Fluorescence detection device capable of preventing test tube from being damaged and used for production of polygonatum sibiricum concentrated enzyme

Abstract

The invention relates to the technical field of biology, in particular to a fluorescence detection device for production of polygonatum sibiricum concentrated enzymes and capable of preventing test tubes from being damaged, which comprises a fluorescence detection mechanism, when an annular plate moves upwards, air pressure in a cavity formed by a middle protective cover and an outer protective cover is reduced, and when a valve core moves leftwards, a conical part of the valve core pushes an ejector rod to move; the other end of the ejector rod pushes a triangular block to move transversely, so that a circular ring I drives a circular ring II to move rightwards to extrude fluorescent powder, and meanwhile, a spray head generates suction force to a connecting hole, so that liquid in a test tube flows out of a crack more easily, the fluorescent powder is scattered to the position of the connecting hole after being extruded, and the liquid seeps out of the connecting hole to generate fluorescent reaction; the fluorescent agent is triggered to be injected into the test tube through the air pressure change, whether the test tube is damaged or not is rapidly judged, fluorescent powder is sprayed to the crack of the outer wall of the test tube through the valve assembly, damage positioning is achieved in combination with seepage fluorescence reaction, and the detection precision is remarkably improved.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and specifically to a fluorescence detection device for the production of polygonatum sibiricum concentrated enzyme to prevent test tube breakage. Background Art

[0002] The background art of the existing fluorescence detection device for preventing test tube breakage mainly realizes double protection through physical buffering (such as silica gel sleeves / elastic brackets) combined with fluorescence labeling monitoring technology. The fluorescence material coated or embedded is used to cooperate with the optical sensor to detect breakage and leakage in real time, and the pressure sensing or image analysis algorithm is integrated to realize active warning, promoting the upgrade of laboratory safety from passive protection to intelligent dynamic monitoring; An existing food fluorescence detection device with the publication number of CN109520986A relates to the field of food detection technology. It includes a fluorescence detection carrier, and a detection groove is opened on the upper surface of the fluorescence detection carrier. A fixing plate is arranged above the detection groove, a connecting plate is fixedly connected to the upper surface of the fixing plate, a clamping groove is opened on the front surface of the fixing plate, and first grooves are opened on the left and right sides of the connecting plate. In this food fluorescence detection device, by setting the fixing plate, the clamping groove, the connecting plate, the connecting rod, the first spring and the clamping block, people can clamp the test tube in the clamping groove to stably fix the test tube on the fixing plate. Then, by pressing the connecting plate downward, the test tube on the fixing plate can be inserted into the detection groove. At the same time, the two clamping blocks can be clamped into the two limiting grooves, which avoids the test tube being broken due to collision when people directly insert the test tube into the detection groove, thus bringing convenience for people to insert the test tube into the detection groove; Traditional fluorescence detection only judges breakage by the leakage of the fluorescent agent inside the test tube, cannot locate the breakage point, and is easily interfered by the liquid viscosity or bubbles. Summary of the Invention

[0003] The purpose of the present invention is to provide a fluorescence detection device for the production of polygonatum sibiricum concentrated enzyme to prevent test tube breakage, so as to solve the problems raised in the above background art.

[0004] To achieve the above-mentioned invention purpose, the present invention adopts the following technical solutions: Provide a fluorescence detection device for the production of polygonatum sibiricum concentrated enzyme to prevent test tube breakage, including a fluorescence detection mechanism, and several breakage positioning mechanisms are arranged inside the fluorescence detection mechanism; The fluorescence detection mechanism includes: A breakage trigger component, and a fluorescent agent storage component is arranged at the upper end of the breakage trigger component; Each of the several breakage positioning mechanisms includes: A positioning valve component, and a fluorescent powder extrusion component is arranged inside the positioning valve component.

[0005] Furthermore, the breakage trigger component includes: An outer protective cover, a cover is provided at the upper end of the outer protective cover, the lower end of the cover is fixedly connected with a middle protective cover, a plurality of mounting holes are provided on the middle protective cover, the middle protective cover is coaxially arranged with the outer protective cover, the bottom of the outer protective cover is fixedly connected with an inner protective cover, a plurality of connection holes are provided on the inner protective cover, the inner protective cover is coaxially arranged with the outer protective cover, a circular plate is slidably connected between the outer protective cover and the middle protective cover, a first spring is connected between the circular plate and the lower end of the cover, the test tube is coaxially arranged with the inner protective cover, and a rubber stopper is connected to the opening of the test tube.

[0006] Further, the fluorescent agent storage assembly includes: A push rod, the lower end of the push rod is fixedly connected with the circular plate, the upper end of the push rod is fixedly connected with a piston, the upper end of the cover is fixedly connected with a storage tube, the piston is slidably connected in the storage tube, a fluorescent agent outlet and a fluorescent agent inlet are provided at the upper end of the storage tube, the fluorescent agent outlet is fixedly connected with one end of a rubber tube, and the other end of the rubber tube extends into the test tube through the piston.

[0007] Further, the positioning valve assembly includes: An outer valve body, the outer valve body is fixedly connected in the mounting hole, an inner valve body is fixedly connected in the outer valve body, a valve core is slidably connected in the inner valve body, one end of the valve core is conical, the valve core is connected with the outer valve body through a second spring, one end of the outer valve body is fixedly connected with a spray head, a fluorescent powder outlet is provided at a position close to the edge of the spray head, and the axial center position of the spray head extends into the connection hole.

[0008] Further, the fluorescent powder extrusion assembly includes: A first ring, a plurality of triangular blocks are fixedly connected to one side of the first ring, the first ring is fixedly connected with a second ring, the first ring is slidably connected in the cavity between the inner valve body and the outer valve body, a cavity formed by the left side of the second ring, the inner valve body and the spray head is filled with fluorescent powder, the inclined surface of the triangular block abuts against one end of a push rod, a third spring is sleeved on the push rod, the push rod is slidably connected to the inner valve body, and the other end of the push rod abuts against one end of the valve core.

[0009] Compared with the prior art, the above one or more technical solutions have the following beneficial effects: 1. When the annular plate moves upward, the air pressure in the cavity formed by the annular plate, the inner protective cover, the middle protective cover and the outer protective cover decreases, causing the valve core to move to the left under the pull of spring 2. When the valve core moves to the left, it pushes the push rod to move through the tapered part of the valve core. The other end of the push rod pushes the triangular block to move horizontally, thereby causing ring 1 to drive ring 2 to move to the right, squeezing out the fluorescent powder. At the same time, the nozzle generates suction on the connecting hole, making it easier for the liquid in the test tube to flow out of the crack. After being squeezed out, the fluorescent powder scatters to the position of the connecting hole. The liquid seeps out of the connecting hole, generating a fluorescent reaction, thereby locating the crack. The change in air pressure triggers the injection of fluorescent agent into the test tube to quickly determine whether it is damaged. The fluorescent powder is sprayed to the crack on the outer wall of the test tube through the valve assembly. Combined with the fluorescent reaction of the seepage, the damage is located, significantly improving the detection accuracy. 2. When the test tube is damaged, the air pressure inside the test tube decreases, causing the balance to be destroyed, resulting in the reset of spring 1. Under the action of spring 1, the annular plate moves upward. When the test tube is subjected to external impact, the annular plate slides between the outer protective cover and the middle protective cover. Spring 1 is compressed to absorb vibration energy and reduce the force transmitted to the test tube. When the annular plate moves upward, the push rod causes the piston to squeeze out the fluorescent agent in the storage tube, allowing the fluorescent agent to enter the test tube. Then, by observing the fluorescent reaction in the test tube, it is determined whether the test tube is damaged. When the test tube is damaged, the internal negative pressure triggers the reset of spring 1, and the push rod is linked to extrude the fluorescent agent to achieve rapid response. The triple protective cover structure absorbs impact energy through the sliding structure and spring buffering, thereby reducing the vibration transmission efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0011] Furthermore, the terms "installed," "disposed," "provided with," "connected," "connected," and "socketed" should be interpreted broadly. For example, they can refer to fixed connections, removable connections, or integral structures; mechanical connections or electrical connections; direct connections, indirect connections through an intermediary, or internal communication between two devices, elements, or components. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.

[0012] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention; Figure 2 This is a cross-sectional view of the overall three-dimensional structure of the damage trigger assembly of the present invention; Figure 3 This is a schematic diagram of the overall three-dimensional structure of the fluorescent agent storage assembly of the present invention; Figure 4Schematic diagram of the overall three-dimensional structure of the middle protective cover of the present invention; Figure 5 Front view of the overall three-dimensional structure of the middle protective cover of the present invention; Figure 6 Schematic diagram of the overall three-dimensional structure of the inner protective cover of the present invention; Figure 7 Schematic diagram of the overall three-dimensional structure of the damage positioning mechanism of the present invention; Figure 8 Cross-sectional view of the overall three-dimensional structure of the damage positioning mechanism of the present invention; Figure 9 Exploded schematic diagram of the overall three-dimensional structure of the damage positioning mechanism of the present invention; Figure 10 Schematic diagram of the overall three-dimensional structure of the phosphor extrusion assembly of the present invention; In the drawings, the list of components represented by each reference numeral is as follows: 1. Fluorescence detection mechanism; 11. Damage trigger assembly; 111. Outer protective cover; 112. Sealing cover; 113. Middle protective cover; 114. Mounting hole; 115. Inner protective cover; 116. Connecting hole; 117. Annular plate; 118. Spring 1; 12. Fluorescent agent storage assembly; 121. Push rod; 122. Piston; 123. Storage tube; 124. Fluorescent agent outlet; 125. Fluorescent agent inlet; 2. Damage positioning mechanism; 21. Positioning valve assembly; 211. Outer valve body; 212. Inner valve body; 213. Valve core; 214. Spring 2; 215. Sprayer; 216. Phosphor outlet; 22. Phosphor extrusion assembly; 221. Ring 1; 222. Triangular block; 223. Ring 2; 224. Thumb rod; 225. Spring 3. Detailed implementation manners

[0013] In order to enable those skilled in the art to better understand the solution of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0014] Refer to Figure 1 and 7 As shown in FIGS. -9, a fluorescence detection device for the production of polygonatum concentrated enzyme to prevent test tube breakage includes a fluorescence detection mechanism 1, and a plurality of damage positioning mechanisms 2 are provided in the fluorescence detection mechanism 1; The fluorescence detection mechanism 1 includes: A damage trigger assembly 11, and a fluorescent agent storage assembly 12 is provided at the upper end of the damage trigger assembly 11; Each of the plurality of damage positioning mechanisms 2 includes: A positioning valve assembly 21, and a phosphor extrusion assembly 22 is provided inside the positioning valve assembly 21.

[0015] When the test tube is damaged, the air pressure inside the test tube decreases, causing the damage trigger assembly 11 to trigger and push the fluorescent agent storage assembly 12 to squeeze the fluorescent agent inside it into the test tube, generating a fluorescence reaction. At the same time, the positioning valve assembly 21 pushes the phosphor extrusion assembly 22 to extrude the phosphor onto the outer wall of the test tube. Observe the fluorescence reaction on the outer wall of the test tube. The position where the fluorescence reaction appears is the damaged position.

[0016] Refer to Figure 2 and 4 As shown in Fig. -6, the damage trigger assembly 11 includes: An outer protective cover 111, with a cover 112 provided at the upper end of the outer protective cover 111. A middle protective cover 113 is fixedly connected to the lower end of the cover 112. A plurality of mounting holes 114 are provided on the middle protective cover 113. The middle protective cover 113 is coaxially arranged with the outer protective cover 111. The bottom of the outer protective cover 111 is fixedly connected to an inner protective cover 115. A plurality of connection holes 116 are provided on the inner protective cover 115. The inner protective cover 115 is coaxially arranged with the outer protective cover 111. An annular plate 117 is slidably connected between the outer protective cover 111 and the middle protective cover 113. A first spring 118 is connected between the annular plate 117 and the lower end of the cover 112. The test tube is coaxially arranged with the inner protective cover 115, and a rubber stopper is connected to the opening of the test tube.

[0017] When the test tube is damaged, the air pressure inside the test tube decreases, causing the balance to be disrupted, resulting in the reset of the first spring 118. Under the action of the first spring 118, the annular plate 117 moves upward. When the test tube is subjected to an external impact, the annular plate 117 slides between the outer protective cover 111 and the middle protective cover 113, and the first spring 118 compresses to absorb the vibration energy and reduce the force transmitted to the test tube.

[0018] Refer to Figure 3 As shown in the figure, the fluorescent agent storage assembly 12 includes: A push rod 121, the lower end of the push rod 121 is fixedly connected to the annular plate 117, the upper end of the push rod 121 is fixedly connected to a piston 122. The upper end of the cover 112 is fixedly connected to a storage tube 123. The piston 122 is slidably connected inside the storage tube 123. The upper end of the storage tube 123 is provided with a fluorescent agent outlet 124 and a fluorescent agent inlet 125. The fluorescent agent outlet 124 is fixedly connected to one end of a rubber tube, and the other end of the rubber tube extends into the test tube through the piston 122.

[0019] When the annular plate 117 moves upward, under the action of the push rod 121, the piston 122 extrudes the fluorescent agent in the storage tube 123, so that the fluorescent agent enters the test tube. Then, by observing the fluorescence reaction in the test tube, it can be judged whether the test tube is damaged.

[0020] The positioning valve assembly 21 includes: An outer valve body 211, the outer valve body 211 is fixedly connected in the mounting hole 114. An inner valve body 212 is fixedly connected inside the outer valve body 211. A valve core 213 is slidably connected inside the inner valve body 212. One end of the valve core 213 is conical. The valve core 213 is connected to the outer valve body 211 through a second spring 214. One end of the outer valve body 211 is fixedly connected with a nozzle 215. A fluorescent powder outlet 216 is provided at a position near the edge of the nozzle 215. The axial center position of the nozzle 215 extends into the connection hole 116.

[0021] When the annular plate 117 moves upward, the air pressure in the cavity formed by the annular plate 117, the inner protective cover 115, the middle protective cover 113 and the outer protective cover 111 decreases, so that the valve core 213 moves leftward under the pull of the second spring 214.

[0022] Refer to Figure 10 As shown, the fluorescent powder extrusion assembly 22 includes: A first ring 221. A plurality of triangular blocks 222 are fixedly connected to one side of the first ring 221. The first ring 221 is fixedly connected to a second ring 223. The first ring 221 is slidably connected in the cavity between the inner valve body 212 and the outer valve body 211. Fluorescent powder is provided in the cavity formed by the left side of the second ring 223, the inner valve body 212 and the nozzle 215. The inclined surface of the triangular block 222 abuts against one end of the ejector rod 224. A third spring 225 is sleeved on the ejector rod 224. The ejector rod 224 is slidably connected to the inner valve body 212. The other end of the ejector rod 224 abuts against one end of the valve core 213.

[0023] When the valve core 213 moves leftward, it pushes the ejector rod 224 to move through the conical part of the valve core 213. The other end of the ejector rod 224 pushes the triangular block 222 to move horizontally. Then, the first ring 221 drives the second ring 223 to move rightward to extrude the fluorescent powder. At the same time, the nozzle 215 generates a suction force at the connection hole 116, making it easier for the liquid in the test tube to flow out from the crack. After the fluorescent powder is extruded, it scatters to the position of the connection hole 116, and the liquid seeps out from the connection hole 116 to produce a fluorescence reaction, thereby positioning the crack.

[0024] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationships shown in the drawings. These are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present invention. In the description of the present invention, unless otherwise specified and defined, it should be noted that the terms "mounted", "connected", and "coupled" should be understood in a broad sense. For example, it may be a mechanical connection or an electrical connection, or it may be the communication inside two elements. It may be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms can be understood according to specific circumstances.

[0025] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A fluorescence detection device for the production of polygonatum sibiricum red juice concentrate to prevent test tube breakage, characterized in that: It includes a fluorescence detection mechanism (1), and several breakage positioning mechanisms (2) are arranged inside the fluorescence detection mechanism (1); The fluorescence detection mechanism (1) includes: A breakage trigger component (11), and a fluorescent agent storage component (12) is arranged at the upper end of the breakage trigger component (11); Each of the several breakage positioning mechanisms (2) includes: A positioning valve component (21), and a fluorescent powder extrusion component (22) is arranged inside the positioning valve component (21).

2. The fluorescence detection device for the production of polygonatum sibiricum red juice concentrate to prevent test tube breakage according to claim 1, characterized in that: The breakage trigger component (11) includes: An outer protective cover (111), a cover (112) is arranged at the upper end of the outer protective cover (111), the lower end of the cover (112) is fixedly connected with a middle protective cover (113), several mounting holes (114) are opened on the middle protective cover (113), the middle protective cover (113) is coaxially arranged with the outer protective cover (111), the bottom of the outer protective cover (111) is fixedly connected with an inner protective cover (115), several connection holes (116) are opened on the inner protective cover (115), the inner protective cover (115) is coaxially arranged with the outer protective cover (111), an annular plate (117) is slidably connected between the outer protective cover (111) and the middle protective cover (113), a first spring (118) is connected between the annular plate (117) and the lower end of the cover (112), the test tube is coaxially arranged with the inner protective cover (115), and a rubber stopper is connected to the opening of the test tube.

3. The fluorescence detection device for the production of polygonatum sibiricum red juice concentrate to prevent test tube breakage according to claim 2, characterized in that: The fluorescent agent storage component (12) includes: A push rod (121), the lower end of the push rod (121) is fixedly connected with the annular plate (117), the upper end of the push rod (121) is fixedly connected with a piston (122), the upper end of the cover (112) is fixedly connected with a storage tube (123), the piston (122) is slidably connected inside the storage tube (123), a fluorescent agent outlet (124) and a fluorescent agent inlet (125) are opened at the upper end of the storage tube (123), the fluorescent agent outlet (124) is fixedly connected with one end of a rubber tube, and the other end of the rubber tube extends into the test tube through the piston (122).

4. The fluorescence detection device for the production of polygonatum sibiricum red juice concentrate to prevent test tube breakage according to claim 3, characterized in that: The positioning valve component (21) includes: An outer valve body (211) is fixedly connected inside the mounting hole (114). An inner valve body (212) is fixedly connected inside the outer valve body (211). A valve core (213) is slidably connected inside the inner valve body (212). One end of the valve core (213) is conical. The valve core (213) is connected to the outer valve body (211) through a second spring (214). One end of the outer valve body (211) is fixedly connected to a spray head (215). A phosphor outlet (216) is provided at a position near the edge of the spray head (215). The axial center position of the spray head (215) extends into the connection hole (116).

5. The fluorescence detection device for the production of polygonatum concentrated enzyme for preventing test tube breakage according to claim 4, wherein: The phosphor extrusion assembly (22) includes: A first ring (221). A number of triangular blocks (222) are fixedly connected to one side of the first ring (221). The first ring (221) is fixedly connected to a second ring (223). The first ring (221) is slidably connected in the cavity between the inner valve body (212) and the outer valve body (211). Phosphor is provided in the cavity formed by the left side of the second ring (223), the inner valve body (212), and the spray head (215). The inclined surface of the triangular block (222) abuts against one end of a push rod (224). A third spring (225) is sleeved on the push rod (224). The push rod (224) is slidably connected to the inner valve body (212). The other end of the push rod (224) abuts against one end of the valve core (213).

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