A mixed biological bacterial fertilizer, a preparation method and application thereof

Abstract

The application discloses a mixed biological bacterial fertilizer, a preparation method and application thereof. The application is based on the three major nutrient elements of nitrogen, phosphorus and potassium which are necessary for plant growth, and eight kinds of bacteria are compounded, including two kinds of phosphorus solubilizing bacteria, i.e., soil olive-shaped bacteria and protomonospora; three kinds of potassium solubilizing bacteria, i.e., Cabriales Bacillus, Siamese Bacillus and Beiles Bacillus; and three kinds of nitrogen-fixing bacteria, i.e., lemon protomonospora, Yunnan protomonospora and Korean pseudomonas. The mixed biological bacterial fertilizer is prepared by combining the eight kinds of growth-promoting bacteria with kitchen garbage and biomass waste, and meets the growth demand of plants. The sunflower, clematis and sunflower are planted in pots. The bacterial fertilizer has the advantages of fast germination, high germination rate, fast growth and healthy and dense leaves. The biological organic fertilizer has the advantages of green and environmental protection, and lays a theoretical foundation for replacing traditional chemical fertilizer with the biological organic fertilizer.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial fertilizer preparation, in particular, discloses a mixed biological bacterial fertilizer and a preparation method and application thereof. BACKGROUND

[0002] Nitrogen, phosphorus and potassium are the three most active nutrients in the process of plant growth and development, and play an irreplaceable physiological role in the growth cycle of crops. Among them, nitrogen is an essential nutrient for plant growth, and is the main component of proteins, nucleic acids and chlorophyll in plants. It has a significant regulatory effect on plant growth and development. When the supply of nitrogen is sufficient, more proteins can be synthesized in the plant body, promoting cell division and growth, thereby increasing leaf area and enhancing photosynthetic efficiency. Light energy is converted into chemical energy, and efficient conversion of carbon dioxide and water into organic compounds such as glucose is achieved.

[0003] The content of phosphorus in plants is second only to nitrogen and potassium, and is generally higher in seeds. As a non-renewable constant nutrient element, phosphorus not only participates in plant energy metabolism and material synthesis, but also significantly improves the stress resistance of crops and enhances their ability to resist adverse environments such as cold and drought. Lack of phosphorus will cause slow growth and yield reduction of crops, while excessive phosphorus may cause late maturity and low seed setting rate. Potassium is one of the three most deficient nutrients in soil, and is distributed in organs and tissues with high metabolic activity. Unlike nitrogen and phosphorus, it is not a component of organic compounds in plants and does not directly participate in the construction of plant organic matter. Because potassium ions can accumulate in crop cells, they can promote water transport from soil to roots by regulating cell osmotic pressure. When the supply of potassium is sufficient, crops can effectively use water and reduce transpiration, thereby enhancing the drought resistance of crops. The coordinated supply of these three nutrients plays a decisive role in the growth and development, yield formation and quality improvement of crops.

[0004] Bacterial fertilizer, also known as biological fertilizer, microbial fertilizer, bacterial fertilizer or inoculant, is a new type of fertilizer product developed based on the principles of soil microecology, plant nutrition and the basic concepts of modern "organic agriculture". As a new type of fertilizer in the twenty-first century, microbial bacterial fertilizer is a biological product produced by modern biological engineering technology, which is a low-carbon, environmentally friendly, pure natural, non-toxic, harmless and pollution-free organic microbial agent. In recent years, through genetic engineering, synthetic biology and other technologies, the screening and improvement of high-efficiency strains have made significant progress. In addition, the development of composite functional strains has become a trend, such as the combination of nitrogen-fixing bacteria, phosphorus-dissolving bacteria and disease-resistant bacteria, which can simultaneously improve soil structure, promote nutrient absorption and inhibit soil-borne diseases. With the increasing attention to agricultural ecology and sustainable development around the world, biological bacterial fertilizer, with its environmental friendliness and efficient use, shows a broad application prospect. In production, by utilizing the unique metabolic function of microorganisms, various nutrients that promote plant growth can be effectively synthesized, plant growth is promoted, plant nutrient structure is optimized, and the sustainable development of agricultural production is thus promoted. SUMMARY

[0005] Technical problem to be solved: The field performance of microbial fertilizer is significantly affected by factors such as soil temperature, humidity and pH value, and the activity of most strains decreases. In addition, residual chemical pesticides and heavy metals in the soil may inhibit microbial survival, leading to fluctuating effects. The present application is based on the identification of bacteria from soil microorganisms, and eight functional microbial strains are compounded to cultivate potted plants using biomass waste as a bacterial fertilizer carrier, providing an innovative fertilizer solution for modern agriculture and promoting the green transformation and high-quality development of agricultural production.

[0006] Technical solution: The present application provides a mixed biological bacterial fertilizer, comprising a microbial flora and a bacterial fertilizer carrier; the microbial flora is a composite bacteria composed of nitrogen-fixing bacteria, potassium-dissolving bacteria and phosphorus-dissolving bacteria.

[0007] Further, the volume ratio of nitrogen-fixing bacteria, potassium-dissolving bacteria and phosphorus-dissolving bacteria in the composite bacteria is (2-8):(1-6):(2-5).

[0008] Further, the nitrogen-fixing bacteria include Prosthecochloris aminophila (P. aminophila), Prosthecochloris vancanrensis (P. vancanrensis) and Pseudomonas koreensis (P. koreensis) mixed in a volume ratio of (2-4):(1-5):(1-5). Promicromonospora citrea Promicromonospora yunnanensis Pseudomonas sp.

[0009] Further, the bacterial suspension concentration of P. aminophila is 3.0x10 7 -1.0x10 9 CFU / mL, and the bacterial suspension concentration of P. vancanrensis is 2.0x10 6 -1.0x10​​​9 CFU / mL, the concentration of the bacterial suspension of Pseudomonas koreensis was 1.9 x 10 6 -1.0 x 10 9 CFU / mL.

[0010] Further, the phosphorus solubilizing bacteria include soil olive-shaped bacteria (1-2) and Prosthecobacter validus (1-3) mixed in a volume ratio of (1-2):(1-3):(1-2). Olivibacter

[0011] soli Promicromonospora sp. .

[0012] Further, the concentration of the bacterial suspension of soil olive-shaped bacteria was 1.8 x 10 6 -1.0 x 10 9 CFU / mL, the concentration of the bacterial suspension of Prosthecobacter validus was 4.0 x 10 6 -2.0 x 10 9 CFU / mL.

[0013] Further, the potassium solubilizing bacteria include Bacillus aryabhattai (1-2), Bacillus siamensis (1-3) and Bacillus velezensis (1-2) mixed in a volume ratio of (1-2):(1-3):(1-2). Bacillus cabrialesii Bacillus siamensis Bacillus velezensis

[0014] Further, the concentration of the bacterial suspension of Bacillus aryabhattai was 1.0 x 10 7 -3.0 x 10 9 CFU / mL, the concentration of the bacterial suspension of Bacillus siamensis was 2.0 x 10 8 -1.0 x 10 9 CFU / mL, and the concentration of the bacterial suspension of Bacillus velezensis was 1.0 x 10 7 -7.0 x 10 8 CFU / mL.

[0015] Further, the microbial fertilizer carrier is biomass waste or garbage.

[0016] Further, the biomass waste is wheat bran, and the garbage is kitchen garbage.

[0017] The preparation method of the high-efficiency mixed microbial fertilizer includes the following steps: activating and culturing nitrogen-fixing bacteria, potassium solubilizing bacteria and phosphorus solubilizing bacteria, and adding the mixed suspension into a microbial fertilizer carrier to obtain the mixed microbial fertilizer.

[0018] The application of the mixed microbial fertilizer in pot planting.

[0019] Further, the pot planting is the planting of Catharanthus roseus, sunflower and helianthus.​​​​ Advantages

[0020] The bacteria used in the bacterial fertilizer are based on the bacteria identified in the soil, which improves the problem of microbial activity decline in potting, and the bacterial fertilizer carrier material used in the preparation process is cheap waste biomass material such as wheat bran and kitchen garbage, which reduces the cost of bacterial fertilizer preparation process and is simple to prepare.

[0021] The microbial fertilizer is rich in nutrients, and after potting and planting of various flowers, the germination rate, germination speed and growth speed of the plants are significantly improved, and the effect significantly promotes the growth of the plants.

[0022] The application improves the utilization rate of nitrogen, phosphorus, potassium and trace elements in the soil by the cooperation of different strains, optimizes the effect of single microorganism, and improves plant growth.

[0023] The compound bacteria can produce antibacterial active substances (such as antibiotic analogues), inhibit part of soil-borne pathogenic fungi (such as fusarium), and secrete plant hormones (such as auxin and cytokinin), stimulate root development, and enhance the stress resistance of crops (such as drought resistance and salt resistance). BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a process flow chart for preparing the mixed biological bacterial fertilizer;

[0025] Figure 2 It is a schematic diagram of the growth stage of sunflower in the effect verification of the control group and example 1;

[0026] Figure 3 It is a schematic diagram of the growth stage of the longflower in the effect verification of the control group and example 2;

[0027] Figure 4 It is a schematic diagram of the growth stage of sunflower in the effect verification of the control group and example 3. DETAILED DESCRIPTION

[0028] The application will be further described below in combination with the drawings and examples, and the following examples are an explanation of the application, and the application is not limited to the following examples: the detailed information of the strains used in the compound bacterial fertilizer is shown in table 1.

[0029] Table 1 detailed information of 8 strains in the compound bacterial fertilizer

[0030] Example

[0031] Application of mixed biological bacterial fertilizer in sunflower potting:

[0032] Preparation of bacterial fertilizer: First, sterilize wheat bran at 121°C for 30 minutes each time, with six cycles of intermittent sterilization. Before planting, add 500g of soil and 10g of wheat bran to each pot. After activation, the colony counts and dosages of each bacterial agent are shown in Table 2. Sterile water was used instead of the bacterial strains as a control.

[0033] Table 2 Composition of bacteria in sunflower potted fertilizer

[0034]

[0035] Sunflower planting: The temperature during planting is room temperature, which ranges from 19 ºC to 30 ºC, and the humidity is (42-80)% RH. Water appropriately according to the room temperature, humidity and weather conditions.

[0036] like Figure 2 As shown in Table 3, significant differences were observed in germination and growth trends between the experimental and control groups. As shown in Table 3, the potted plants treated with the composite strain showed significantly better germination potential and germination rate than the control group, and the seedlings also grew faster than the control group. This flower species exhibited slower growth and thinner flower stems.

[0037] Table 3 Growth of sunflower potted plants

[0038] Example

[0039] Application of mixed biological fertilizer in potted Catharanthus roseus:

[0040] Preparation of bacterial fertilizer: First, sterilize wheat bran at 121°C for 30 minutes each, with six cycles of intermittent sterilization. Before planting, add 750g of soil and 10g of wheat bran to each pot. The colony counts and dosages for each test strain are shown in Table 4. A control group was prepared using sterile water instead of the strains.

[0041] Table 4 Composition of bacteria in Catharanthus roseus potted bacterial fertilizer

[0042]

[0043] Catharanthus roseus planting: During the growth period, the room temperature is above 25 ºC, the temperature range is 25 ºC-33 ºC, and the humidity range is (48-78)%RH. Water appropriately according to the room temperature, humidity and weather conditions.

[0044] like Figure 3 As shown in Table 5, the germination rate and growth of the potted plants with the addition of the composite strain were significantly better than those of the control group. After a period of growth, the growth height of the potted plants with the addition of the composite strain was also significantly better than that of the control group.

[0045] Table 5 Growth of Catharanthus roseus potted plants

[0046] Example

[0047] Application of mixed biological fertilizer in sunflower potted plants:

[0048] Preparation of biofertilizer: First, sterilize wheat bran and kitchen waste at 121°C for 30 minutes each, with six cycles of intermittent sterilization. Before planting, add 750g of each fertilizer to each pot, including 5g of wheat bran and 15g of kitchen waste. The colony counts and dosages for each test strain are shown in Table 6. Sterile water was used instead of the strains as a control.

[0049] Table 6 Composition of bacteria in sunflower potted fertilizer

[0050]

[0051] Sunflower planting: During the growth period, the room temperature range is 20 ºC-30 ºC, and the humidity range is (42-76)%RH. Water appropriately according to the room temperature, humidity and weather conditions.

[0052] like Figure 4 As shown in Table 7, the potted plants with the composite strain grew better than the control group, while the leaves of the seedlings in the control group with sterile water turned yellow. As shown in Table 7, the sunflower seedlings of the embodiment grew taller and had larger leaves than the control group, and the final flower disk was also larger.

[0053] Table 7 Growth of sunflower potted plants

[0054]

[0055] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Any person skilled in the art can, without departing from the spirit and technical solution of the present invention, make many possible changes and modifications to the technical solution of the present invention using the methods and technical contents disclosed above, or modify it into an equivalent embodiment with equivalent changes. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention shall still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A mixed biological fertilizer, characterized by: The invention comprises a microbial flora and a bacterial fertilizer carrier; the microbial flora is a composite bacteria composed of nitrogen-fixing bacteria, potassium-solubilizing bacteria and phosphate-solubilizing bacteria in a volume ratio of (2-8): (1-6): (2-5); the nitrogen-fixing bacteria include Micromonospora citriodora, Micromonospora yunnanensis and Pseudomonas koreacens mixed in a volume ratio of (2-4): (1-5): (1-5); the bacterial suspension concentration of Micromonospora citriodora is 3.0×10 7 -1.0×10 9 CFU / mL, the concentration of the bacterial suspension of Micromonospora yunnanensis was 2.0×10 6 -1.0×10 9 CFU / mL, the concentration of the Korean Pseudomonas bacterial suspension was 1.9×10 6 -1.0×10 9 CFU / mL; the phosphate-solubilizing bacteria include soil olive-shaped bacteria and Protomicromonospora mixed in a volume ratio of (1-3): (1-2), and the concentration of the soil olive-shaped bacteria suspension is 1.8×10 6 -1.0×10 9 CFU / mL, the concentration of the bacterial suspension of Micromonospora protozoa was 4.0×10 6 -2.0×10 9 CFU / mL; the potassium-dissolving bacteria include Bacillus cabriae, Bacillus siamese and Bacillus velez mixed in a volume ratio of (1-2): (1-3): (1-2), and the bacterial suspension concentration of Bacillus cabriae is 1.0×10 7 -3.0×10 9 CFU / mL, the concentration of the siam bacillus suspension was 2.0×10 8 -1.0×10 9 CFU / mL, the concentration of the bacterial suspension of Bacillus velezensis was 1.0×10 7 -7.0×10 8 CFU / mL.

2. A mixed biological fertilizer according to claim 1, characterized in that: The bacterial fertilizer carrier is biomass waste or garbage.

3. A mixed biological fertilizer according to claim 2, characterized in that: The biomass waste is wheat bran; and the garbage is kitchen waste.

4. A method for preparing a mixed biological fertilizer according to any one of claims 1 to 3, characterized in that: The nitrogen-fixing bacteria, potassium-solubilizing bacteria and phosphate-solubilizing bacteria are activated and cultured, and the mixed suspension is added into a bacterial fertilizer carrier to obtain the product.

5. Use of a mixed biological fertilizer according to any one of claims 1 to 3 in potted planting.

6. The use according to claim 5, characterized in that: The potted planting is the planting of vinca roseus, sunflower and sunflower.

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