Emblica officinalis extract composition with lipid-lowering function, preparation method and application thereof

Abstract

The present invention discloses a kind of emblica extract composition with blood lipid lowering function and its preparation method and application, described composition is by weight, and described composition includes the following components: emblica fruit extract 20~30 parts, mixed extract 10~15 parts, houttuynia cordata stem extract 5~10 parts, modified dietary fiber 10~20 parts, hawthorn 2~5 parts, kudzu root powder 4~8 parts, beta-carotene 0.1~0.3 parts.The composition containing emblica extract provided by the present invention, by carrying out compounding by emblica extract, mulberry leaf extract, tangerine peel extract and houttuynia cordata extract, polysaccharide, phenols, flavonoids in extract have the effect of lowering blood lipid, while adding modified dietary fiber, not only can further enhance its blood lipid lowering effect, simultaneously can form hydrogen bond with phenolic compound, interact, improve water solubility and bioaccessibility of composition, by adding hawthorn and beta-carotene, not only can improve mouthfeel, but also can further enhance blood lipid lowering effect.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and in particular to a phyllanthus emblica extract composition with blood lipid lowering function, and a preparation method and application thereof. Background Art

[0002] Dyslipidemia, commonly known as hyperlipidemia, is a chronic disease caused by impaired lipid metabolism, resulting in high blood lipid levels. It can lead to cardiovascular diseases such as atherosclerosis, coronary heart disease, and myocardial infarction. The "Dietary Guidelines for Adults with Hyperlipidemia (2023 Edition)" issued by my country's National Health Commission divides dyslipidemia into four types based on fasting venous serum test indicators: hypercholesterolemia (TC content ≥5.2 mmol / L); hypertriglyceridemia (TG content ≥1.7 mmol / L); low high-density lipoprotein cholesterol (HDL-C content <1.0 mmol / L); and high low-density lipoprotein cholesterol (LDL-C content ≥3.4 mmol / L).

[0003] Generally speaking, lipid-lowering drugs are widely used in clinical practice, especially statins. While these drugs can effectively lower blood lipids, they also have side effects, particularly potential damage to liver function and muscles. In contrast, functional foods with lipid-lowering properties offer significant advantages in long-term lipid management.

[0004] Emblica officinalis is the dried, mature fruit of the Euphorbiaceae plant, Phyllanthus emblica. Numerous ancient herbal texts document the various benefits of Emblica officinalis. For example, the Newly Revised Compendium of Materia Medica states that Emblica officinalis treats wind-deficiency and heat. The Supplement to Compendium of Materia Medica states that Emblica officinalis is beneficial for tonifying and strengthening the body's energy. The Shaoxing Compendium of Materia Medica states that the fruit of Emblica officinalis can be consumed as a detoxifier for alcohol. The Compendium of Materia Medica states that Emblica officinalis has a cooling nature, a sweet, sour, and astringent taste, and possesses the effects of clearing heat and cooling blood, promoting digestion and strengthening the stomach, and promoting fluid production and relieving coughs. Research has shown that Emblica officinalis is rich in bioactive substances such as phenols, vitamins, amino acids, and flavonoids. Modern pharmacological research also demonstrates that Emblica officinalis possesses diverse pharmacological effects, including antimicrobial, anti-tumor, lipid- and blood-sugar-lowering, antihypertensive, and tonic properties, without significant toxic side effects. For example, Chinese patent (CN113786458A) discloses a medicinal formula for lowering blood lipids, reducing blood viscosity, and softening blood vessels. The formula includes emblica fructus, wolfberry, baxiaga, angelica root, kudzu root, ganqingqinglan, cilantro, rabbit ear grass, Zhaxun paste, Chuanxiong rhizome, meconopsis, astragalus, pterygium wilfordii, safflower, dalbergia odorifera, madder, costus root, tea tree root, lithospermum officinale, dendrobium, lithospermum officinale, saxifraga serrata, terminalia chebula, hawthorn leaf, terminalia chebula, polygonatum, gastrodia elata, melon seeds, costus root, Rubus chinensis, kudzuvine root, costus root, sea buckthorn paste, bezoar, ligado, and mulberry leaf. The patent, combining traditional Chinese and Tibetan medicinal ingredients, demonstrates excellent therapeutic and preventive effects for hyperlipidemia, with mild efficacy and minimal side effects. However, the patent uses a combination of multiple traditional Chinese and Tibetan medicinal herbs, making the preparation complex. Furthermore, the effects of the various medicinal ingredients may interact with each other, making the lipid-lowering effect unpredictable. Therefore, it is crucial to develop safe and effective lipid-lowering compositions from food or edible medicinal ingredients to improve public health. Summary of the Invention

[0005] The main purpose of the present invention is to provide a composition of emblica extract with lipid-lowering function, a preparation method and application thereof, and to provide a composition developed from medicinal and edible raw materials that can safely and effectively lower blood lipids.

[0006] To achieve the above object, the present invention provides a composition containing an emblica bud extract. The composition comprises the following components, in parts by weight: 20-30 parts of emblica bud extract, 10-15 parts of a mixed extract, 5-10 parts of a houttuynia cordata stem extract, 10-20 parts of modified dietary fiber, 2-5 parts of hawthorn, 4-8 parts of kudzu root powder, and 0.1-0.3 parts of beta-carotene.

[0007] Preferably, the particle size of the kudzu root powder is 40-120 mesh.

[0008] Preferably, the mixed extract comprises a mixed extract of mulberry leaves, tangerine peel and houttuynia leaves, and the mass ratio of the mulberry leaves, tangerine peel and houttuynia leaves is (6-10): (2-4): (3-6).

[0009] Preferably, the preparation method of the modified dietary fiber comprises the following steps:

[0010] The skin and core of the sea buckthorn are peeled, washed, dried, crushed, and sieved to obtain sea buckthorn meat powder, deionized water is added, the mixture is heated to 60-80°C, stirred, centrifuged, and the supernatant is retained. The residue is washed with deionized water, vacuum-dried, crushed, and sieved again. Deionized water and cellulase are then added, and the mixture is heated in a water bath to 40-50°C for enzymolysis. After the enzymolysis is completed, the enzyme is inactivated, filtered, and a supernatant is obtained. The supernatants are combined and concentrated, and then a guar gum solution is added, and a homogenized shearing process is performed, and then freeze-dried to obtain the modified dietary fiber.

[0011] Preferably, the sieved filter is 50-100 mesh, and the second sieved filter is 200-300 mesh;

[0012] The mass fraction of the cellulase is 0.2-0.4%;

[0013] The amount of the cellulase is 0.1-0.4% of the mass of the roxburghii;

[0014] The amount of the guar gum solution is 1-2% of the mass of the roxburghii;

[0015] The speed of the homogenizing shearing process is 8000-10000 rpm;

[0016] The homogenization shearing treatment time is 4 to 6 minutes;

[0017] The freeze-drying temperature is -20 to -30°C.

[0018] The present invention also provides a method for preparing the composition containing the emblica extract as described above, comprising the following steps:

[0019] S1. Wash and remove the core of fresh emblica fruit, then crush the cored pulp and peel together with a wall-breaking machine, then microwave-treat, add an ethanol solution, stir evenly, then add glycine, heat and extract for a period of time, filter, and concentrate the filtrate to obtain the emblica fruit extract;

[0020] S2, mulberry leaf, dried tangerine peel and houttuynia leaf are washed respectively, then crushed, sieved, and mixed in proportion to obtain a mixture, a complex enzyme is added, and enzymolysis is carried out under ultrasonic conditions. After the enzymolysis is complete, the enzyme is inactivated, and a methanol solution is added to the mixture for extraction, filtered, chitosan is added to the filtrate, stirred, and then methanol is recovered and concentrated under reduced pressure to obtain a mixed extract;

[0021] S3, washing and crushing the stems of Houttuynia cordata, sieving, adding deionized water, stirring, then adding ethanol solution and citric acid solution, extracting under heating conditions, filtering, and evaporating and concentrating to obtain a stem extract of Houttuynia cordata;

[0022] S4. Wash and remove the core of the hawthorn, crush it, and sieve it to obtain hawthorn powder. Then, mix the hawthorn powder, kudzu root powder, the emblica fruit extract, the mixed extract, the houttuynia cordata stem extract, and the modified dietary fiber in proportion, and add β-carotene in a corresponding proportion to obtain a composition containing the emblica fruit extract.

[0023] Preferably, in step S1, the power of the microwave treatment is 550-650 W, and the time of the microwave treatment is 3-5 min;

[0024] The heating extraction temperature is 50-60° C., and the extraction time is 5-10 min.

[0025] Preferably, in step S1, the mass ratio of glycine to emblica fruit is (1-3): (12-15).

[0026] Preferably, in step S2, the mass fraction of the methanol solution is 60-80%;

[0027] The power of the ultrasound is 500-600W;

[0028] The extraction temperature is 40-50°C;

[0029] The complex enzyme is a mixed enzyme of cellulase and pectinase;

[0030] The mass ratio of the chitosan to the mulberry leaves is (1-3): (550-600).

[0031] Preferably, in step S3, the mass fraction of the ethanol solution is 60-80%;

[0032] The mass fraction of the citric acid solution is 20-40%;

[0033] The heating temperature is 50-70°C.

[0034] The present invention further proposes the use of the composition containing the emblica extract as described above in functional foods for lowering blood lipids.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) The composition containing the emblica extract provided by the present invention is prepared by compounding the emblica extract, tangerine peel extract, mulberry leaf extract and houttuynia cordata extract. The polysaccharides, phenols and flavonoids in the extract have the effect of lowering blood lipids. The addition of modified dietary fiber can not only further enhance the blood lipid-lowering effect, but also form hydrogen bonds with the phenolic compounds to interact with each other, thereby improving the water solubility and bioaccessibility of the composition. The addition of hawthorn can not only improve the taste, but also further enhance the blood lipid-lowering effect.

[0037] (2) The composition containing the extract of Phyllanthus amla provided by the present invention adopts different extraction processes according to the characteristics of each raw material during the extraction process, and the extraction efficiency is high. For the extraction of Phyllanthus amla fruit, the plant cell wall is first destroyed by a wall breaker to increase the contact area between the cell and the solvent, thereby improving the extraction efficiency of the active ingredient. Then, microwave-assisted solvent extraction is used to promote the faster release of the components in the cell. At the same time, glycine is added, which is equivalent to providing a hydrogen bond acceptor, forming a strong hydrogen bond network with the hydrogen bond donor provided by the phenols in the system, thereby increasing the solubility of the entire reaction system and greatly improving the extraction efficiency. For the extraction of mulberry leaves, tangerine peel and Houttuynia cordata leaves, the main components of their cell walls are cellulose and pectin. The method of enzymatic hydrothermal solvent extraction is used to quickly destroy the cell wall, release more active ingredients, and improve the extraction efficiency. At the same time, the natural polysaccharide chitosan is added, which can not only enhance the compatibility and biological activity of the various components, but also prolong the shelf life of the composition. For the extraction of Houttuynia cordata stem, organic acid citric acid is added to form an acidic environment, and the solvent extraction method is used to increase the active ingredients in the Houttuynia cordata stem.

[0038] (3) The composition containing the emblica extract provided by the present invention can inhibit the synthesis of cholesterol and promote the metabolism of cholesterol, thereby reducing the total cholesterol level (TC) in the blood. At the same time, it can significantly reduce the concentrations of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly increase the concentration of high-density lipoprotein cholesterol (HDL-C), thereby reducing blood lipids and treating hyperlipidemia. Moreover, the main ingredients of the composition provided by the present invention, namely, the emblica extract, mulberry leaf extract, tangerine peel extract, and houttuynia cordata extract, are all derived from substances that are both medicinal and edible. The substances extracted by different methods are mixed in a specific proportion, which has less irritation to the gastrointestinal mucosa and will not produce significant side effects on the consumer even if consumed for a long time. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0040] Figure 1 The present invention is a schematic flow chart of an embodiment of a method for preparing a composition containing an extract of Emblica officinalis.

[0041] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. If specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. If the reagents or instruments used are not specified by the manufacturer, they are all conventional products that can be purchased commercially. In addition, the technical solutions between the various embodiments can be combined with each other, but must be based on the ability of ordinary technicians in this field to achieve. When the combination of technical solutions is mutually contradictory or cannot be achieved, it should be considered that the combination of such technical solutions does not exist and is not within the scope of protection required by the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0043] The technical solutions of the present invention are further described in detail below in conjunction with specific embodiments and drawings. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0044] Example 1

[0045] A composition containing emblica bud extract comprises the following components: 25g of emblica bud extract, 12g of mixed extract, 7g of houttuynia cordata stem extract, 15g of modified dietary fiber, 3g of hawthorn, 6g of kudzu root powder, and 0.2g of beta-carotene.

[0046] The preparation method of the modified dietary fiber comprises the following steps:

[0047] After peeling and coreing the roxburghii, 150 g of roxburghii flesh was weighed, washed, dried, crushed, and passed through a 60-mesh sieve to obtain roxburghii flesh powder, added with 2000 mL of deionized water, heated to 70 ° C, stirred, and centrifuged for 30 min. The supernatant was retained, and the residue was washed twice with deionized water, vacuum-dried, crushed, and passed through a 250-mesh sieve again. Then, 1000 mL of deionized water and 0.3 g of cellulase were added, and the mixture was heated to 45 ° C in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 1.8 g of guar gum solution was added. The mixture was homogenized and sheared at 9000 rpm for 5 min, and then freeze-dried at -25 ° C to obtain the modified dietary fiber.

[0048] The preparation method of the composition containing the emblica extract comprises the following steps:

[0049] (1) 250 g of fresh emblica fruit was washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 4 min. A 60% ethanol solution was added and stirred evenly. Then 20 g of glycine was added, and the mixture was heated to 55 °C and extracted for 8 min. The mixture was filtered and the filtrate was concentrated to obtain the emblica fruit extract.

[0050] (2) 60 g of mulberry leaves, 30 g of dried tangerine peel and 50 g of houttuynia leaves were washed and crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. 1 g of cellulase and 1 g of pectinase were added to the mixture, and enzymatic hydrolysis was performed under 550 W ultrasonic conditions. After the enzymatic hydrolysis, the enzyme was inactivated. 300 mL of 70% methanol solution was added to the mixture for extraction, and the mixture was filtered. 0.2 g of chitosan was added to the filtrate, and the mixture was stirred evenly. The methanol was then recovered and concentrated under reduced pressure to obtain a mixed extract.

[0051] (3) 70 g of Houttuynia cordata stems were washed and crushed, passed through a 150-mesh sieve, added with deionized water, stirred, and then added with 70% ethanol solution and 30% citric acid solution. The mixture was heated at 60°C for extraction, filtered, and evaporated to obtain the Houttuynia cordata stem extract.

[0052] (4) 8 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of emblica fruit extract, 12 g of mixed extract, 7 g of houttuynia cordata stem extract, and 15 g of modified dietary fiber were mixed, and 0.2 g of β-carotene was added to obtain a composition containing emblica extract.

[0053] Example 2

[0054] A composition containing emblica bud extract comprises the following components: 20g of emblica bud extract, 10g of mixed extract, 5g of houttuynia cordata stem extract, 10g of modified dietary fiber, 2g of hawthorn, 4g of kudzu root powder, and 0.1g of beta-carotene.

[0055] The preparation method of the modified dietary fiber comprises the following steps:

[0056] After peeling and coreing the sea buckthorn, 100 g of sea buckthorn meat was weighed, washed, dried, crushed, and passed through a 50-mesh sieve to obtain sea buckthorn meat powder, added with 1800 mL of deionized water, heated to 60° C., stirred, and centrifuged for 30 min. The supernatant was retained, and the residue was washed twice with deionized water, vacuum-dried, crushed, and passed through a 200-mesh sieve again. Then, 800 mL of deionized water and 0.1 g of cellulase were added, and the mixture was heated to 45° C. in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 1.5 g of guar gum solution was added. The mixture was homogenized and sheared at 8000 rpm for 4 min, and then freeze-dried at -20° C. to obtain the modified dietary fiber.

[0057] The preparation method of the composition containing the emblica extract comprises the following steps:

[0058] (1) 200 g of fresh emblica fruit was washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 3 min. A 70% ethanol solution was added and stirred evenly. Then 15 g of glycine was added, and the mixture was heated to 50 °C and extracted for 10 min. The mixture was filtered and the filtrate was concentrated to obtain the emblica fruit extract.

[0059] (2) 50 g of mulberry leaves, 20 g of dried tangerine peel and 30 g of houttuynia leaves were washed and crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. A composite enzyme of 1 g of cellulase and 1 g of pectinase was added, and enzymatic hydrolysis was performed under 550 W ultrasonic conditions. After the enzymatic hydrolysis, the enzyme was inactivated. Then, 300 mL of 70% methanol solution was added to the mixture for extraction, and the mixture was filtered. 0.2 g of chitosan was added to the filtrate, and the mixture was stirred evenly. Then, the methanol was recovered and concentrated under reduced pressure to obtain a mixed extract.

[0060] (3) 50 g of Houttuynia cordata stems were washed and crushed, passed through a 100-mesh sieve, added with deionized water, stirred, and then added with 60% ethanol solution and 30% citric acid solution by mass. The mixture was heated at 50°C for extraction, filtered, and evaporated to obtain the Houttuynia cordata stem extract.

[0061] (4) 5 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 2 g of hawthorn powder, 4 g of kudzu root powder, 20 g of emblica fruit extract, 10 g of mixed extract, 5 g of houttuynia cordata stem extract, and 10 g of modified dietary fiber were mixed, and 0.1 g of β-carotene was added to obtain a composition containing emblica extract.

[0062] Example 3

[0063] A composition containing emblica bud extract comprises the following components: 30g of emblica bud extract, 15g of mixed extract, 10g of houttuynia cordata stem extract, 20g of modified dietary fiber, 5g of hawthorn, 5g of kudzu root powder, and 0.3g of beta-carotene.

[0064] The preparation method of the modified dietary fiber comprises the following steps:

[0065] After peeling and coreing the roxburghii, 200 g of roxburghii flesh was weighed, washed, dried, and crushed, and passed through a 100-mesh sieve to obtain roxburghii flesh powder. 2200 mL of deionized water was added, and the mixture was heated to 80° C., stirred, and centrifuged for 30 min. The supernatant was retained. The residue was washed three times with deionized water, vacuum-dried, crushed, and passed through a 300-mesh sieve again. Then, 1200 mL of deionized water and 0.4 g of cellulase were added, and the mixture was heated to 45° C. in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 2 g of guar gum solution was added. The mixture was homogenized and sheared at 10,000 rpm for 6 min, and then freeze-dried at -30° C. to obtain the modified dietary fiber.

[0066] The preparation method of the composition containing the emblica extract comprises the following steps:

[0067] (1) 300 g of fresh emblica fruit were washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 5 min. A 65% ethanol solution was added and stirred evenly. Then 25 g of glycine was added, and the mixture was heated to 60 °C and extracted for 5 min. The mixture was filtered and the filtrate was concentrated to obtain the emblica fruit extract.

[0068] (2) 100 g of mulberry leaves, 40 g of dried tangerine peel and 60 g of houttuynia leaves were washed separately, then crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. A complex enzyme of 1 g of cellulase and 0.8 g of pectinase was added, and enzymatic hydrolysis was carried out under 600 W ultrasonic conditions. After the enzymatic hydrolysis was completed, the enzyme was inactivated, and then 200 mL of 80% methanol solution was added to the mixture for extraction. The mixture was filtered, and 0.4 g of chitosan was added to the filtrate. The mixture was stirred evenly, and then the methanol was recovered and concentrated under reduced pressure to obtain a mixed extract.

[0069] (3) 100 g of Houttuynia cordata stems were washed and crushed, passed through a 200-mesh sieve, added with deionized water, stirred, and then added with 80% ethanol solution and 30% citric acid solution by mass, and extracted under heating conditions of 70°C, filtered, and evaporated to obtain the Houttuynia cordata stem extract;

[0070] (4) 10 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 5 g of hawthorn powder, 5 g of kudzu root powder, 30 g of emblica fruit extract, 15 g of mixed extract, 10 g of houttuynia cordata stem extract, and 20 g of modified dietary fiber were mixed, and 0.3 g of β-carotene was added to obtain a composition containing emblica extract.

[0071] Example 4

[0072] A composition containing emblica bud extract comprises the following components: 25g of emblica bud extract, 12g of mixed extract, 7g of houttuynia cordata stem extract, 15g of modified dietary fiber, 3g of hawthorn, 6g of kudzu root powder, and 0.2g of beta-carotene.

[0073] The preparation method of the modified dietary fiber comprises the following steps:

[0074] After peeling and coreing the roxburghii, 150 g of roxburghii flesh was weighed, washed, dried, crushed, and passed through a 60-mesh sieve to obtain roxburghii flesh powder, added with 2000 mL of deionized water, heated to 70 ° C, stirred, and centrifuged for 30 min. The supernatant was retained, and the residue was washed twice with deionized water, vacuum-dried, crushed, and passed through a 250-mesh sieve again. Then, 1000 mL of deionized water and 0.3 g of cellulase were added, and the mixture was heated to 45 ° C in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 1.8 g of guar gum solution was added. The mixture was homogenized and sheared at 9000 rpm for 5 min, and then freeze-dried at -25 ° C to obtain the modified dietary fiber.

[0075] The preparation method of the composition containing the emblica extract comprises the following steps:

[0076] (1) 250 g of fresh emblica fruit was washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 4 min. A 60% ethanol solution was added, stirred evenly, heated to 55 °C for 8 min, filtered, and the filtrate was concentrated to obtain the emblica fruit extract.

[0077] (2) 60 g of mulberry leaves, 30 g of dried tangerine peel and 50 g of houttuynia leaves were washed and crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. A complex enzyme of 1 g of cellulase and 1 g of pectinase was added, and enzymatic hydrolysis was performed under 550 W ultrasonic conditions. After the enzymatic hydrolysis was completed, the enzyme was inactivated, and then 300 mL of 70% methanol solution was added to the mixture for extraction. The mixture was filtered, and then the methanol was recovered and concentrated under reduced pressure to obtain a mixed extract.

[0078] (3) 70 g of Houttuynia cordata stems were washed and crushed, passed through a 150-mesh sieve, added with deionized water, stirred, and then added with 70% ethanol solution and 30% citric acid solution. The mixture was heated at 60°C for extraction, filtered, and evaporated to obtain the Houttuynia cordata stem extract.

[0079] (4) 8 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of emblica fruit extract, 12 g of mixed extract, 7 g of houttuynia cordata stem extract, and 15 g of modified dietary fiber were mixed, and 0.2 g of β-carotene was added to obtain a composition containing emblica extract.

[0080] Comparative Example 1

[0081] A composition containing emblica bud extract comprises the following components: 25g emblica bud extract, 12g mixed extract, 7g houttuynia cordata stem extract, 3g hawthorn, 6g kudzu root powder and 0.2g beta-carotene.

[0082] The preparation method of the composition containing the emblica extract comprises the following steps:

[0083] (1) 250 g of fresh emblica fruit was washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 4 min. A 60% ethanol solution was added and stirred evenly. Then 20 g of glycine was added, and the mixture was heated to 55 °C and extracted for 8 min. The mixture was filtered and the filtrate was concentrated to obtain the emblica fruit extract.

[0084] (2) 60 g of mulberry leaves, 30 g of dried tangerine peel and 50 g of houttuynia leaves were washed and crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. 1 g of cellulase and 1 g of pectinase were added to the mixture, and enzymatic hydrolysis was performed under 550 W ultrasonic conditions. After the enzymatic hydrolysis, the enzyme was inactivated. 300 mL of 70% methanol solution was added to the mixture for extraction, and the mixture was filtered. 0.2 g of chitosan was added to the filtrate, and the mixture was stirred evenly. The methanol was then recovered and concentrated under reduced pressure to obtain a mixed extract.

[0085] (3) 70 g of Houttuynia cordata stems were washed and crushed, passed through a 150-mesh sieve, added with deionized water, stirred, and then added with 70% ethanol solution and 30% citric acid solution. The mixture was heated at 60°C for extraction, filtered, and evaporated to obtain the Houttuynia cordata stem extract.

[0086] (4) 8 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of emblica fruit extract, 12 g of mixed extract, and 7 g of houttuynia cordata stem extract were mixed, and 0.2 g of β-carotene was added to obtain a composition containing emblica extract.

[0087] Comparative Example 2

[0088] A composition containing emblica bud extract comprises the following components: 25g of emblica bud extract, 7g of houttuynia cordata stem extract, 15g of modified dietary fiber, 3g of hawthorn, 6g of kudzu root powder, and 0.2g of beta-carotene.

[0089] The preparation method of the modified dietary fiber comprises the following steps:

[0090] After peeling and coreing the roxburghii, 150 g of roxburghii flesh was weighed, washed, dried, and crushed, and passed through a 60-mesh sieve to obtain roxburghii flesh powder. 2000 mL of deionized water was added, and the mixture was heated to 70° C., stirred, and centrifuged for 30 min. The supernatant was retained, and the residue was washed twice with deionized water, vacuum-dried, crushed, and passed through a 250-mesh sieve again. Then, 1000 mL of deionized water and 0.3 g of cellulase were added, and the mixture was heated to 45° C. in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 1.8 g of guar gum solution was added. The mixture was homogenized and sheared at 9000 rpm for 5 min, and then freeze-dried at -25° C. to obtain the modified dietary fiber.

[0091] The preparation method of the composition containing the emblica extract comprises the following steps:

[0092] (1) 250 g of fresh emblica fruit was washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 4 min. A 60% ethanol solution was added and stirred evenly. Then 20 g of glycine was added, and the mixture was heated to 55 °C and extracted for 8 min. The mixture was filtered and the filtrate was concentrated to obtain the emblica fruit extract.

[0093] (2) 70 g of Houttuynia cordata stems were washed and crushed, passed through a 150-mesh sieve, added with deionized water, stirred, and then added with 70% ethanol solution and 30% citric acid solution by mass, and extracted under heating conditions of 60°C, filtered, and evaporated to obtain the Houttuynia cordata stem extract;

[0094] (3) 8 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of emblica fruit extract, 7 g of houttuynia cordata stem extract, and 15 g of modified dietary fiber were mixed, and 0.2 g of β-carotene was added to obtain a composition containing emblica extract.

[0095] Comparative Example 3

[0096] A composition containing emblica bud extract comprises the following components: 25g of emblica bud extract, 12g of mixed extract, 15g of modified dietary fiber, 3g of hawthorn, 6g of kudzu root powder, and 0.2g of beta-carotene.

[0097] The preparation method of the modified dietary fiber comprises the following steps:

[0098] After peeling and coreing the roxburghii, 150 g of roxburghii flesh was weighed, washed, dried, and crushed, and passed through a 60-mesh sieve to obtain roxburghii flesh powder. 2000 mL of deionized water was added, and the mixture was heated to 70° C., stirred, and centrifuged for 30 min. The supernatant was retained, and the residue was washed twice with deionized water, vacuum-dried, crushed, and passed through a 250-mesh sieve again. Then, 1000 mL of deionized water and 0.3 g of cellulase were added, and the mixture was heated to 45° C. in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 1.8 g of guar gum solution was added. The mixture was homogenized and sheared at 9000 rpm for 5 min, and then freeze-dried at -25° C. to obtain the modified dietary fiber.

[0099] The preparation method of the composition containing the emblica extract comprises the following steps:

[0100] (1) 250 g of fresh emblica fruit was washed and pitted, and the pitted pulp and peel were crushed together with a wall-breaking machine, and then microwaved for 4 min. A 60% ethanol solution was added and stirred evenly. Then 20 g of glycine was added, and the mixture was heated to 55 °C and extracted for 8 min. The mixture was filtered and the filtrate was concentrated to obtain the emblica fruit extract.

[0101] (2) 60 g of mulberry leaves, 30 g of dried tangerine peel and 50 g of houttuynia leaves were washed and crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. 1 g of cellulase and 1 g of pectinase were added to the mixture, and enzymatic hydrolysis was performed under 550 W ultrasonic conditions. After the enzymatic hydrolysis, the enzyme was inactivated. 300 mL of 70% methanol solution was added to the mixture for extraction, and the mixture was filtered. 0.2 g of chitosan was added to the filtrate, and the mixture was stirred evenly. The methanol was then recovered and concentrated under reduced pressure to obtain a mixed extract.

[0102] (3) 8 g of hawthorn was washed, pitted, crushed, and sieved to obtain hawthorn powder. 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of emblica fruit extract, 12 g of mixed extract, and 15 g of modified dietary fiber were mixed, and 0.2 g of β-carotene was added to obtain a composition containing emblica fruit extract.

[0103] Comparative Example 4

[0104] A composition comprises the following components: 12g of mixed extract, 7g of houttuynia cordata stem extract, 15g of modified dietary fiber, 3g of hawthorn, 6g of kudzu root powder, and 0.2g of beta-carotene.

[0105] The preparation method of the modified dietary fiber comprises the following steps:

[0106] After peeling and coreing the roxburghii, 150 g of roxburghii flesh was weighed, washed, dried, and crushed, and passed through a 60-mesh sieve to obtain roxburghii flesh powder. 2000 mL of deionized water was added, and the mixture was heated to 70° C., stirred, and centrifuged for 30 min. The supernatant was retained, and the residue was washed twice with deionized water, vacuum-dried, crushed, and passed through a 250-mesh sieve again. Then, 1000 mL of deionized water and 0.3 g of cellulase were added, and the mixture was heated to 45° C. in a water bath for enzymolysis. After the enzymolysis was complete, the enzyme was inactivated and filtered to obtain a supernatant. The supernatants were combined and concentrated, and then 1.8 g of guar gum solution was added. The mixture was homogenized and sheared at 9000 rpm for 5 min, and then freeze-dried at -25° C. to obtain the modified dietary fiber.

[0107] The preparation method of the composition comprises the following steps:

[0108] (1) 60 g of mulberry leaves, 30 g of dried tangerine peel and 50 g of houttuynia leaves were washed and crushed, passed through a 100-mesh sieve, and mixed in proportion to obtain a mixture. 1 g of cellulase and 1 g of pectinase were added, and enzymatic hydrolysis was performed under 550 W ultrasonic conditions. After the enzymatic hydrolysis was completed, the enzyme was inactivated, and then 300 mL of 70% methanol solution was added to the mixture for extraction. The mixture was filtered, and 0.2 g of chitosan was added to the filtrate. The methanol was then recovered and concentrated under reduced pressure to obtain a mixed extract.

[0109] (2) 70 g of Houttuynia cordata stems were washed and crushed, passed through a 150-mesh sieve, added with deionized water, stirred, and then added with 70% ethanol solution and 30% citric acid solution by mass, and extracted under heating conditions of 60°C, filtered, and evaporated to obtain the Houttuynia cordata stem extract;

[0110] (3) 8 g of hawthorn was washed, cored, and crushed, sieved, and the hawthorn powder was obtained. Then, 3 g of hawthorn powder, 6 g of kudzu root powder, 12 g of mixed extract, 7 g of houttuynia cordata stem extract, and 15 g of modified dietary fiber were mixed in proportion, and 0.2 g of β-carotene was added to obtain a composition.

[0111] Test methods and results

[0112] (1) Experimental materials

[0113] Experimental animals:

[0114] 110 SPF-grade healthy adult female Wistar rats were purchased from Beijing Weitonglihua Laboratory Animal Technology Co., Ltd.

[0115] Mouse age: 50 days

[0116] Weight: (210±15)g

[0117] High-fat feed formula (by mass percentage):

[0118] Ordinary basic feed for rats 68.2%, cholesterol 1%, sodium cholate 0.8%, white sugar: 15%, egg yolk powder 5%, lard 10%.

[0119] Experimental reagents:

[0120] TC, TG, HDL-C and LDL-C detection kits (all purchased from Shanghai Fusheng Industrial Co., Ltd.)

[0121] Simvastatin (purchased from Beijing Wansheng Pharmaceutical Co., Ltd.)

[0122] (2) Experimental methods

[0123] Feeding conditions:

[0124] The rats were fed a basal diet in the experimental environment, maintained a normal day and night cycle, and had free access to water for 1 week of adaptive feeding.

[0125] Control the room temperature at 25℃ and the relative humidity at 50% to 70%.

[0126] Grouping and modeling:

[0127] After one week of adaptive feeding, 110 rats were randomly divided into two groups:

[0128] Hyperlipidemia group: 100 rats, fed with high-fat diet

[0129] Normal blood lipid group: 10 rats, fed with normal basic feed

[0130] The two groups were given free access to food and water for 3 weeks.

[0131] Blood collection and biochemical testing:

[0132] The model was established for 3 weeks. After fasting for 12 hours, blood was collected from the tail vein to collect serum samples.

[0133] TC, TG, HDL-C and LDL-C kits were used to test the levels of various blood lipids in serum.

[0134] The four blood lipid levels of rats fed with high-fat diet were significantly higher than those of rats in the normal group, indicating that the model was successful and was used in the experiment.

[0135] Experimental groups:

[0136] 100 hyperlipidemia rats with significantly higher blood lipid levels than the normal group were randomly divided into 10 groups, with 10 rats in each group, namely:

[0137] High-fat control group, positive control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group.

[0138] Dosage:

[0139] The positive control group was gavaged with 5 mg / kg simvastatin

[0140] The Example 1-4 group and the Comparative Example 1-4 group were gavaged with 0.5 mg / kg of the corresponding prepared composition

[0141] The high-fat control group and the normal blood lipid group were gavaged with the same volume of distilled water.

[0142] Oral gavage once a day for 30 days

[0143] feed:

[0144] All groups were fed a basal diet.

[0145] (3) Experimental results

[0146] During the experiment, animals were weighed daily, and their appetite, behavior, feces, hair, and mortality were observed. After 14 days of gavage, they were fasted for 12 hours with free access to water. Blood was collected from the femoral artery, allowed to rest for 30 minutes, and centrifuged at 3000 rpm for 20 minutes to separate the serum. Serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured. The results are shown in Table 1. Data were statistically analyzed and expressed as mean ± standard deviation (x ± s). Differences were analyzed using a t-test. P values ​​were calculated by comparing the high-fat model group with the blank group and each drug-treated group with the high-fat model group (P < 0.01 indicates an extremely significant difference, and P < 0.05 indicates a significant difference).

[0147] Table 1 Statistics of lipid-lowering experiment results

[0148] Note: Compared with the high-fat model group, * indicates P < 0.05, ** indicates P < 0.01

[0149] As shown in Table 1, compared to the positive control group using simvastatin, the effects of the compositions provided by the present invention in reducing total cholesterol and triglycerides in Examples 1 to 4 were similar to those in the positive control group, while the effects of Comparative Examples 1 to 4 in reducing total cholesterol and triglycerides were significantly different from those in the positive control group. Compared to the high-fat control group, the compositions provided by Examples 1 to 4 of the present invention significantly reduced total cholesterol and triglycerides, and in terms of reducing total cholesterol, the effect of any one of the Example groups was better than the sum of the effects of any two comparative examples. This indicates that the ingredients of the emblica fruit extract, mixed extract, Houttuynia cordata stem extract, and modified dietary fiber act synergistically, mutually promoting the lipid-lowering effect of the composition. Compared to the normal blood lipid group, after continuous gavage for 14 days with the compositions of Examples 1 to 4, the symptoms of hyperlipidemia in the groups were significantly reduced, approaching the blood lipid level before modeling. Mice continuously gavaged with the compositions prepared in Examples 1 to 4 had a 14-day mortality rate, indicating that the compositions prepared by the present invention are safe and non-toxic.

[0150] In summary, the composition containing the emblica fruit extract provided by the present invention can inhibit the synthesis of cholesterol and promote the metabolism of cholesterol, thereby reducing the total cholesterol level (TC) in the blood. At the same time, it can significantly reduce the concentrations of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly increase the concentration of high-density lipoprotein cholesterol (HDL-C), thereby reducing blood lipids and treating hyperlipidemia. Moreover, the main ingredients of the composition provided by the present invention, namely the emblica fruit extract, the tangerine peel extract, and the houttuynia cordata extract, are all derived from substances that are both medicinal and edible, and have little irritation to the gastrointestinal mucosa. Even if consumed for a long time, no significant side effects will be produced on the consumer.

[0151] The above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Those skilled in the art will readily appreciate that the present invention is susceptible to various modifications and variations. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of the present invention.

Claims

1. A composition of an extract of Phyllanthus emblica having a lipid-lowering function, characterized in that: The composition is composed of the following components in parts by weight: 20-30 parts of emblica fruit extract, 10-15 parts of mixed extract, 5-10 parts of houttuynia cordata stem extract, 10-20 parts of modified dietary fiber, 2-5 parts of hawthorn, 4-8 parts of kudzu root powder, and 0.1-0.3 parts of beta-carotene; The preparation method of the modified dietary fiber comprises the following steps: The peel and core of the roxburghii are peeled, washed, dried, crushed, and sieved to obtain roxburghii flesh powder, deionized water is added, the mixture is heated to 60-80° C., stirred, centrifuged, and the supernatant is retained. The residue is washed with deionized water, vacuum-dried, crushed, and sieved again. Deionized water and cellulase are then added, and the mixture is heated in a water bath to 40-50° C. for enzymolysis. After the enzymolysis is complete, the enzyme is inactivated and filtered to obtain a supernatant. The supernatants are combined and concentrated, and then a guar gum solution is added, and a homogenized shearing process is performed, followed by freeze-drying to obtain the modified dietary fiber. The preparation method of the emblica extract composition having the function of lowering blood lipids comprises the following steps: S1. Wash and remove the core of fresh emblica fruit, then crush the cored pulp and peel together with a wall-breaking machine, then microwave-treat, add an ethanol solution, stir evenly, then add glycine, heat and extract for a period of time, filter, and concentrate the filtrate to obtain the emblica fruit extract; S2, mulberry leaf, dried tangerine peel and houttuynia leaf are washed respectively, then pulverized, sieved, and mixed in proportion to obtain a mixture, and a complex enzyme consisting of cellulase and pectinase is added, and enzymolysis is carried out under ultrasonic conditions. After enzymolysis, the enzyme is inactivated, and a methanol solution is added to the mixture for extraction, filtered, and chitosan is added to the filtrate, stirred, and then methanol is recovered and concentrated under reduced pressure to obtain a mixed extract; S3, washing and crushing the stems of Houttuynia cordata, sieving, adding deionized water, stirring, then adding ethanol solution and citric acid solution, extracting under heating conditions, filtering, and evaporating and concentrating to obtain a stem extract of Houttuynia cordata; S4. Wash and remove the core of the hawthorn, crush it, and sieve it to obtain hawthorn powder. Then, mix the hawthorn powder, kudzu root powder, the emblica fruit extract, the mixed extract, the houttuynia cordata stem extract, and the modified dietary fiber in proportion, and add β-carotene in a corresponding proportion to obtain the emblica extract composition with lipid-lowering function.

2. The composition according to claim 1, wherein The mixed extract comprises a mixed extract of mulberry leaves, tangerine peel and houttuynia leaves, wherein the mass ratio of the mulberry leaves, tangerine peel and houttuynia leaves is (5-10): (2-4): (3-6).

3. The composition according to claim 1, wherein The sieved filter is 50-100 mesh, and the second sieved filter is 200-300 mesh; The mass fraction of the cellulase is 0.2-0.4%; The amount of the cellulase is 0.1-0.4% of the mass of the roxburghii; The amount of the guar gum solution is 1-2% of the mass of the roxburghii; The speed of the homogenizing shearing process is 8000-10000 rpm; The homogenization shearing treatment time is 4 to 6 minutes; The freeze-drying temperature is -30 to -20°C.

4. The composition according to claim 1, wherein In step S1, the power of the microwave treatment is 550-650 W, and the time of the microwave treatment is 3-5 min; The temperature of the heating extraction is 50-60° C., and the extraction time is 5-10 minutes.

5. The composition according to claim 1, wherein In step S1, the mass ratio of glycine to emblica fruit is (1-3): (12-15).

6. The composition according to claim 1, wherein In step S2, the mass fraction of the methanol solution is 60-80%; The power of the ultrasound is 500-600W; The extraction temperature is 40-50°C.

7. The composition according to claim 1, wherein In step S3, the mass fraction of the ethanol solution is 60-80%; The mass fraction of the citric acid solution is 20-40%; The heating temperature is 50-70°C.

8. Use of the composition according to any one of claims 1 to 4 in preparing functional foods for lowering blood lipids.

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