solution to heat and leach, then filtering, concentrating and drying.

[0016] Further, the preparation method of the Magnolia liliflora Desch flower extract comprises the following steps: drying Magnolia liliflora Desch flowers, crushing, adding 18-25wt% methanol aqueous solution to heat and leach, then filtering, concentrating and drying.

[0017] Further, the weight ratio of the Jonquil extract and the Magnolia liliflora Desch flower extract is 1:0.7-1.4.

[0018] The application further provides a preparation method of the multi-channel whitening composition, comprising the steps of adding raw materials into a container and stirring to mix.

[0019] The application further provides the application of the multi-channel whitening composition in preparing whitening cosmetics.

[0020] The application further provides a whitening cosmetic, comprising the multi-channel whitening composition, and the weight concentration of the multi-channel whitening composition in the whitening cosmetic is 0.1-10%. Beneficial effects

[0021] The application adopts glabridin, tea leaf extract, tranexamic acid, ascorbic acid derivative, nicotinamide, hydroxyethyl piperazine ethane sulfonic acid, Opuntia dillenii Haw stem extract, Saxifrage extract and the like to prepare the composition, and the above raw materials are matched, so that the composition has the effects of inhibiting melanin generation and transportation, promoting the renewal of keratinocytes and resisting glycation, realizing multi-channel whitening and having a high whitening effect.

[0022] The composition prepared by the application has high safety and no irritation to the skin.

[0023] The composition prepared by the application effectively inhibits tyrosinase and has a good whitening effect.

[0024] The active ingredients of the present application, such as glabridc acid, ascorbyl tetraisopalmitate, 3-O-ethyl ascorbic acid ether, and nicotinamide, can effectively improve the whitening effect of the composition. The glabridc acid and 3-O-ethyl ascorbic acid ether can synergistically improve the effect of inhibiting tyrosinase and impart high-efficiency whitening effect to the composition.

[0025] The composition prepared by the present application can effectively proliferate skin fibroblasts, promote cell metabolism, promote cell renewal and cell repair, make the skin more compact, and enhance the whitening effect to a certain extent.

[0026] The longiflorum extract and the magnolia liliflora extract prepared by the specific process of the present application can effectively improve the effect of proliferating fibroblasts, and the two have synergistic effect on promoting the proliferation of fibroblasts.

[0027] The longiflorum extract and the magnolia liliflora extract prepared by the specific process of the present application can effectively improve the effect of proliferating fibroblasts, and the two have synergistic effect on promoting the proliferation of fibroblasts. BRIEF DESCRIPTION OF DRAWINGS

[0028] 1、 Figure 1 is a face picture of volunteer A before using the agent 1.

[0029] 2、 Figure 2 is a face picture of volunteer A after using the agent 1 for one month. DETAILED DESCRIPTION

[0030] The present application is further described by the following specific implementation content to further illustrate the scheme and effect of the present application.

[0031] The present application provides a multi-channel whitening composition, the raw materials of which are: glabridc acid, tea extract, ascorbic acid derivative, nicotinamide, hydroxyethyl piperazine ethane sulfonic acid, opuntia dillenii stem extract, and saxifraga extract. The above-mentioned raw materials are matched to inhibit melanin production and transport, promote the renewal of keratinocytes, and have anti-glycation effect, so as to realize multi-channel whitening and have high-efficiency whitening effect.

[0032] Further, the multi-channel whitening composition comprises, by weight: 0.0001-0.5 parts of glabridc acid, 0.0001-10.0 parts of tea extract, 0.0001-5.0 parts of ascorbic acid derivative, 0.001-10.0 parts of nicotinamide, 0.0001-10.0 parts of hydroxyethyl piperazine ethane sulfonic acid, 0.0001-10.0 parts of opuntia dillenii stem extract, and 0.0001-10.0 parts of saxifraga extract.

[0033] Further, the ascorbic acid derivative includes at least one of ascorbic acid phosphoric acid ester salt, ascorbic acid glucoside, ascorbic acid palmitate, ascorbic acid tetraisopalmitate, 3-O-ethyl ascorbic acid ether.

[0034] Further, the polyhydric alcohol includes at least one of ethylene glycol, propylene glycol, butylene glycol, glycerol, polyethylene glycol, sugar alcohol.

[0035] Further, the multi-channel whitening composition, the raw material further includes: water, polyhydric alcohol, immortelle extract, purple magnolia extract.

[0036] Further, the multi-channel whitening composition, the raw material further includes: water 200-2000 parts by weight, polyhydric alcohol 25-200 parts by weight, immortelle extract 0.0001-10.0 parts by weight, purple magnolia extract 0.0001-10.0 parts by weight.

[0037] Further, the multi-channel whitening composition, the raw material is: glabridin 0.08-0.5 parts by weight, tea extract 0.2-5 parts by weight, tranexamic acid 0.01-2.0 parts by weight, ascorbic acid derivative 0.1-2.0 parts by weight, nicotinamide 0.1-2 parts by weight, hydroxyethylpiperazine ethanesulfonic acid 0.08-1.0 parts by weight, Opuntia robusta stem extract 0.2-5 parts by weight, Saxifraga stolonifera extract 0.2-5 parts by weight, immortelle extract 0.2-5 parts by weight, purple magnolia extract 0.2-5 parts by weight, polyhydric alcohol 10-50 parts by weight, and water 100-500 parts by weight.

[0038] Further, the preparation method of the tea extract includes: drying the tea leaves, crushing, adding water to decoct, stirring at low temperature, filtering, concentrating and drying to obtain.

[0039] Further, the preparation method of the Opuntia robusta stem extract includes: drying the Opuntia robusta stem, crushing, adding 20-35wt% ethanol aqueous solution to heat extraction, then filtering, concentrating and drying to obtain.

[0040] Further, the preparation method of the immortelle extract includes: drying the immortelle, crushing, adding 20-35wt% ethanol aqueous solution to heat extraction, then filtering, concentrating and drying to obtain.

[0041] Further, the preparation method of the Saxifraga stolonifera extract includes: drying the Saxifraga stolonifera, crushing, adding 18-25wt% methanol aqueous solution to heat extraction, then filtering, concentrating and drying to obtain.

[0042] Further, the preparation method of the purple magnolia extract includes: drying the purple magnolia, crushing, adding 18-25wt% methanol aqueous solution to heat extraction, then filtering, concentrating and drying to obtain.

[0043] Further, the long-lasting flower extract and the purple magnolia flower extract are in a weight ratio of 1:0.7-1.4.

[0044] The present application also provides a preparation method of the multi-channel whitening composition, which comprises the steps of adding raw materials into a container and mixing by stirring.

[0045] The present application also provides an application of the multi-channel whitening composition in preparing a whitening cosmetic.

[0046] The present application also provides a whitening cosmetic, which comprises the multi-channel whitening composition, and the weight concentration of the multi-channel whitening composition in the whitening cosmetic is 0.1-10%.

[0047] The specific preparation process and test are as follows.

[0048] I. Preparation Example

[0049] The preparation method of the tea leaf extract is as follows: the tea leaf is dried and crushed, 20 times the weight of water is added to boil, and then the temperature is lowered to 50 DEG C to stir at 60 rpm for 4 hours, and then filtered, concentrated under reduced pressure to 17.9% of the weight of the filtrate, and vacuum dried to 2.91wt% of water content.

[0050] The preparation method of the Opuntia stricta stem extract is as follows: the Opuntia stricta stem is dried and crushed, 25wt% ethanol aqueous solution is added according to the solid-liquid weight ratio of 1:25, heated at 55 DEG C and stirred at 70 rpm for 4 hours, and then filtered, concentrated under reduced pressure to 15.7% of the weight of the filtrate, and vacuum dried to 2.55wt% of water content.

[0051] The preparation method of the long-lasting flower extract is as follows: the long-lasting flower is dried and crushed, 22.5wt% ethanol aqueous solution is added according to the solid-liquid weight ratio of 1:20, heated and stirred at 57 DEG C and 80 rpm for 4 hours, and then filtered, concentrated under reduced pressure to 16.3% of the weight of the filtrate, and vacuum dried to 2.07wt% of water content.

[0052] The preparation method of the Saxifraga stolonifera extract is as follows: the Saxifraga stolonifera is dried and crushed, 20wt% methanol aqueous solution is added according to the solid-liquid weight ratio of 1:30, heated and stirred at 35 DEG C and 75 rpm for 4.5 hours, and then filtered, concentrated under reduced pressure to 12.7% of the weight of the filtrate, and vacuum dried to 2.21wt% of water content.

[0053] The preparation method of the purple magnolia flower extract is as follows: the purple magnolia flower is dried and crushed, 22.5wt% methanol aqueous solution is added according to the solid-liquid weight ratio of 1:25, heated and stirred at 35 DEG C and 70 rpm for 3.5 hours, and then filtered, concentrated under reduced pressure to 12.6% of the weight of the filtrate, and vacuum dried to 2.76wt% of water content.

[0054] The raw materials and composition of the preparation are shown in Table 1, and the raw materials are measured by weight parts. The I value is the weight ratio of the tuberose extract and the magnolia liliflora extract.

[0055] Table 1: Composition of the preparation

[0056] No. Agent 1 Agent 2 Agent 3 Agent 4 Agent 5 Glabridin 0.2 0.15 0.2 0.2 0.2 Tea extract 1.0 1.1 1.0 1.0 1.0 Transferrin 0.4 0.5 0 0.8 0 Ascorbyl glucoside 0.1 0.2 0.1 0.1 0.1 Ascorbyl tetraisopalmitate 0.1 0 0 0.1 0.1 3-O-ethyl ascorbic acid ether 0.4 0.3 0 0 0.8 Nicotinamide 0.3 0.3 0 0.3 0.3 Hydroxyethylpiperazine ethane sulfonic acid 0.2 0.2 0.2 0.2 0.2 Opuntia robusta stem extract 1.5 1.4 1.5 1.5 1.5 Saxifraga extract 1.2 1.3 1.2 1.2 1.2 Gymnadenia conopsea extract 2.0 2.4 0 2.0 2.0 Magnolia liliflora extract 2.2 1.8 0 2.2 2.2 1,2-propanediol 3.0 5.0 3.0 3.0 3.0 1,3-butanediol 5.0 0 5.0 5.0 5.0 Glycerol 7.0 10.5 7.0 7.0 7.0 Water 120.2 121.0 125.6 120.2 120.2 I value 1:1.1 1:0.75 / / /

[0057] No. Agent 6 Agent 7 Agent 8 Agent 9 Glabridin 0.2 0.2 0.2 0.2 Tea extract 1.0 1.0 1.0 1.0 Transferrin 0.4 0.4 0.4 0.4 Ascorbyl glucoside 0.1 0.1 0.1 0.1 Ascorbyl tetraisopalmitate 0.1 0.1 0.1 0.1 3-O-ethyl ascorbic acid ether 0.4 0.4 0.4 0.4 Nicotinamide 0.3 0.3 0.3 0.3 Hydroxyethylpiperazine ethane sulfonic acid 0.2 0.2 0.2 0.2 Opuntia robusta stem extract 1.5 1.5 1.5 1.5 Saxifraga extract 1.2 1.2 1.2 1.2 Gymnadenia conopsea extract 4.2 0 3.4 1.0 Magnolia liliflora extract 0 4.2 0.8 3.2 1,2-propanediol 3.0 3.0 3.0 3.0 1,3-butanediol 5.0 5.0 5.0 5.0 Glycerol 7.0 7.0 7.0 7.0 Water 120.2 120.2 120.2 120.2 I value / / 1:0.23 1:3.2

[0058] The preparation method of the above preparation is as follows: the raw materials are added to a container with a stirring paddle, heated to 40 DEG C, and mixed under the condition of 75 rpm stirring speed for 0.6 hours, then room temperature standing for 0.5 hours, to obtain the preparation.

[0059] II. Test

[0060] The performance test is carried out on the above prepared preparation.

[0061] 1. Safety test

[0062] 72 healthy volunteers aged 25-50 years old are recruited and randomly divided into 9 groups according to age, and there are 4 male and female volunteers in each group. The experiment is tested according to the "skin closed patch test" recorded in chapter 7, section 2 of "Cosmetic Safety Technology Standard-2015": 0.020g of the above preparation 1-9 is placed in the patch device and applied to the inner side of the left arm of the subject, and then removed after 24 hours, and then the skin reaction is observed at 1h and 24h. Then the evaluation is carried out according to the "skin reaction grading standard of skin closed patch test" in the section.

[0063] The evaluation test result shows that the number of people with score grade 0 in the preparation 1-9 is 8; the number of people with score grade 1-4 in the preparation 1-9 is 0.

[0064] Therefore, it can be known that the composition prepared by the application has high safety and no skin irritation.

[0065] Whitening test

[0066] The increase of tyrosinase activity usually leads to the increase of melanin production, and when the tyrosinase activity is reduced or inhibited, the production of melanin is reduced, thereby achieving the effect of skin whitening. The application tests the inhibition of tyrosinase activity to test the whitening effect of the product.

[0067] The method for testing the inhibition of tyrosinase in the application is as follows:

[0068] B16 mouse melanoma cells were cultured in RPMI-1640 medium containing 10% FBS at 37°C in a 5% CO2 environment until the exponential growth phase, digested with 0.25% trypsin-EDTA, pipetted, centrifuged and the supernatant discarded, and RPMI-1640 medium was added to adjust the cell density to 8.5×10 5 / mL, 100 μL was added to each well of a 96-well plate and cultured at 37°C, 5% CO2 for 24 h; then 100 μL of RPMI-1640 medium was added to the blank group well plate, and 100 μL of sample solution (RPMI-1640 medium and the above-mentioned preparation in a weight ratio of 40:1) was added to the sample group well plate; then cultured at 37°C, 5% CO2 for 72 h.

[0069] After the incubation period, the supernatant was discarded, and 140 μL of 1 wt% Triton X-100 solution was added to each well. After freezing at -85°C for half an hour, the cells were thawed at room temperature to rupture. Then, 55 μL of 0.5 wt% L-DOPA solution was added to each well in a 37°C water bath. The cells were incubated at 37°C in the dark for 2 hours. The absorbance (OD) was measured at 490 nm using a microplate reader. The tyrosinase inhibition rate (α value / %) was calculated as 100% × [1 - sample group OD value / blank group OD value]. The α value reduction (Δα) of each group compared to that of agent 1 was also calculated. The test results are shown in Table 2.

[0070] Table 2: Whitening test

[0071] No. Alpha value / % Delta alpha value / % Agent 1 42.36 / Agent 2 40.91 1.45 Agent 3 23.75 18.61 Agent 4 37.13 5.23 Agent 5 36.05 6.31 Agent 6 41.29 1.07 Agent 7 39.05 3.31 Agent 8 40.82 1.54 Agent 9 39.74 2.62

[0072] As shown in Table 2, the compositions prepared by the present invention effectively inhibit tyrosinase and exhibit a good whitening effect. Testing of binders 1 and 3 shows that the active ingredients used in the present invention, such as tranexamic acid, ascorbyl tetraisopalmitate, 3-O-ethyl ascorbyl ether, and niacinamide, can effectively enhance the whitening effect of the composition. Testing of binders 1, 4, and 5 shows that tranexamic acid and 3-O-ethyl ascorbyl ether can synergistically and progressively enhance the tyrosinase inhibition effect of the composition, imparting a highly effective whitening effect to the composition.

[0073] 3. Fibroblast proliferation test

[0074] Fibroblast proliferation can increase collagen content, reduce skin laxity and wrinkles caused by photoaging, make skin color more uniform, and have the function of delaying skin aging. In addition, it can also reduce inflammatory reactions (such as UV damage or post-acne inflammation), thereby reducing excessive deposition of melanin, and indirectly affecting melanocyte activity and down-regulating tyrosinase expression by secreting factors KGF, IL-6, etc. It can also enhance keratinocyte metabolism, accelerate the shedding of keratinocytes containing melanin, secrete antioxidant enzymes (such as superoxide dismutase), reduce UV-induced reactive oxygen species (ROS) damage, and thus reduce the stimulation of ROS on melanocytes.

[0075] The method for testing the effect of the product of the present application on fibroblast proliferation is as follows:

[0076] L929 fibroblasts were cultured in RPMI-1640 medium containing 10% FBS at 37°C in a 5% CO2 environment to the exponential growth phase, digested with 0.25% trypsin-EDTA, blown and centrifuged to discard the supernatant, and then RPMI-1640 medium was added to adjust the cell concentration to 6.5×10 4 Each well was inoculated with 100 μL, and 100 μL of RPMI-1640 medium was added to each well of the blank group, and 100 μL of sample solution was added to each well of the sample group. The sample solution was prepared by mixing RPMI-1640 medium and the above preparation at a weight ratio of 100:1. Each well was further cultured at 37°C in a 5% CO2 environment for 72 h.

[0077] 15 μL of 0.65 mg / L MTT was added to each well and incubated for 3.5 h, and then the supernatant was removed and 200 μL of DMSO was added to each well and shaken in a 37°C water bath for 20 min. The absorbance OD value was measured at 570 nm using a microplate reader, the relative proliferation rate was calculated, the relative proliferation rate β value (%) = 100%×(OD experimental group / OD blank group-1), and the average value was calculated. The reduction value Δβ value of each group compared to agent 1β was calculated, and the results are shown in Table 3.

[0078] Table 3: Skin cell proliferation test

[0079] No. Beta value / % Delta beta value / % Agent 1 38.9 / Agent 2 37.1 1.8 Agent 3 22.8 16.1 Agent 4 36.7 2.2 Agent 5 36.1 2.8 Agent 6 31.5 7.4 Agent 7 32.3 6.6 Agent 8 35.4 3.5 Agent 9 36.2 2.7

[0080] The results of the tests in Table 3 show that the composition prepared by the present application can effectively proliferate skin fibroblasts, promote cell metabolism, promote cell renewal and cell repair, make the skin more compact, and enhance the whitening effect to a certain extent. The tests of the binders 1, 6 and 7 show that the long-lasting flower extract and the purple magnolia flower extract prepared by the specific process of the present application can effectively improve the effect of the composition on proliferating fibroblasts, and the two have a synergistic effect on promoting the proliferation of fibroblasts. The binders 1 and 2 with the weight ratio of long-lasting flower extract to purple magnolia flower extract being 1:0.7-1.4, especially the binder 1, have a higher efficiency and better effect on proliferating fibroblasts than the binders 7-8 without the above-mentioned ratio, which shows that the selection of the weight ratio of long-lasting flower extract to purple magnolia flower extract being 1:0.7-1.4 can improve the proliferation of fibroblasts and give the skin a more youthful and energetic state.

[0081] 4. Whitening test

[0082] Eight volunteers aged 30-50 years old were recruited to use the product of the binder 1. After washing the face every night, 0.8g of the sample of the binder 1 was applied to the face, and the effect was evaluated after continuous use for one month. The observation of the facial skin showed that the skin color of five volunteers was whitened to a certain extent. Among them, Figure 1 and Figure 2 is a volunteer (A) before and after using the above binder 1.

Claims

1. A multi-pass whitening composition, characterized in that, The raw materials consist of the following components by weight: 0.08-0.5 parts of glabridin, 0.2-5 parts of tea extract, 0.01-2.0 parts of tranexamic acid, 0.1-2.0 parts of ascorbic acid derivative, 0.1-2 parts of nicotinamide, 0.08-1.0 parts of hydroxyethylpiperazineethanesulfonic acid, 0.2-5 parts of Opuntia dillenii Haw stem extract, 0.2-5 parts of Saxifraga stolonifera Meerb extract, 0.2-5 parts of Kalanchoe pinnata extract, 0.2-5 parts of Magnolia liliflora extract, 10-50 parts of polyhydric alcohol, and 100-500 parts of water; The ascorbic acid derivative is at least one of ascorbic acid phosphate, ascorbic acid glucoside, ascorbic acid palmitate, ascorbic acid tetraisopalmitate, and 3-O-ethyl ascorbic acid ether; The Saxifraga stolonifera Meerb extract and the Magnolia liliflora extract are in a weight ratio of 1:0.7-1.

4.

2. The multi-pass whitening composition of claim 1, wherein, The preparation method of the tea extract comprises the following steps: drying tea leaves, crushing, adding water for decocting, stirring at a reduced temperature, filtering, concentrating, and drying.

3. The multi-pass whitening composition of claim 1, wherein, The preparation method of the Opuntia dillenii Haw stem extract and the Kalanchoe pinnata extract comprises the following steps: drying Opuntia dillenii Haw stems or Kalanchoe pinnata, crushing, adding 20-35 wt% ethanol aqueous solution for heating and leaching, then filtering, concentrating, and drying. Or, the preparation method of the Saxifraga stolonifera Meerb extract and the Magnolia liliflora extract comprises the following steps: drying Saxifraga stolonifera Meerb or Magnolia liliflora, crushing, adding 18-25 wt% methanol aqueous solution for heating and leaching, then filtering, concentrating, and drying.

4. A process for the preparation of a multi-pass whitening composition according to any one of claims 1 to 3, characterized in that, The method comprises the step of adding the raw materials into a container and stirring to mix.

5. Use of the multi-channel whitening composition according to any one of claims 1-3 in the preparation of a whitening cosmetic.

6. A whitening-type cosmetic, characterized by comprising: The multi-channel whitening composition according to any one of claims 1-3 is used in the whitening cosmetic at a weight concentration of 0.1-10%.

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