Parallel distributed microfluidic pipeline system

Abstract

The invention discloses a parallel distribution type micro-fluidic pipeline system which comprises a base, a shell is slidably mounted at the top of the base, a micro-fluidic chip is fixedly mounted at the top of the shell, a second linear driving mechanism used for driving the shell is mounted on the base, a rack is fixedly mounted at the top of the base, a first linear driving mechanism used for driving the shell is mounted on the rack, and a second linear driving mechanism used for driving the shell is mounted on the rack. The rack is connected with a trapezoidal block through a first linear driving mechanism; by arranging a first linear driving mechanism, a trapezoidal block, a transmission mechanism, a valve mechanism and a plurality of micro-pipelines, a lysis solution, a cleaning solution, an eluent, a PCR reagent and a diluent which are stored in a first liquid storage tank, a second liquid storage tank, a third liquid storage tank, a fourth liquid storage tank and a fifth liquid storage tank can be sequentially mixed with a sample and the like in a first PCR tube; and liquid in the first PCR tube is pumped into the waste liquid tank or the second PCR tube to synchronously carry out a plurality of times of detection tests, so that the detection efficiency is effectively improved, and the use effect is good.

Description

Technical Field

[0001] The present invention relates to the field of microfluidic technology, in particular to a parallel distributed microfluidic pipeline system. Background Art

[0002] Through miniaturized and integrated design, microfluidic chip technology condenses traditional laboratory functions into centimeter or millimeter-scale chips. Its application areas cover multiple disciplines and scenarios. In the field of biomedical testing, microfluidic chips can automate steps such as sample lysis, mixing, extraction, dilution, and incubation, greatly simplifying the sample preparation process and improving detection efficiency.

[0003] However, most of the microfluidic chips currently used only have a single channel, which is inefficient when batch detection or testing is required. To this end, we propose a parallel distributed microfluidic pipeline system. Summary of the Invention

[0004] The object of the present invention is to provide a parallel distributed microfluidic pipeline system to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A parallel distributed microfluidic pipeline system comprises a base, a shell is slidably mounted on the top of the base, a microfluidic chip is fixedly mounted on the top of the shell, and a second linear drive mechanism for driving the shell is mounted on the base, a frame is fixedly mounted on the top of the base, and a trapezoidal block is connected to the frame through a first linear drive mechanism, the microfluidic chip passes through the inner side of the frame, five valve mechanisms are arranged on the microfluidic chip, five transmission mechanisms are arranged on the frame at the moving path of the trapezoidal block, and the transmission mechanisms correspond to the valve mechanisms one to one, a first liquid reservoir, a second liquid reservoir, a third liquid reservoir, a fourth liquid reservoir and a fifth liquid reservoir are provided on the top surface of the microfluidic chip, a plurality of micro-pipes are provided on the microfluidic chip, and a second flow channel and five third flow channels are provided at the bottom of the microfluidic chip. A flow channel, the liquid inlet end of the valve mechanism is connected to the corresponding first flow channel, and the liquid outlet end of the valve mechanism is connected to the second flow channel. The inner bottoms of the first liquid reservoir, the second liquid reservoir, the third liquid reservoir, the fourth liquid reservoir and the fifth liquid reservoir are each provided with a first through hole, and the bottom end of the first through hole is connected to the corresponding first flow channel, and several micro-pipes are connected to the second flow channel. The microfluidic chip is provided with several first connecting seats, waste liquid tanks and second connecting seats, and the first connecting seat, waste liquid tank and second connecting seat are connected to the corresponding flow channel. The first connecting seat is installed with a first PCR tube, and the second connecting seat is installed with a second PCR tube. The shell is installed with a first air pump, a second air pump, a third air pump and a fourth air pump, and the first air pump, the second air pump, the third air pump and the fourth air pump are all connected to the micro-pipe.

[0007] As a further solution of the present invention: the transmission mechanism includes a guide rod slidably installed on the frame, the outer wall of the guide rod located inside the frame is sleeved with a first spring, the top end of the guide rod is fixedly connected to a force block, and the lower end of the guide rod is fixedly connected to a push rod.

[0008] As a further solution of the present invention: the valve mechanism includes a valve seat fixedly embedded in the bottom of the microfluidic chip, the top of the valve seat is provided with a valve cavity, the bottom of the valve seat is provided with an air vent connected to the valve cavity, and a valve stem is slidably installed at the corresponding position of each valve cavity on the microfluidic chip, the outer wall of the valve stem is sleeved with a second spring, and the lower end of the valve stem is slidably installed in the corresponding valve cavity, the outer wall of the valve stem in the valve cavity is provided with an annular groove, and the outer walls on both sides of the valve seat are respectively provided with a first connecting hole and a second connecting hole connected to the valve cavity, and the bottom of the microfluidic chip and the corresponding positions of each valve seat are provided with a first groove and a second groove, the first groove is connected to the corresponding first flow channel and the first connecting hole, and the second groove is connected to the second flow channel and the corresponding second connecting hole.

[0009] As a further solution of the present invention: the micro-circuit includes a plurality of fifth flow channels, sixth flow channels, ninth flow channels, eleventh flow channels and fourteenth flow channels arranged on the top of the microfluidic chip, a plurality of fourth flow channels, seventh flow channels, tenth flow channels and twelfth flow channels are opened at the bottom of the microfluidic chip, and a plurality of third through holes, fifth through holes, sixth through holes, seventh through holes, eighth through holes, ninth through holes, tenth through holes, eleventh through holes, twelfth through holes, thirteenth through holes, fourteenth through holes and fifteenth through holes are penetrated by the microfluidic chip, two ends of the fourth flow channel are connected to the corresponding third through hole and fifth through hole, two ends of the fifth flow channel are connected to the corresponding fifth through hole and sixth through hole, and two ends of the sixth flow channel are connected to the corresponding seventh through hole and eighth through hole. The two ends of the seventh flow channel are connected with the corresponding eighth through hole and ninth through hole, the two ends of the ninth flow channel are connected with the corresponding tenth through hole and eleventh through hole, the two ends of the tenth flow channel are connected with the corresponding eleventh through hole and twelfth through hole, the twelfth through hole is connected with the corresponding waste liquid tank, the two ends of the eleventh flow channel are connected with the corresponding thirteenth through hole and fourteenth through hole, the two ends of the twelfth flow channel are connected with the corresponding fourteenth through hole and fifteenth through hole, the fifteenth through hole and the second connecting seat are both connected with the corresponding fourteenth flow channel, the sixth through hole, the seventh through hole, the tenth through hole and the thirteenth through hole are all connected with the corresponding first connecting seat, and the tenth through hole and the thirteenth through hole are both equipped with catheters, and the catheters are located in the corresponding first PCR tube.

[0010] As a further solution of the present invention: a third flow channel, an eighth flow channel, a thirteenth flow channel and a fifteenth flow channel are provided on the top of the microfluidic chip, and a first connecting hole, a second connecting hole, a third connecting hole and a fourth connecting hole are provided on the microfluidic chip, the first connecting hole and each third through hole are connected to the third flow channel, the second connecting hole and each waste liquid tank are connected to the fifteenth flow channel, the third connecting hole and each ninth through hole are connected to the eighth flow channel, the fourth connecting hole and each fourteenth flow channel are connected to the thirteenth flow channel, the suction end of the first air pump is connected to the first connecting hole through a hose, the suction end of the second air pump is connected to the second connecting hole through a hose, the suction end of the third air pump is connected to the third connecting hole through a hose, the suction end of the fourth air pump is connected to the fourth connecting hole through a hose, and one-way valves are installed at the third through hole, the ninth through hole, the twelfth through hole and the fifteenth through hole.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] The present invention is equipped with a first linear drive mechanism, a trapezoidal block, a transmission mechanism, a valve mechanism and a plurality of micro-pipelines, so that the lysate, cleaning solution, eluent, PCR reagent and diluent stored in the first liquid reservoir, the second liquid reservoir, the third liquid reservoir, the fourth liquid reservoir and the fifth liquid reservoir can be mixed with the sample in the first PCR tube in sequence, and the liquid in the first PCR tube can be pumped into the waste liquid tank or the second PCR tube to simultaneously perform a plurality of detection tests, thereby effectively improving the detection efficiency and achieving good use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 Schematic diagram of the structure of a parallel distributed microfluidic pipeline system.

[0014] Figure 2 Schematic diagram of the rear structure of a parallel distributed microfluidic pipeline system.

[0015] Figure 3 Schematic diagram of the structure of trapezoidal blocks in a parallel distributed microfluidic pipeline system.

[0016] Figure 4 Schematic diagram of the internal structure of a shell in a parallel distributed microfluidic pipeline system.

[0017] Figure 5 Schematic diagram of the structure of the catheter in a parallel distributed microfluidic pipeline system.

[0018] Figure 6 A cross-sectional view of a microfluidic chip in a parallel distributed microfluidic pipeline system.

[0019] Figure 7 Schematic diagram of the top surface structure of a microfluidic chip in a parallel distributed microfluidic pipeline system.

[0020] Figure 8 This is a schematic diagram of the bottom structure of a microfluidic chip in a parallel distributed microfluidic pipeline system.

[0021] Figure 9 for Figure 8 Enlarged view of point A in the middle.

[0022] Among them, the base 1, the frame 2, the first linear drive mechanism 3, the trapezoidal block 4, the guide rod 5, the force block 6, the first spring 7, the push rod 8, the second linear drive mechanism 9, the shell 10, the microfluidic chip 11, the first liquid reservoir 12, the second liquid reservoir 13, the third liquid reservoir 14, the fourth liquid reservoir 15, the fifth liquid reservoir 16, the first through hole 17, the first flow channel 18, the valve mechanism 19, the valve seat 20, the first groove 21, the second groove 22, the valve cavity 23, the vent 24, the first connecting hole 25, the second connecting hole 26, the valve stem 27, the annular groove 28, the second spring 29, the second flow channel 30, the second through hole 31, the third flow channel 32, the third through hole 33, the fourth flow channel 34, the fifth through hole 35, the fifth flow channel 36, and the sixth through hole 37 , first connecting seat 38, seventh through hole 39, sixth flow channel 40, eighth through hole 41, seventh flow channel 42, ninth through hole 43, eighth flow channel 44, tenth through hole 45, ninth flow channel 46, eleventh through hole 47, tenth flow channel 48, twelfth through hole 49, waste liquid tank 50, thirteenth through hole 51, eleventh flow channel 52, fourteenth through hole 53, twelfth flow channel 54, fifteenth through hole 55, thirteenth flow channel 56, fourteenth flow channel 57, second connecting seat 58, first air pump 59, second air pump 60, third air pump 61, fourth air pump 62, first connecting hole 63, fifteenth flow channel 64, second connecting hole 65, third connecting hole 66, fourth connecting hole 67, conduit 68, first PCR tube 69, second PCR tube 70. DETAILED DESCRIPTION

[0023] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0024] It should be noted that when an element is referred to as being “fixed on” or “disposed on” another element, it may be directly on the other element or indirectly on the other element. When an element is referred to as being “connected to” another element, it may be directly connected to the other element or indirectly connected to the other element.

[0025] It should be understood that the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation in the specification, and therefore cannot be understood as limiting the present invention.

[0026] Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of the features.

[0027] See also Figures 1 to 9 In an embodiment of the present invention, a parallel distributed microfluidic pipeline system includes a base 1, a housing 10 is slidably mounted on the top of the base 1, a microfluidic chip 11 is fixedly mounted on the top of the housing 10, and the microfluidic chip 11 is composed of a substrate made of PDMS or PMMA material and a plurality of glass plates. The glass plates are embedded on both sides of the substrate to seal the flow channel on the substrate, and a second linear drive mechanism 9 for driving the housing 10 is installed on the base 1, and a frame 2 is fixedly mounted on the top of the base 1, and the frame 2 is fixedly mounted on the first linear drive mechanism 9. The mechanism 3 is connected to the trapezoidal block 4. The first linear drive mechanism 3 is composed of a screw and a ball screw nut driven by a servo motor and a guide rail. The microfluidic chip 11 passes through the inner side of the frame 2. Five valve mechanisms 19 are provided on the microfluidic chip 11. Five transmission mechanisms are provided on the frame 2 at the moving path of the trapezoidal block 4, and the transmission mechanisms correspond to the valve mechanisms 19 one by one. The top surface of the microfluidic chip 11 is provided with a first liquid reservoir 12, a second liquid reservoir 13, a third liquid reservoir 14, a fourth liquid reservoir 15 and a fifth liquid reservoir 16. The microfluidic chip 11 is provided with several micro-channels, and a second flow channel 30 and five first flow channels 18 are opened at the bottom of the microfluidic chip 11. The liquid inlet end of the valve mechanism 19 is connected to the corresponding first flow channel 18, and the liquid outlet end of the valve mechanism 19 is connected to the second flow channel 30. The inner bottoms of the first liquid reservoir 12, the second liquid reservoir 13, the third liquid reservoir 14, the fourth liquid reservoir 15 and the fifth liquid reservoir 16 are all provided with a first through hole 17, and the bottom end of the first through hole 17 is connected to the corresponding first flow channel 18. Several of the micro-channels are connected to the second flow channel 30. The microfluidic chip 11 is provided with several first connecting seats 38, waste liquid tanks 50 and second connecting seats 58. The first connecting seats 38, waste liquid tanks 50 and second connecting seats 58 are connected to the corresponding flow channels. The first connecting seats 38 are each installed with a first PCR tube 69, and the second connecting seats 58 are each installed with a second PCR tube 70. The shell 10 is installed with a first air pump 59, a second air pump 60, a third air pump 61 and a fourth air pump 62. The first air pump 59, the second air pump 60, the third air pump 61 and the fourth air pump 62 are all connected to the micro pipeline.

[0028] By adopting the above scheme, the present invention can store lysate, cleaning solution, eluent, PCR reagent and diluent in the first liquid reservoir 12, the second liquid reservoir 13, the third liquid reservoir 14, the fourth liquid reservoir 15 and the fifth liquid reservoir 16 respectively during use, and place samples, positive controls, negative controls and quality control products in each first PCR tube 69 respectively. After that, the trapezoidal block 4 is driven to move by the first linear drive mechanism, and the corresponding valve mechanism 19 can be opened by the transmission mechanism to drive the lysate to be diverted to the corresponding first micro-channels in parallel under the action of the air pump. The waste liquid is then transported to the waste liquid tank 50 through the micro-pipeline under the action of the air pump, and the valve mechanism 19 is opened in sequence to mix the cleaning liquid, eluent, PCR reagent and diluent with the sample in sequence, and finally the DNA is extracted. The DNA is transported to the second PCR tube 70 through the air pump and mixed with the probe to perform PCR reaction and real-time fluorescence detection at the same time. Through the setting of multiple micro-pipelines, several detection tests can be carried out simultaneously, thereby effectively improving the detection efficiency and achieving good use effect.

[0029] Specific combination Figure 3 In one embodiment of the present invention, the transmission mechanism includes a guide rod 5 slidably installed on the frame 2, the outer wall of the guide rod 5 located inside the frame 2 is sleeved with a first spring 7, the top end of the guide rod 5 is fixedly connected to a force block 6, and the lower end of the guide rod 5 is fixedly connected to a push rod 8.

[0030] When the trapezoidal block 4 contacts the force-bearing block 6 under the drive of the first linear drive mechanism, it will push the corresponding guide rod 5 and the push rod 8 to move downward to open the valve mechanism. When the trapezoidal block 4 is separated from the force-bearing block 6, the force-bearing block 6 will be reset upward under the action of the first spring 7 to close the corresponding valve mechanism.

[0031] Specific combination Figure 3 、 Figure 6 and Figure 9On the basis of the previous embodiment, the valve mechanism 19 further comprises a valve seat 20 fixedly embedded in the bottom of the microfluidic chip 11, a valve cavity 23 is formed on the top of the valve seat 20, and a vent 24 communicating with the valve cavity 23 is formed on the bottom of the valve seat 20. A valve stem 27 is slidably mounted at the corresponding position of each valve cavity 23 on the microfluidic chip 11, and the outer wall of the valve stem 27 is provided with a second spring 29, and the lower end of the valve stem 27 is slidably mounted on the corresponding valve cavity 23. 3, an annular groove 28 is formed on the outer wall of the valve stem 27 located in the valve cavity 23, and a first communicating hole 25 and a second communicating hole 26 communicating with the valve cavity 23 are respectively formed on the outer walls of both sides of the valve seat 20, and a first groove 21 and a second groove 22 are formed at the bottom of the microfluidic chip 11 and at the corresponding positions of each valve seat 20, the first groove 21 is communicated with the corresponding first flow channel 18 and the first communicating hole 25, and the second groove 22 is communicated with the second flow channel 30 and the corresponding second communicating hole 26.

[0032] When the valve stem 27 is pressed downward by the corresponding push rod 8, it will move downward in the valve chamber 23 so that the annular groove 28 is aligned with the first connecting hole 25 and the second connecting hole 26. At this time, the first connecting hole 25 and the second connecting hole 26 will be connected through the annular groove 28, so that the first flow channel 18 is connected with the second flow channel 30, thereby realizing the transportation of liquid under the action of the air pump.

[0033] Specific combination Figure 5 、 Figure 7 and Figure 8In one embodiment of the present invention, the micro-channels include a plurality of fifth flow channels 36, a sixth flow channel 40, a ninth flow channel 46, an eleventh flow channel 52, and a fourteenth flow channel 57 arranged on the top of the microfluidic chip 11, a plurality of fourth flow channels 34, a seventh flow channel 42, a tenth flow channel 48, and a twelfth flow channel 54 are opened on the bottom of the microfluidic chip 11, and a plurality of third through holes 33, a fifth through hole 35, a sixth through hole 37 are drilled on the microfluidic chip 11. , seventh through hole 39, eighth through hole 41, ninth through hole 43, tenth through hole 45, eleventh through hole 47, twelfth through hole 49, thirteenth through hole 51, fourteenth through hole 53 and fifteenth through hole 55, both ends of the fourth flow channel 34 are connected to the corresponding third through hole 33 and fifth through hole 35, both ends of the fifth flow channel 36 are connected to the corresponding fifth through hole 35 and sixth through hole 37, both ends of the sixth flow channel 40 are connected to the corresponding seventh through hole 39 and eighth through hole 41 is connected, both ends of the seventh flow channel 42 are connected with the corresponding eighth through hole 41 and ninth through hole 43, both ends of the ninth flow channel 46 are connected with the corresponding tenth through hole 45 and eleventh through hole 47, both ends of the tenth flow channel 48 are connected with the corresponding eleventh through hole 47 and twelfth through hole 49, the twelfth through hole 49 is connected with the corresponding waste liquid tank 50, both ends of the eleventh flow channel 52 are connected with the corresponding thirteenth through hole 51 and fourteenth through hole 53, both ends of the twelfth flow channel 54 are connected with the corresponding fourteenth through hole 53 and fifteenth through hole 55, the fifteenth through hole 55 and the second connecting seat 58 are both connected with the corresponding fourteenth flow channel 57, the sixth through hole 37, the seventh through hole 39, the tenth through hole 45 and the thirteenth through hole 51 are all connected with the corresponding first connecting seat 38, and a conduit 68 is installed in the tenth through hole 45 and the thirteenth through hole 51, and the conduit 68 is located in the corresponding first PCR tube 69.

[0034] Furthermore, the top of the microfluidic chip 11 is provided with a third flow channel 32, an eighth flow channel 44, a thirteenth flow channel 56 and a fifteenth flow channel 64, and the microfluidic chip 11 is provided with a first connection hole 63, a second connection hole 65, a third connection hole 66 and a fourth connection hole 67. The first connection hole 63 and each third through hole 33 are connected to the third flow channel 32, the second connection hole 65 and each waste liquid tank 50 are connected to the fifteenth flow channel 64, and the third connection hole 66 and each ninth through hole 43 are connected to the eighth flow channel 44. The fourth connecting hole 67 and each fourteenth flow channel 57 are connected to the thirteenth flow channel 56, the suction end of the first air pump 59 is connected to the first connecting hole 63 through a hose, the suction end of the second air pump 60 is connected to the second connecting hole 65 through a hose, the suction end of the third air pump 61 is connected to the third connecting hole 66 through a hose, and the suction end of the fourth air pump 62 is connected to the fourth connecting hole 67 through a hose, and the third through hole 33, the ninth through hole 43, the twelfth through hole 49 and the fifteenth through hole 55 are all installed with a one-way valve.

[0035] When the first air pump 59 is started, the liquid in the first liquid reservoir 12 to the fifth liquid reservoir 16 is input into the third liquid channel 32 through the first through hole 17, the first flow channel 18, the second flow channel 30 and the second through hole 31 when the valve mechanism 19 is in the open state;

[0036] When the third air pump 61 is activated, the gas in each first PCR tube 69 is pumped out through the eighth flow channel 44, the ninth through hole 43, the seventh flow channel 42, the eighth through hole 41, the sixth flow channel 40, and the seventh through hole 39. The negative pressure generated in the first PCR tube 69 then pumps the liquid in the third flow channel 32 into the first PCR tube 69 through the third through hole 33, the fourth flow channel 34, the fifth through hole 35, the fifth flow channel 36, and the sixth through hole 37 to mix with the substance in the first PCR tube 69.

[0037] When the second air pump 60 is activated, the gas in each waste liquid tank 50 is pumped out through the second connecting hole 65 and the fifteenth flow channel 64. The negative pressure is then used to pump the liquid in the first PCR tube 69 into the corresponding waste liquid tank 50 through the twelfth through hole 49, the tenth flow channel 48, the eleventh through hole 47, the ninth flow channel 46, the tenth through hole 45, and the corresponding conduit 68.

[0038] By starting the fourth air pump 62, the gas in each second connecting seat 58 can be extracted through the fourth connecting hole 67 and the thirteenth flow channel 56, so as to draw the liquid in the first PCR tube 69 into the corresponding second PCR tube 70 through the second connecting seat 58, the fifteenth through hole 55, the twelfth flow channel 54, the fourteenth through hole 53, the eleventh flow channel 52, the thirteenth through hole 51 and the corresponding conduit 68.

[0039] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A parallel distributed microfluidic pipeline system, characterized by: The invention comprises a base (1), a housing (10) is slidably mounted on the top of the base (1), a microfluidic chip (11) is fixedly mounted on the top of the housing (10), and a second linear drive mechanism (9) for driving the housing (10) is mounted on the base (1), a frame (2) is fixedly mounted on the top of the base (1), and a trapezoidal block (4) is connected to the frame (2) via a first linear drive mechanism (3), the microfluidic chip (11) passes through the inner side of the frame (2), and five valve mechanisms (19) are provided on the microfluidic chip (11). The frame (2) is provided with five transmission mechanisms at the moving path of the trapezoidal block (4), and the transmission mechanisms correspond to the valve mechanisms (19) one by one. The top surface of the microfluidic chip (11) is provided with a first liquid reservoir (12), a second liquid reservoir (13), a third liquid reservoir (14), a fourth liquid reservoir (15) and a fifth liquid reservoir (16). The microfluidic chip (11) is provided with a plurality of micro-channels. The bottom of the microfluidic chip (11) is provided with a second flow channel (30) and five first flow channels (18). The liquid inlet end of the valve mechanism (19) is connected to the corresponding valve mechanism (19). The first flow channel (18) is connected to the corresponding one, the liquid outlet end of the valve mechanism (19) is connected to the second flow channel (30), the first liquid storage tank (12), the second liquid storage tank (13), the third liquid storage tank (14), the fourth liquid storage tank (15) and the fifth liquid storage tank (16) are all provided with a first through hole (17) on the inner bottom, and the bottom end of the first through hole (17) is connected to the corresponding one. The micro-pipes are all connected to the second flow channel (30), and the microfluidic chip (11) is provided with a plurality of first connecting seats (38), waste liquid tanks (50) and second The connecting seat (58) is connected to the corresponding flow channel. The first connecting seat (38), the waste liquid tank (50) and the second connecting seat (58) are connected to the corresponding flow channel. The first PCR tube (69) is installed on the first connecting seat (38), and the second PCR tube (70) is installed on the second connecting seat (58). The first air pump (59), the second air pump (60), the third air pump (61) and the fourth air pump (62) are installed on the shell (10). The first air pump (59), the second air pump (60), the third air pump (61) and the fourth air pump (62) are all connected to the micro-pipeline.

2. The parallel distributed microfluidic pipeline system according to claim 1, characterized in that: The transmission mechanism comprises a guide rod (5) slidably mounted on a frame (2); the outer wall of the guide rod (5) located inside the frame (2) is sleeved with a first spring (7); the top end of the guide rod (5) is fixedly connected to a force block (6); and the lower end of the guide rod (5) is fixedly connected to a push rod (8).

3. The parallel distributed microfluidic pipeline system according to claim 2, characterized in that: The valve mechanism (19) includes a valve seat (20) fixedly embedded in the bottom of the microfluidic chip (11), a valve cavity (23) is provided on the top of the valve seat (20), and a vent hole (24) connected to the valve cavity (23) is provided on the bottom of the valve seat (20). A valve stem (27) is slidably installed at a corresponding position of each valve cavity (23) on the microfluidic chip (11), an outer wall of the valve stem (27) is provided with a second spring (29), and the lower end of the valve stem (27) is slidably installed in the corresponding valve cavity (23). An annular groove (28) is provided on the outer wall inside the valve cavity (23), and a first connecting hole (25) and a second connecting hole (26) are respectively provided on the outer walls on both sides of the valve seat (20) and are connected to the valve cavity (23). A first groove (21) and a second groove (22) are provided at the bottom of the microfluidic chip (11) and at the corresponding positions of each valve seat (20), the first groove (21) is connected to the corresponding first flow channel (18) and the first connecting hole (25), and the second groove (22) is connected to the second flow channel (30) and the corresponding second connecting hole (26).

4. The parallel distributed microfluidic pipeline system according to claim 1, characterized in that: The micro-channels include a plurality of fifth flow channels (36), a sixth flow channel (40), a ninth flow channel (46), an eleventh flow channel (52) and a fourteenth flow channel (57) arranged on the top of the microfluidic chip (11); a plurality of fourth flow channels (34), a seventh flow channel (42), a tenth flow channel (48) and a twelfth flow channel (54) are opened on the bottom of the microfluidic chip (11); a plurality of third through holes (33), a fifth through hole (35), a sixth through hole (37), a seventh through hole (39), a fourth through hole (40), a fifth through hole (41), a sixth through hole (42), a seventh through hole (43), a seventh through hole (44), a seventh through hole (45), a seventh through hole (46), a seventh through hole (47), a seventh through hole (48) and a twelfth through hole (54) are penetrated on the microfluidic chip (11); The eighth through hole (41), the ninth through hole (43), the tenth through hole (45), the eleventh through hole (47), the twelfth through hole (49), the thirteenth through hole (51), the fourteenth through hole (53) and the fifteenth through hole (55), the two ends of the fourth flow channel (34) are connected to the corresponding third through hole (33) and the fifth through hole (35), the two ends of the fifth flow channel (36) are connected to the corresponding fifth through hole (35) and the sixth through hole (37), the two ends of the sixth flow channel (40) are connected to the corresponding seventh through hole (39) and the eighth through hole (41). The seventh flow channel (42) is connected to the corresponding eighth through hole (41) and the ninth through hole (43), the ninth flow channel (46) is connected to the corresponding tenth through hole (45) and the eleventh through hole (47), the tenth flow channel (48) is connected to the corresponding eleventh through hole (47) and the twelfth through hole (49), the twelfth through hole (49) is connected to the corresponding waste liquid tank (50), the eleventh flow channel (52) is connected to the corresponding thirteenth through hole (51) and the fourteenth through hole (53), the tenth through hole (54) is connected to the corresponding waste liquid tank (50), the tenth through hole (55) is connected to the corresponding thirteenth through hole (51) and the fourteenth through hole (53), the tenth through hole (56) is connected to the corresponding waste liquid tank (50), the tenth through hole (57) is connected to the corresponding thirteenth through hole (51) and the fourteenth through hole (53), the tenth through hole (58) is connected to the corresponding thirteenth through hole (51) and the fourteenth through hole (53), the tenth through hole (59) is connected to the corresponding waste liquid tank (50), the tenth through hole (59) is connected to the corresponding waste liquid tank (50), the tenth through hole (51) is connected to the corresponding thirteenth through hole (51) and the fourteenth through hole (53), the tenth through hole (59) is connected to the corresponding waste liquid tank (50), the tenth through hole (59) is connected to the corresponding waste liquid tank (50), the tenth through hole (5 The two ends of the second flow channel (54) are connected to the corresponding fourteenth through hole (53) and the fifteenth through hole (55), the fifteenth through hole (55) and the second connecting seat (58) are both connected to the corresponding fourteenth flow channel (57), the sixth through hole (37), the seventh through hole (39), the tenth through hole (45) and the thirteenth through hole (51) are all connected to the corresponding first connecting seat (38), and a conduit (68) is installed in the tenth through hole (45) and the thirteenth through hole (51), and the conduit (68) is located in the corresponding first PCR tube (69).

5. The parallel distributed microfluidic pipeline system according to claim 4, characterized in that: The top of the microfluidic chip (11) is provided with a third flow channel (32), an eighth flow channel (44), a thirteenth flow channel (56) and a fifteenth flow channel (64); the microfluidic chip (11) is provided with a first connection hole (63), a second connection hole (65), a third connection hole (66) and a fourth connection hole (67); the first connection hole (63) and each third through hole (33) are in communication with the third flow channel (32); the second connection hole (65) and each waste liquid tank (50) are in communication with the fifteenth flow channel (64); the third connection hole (66) and each ninth through hole (43) are in communication with the eighth flow channel (44); The fourth connecting hole (67) and each of the fourteenth flow channels (57) are connected to the thirteenth flow channel (56); the suction end of the first air pump (59) is connected to the first connecting hole (63) through a hose; the suction end of the second air pump (60) is connected to the second connecting hole (65) through a hose; the suction end of the third air pump (61) is connected to the third connecting hole (66) through a hose; the suction end of the fourth air pump (62) is connected to the fourth connecting hole (67) through a hose; and one-way valves are installed at the third through hole (33), the ninth through hole (43), the twelfth through hole (49) and the fifteenth through hole (55).

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