Sample preparation device and method for water environment detection

Abstract

The invention relates to the technical field of water environment detection, in particular to a sample preparation device and method for water environment detection.The sample preparation device comprises a test tube rack, a storage tank, a control table and a filling pipe; a jacking mechanism used for driving the test tube rack to vertically move and a horizontal moving mechanism used for driving the test tube rack to horizontally move are arranged in the control console, and according to the sample preparation device and method for water environment detection, a pretreated solution is added into the storage tank, so that the solution is quantitatively filled into the test tube. Moreover, after filling is completed, purified water can enter the filling pipe and the storage tank to automatically clean the filling pipe and the storage tank, a new solution can be conveniently added into the storage tank again by a laboratory technician, through the device, in the water pollution detection process, the sample taking speed of the laboratory technician can be increased, the rest time can be provided for the laboratory technician in the sample preparation process, and the experiment efficiency is improved. Therefore, a laboratory technician has strong attention to carry out subsequent instrument detection tests.

Description

Technical Field

[0001] The present invention relates to the technical field of water environment detection, and particularly relates to a sample preparation device and method for water environment detection. Background Art

[0002] For water environment detection, laboratory technicians need to take samples at multiple locations in the lake and send them to the laboratory for testing and processing, and judge the degree of water pollution based on the test results of samples taken from multiple locations. Before detection, pretreatment is required, and after pretreatment, the samples need to be prepared and detected. When preparing samples in the laboratory, the traditional method is to suck water into a pipette and transfer it into a test tube. When detecting in the laboratory, it is not just a few bottles of lake water to be detected in a day. Instead, the laboratory technicians detect the sewage samples sent by multiple groups of sampling technicians, and need to detect from morning to evening every day.

[0003] However, the repetitive sample preparation process consumes a lot of time. Also, a metering pump and a metering valve have been used to quantitatively pour the solution into the test tube, and the error can be controlled within the range of plus or minus 1 mg. However, inside the metering pump and the metering valve, the solution is poured through a sealed space and a valve core with a specific shape. After preparing one reagent, it is necessary to clean the inside for a long time to avoid the residue of the previous sewage inside. Moreover, the sewage in the storage tank is difficult to discharge, and it is difficult to clean the inside of the storage tank. This method is somewhat uneconomical.

[0004] Therefore, it is necessary to design a sample preparation device and method for water environment detection, which can not only quantitatively and quickly pour the solution into the test tube, but also clean the perfusion channel, valve body and storage tank, and the cleaning process is rapid and does not require manual operation. Summary of the Invention

[0005] Aiming at the above-mentioned technical deficiencies, the purpose of the present invention is to provide a sample preparation device and method for water environment detection, which can not only quantitatively and quickly pour the solution into the test tube, but also clean the perfusion channel, valve body and storage tank, and the cleaning process is rapid and does not require manual operation.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: the present invention provides a sample preparation device and method for water environment detection, including a test tube rack, a storage tank, a control console and a perfusion tube, the test tube rack is installed on the control console, and a lifting mechanism for driving the test tube rack to move vertically and a horizontal moving mechanism for driving the test tube rack to move horizontally are arranged in the control console, multiple rows of test tubes are placed on the test tube, and a branch tube for inserting into a single-row test tube is arranged at the bottom of the perfusion tube, and each branch tube is provided with a conical rubber plug for blocking the bottle mouth of the test tube, the storage tank is fixedly installed on the control console, and a bent pipe connected to the perfusion tube is arranged at the bottom of the storage tank, a transfer valve is arranged between the bent pipe and one end of the perfusion tube, a closing valve is arranged at the other end of the perfusion tube, and an air outlet pipe connected to the perfusion tube is arranged between the rightmost branch pipe and the closing valve, and the opening of the air outlet pipe is vertically upward.

[0007] Preferably, a three-way pipe is fixedly provided on the top of the air outlet pipe, the bottom of the three-way pipe is fixedly connected to the air outlet pipe, a solenoid valve is provided on the top of the three-way pipe, and the side of the three-way pipe is connected to the clean water pipe.

[0008] Preferably, the transfer valve includes a sleeve, a cover plate 1 and a horizontal sliding mechanism, the sleeve is sleeved on the outer edge of the end of the curved pipe, the curved pipe is inserted into the end of the sleeve and is provided with a conical head, the cover plate 1 is fixedly installed on the inner edge of the sleeve, a water permeable hole is opened on the cover plate 1, when the cover plate 1 is in contact with the conical head, the water permeable hole is located at the outer edge of the end of the conical head, the horizontal sliding mechanism is fixedly installed on the top of the console through a support frame, the horizontal sliding mechanism is used to push the cover plate 1 horizontally, the closing valve includes a cover plate 2 and a dislocation sliding mechanism, the dislocation sliding mechanism is fixedly installed on the support frame, and the dislocation sliding mechanism is used to push the cover plate 2 to cover the end of the perfusion pipe.

[0009] Preferably, the horizontal sliding mechanism is transmission-connected with the offset sliding mechanism, a compressible spring 1 is provided at the contact point between the horizontal sliding mechanism and the sleeve, and a compressible spring 2 is provided at the contact area between the offset sliding mechanism and the cover plate 2. When the spring 2 is compressed, the spring 1 is in a free state, and when the spring 1 is compressed, the spring 2 is in a free state.

[0010] Preferably, an electric push rod is arranged between the transfer valve and the closing valve, and the electric push rod is fixedly installed on the support frame. A hinge seat is fixedly arranged at the bottom of the output end of the electric push rod, and hinge rod 1 and hinge rod 2 are symmetrically arranged on the hinge seat. One end of hinge rod 1 is hinged to the hinge seat, and the other end of hinge rod 1 is hinged to the transfer valve, one end of hinge rod 2 is hinged to the hinge seat, and the other end of hinge rod 2 is hinged to the closing valve.

[0011] Preferably, the horizontal sliding mechanism includes a connecting plate, a sliding seat and two guide columns. The two guide columns can be installed on the top of the console for horizontal sliding. The middle parts of the sleeve and the guide columns are fixedly connected to the connecting plate. The sliding seat can be installed on the guide columns for horizontal sliding. A set of springs is arranged on the guide columns. The guide columns are used to apply an elastic force to the sliding seat away from the connecting plate. The hinged rod is hinged to the top of the sliding seat, and the tail of the guide column is provided with a limiting circular plate that contacts the sliding seat.

[0012] Preferably, the offset sliding mechanism includes a support frame, a sliding rod and a second sliding seat. The second sliding seat is horizontally slidably connected to the support frame through the sliding rod. The support frame is vertically fixedly installed on the top of the console. The perfusion pipe runs through the support frame. The second cover plate is fixedly installed on one end of the sliding column. The sliding column is slidably connected to the second sliding seat. The other end of the cover plate is fixedly provided with a second limiting circular plate. The second spring is sleeved on the sliding column. The second spring is used to apply an elastic force to the cover plate two away from the second sliding seat. One end of the second hinged rod is hinged to the second sliding seat, and the other end of the second hinged rod is hinged to the hinged seat.

[0013] Preferably, the test tube rack includes an outer shell, a plurality of displacement seats, and an elastic lifting mechanism. The outer shell is fixedly mounted on the lifting seat of the lifting mechanism. The plurality of displacement seats are mounted on the outer shell in a horizontal arrangement. The elastic lifting mechanism is fixedly mounted in the outer shell and is used to lift the displacement seats upward. Each displacement seat includes a base plate, a plurality of extension columns and a contact rod. The base plate is located in the outer shell, and the bottom of the base plate contacts the top of the elastic lifting mechanism. The extension column is vertically fixedly mounted on the top of the base plate. The extension column is slidably connected to the outer shell. The contact rod is fixedly mounted on the top of the extension column. A snap ring is provided on the base plate. The test tube passes through the contact rod, the snap ring and the base plate and contacts the elastic lifting mechanism. Both the contact rod and the base plate are provided with through holes for the test tube to pass through. A lower push rod in contact with the contact rod is fixedly provided on the hinged seat.

[0014] Preferably, the elastic lifting mechanism includes a lifting plate, a lifting spring and a cross beam. The cross beam is fixedly mounted on the outer shell. The lifting plate can be vertically slidably mounted on the cross beam through vertical guide columns. The lifting spring is used to provide an elastic force to move the lifting plate away from the cross beam. The top of the lifting plate contacts the bottom of the test tube.

[0015] A method for preparing a sample for water environment testing: Step 1: Insert multiple rows of test tubes into the contact rod; Step 2: The horizontal moving mechanism and the lifting mechanism drive the shell to move so that the test tube is engaged with the conical rubber plug; Step 3: The electric push rod is pulled upward to reset, the transfer valve is opened, the closing valve is closed, and the solution in the storage tank flows into multiple test tubes along the perfusion tube; Step 4: After the infusion reaches the preset time, the electric push rod is pushed downward, the transfer valve is closed, the closing valve is opened, and the excess solution in the infusion tube is discharged; Step Five: The electric push rod pushes the lower push rod downward, and the test tube moves downward. If it cannot be separated from the conical rubber stopper, it is manually pulled out downward. The solution inside the branch tube and the outer edge of the conical rubber stopper drips into the test tube statically; Step Six: Switch to another row of test tubes for perfusion. After all the perfusion is completed, connect one row of test tubes to the conical rubber stopper. The solenoid valve connected to the three-way pipe is closed, the transfer valve is opened, the closing valve is closed, and then pure water is injected into the air outlet pipe to wash the inside of the storage tank and the perfusion pipe; Step Seven: Stop injecting pure water, and alternately open and close the transfer valve and the closing valve to drain the pure water in the perfusion pipe; Step Eight: Repeat the process of Steps One to Seven until all the test tubes are perfused. The beneficial effects of the present invention are as follows: For the sample preparation device and method for water environment detection, the pretreated solution is added to the storage tank. The transfer valve and the closing valve are used to control the solution in the storage tank to flow into the perfusion pipe, and the conical rubber stopper is clamped with the tube orifice of the test tube, so that the solution can be automatically filled into the test tube, and the excess solution can be quickly discharged after the closing valve is opened during the filling process, realizing quantitative filling of the solution into the test tube.

[0016] And after the filling is completed, the solution in the storage tank can be discharged by alternately opening and closing the transfer valve and the closing valve. By injecting pure water into the three-way pipe, the pure water can enter the perfusion pipe and the storage tank to automatically clean them. After the automatic cleaning is completed, it is convenient for the experimenter to add a new solution into the storage tank again.

[0017] Moreover, the structure of opening and closing the transfer valve is mainly carried out by using Cover Plate One, which is easy to clean and avoids the residual experimental solution of the previous time in the valve body. And the whole cleaning process is rapid and does not require manual operation.

[0018] By pushing downward with the electric push rod, the transfer valve and the closing valve are alternately opened and closed. The opening and closing process is rapid, and the risk of all the solution flowing out due to the simultaneous opening of the transfer valve and the closing valve, resulting in the invalidation of all experimental data, is avoided.

[0019] And before the test tube moves after the filling is completed, the electric push rod can push the lower push rod downward to check whether all the test tubes can descend, avoiding the fragmentation of the test tubes caused by clamping when the subsequent lifting mechanism and horizontal moving mechanism drive the test tubes to move. Description of the Drawings

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0021] Figure 1 Schematic three-dimensional structure of the present invention Figure 1 。

[0022] Figure 2 Schematic three-dimensional structure of the present invention Figure 2 。

[0023] Figure 3 Front view of the present invention.

[0024] Figure 4 Schematic three-dimensional structure diagram of the horizontal pushing mechanism.

[0025] Figure 5 Partial cross-sectional view of the present invention.

[0026] Figure 6 Schematic three-dimensional structure diagram of the offset pushing mechanism.

[0027] Figure 7 Cross-sectional view of the closing gate.

[0028] Figure 8 Cross-sectional view of the test tube rack.

[0029] Figure 9 Exploded schematic three-dimensional structure diagram of the test tube rack.

[0030] Figure 10 Cross-sectional view of the test tube installation state.

[0031] Description of reference numerals: 1. Test tube rack; 1a. Outer shell; 1b. Cross beam; 1c. Displacement seat; 1c1. Bottom plate; 1c2. Extension column; 1c3. Contact rod; 1c4. Snap ring; 1d. Lifting plate; 1e. Lifting spring; 2. Test tube; 3. Storage tank; 3a. Elbow pipe; 3a1. Tapered head; 4. Console; 4a. Lifting mechanism; 4b. Horizontal movement mechanism; 5. Transfer valve; 5a. Sleeve; 5b. Cover plate I; 5b1. Water permeable hole; 5c. Horizontal pushing mechanism; 5c1. Spring I; 5c2. Connecting plate; 5c3. Guide post; 5c4. Slide seat I; 6. Perfusion pipe; 6a. Branch pipe; 6b. Air outlet pipe; 6c. Tapered rubber plug; 6d. Three-way pipe; 7. Closing valve; 7a. Cover plate II; 7b. Dislocation pushing mechanism; 7b1. Spring II; 7b2. Support frame; 7b3. Slide bar; 7b4. Slide seat II; 7b5. Slide post; 8. Electric push rod; 8a. Hinge seat; 8b. Hinge rod I; 8c. Hinge rod II; 9. Lower push rod. Detailed implementation manners

[0032] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0033] Embodiment: The present invention provides a sample preparation device and method for water environment detection, as Figures 1 - 10As shown, it includes a test tube rack 1, a storage tank 3, a control console 4 and a perfusion tube 6. The test tube rack 1 is installed on the control console 4. A lifting mechanism 4a for driving the test tube rack 1 to move vertically and a horizontal moving mechanism 4b for driving the test tube rack 1 to move horizontally are arranged in the control console 4. Multiple rows of test tubes 2 are placed on the test tube 2. A branch tube 6a for inserting into the single-row test tube 2 is arranged at the bottom of the perfusion tube 6. Each branch tube 6a is provided with a conical rubber plug 6c for blocking the bottle mouth of the test tube 2. The storage tank 3 is fixedly installed on the control console 4. A bent pipe 3a connected to the perfusion tube 6 is arranged at the bottom of the storage tank 3. A transfer valve 5 is arranged between the bent pipe 3a and one end of the perfusion tube 6. A closing valve 7 is arranged at the other end of the perfusion tube 6. An outlet pipe 6b connected to the perfusion tube 6 is arranged between the rightmost branch pipe 6a and the closing valve 7, and the opening of the outlet pipe 6b is vertically upward. When it is necessary to prepare a sample, first pour the pretreated aqueous solution into the storage tank 3, and the transfer valve 5 is in a closed state. Then push the test tube rack 1 upward through the lifting mechanism 4a, so that the end of the branch tube 6a is inserted into a whole row of test tubes 2, and the conical rubber plug 6c blocks and seals the tube mouth of the test tube 2. Then the transfer valve 5 is opened, and the solution in the storage tank 3 will enter the test tube 2 along the multiple branch tubes 6a, so that the first row of test tubes 2 is filled. Among them, the amount of solution poured each time is controlled according to the opening and closing time of the transfer valve 5. After each filling is completed, there will always be some extra solution in the filling tube 6, and then the extra solution can be automatically discharged by opening the closing valve 7.

[0034] When the first row of solution is filled, the test tube 2 is driven downward by the lifting mechanism 4 a , and the excess solution in the branch tube 6 a will drip into the test tube 2 .

[0035] Then, a row of test tubes 2 is horizontally moved by the horizontal moving mechanism 4b, and the above process is repeated to fill a new row of test tubes 2.

[0036] This filling method can fill the test tube 2 with the solution faster, and can increase the speed in the laboratory, and can detect water pollution in multiple locations faster. In addition, this filling method has no error in the amount of solution in the test tube 2, and will not be affected by equipment failure and cause the amount of solution to be wrong.

[0037] A three-way pipe 6d is fixedly arranged at the top of the air outlet pipe 6b. The bottom of the three-way pipe 6d is fixedly connected to the air outlet pipe 6b. An electromagnetic valve is arranged at the top of the three-way pipe 6d. The side of the three-way pipe 6d is connected to a water purification pipe, which is not shown in the figure. When it is necessary to clean the perfusion pipe 6, the transfer valve 5 and its storage tank 3, first, block and seal the gap between one row of test tubes 2 and the conical rubber plug 6c. Then, supply water through the water purification machine connected by the water purification pipe. At this time, since the closing valve 7 is in the closed state and the transfer valve 5 is in the open state, the water source will flow along the perfusion pipe 6 through the transfer valve 5 and enter the storage tank 3 for perfusion. After perfusion, by alternately opening and closing the transfer valve 5 and the closing valve 7 multiple times, the alternate opening is also for cleaning the valve, which will cause the water inside the storage tank 3 and the perfusion pipe 6 to flow out along the end of the perfusion pipe 6, that is, automatic cleaning is achieved. It should be noted that one row of the multiple rows of test tubes 2 is dedicated to cleaning and does not pour the solution.

[0038] During the above perfusion process, the electromagnetic valve connected to the three-way pipe 6d is in the closed state, and the opening of the electromagnetic valve is mainly for discharging air when the solution flows in the perfusion pipe 6.

[0039] The transfer valve 5 includes a sleeve 5a, a first cover plate 5b, and a horizontal pushing mechanism 5c. The sleeve 5a is sleeved on the outer edge of the end of the elbow pipe 3a. A conical head 3a1 is arranged at the end of the elbow pipe 3a inserted into the sleeve 5a. The first cover plate 5b is fixedly installed on the inner edge of the sleeve 5a. A water permeable hole 5b1 is opened on the first cover plate 5b. When the first cover plate 5b fits with the conical head 3a1, the water permeable hole 5b1 is located at the outer edge of the end of the conical head 3a1. The horizontal pushing mechanism 5c is fixedly installed on the top of the console 4 through a support frame. The horizontal pushing mechanism 5c is used to horizontally push the first cover plate 5b. By controlling the horizontal pushing mechanism 5c to work, the horizontal pushing mechanism 5c will push the sleeve 5a to move horizontally, so that the sleeve 5a drives the first cover plate 5b to move horizontally, and the first cover plate 5b will cover the end of the conical head 3a1, sealing the end of the elbow pipe 3a. When the first cover plate 5b is opened, the solution will pass through the water permeable hole 5b1 and enter the perfusion pipe 6 to pour the solution into the test tube 2. The structure of the transfer valve 5 is simple. When the subsequent purified water enters the pipeline for cleaning, only the first cover plate 5b needs to be cleaned. The cleaning process of the valve body is simple and rapid, and the purified water entering the pipeline can enter the storage tank 3 along the elbow pipe 3a to clean the inside of the storage tank 3, and the cleaned water can flow back to the perfusion pipe 6 along the elbow pipe 3a. The closing valve 7 includes a second cover plate 7a and a dislocation pushing mechanism 7b. The dislocation pushing mechanism 7b is fixedly installed on the support frame. The dislocation pushing mechanism 7b is used to push the second cover plate 7a to cover the end of the perfusion pipe 6. The second cover plate 7a is pushed by the dislocation pushing mechanism 7b to cover the end of the perfusion pipe 6, that is, the sealing of the tail of the perfusion pipe 6 is achieved.

[0040] The horizontal push mechanism 5c is connected to the offset push mechanism 7b in transmission. The contact area between the horizontal push mechanism 5c and the sleeve 5a is provided with a spring 1 5c1 that can be compressed. The contact area between the offset push mechanism 7b and the cover plate 2 7a is provided with a spring 2 7b1 that can be compressed. When the spring 2 7b1 is compressed, the spring 1 5c1 is in a free state. When the spring 1 5c1 is compressed, the spring 2 7b1 is in a free state. The horizontal push mechanism 5c and the offset push mechanism 7b move simultaneously, which can ensure that when the conical head 3a1 is closed, the perfusion tube 6 can be opened. At the same time, it can ensure that when the perfusion tube 6 is closed, the conical head 3a1 can be opened, so as to avoid the transfer valve 5 and the closing valve 7 being opened at the same time, which will cause all the solutions in the storage tank 3 to leak out. If all the solutions leak out, multiple test results will be invalid, and all samples need to be taken again. The whole movement process is as follows: when the horizontal push mechanism 5c pushes the cover plate 1 5b to cover the conical head 3a1, the offset push mechanism 7b gradually moves away from the perfusion tube 6, and the resistance of the spring 2 7b1 enables the cover plate 2 7a to be pressed against the perfusion tube 6. When the cover plate 1 5b covers the conical head 3a1, the cover plate 2 7a still keeps in contact with the perfusion tube 6. As the movement continues, the cover plate 2 7a separates from the perfusion tube 6, and the spring 1 5c1 is compressed, that is, the residual solution in the perfusion tube 6 is discharged outward.

[0041] By providing the spring 1 5c1 and the spring 2 7b1, the cover plate 1 5b and the cover plate 2 7a can be elastically pressed together, so that they are squeezed more tightly.

[0042] An electric push rod 8 is arranged between the transfer valve 5 and the closing valve 7. The electric push rod 8 is fixedly mounted on the support frame. A hinge seat 8a is fixedly arranged at the bottom of the output end of the electric push rod 8. A hinge rod 1 8b and a hinge rod 2 8c are symmetrically arranged on the hinge seat 8a. One end of the hinge rod 1 8b is hinged to the hinge seat 8a, and the other end of the hinge rod 1 8b is hinged to the transfer valve 5. One end of the hinge rod 2 8c is hinged to the hinge seat 8a, and the other end of the hinge rod 2 8c is hinged to the closing valve 7. By pushing the hinge seat 8a downward, the electric push rod 8 can simultaneously push the hinge rod 1 8b and the hinge rod 2 8c, that is, the transfer valve 5 and the closing valve 7 are driven to move at the same time. In this process, the amplitude of the movement can be adjusted according to the angle between the hinge rod 1 8b and the hinge rod 2 8c, so that the movement amplitude of the cover plate 2 7a and the sleeve 5a can be just right.

[0043] The horizontal push mechanism 5c includes a connecting plate 5c2, a sliding seat 5c4 and two guide posts 5c3. The two guide posts 5c3 can be installed on the top of the console 4 in a horizontal sliding manner. A support seat is provided at the bottom of the storage tank 3. The guide posts 5c3 are inserted into the support seat. The support seat is provided with guide holes for the guide posts 5c3 to slide. The middle parts of the sleeve 5a and the guide posts 5c3 are fixedly connected to the connecting plate 5c2. The sliding seat 5c4 can be installed on the guide posts 5c3 in a horizontal sliding manner. The spring 5c1 is sleeved on the guide posts 5c3. The guide posts 5c3 are used to apply pressure to the sliding seat 5c4. Away from the elastic force of the connecting plate 5c2, the hinged rod 8b is hinged at the top of the slide 5c4, and the tail of the guide column 5c3 is provided with a limiting circular plate 1 that contacts the slide 5c4. When the hinged rod 8b is pushed downward, the hinged rod 8b will move outward toward the slide 5c4, so that the cover plate 5b is covered on the conical head 3a1, and the hinged rod 8b continues to move downward, which will compress the spring 5c1 to separate the cover plate 7a from the perfusion tube 6, and at the same time, leave room for the push rod 9 to push downward.

[0044] The dislocation pushing mechanism 7b includes a support frame 7b2, a slide bar 7b3 and a second slide seat 7b4. The second slide seat 7b4 is horizontally slidably connected with the support frame 7b2 through the slide bar 7b3. The support frame 7b2 is vertically fixedly installed on the top of the console 4. The perfusion pipe 6 runs through the support frame 7b2. The second cover plate 7a is fixedly installed on one end of the slide column 7b5. The slide column 7b5 is slidably connected with the second slide seat 7b4. The other end of the second cover plate 7a is fixedly provided with a second limited circular plate. The second spring 7b1 is sleeved on the slide column 7b5. The second spring 7b1 is used to apply an elastic force to the second cover plate 7a away from the second slide seat 7b4. One end of the second hinge rod 8c is hinged with the second slide seat 7b4, and the other end of the second hinge rod 8c is hinged with the hinge seat 8a. When the second hinge rod 8c is pressed downward, the second hinge rod 8c will push the second slide seat 7b4 away from the perfusion pipe 6, so that the second spring 7b1 component recovers its shape. After the shape of the second spring 7b1 is restored, the second hinge rod 8c continues to press downward, and the second hinge rod 8c will push the second slide seat 7b4 to move outward, so that the second cover plate 7a is separated from the perfusion tube 6, and the solution stored in the perfusion tube 6 can be discharged.

[0045] The test tube rack 1 includes a shell 1a, a plurality of displacement seats 1c, and an elastic lifting mechanism. The shell 1a is fixedly mounted on the lifting seat of the lifting mechanism 4a. The plurality of displacement seats 1c are mounted on the shell 1a in a horizontal arrangement. The elastic lifting mechanism is fixedly mounted in the shell 1a and is used to lift the displacement seats 1c upward. Each displacement seat 1c includes a bottom plate 1c1, a plurality of extension columns 1c2, and a contact rod 1c3. The bottom plate 1c1 is located in the shell 1a, and the bottom of the bottom plate 1c1 contacts the top of the elastic lifting mechanism. , the extension column 1c2 is vertically fixedly installed on the top of the bottom plate 1c1, the extension column 1c2 is slidably connected with the housing 1a, the contact rod 1c3 is fixedly installed on the top of the extension column 1c2, the bottom plate 1c1 is provided with a snap ring 1c4, the test tube 2 passes through the contact rod 1c3, the snap ring 1c4 and the bottom plate 1c1 and contacts with the elastic lifting mechanism, the contact rod 1c3 and the bottom plate 1c1 are both provided with through holes for the test tube 2 to pass through, and the hinge seat 8a is fixedly provided with a lower push rod 9 in contact with the contact rod 1c3. Because after the lifting mechanism 4a is pushed upward, the end of the test tube 2 will be squeezed with the conical rubber plug 6c, resulting in a tight clamping between the diameter of the test tube 2 and the conical rubber plug 6c, and when the lifting mechanism 4a is lowered, there is a probability that the test tube 2 cannot move downward together, if the horizontal moving mechanism 4b directly moves horizontally at this time, the stuck test tube 2 will be directly broken. Although a circle of rubber pad is provided inside the clamp ring 1c4 to fit tightly with the test tube 2, it is still impossible to separate all the test tubes 2 during long-term use.

[0046] Therefore, after the electric push rod 8 is pushed downward to discharge the solution in the perfusion tube 6, the lower push rod 9 contacts the top of the contact rod 1c3, and then the lower push rod 9 continues to push downward, which will cause the displacement seat 1c to drive the entire row of test tubes 2 to descend in advance. The experimenter can observe whether the test tube 2 is separated from the conical rubber plug 6c at this time. If there is no separation, the experimenter can pull down the corresponding test tube 2 that cannot be moved downward to prevent the test tube 2 from breaking when moving horizontally. This process does not consume extra time, because the solution remaining in the conical rubber plug 6c and the outer edge of the conical rubber plug 6c needs a time process to drip into the test tube 2.

[0047] When the displacement seat 1c descends, the movement of the housing 1a is guided by the extension column 1c2, and the displacement seat 1c presses the elastic lifting mechanism downward, so that the displacement seat 1c can descend.

[0048] The elastic lifting mechanism includes a lifting plate 1d, a lifting spring 1e, and a cross beam 1b. The cross beam 1b is fixedly installed on the outer shell 1a. The lifting plate 1d is installed on the cross beam 1b through a vertical guide post and can slide vertically. The lifting spring 1e is used to provide an elastic force for the lifting plate 1d to move away from the cross beam 1b. The top of the lifting plate 1d contacts the bottom of the test tube 2. When the displacement seat 1c is squeezed downward, the lifting plate 1d will be pushed downward, causing the lifting spring 1e to be compressed. When the test tube 2 is inserted into the smart card ring 1c4, if the insertion force is too large, it will push the lifting plate 1d downward, but afterwards, it can be pushed by the elastic force to fit with the bottom plate 1c1.

[0049] A method for a sample preparation device for water environment detection: Step 1: Insert multiple rows of test tubes 2 into the contact rod 1c3; Step 2: The horizontal movement mechanism 4b and the lifting mechanism 4a drive the outer shell 1a to move, so that the test tube 2 is clamped with the conical rubber plug 6c; Step 3: The electric push rod 8 pulls upward to reset, the transfer valve 5 opens, the closing valve 7 closes, presenting Figure 3 the state shown, and the solution in the storage tank 3 flows into multiple test tubes 2 along the perfusion tube 6; Step 4: After perfusion for a preset time, the electric push rod 8 pushes downward, the transfer valve 5 closes, the closing valve 7 opens, and the excess solution in the perfusion tube 6 is discharged; Step 5: The electric push rod 8 pushes the lower push rod 9 downward, the test tube 2 moves downward. If it cannot be separated from the conical rubber plug 6c, it is manually pulled out downward, and the solution in the branch tube 6a and the outer edge of the conical rubber plug 6c stands still and drips into the test tube 2; Step 6: Switch to another row of test tubes 2 for perfusion. After all the perfusion is completed, connect one row of test tubes 2 with the conical rubber plug 6c, close the solenoid valve connected to the three-way pipe 6d, open the transfer valve 5, close the closing valve 7, and then inject pure water into the air outlet pipe 6b to flush the inside of the storage tank 3 and the perfusion tube 6; Step 7: Stop injecting pure water, and the transfer valve 5 and the closing valve 7 are alternately opened and closed to discharge the pure water in the perfusion tube 6; Step 8: Repeat the process of Step 1 to Step 7 until all the test tubes 2 are perfused.

[0050] For the sample preparation device and method for water environment detection, by adding the pre-treated solution to the storage tank 3, controlling the solution in the storage tank 3 to flow into the perfusion tube 6 through the transfer valve 5 and the closing valve 7, and clamping the conical rubber plug 6c with the mouth of the test tube 2, the solution can be automatically filled into the test tube 2, and the excess solution during the filling process can be quickly discharged after the closing valve 7 is opened, realizing quantitative filling of the solution into the test tube 2.

[0051] And after the filling is completed, the solution in the storage tank 3 can be discharged by alternately opening and closing the transfer valve 5 and closing the valve 7, and by injecting pure water into the three-way pipe 6d, the pure water can enter the perfusion pipe 6 and the storage tank 3 to automatically clean them. After the automatic cleaning is completed, it is convenient for the experimenter to add a new solution into the storage tank 3 again.

[0052] Moreover, the structure of opening and closing the transfer valve 5 is mainly carried out by using the first cover plate 5b, which is convenient for cleaning and avoids the residual experimental solution of the previous time in the valve body. And the whole cleaning process is rapid and does not require manual operation.

[0053] By the downward push of the electric push rod 8, the transfer valve 5 and the closing valve 7 are alternately opened and closed. The opening and closing process is rapid, and the risk of all the solution flowing out due to the simultaneous opening of the transfer valve 5 and the closing valve 7, resulting in the invalidation of all experimental data, is avoided.

[0054] And before the test tube 2 moves after the filling is completed, the electric push rod 8 can push the lower push rod 9 downward to check whether all the test tubes 2 can descend, so as to avoid the fragmentation of the test tubes 2 caused by jamming when the subsequent lifting mechanism 4a and the horizontal moving mechanism 4b drive the test tubes 2 to move.

[0055] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these changes and modifications.

Claims

1. A sample preparation device for water environment detection, characterized in that, The invention comprises a test tube rack (1), a storage tank (3), a control console (4) and a perfusion tube (6), wherein the test tube rack (1) is mounted on the control console (4), wherein a lifting mechanism (4a) for driving the test tube rack (1) to move vertically and a horizontal moving mechanism (4b) for driving the test tube rack (1) to move horizontally are arranged in the control console (4), wherein multiple rows of test tubes (2) are placed on the test tube rack (2), and a branch tube (6a) for inserting into a single row of test tubes (2) is arranged at the bottom of the perfusion tube (6), and each branch tube (6a) is provided with a The mouth of the test tube (2) is blocked by a conical rubber stopper (6c); the storage tank (3) is fixedly mounted on the console (4); a bent pipe (3a) connected to the perfusion pipe (6) is provided at the bottom of the storage tank (3); a transfer valve (5) is provided between the bent pipe (3a) and one end of the perfusion pipe (6); a closing valve (7) is provided at the other end of the perfusion pipe (6); an air outlet pipe (6b) connected to the perfusion pipe (6) is provided between the rightmost branch pipe (6a) and the closing valve (7); the opening of the air outlet pipe (6b) is vertically upward.

2. The sample preparation device for water environment detection according to claim 1, characterized in that, A three-way pipe (6d) is fixedly arranged on the top of the air outlet pipe (6b), the bottom of the three-way pipe (6d) is fixedly connected to the air outlet pipe (6b), a solenoid valve is arranged on the top of the three-way pipe (6d), and the side of the three-way pipe (6d) is connected to the clean water pipe.

3. The sample preparation device for water environment detection according to claim 1, characterized in that, The transfer valve (5) comprises a sleeve (5a), a cover plate (5b) and a horizontal push mechanism (5c); the sleeve (5a) is sleeved on the outer edge of the end of the curved pipe (3a); the curved pipe (3a) is inserted into the end of the sleeve (5a) and is provided with a conical head (3a1); the cover plate (5b) is fixedly mounted on the inner edge of the sleeve (5a); a water permeable hole (5b1) is provided on the cover plate (5b); when the cover plate (5b) is fitted with the conical head (3a1), the water permeable hole (5b1) is opened. 1) is located at the outer edge of the end of the conical head (3a1), the horizontal push mechanism (5c) is fixedly installed on the top of the control console (4) through a support frame, the horizontal push mechanism (5c) is used to horizontally push the cover plate 1 (5b), the closing valve (7) includes a cover plate 2 (7a) and a dislocation push mechanism (7b), the dislocation push mechanism (7b) is fixedly installed on the support frame, and the dislocation push mechanism (7b) is used to push the cover plate 2 (7a) to cover the end of the perfusion pipe (6).

4. The sample preparation device for water environment detection according to claim 3, characterized in that, The horizontal push mechanism (5c) is transmission-connected to the offset push mechanism (7b); a spring 1 (5c1) capable of being compressed is provided at the contact point between the horizontal push mechanism (5c) and the sleeve (5a); a spring 2 (7b1) capable of being compressed is provided at the contact area between the offset push mechanism (7b) and the cover plate 2 (7a); when the spring 2 (7b1) is compressed, the spring 1 (5c1) is in a free state; and when the spring 1 (5c1) is compressed, the spring 2 (7b1) is in a free state.

5. The sample preparation device for water environment detection according to claim 4, wherein, An electric push rod (8) is arranged between the transfer valve (5) and the closing valve (7). The electric push rod (8) is fixedly installed on the support frame. At the bottom of the output end of the electric push rod (8), a hinge seat (8a) is fixedly arranged. On the hinge seat (8a), a first hinge rod (8b) and a second hinge rod (8c) are symmetrically arranged. One end of the first hinge rod (8b) is hinged to the hinge seat (8a), and the other end of the first hinge rod (8b) is hinged to the transfer valve (5). One end of the second hinge rod (8c) is hinged to the hinge seat (8a), and the other end of the second hinge rod (8c) is hinged to the closing valve (7).

6. The sample preparation device for water environment detection according to claim 5, characterized in that, The horizontal pushing mechanism (5c) includes a connecting plate (5c2), a first sliding seat (5c4), and two guide columns (5c3). The two guide columns (5c3) are installed on the top of the control console (4) so as to be horizontally slidable. The middle parts of the sleeve (5a) and the guide columns (5c3) are fixedly connected to the connecting plate (5c2). The first sliding seat (5c4) is installed on the guide columns (5c3) so as to be horizontally slidable. A first spring (5c1) is sleeved on the guide columns (5c3). The guide columns (5c3) are used to apply an elastic force to the first sliding seat (5c4) to move it away from the connecting plate (5c2). One end of the first hinge rod (8b) is hinged to the top of the first sliding seat (5c4). At the tail of the guide columns (5c3), a first limiting circular plate that abuts against the first sliding seat (5c4) is provided.

7. The sample preparation device for water environment detection according to claim 5, characterized in that, The dislocation pushing mechanism (7b) includes a support frame (7b2), a sliding rod (7b3), and a second sliding seat (7b4). The second sliding seat (7b4) is horizontally slidably connected to the support frame (7b2) through the sliding rod (7b3). The support frame (7b2) is vertically and fixedly installed on the top of the control console (4). The perfusion pipe (6) passes through the support frame (7b2). A second cover plate (7a) is fixedly installed at one end of the sliding column (7b5). The sliding column (7b5) is slidably connected to the second sliding seat (7b4). At the other end of the second cover plate (7a), a second limiting circular plate is fixedly arranged. A second spring (7b1) is sleeved on the sliding column (7b5). The second spring (7b1) is used to apply an elastic force to the second cover plate (7a) to move it away from the second sliding seat (7b4). One end of the second hinge rod (8c) is hinged to the second sliding seat (7b4), and the other end of the second hinge rod (8c) is hinged to the hinge seat (8a).

8. The sample preparation device for water environment detection according to claim 5, characterized in that The test tube rack (1) includes a housing (1a), a plurality of displacement seats (1c), and an elastic lifting mechanism. The housing (1a) is fixedly installed on the lifting seat of the lifting mechanism (4a). The plurality of displacement seats (1c) are horizontally arranged and installed on the housing (1a). The elastic lifting mechanism is fixedly installed inside the housing (1a) and is used to lift the displacement seat (1c) upward. Each displacement seat (1c) includes a bottom plate (1c1), a plurality of extension columns (1c2), and a contact rod (1c3). The bottom plate (1c1) is located inside the housing (1a). The bottom of the bottom plate (1c1) contacts the top of the elastic lifting mechanism. The extension columns (1c2) are vertically and fixedly installed on the top of the bottom plate (1c1). The extension columns (1c2) are slidably connected to the housing (1a). The contact rod (1c3) is fixedly installed on the top of the extension column (1c2). A snap ring (1c4) is provided on the bottom plate (1c1). The test tube (2) passes through the contact rod (1c3), the snap ring (1c4), and the bottom plate (1c1) and abuts against the elastic lifting mechanism. Through holes for the test tube (2) to pass through are provided on both the contact rod (1c3) and the bottom plate (1c1). A lower push rod (9) that contacts the contact rod (1c3) is fixedly provided on the hinge seat (8a).

9. The sample preparation device for water environment detection according to claim 9, characterized in that, The elastic lifting mechanism includes a lifting plate (1d), a lifting spring (1e), and a cross beam (1b). The cross beam (1b) is fixedly installed on the housing (1a). The lifting plate (1d) is installed on the cross beam (1b) through vertical guide columns and can slide vertically. The lifting spring (1e) is used to provide an elastic force for the lifting plate (1d) away from the cross beam (1b). The top of the lifting plate (1d) contacts the bottom of the test tube (2).

10. The method of a sample preparation device for water environment detection according to claim 8, wherein: Step 1: Insert multiple rows of test tubes (2) into the contact rod (1c3); Step 2: The horizontal movement mechanism (4b) and the lifting mechanism (4a) drive the housing (1a) to move, so that the test tube (2) is clamped with the conical rubber plug (6c); Step 3: The electric push rod (8) pulls upward to reset, the transfer valve (5) is opened, the closing valve (7) is closed, and the solution in the storage tank (3) flows into the multiple test tubes (2) along the perfusion tube (6); Step 4: After perfusion for a preset time, the electric push rod (8) pushes downward, the transfer valve (5) is closed, the closing valve (7) is opened, and the excess solution in the perfusion tube (6) is discharged; Step 5: The electric push rod (8) pushes the lower push rod (9) downward, the test tube (2) moves downward. If it cannot be separated from the conical rubber plug (6c), it is manually pulled out downward, and the solution on the outer edge of the conical rubber plug (6c) in the branch tube (6a) stands and drips into the test tube (2); Step 6: Switch to another row of test tubes (2) for perfusion. After all perfusion is completed, clamp one row of test tubes (2) with the conical rubber plug (6c), close the solenoid valve connected to the three-way pipe (6d), open the transfer valve (5), close the closing valve (7), and then inject pure water into the air outlet pipe (6b) to flush the inside of the storage tank (3) and the perfusion tube (6); Step 7: Stop injecting pure water. The transfer valve (5) and the closing valve (7) are alternately opened and closed to drain the pure water in the perfusion tube (6). Step 8: Repeat the process of Step 1 to Step 7 until all the test tubes (2) are completely perfused.

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