Fusarium equisetifolia RT199, fermentation agent and application thereof

Abstract

The present invention provides a Fusarium equisetifolia RT199, a fermentation agent, and applications thereof, belonging to the field of microbial technology. The present invention provides a Fusarium equisetifolia RT199, with a deposit number of CGMCC No. 41794. The Fusarium equisetifolia RT199 has the ability to secrete cellulase, laccase, and xylanase; the Fusarium equisetifolia RT199 has the effect of efficiently degrading straw and improving soil. The results of the embodiment show that the degradation rate of the Fusarium equisetifolia RT199 on corn straw at room temperature of 25-30°C reaches 70.53%. The Fusarium equisetifolia RT199 is inoculated on the straw, and the straw inoculated with the agent is then returned to the field. After 30 days of returning to the field, the soil POC content increased by 19.16%, MAOC increased by 39.59%, and the soil total organic carbon content increased by 31.99% compared with the control group.

Description

Technical Field

[0001] The present invention belongs to the technical field of microorganisms, and in particular relates to Fusarium equisetifolia RT199, a fermentation agent and applications thereof. Background Art

[0002] As a renewable biomass resource, the efficient utilization of straw is crucial for sustainable agricultural development. Direct in-situ incorporation into the field is one approach to utilizing straw resources. Theoretically, straw can be converted into organic fertilizer, replenishing soil organic matter (SOM), improving soil aggregate structure, and enhancing soil fertility. However, straw is composed of cellulose (35%-50%), hemicellulose (15%-30%), and lignin (10%-25%) cross-linked through a complex three-dimensional network, making it highly resistant to degradation. Under natural conditions, straw decomposes slowly, with a half-life of 6-12 months. This leads to significant problems, including: stunted crop growth: Incompletely decomposed straw residue in the soil can hinder seed germination and root development, resulting in a 10%-15% decrease in seedling emergence and, in severe cases, seedling burn; and difficult tillage management: excessive straw coverage increases the resistance of agricultural machinery by 20%-30%, significantly reducing the efficiency of operations such as sowing and fertilization.

[0003] Currently, straw degradation relies primarily on chemical treatment (such as strong acid and alkali pretreatment) or high-temperature microbial inoculants (optimal temperature 40-60°C). The former poses environmental pollution risks and disrupts soil microbial communities; the latter experiences a sharp decline in activity during the low temperatures of winter (-20-10°C) or early spring in northern China, making efficient year-round degradation difficult. Furthermore, traditional microbial inoculants have limited effects on soil carbon pool regulation, particularly on mineral-associated organic carbon (MAOC)—a key component of long-term soil carbon sequestration—with reported increases generally below 15%. Therefore, developing a highly efficient straw-degrading microbial inoculant at room temperature that combines rapid decomposition with soil carbon optimization has become a critical technical bottleneck in the field of agricultural waste resource utilization and improved farmland quality. Summary of the Invention

[0004] In view of the defects in the prior art, the purpose of the present invention is to provide a Fusarium equisetifolia RT199 that can efficiently degrade straw at room temperature, increase the soil carbon pool content, and thus promote crop yield.

[0005] The purpose of the present invention is achieved through the following technical solutions:

[0006] The present invention provides a kind of Fusarium equisetifolia ( Fusarium equiseti ) RT199, the preservation number of the Fusarium equisetifolia RT199 is CGMCC No. 41794.

[0007] The present invention provides a fermentation bacterial agent, comprising the Fusarium equisetifolia RT199 described in the above technical solution.

[0008] The present invention provides a method for preparing the fermentation agent described in the above technical solution, comprising:

[0009] The Fusarium equisetifolia RT199 is cultured in a culture medium to obtain a fermentation agent.

[0010] Preferably, the culture temperature is 25-30° C. and the culture time is 4-7 days.

[0011] The present invention provides an application of the Fusarium equisetifolia RT199 described in the above technical solution, the fermentation agent described in the above technical solution, or the fermentation agent prepared by the preparation method described in the above technical solution in straw degradation.

[0012] The present invention provides a method for degrading straw, comprising:

[0013] The Fusarium equisetifolia RT199 described in the above technical solution is inoculated into straw for fermentation.

[0014] Preferably, the fermentation temperature is 25-30° C.; and the fermentation time is ≥30 days.

[0015] Preferably, during inoculation, the mass ratio of the dry weight of the straw to the fungal mycelium of Fusarium equisetifolia RT199 is 15:1 to 30:1.

[0016] Preferably, the method for preparing the fungal mycelium comprises:

[0017] Cultivating the Fusarium equisetifolia RT199 in a culture medium to obtain a fungus activation solution;

[0018] Fungal mycelium is separated from the fungus activation liquid.

[0019] The present invention provides the Fusarium equisetifolia RT199 described in the above technical solution, the fermentation agent described in the above technical solution, the fermentation agent prepared by the preparation method described in the above technical solution, or the use of the method described in the above technical solution in improving soil.

[0020] Beneficial effects of the present invention:

[0021] The present invention provides a kind of Fusarium equisetifolia ( Fusarium equiseti) RT199, the deposit number of Fusarium equiseti RT199 is CGMCC No. 41794. The Fusarium equiseti RT199 provided by the present invention has the ability to secrete cellulases, laccases, and xylanases; it is also highly effective in degrading straw and improving soil. Examples of the present invention show that Fusarium equiseti RT199 can degrade corn straw at a rate of 70.53% at room temperature (25-30°C). When Fusarium equiseti RT199 was inoculated onto straw and then returned to the field, 30 days after return, the soil POC content increased by 19.16%, MAOC by 39.59%, and total organic carbon by 31.99% compared to the control group. The Fusarium equiseti RT199 provided by the present invention is suitable for the resource utilization of agricultural waste such as straw and soil improvement, thereby improving the efficiency of agricultural waste resource utilization and enhancing arable land quality.

[0022] Biological Deposit Description

[0023] Fusarium equisetifolia RT199, taxonomically designated as: Fusarium equiseti On March 10, 2025, it was deposited in the General Microbiology Center of China Culture Collection Administration, located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No.41794. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 This is a diagram showing the results of enzyme activity assays of the strains screened in Example 1;

[0026] Figure 2 This is the result of measuring the corn straw degradation rate of the strains screened under liquid fermentation conditions;

[0027] Figure 3 This is the result of corn straw degradation rate in the buried bag experiment of RT199 strain;

[0028] Figure 4 This is the result of the effect of RT199 strain on soil POC and MAOC after straw returned to the field;

[0029] Figure 5 The phylogenetic tree and microscopic morphology of the RT199 strain are shown;

[0030] Figure 6 This is the morphological observation result of corn straw after degradation by RT199 strain. DETAILED DESCRIPTION

[0031] The present invention provides a kind of Fusarium equisetifolia ( Fusarium equiseti ) RT199, the preservation number of the Fusarium equisetifolia RT199 is CGMCC No. 41794.

[0032] The Fusarium equiseti RT199 provided by the present invention was isolated from the roots of healthy corn in the farmland of Qiqihar City. The ITS sequence of the Fusarium equiseti RT199 is shown in SEQ ID NO.3. The Fusarium equiseti RT199 has the ability to secrete cellulase, laccase and xylanase. In the present invention, the Fusarium equiseti RT199 can degrade straw. The results of the examples of the present invention show that the degradation rate of corn straw within 30 days at room temperature using the Fusarium equiseti RT199 is as high as 70.53%. The degradation effect of the Fusarium equiseti RT199 on straw is significantly better than that of Bacillus cereus W118. In the present invention, the Fusarium equiseti RT199 has the effect of improving soil. The results of the present invention show that when the Fusarium equisetifolia RT199 is inoculated into straw and then the inoculated straw is returned to the field, 30 days after returning to the field, the soil POC content increases by 19.16%, MAOC increases by 39.59%, and the soil total organic carbon content increases by 31.99% compared with the control group.

[0033] The present invention provides a fermentation bacterial agent, comprising the Fusarium equisetifolia RT199 described in the above technical solution.

[0034] The present invention provides a method for preparing the fermentation agent described in the above technical solution, comprising: culturing the Fusarium equiseti RT199 in a culture medium to obtain the fermentation agent. As an optional embodiment of the present invention, the culture medium may be PDA medium, PDB medium, or a liquid fermentation medium. The present invention does not specifically limit the composition of the liquid fermentation medium; any conventional culture medium composition in the art that enables normal growth of Fusarium equiseti RT199 to produce fungal mycelium may be used. As an optional embodiment of the present invention, the culture temperature may be 25-30°C, or may be 25, 26, 27, 28, 29, or 30°C; the culture duration may be 4-7 days, or may be 4, 5, 6, or 7 days; the culture is preferably accompanied by shaking; and the shaking speed may be 150 rpm. After completion of the culture, a culture solution is obtained. As an optional embodiment of the present invention, the culture solution may be used directly as the fermentation agent. As another optional embodiment of the present invention, the fungal mycelium in the culture solution may be further separated and used as the fermentation agent. The present invention does not specifically limit the separation method; any conventional separation method in the art may be used. As an optional embodiment of the present invention, the separation method can be centrifugation or filtration; when centrifuging, the centrifuge speed can be 8000 rpm, and the centrifugation time can be 10 minutes. After isolating the fungal mycelium, the present invention preferably resuspends the fungal mycelium in sterile water to obtain a bacterial suspension. The present invention can also use the bacterial suspension as a fermentation agent. The present invention can directly use the fermentation agent to degrade straw, thereby improving soil.

[0035] The present invention provides the use of the Fusarium equisetifolia RT199 described in the above technical solution, the fermentation agent described in the above technical solution, or the fermentation agent prepared by the preparation method described in the above technical solution for straw degradation. As an optional embodiment of the present invention, the straw comprises corn straw. The present invention demonstrates that the straw degradation rate of the Fusarium equisetifolia RT199 is as high as 70.53% within 30 days at room temperature.

[0036] The present invention provides a method for degrading straw, comprising: inoculating the Fusarium equiseti RT199 described in the above technical solution into straw for fermentation. In the present invention, the Fusarium equiseti RT199 is preferably activated before degrading straw, or the Fusarium equiseti RT199 is preferably prepared into a fermentation agent. Activating the Fusarium equiseti RT199 is one method for preparing the fermentation agent. The present invention does not specifically limit the method for activating the Fusarium equiseti RT199, and any conventional method in the art can be used. As an optional embodiment of the present invention, the method for activating the Fusarium equiseti RT199 comprises inoculating the Fusarium equiseti RT199 into PDB medium and culturing it to obtain an activated fungus solution. In the present invention, the culturing temperature can be 25-30°C, or can be 25, 26, 27, 28, 29, or 30°C; the culturing time can be 4-7 days, or can be 4, 5, 6, or 7 days; the culturing process is preferably accompanied by shaking, and the shaking speed can be 150 rpm. After obtaining the fungus activation liquid, the present invention preferably separates the fungus mycelium in the fungus activation liquid, and after resuspending the fungus mycelium, obtains a fungus mycelium suspension. As an optional embodiment of the present invention, the fungus mycelium suspension may contain 0.5g of fungus mycelium per 100mL of the suspension. The present invention preferably inoculates the fungus mycelium suspension into straw for fermentation. The present invention does not particularly limit the inoculation method, and any conventional inoculation method in the art may be adopted. As an optional embodiment of the present invention, the inoculation method of the fungus mycelium may be to directly mix the fungus mycelium suspension with straw, or the inoculation method may be to apply straw to the soil, and then apply the fungus mycelium suspension to the soil, and the fungus mycelium suspension reaches the surface of the straw through natural infiltration, thereby degrading the straw. As an optional embodiment of the present invention, when inoculating, the mass ratio of the dry weight of the straw to the fungal mycelium of the Fusarium equisetifolia RT199 can be 15:1~30:1, or 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1 or 30:1. In the present invention, the fermentation temperature can be field and / or room temperature. As an optional embodiment of the present invention, the fermentation temperature can be 25~30°C, or 25, 26, 27, 28, 29 or 30°C; the fermentation time is ≥30d, and can be 30~40d, or 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40d. In the present invention, returning the straw to the field is a way to degrade the straw.When returning straw to the field, the present invention can directly irrigate the soil after returning the straw to the field with a bacterial suspension containing the fungal mycelium, and then degrade the straw under normal temperature conditions. When returning straw to the field, the present invention can also mix the bacterial suspension containing the fungal mycelium with the straw, and then return the straw inoculated with the bacterial agent to the field to degrade the straw.

[0037] The present invention also provides the use of the Fusarium equiseti RT199 described in the above technical solution, the fermentation agent described in the above technical solution, the fermentation agent prepared by the preparation method described in the above technical solution, or the method described in the above technical solution in improving soil. In the present invention, the Fusarium equiseti RT199 has the effect of improving soil. In the present invention, the Fusarium equiseti RT199 is inoculated into straw after returning to the field and fermented for 30 days. After returning to the field, the POC content of the soil increased by 19.16% compared with the control, the MAOC increased by 39.59% compared with the control, and the total organic carbon content of the soil increased by 31.99% compared with the control.

[0038] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0039] Example 1

[0040] Strain screening

[0041] 1. Strain Isolation: Eleven fungal strains were isolated and purified from healthy corn roots grown in farmland in Qiqihar City. The strains were named RT32, RT46, RT208, RT161, RT199, RT22, RT4, RT78, RT47, RT181, and RT154. The culture medium used for isolation and purification was potato dextrose agar (PDA).

[0042] 2. Initial screening of strains (enzyme activity determination):

[0043] Strains RT32, RT46, RT208, RT161, RT199, RT22, RT4, RT78, RT47, RT181, and RT154 were activated on PDA plates. A 1-cm diameter sterile hole was used to remove the bacterial cake from the PDA plates and inoculated into enzyme-producing medium (medium formulation: CMC-Na 5.0 g / L, peptone 3.0 g / L, MgSO₄·7H₂O 0.3 g / L, yeast extract 0.5 g / L, CaCl₂·2H₂O 0.3 g / L, KH₂PO₄ 1.5 g / L; pH 7.0–7.3). Cultures were maintained at 28°C for 10 days with shaking at 180 rpm. The supernatant was centrifuged and filtered through a 0.22 μm filter to remove any residual bacteria or particulate matter. The activities of cellulase, laccase and xylanase in the culture supernatant were detected using the following method.

[0044] Cellulase activity: The DNS method (3,5-dinitrosalicylic acid method) was used with sodium carboxymethyl cellulose (CMC-Na) as the substrate. The reaction was carried out at 37°C for 1 h to determine the amount of reducing sugar produced.

[0045] Laccase activity: The ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) method was used to measure the absorbance change (λ = 420 nm). The reaction conditions were 60°C for 20 min.

[0046] Xylanase activity: Using birchwood xylan as substrate, the amount of reducing sugar released was determined by the DNS method. The reaction conditions were 50°C and lasted for 30 min.

[0047] The results of enzyme production test of strains RT32, RT46, RT208, RT161, RT199, RT22, RT4, RT78, RT47, RT181 and RT154 are shown in Table 1 and Figure 1 shown.

[0048] Table 1 Enzyme production of each strain

[0049]

[0050] From Table 1 and Figure 1 The results showed that strains RT32, RT46, RT208, RT161, RT199, RT22, and RT4 had high degradation enzyme activities. Among them, the xylanase and cellulase activities of strain RT199 were significantly higher than those of the other strains. The enzyme activities of strains RT32, RT46, RT208, RT161, RT22, and RT4 were also relatively high. Therefore, strains RT32, RT46, RT208, RT161, RT199, RT22, and RT4 were subsequently rescreened.

[0051] 3. Strain rescreening

[0052] (1) Preparation of liquid fermentation medium: Crush corn straw to less than 2 mm and add it to the basal culture medium at 5 g / L. The formula of 1 L basal culture medium is shown in Table 2.

[0053] Table 2 Basic culture medium formula

[0054]

[0055] After the culture medium was prepared, the pH was adjusted to 6.5 with NaOH and sterilized at 121°C for 15 min.

[0056] (2) Seven fungal strains with high enzyme activity, including RT32, RT46, RT208, RT161, RT199, RT22 and RT4, were selected for rescreening.

[0057] Seven fungal strains, RT32, RT46, RT208, RT161, RT199, RT22 and RT4, were cultured on PDA plates at 25°C for 7 days.

[0058] Use a 1-cm-diameter borer to extract three fungal cakes from the PDA plate of each fungal strain. Inoculate these three cakes into one bottle of liquid fermentation medium and incubate at 25–30°C, shaking at 150 rpm for 30 days. Inoculate an equal amount of PDA agar cakes into one bottle of liquid fermentation medium as a negative control.

[0059] (3) After 30 days of cultivation, the residual straw was collected and dried at 60°C to constant weight. The weight loss rate was calculated according to the formula: weight loss rate = (initial weight - residual weight) / initial weight × 100%.

[0060] (4) The results of corn straw weight loss rate after 30 days of liquid fermentation are as follows: Figure 2 shown.

[0061] Depend on Figure 2 The results showed that the degradation rates of corn straw for strains RT32, RT46, RT208, RT161, RT199, RT22, and RT4 were significantly higher than those for RT22 (37.5%), RT199 (24.5%), and RT46 (17.0%) within 30 days, respectively. RT208 and RT161 had lower degradation rates of only 2.32% and 5.62%, respectively.

[0062] Example 2

[0063] Soil bag experiment on RT199 and RT22

[0064] 1. Wash the corn stalks (stems and leaves mixed), dry them in an oven at 50℃ for 8 hours until constant weight is reached, cut them into lengths of about 5 cm, weigh the corn stalks and put them into nylon mesh bags, with 1.5 g of corn stalks in each bag.

[0065] 2. Place 300g of soil (black soil, initial organic matter 20g / kg) in a flowerpot. Place a nylon mesh bag filled with corn stalks in the flowerpot and cover with 200g of soil, burying the nylon mesh bag approximately 5cm deep in the flowerpot. Add 100mL of sterile water from the tray to the buried flowerpot, allowing the soil to fully absorb water and reach a humidity of 60%-65%.

[0066] 3. Bacteria activation:

[0067] The fungal strains were cultured on PDA plates at a temperature of 25° C. for 7 days until the fungal hyphae covered the entire plate, thereby obtaining PDA plates of the respective fungal strains.

[0068] Use a 1-cm-diameter hole puncher to take three fungal cakes from the PDA plate of each strain, inoculate them into PDB medium, and culture them in a shaking incubator at 25-30°C and 150 rpm for 4 days to obtain fungal activation solution.

[0069] 4. Preparation of bacterial suspension: The fungal mycelium was obtained from the fungal activation solution by centrifugation at a speed of 8000 rpm for 10 min, and the fungal mycelium was resuspended in sterile water to prepare a bacterial suspension of 0.5 g fungal mycelium / 100 mL.

[0070] 5. Inoculation of bacteria: 10 mL of bacterial suspension was inoculated into each flower pot. The inoculation method was as follows: the bacterial suspension was poured from the upper soil of the flower pot to allow the bacterial suspension to penetrate and colonize naturally. That is, 0.05 g of mycelium was inoculated into every 1.5 g of straw. Each group was repeated 4 times and cultured at room temperature.

[0071] 6. Set up the control group (CK): inoculate 10 mL of sterile water in each flower pot.

[0072] 7. Comparison Group: To compare the effectiveness of strains RT199 and RT22 with the corn straw-degrading strain, a comparison group was established. This comparison group used Bacillus cereus W118 to degrade corn straw. Bacillus cereus W118 has a CGMCC accession number of 23973 (see patent CN 115537349 A for details). The strain was activated and expanded according to the method described in CN 115537349 A. The strain was then inoculated and tested on corn straw according to the methods described in CN 115537349 A. This served as the comparison group.

[0073] 8. Determination method: After 30 days, the net bags of the experimental group, control group and comparative group were removed, washed, dried and weighed, and the straw degradation rate was calculated.

[0074] 9. The results of corn straw degradation rate after 30 days of soil bag experiment are as follows: Figure 3 shown.

[0075] Depend on Figure 3 The results showed that the corn straw degradation rate in the CK group was 14.93%. Within 30 days after inoculation with RT199, the corn straw weight loss rate reached 70.53%. Within 30 days after inoculation with RT22, the corn straw weight loss rate was 33.8%. The corn straw weight loss rate in the comparison group inoculated with Bacillus cereus W118 was 35.22%. Compared with the CK group, corn straw degradation in the mesh bags treated with RT199 was significantly higher, with extremely high fragmentation and a very low recovery rate. These results indicate that RT199 is a phenotypically excellent corn straw-degrading strain, with superior degradation performance compared to currently available corn straw-degrading strains, such as Bacillus cereus.

[0076] Example 3

[0077] RT199 Return to Field Application

[0078] To further verify the practical application effects of RT199, a field straw return experiment was conducted in Jiangning District, Nanjing, Jiangsu Province from March 18 to April 18, 2025. The results showed that during the one-month field return experiment, the application of RT199 increased the degradation rate of corn straw compared to in situ soil, facilitating the return of corn straw to the field.

[0079] Here are the steps:

[0080] The methods for bacterial cell activation and bacterial suspension preparation are the same as those in steps 3 and 4 of Example 2.

[0081] Weigh a certain amount of straw and add water to maintain a moisture content of 60%-65%. Evenly mix the RT199 suspension with the straw, ensuring that approximately 5g of fungal mycelium is applied to every 150g of straw (dry weight). The treatment in which RT199 and corn straw were both plowed and returned to the field served as the experimental group, while the control group was treated with an equal volume of sterile water and corn straw.

[0082] Thirty days after returning the soil to the field, soil carbon pool analysis was conducted: the returned soil was air-dried and passed through a 2 mm sieve. Organic carbon with a particle size >53 μm was separated by physical grouping as particulate organic carbon (POC), and organic carbon with a particle size <53 μm was separated as mineral-bound organic carbon (MAOC).

[0083] Comparison of soil POC and MAOC changes between the experimental group and the control group Figure 4 shown.

[0084] Depend on Figure 4 It can be seen that the POC content of the experimental group was 9.39 g / kg, and that of the control group was 7.88 g / kg, which was 19.16% higher than that of the control group; the MAOC content of the experimental group was 18.6 g / kg, and that of the control group was 13.33 g / kg, which was 39.59% higher than that of the control group; and the total soil organic carbon increased by 31.99%.

[0085] Example 4

[0086] Identification and morphological characterization of RT199 strain

[0087] Molecular biological identification of the RT199 strain was performed by ITS sequence analysis: After extracting the genomic DNA of the RT199 fungus, the ITS (Internal Transcribed Spacer) region was amplified using universal primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′, SEQ ID NO. 1) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′, SEQ ID NO. 2).

[0088] The nucleotide sequence of the ITS of the RT199 strain obtained by sequencing is shown in SEQ ID NO.3, specifically:

[0089] >SEQ ID NO.3

[0090] .

[0091] The ITS sequence of the RT199 strain was uploaded to the NCBI database for BLAST comparison to determine the reference strain with high similarity and construct a phylogenetic tree. The phylogenetic tree is shown in Figure 5 A in.

[0092] The hyphae morphology of RT199 strain was characterized by electron microscopy. Figure 5 As shown in B.

[0093] The electron microscopic observation results of corn stalk morphology 30 days after inoculation with RT199 strain in Example 2 are shown in FIG. Figure 6 shown. Figure 6 The two pictures in the figure are the results of observation of corn stalk morphology at different angles. Figure 6 It was further demonstrated that after inoculation with RT199, the surface of corn stalks was obviously wrapped and destroyed by hyphae, and the fragmented stalks further attracted bacterial colonization and utilization.

[0094] Through the above molecular biological identification and morphological observation, it was confirmed that the RT199 strain was Fusarium equisetifolia. Fusarium equisetiOn March 10, 2025, the RT199 strain was deposited in the General Microbiology Center of the China Culture Collection Administration of Microorganisms with the deposit number CGMCC No. 41794.

[0095] In summary, the Fusarium equisetifolia RT199 provided by the present invention can efficiently degrade straw at room temperature, increase soil carbon pool content, and thus promote crop yield. The Fusarium equisetifolia RT199 provided by the present invention is suitable for resource utilization of agricultural waste such as straw and soil improvement, thereby helping to improve the efficiency of agricultural waste resource utilization and enhance the quality of cultivated land.

[0096] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A Fusarium equisetum fungus ( Fusarium equiseti ) RT199, characterized in that The deposit number of the Fusarium equisetifolia RT199 is CGMCC No. 41794.

2. A fermentation agent, characterized in that: The invention comprises the Fusarium equisetifolia RT199 described in claim 1.

3. A method for preparing the fermentation agent according to claim 2, characterized in that: include: The Fusarium equisetifolia RT199 is cultured in a culture medium to obtain a fermentation agent.

4. The preparation method according to claim 3, characterized in that The culture temperature is 25-30° C., and the culture time is 4-7 days.

5. Use of the Fusarium equisetifolia RT199 according to claim 1, the fermentation agent according to claim 2, or the fermentation agent prepared by the preparation method according to claim 3 or 4 in straw degradation; the straw is corn straw.

6. A method for degrading straw, characterized in that: include: inoculating the Fusarium equisetifolia RT199 according to claim 1 into straw for fermentation; The straw is corn straw.

7. The method according to claim 6, characterized in that The fermentation temperature is 25-30° C. and the fermentation time is ≥30 days.

8. The method according to claim 6, characterized in that During inoculation, the mass ratio of the dry weight of the straw to the fungal mycelium of the Fusarium equisetifolia RT199 is 15:1 to 30:

1.

9. The method according to claim 8, characterized in that The preparation method of the fungal mycelium comprises: Cultivating the Fusarium equisetifolia RT199 in a culture medium to obtain a fungus activation solution; Fungal mycelium is separated from the fungus activation liquid.

10. Use of the method according to any one of claims 6 to 9 in improving soil; wherein the soil improvement comprises increasing the soil POC content and / or soil MAOC content.

Images