Microfluidic detection chip, matched detection equipment and detection method
Through the microfluidic detection chip and supporting equipment, the magnetic parts move outside the reagent cavity to achieve transfer and mixing of magnetic particles, solving the problems of complex and susceptible to contamination in the prior art, and achieving the effect of simplifying operation and reducing contamination.
Patent Information
- Application Number
- CN202510626695.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-15
- Publication Date
- 2025-08-01
AI Technical Summary
In the prior art, the nucleic acid extraction process is complex, requires a professional environment and is susceptible to contamination.
Using a microfluidic detection chip and supporting detection equipment, the first and second magnetic parts are used to move longitudinally and laterally outside the reagent cavity to realize drag, mix and resuspend the magnetic particles. The magnetic particles are in a closed environment when they are transferred between each reagent cavity.
The nucleic acid extraction steps are simplified, the operation complexity and contamination probability are reduced, and the detection accuracy is improved.
Smart Images
Figure CN120400312A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of microfluidic chips, and particularly to a microfluidic detection chip, a supporting detection device and a detection method. Background Art
[0002] In the related art, during the process of nucleic acid extraction using the magnetic bead method or the silica gel membrane method (centrifugal column method), complex mechanical disturbance structures are required for operation, and the operation is complex; moreover, during the operation process, high requirements are imposed on the operation environment, and a professional molecular diagnosis laboratory is needed to reduce the probability of aerosol contamination. Summary of the Invention
[0003] The present application aims to solve at least one of the technical problems existing in the prior art. For this purpose, the present application provides a microfluidic detection chip, a supporting detection device and a detection method, which can simplify the nucleic acid extraction steps and reduce the probability of being contaminated.
[0004] An embodiment of the first aspect of the present application provides a detection method for a microfluidic detection chip, which is applied to a microfluidic detection chip. The microfluidic detection chip includes a substrate and a top sealing layer disposed on the upper side of the substrate. A plurality of reagent chambers are spaced apart on the substrate, and a drag channel is disposed between two adjacent reagent chambers. The drag channel abuts against the top sealing layer. The method includes:
[0005] S110: Set a first magnetic member above the top sealing layer and set a second magnetic member below the substrate;
[0006] S120: Drive the first magnetic member close to the top sealing layer to gather the magnetic particles in the reagent chamber on the side of the top sealing layer located in the reagent chamber;
[0007] S130: Drive the first magnetic member to move away from the top sealing layer, and at the same time drive the second magnetic member to move close to the bottom of the substrate to adsorb the magnetic particles to the bottom of the reagent chamber;
[0008] S140: Drive the second magnetic member away from the substrate and drive the first magnetic member close to the top sealing layer to adsorb the magnetic particles to the side of the top sealing layer located in the reagent chamber;
[0009] S150: Drive the first magnetic member and the second magnetic member to move laterally so that the magnetic particles pass through the drag channel and are transferred to the next reagent chamber.
[0010] It can be seen from the above technical solutions that the embodiments of the present application have at least the following beneficial effects:
[0011] In the detection method of the microfluidic detection chip provided by the embodiment of the present application, processes such as dragging, mixing, and resuspending the magnetic particles in the reagent chamber are realized by the longitudinal and lateral movements of the external first magnetic member and second magnetic member outside the reagent chamber, without the need for complex mechanical perturbation structures, which is conducive to simplifying the operation steps. At the same time, during the transfer of the magnetic particles between the reagent chambers, they are always in a closed environment, which is conducive to reducing the probability of the reagent in the reagent chamber being contaminated.
[0012] Further, after step S150, drive the second magnetic member close to the bottom of the substrate, and drive the first magnetic member away from the top sealing layer to adsorb the magnetic particles to the bottom of the reagent chamber.
[0013] Further, after step S140, it further includes: controlling the lateral movement of the first magnetic member to move the magnetic particles to multiple positions within the reagent chamber.
[0014] Further, after step S140, it includes: repeating steps S120, S130, and S140 multiple times in sequence.
[0015] Further, the substrate is provided with a gas docking hole and a sample chamber. The sample chamber is in fluid communication with the first reagent chamber, and the gas docking hole is in fluid communication with the second reagent chamber. When it is necessary to drive the reagent in the sample chamber to transfer to the first reagent chamber, air is extracted through the gas docking hole to make the reagent chamber in a negative pressure state.
[0016] Further, the substrate is provided with a reaction chamber. The reaction chamber is in fluid communication with the last reagent chamber. When it is necessary to transfer the elution product in the last reagent chamber to the reaction chamber, air is injected into the second reagent chamber through the gas docking hole.
[0017] Further, when the elution product is transferred from the reagent chamber to the reaction chamber, control the first magnetic member to approach the top sealing layer to keep the first magnetic member adsorbing the magnetic particles.
[0018] An embodiment of the second aspect of the present application provides a microfluidic detection chip, which adopts the detection method as described above to transfer magnetic particles between each cavity.
[0019] An embodiment of the third aspect of the present application provides a supporting detection device, including:
[0020] A magnetic component, including a first magnetic member and a second magnetic member arranged at intervals;
[0021] A driving device is used to drive the first magnetic member to move, so that the first magnetic member moves in a direction perpendicular to the microfluidic detection chip, and the driving device is also used to drive the first magnetic member to move laterally along the end face parallel to the microfluidic detection chip; the driving device is used to drive the second magnetic member to move, so that the second magnetic member moves in a direction perpendicular to the microfluidic detection chip, and the driving device is also used to drive the second magnetic member to move laterally along the end face parallel to the microfluidic detection chip.
[0022] Furthermore, the supporting detection device includes a gas driving control structure. The gas driving control mechanism is used to suck or blow air into the gas docking hole, and the gas driving control structure is also used to control the on-off of the gas docking hole. Description of the Drawings
[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.
[0024] Figure 1 It is a schematic flowchart of the detection method of the microfluidic detection chip provided by an embodiment of the present application;
[0025] Figure 2 It is a schematic diagram of the actions of the first magnetic member and the second magnetic member in one of the steps of an embodiment of the present application;
[0026] Figure 3 It is a schematic diagram of the actions of the first magnetic member and the second magnetic member in one of the steps of an embodiment of the present application;
[0027] Figure 4 It is a schematic diagram of the actions of the first magnetic member and the second magnetic member in one of the steps of an embodiment of the present application.
[0028] Reference Signs:
[0029] 1. Magnetic particles; 2. First magnetic member; 3. Second magnetic member;
[0030] 110. Substrate; 111. Reagent chamber; 112. Sample chamber; 120. Top sealing layer; 130. Bottom sealing layer. Detailed Embodiments
[0031] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present application without creative efforts shall fall within the protection scope of the present application.
[0032] See Figure 1 and Figure 2 As shown, an embodiment of the first aspect of the present application discloses a detection method for a microfluidic detection chip, which is applied to a microfluidic detection chip. The microfluidic detection chip includes a substrate 110 and a top sealing layer 120 provided on the upper side of the substrate 110. A plurality of reagent chambers 111 are provided on the substrate 110 at intervals, and a drag channel is provided between two adjacent reagent chambers 111. The drag channel abuts against the top sealing layer 120. Among them, the method includes:
[0033] S110: Place the first magnetic member 2 above the top sealing layer 120, and place the second magnetic member 3 below the substrate 110;
[0034] S120: Drive the first magnetic member 2 close to the top sealing layer 120 to gather the magnetic particles 1 in the reagent chamber 111 on one side of the top sealing layer 120 located in the reagent chamber 111;
[0035] S130: Drive the first magnetic member 2 to move away from the top sealing layer 120, and at the same time drive the second magnetic member 3 to move close to the bottom of the substrate 110 to adsorb the magnetic particles 1 to the bottom of the reagent chamber 111;
[0036] S140: Drive the second magnetic member 3 away from the substrate 110, and drive the first magnetic member 2 close to the top sealing layer 120 to adsorb the magnetic particles 1 to one side of the top sealing layer 120 located in the reagent chamber 111;
[0037] S150: Drive the first magnetic member 2 and the second magnetic member 3 to move laterally to transfer the magnetic particles 1 through the drag channel to the next reagent chamber 111.
[0038] In the detection method of the microfluidic detection chip provided by the embodiments of the present application, the processes of dragging, mixing, and resuspending the magnetic particles 1 located in the reagent chamber 111 are realized by the longitudinal and lateral movements of the external first magnetic member 2 and second magnetic member 3 outside the reagent chamber 111, without complex mechanical disturbance structures, which is beneficial to simplifying the operation steps. At the same time, during the transfer of the magnetic particles 1 between the reagent chambers 111, they are always in a closed environment, which is beneficial to reducing the probability of being contaminated.
[0039] In this embodiment, the microfluidic detection chip includes a substrate 110, a top sealing layer 120, and a bottom sealing layer 130. The top sealing layer 120 is disposed on the top of the substrate 110, and the bottom sealing layer 130 is disposed on the bottom of the substrate 110.
[0040] In some embodiments of the present application, referring to Figure 3 and Figure 4 , after the magnetic particles 1 move from the previous reagent chamber 111 to the next reagent chamber 111, the second magnetic member 3 is driven to approach the bottom sealing layer 130, and the first magnetic member 2 is driven away from the top sealing layer 120 to adsorb the magnetic particles 1 to the bottom of the reagent chamber 111. During this process, the magnetic particles 1 can be fully mixed with the reagent in the reagent chamber 111.
[0041] In some embodiments of the present application, after step S140, the detection method includes: controlling the first magnetic member 2 to move laterally so that the magnetic particles 1 move to multiple positions in the reagent chamber 111. In this way, it is beneficial to fully mix the magnetic particles 1 with the reagent in the reagent chamber 111, thereby improving the cleaning or elution effect.
[0042] In some embodiments of the present application, after step S140, that is, after the magnetic particles 1 are transferred to the bottom of the reagent chamber 111, steps S120, S130, and S140 are repeated multiple times. Thereby, the magnetic particles 1 can be fully contacted and mixed with the reagent in the reagent chamber 111.
[0043] In some embodiments of the present application, the substrate 110 is provided with a gas docking hole and a sample chamber 112. The sample chamber 112 is in fluid communication with the first reagent chamber 111, and the gas docking hole is in fluid communication with the second reagent chamber 111. When it is necessary to drive the reagent in the sample chamber 112 to transfer to the first reagent chamber 111, air is extracted through the gas docking hole. In this way, a negative pressure environment can be created in the reagent chamber 111 to facilitate the smooth transfer of the reagent solution in the sample chamber 112 to the next reagent chamber 111 under the action of negative pressure, which is beneficial to simplifying the operation steps of transferring the reagent solution. During this process, the reagent solution is in a closed environment, and the probability of being contaminated can be reduced.
[0044] It should be understood that when air is extracted from the gas docking hole, both the reagent chamber 111 and the sample chamber 112 are in a sealed environment.
[0045] In some embodiments of the present application, the substrate 110 is provided with a reaction chamber, and the reaction chamber is in fluid communication with the last reagent chamber 111. When it is necessary to transfer the elution product in the last reagent chamber 111 to the reaction chamber, air is injected into the second reagent chamber 111 through the gas docking hole. In this way, it is convenient to transfer the elution product in the reagent chamber 111 to the reaction chamber.
[0046] It should be noted that the liquid outlet end of the reaction chamber is connected to a hydrophobic breathable membrane chamber or a gas pore. When transferring the elution product into the reaction chamber, the gas in the reaction chamber can be discharged to the outside of the microfluidic detection chip through the hydrophobic breathable membrane chamber or the gas pore, while the liquid reagent cannot be discharged from the hydrophobic breathable membrane chamber.
[0047] Among them, a cut-off valve is provided between the reagent chamber 111 at the end and the reaction chamber to control the on-off of the flow channel between the reagent chamber 111 at the end and the reaction chamber; similarly, another cut-off valve is provided in the flow channel between the liquid outlet end of the reaction chamber and the hydrophobic breathable membrane chamber or the gas pore to control the on-off of the flow channel between the liquid outlet end of the reaction chamber and the hydrophobic breathable membrane chamber or the gas pore.
[0048] Furthermore, when transferring the elution product from the reagent chamber 111 to the reaction chamber, the first magnetic member is controlled to approach the top sealing layer 120 so that the first magnetic member 2 maintains the adsorption effect on the magnetic particles 1, thereby avoiding the situation where the magnetic particles 1 enter the reaction chamber.
[0049] An embodiment of the second aspect of the present application discloses a microfluidic detection chip, which adopts the foregoing detection method to transfer magnetic particles between each cavity.
[0050] Among them, the microfluidic detection chip includes a substrate 110 and a top sealing layer 120 and a bottom sealing layer 130 provided on both sides of the substrate 110. The substrate 110 is provided with a plurality of reagent chambers 111 arranged at intervals, and a drag channel is provided between two adjacent reagent chambers 111. The drag channel abuts against the top sealing layer 120. When the magnetic particles 1 are transferred between each reagent chamber 111, the magnetic particles 1 are always in a closed environment, which is beneficial to reducing the probability of being contaminated, thereby ensuring the accuracy of the detection result.
[0051] In one embodiment, at least one buffer chamber is provided between two adjacent reagent chambers 111. The buffer chamber is used to accommodate the reagent overflowing from the adjacent reagent chamber 111 to prevent the reagents in the two adjacent reagent chambers 111 from mixing with each other, and reduce the contamination caused by the mutual mixing of different reagents.
[0052] An embodiment of the third aspect of the present application discloses a supporting detection device, including:
[0053] A magnetic assembly, including a first magnetic member 2 and a second magnetic member 3 arranged at intervals;
[0054] The driving device is used to drive the first magnetic member 2 to move, so that the first magnetic member 2 moves in a direction perpendicular to the microfluidic detection chip, and the driving device is also used to drive the first magnetic member 2 to move laterally along the end face parallel to the microfluidic detection chip; the driving device is used to drive the second magnetic member 3 to move, so that the second magnetic member 3 moves in a direction perpendicular to the microfluidic detection chip, and the driving device is also used to drive the second magnetic member 3 to move laterally along the end face parallel to the microfluidic detection chip.
[0055] In some embodiments of the present application, a driving device drives the first magnetic member 2 and the second magnetic member 3 to move synchronously, so as to drag the magnetic particles 1 to move between different positions in the reagent chamber 111, or drag the magnetic particles 1 to transfer between different reagent chambers 111.
[0056] In a possible implementation manner, the driving device includes a U-shaped bracket. The first magnetic member 2 is connected to one end of the U-shaped bracket, and the second magnetic member 3 is connected to the other end of the U-shaped bracket. Among them, the microfluidic detection chip is located between the first magnetic member 2 and the second magnetic member 3. By driving the U-shaped bracket to move laterally, the driving device can drive the first magnetic member 2 and the second magnetic member 3 to move synchronously laterally. By driving the U-shaped bracket to move along the connection line direction between the first magnetic member 2 and the second magnetic member 3, the first magnetic member 2 can be made to move away from the microfluidic detection chip while the second magnetic member 3 moves closer to the microfluidic detection chip, or the first magnetic member 2 can be made to move closer to the microfluidic detection chip while the second magnetic member 3 moves away from the microfluidic detection chip. Among them, the connection line direction between the first magnetic member 2 and the second magnetic member 3 is perpendicular to the microfluidic detection chip.
[0057] In some other embodiments, there may be two driving devices, namely a first driving device and a second driving device. The first driving device is used to drive the first magnetic member 2 to move independently, and the second driving device is used to drive the second magnetic member 3 to move independently. For example, the first driving device is used to drive the first magnetic member 2 to move, so that the first magnetic member 2 moves in a direction perpendicular to the microfluidic detection chip, and the driving device is also used to drive the first magnetic member 2 to move laterally along the end face parallel to the microfluidic detection chip, so as to drive the magnetic particles 1 to move laterally.
[0058] The supporting detection device of this embodiment is used to execute the foregoing control method to realize the detection of the microfluidic detection chip. It is worth mentioning that the action control of the supporting detection device can refer to the above definition of the control method, and will not be elaborated here.
[0059] In one embodiment, the supporting detection device includes a gas driving control mechanism. The gas driving control mechanism is used to suck air or blow air into the gas docking hole, and the gas driving control structure is also used to control the on-off of the gas docking hole, so that the gas docking hole can communicate with the external atmospheric environment, thereby balancing the air pressure inside and outside the chip.
[0060] The detection method, microfluidic detection chip and supporting detection equipment of the microfluidic detection chip according to the embodiments of the present application will be described in detail below with a specific embodiment. It should be noted that the following embodiments are only exemplary descriptions and should not be construed as limitations on the embodiments of the present application.
[0061] See Figures 1 to 4 As shown, in this embodiment, the microfluidic detection chip includes a sample chamber 112, a reagent chamber 111 and a reaction chamber. There are five reagent chambers 111. Along the flow direction of the fluid, each reagent chamber 111 is successively a first reagent chamber, a second reagent chamber, a third reagent chamber, a fourth reagent chamber and a fifth reagent chamber. Among them, the sample chamber 112 is in fluid communication with the first reagent chamber through a flow channel; the fifth reagent chamber is in fluid communication with the reaction chamber through another flow channel; a buffer chamber is provided between two adjacent reagent chambers, and a drag channel is provided between the buffer chamber and the reagent chamber.
[0062] In this embodiment, the sample chamber 112 is used to hold the lysis binding solution, the second reagent chamber is used to hold the cleaning solution a, the third reagent chamber is used to hold the cleaning solution b, the fourth reagent chamber is used to hold the cleaning solution c, the fifth reagent chamber is used to hold the eluent, and the reaction chamber is used to hold the amplification reagent (such as PCR reagent, isothermal amplification reagent, etc.).
[0063] The detection method of this embodiment specifically includes the following steps:
[0064] Step 1: Add a certain volume of biological sample (including but not limited to blood sample, respiratory sample, urine sample, fecal sample, cell culture, etc.) to the sample chamber 112 of the microfluidic detection chip, close the chamber lid of the sample chamber 112, and insert the whole microfluidic detection chip into the supporting detection equipment, and the supporting detection equipment starts to run according to the set program;
[0065] Step 2: Add a lysis solution into the sample chamber 112 to form a sample mixture of the lysis solution and the biological sample in the sample chamber 112, and add substances such as magnetic particles 1 and digestive enzymes into the sample chamber 112;
[0066] Step 3: At this time, the gas drive control structure inside the supporting detection equipment is docked with the gas docking hole on the detection chip, and at the same time, the first cut-off valve arranged on the flow channel between the fifth reagent chamber and the reaction chamber is controlled by the valve control structure inside the supporting detection equipment to make the first cut-off valve in a closed state. At this time, the flow channel between the liquid inlet end of the reaction chamber and the fifth reagent chamber is blocked by the first cut-off valve;
[0067] Step 4: The gas control structure inside the supporting detection device extracts a certain amount of gas from the gas docking hole, making each reagent chamber in a negative pressure state. Due to the pressure difference, substances such as the sample mixture, magnetic particles 1, and digestive enzymes inside the sample chamber 112 can all be transferred to the first reagent chamber. At this time, some spaces inside the chip, such as the liquid reagent chamber, will be in a negative pressure state;
[0068] Step 5: Maintain the negative pressure state, and let the mixture composed of biological samples, lysis binding solution, magnetic particles 1, and digestive enzymes stand and incubate in the first reagent chamber for a certain period of time. During the incubation process, thermal incubation can be selected with the help of equipment;
[0069] Step 6: The supporting detection device sets a first magnetic part 2 above the top sealing layer 120 above the first reagent chamber and a second magnetic part 3 below the bottom sealing layer 130 below the first reagent chamber. Among them, the first magnetic part 2 and the second magnetic part 3 can use electromagnets or permanent magnets. In this embodiment, permanent magnets are preferably used, and the supporting detection device controls the actions of the first magnetic part 2 and the second magnetic part 3;
[0070] Step 7: Drive the first magnetic part 2 to move downward close to the top sealing layer 120 at the first reagent chamber to adsorb the magnetic particles 1 in the first reagent chamber until the magnetic particles 1 are adsorbed and aggregated on one side of the top sealing layer 120 inside the first reagent chamber;
[0071] Step 8: At this time, drive the first magnetic part 2 to move upward away from the top sealing layer 120 of the first reagent chamber, and drive the second magnetic part 3 to move upward close to the bottom sealing layer 130 of the first reagent chamber, and adsorb the magnetic particles 1 from the top sealing layer 120 downward to the bottom of the first reagent chamber;
[0072] Step 9: Steps 7 and 8 can be repeated several times;
[0073] Step 10: The second magnetic part 3 moves away from the bottom of the first reagent chamber, and the first magnetic part 2 moves close to the top of the first reagent chamber to adsorb the magnetic particles 1 in the first reagent chamber to one side of the top sealing layer 120 inside the first reagent chamber;
[0074] Step 11: The gas control structure inside the supporting detection device that is docked with the gas docking hole relieves the negative pressure state inside the chip, making the gas pressure inside the microfluidic detection chip the same as the external environmental air pressure and maintaining the air pressure balance state;
[0075] Step 12: The supporting detection device sequentially adds eluent, cleaning solution c, cleaning solution b, and cleaning solution a to the fifth reagent chamber, the fourth reagent chamber, the third reagent chamber, and the second reagent chamber through the corresponding flow channels;
[0076] Step 13: Horizontally move the first magnetic member 2 and synchronously move the second magnetic member 3 to transfer the magnetic particles 1 through the dragging channel to the buffer chamber between the first reagent chamber and the second reagent chamber, and continue to drag them through the pushing and dragging channel to the side of the top sealing layer 120 above the second reagent chamber that is located inside the second reagent chamber;
[0077] Step 14: Move the second magnetic member 3 upward close to the bottom sealing layer 130 below the second reagent chamber, while the first magnetic member 2 moves upward away from the top sealing layer 120, so that the magnetic particles 1 are adsorbed to the bottom of the second reagent chamber;
[0078] Step 15: At this time, the first magnetic member 2 moves downward close to the top sealing layer 120 of the second reagent chamber, and at the same time the second magnetic member 3 moves away from the bottom sealing layer 130 below the second reagent chamber, and the magnetic particles 1 are adsorbed to the side of the top sealing layer 120 above the second reagent chamber that is located inside the second reagent chamber again;
[0079] Step 16: Repeat steps 14 and 15 several times; of course, the first magnetic member 2 can also be horizontally moved to other multiple positions of the top sealing layer 120 above the second reagent chamber, and steps 14 and 15 are repeated several times to fully wash the magnetic particles 1 in different regions of the washing liquid b in the second reagent chamber to remove impurities;
[0080] Step 17: According to the above method, drag the magnetic particles 1 to the third reagent chamber (with washing liquid b injected inside) and the fourth reagent chamber (with washing liquid c injected inside) in sequence for washing.
[0081] Step 18: Finally, drag the magnetic particles 1 to the fifth reagent chamber, and then according to the method of the up-and-down and horizontal movement of the first magnetic member 2 and the second magnetic member 3 described in steps 14, 15, and 16, release the nucleic acid adsorbed on the surface of the magnetic particles 1 into the eluent, so as to obtain a nucleic acid elution product.
[0082] During the elution process, an auxiliary device can also be selected for thermal incubation during the elution process.
[0083] Step 19: Keep the adsorption state of the first magnetic member 2 on the magnetic particles 1 in the fifth reagent chamber, open the first stop valve, and close the third stop valve arranged at the liquid outlet end of the reaction chamber, where the third stop valve is arranged on the flow channel between the liquid outlet end of the reaction chamber and the test strip accommodation chamber;
[0084] Step 20: The gas driving control structure in the supporting detection device docks with the gas docking hole and injects a certain amount of air into the chip to push the liquid nucleic acid elution product in the fifth reagent chamber into the reaction chamber, where a dry amplification reagent is preset in the reaction chamber;
[0085] Step 21: Close the first stop valve and the second stop valve. The second stop valve is arranged between the liquid outlet end of the reaction chamber and the hydrophobic breathable membrane chamber. With the support of the supporting detection equipment, an amplification reaction is carried out inside the reaction chamber;
[0086] Step 22: After the amplification reaction ends, open the first stop valve and the third stop valve. The gas driving control structure inside the supporting detection equipment docks with the gas docking hole and injects a certain amount of air into the microfluidic detection chip, so that the amplification product in the reaction chamber enters the test strip accommodating chamber through the flow channel and the third stop valve, so that the amplification product contacts the chromatographic membrane strip in the reagent accommodating chamber for chromatographic detection;
[0087] Step 23: During the chromatographic detection process, the third stop valve can be selected to be closed, or the positive pressure state inside each flow channel of the detection chip connected to the gas docking hole can be maintained;
[0088] Step 24: After the chromatographic detection ends, the internal structure of the supporting detection equipment is reset, and the microfluidic detection chip is taken out.
[0089] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.
[0090] The terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, unless otherwise specified, the meaning of "a plurality" is two or more.
[0091] In the description of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific situations.
[0092] In the description of this specification, specific features, structures, materials, or characteristics can be combined in a suitable manner in any one or more embodiments or examples.
[0093] It should be understood that although the steps in the flowchart of the accompanying drawings are shown sequentially in the direction of the arrows, these steps are not necessarily executed sequentially in the order indicated by the arrows. Unless there is a clear indication in this document, there is no strict order restriction for the execution of these steps, and they can be executed in other orders. Moreover, at least a part of the steps in the flowchart of the accompanying drawings may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily executed at the same time, but can be executed at different times, and their execution order is not necessarily sequential, but can be executed alternately or in turn with at least a part of other steps or sub-steps or stages of other steps.
Claims
1. A detection method, characterized in that Applied to a microfluidic detection chip, the microfluidic detection chip includes a substrate and a top sealing layer disposed on the upper side of the substrate. A plurality of reagent chambers are spaced apart on the substrate, and a drag channel is provided between two adjacent reagent chambers. The drag channel abuts against the top sealing layer. The method includes: S110: Dispose a first magnetic member above the top sealing layer and a second magnetic member below the substrate; S120: Drive the first magnetic member close to the top sealing layer to gather the magnetic particles in the reagent chamber on one side of the top sealing layer within the reagent chamber; S130: Drive the first magnetic member to move away from the top sealing layer, and at the same time drive the second magnetic member to move close to the bottom of the substrate to adsorb the magnetic particles to the bottom of the reagent chamber; S140: Drive the second magnetic member away from the substrate and drive the first magnetic member close to the top sealing layer to adsorb the magnetic particles to one side of the top sealing layer within the reagent chamber; S150: Drive the first magnetic member and the second magnetic member to move laterally so that the magnetic particles pass through the drag channel and transfer to the next reagent chamber.
2. The control method according to claim 1, characterized in that, After step S150, drive the second magnetic member close to the bottom of the substrate and drive the first magnetic member away from the top sealing layer to adsorb the magnetic particles to the bottom of the reagent chamber.
3. The detection method according to claim 1, characterized in that After step S140, it further includes: controlling the first magnetic member to move laterally so that the magnetic particles move to multiple positions within the reagent chamber.
4. The control method according to any one of claims 1 to 3, characterized in that After step S140, it includes: sequentially repeating step S120, step S130, and step S140 multiple times.
5. The detection method according to claim 1, characterized in that, The substrate is provided with a gas docking hole and a sample chamber. The sample chamber is in fluid communication with the first reagent chamber, and the gas docking hole is in fluid communication with the second reagent chamber. When it is necessary to drive the reagent in the sample chamber to transfer to the first reagent chamber, air is extracted through the gas docking hole so that the reagent chamber presents a negative pressure state.
6. The detection method according to claim 5, characterized in that The substrate is provided with a reaction chamber. The reaction chamber is in fluid communication with the last reagent chamber. When it is necessary to transfer the elution product in the last reagent chamber to the reaction chamber, air is injected into the second reagent chamber through the gas docking hole.
7. The detection method according to claim 6, characterized in that, When the elution product transfers from the reagent chamber to the reaction chamber, control the first magnetic member to be close to the top sealing layer so that the first magnetic member maintains the adsorption effect on the magnetic particles.
8. A microfluidic detection chip, characterized in that, Adopt the detection method described in any one of claims 1 to 7 to transfer the magnetic particles between each cavity.
9. A supporting detection device, characterized in that, It includes: A magnetic assembly including a first magnetic member and a second magnetic member spaced apart; A driving device for driving the first magnetic member to move so that the first magnetic member moves in a direction perpendicular to the microfluidic detection chip, and the driving device is further used to drive the first magnetic member to move laterally along the end face parallel to the microfluidic detection chip; the driving device is used to drive the second magnetic member to move so that the second magnetic member moves in a direction perpendicular to the microfluidic detection chip, and the driving device is further used to drive the second magnetic member to move laterally along the end face parallel to the microfluidic detection chip.
10. The supporting detection device according to claim 9, characterized in that, The supporting detection equipment includes a gas driving control structure. The gas driving control mechanism is used to inhale or blow air into the gas docking hole, and the gas driving control structure is further used to control the on-off of the gas docking hole.