Polypeptide chip sample automatic detection processing equipment and detection method

Abstract

The invention discloses polypeptide chip sample automatic detection processing equipment and a detection method. The polypeptide chip sample automatic detection processing equipment comprises a feeding assembly, a liquid transferring assembly, an incubation assembly, a cleaning assembly and a visual detection assembly, and the liquid transferring assembly, the incubation assembly, the cleaning assembly and the visual detection assembly are correspondingly arranged on a liquid transferring station, an incubation station, a cleaning station and a visual detection station respectively. The feeding assembly operates among the feeding station, the liquid transferring station, the incubation station, the cleaning station, the visual detection station and the discharging station and is used for conveying chip samples and reagents needed by detection. According to the invention, human intervention can be reduced, the efficiency and reliability of biochip detection can be improved, and the biochip detection can be rapidly and accurately carried out, and meanwhile, the biochip detection device has obvious advantages in detection convenience and privacy.

Description

Technical Field

[0001] The present invention particularly relates to an automatic detection and processing device and a detection method for a polypeptide chip sample, belonging to the technical field of chip detection devices. Background Art

[0002] Biochips have become important tools for life science research and medical diagnosis. Due to their advantages such as high throughput, parallelism, and low sample consumption, they are often used in fields such as medical diagnosis, genomics research, drug screening, and biomedical research.

[0003] Biochips can be produced by semiconductor processes. A high-density polypeptide chip synthesized on the surface of a silicon wafer using a semiconductor process is characterized by small individual polypeptide sequence spots, usually 1 to 100 micrometers, preferably 5 to 20 micrometers; high density, and tens of thousands to hundreds of thousands of different polypeptide sequence dot matrices can be in-situ synthesized at the bottom of a single well of a 96-well plate. In a chip synthesized using a semiconductor process, the area of the chip directly affects the production cost. This means that when producing the chip, it is necessary to make the most of the chip area as much as possible to accommodate more probes with detection indicators per unit area, which can be used to analyze more molecules or proteins in a biological sample, thereby reducing the cost of a single detection indicator.

[0004] During the detection process of a biochip, it needs to be first loaded into a well plate fixture, and then reactions are carried out. Signals are collected and imaged by scanning it with a microscope. The well plate for loading the chip provides the functions of fixing the chip and sealing during the detection process. In order to achieve more efficient detection, a more advanced optical system needs to be used during imaging scanning to improve the magnification and fluorescence collection efficiency, because the silicon wafer is opaque and cannot be detected from the back.

[0005] In the current detection systems for common 96-well plates, when detecting from above, there are often problems such as insufficient working distance of high-power microscopes, making it difficult to obtain clear images. Especially for areas near the well walls, due to the influence of surface tension, the liquid surface shows a concave or convex curved shape, which seriously affects the imaging quality of the chip and cannot fully utilize the bottom area of a single well to achieve more index detections. Therefore, it is necessary to remove the well plate of the chip before using a fluorescence objective lens with a higher numerical aperture for detection. This step usually requires the tester to manually use a screwdriver to remove the well plate fixture. In addition to loading and unloading the fixture, in traditional biochip detections, multiple manual steps are usually required, including pre-treatment of the chip, reagent addition (samples and reagents required for the experiment), transfer of the fixture during the experimental steps, and detection and recording of results. These manual steps often require professional operators. During the operation process, due to operator errors, sample contamination, and increased processing time, the accuracy and efficiency of detection are reduced, and multiple uncertain factors are introduced. These uncertain factors brought by manual steps not only make it impossible to fully reproduce the test process, but also when abnormal detection results occur, it is impossible to analyze and trace the changes in experimental operations and the sources of problems. Summary of the Invention

[0006] The main object of the present invention is to provide an automatic detection and processing device and detection method for polypeptide chip samples, so as to overcome the deficiencies in the prior art.

[0007] To achieve the foregoing invention object, the technical solutions adopted by the present invention include:

[0008] On the one hand, the present invention provides an automatic detection and processing device for polypeptide chip samples, including:

[0009] A chip cartridge for carrying the chip and jointly forming a chip sample with the chip;

[0010] A feeding component at least for conveying the chip sample and the reagents required for detection between a feeding station, a pipetting station, an incubation station, a cleaning station, a vision detection station, and a discharging station;

[0011] A pipetting component disposed at the pipetting station, the pipetting component at least for adding the reagents required for detection to the chip sample located at the pipetting station;

[0012] An incubation component disposed at the incubation station, the incubation component at least for incubating the chip sample after adding the reagents required for detection;

[0013] A cleaning component disposed at the cleaning station, the cleaning component at least for cleaning the chip sample that has completed incubation;

[0014] The vision detection component is arranged at the vision detection station, and the graphic detection component is at least used to obtain the image information of the chip sample that has completed incubation.

[0015] On the other hand, the present invention also provides a method for automatically detecting and processing a polypeptide chip sample, and the method for automatically detecting and processing a polypeptide chip sample is implemented based on the device for automatically detecting and processing a polypeptide chip sample.

[0016] Compared with the prior art, the advantages of the present invention include: The device for automatically detecting and processing a polypeptide chip sample provided by the present invention can reduce human intervention, improve the efficiency and reliability of biochip detection, ensure rapid and accurate biochip detection, and is also applicable to the application scenario in the home environment. It eliminates the need to queue for a long time in a medical institution, and a series of steps such as sampling, sample processing, detection, and obtaining a report can be completed at home, having obvious advantages in terms of detection convenience and privacy. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of a device for automatically detecting and processing a polypeptide chip sample provided in a typical embodiment of the present invention;

[0018] Figure 2 It is a schematic structural diagram of a device for automatically detecting and processing a polypeptide chip sample provided in a typical embodiment of the present invention;

[0019] Figure 3 It is a schematic structural diagram of a chip cartridge provided by the present invention;

[0020] Figure 4 It is a schematic structural diagram of the loading component in the present invention;

[0021] Figure 5 It is a schematic structural diagram of the pipetting component in the present invention;

[0022] Figure 6 It is a schematic structural diagram of the incubation component in the present invention;

[0023] Figure 7 It is a schematic structural diagram of the cleaning component in the present invention;

[0024] Figure 8 It is a schematic structural diagram of the vision detection component in the present invention;

[0025] Figure 9 It is a schematic flow diagram of a method for automatically detecting a polypeptide chip sample provided in a typical embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] In view of the deficiencies in the prior art, through long-term research and extensive practice, the inventors of this case have been able to propose the technical solution of the present invention. The following will further explain the technical solution, its implementation process, principles, etc.

[0027] On the one hand, the present invention provides an automatic detection and processing device for polypeptide chip samples, including:

[0028] A chip cartridge for carrying the chip and jointly forming a chip sample with the chip;

[0029] A feeding component at least for transporting the chip sample and the reagents required for detection between the feeding station, the pipetting station, the incubation station, the cleaning station, the vision detection station and the discharging station;

[0030] A pipetting component arranged at the pipetting station, and the pipetting component is at least used for adding the reagents required for detection to the chip sample located at the pipetting station;

[0031] An incubation component arranged at the incubation station, and the incubation component is at least used for incubating the chip sample after adding the reagents required for detection;

[0032] A cleaning component arranged at the cleaning station, and the cleaning component is at least used for cleaning the chip sample that has completed incubation;

[0033] A vision detection component arranged at the vision detection station, and the graphic detection component is at least used for obtaining the image information of the chip sample that has completed incubation.

[0034] Furthermore, the feeding component includes a conveying mechanism and a cartridge. The conveying mechanism at least sequentially passes through the pipetting station, the incubation station, the cleaning station, the vision detection station from the feeding station and extends to the discharging station. The cartridge is arranged on the conveying mechanism and can move along the conveying mechanism on the conveying mechanism. The cartridge is used for carrying the chip sample and the reagents required for detection.

[0035] Furthermore, the conveying mechanism includes continuously arranged guide rails and a first driving mechanism. The cartridge is arranged on the guide rails, and the cartridge is in transmission connection with the first driving mechanism. The cartridge can move along the guide rails under the drive of the first driving mechanism. Alternatively, the conveying mechanism includes a belt conveyor or a chain conveyor.

[0036] Furthermore, the chip cartridge is provided with a card slot and an identification mark, and the card slot is used for accommodating and restricting the chip.

[0037] Furthermore, the identification mark includes a barcode.

[0038] Further, the pipetting assembly includes a first moving platform and an injection mechanism. The injection mechanism is disposed on the first moving platform and can move on the first moving platform. The injection mechanism is used to extract the reagents required for detection and add the reagents required for detection to the chip sample located at the pipetting station.

[0039] Further, the injection mechanism can perform the extraction and injection actions by itself.

[0040] Further, the incubation assembly includes a second moving platform, a manipulator, and an incubation mechanism. The manipulator is disposed on the second moving platform. The manipulator is used to grasp and release the chip sample. The manipulator can move on the second moving platform to transfer the chip sample between the incubation mechanism and the feeding assembly. The incubation mechanism is used to provide the incubation environment required for incubating the chip sample.

[0041] Further, the cleaning assembly includes a cleaning liquid supply mechanism and a cleaning head. The cleaning head is connected to the cleaning liquid supply mechanism.

[0042] Further, the cleaning assembly further includes a third moving platform. The cleaning head is disposed on the third moving platform and can move on the third moving platform.

[0043] Further, the vision detection assembly includes an image acquisition mechanism and an image processing mechanism. The image acquisition mechanism is connected to the image processing mechanism. The image acquisition mechanism is used to acquire the image information of the chip sample. The image processing mechanism is used to process the image information acquired by the image acquisition mechanism.

[0044] On the other hand, the present invention also provides a method for automatically detecting and processing a polypeptide chip sample. The method for automatically detecting and processing a polypeptide chip sample is implemented based on the polypeptide chip sample automatic detection and processing device.

[0045] Further, the method for automatically detecting and processing a polypeptide chip sample specifically includes:

[0046] Placing the chip on a chip cartridge to form a chip sample, placing the chip sample and the reagents required for detection on the feeding assembly, and moving the chip sample between the feeding station, the pipetting station, the incubation station, the cleaning station, the vision detection station, and the discharging station through the feeding assembly;

[0047] At the pipetting station, the reagent required for detection is added to the chip by the pipetting assembly. At the incubation station, the chip sample after adding the reagent required for detection is incubated by the incubation assembly. At the cleaning station, the chip sample after incubation is cleaned by the cleaning assembly. At the visual inspection station, the image information of the chip sample after incubation is collected and processed by the graphic detection assembly, thereby completing the automatic detection and processing of the chip.

[0048] The technical solution, its implementation process and principle will be further explained below in conjunction with the drawings and specific implementation cases. Unless otherwise specified, the manipulator, drive motor, moving platform, guide rail, belt conveyor / chain conveyor, vibration heater, pipetting head, graphic analyzer, camera, controller and related numerical control programs mentioned in the embodiments of the present invention can be known to those skilled in the art, and they can all be obtained commercially. The specific structures and equipment models thereof are not limited herein.

[0049] Please refer to Figure 1 and Figure 2 , an automatic detection and processing device for polypeptide chip samples, comprising: a loading assembly 1, a pipetting assembly 2, an incubation assembly 3, a cleaning assembly 4 and a visual detection assembly 5. The pipetting assembly 2, the incubation assembly 3, the cleaning assembly 4 and the visual detection assembly 5 are respectively arranged at the pipetting station, the incubation station, the cleaning station and the visual inspection station. The loading assembly 1 runs between the loading station, the pipetting station, the incubation station, the cleaning station, the visual inspection station and the unloading station and is used to transport the chip sample and the reagent required for detection.

[0050] Specifically, please refer to Figure 3 together. The chip sample includes a chip cartridge and a chip. The chip cartridge is used to carry the chip. More specifically, the chip cartridge includes a cartridge body 61 and an identification mark 62. A card slot 63 is arranged on the selected surface of the cartridge body 61. The card slot 63 is formed by inwardly recessing from the selected surface of the cartridge body 61 along its own thickness direction. The groove wall of the card slot 63 has a stepped structure. The outer periphery of the card slot 63 has an avoidance notch or avoidance groove for taking and placing the chip. More specifically, the identification mark 62 can be recognized and is in one-to-one correspondence with the chip arranged in the card slot 63. As a preferred solution, the identification mark 62 can be a barcode or a two-dimensional code, etc.

[0051] It should be noted that the depth of the card slot 63 is greater than the thickness of the chip. When the chip is arranged in the card slot 63, the chip is completely arranged in the card slot 63, so that when adding the reagent required for detection subsequently, the reagent can be directly added into the card slot 63, thereby ensuring that the reagent can be in full contact with the chip.

[0052] Specifically, an air guide hole is further provided inside the cartridge body 61. The air guide hole communicates with the bottom of the card slot 63 and can be connected to a negative pressure generating mechanism to adsorb the chip in the card slot 63.

[0053] Specifically, please refer to Figure 1 and Figure 4 simultaneously. The feeding assembly 1 includes a conveying mechanism 11 and a cartridge 12. The conveying mechanism 12 sequentially passes through at least the pipetting station, the incubation station, the cleaning station, the visual inspection station from the feeding station and extends to the discharging station. The cartridge 12 is arranged on the conveying mechanism 11 and can move along the conveying mechanism 11 on the conveying mechanism 11. The cartridge 12 is used to carry chip samples and reagents required for detection.

[0054] Specifically, the conveying mechanism may include a continuously arranged guide rail and a first driving mechanism. The cartridge is arranged on the guide rail, and the cartridge is in transmission connection with the first driving mechanism. The cartridge can move along the guide rail under the drive of the first driving mechanism. Alternatively, the conveying mechanism includes a belt conveying mechanism or a chain conveying mechanism. It should be noted that the first driving mechanism may be a driving motor or a driving cylinder, etc. The belt conveying mechanism or the chain conveying mechanism are known to those skilled in the art and are not specifically limited herein.

[0055] Specifically, a fixing seat is further arranged on the cartridge 12. The chip cartridge is fixed on the fixing seat. The fixing seat is placed on the cartridge and can be transferred together with the chip cartridge. More specifically, the cartridge 12 is provided with a first limiting cavity for placing the fixing seat and a second limiting cavity for placing the reagents required for detection. Among them, both the first limiting cavity and the second limiting cavity are structures with open tops to facilitate the transfer of the chip cartridge / chip sample and the addition of reagents.

[0056] Specifically, the pipetting assembly 2 is arranged at the pipetting station. The pipetting assembly is at least used to add the reagents required for detection to the chip sample located at the pipetting station.

[0057] More specifically, please refer to Figure 1 and Figure 5, the pipetting assembly 2 includes a first moving platform 21 and an injection mechanism 22. The injection mechanism 22 is disposed on the first moving platform 21 and can move on the first moving platform 21. The injection mechanism 22 is used to extract the reagents required for detection and add the reagents required for detection to the chip sample located at the pipetting station. More specifically, the first moving platform 21 can be a one-dimensional or two-dimensional or three-dimensional moving platform, which mainly includes a support frame and a driving mechanism for realizing one-dimensional or two-dimensional or three-dimensional driving. It should be noted that the first moving platform 21 is an existing functional mechanism capable of realizing one-dimensional or two-dimensional or three-dimensional movement, and its specific structure and working principle will not be elaborated here. More specifically, the injection mechanism 22 can perform the extraction and injection actions by itself, and the injection mechanism 22 can be an electronic syringe or the like.

[0058] Specifically, the incubation assembly 3 is disposed at the incubation station, and the incubation assembly 3 is at least used to incubate the chip sample after adding the reagents required for detection.

[0059] More specifically, please refer to Figure 1 and Figure 6 , the incubation assembly 3 includes a second moving platform 31, a manipulator 32 and an incubation mechanism. The manipulator 32 is disposed on the second moving platform 31. The manipulator 32 is used to grasp and release the chip sample. The manipulator 32 can move on the second moving platform 31 to transfer the chip sample between the incubation mechanism and the feeding assembly. The incubation mechanism is used to provide the incubation environment required for incubating the chip sample. More specifically, the incubation mechanism can be a vibration heater or the like. It should be noted that similar to the first moving platform, the second moving platform can be a one-dimensional / two-dimensional / three-dimensional moving platform, which is mainly used to realize the one-dimensional / two-dimensional / three-dimensional movement of the manipulator 32.

[0060] Specifically, the cleaning assembly 4 is disposed at the cleaning station, and the cleaning assembly 4 is at least used to clean the chip sample that has completed incubation.

[0061] More specifically, please refer to Figure 1 and Figure 7 , the cleaning assembly 4 includes a third moving platform 41, a cleaning liquid supply mechanism and a cleaning head 42. The cleaning head 42 is connected to the cleaning liquid supply mechanism 6. The cleaning liquid supply mechanism is used to provide cleaning liquid. The cleaning liquid supply mechanism 6 can be connected to the cleaning head 42 through a pump or the like. The cleaning head 42 is disposed on the third moving platform 41 and can move on the third moving platform 41. It should be noted that similar to the first moving platform, the third moving platform can be a one-dimensional / two-dimensional / three-dimensional moving platform, which is mainly used to realize the one-dimensional / two-dimensional / three-dimensional movement of the cleaning head 42.

[0062] Specifically, the visual detection component 5 is arranged at the visual detection station. The graphic detection component 5 is at least used to obtain the image information of the chip sample after incubation and implement the function of automatic detection and scanning imaging.

[0063] More specifically, please refer to Figure 1 and Figure 8 simultaneously. The visual detection component 5 includes an image acquisition mechanism and an image processing mechanism. The image acquisition mechanism is connected to the image processing mechanism. The image acquisition mechanism is used to acquire the image information of the chip sample, and the image processing mechanism is used to process the image information acquired by the image acquisition mechanism. More specifically, the image acquisition mechanism can be a camera or the like.

[0064] Please refer to Figure 9 simultaneously. An automatic detection and processing method for a polypeptide chip sample specifically includes the following process:

[0065] An operator manually places the chip in the chip cartridge and loads the chip cartridge and the reagents required for detection into the cartridge of the loading component.

[0066] The loading component automatically moves the cartridge to the pipetting station. At the pipetting station, the pipetting component adds the reagents required for detection to the chip, thereby realizing sample addition.

[0067] After sample addition is completed, the loading component automatically moves the cartridge to the incubation station. At the incubation station, the manipulator of the incubation component transfers the chip sample to the incubation mechanism for incubation according to the test conditions.

[0068] After incubation is completed, the manipulator of the incubation component transfers the chip sample from the incubation mechanism to the cartridge of the loading component. The loading component automatically moves the cartridge to the cleaning station. At the cleaning station, the cleaning component cleans the chip sample after incubation. After cleaning, the loading component automatically moves the cartridge to the pipetting station to perform sample addition again, moves to the incubation station to perform incubation again, and moves to the cleaning station to perform cleaning again. In this way, sample addition, incubation, and cleaning are repeated until all incubations are completed.

[0069] After the last cleaning is completed, the loading component automatically moves the cartridge to the visual detection station. The graphic detection component collects and processes the image information of the chip sample after incubation and performs graphic processing.

[0070] After the detection is completed, the loading component moves the cartridge to the unloading station, and the operator manually collects the chip cartridge, thereby completing the automatic detection and processing of the chip.

[0071] Embodiment

[0072] This example illustrates an instance of using the polypeptide chip sample processing instrument and chip cartridge of the present invention for detection.

[0073] An automatic detection and processing method for a polypeptide chip sample specifically includes the following processes:

[0074] (1) Prepare the chip cartridge and reagents and wait for subsequent operations.

[0075] (2) Add buffer (usually PBST) to the card slot of the chip cartridge, mix well by shaking, and incubate at 57 °C for 20 min - 60 min to fully wet the chip surface with the buffer.

[0076] (3) Add a blocking solution (usually a protein-based reagent), mix well by shaking, and incubate at 37 °C for 15 min - 60 min to reduce interference caused by background.

[0077] (4) Add the primary antibody, mix well by shaking, incubate at 37 °C for 60 min, and perform plate washing after incubation.

[0078] (5) Add the secondary antibody, mix well by shaking, incubate at 37 °C for 60 min, and perform plate washing after incubation.

[0079] (6) Prepare the chip cartridge for automated imaging.

[0080] (7) Scan for imaging and recover the scanned chip.

[0081] Specifically, the operator manually loads the chip cartridge, chip, and reagents into the card slots of the cartridges or reagent bottles on the feeding component respectively;

[0082] The feeding component transfers the cartridge to the pipetting station where the pipetting component is located, and buffer is added to the card slot of the chip cartridge;

[0083] As the feeding component moves, the chip moves to the incubation station for incubation at 57 °C for 20 - 60 min;

[0084] After incubation, the feeding component moves to the cleaning position, and the cleaning head cleans the chip in the chip cartridge and extracts and discharges the waste liquid;

[0085] After cleaning, the feeding component continues to move to the pipetting station. After adding the blocking solution, it moves to the incubation station for incubation at 37 °C for 15 - 60 min. After incubation, it is cleaned again and the waste liquid is aspirated. The chip cartridge moves to the pipetting station again to add the primary antibody, then moves to the incubation station for incubation at 37 °C for 60 min. Subsequently, after the cleaning head cleans the chip cartridge for the third time, the feeding component continues to move to the pipetting station, adds the secondary antibody to the card slot, incubates again, and performs the last cleaning.

[0086] After the cleaning is completed, the loading component continues to move to the vision detection position, and the camera takes pictures of the chip and starts image processing. After the image processing is completed, the operator takes out the chip cartridge that has completed the detection in the chip box, and the detection process ends.

[0087] An automatic detection and processing device for polypeptide chip samples provided by the present invention can reduce human intervention, improve the efficiency and reliability of biochip detection, ensure rapid and accurate biochip detection, and is also applicable to the application scenarios in the home environment, eliminating the need for long queues in medical institutions. A series of steps such as sampling, sample processing, detection, and obtaining reports can be completed at home, with obvious advantages in terms of detection convenience and privacy.

[0088] It should be understood that the above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit and essence of the present invention should be covered within the protection scope of the present invention.

Claims

1. An automatic detection and processing device for polypeptide chip samples, characterized in that, Comprising: A chip cartridge for carrying a chip and jointly forming a chip sample with the chip; A feeding assembly for at least conveying the chip sample and the reagents required for detection between a feeding station, a pipetting station, an incubation station, a cleaning station, a vision inspection station and a discharging station; A pipetting assembly disposed at the pipetting station, the pipetting assembly being at least used for adding the reagents required for detection to the chip sample located at the pipetting station; An incubation assembly disposed at the incubation station, the incubation assembly being at least used for incubating the chip sample after adding the reagents required for detection; A cleaning assembly disposed at the cleaning station, the cleaning assembly being at least used for cleaning the chip sample that has completed incubation; A vision inspection assembly disposed at the vision inspection station, the graphic inspection assembly being at least used for obtaining image information of the chip sample that has completed incubation.

2. The polypeptide chip sample automatic detection and processing device according to claim 1, wherein: The feeding assembly includes a conveying mechanism and a cartridge. The conveying mechanism at least sequentially passes through the pipetting station, the incubation station, the cleaning station, the vision inspection station from the feeding station and extends to the discharging station. The cartridge is disposed on the conveying mechanism and can move along the conveying mechanism on the conveying mechanism. The cartridge is used for carrying the chip sample and the reagents required for detection.

3. The polypeptide chip sample automatic detection and processing device according to claim 2, wherein: The conveying mechanism includes a continuously arranged guide rail and a first driving mechanism. The cartridge is disposed on the guide rail, and the cartridge is in transmission connection with the first driving mechanism. The cartridge can move along the guide rail under the drive of the first driving mechanism. Alternatively, the conveying mechanism includes a belt conveying mechanism or a chain conveying mechanism.

4. The polypeptide chip sample automatic detection and processing device according to claim 1, wherein: The chip cartridge is provided with a card slot and an identification mark. The card slot is used for accommodating and restricting the chip; And / or, the identification mark includes a barcode.

5. The polypeptide chip sample automatic detection and processing device according to claim 1, wherein: The pipetting assembly includes a first moving platform and an injection mechanism. The injection mechanism is disposed on the first moving platform and can move on the first moving platform. The injection mechanism is used for extracting the reagents required for detection and adding the reagents required for detection to the chip sample located at the pipetting station; And / or, the injection mechanism can perform the extraction and injection actions by itself.

6. The polypeptide chip sample automatic detection and processing device according to claim 1, wherein: The incubation assembly includes a second moving platform, a manipulator and an incubation mechanism. The manipulator is disposed on the second moving platform. The manipulator is used for grasping and releasing the chip sample. The manipulator can move on the second moving platform to transfer the chip sample between the incubation mechanism and the feeding assembly. The incubation mechanism is used for providing an incubation environment required for incubating the chip sample.

7. The polypeptide chip sample automatic detection and processing device according to claim 1, characterized in that: The cleaning assembly includes a cleaning liquid supply mechanism and a cleaning head. The cleaning head is connected to the cleaning liquid supply mechanism; And / or, the cleaning assembly further includes a third moving platform. The cleaning head is disposed on the third moving platform and can move on the third moving platform.

8. The polypeptide chip sample automatic detection and processing device according to claim 1, wherein: The visual detection component includes an image acquisition mechanism and an image processing mechanism. The image acquisition mechanism is connected to the image processing mechanism. The image acquisition mechanism is used to acquire the image information of the chip sample, and the image processing mechanism is used to process the image information acquired by the image acquisition mechanism.

9. An automatic detection method for polypeptide chip samples, characterized in that: The automatic detection and processing method for the polypeptide chip sample is implemented based on the automatic detection and processing device for the polypeptide chip sample according to any one of claims 1-8.

10. The method for automatically detecting a polypeptide chip sample according to claim 9, wherein Specifically, it includes: Placing the chip on the chip cartridge to form a chip sample, placing the chip sample and the reagents required for detection on the loading component, and moving the chip sample between the loading station, the pipetting station, the incubation station, the cleaning station, the visual detection station and the unloading station through the loading component; At the pipetting station, adding the reagents required for detection to the chip with the pipetting component. At the incubation station, incubating the chip sample after adding the reagents required for detection with the incubation component. At the cleaning station, cleaning the chip sample that has completed incubation with the cleaning component. At the visual detection station, acquiring and processing the image information of the chip sample that has completed incubation with the graphic detection component, so as to complete the automatic detection and processing of the chip.

Images