A cell sorting device

Abstract

This invention discloses a cell sorting device, relating to the field of cell sorting technology. The device includes a housing with a top cover. A cylinder is internally fitted into the top cover, and a movable polygonal column is internally fitted into the cylinder. An empty box is fixed to the upper end of the polygonal column. The invention works by inserting a needle through the bottom opening into the rubber cover. Then, by activating a water pumping or water delivery mechanism, the pumping mechanism draws the supernatant cell serum from a test tube through the empty box and side opening, along with the needle. After drawing, the supernatant cell serum is introduced into the empty box through the water delivery mechanism. This allows the supernatant cell serum to flow through the side opening and needle into an empty test tube for centrifugation. This repeated operation allows the device to continuously remove the centrifuged supernatant cell serum and place it into an empty test tube for centrifugation, thus improving the device's efficiency.

Description

Technical Field

[0001] This invention relates to the field of cell sorting technology, and more specifically to a cell sorting device. Background Technology

[0002] Cell sorting is used to separate specific cell subpopulations from mixed cell samples for subsequent biochemical analysis and functional studies. Cell sorting technology ensures the acquisition of high-purity target cells through characteristic analysis, thus providing a reliable prerequisite and foundation for cell function research.

[0003] Chinese patent (application number: CN202320818955.2) discloses a cell sorting nanomagnetic bead reagent preparation device, including a preparation box, a reagent disk rotatably installed inside the preparation box, a plurality of reagent slots evenly distributed inside the reagent disk, a movable plate rotatably installed inside the preparation box, a rotating plate rotatably installed inside the preparation box, a rotating block fixedly installed at one end of the rotating plate and in movable contact with the inner wall of the movable plate, a connecting column fixedly installed at the bottom center of the reagent disk, an arc plate fixedly connected to the bottom of the connecting column, a cylinder fixedly connected to the bottom of the arc plate away from the connecting column and in movable contact with the inner wall of the movable plate. In this invention, the rotating plate drives the rotating block to rotate inside the movable plate, making the movable plate perform a fan-shaped motion. The movable plate drives the arc plate to perform a fan-shaped motion simultaneously through the cylinder, so that the connecting column drives the reagent disk to rotate back and forth, and the reagent tubes inside the reagent slots are thoroughly mixed and stirred.

[0004] The patent and existing technologies have the following technical problems in practical use:

[0005] In current cell centrifugation and sorting methods, the supernatant cell supernatant sample needs to be repeatedly removed and placed back into the centrifugation and sorting equipment. This requires operators to frequently stop and restart the centrifugation and sorting equipment, which not only increases the workload of operators but also reduces the effectiveness of the equipment. Therefore, improvements are needed. Summary of the Invention

[0006] The purpose of this invention is to provide a cell sorting device in order to solve the above problems.

[0007] To achieve the above objectives, the present invention specifically adopts the following technical solution:

[0008] A cell sorting device includes a housing with a top cover. A cylinder is fitted inside the top cover, and a movable polygonal column is fitted inside the cylinder. An empty box is fixed to the upper end of the polygonal column. A collar block is fitted inside the empty box, and a water supply mechanism and a water pumping mechanism are respectively fixedly connected to both ends of the collar block. A needle is movably fitted inside the empty box, and a sealing block for sealing the empty box is fixed to the top of the needle. A bottom opening is opened inside the cylinder below the needle, and a side opening is opened inside the needle. A centrifugally rotating insertion plate is installed inside the housing. Test tubes are placed equidistantly in a ring inside the insertion plate, and a rubber cap is installed on the upper end of each test tube below the needle. A polygonal hole is opened inside the insertion plate below the polygonal column.

[0009] The polygonal column is moved into the polygonal hole, causing the polygonal column to move the empty box downwards. This causes the rotating insertion plate to rotate through the polygonal hole and the polygonal column, causing the empty box to rotate. At the same time, the polygonal column causes the cylinder to rotate synchronously along the inner wall of the upper cover. As the polygonal column continues to move downwards, the syringe inside the empty box passes through the bottom opening and is inserted into the rubber cover. The rubber cover, through friction, causes the syringe to press the sealing block against the inner wall of the upper end of the empty box. Simultaneously, the side opening on the syringe moves into the inner cavity of the empty box. When the syringe is inserted into the upper cell serum inside the test tube, the water pumping mechanism is activated. The water pumping mechanism extracts the upper cell serum through the empty box, the side opening, and the syringe.

[0010] Furthermore, a drive motor is fixedly installed inside the housing, and a rotating rod is driven to the upper end of the drive motor. The upper end of the rotating rod passes through the housing and is fixedly connected to the bottom of the socket plate.

[0011] Furthermore, a ring is fixedly sleeved on the outer surface of the needle tube, and a rigid spring is fixedly connected to the top of the ring. The upper end of the rigid spring is fixedly connected to the bottom of the empty box.

[0012] Furthermore, the pumping mechanism includes a second fixing block fixed to the side of the upper cover, a second water pump fixedly installed at the bottom of the second fixing block, a second hose fixedly connected to the upper end of the second water pump, the other end of the second hose passing through the second fixing block and the upper cover in sequence and fixedly connected to the collar block, and a second fixing pipe fixedly connected to the left end of the second water pump.

[0013] Furthermore, the water conveying mechanism includes a first fixing block fixed to the side of the upper cover, a first water pump fixedly installed on the side of the first fixing block, a first hose fixedly connected to the upper end of the first water pump, the other end of the first hose passing through the upper cover and fixedly connected to the collar block, and a first fixing pipe fixedly connected to the side of the first water pump.

[0014] Furthermore, an electric telescopic rod is fixedly installed at the upper end of the cover, and the lower end of the electric telescopic rod is connected to the polygonal column limiting sleeve.

[0015] Furthermore, a controller is installed inside the housing, and the controller is electrically connected to the electric telescopic rod.

[0016] Furthermore, a detection camera for detecting test tubes is installed inside the housing, and the detection camera is electrically connected to the controller.

[0017] The beneficial effects of the present invention are as follows:

[0018] 1. This invention involves inserting a needle through the bottom opening into the rubber cap, and then activating a water pumping or water delivery mechanism. This allows the water pumping mechanism to extract the supernatant cell serum from the test tube through the empty chamber and side opening, along with the needle. After extraction, the supernatant cell serum is introduced into the empty chamber through the water delivery mechanism. This allows the supernatant cell serum to flow into an empty test tube through the side opening and needle for centrifugation. By repeating this operation, the device can remove the supernatant cell serum after centrifugation without stopping the machine and place it into an empty test tube for centrifugation, thereby improving the effectiveness of the device.

[0019] 2. This invention inserts a polygonal column into a polygonal hole, causing a rotating insertion plate to rotate through the polygonal hole and the polygonal column, thereby causing the empty box and cylinder to rotate. This causes the empty box to carry a syringe through the bottom opening and into the rubber cap. The rubber cap then pushes the side opening on the syringe into the inner cavity of the empty box until the syringe is inserted into the upper layer of cell supernatant inside the test tube. Then, by activating the water delivery mechanism, the cell supernatant sample is introduced into the empty box, allowing the cell supernatant sample to flow into the empty test tube through the side opening and the syringe. This achieves the purpose of adding cell supernatant sample for centrifugation and sorting without stopping the machine.

[0020] 3. This invention uses a detection camera to detect the cell stock solution sample inside the test tube, and the detection camera transmits the detection data to the controller, which then activates the electric telescopic rod. The electric telescopic rod drives the needle through the bottom opening and inserts it into the test tube, thereby achieving the purpose of timely extraction of the upper layer of cell clear solution sample inside the test tube, thus improving the efficiency of cell sample sorting of the device. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the present invention;

[0022] Figure 2 This is a schematic diagram of the back of the invention;

[0023] Figure 3 This is a front cross-sectional view of the present invention;

[0024] Figure 4This is a side sectional view of the housing of the present invention;

[0025] Figure 5 This is a schematic diagram of the needle and rubber cap of the present invention;

[0026] Figure 6 This is the present invention. Figure 1 A magnified view of part A;

[0027] Figure 7 This is the present invention. Figure 2 A magnified view of part B;

[0028] Figure 8 This is the present invention. Figure 3 A magnified view of part C.

[0029] Reference numerals: 1. Box body; 2. Top cover; 3. Cylinder; 4. Polygonal column; 5. Empty box; 6. Sealing block; 7. Needle tube; 8. Ring; 9. Rigid spring; 10. Side opening; 11. Bottom opening; 12. Insertion plate; 13. Test tube; 14. Rubber cap; 15. Water delivery mechanism; 1501. First fixing block; 1502. First water pump; 1503. First hose; 1504. First fixing pipe; 16. Pumping mechanism; 1601. Second fixing block; 1602. Second water pump; 1603. Second hose; 1604. Second fixing pipe; 17. Electric telescopic rod; 18. Detection camera; 19. Controller; 20. Drive motor; 21. Rotating rod; 22. Polygonal hole; 23. Collar block. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0031] Example 1, as Figures 1-8 As shown, a cell sorting device includes a housing 1, a top cover 2 installed on the housing 1, a cylinder 3 fitted inside the top cover 2, a movable polygonal column 4 fitted inside the cylinder 3, an empty box 5 fixed at the upper end of the polygonal column 4, a collar block 23 fitted inside the empty box 5, a water conveying mechanism 15 and a water pumping mechanism 16 respectively fixedly connected at both ends of the collar block 23, a needle tube 7 movably fitted inside the empty box 5, a sealing block 6 for sealing the empty box 5 fixed at the top of the needle tube 7, a bottom opening 11 located below the needle tube 7 inside the cylinder 3, a side opening 10 inside the needle tube 7, a centrifugally rotating insertion plate 12 installed inside the housing 1, test tubes 13 are placed equidistantly in a ring inside the insertion plate 12, a rubber cap 14 located below the needle tube 7 is installed at the upper end of the test tubes 13, and a polygonal hole 22 located below the polygonal column 4 inside the insertion plate 12.

[0032] When it is necessary to remove the supernatant cell solution after centrifugation in test tube 13, the polygonal column 4 is moved into the polygonal orifice 22, causing the polygonal column 4 to move the empty box 5 downwards. This allows the rotating insertion plate 12 to rotate through the polygonal orifice 22 and the polygonal column 4, causing the empty box 5 to rotate. Simultaneously, the polygonal column 4 causes the cylinder 3 to rotate synchronously along the inner wall of the upper cover 2. As the polygonal column 4 continues to move downwards, the syringe 7 inside the empty box 5 passes through the bottom opening 11 and inserts into the rubber cap 14. Due to the friction between the syringe 7 and the rubber cap 14, the rubber cap 14, through the syringe 7, presses the sealing block 6 against the inner wall of the upper end of the empty box 5. Simultaneously, the side opening 10 on the syringe 7 moves into the inner cavity of the empty box 5. Then, when the syringe 7 is inserted into the supernatant cell solution inside test tube 13, the pumping mechanism 16 is activated, thereby... The pumping mechanism 16 draws the supernatant cell solution from the test tube 13 through the empty box 5, side opening 10, and syringe 7. After drawing, the polygonal column 4 moves upward, causing it to disengage from the polygonal orifice 22. Simultaneously, the syringe 7 and rubber cap 14 pull the sealing block 6 to close the empty box 5 through friction until the syringe 7 disengages from the rubber cap 14. Then, the supernatant cell solution is introduced into the empty box 5 through the water delivery mechanism 15. Subsequently, by moving the polygonal column 4 downward again, the empty box 5 drives the syringe 7 to insert into an empty test tube 13. This allows the supernatant cell solution inside the empty box 5 to flow into the empty test tube 13 through the side opening 10 and syringe 7 for centrifugation. This repeated operation allows the device to remove the centrifuged supernatant cell solution without stopping the machine and place it into an empty test tube 13 for centrifugation, thereby improving the device's performance.

[0033] Furthermore, a drive motor 20 is fixedly installed inside the housing 1. A rotating rod 21 is connected to the upper end of the drive motor 20. The upper end of the rotating rod 21 passes through the housing 1 and is fixedly connected to the bottom of the socket plate 12.

[0034] By starting the drive motor 20, the rotating rod 21 drives the insertion plate 12 to rotate along the inner wall of the box 1, which in turn drives the test tube 13 to rotate, so that the cell samples inside the test tube 13 can be centrifuged and sorted, thereby achieving the purpose of centrifuging and sorting cell samples by rotating the insertion plate 12.

[0035] Furthermore, a ring 8 is fixedly sleeved on the outer surface of the needle tube 7, and a rigid spring 9 is fixedly connected to the top of the ring 8. The upper end of the rigid spring 9 is fixedly connected to the bottom of the empty box 5.

[0036] When the syringe 7 is completely detached from the rubber cap 14, due to the design of the ring 8 and the rigid spring 9, the rigid spring 9 can squeeze the syringe 7 downward through the ring 8, which in turn causes the syringe 7 to drive the sealing block 6 to fit more tightly against the inner wall of the empty box 5, making it less likely for the liquid inside the empty box 5 to flow out.

[0037] Example 2, as Figures 3-7 As shown, the pumping mechanism 16 includes a second fixing block 1601 fixed to the side of the upper cover 2. A second water pump 1602 is fixedly installed at the bottom of the second fixing block 1601. A second hose 1603 is fixedly connected to the upper end of the second water pump 1602. The other end of the second hose 1603 passes through the second fixing block 1601 and the upper cover 2 in sequence and is fixedly connected to the collar block 23. A second fixing pipe 1604 is fixedly connected to the left end of the second water pump 1602.

[0038] When the needle 7 is inserted into the test tube 13, the second water pump 1602 is activated, so that the second tubing 1603 can draw the upper cell clear fluid sample inside the test tube 13 through the empty box 5 and the side port 10 and the needle 7, and discharge the sample into the external test cup through the second fixed tube 1604.

[0039] The water conveying mechanism 15 includes a first fixing block 1501 fixed to the side of the upper cover 2. A first water pump 1502 is fixedly installed on the side of the first fixing block 1501. A first hose 1503 is fixedly connected to the upper end of the first water pump 1502. The other end of the first hose 1503 passes through the upper cover 2 and is fixedly connected to the collar block 23. A first fixing pipe 1504 is fixedly connected to the side of the first water pump 1502.

[0040] When it is necessary to introduce the supernatant cell culture sample or cell sample from the test cup into the empty test tube 13, the polygonal column 4 is moved downwards and inserted into the polygonal hole 22. This causes the rotating insertion plate 12 to rotate through the polygonal hole 22 and the polygonal column 4, thereby causing the empty box 5 and the cylinder 3 to rotate. As the polygonal column 4 continues to move downwards, the needle 7 inside the empty box 5 passes through the bottom opening 11 and is inserted into the rubber cap 14. At this time, the rubber cap 14 will press the sealing block 6 against the inner wall of the upper end of the empty box 5 through the needle 7, and cause the side opening 10 on the needle 7 to move into the inner cavity of the empty box 5. As the polygonal column 4 continues to move downwards, the needle... The tube 7 is inserted into the upper cell solution inside the test tube 13. Then, by starting the first water pump 1502, the first fixed tube 1504 can draw the cell solution sample or cell stock sample from the test cup. Then, the first flexible tube 1503 introduces the upper cell solution sample or cell stock sample into the empty box 5. Thus, the upper cell solution sample or cell stock sample flows into the empty test tube 13 or the test tube 13 with precipitate through the side port 10 and the needle tube 7. In this way, the upper cell solution sample and the cell stock sample can be centrifuged and sorted separately, thereby achieving the purpose of adding cell stock sample for centrifugation and sorting without stopping the machine.

[0041] Example 3, as Figure 1 and Figure 3 and Figure 4 As shown, an electric telescopic rod 17 is fixedly installed on the upper end of the cover 2, and the lower end of the electric telescopic rod 17 is connected to the polygonal column 4 for limiting.

[0042] By activating the electric telescopic rod 17, the electric telescopic rod 17 drives the polygonal column 4 to move downward, thereby allowing the polygonal column 4 to drive the needle 7 to be inserted into the test tube 13 through the empty box 5 to extract the upper cell clear fluid sample.

[0043] The housing 1 is equipped with a controller 19, which is electrically connected to the electric telescopic rod 17.

[0044] Through the design of the controller 19 and the electric telescopic rod 17, the controller 19 can control the electric telescopic rod 17 to start, thereby driving the polygonal column 4 to move up or down.

[0045] Inside the housing 1 is a detection camera 18 with a test tube 13, and the detection camera 18 is electrically connected to the controller 19.

[0046] When the detection camera 18 detects that the cell culture sample inside the test tube 13 has been centrifuged into a supernatant of cell supernatant and precipitate, the detection camera 18 transmits a signal to the controller 19. This causes the controller 19 to activate the electric telescopic rod 17, which in turn drives the polygonal column 4 downwards to insert into the polygonal hole 22. Simultaneously, the drive motor 20, through the rotating rod 21, rotates the insertion plate 12, causing it to rotate through the polygonal hole 22 and the polygonal column 4, thus rotating the empty box 5 and the cylinder 3. This, in turn, causes the polygonal column 4 to rotate through the empty box 5. The syringe 7 passes through the bottom opening 11 and is inserted into the rubber cap 14, causing the rubber cap 14 to push the sealing block 6 upward through the syringe 7. This causes the side opening 10 on the syringe 7 to move into the inner cavity of the empty box 5 until the syringe 7 is inserted into the upper layer of cell solution inside the test tube 13. At this point, the second water pump 1602 is activated, allowing the second tubing 1603 to draw the upper layer of cell solution sample from the test tube 13 through the empty box 5 and the side opening 10, and then discharge the sample into the external detection cup through the second fixing tube 1604. When the detection camera 18 detects the upper layer of cell solution inside the test tube 13... After sample extraction, the detection signal is transmitted to the controller 19, which in turn activates the electric telescopic rod 17. This drives the polygonal column 4 upwards, causing the empty box 5 to disengage the syringe 7 from the rubber cap 14 via the sealing block 6. The controller 19 then detects the position of the empty test tube 13 within the insertion plate 12 via the detection camera 18, and uses this information to control the insertion of the polygonal column 4 into the polygonal hole 22. At this point, the syringe 7 is positioned above the empty test tube 13, allowing it to pass through the rubber cap 14 and be inserted into the empty test tube 13. The operator then performs the sampling... The test cup is placed below the first fixed tube 1504, allowing the first fixed tube 1504 to draw liquid from the test cup. Then, by activating the first water pump 1502, the first fixed tube 1504 draws the upper layer of cell supernatant sample from the test cup, and the first flexible tube 1503 introduces the upper layer of cell supernatant sample into the empty box 5. The upper layer of cell supernatant sample then flows into the empty test tube 13 through the side port 10 and the syringe 7 for centrifugation and sorting, thereby achieving the purpose of timely extraction of the upper layer of cell supernatant sample from the test tube 13, thus improving the efficiency of cell sample sorting by the device.

[0047] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell sorting device, comprising a housing (1), characterized in that, A top cover (2) is installed on the box body (1). A cylinder (3) is fitted inside the top cover (2). A lifting polygonal column (4) is movably fitted inside the cylinder (3). An empty box (5) is fixed at the upper end of the polygonal column (4). A collar block (23) is fitted inside the empty box (5). A water conveying mechanism (15) and a water pumping mechanism (16) are fixedly connected at both ends of the collar block (23). A syringe (7) is movably fitted inside the empty box (5). A sealing device is fixed at the top of the syringe (7). The enclosed block (6) of the empty box (5), the cylinder (3) has a bottom opening (11) located below the needle tube (7) inside, the needle tube (7) has a side opening (10) inside, the box body (1) is equipped with a centrifugally rotating insertion plate (12) inside, the insertion plate (12) has test tubes (13) placed in a ring at equal intervals inside, the test tubes (13) have a rubber cap (14) located below the needle tube (7) at the upper end, and the insertion plate (12) has a polygonal hole (22) located below the polygonal column (4) inside. Move the polygonal column (4) into the polygonal hole (22), causing the polygonal column (4) to move the empty box (5) downwards. This causes the rotating insertion plate (12) to rotate through the polygonal hole (22) and the polygonal column (4), causing the empty box (5) to rotate. At the same time, the polygonal column (4) causes the cylinder (3) to rotate synchronously along the inner wall of the upper cover (2). As the polygonal column (4) continues to move downwards, the needle (7) inside the empty box (5) passes through the bottom opening (11) and is inserted. Inside the rubber cap (14), the rubber cap (14) drives the syringe (7) to press the sealing block (6) against the inner wall of the upper end of the empty box (5) through friction. At the same time, the side port (10) on the syringe (7) moves into the inner cavity of the empty box (5). When the syringe (7) is inserted into the upper cell clear liquid inside the test tube (13), the water pumping mechanism (16) is activated. The water pumping mechanism (16) extracts the upper cell clear liquid through the empty box (5) and the side port (10) and the syringe (7).

2. The cell sorting device according to claim 1, characterized in that, A drive motor (20) is fixedly installed inside the housing (1). A rotating rod (21) is connected to the upper end of the drive motor (20). The upper end of the rotating rod (21) passes through the housing (1) and is fixedly connected to the bottom of the socket plate (12).

3. The cell sorting device according to claim 1, characterized in that, A ring (8) is fixedly sleeved on the outer surface of the needle tube (7), and a rigid spring (9) is fixedly connected to the top of the ring (8). The upper end of the rigid spring (9) is fixedly connected to the bottom of the empty box (5).

4. The cell sorting device according to claim 1, characterized in that, The pumping mechanism (16) includes a second fixing block (1601) fixed to the side of the upper cover (2), a second water pump (1602) fixedly installed at the bottom of the second fixing block (1601), a second hose (1603) fixedly connected to the upper end of the second water pump (1602), the other end of the second hose (1603) passing through the second fixing block (1601) and the upper cover (2) in sequence and fixedly connected to the collar block (23), and a second fixing pipe (1604) fixedly connected to the left end of the second water pump (1602).

5. The cell sorting device according to claim 1, characterized in that, The water conveying mechanism (15) includes a first fixing block (1501) fixed to the side of the upper cover (2), a first water pump (1502) fixedly installed on the side of the first fixing block (1501), a first hose (1503) fixedly connected to the upper end of the first water pump (1502), the other end of the first hose (1503) passing through the upper cover (2) and fixedly connected to the collar block (23), and a first fixing pipe (1504) fixedly connected to the side of the first water pump (1502).

6. The cell sorting device according to claim 1, characterized in that, An electric telescopic rod (17) is fixedly installed on the upper end of the cover (2), and the lower end of the electric telescopic rod (17) is connected to the polygonal column (4) for limiting.

7. The cell sorting device according to claim 6, characterized in that, The housing (1) is equipped with a controller (19), which is electrically connected to the electric telescopic rod (17).

8. The cell sorting device according to claim 7, characterized in that, The housing (1) is equipped with a detection camera (18) for the test tube (13), and the detection camera (18) is electrically connected to the controller (19).

Images