Giant salamander protein peptide loaded zein / xanthan gum nanoparticles as well as preparation method and application thereof
By embedding giant salamander protein peptide by zein/xanthan gum nanoparticles, the problem of low stability and bioavailability of giant salamander protein peptide in the gastrointestinal tract is solved, and its functional activity is effectively exerted.
Patent Information
- Application Number
- CN202510603791.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-12
- Publication Date
- 2025-08-01
AI Technical Summary
The stability and bioavailability of giant salamander protein peptides in the gastrointestinal tract are low, resulting in the inability to effectively exert their functional activities.
Zein/xanthan gum nanoparticles were used to embed giant salamander protein peptides, and nanoparticles loaded with giant salamander protein peptides were prepared by mixing specific proportions, sonication and vacuum freeze-drying, thereby improving their stability and bioavailability in the gastrointestinal tract.
It significantly improved the gastrointestinal stability and bioavailability of giant salamander protein peptide, and enhanced its functional activity in the body.
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Figure CN120392671A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of polypeptide processing, and particularly relates to zein / xanthan gum nanoparticles loaded with giant salamander protein peptides, and a preparation method and application thereof. Background Art
[0002] The Chinese giant salamander, known as the "ginseng in water", is a rare amphibian with high protein and low fat, and is famous for its delicious meat and rich nutritional value. However, the current product processing and utilization rate of the giant salamander industry is relatively low, and the products on the market are mostly sold in basic forms such as whole fish, fish pieces, and fish skins, lacking deep processing and diversified products. This current situation limits the development potential of the giant salamander industry, and there is an urgent need to improve its market competitiveness and economic benefits through technological innovation and product diversification.
[0003] Giant salamander protein peptides are the products of the hydrolysis of giant salamander muscle by proteases. Compared with proteins, they have significant biological functional activities such as antioxidant, anti-inflammatory, and antibacterial, which to a certain extent enriches the deep processing products of giant salamanders and increases the economic added value of artificially farmed giant salamanders. Research results show that at the cellular level, giant salamander protein peptides have the potential to improve enteritis symptoms. However, in vitro digestion results show that the activity and stability of giant salamander protein peptides are significantly reduced after gastrointestinal digestion, which means that the efficiency of human absorption and utilization of giant salamander protein peptides is not high, and thus their active functions cannot be effectively exerted. In order to improve the bioavailability of giant salamander protein peptides, further research is urgently needed. Summary of the Invention
[0004] In view of this, the purpose of the present invention is to provide zein / xanthan gum nanoparticles loaded with giant salamander protein peptides, and a preparation method and application thereof.
[0005] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:
[0006] The present invention provides zein / xanthan gum nanoparticles loaded with giant salamander protein peptides, comprising raw materials in the following mass ratio:
[0007] zein: giant salamander protein peptide: xanthan gum = 1 - 3: 0.5 - 1.5: 0.5 - 1.5.
[0008] The present invention provides a preparation method of the zein / xanthan gum nanoparticles loaded with giant salamander protein peptides, comprising the following steps:
[0009] 1) Mix an ethanol solution of zein with a giant salamander protein peptide solution to obtain a mixed solution;
[0010] 2) Mix the mixed solution with a xanthan gum solution and dry to obtain zein / xanthan gum nanoparticles loaded with giant salamander protein peptides.
[0011] Preferably, the mass concentration of zein in the zein ethanol solution is 40 - 50 mg / ml, and the mass concentration of xanthan gum in the xanthan gum solution is 7% - 10%.
[0012] Preferably, the zein ethanol solution in step 1) is prepared by mixing zein with an ethanol solution, and the volume concentration of the ethanol solution is 60% - 90%.
[0013] Preferably, the mass concentration of the giant salamander protein peptide solution in step 1) is 200 - 300 mg / ml.
[0014] Preferably, the mixing in step 1) is to combine the zein ethanol solution and the giant salamander protein peptide solution, followed by ultrasonic treatment and then stirring;
[0015] The time of the ultrasonic treatment is 10 - 30 min;
[0016] The power of the ultrasonic treatment is 240 - 480 W;
[0017] The rotation speed of the stirring is 400 - 800 rpm, the time of the stirring is 40 - 80 min, and the temperature of the mixing is 25°C.
[0018] Preferably, the rotation speed of the mixing in step 2) is 400 - 800 rpm, the mixing time is 40 - 80 min, and the temperature of the mixing is 25°C.
[0019] Preferably, the drying in step 2) is vacuum freeze-drying, the temperature of the vacuum freeze-drying is -80 to -40°C, the vacuum degree is -0.03 to -0.01 MPa, and the time is 24 - 56 h.
[0020] Compared with the prior art, the present invention has the following beneficial effects:
[0021] The present invention provides a zein / xanthan gum nanoparticle loaded with giant salamander protein peptide, its preparation method and application. The present invention embeds the giant salamander protein peptide with the zein / xanthan gum nanoparticle, which more effectively improves the gastrointestinal stability and bioavailability of the giant salamander protein peptide, and provides guidance for the high-value processing and utilization of the giant salamander protein peptide. Description of the Drawings
[0022] Figure 1 Shows the particle size and PDI coefficient of the nanocomposite at different ratios;
[0023] Figure 2 Shows the effect diagram of the ultrasonic power on the encapsulation efficiency of the nanoparticles;
[0024] Figure 3Effect diagram of the influence of ultrasonic time on the embedding efficiency of nanoparticles;
[0025] Figure 4 Effect diagram of zein / XG nanoparticles loaded with giant salamander protein peptide, sulfasalazine and curcumin;
[0026] Figure 5 Effect diagram of the influence of gastrointestinal digestion time on the cumulative release rate of nanoparticles;
[0027] Figure 6 Effect diagram of the influence of gastrointestinal digestion time on the particle size (A) and zeta potential (B) of nanoparticles;
[0028] Figure 7 Peak shapes shown by zein, CGS, XG, and zein@CGS / XG near diffraction angles 2θ of 19.27° and 20.58°;
[0029] Figure 8 Characteristic peaks of zein, CGS, XG, zein / XG, and zein@CGS / XG;
[0030] Figure 9 Fluorescence images of each major organ obtained 6 h after oral administration of each preparation;
[0031] Figure 10 Colon lengths of mice after intragastric administration of zein@CGS / XG prepared in Example 1 and treatments of Comparative Examples 1-5;
[0032] Figure 11 Comparison diagrams of colon tissues of mice after intragastric administration of zein@CGS / XG prepared in Example 1 and treatments of Comparative Examples 1-5;
[0033] Figure 12 FITC-dextran contents of mice after intragastric administration of zein@CGS / XG prepared in Example 1 and treatments of Comparative Examples 1-5;
[0034] Figure 13 Serum DAO activities of mice after intragastric administration of zein@CGS / XG prepared in Example 1 and treatments of Comparative Examples 1-5. Detailed implementation manners
[0035] The present invention provides a zein / xanthan gum nanoparticle loaded with giant salamander protein peptide, comprising raw materials in the following mass ratio:
[0036] zein: giant salamander protein peptide: xanthan gum = 1 - 3: 0.5 - 1.5: 0.5 - 1.5, preferably zein: giant salamander protein peptide: xanthan gum = 1.5 - 2.5: 0.7 - 1.2: 0.7 - 1.2, and more preferably zein: giant salamander protein peptide: xanthan gum = 2: 1: 1.
[0037] The present invention provides a method for preparing the zein / xanthan gum nanoparticles loaded with giant salamander protein peptide, comprising the following steps:
[0038] 1) Mix an ethanol solution of zein with a giant salamander protein peptide solution to obtain a mixed solution;
[0039] 2) Mix the mixed solution with a xanthan gum solution and dry to obtain the zein / xanthan gum nanoparticles loaded with giant salamander protein peptide.
[0040] In the present invention, the mass concentration of zein in the ethanol solution of zein is preferably 40 - 50 mg / ml, more preferably 42 - 48 mg / ml, and even more preferably 45 mg / ml; the method for preparing the ethanol solution of zein comprises dissolving zein in an ethanol solution, and the volume concentration of ethanol in the ethanol solution is preferably 60% - 90%, more preferably 65% - 80%, and even more preferably 70%; the mass concentration of xanthan gum in the xanthan gum solution is preferably 7% - 10%, more preferably 7.5% - 9%, and even more preferably 8%.
[0041] In the present invention, the mass concentration of the giant salamander protein peptide solution in step 1) is preferably 200 - 300 mg / ml, more preferably 220 - 280 mg / ml, and even more preferably 250 mg / ml.
[0042] In the present invention, the mixing in step 1) is to combine the ethanol solution of zein with the giant salamander protein peptide solution, followed by ultrasonic treatment and then stirring;
[0043] The time of the ultrasonic treatment is preferably 10 - 30 min, more preferably 15 - 25 min, and even more preferably 20 min;
[0044] The power of the ultrasonic treatment is preferably 240 - 480 W, more preferably 300 - 400 W, and even more preferably 360 W;
[0045] The stirring is preferably magnetic stirring, the stirring speed is preferably 400 - 800 rpm, more preferably 500 - 700 rpm, and even more preferably 600 rpm, the stirring time is preferably 40 - 80 min, more preferably 50 - 70 min, and even more preferably 60 min, and the mixing temperature is preferably 25°C.
[0046] In the present invention, the rotation speed of the mixing in step 2) is preferably 400 - 800 rpm, more preferably 500 - 700 rpm, and even more preferably 600 rpm. The mixing time is preferably 40 - 80 min, more preferably 50 - 70 min, and even more preferably 60 min. The temperature of the mixing is preferably 25 °C.
[0047] In the present invention, before drying in step 2), a centrifugation operation is further included. The centrifugation speed is preferably 2000 - 4000 rpm, more preferably 2500 - 3500 rpm, and even more preferably 3000 rpm. The precipitate obtained by centrifugation is dried. The drying is preferably vacuum freeze-drying. The temperature of the vacuum freeze-drying is preferably -80 to -40 °C, more preferably -70 to -50 °C, and even more preferably -60 °C. The vacuum degree is preferably -0.03 to -0.01 MPa, more preferably -0.02 MPa. The vacuum freeze-drying time is preferably 24 - 56 h, more preferably 30 - 48 h, and even more preferably 48 h.
[0048] The technical solutions provided by the present invention will be described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0049] Material source
[0050] The giant salamander protein peptide is provided by Zhejiang Shanding Biotechnology Co., Ltd.; xanthan gum (BR grade, S30551, Shanghai Yuanye Biotechnology Co., Ltd.); zein (BR grade, S12025, Shanghai Yuanye Biotechnology Co., Ltd.).
[0051] Example 1
[0052] This example provides a method for improving the bioavailability of giant salamander peptide by zein / XG nanoparticles. The specific process is as follows:
[0053] (1) Purify zein to obtain purified α-zein. The purification process is specifically as follows: Dissolve 5 g of zein in 100 ml of 90% ethanol, centrifuge at 10000 rpm for 20 min, take the supernatant and let it stand overnight, then centrifuge again at 10000 rpm for 20 min to remove the insoluble part. Mix distilled water and the supernatant in a volume ratio of 1:1, centrifuge again (8000 rpm, 20 min), and freeze-dry the precipitate (-60 °C, 48 h) to obtain purified α-zein.
[0054] (2) Purify xanthan gum: Dissolve the purchased commercial xanthan gum in distilled water to obtain a 10% (w / v) xanthan gum solution. After centrifugation (10000 rpm, 20 min) to remove insoluble substances, take the supernatant and perform freeze-drying (-40 °C, 72 h) to obtain purified xanthan gum (XG).
[0055] (3) Dissolve 0.5 g of zein in 9 ml of 70% ethanol, add 1 ml of the giant salamander protein peptide stock solution (250 mg of giant salamander protein peptide dissolved in 1 mL of distilled water). After sonication, transfer it to 980 ml of ultrapure water, and use a magnetic stirrer water bath to perform magnetic stirring for 1 h (600 rpm). Add 10 ml of the xanthan gum solution (25 mg / ml) and continue stirring for 1 h. After centrifugation at 3000 rpm for 10 min, take the supernatant and perform freeze-drying (-60 °C, 48 h) to obtain nanoparticles loaded with giant salamander protein peptides.
[0056] During the above experimental process, the ratio of xanthan gum to zein was adjusted respectively (1:10 - 10:1), and the particle size and PDI coefficient of the nanocomposite at different ratios were measured. The results are as Figure 1 shown. When the mass ratio of zein to XG is 1:2, 5:1, and 1:1 respectively, the PDI coefficient of zein / XG is higher, and the larger the PDI value of the system, the less stable the system. When the mass ratio of zein / XG is 2:1, the PDI reaches the lowest value. It can be concluded that a stable binary nanocomposite material is formed when the mass ratio of zein / XG is 2:1. Therefore, the present invention preferably constructs nanoparticles at a ratio of zein / XG mass ratio of 2:1.
[0057] Control the ratio of zein to xanthan gum to 2:1, and set the ultrasonic power to 0, 120, 240, 360, 480, 600 W.
[0058] Control the ratio of zein to xanthan gum to 2:1, the ultrasonic power to 360 W, and set the ultrasonic time to 0, 5, 10, 20, 30 min.
[0059] Measure the influence of different ultrasonic powers and times on the encapsulation rate of giant salamander protein peptides, as Figures 2 - 3 shown. The influence of ultrasonic power on the encapsulation efficiency of nanoparticles is as Figure 2As shown, when the ultrasonic power is 0 - 120 W, the embedding efficiency increases with the increase of power; it reaches the highest at 360 W; when the power is 360 - 600 W, the embedding efficiency decreases with the increase of power. This may be because ultrasonic treatment unfolds the structure of proteins, more amide bonds bind to hydrogen bonds, and the binding between zein and giant salamander protein peptides becomes tighter, increasing the embedding efficiency. However, excessive ultrasonic treatment induces protein unfolding and aggregation, resulting in a decrease in the embedding efficiency. The effect of ultrasonic time on the nanoparticles is as Figure 3 shown. From 0 to 20 min, the embedding efficiency increases with the increase of time; it reaches the highest at 20 min; when the time is 20 - 30 min, the embedding efficiency decreases.
[0060] Therefore, in this example, the optimal ratio of zein to xanthan gum is determined to be 2:1, the ultrasonic power is 360 W, and the ultrasonic time is 20 min. Under these conditions, the reaction is carried out to prepare zein@CGS / XG nanoparticles.
[0061] The obtained nanoparticles were gavaged to colitis mice modeled with 3% DSS at a dose of 100 mg / kg of the loaded giant salamander protein peptide. The method for colitis modeling refers to the method of Chen et al. (R. Chen, X. Lin, Q. Wang, X. An, X. Zhao, Y. Lin, T. Sun, C. Yan, A. Cai, W. Cao, Y. Zhang, Q. Yao, L. Kou, Dual-targeting celecoxib nanoparticles protect intestinal epithelium and regulate macrophage polarization for ulcerative colitis treatment, Chemical Engineering Journal 452 (2023)).
[0062] Comparative Example 1
[0063] The difference between Comparative Example 1 and Example 1 is that normal mice were gavaged with sterile water.
[0064] Comparative Example 2
[0065] The difference between Comparative Example 2 and Example 1 is that colitis mice modeled with 3% DSS were gavaged with sterile water.
[0066] Comparative Example 3
[0067] The difference between Comparative Example 3 and Example 1 is that colitis mice modeled with 3% DSS were gavaged with 500 mg / kg of zein / XG blank nanoparticle carriers without loading giant salamander protein peptides.
[0068] Comparative Example 4
[0069] The difference between Comparative Example 4 and Example 1 is that 100 mg / kg sulfasalazine (SASP) was used for gavage in the 3% DSS-induced model.
[0070] Comparative Example 5
[0071] The difference between Comparative Example 5 and Example 1 is that 100 mg / kg free giant salamander protein peptide was used for gavage in the 3% DSS-induced enteritis mice.
[0072] Comparative Example 6
[0073] The difference between Comparative Example 6 and Example 1 is that SASP was used instead of giant salamander protein peptide for the preparation of nanoparticles.
[0074] Comparative Example 7
[0075] The difference between Comparative Example 7 and Example 1 is that curcumin (Cur) was used instead of giant salamander protein peptide for the preparation of nanoparticles.
[0076] Experimental Example 1
[0077] The loading rate of zein@CGS / XG nanoparticles prepared under the optimal conditions of Example 1, Comparative Example 6, and Comparative Example 7 was measured as shown below. Figure 4 The results show that zein / XG nanoparticles are more effective for encapsulating giant salamander protein peptide.
[0078] Meanwhile, in vitro digestion, XRD, Fourier transform infrared spectroscopy, and targeting analysis of Example 1 were carried out as shown in Figure 5 、 6 、7, 8, 9, 10.
[0079] As shown in Figure 5 、 6 When digested in the stomach for 2 h, the cumulative release rate of the nanoparticles reached about 20%; the release rate increased with time from 2 to 3 h and reached 80%; the release rate tended to level off from 3 to 6 h, and the cumulative release rate reached about 90% at 360 min. The hydrophilic outer shell (XG) can prevent the enzymatic degradation of protein (zein) in the gastrointestinal environment, so the nanoparticles can provide protection for giant salamander protein peptide and start to release the peptide in the intestinal fluid environment, and the release effect increases with time, thus achieving a sustained release effect. Figure 6 When digested in the stomach for 2 h, the nanoparticles aggregated in the gastric juice, and the particle size of the digestion products reached the maximum, which verified the conjecture that the nanoparticles can protect giant salamander protein peptide from being digested and destroyed in the stomach.
[0080] As shown in Figure 7As shown, zein and CGS respectively exhibit relatively flat humps near diffraction angles 2θ of 19.27° and 20.58°, with a wide and flat range; XG has a diffraction peak with a gentle peak shape at a diffraction angle 2θ of 18.87°, indicating that zein, CGS, and XG all exhibit an amorphous and non-highly crystalline structure. In addition, compared with the peak shape of zein, the intensity of the slow peak of the formed composite nanoparticles slightly decreases, which may be due to the increased interaction strength between zein and XG, causing more CGS to be encapsulated in the composite.
[0081] As Figure 8 shown, all samples showed a relatively wide band at a wavenumber of 3500 - 3200 cm -1 , which is caused by the stretching vibration of the O-H group. Among them, the characteristic peak of zein is at 3314.63 cm -1 . When zein and XG combine to form nanoparticles, it migrates to 3323.39 cm -1 , which may be due to the hydrogen bond interaction between the amide group of zein and the hydroxyl group of xanthan gum. Zein has two typical amide peaks, representing amide I (1658.16 cm -1 ) for C=O stretching and amide II (1542.41 cm -1 ) for N-H bending, while the amide peak of XG is not significant; a characteristic peak of XG at 1030 cm -1 is an absorption peak generated by the coupling vibration of C-O-C-O-C acetal, which zein does not have; after forming zein-XG binary composite nanoparticles, it has both the characteristic peaks of zein and XG, indicating that zein and XG are successfully combined. Compared with zein alone, its amide I and amide II migrate to 1650.62 cm -1 and 1531.66 cm -1 respectively, indicating that the combination of zein and XG is achieved through hydrophobic and electrostatic interactions. The infrared spectra of the composite nanoparticles loaded with polypeptides and the zein-XG composite without loaded polypeptides are generally consistent, and the characteristic peaks of polypeptides cannot be clearly observed, indicating that the polypeptides are well encapsulated in the nanoparticles.
[0082] Label the zein@CGS / XG nanoparticles prepared under the optimal conditions of Example 1 and the giant salamander protein peptide (CGS) of Comparative Example 5 with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR). The labeling method is as follows: Dissolve 100 mg of CGS in 50 ml of distilled water, add 1 mg of DiR, stir magnetically in the dark for 2 h, dialyze (MW 300) for 72 h, and then freeze-dry (-60 °C, 48 h) for subsequent use; Dissolve 0.5 g of zein in 8 ml of 70% ethanol, add 1 ml of DiR solution (0.5 mg of DiR dissolved in 1 mL of absolute ethanol) and 1 ml of giant salamander protein peptide stock solution (250 mg of giant salamander protein peptide dissolved in 1 mL of distilled water), after ultrasonic treatment, transfer it to 980 ml of ultrapure water, use a magnetic stirrer water bath to stir magnetically in the dark for 1 h (600 rpm), add 10 ml of xanthan gum solution (25 mg / ml), continue to stir for 1 h, centrifuge at 3000 rpm for 10 min, and take the supernatant to obtain zein@CGS / XG-DiR, dialyze (MW 300) for 72 h, and then freeze-dry (-60 °C, 48 h) for subsequent use. Use a UV-visible spectrophotometer to make a DiR standard curve to determine the DiR loading amount. According to the dose of DiR 0.5 mg / kg, respectively intragastrically administer Free-DiR, CGS-DiR, and zein@CGS / XG-DiR to the colitis mice modeled with 3% DSS (the method of colitis modeling refers to the method of Chen et al. [1] (The method is divided into three groups, namely the Free-DiR group, the CGS-DiR group, and the zein@CGS / XG-DiR group. After 6 h, decapitate and sacrifice the mice and collect their main organs such as the heart, liver, kidney, and gastrointestinal tract. Use a small animal in vivo imaging system to record their fluorescence images and use Aniview software to quantitatively analyze the fluorescence intensity of the colon part. As Figure 9 shown. The fluorescence signal mainly appears in the gastrointestinal tract, and no obvious fluorescence signal is seen in other organs. Interestingly, it is found by fluorescence that the gastrointestinal tract signal of the nanoparticle embedding group is significantly stronger than that of Free-DiR and CGS-DiR, especially in the colitis tissue.
[0083] Experimental Example 2
[0084] After sacrificing the mice in each group, analyze the colon length, He staining, and intestinal permeability of the mice intragastrically administered with the zein@CGS / XG nanoparticles prepared under the optimal conditions of Example 1 and Comparative Examples 1-5. The results are as Figure 10 、 11 、12, 13 shown.
[0085] As Figure 10As shown, the colon length of the mice in Comparative Example 2 was significantly lower than that in Comparative Example 1, and the colon lengths of the mice in Comparative Example 4 and Example 1 were significantly increased, indicating that the nanoparticles encapsulating the polypeptide can significantly alleviate the signs of disease in DSS-induced mice.
[0086] As Figure 11 shown, the colon tissue of Comparative Example 1 showed a normal morphology, with a complete mucosa, abundant goblet cells, and no swelling in the muscular layer and submucosa. Compared with Comparative Example 1, the colon of the mice in Comparative Example 2 was severely damaged, showing obvious inflammatory symptoms, including severe damage to epithelial cells, loss of colon cells, goblet cell exhaustion, crypt distortion, muscular layer edema and thickening, mucosal inflammation, and extensive inflammatory cell infiltration, and there was no significant difference between Comparative Example 3 and it, indicating that the blank nanoparticle carrier does not have the function of alleviating enteritis. Pretreatment with SASP and Andrias davidianus protein peptide nanoparticles attenuated the pathological changes induced by DSS, and both maintained the morphological integrity of the colon tissue. The inflammatory signs in Comparative Examples 5 and 6 were significantly reduced. Among them, compared with Comparative Example 5, the colon morphology of the mice in Comparative Example 6 was more complete, and its state was closest to that in Comparative Example 4. This shows that Andrias davidianus protein peptide can alleviate the colon tissue damage induced by DSS, and it is more effective after encapsulation with nanoparticles.
[0087] As Figure 12 shown, compared with Comparative Example 1, DSS administration significantly increased the FITC-dextran content in the plasma of mice, while the FITC-dextran content in the mice of Comparative Example 4, Comparative Example 5, and Example 1 was improved to varying degrees and was significantly lower than that in Comparative Example 2. The results indicate that Andrias davidianus protein peptide significantly inhibits the increase in intestinal epithelial permeability caused by DSS.
[0088] As Figure 13 shown, compared with Comparative Example 1, the serum DAO activity of the mice in Comparative Example 2 was significantly increased, while the serum DAO activities of the mice in Comparative Example 4, Comparative Example 5, and Example 1 were significantly decreased. These results confirm that Andrias davidianus protein peptide can improve the increase in intestinal permeability induced by DSS, and it is more effective after encapsulation with nanoparticles.
[0089] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.
Claims
1. A zein / xanthan gum nanoparticle loaded with giant salamander protein peptide, characterized in that, It comprises raw materials with the following mass ratios: Zein: Andrias davidianus protein peptide: Xanthan gum = 1 - 3: 0.5 - 1.5: 0.5 - 1.
5.
2. The preparation method of the zein / xanthan gum nanoparticles loaded with giant salamander protein peptides according to claim 1, characterized in that, It comprises the following steps: 1) Mix the ethanol solution of zein with the Andrias davidianus protein peptide solution to obtain a mixed solution; 2) Mix the mixed solution with the xanthan gum solution and dry it to obtain zein / xanthan gum nanoparticles loaded with Andrias davidianus protein peptide.
3. The preparation method according to claim 2, wherein The mass concentration of zein in the ethanol solution of zein is 40 - 50 mg / ml, and the mass concentration of xanthan gum in the xanthan gum solution is 7% - 10%.
4. The method according to claim 3, characterized in that The ethanol solution of zein in step 1) is prepared by mixing zein with an ethanol solution, and the volume concentration of the ethanol solution is 60% - 90%.
5. The method according to claim 4, wherein The mass concentration of the Andrias davidianus protein peptide solution in step 1) is 200 - 300 mg / ml.
6. The method according to claim 5, characterized in that, The mixing in step 1) is to combine the ethanol solution of zein with the Andrias davidianus protein peptide solution, followed by ultrasonic treatment and then stirring; The time of the ultrasonic treatment is 10 - 30 min; The power of the ultrasonic treatment is 240 - 480 W; The rotation speed of the stirring is 400 - 800 rpm, the time of the stirring is 40 - 80 min, and the temperature of the mixing is 25°C.
7. The preparation method according to claim 2, characterized in that, The rotation speed of the mixing in step 2) is 400 - 800 rpm, the mixing time is 40 - 80 min, and the temperature of the mixing is 25°C.
8. The preparation method according to claim 7, wherein The drying in step 2) is vacuum freeze-drying, the temperature of the vacuum freeze-drying is -80 - -40°C, the vacuum degree is -0.03 - -0.01 MPa, and the time is 24 - 56 h.