Cell filtering, sorting and enriching smear device

Abstract

The invention belongs to the technical field of cell smears, and discloses a cell filtering, sorting and enriching smear device which comprises an accommodating and resuspending mechanism, a filtering mechanism and a smear mechanism. The accommodating and resuspending mechanism comprises a sample accommodating assembly and a resuspending assembly, the sample accommodating assembly and the resuspending assembly can be coaxially and integrally connected or mutually independent, the sample accommodating assembly accommodates a liquid biological sample, and the resuspending assembly accommodates resuspending liquid; the filtering mechanism is detachably connected to the sample containing assembly and further detachably connected to the resuspension assembly, a filtering membrane is arranged in the filtering mechanism, and a liquid biological sample is filtered and enriched through the filtering membrane and then is resuspended to obtain a cell suspension; the smear mechanism is connected to the centrifugal smear machine and can bear a glass slide and contain the filtering mechanism, and the cell suspension can flow to the glass slide from the filtering mechanism. The cell filtering, sorting, enriching and smearing device can realize cell separation, enrichment and smearing processes, prevent cell loss, improve the smearing quality and shorten the smearing time.

Description

Technical Field

[0001] The present invention relates to the technical field of cell smear techniques, and particularly to a cell filtration, sorting, enrichment and smear device. Background Art

[0002] Nucleated cells (such as malignant tumor cells, hematological disease cells, cardiovascular exfoliated cells, maternal blood fetal cells and microbial cells, etc.) in body fluids (blood, urine, pleural effusion, ascites, cerebrospinal fluid, joint effusion, saliva, sputum, secretions, throat and cervical swabs, etc.) contain rare cells such as circulating tumor cells and immune cells, and the above rare cells all have important clinical value in medical treatment. At present, the detection methods of rare cells in blood can be roughly divided into two categories: one is the detection based on the surface molecular markers of rare cells, and the other is the separation and detection based on the physical properties of rare cells themselves.

[0003] For the separation of rare cells, it is first necessary to separate the rare cells in the blood, and then coat the rare cells on a glass slide to achieve smearing. However, at present, some cells are easily lost during the separation and smearing of rare cells, and there are problems such as unstable smear quality and the need to consume a large amount of time and labor for smearing.

[0004] Therefore, there is an urgent need for a cell filtration, sorting, enrichment and smear device to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a cell filtration, sorting, enrichment and smear device, which can realize the processes of cell separation, enrichment and smearing, prevent cell loss, improve smear quality and reduce smear time.

[0006] To achieve this purpose, the present invention adopts the following technical solutions:

[0007] A cell filtration, sorting, enrichment and smear device, comprising:

[0008] A containing and resuspending mechanism, the containing and resuspending mechanism includes a sample containing component and a resuspending component, the sample containing component and the resuspending component can be coaxially and integrally connected or independent of each other, the sample containing component contains a liquid biological sample, and the resuspending component contains a resuspension liquid;

[0009] A filtering mechanism, the filtering mechanism is detachably connected to the sample containing component and can also be detachably connected to the resuspending component, a filter membrane is provided in the filtering mechanism, the liquid biological sample is filtered and enriched by the filter membrane and then resuspended by the resuspension liquid to obtain a cell suspension;

[0010] A smearing mechanism, which is connected to a centrifugal smearing machine, can carry a glass slide and accommodate the filtering mechanism, and the cell suspension can flow from the filtering mechanism to the glass slide.

[0011] As a preferred solution of the cell filtration, sorting, enrichment and smearing device provided by the present invention, a first clamping structure is provided at the end of the sample accommodating component, and the first clamping structure can be fixed to the filter; and / or,

[0012] A second clamping structure is provided at the end of the resuspension component, and the second clamping structure can be fixed to the filter.

[0013] As a preferred solution of the cell filtration, sorting, enrichment and smearing device provided by the present invention, the filtering mechanism is screwed to the sample accommodating component or the resuspension component.

[0014] As a preferred solution of the cell filtration, sorting, enrichment and smearing device provided by the present invention, a first annular groove is provided on the outer peripheral side of the sample accommodating component, and a first sealing ring is provided in the first annular groove. The first sealing ring can form a seal between the outer periphery of the sample accommodating component and the inner wall of the filter.

[0015] As a preferred solution of the cell filtration, sorting, enrichment and smearing device provided by the present invention, a through port is provided on the side of the filtering mechanism, and the through port is configured to allow the cell suspension passing through the filter membrane to flow out;

[0016] A first stop cylinder is provided at the end of the sample accommodating component, and the first stop cylinder can be inserted into the filtering mechanism to block the through port; and / or,

[0017] A second stop cylinder is provided at the end of the resuspension component, and the second stop cylinder can be inserted into the filtering mechanism to block the through port.

[0018] As a preferred solution of the cell filtration, sorting, enrichment and smearing device provided by the present invention, an annular boss is provided on the inner wall of the filtering mechanism, and the annular boss is used to position the filter membrane.

[0019] As a preferred solution of the cell filtration, sorting, enrichment and smearing device provided by the present invention, the smearing mechanism includes an installation cylinder and a slide component which are connected in communication. The slide component can position the glass slide and is connected to the centrifugal smearing machine. The filtering mechanism can be accommodated in the installation cylinder, the outlet of the filtering mechanism can be communicated with the slide component, and the cell suspension can flow to the glass slide.

[0020] As a preferred embodiment of the cell filtration, sorting, enrichment and smear device provided by the present invention, an installation groove is provided at the bottom of the installation cylinder, and a magnet is arranged in the installation groove. The magnet is configured to adsorb the magnetic material added to the liquid biological sample, and the magnetic material can bind to the non-target cells in the liquid biological sample.

[0021] As a preferred embodiment of the cell filtration, sorting, enrichment and smear device provided by the present invention, a positioning convex block is provided at the bottom of the installation cylinder, and a positioning groove is provided at the bottom of the filtration mechanism. The positioning convex block can be inserted into the positioning groove so that the outlet of the filtration mechanism is directly opposite to the inlet of the slide assembly.

[0022] As a preferred embodiment of the cell filtration, sorting, enrichment and smear device provided by the present invention, the slide assembly includes a bearing plate and a clamping plate connected movably. The installation cylinder is connected to the clamping plate, and a receiving groove is formed between the clamping plate and the bearing plate. The receiving groove accommodates the slide.

[0023] Advantages of the present invention:

[0024] The cell filtration, sorting, enrichment and smear device provided by the present invention includes a receiving and resuspending mechanism, a filtration mechanism and a smear mechanism. The receiving and resuspending mechanism can be connected to a filter, and includes a sample receiving assembly and a resuspending assembly. The sample receiving assembly and the resuspending assembly can be coaxially and integrally connected or independent of each other. The sample receiving assembly contains a liquid biological sample, and the resuspending assembly contains a resuspension solution. The filtration mechanism is detachably connected to the sample receiving assembly and can also be detachably connected to the resuspending assembly. A filter membrane is provided in the filtration mechanism. The liquid biological sample is filtered and enriched through the filter membrane and then resuspended by the resuspension solution to obtain a cell suspension. That is to say, through the receiving and resuspending mechanism and the filtration mechanism, in cooperation with the filter, the whole process of filtration, enrichment and resuspension of the liquid biological sample can be realized. The smear mechanism is connected to a centrifugal smearer, can carry a slide and accommodate the filtration mechanism, and the cell suspension can flow from the filtration mechanism to the slide. That is to say, all or part of the receiving and resuspending mechanism, the filtration mechanism and the smear mechanism cooperate with the centrifugal smearer, and the cell suspension can be directly transferred onto the slide, preventing cell loss, improving the smear quality and shortening the smear time. Description of the Drawings

[0025] Figure 1 is a schematic diagram of the cell filtration, sorting, enrichment and smear device provided in Embodiment 1 of the present invention;

[0026] Figure 2 is a cross-sectional view of the sample receiving assembly provided in Embodiment 1 of the present invention;

[0027] Figure 3 is a cross-sectional view of the resuspending assembly provided in Embodiment 1 of the present invention;

[0028] Figure 4 is a schematic diagram of the structure of the filtering mechanism provided in the first embodiment of the present invention Figure 1 ;

[0029] Figure 5 is a schematic diagram of the structure of the filtering mechanism provided in the first embodiment of the present invention Figure 2 ;

[0030] Figure 6 is a schematic diagram of the structure of a smear mechanism provided in the first embodiment of the present invention (top view angle);

[0031] Figure 7 is a schematic diagram of the structure of another smear mechanism provided in the first embodiment of the present invention (front view angle);

[0032] Figure 8 is a schematic diagram of the structure of another smear mechanism provided in the first embodiment of the present invention (top view angle);

[0033] Figure 9 is a schematic diagram of the cell filtering, sorting, enriching and smearing device provided in the second embodiment of the present invention;

[0034] Figure 10 is a cross-sectional view of the accommodating and resuspending mechanism provided in the second embodiment of the present invention.

[0035] In the figure:

[0036] 100, accommodating and resuspending mechanism; 110, sample accommodating component; 111, first clamping flap structure; 112, first annular groove; 113, first stop cylinder; 114, first thread section; 120, resuspending component; 121, second clamping flap structure; 122, second stop cylinder; 123, second thread section;

[0037] 200, filtering mechanism; 210, through port; 220, annular boss; 230, second annular groove; 240, connecting thread; 250, positioning groove;

[0038] 300, smear mechanism; 310, mounting cylinder; 311, positioning convex block; 312, mounting groove; 320, slide carrier component; 321, bearing plate; 322, clamping plate; 323, buckle component; 330, communicating pipe. Detailed implementation manners

[0039] The present invention will be further described in detail below with reference to the drawings and embodiments. It can be understood that the specific embodiments described herein are only used to explain the present invention, rather than limiting the present invention. Additionally, it should be noted that for the sake of description, only parts related to the present invention are shown in the drawings, rather than all the structures.

[0040] In the description of the present invention, unless otherwise clearly defined and limited, the terms "connected", "connected to", and "fixed" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral body; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0041] In the present invention, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features therebetween. Moreover, the first feature being "above", "over", and "on top of" the second feature includes the first feature being directly above and obliquely above the second feature, or merely indicating that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "beneath", and "underneath" the second feature includes the first feature being directly below and obliquely below the second feature, or merely indicating that the horizontal height of the first feature is lower than that of the second feature.

[0042] In the description of this embodiment, the orientation or positional relationships such as "above", "below", "right", and "left" are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of description and simplifying the operation, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention. In addition, the terms "first" and "second" are only used for distinction in description and have no special meanings.

[0043] Embodiment 1

[0044] Figure 1 The schematic diagram showing the cell filtration, sorting, enrichment, and smear device provided by Embodiment 1 of the present invention is shown. Refer to Figure 1 , this embodiment provides a cell filtration, sorting, enrichment, and smear device. The cell filtration, sorting, enrichment, and smear device includes a containing and resuspending mechanism 100, a filtration mechanism 200, and a smear mechanism 300. A filter membrane is provided in the filtration mechanism 200. The containing and resuspending mechanism 100 can be connected to the filtration mechanism 200 and cooperate with the filter to achieve the filtration, enrichment, and resuspension of the liquid biological sample, and after forming a cell suspension, it is smeared by the smear mechanism 300 in cooperation with a centrifugal smearer.

[0045] Specifically, the accommodation and resuspension mechanism 100 includes a sample accommodation component 110 and a resuspension component 120. The sample accommodation component 110 and the resuspension component 120 are independent of each other. The sample accommodation component 110 contains a liquid biological sample, and the resuspension component 120 contains a resuspension liquid. The sample accommodation component 110 and the resuspension component 120 can be respectively connected to the filtering mechanism 200 to cooperate with the filter to achieve the processes of filtration enrichment and resuspension to form a cell suspension.

[0046] More specifically, in this embodiment, the filter is a sealed syringe filter in existing filtering equipment, or can be a device such as a centrifuge tube with a closed bottom, and this embodiment does not limit this here. When in use, the sample accommodation component 110 is connected to the filtering mechanism 200 and inserted into the syringe of the filter, and it is ensured that the sample accommodation component 110 is in close contact with the inner side wall of the syringe. The upper end of the sample accommodation component 110 is fixed to the push-pull device of the filtering equipment, and the push-pull device can move relative to the syringe along the axial direction of the syringe to push or pull the sample accommodation component 110, so that the cells in the liquid biological sample are enriched in the filtering mechanism 200 under the above-mentioned pushing and pulling actions. Then, the sample accommodation component 110 and the filtering mechanism 200 are disassembled, the resuspension component 120 and the filtering mechanism 200 are connected, and the resuspension component 120 and the filtering mechanism 200 are pushed along the axial direction into the component of the smear mechanism 300 for accommodating the filtering mechanism 200, and during this process, it is ensured that the filtering mechanism 200 is in close contact with the inner wall of the component of the smear mechanism 300 for accommodating the filtering mechanism 200. During the above-mentioned pushing process, the cells enriched in the filtering mechanism 200 and the resuspension liquid in the resuspension component 120 form a cell suspension. In this embodiment, the liquid biological sample can be body fluids such as blood and saliva, or can be a microbial suspension, etc., and this embodiment does not limit the specific type of the liquid biological sample.

[0047] Figure 2 A cross-sectional view of the sample accommodation component provided in the first embodiment of the present invention is shown. Figure 3 A cross-sectional view of the resuspension component provided in the first embodiment of the present invention is shown. Referring to Figure 2 and Figure 3 , a first flap structure 111 is provided at the end of the sample accommodation component 110, and the first flap structure 111 can be fixed to the push-pull device of the filter. And a second flap structure 121 is provided at the end of the resuspension component 120, and the second flap structure 121 can also be fixed to the push-pull device of the filter.

[0048] Specifically, the filtering mechanism 200 is screwed to the sample accommodating assembly 110 or the resuspension assembly 120. In this embodiment, the sample accommodating assembly 110 is provided with a first threaded section 114. The resuspension assembly 120 is provided with a second threaded section 123. The inner wall at the opening of the filtering mechanism 200 is provided with connecting threads 240. Both the first threaded section 114 and the second threaded section 123 are matched with the connecting threads 240.

[0049] More specifically, a first annular groove 112 is annularly provided on the outer peripheral side of the sample accommodating assembly 110. A first sealing ring is arranged in the first annular groove 112. The first sealing ring can form a seal between the outer periphery of the sample accommodating assembly 110 and the inner wall of the syringe of the filter, ensuring that the two are closely attached. In this embodiment, the first sealing ring can be selected as a silicone sealing ring.

[0050] Figure 4 Schematic structural diagram of the filtering mechanism provided in the first embodiment of the present invention Figure 1 , Figure 5 Schematic structural diagram of the filtering mechanism provided in the first embodiment of the present invention Figure 2 . Refer to Figure 4 and Figure 5 , a second annular groove 230 is annularly provided on the outer peripheral side of the filtering mechanism 200. A second sealing ring is arranged in the second annular groove 230. The second sealing ring can form a seal between the outer peripheral side of the filtering mechanism 200 and the inner wall of the component of the smear mechanism 300 for accommodating the filtering mechanism 200. In this embodiment, the second sealing ring can also be selected as a silicone sealing ring.

[0051] Figure 6 Schematic structural diagram (top view angle) of a smear mechanism provided in the first embodiment of the present invention. Refer to Figure 1 and Figure 6 , the smear mechanism 300 includes a mounting cylinder 310, a slide component 320 and a communication pipe 330. The communication pipe 330 communicates between the mounting cylinder 310 and the slide component 320. The slide component 320 positions the slide and is connected to the centrifugal smearer. The mounting cylinder 310 can accommodate the resuspension mechanism 100 and the filtering mechanism 200. The second sealing ring provided on the outer peripheral side of the filtering mechanism 200 can form a seal with the inner wall of the mounting cylinder 310. The outlet of the filtering mechanism 200 communicates with the slide component 320, and the cell suspension can flow to the slide.

[0052] Specifically, a through port 210 is opened on the side of the filtering mechanism 200. The through port 210 is configured to allow the cell suspension to flow out, and the through port 210 is selectively communicated with the slide component 320. That is to say, the through port 210 is the outlet of the filtering mechanism 200. The communication position between the communication pipe 330 and the side of the mounting cylinder 310 is arranged opposite to the through port 210.

[0053] More specifically, the end of the sample holding component 110 is provided with a first stopper 113, which can be inserted into the filtering mechanism 200 to block the through-port 210 during the filtering process to prevent the liquid biological sample from flowing out of the through-port 210. The end of the resuspension component 120 is provided with a second stopper 122, which can be inserted into the filtering mechanism 200 to block the through-port 210 to prevent the resuspension liquid from flowing out of the through-port 210 during the resuspension process.

[0054] More specifically, the inner wall of the filter mechanism 200 is provided with an annular boss 220, and the annular boss 220 is used to position the filter membrane. In order to ensure the stable installation of the filter membrane, a gasket or a pressure ring is used to position and press the filter membrane so that it is stably installed on the annular boss 220. The above-mentioned through port 210 is arranged above the annular boss 220. That is, the cells enriched on the filter membrane can be mixed by the resuspension of the filter membrane to form a cell suspension, and the resuspension component 120 and the filter mechanism 200 are installed in the installation cylinder 310, and the resuspension component 120 and the filter mechanism 200 are loosened, and the through port 210 is opened, and then the cell suspension can flow out to the connecting pipe 330 through the through port 210.

[0055] Continue to refer to Figure 1 and Figure 6 The slide assembly 320 includes a movably connected carrying plate 321 and a clamping plate 322, the mounting tube 310 is connected to the clamping plate 322, and a receiving groove can be formed between the clamping plate 322 and the carrying plate 321 to accommodate the slide.

[0056] Specifically, the rotating connection end of the clamping plate 322 is rotatably connected to the carrying plate 321 by a hinged manner, and a buckle 323 is provided on the carrying plate 321, and the buckle 323 is detachably buckled to the free end of the clamping plate 322. When the clamping plate 322 is rotatably buckled to the carrying plate 321, the buckle 323 is buckled to the clamping plate 322 to ensure the reliability of the installation of the slide in the receiving groove.

[0057] Optionally, in other embodiments, the clamping plate 322 is fixedly connected to the carrying plate 321 , and a gap is left between the clamping plate 322 and the carrying plate 321 for inserting a glass slide.

[0058] Figure 7 FIG. 2 is a schematic diagram showing the structure of another smear mechanism provided by Embodiment 1 of the present invention (from a main viewing angle). Figure 7 This embodiment also provides another slide assembly 320. The slide assembly 320 directly uses the clamping tool provided on the centrifugal smear machine to clamp and fix the slide, which is simpler to operate.

[0059] Figure 8Schematic diagram (top view angle) showing another smear mechanism provided in the first embodiment of the present invention. Refer to Figure 5 and Figure 8 , preferably, a positioning projection 311 is provided at the bottom of the mounting cylinder 310, and a positioning groove 250 is correspondingly provided at the bottom of the filtering mechanism 200. The positioning projection 311 can be inserted into the positioning groove 250 to fix the installation angle of the filtering mechanism 200 in the mounting cylinder 310. At this time, the accurate alignment of the through port 210 and the connecting pipe 330 can be ensured, and the problem of cell loss during the centrifugal smear process can be avoided.

[0060] , preferably, a third sealing ring is provided around the outside of the through port 210 to ensure reliable sealing between the through port 210 and the connecting pipe 330 when the filtering mechanism 200 is received in the mounting cylinder 310 and prevent leakage.

[0061] , preferably, an installation groove 312 is provided at the bottom of the mounting cylinder 310, and a magnet is provided in the installation groove 312. If a purification process is required after the resuspension process, an antibody-magnetic bead complex is added to the resuspension solution at the same time. After the resuspension process, let it stand for a period of time so that the non-target cells in the cell suspension that can bind to the antibody-magnetic bead complex are adsorbed under the action of the antibody-magnetic bead complex and the magnet, realizing the removal of non-target cells.

[0062] Embodiment Two

[0063] This embodiment also provides a cell filtration, sorting, enrichment and smear device.

[0064] Figure 9 Schematic diagram of the cell filtration, sorting, enrichment and smear device provided in the second embodiment of the present invention; Figure 10 Cross-sectional view showing the accommodation and resuspension mechanism provided in the second embodiment of the present invention. Refer to Figure 9 and Figure 10 , the difference between this embodiment and the first embodiment lies only in the structure of the accommodation and resuspension mechanism 100. In this embodiment, the sample accommodation component 110 and the resuspension component 120 are fixedly connected in a coaxial and integrated form.

[0065] Specifically, a first clamping structure 111 is provided at the end of the sample accommodation component 110, and the first clamping structure 111 can be fixed to the push-pull device of the filter. Only the resuspension component 120 is provided with a second threaded section 123 for connecting the filtering mechanism 200. And only the end of the resuspension component 120 is provided with a second stop cylinder 122 that can be inserted into the filtering mechanism 200 to block the through port 210.

[0066] The using process of the accommodation and resuspension mechanism 100 provided in Embodiment Two is as follows:

[0067] In this embodiment, the filter is a sealing syringe filter of a filtering device in the prior art, or can also be a device such as a centrifuge tube with a closed bottom. This embodiment does not make any limitations here. During use, the entire accommodating and resuspending mechanism 100 is directly connected to the filtering mechanism 200 by using the second threaded section 123 and inserted into the syringe of the filter. A filter membrane is provided in the filtering mechanism 200. At this time, the accommodating and resuspending mechanism 100 can accommodate a liquid biological sample. The first flap structure 111 is fixed to the pushing and pulling device of the filtering device. When the pushing and pulling device pulls the accommodating and resuspending mechanism 100, the cells in the liquid biological sample are enriched in the filtering mechanism 200 under the pulling action. Then, a resuspension liquid is injected into the accommodating and resuspending mechanism 100, and the whole of the accommodating and resuspending mechanism 100 and the filtering mechanism 200 is pushed axially into the mounting cylinder 310. During the above-mentioned pushing process, the cells enriched in the filtering mechanism 200 form a cell suspension with the resuspension liquid.

[0068] Obviously, the above-mentioned embodiments of the present invention are merely examples for clearly explaining the present invention, rather than limitations on the implementation manners of the present invention. For those of ordinary skill in the art, various obvious changes, re-adjustments and substitutions can be made without departing from the protection scope of the present invention. It is not necessary and impossible to enumerate all the implementation manners here. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. Cell filtration, sorting, enrichment and smear device, characterized in that Comprising: A containing and resuspending mechanism (100), which can be connected to a filter, including a sample containing component (110) and a resuspending component (120). The sample containing component (110) and the resuspending component (120) can be coaxially and integrally connected or independent of each other. The sample containing component (110) contains a liquid biological sample, and the resuspending component (120) contains a resuspension liquid; A filtering mechanism (200), which is detachably connected to the sample containing component (110) and can also be detachably connected to the resuspending component (120). A filter membrane is provided in the filtering mechanism (200). The liquid biological sample is filtered and enriched through the filter membrane and then resuspended by the resuspension liquid to obtain a cell suspension; A smearing mechanism (300), which is connected to a centrifugal smearing machine, can carry a glass slide and accommodate the filtering mechanism (200), and the cell suspension can flow from the filtering mechanism (200) to the glass slide.

2. The cell filtration, sorting, enrichment and smear device according to claim 1, wherein A first clamping flap structure (111) is provided at the end of the sample containing component (110), and the first clamping flap structure (111) can be fixed to the filter; and / or, A second clamping flap structure (121) is provided at the end of the resuspending component (120), and the second clamping flap structure (121) can be fixed to the filter.

3. The cell filtration, sorting, enrichment and smear device according to claim 1, wherein The filtering mechanism (200) is screwed to the sample containing component (110) or the resuspending component (120).

4. The cell filtration, sorting, enrichment and smear device according to claim 1, characterized in that, A first annular groove (112) is provided around the outer periphery of the sample containing component (110), and a first sealing ring is provided in the first annular groove (112). The first sealing ring can form a seal between the outer periphery of the sample containing component (110) and the inner wall of the filter.

5. The cell filtration, sorting, enrichment and smear device according to claim 1, wherein An opening (210) is formed on the side of the filtering mechanism (200), and the opening (210) is configured to allow the cell suspension passing through the filter membrane to flow out; A first stop cylinder (113) is provided at the end of the sample containing component (110), and the first stop cylinder (113) can be inserted into the filtering mechanism (200) to block the opening (210); and / or, A second stop cylinder (122) is provided at the end of the resuspending component (120), and the second stop cylinder (122) can be inserted into the filtering mechanism (200) to block the opening (210).

6. The cell filtration, sorting, enrichment and smear device according to claim 1, wherein, An annular boss (220) is provided around the inner wall of the filtering mechanism (200), and the annular boss (220) is used to fix the filter membrane.

7. The cell filtration, sorting, enrichment and smear device according to any one of claims 1-6, characterized in that, The smearing mechanism (300) includes an installation cylinder (310) and a slide component (320) which are connected in communication. The slide component (320) can position the glass slide and is connected to a centrifugal smearing machine. The installation cylinder (310) can accommodate the filtering mechanism (200), the outlet of the filtering mechanism (200) can be communicated with the slide component (320), and the cell suspension can flow to the glass slide.

8. The cell filtration, sorting, enrichment and smear device according to claim 7, characterized in that The bottom of the mounting cylinder (310) is provided with a mounting groove (312), and a magnet is arranged in the mounting groove (312). The magnet is configured to adsorb the magnetic material added to the liquid biological sample, and the magnetic material can bind to the non-target cells in the liquid biological sample.

9. The cell filtration, sorting and enrichment smear device according to claim 7, wherein The bottom of the mounting cylinder (310) is provided with a positioning bump (311), and the bottom of the filtering mechanism (200) is provided with a positioning groove (250). The positioning bump (311) can be inserted into the positioning groove (250) so that the outlet of the filtering mechanism (200) is directly opposite to the inlet of the slide assembly (320).

10. The cell filtration, sorting, enrichment and smear device according to claim 7, wherein The slide assembly (320) includes a carrier plate (321) and a clamping plate (322) which are movably connected. The mounting cylinder (310) is connected to the clamping plate (322), and a receiving groove is formed between the clamping plate (322) and the carrier plate (321), and the receiving groove accommodates the slide.

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