Steam sterilization device for microalgae culture medium and pressure control system of steam sterilization device

Abstract

The invention discloses a microalgae culture medium steam sterilization device and a pressure control system thereof, and relates to the field of culture medium sterilization, the microalgae culture medium steam sterilization device comprises a steam sterilization tank and a treatment mechanism, the treatment mechanism is used for placing and adjusting the direction of a microalgae culture medium, the top of the steam sterilization tank is provided with a sealing cover through a flange, and the outer wall of the steam sterilization tank is provided with a controller; the treatment mechanism is composed of a placing frame, an adjusting assembly and a clamping assembly, the placing frame comprises two vertical plates and two arc-shaped connecting rods, the clamping assembly is arranged on the adjusting assembly and used for reversely buckling and fixing microalgae culture media with different diameters, and the adjusting assembly is arranged on the vertical plates and used for changing the inclination angle of the microalgae culture media. The sprayed steam is matched to drive the microalgae culture medium to rotate, so that cold spots and hot spots are prevented from being formed. The microalgae culture medium can be inclined and rotated, the rotating culture medium can break laminar flow of steam, the problem of local overheating or insufficient sterilization is avoided, and more uniform temperature distribution is formed.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of culture medium sterilization, and in particular to a steam sterilization device for microalgae culture medium and a pressure control system thereof. Background Art

[0002] With the continuous evolution of bioengineering technology, microorganisms have demonstrated their potential for processing and application in many industrial fields. In the preparation process of microorganisms, culture medium is usually required to provide a suitable environment for survival and reproduction. Therefore, the sterilization process of the culture medium has become a core step to ensure the sterility of the microalgae culture environment.

[0003] A steam-jet fermentation medium heating sterilizer described in the prior art includes a medium fixing device, a control base installed at the bottom of the sterilizer, a protective cover installed at the top of the sterilizer with a sealed connection, an inner liner provided inside the sterilizer, and the medium fixing device is evenly distributed in a circular array on the upper surface of the inner liner. The medium fixing device consists of a clamping bracket, a driving mechanism, and a release mechanism. The clamping bracket is in a "cross" structure and is evenly distributed in a circular array on the upper surface of the inner liner.

[0004] Although the above technology can fix the four sides of the culture medium through the clamping bracket during the steam sterilization of the culture medium, the culture medium will not move even if the inner liner is tilted during the taking and placing process, thereby avoiding the culture medium colliding with the inner liner. However, when the steam flows in the sterilizer, it is difficult to effectively cover each culture medium, especially for the culture medium fixing device distributed in a circular array. It is difficult for the steam to reach the position of each culture medium evenly, resulting in some culture media being in the dead corner of the steam flow, forming cold spots and hot spots, and the sterilization effect is uneven. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to provide a steam sterilization device for microalgae culture medium and a pressure control system thereof to solve the technical problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A steam sterilization device for microalgae culture medium, comprising a steam sterilization tank and an internal processing mechanism for placing and adjusting the direction of the microalgae culture medium, a sealing cover mounted on the top of the steam sterilization tank via a flange, a controller mounted on the outer wall of the steam sterilization tank, an air inlet pipe connected to the bottom of the steam sterilization tank, an annular pipe mounted on the inner bottom of the steam sterilization tank, a plurality of air injection pipes uniformly connected to the upper surface of the annular pipe, one end of the air inlet pipe being connected to the annular pipe, symmetrically fixed to the inner wall of the steam sterilization tank with overlapping blocks, and positioning rods fixed to one side of the upper surface of two overlapping blocks;

[0008] The processing mechanism consists of a placement rack, an adjustment component and a clamping component. The placement rack includes two vertical plates and two arc-shaped connecting rods. The bottom ends of the two vertical plates are in contact with the upper surface of the overlap block. The clamping component is arranged on the adjustment component and is used to fix microalgae culture media of different diameters upside down. The adjustment component is arranged on the vertical plate and is used to change the inclination angle of the microalgae culture media. It also cooperates with the ejected steam to drive the microalgae culture media to rotate to avoid the formation of cold spots and hot spots.

[0009] Specifically, the two ends of the two arc-shaped connecting rods are fixedly connected to the outer wall of one side of the two vertical plates, and the bottom of each vertical plate is provided with a square moving groove and a circular positioning groove, and the positioning groove matches the positioning rod;

[0010] A movable plate is provided inside the movable groove for sliding, and a plurality of fixed grooves are evenly provided on the inner side wall of the movable groove away from the positioning groove. A plurality of fixed rods matching the fixed grooves are fixed on one side wall of the movable plate. A plurality of adjustment grooves are evenly provided on a side of the vertical plate away from the inner wall of the steam sterilization tank, and each of the adjustment grooves is communicated with the movable groove. An inner tooth is provided on a side of the movable plate close to the adjustment groove.

[0011] Specifically, the technical solution of this invention is provided with a pushing groove at the center of the other side of the movable plate, the length and width of the movable plate are both smaller than the length and width of the movable groove, and a push plate is fixed on the other side of the movable plate, the push plate passes through the pushing groove and is slidably connected, and the two arc-shaped connecting rods are connected by a fixed supporting arc plate.

[0012] The specific technical solution of this invention is that a plurality of adjustment components are provided, and each adjustment component includes a U-shaped block, and a meshing gear is fixedly installed at the U-shaped groove of the U-shaped block, and one side tooth surface of the meshing gear passes through the adjustment groove and is meshed with the inner track tooth. Both side walls of the U-shaped end of the U-shaped block are rotatably connected to the adjustment groove wall through an axle rod, and a rotating shaft is rotatably passed through the bottom end of the U-shaped block, and the end of the rotating shaft is fixedly connected to a circular plate.

[0013] Specifically, the rotating shaft is provided with a fan blade, and the side walls of the annular tube are connected to two vertical air outlet pipes, the two air outlet pipes are respectively located on one side of the two vertical plates, and the outer walls of the two air outlet pipes close to the fan blades are connected to horizontal air guide pipes, and the ports of the air guide pipes are matched with the blades of the fan blades;

[0014] An inclined guiding inclined plate is welded to the top of one side of the two overlapping blocks away from the inner wall of the steam sterilization tank.

[0015] Specifically, the present technical solution comprises two L-shaped clamping seats, both of which are located on a circular plate arranged in the adjustment assembly, and the inner walls of the grooves of the circular plate are provided with sliding grooves, and sliders are slidably installed in the two sliding grooves, and a movable base is fixed between the two sliders, one of the L-shaped clamping seats is fixedly connected to the top of the movable base, and the other L-shaped clamping seat is fixedly connected to one end of the upper surface of the circular plate away from the inner wall of the steam sterilization tank.

[0016] The present technical solution is specific, the L end of the L-shaped clamping seat is provided with a movable groove, the top of the L end of the L-shaped clamping seat is provided with a sliding movable splint, the bottom end of the movable splint is fixed with a movable plate, the movable plate matches the movable groove and is slidably connected to the groove wall, the L-shaped clamping seat is rotated away from the movable splint and is provided with a rotary handle, the movable plate is fixed with a square sleeve on the outer wall facing the movable groove, the groove wall of the movable groove is provided with a receiving square groove matching the square sleeve, a threaded rod is installed inside the square sleeve, one end of the threaded rod passes through the receiving square groove and extends to the outside of the L-shaped clamping seat, and the end of the threaded rod is fixed with a rotary handle.

[0017] Specifically, the top center of the sealing cover is connected to a vent pipe, the port of the vent pipe is fixed with a connecting pipe through a flange, the connecting pipe is fixedly connected to the outer wall of the steam sterilization tank through a bracket, and a safety valve is installed at the other end of the connecting pipe.

[0018] According to the above technical solution, a pressure control system for a steam sterilization device for microalgae culture medium is also provided, wherein a pressure sensor and a temperature sensor are installed through the top wall of the sealing cover, and a solenoid valve is installed inside the port of the air inlet pipe away from the annular pipe;

[0019] The controller is internally provided with a microprocessor module, a wireless module and a communication module. The controller is electrically connected to the pressure sensor, temperature sensor, solenoid valve and safety valve respectively through the wireless module. The microprocessor module is responsible for data processing and command output to ensure real-time monitoring and adjustment, and to ensure that the sterilization process is accurate and efficient. The communication module realizes information interaction with each sensor and actuator, analyzes sensor data in real time through algorithm optimization, automatically adjusts the opening of the solenoid valve and safety valve, accurately controls the steam flow, and ensures temperature and pressure stability.

[0020] In summary, the present invention has the following major beneficial effects: the placement rack and adjustment assembly enable the microalgae culture medium to be tilted and rotated. The rotating culture medium can disrupt the laminar flow of steam, avoiding local overheating or insufficient sterilization, and creating a more uniform temperature distribution. This dynamic adjustment can effectively avoid dead corners in steam flow, ensuring that each culture medium is fully exposed to steam, thereby improving the uniformity of the sterilization effect.

[0021] Through the synergistic effect of the fan blades and the corresponding air ducts, the steam from the air ducts impacts the fan blades. The faster the steam input rate, the greater the impact force and the higher the fan blade speed, thus achieving evenly distributed steam. This mechanism further improves the efficiency of the sterilization process, ensures the uniformity of heating of the microalgae culture medium during the sterilization process, and effectively avoids the problem of sterilization failure caused by temperature fluctuations.

[0022] The design of the clamping assembly makes the installation and removal of the culture medium simple and quick. The combination of the L-shaped clamping seat and the movable clamping plate can adapt to culture media of different diameters. Quick fixation or release can be achieved by adjusting the rotary handle, which improves the convenience and versatility of operation and ensures the stability of the culture medium during the sterilization process. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the overall positive axis structure of the device of the present invention;

[0024] Figure 2 This is a schematic diagram of the cross-section of the steam sterilizer of the present invention;

[0025] Figure 3 This is a schematic structural diagram of the processing mechanism of the present invention on the positive axis side;

[0026] Figure 4 This is a schematic diagram of the oblique-axis structural side of the processing mechanism of the present invention;

[0027] Figure 5 For the present invention Figure 4 A partial enlarged view of the middle part;

[0028] Figure 6 This is a schematic diagram of the structure of the placement rack and the bridge block of the present invention when viewed from the bottom;

[0029] Figure 7 It is a schematic side view of the vertical plate structure of the present invention;

[0030] Figure 8 This is a schematic diagram of the connection structure between the adjustment component and the movable plate of the present invention;

[0031] Figure 9 For the present invention Figure 8 Schematic diagram of the top view structure;

[0032] Figure 10 For the present invention Figure 8 A partial enlarged view of point B in the middle;

[0033] Figure 11 This is a schematic side view of the cross-sectional structure of the L-shaped clamping seat of the present invention;

[0034] Figure 12 It is a framework diagram of the pressure control system of the present invention.

[0035] Description of the drawings: 1. Steam sterilizer; 101. Sealing cover; 102. Air release pipe; 103. Connecting pipe; 1031. Safety valve; 2. Air inlet pipe; 201. Ring pipe; 2011. Air outlet pipe; 202. Air guide pipe; 3. Controller; 4. Overlap block; 401. Positioning rod; 402. Guide inclined plate; 5. Processing mechanism; 6. Placement rack; 601. Vertical plate; 6011. Positioning slot; 602. Moving slot; 6021. Fixed slot; 603. Adjusting slot; 604. Pushing slot; 605. Moving plate; 6051. Inner gear; 6052. Fixed Rod; 6053, push plate; 606, arc-shaped connecting rod; 6061, supporting arc plate; 7, adjustment component; 701, U-shaped block; 702, meshing gear; 703, shaft; 704, rotating shaft; 7041, fan blade; 705, circular plate; 7051, slide; 8, clamping component; 801, L-shaped clamping seat; 802, movable groove; 8021, storage square groove; 803, movable splint; 8031, movable plate; 804, square sleeve; 805, threaded rod; 806, rotary handle; 807, movable base; 8071, slider; 9, microalgae culture medium. DETAILED DESCRIPTION

[0036] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0037] The following describes an embodiment of the present invention based on its overall structure.

[0038] In this embodiment, please refer to Figures 1-6 As shown, a steam sterilization device for microalgae culture medium comprises a steam sterilization tank 1 and a processing mechanism 5 for placing and adjusting the direction of a microalgae culture medium 9 inside the steam sterilization tank 1, a sealing cover 101 is installed on the top of the steam sterilization tank 1 through a flange, a controller 3 is installed on the outer wall of the steam sterilization tank 1, an air release pipe 102 is connected at the center of the top of the sealing cover 101, a connecting pipe 103 is fixed to the end of the air release pipe 102 through a flange, the connecting pipe 103 is fixedly connected to the outer wall of the steam sterilization tank 1 through a bracket, a safety valve 1031 is installed on the other end of the connecting pipe 103, an air inlet pipe 2 is connected to the bottom of the steam sterilization tank 1, an annular pipe 201 is installed on the inner bottom of the steam sterilization tank 1, a plurality of air injection pipes are evenly connected on the upper surface of the annular pipe 201, one end of the air inlet pipe 2 is connected to the annular pipe 201, wherein the other end of the air inlet pipe 2 is connected to an external steam source, and overlapping blocks 4 are symmetrically fixed on the inner wall of the steam sterilization tank 1, and positioning rods 401 are fixed on one side of the upper surface of the two overlapping blocks 4;

[0039] The processing mechanism 5 consists of a placement rack 6, an adjustment component 7 and a clamping component 8. The placement rack 6 includes two vertical plates 601 and two arc-shaped connecting rods 606. The bottom ends of the two vertical plates 601 are in contact with the upper surface of the overlap block 4. The clamping component 8 is arranged on the adjustment component 7 and is used to fix microalgae culture media 9 of different diameters upside down. The adjustment component 7 is arranged on the vertical plate 601 and is used to change the inclination angle of the microalgae culture media 9. It also cooperates with the ejected steam to drive the microalgae culture media 9 to rotate to avoid the formation of cold spots and hot spots.

[0040] When steam sterilizing the microalgae culture medium 9, the operator fixes the microalgae culture medium 9 to be sterilized by the clamping assembly 8, then adjusts the adjustment assembly 7 so that the microalgae culture medium 9 reaches a predetermined tilt angle, places the two vertical plates 601 into the steam sterilization tank 1, and inserts the positioning rod 401 into the positioning groove 6011 to complete the positioning and fixing of the vertical plates 601. Subsequently, the sealing cover 101 is placed on the top of the steam sterilization tank 1 and locked by the flange. The air release pipe 102 is then sealed and connected to the connecting pipe 103 by the flange. The steam source is then started, and the controller 3 activates the solenoid valve in the air inlet pipe 2, so that steam enters the annular pipe 201 and is ejected through multiple air injection pipes. At the same time, part of the steam is also ejected from the air outlet pipe 2011 and the air guide pipe 202 to form a steam environment, ensuring that the microalgae culture medium 9 is sterilized in a uniform steam environment.

[0041] After the steam is ejected from the air duct 202, it drives the fan blades 7041 in the regulating assembly 7 to rotate, causing the originally tilted microalgae culture medium 9 to slowly rotate under the drive of the fan blades 7041, thereby evenly heating it. When the temperature or pressure inside the steam sterilizer 1 reaches the set value, the controller 3 automatically adjusts the safety valve 1031 to release excess steam, maintaining a stable environment and ensuring that the microalgae culture medium 9 is fully sterilized.

[0042] After the sterilization is completed, the operator turns off the steam source, waits until the temperature inside the tank drops to a safe range, opens the sealing cover 101, takes out the microalgae culture medium 9, checks the sterilization effect, and after confirming that it is correct, restores the equipment to its initial state, records the operation data, and completes the entire sterilization process;

[0043] Therefore, the present invention can tilt and rotate the microalgae culture medium 9 through the placement rack 6 and the adjustment component 7. The rotating culture medium can break the laminar flow of steam, avoid local overheating or insufficient sterilization, and form a more uniform temperature distribution. This dynamic adjustment can effectively avoid dead corners of steam flow, ensure that each culture medium can fully contact the steam, and improve the uniformity of the sterilization effect. Through the synergistic effect of the fan blades 7041 and the corresponding air ducts 202, the rotation speed of the fan blades 7041 can be automatically adjusted according to the steam input rate to achieve balanced distribution of steam. This mechanism further improves the efficiency of the sterilization process.

[0044] See also Figure 8 、 Figure 10 and Figure 11 As shown, the clamping assembly 8 includes two L-shaped clamping seats 801, and the two L-shaped clamping seats 801 are both located on the circular plate 705 provided in the adjustment assembly 7. The inner wall of the notch of the circular plate 705 is provided with a slide groove 7051, and a slider 8071 is slidably installed in the two slide grooves 7051. A movable base 807 is fixed between the two sliders 8071. One L-shaped clamping seat 801 is fixedly connected to the top of the movable base 807, and the other L-shaped clamping seat 801 is fixedly connected to the end of the upper surface of the circular plate 705 away from the inner wall of the steam sterilization tank 1. The L end of the L-shaped clamping seat 801 is provided with a movable groove 802, and the top of the L end of the L-shaped clamping seat 801 is provided with a sliding The movable splint 803 has a movable plate 8031 fixed to its bottom end, which matches the movable groove 802 and is slidably connected to the groove wall. A rotary handle 806 is provided on the side of the L-shaped clamping seat 801 that is away from the movable splint 803. A square sleeve 804 is fixed to the outer wall of the movable plate 8031 facing the movable groove 802. A receiving square groove 8021 matching the square sleeve 804 is provided on the groove wall of the movable groove 802. A threaded rod 805 is installed inside the square sleeve 804. One end of the threaded rod 805 passes through the receiving square groove 8021 and extends out of the outside of the L-shaped clamping seat 801, and a rotary handle 806 is fixed to the end of the threaded rod 805.

[0045] When installing and placing the microalgae culture medium 9, the operator inverts the microalgae culture medium 9 to be sterilized between the two L-shaped clamping seats 801 and pushes the movable base 807 to move the slider 8071 along the slide groove 7051, driving the movable L-shaped clamping seat 801 to move closer to the fixed L-shaped clamping seat 801 until the L ends of the two L-shaped clamping seats 801 are close to the outer wall of the culture medium port. At this time, the handle 806 is manually rotated to drive the threaded rod 805 to rotate. The square sleeve 804 follows the rotation of the threaded rod 805 under the restriction of the receiving square groove 8021 to move horizontally Sliding, the square sleeve 804 pushes the movable plate 8031 to move along the movable groove 802, so that the movable clamping plate 803 moves with the movable plate 8031 and approaches the inner wall of the port of the microalgae culture medium 9 until it is tightly attached to complete the clamping operation. The L end of the L-shaped clamping seat 801 and the end face of the movable clamping plate 803 are both provided with anti-slip grooves to ensure firm clamping. At the same time, multiple holes can be opened to ensure uniform steam penetration in the clamping part, further improving the sterilization effect, and being able to adapt to microalgae culture media 9 of different diameters, thereby improving operational convenience and versatility.

[0046] See also Figure 3-Figure 9 As shown, the two ends of the two arc-shaped connecting rods 606 are fixedly connected to the outer wall of one side of the two vertical plates 601 respectively. The bottom of each vertical plate 601 is provided with a square moving groove 602 and a circular positioning groove 6011, and the positioning groove 6011 matches the positioning rod 401;

[0047] A movable plate 605 is provided for sliding inside the movable groove 602, and a plurality of fixed grooves 6021 are evenly provided on the inner side wall of the movable groove 602 away from the positioning groove 6011, and a plurality of fixed rods 6052 matching the fixed groove 6021 are fixed to one side wall of the movable plate 605. A plurality of adjustment grooves 603 are evenly provided on the side of the vertical plate 601 away from the inner wall of the steam sterilization tank 1, and each adjustment groove 603 is communicated with the movable groove 602. An inner tooth 6051 is provided on the side of the movable plate 605 close to the adjustment groove 603, and a pushing groove 604 is provided in the center of the other side of the movable plate 605. The length and width of the movable plate 605 are both smaller than those of the movable groove 602. A push plate 6053 is fixed on the other side of the movable plate 605, and the push plate 6053 passes through the pushing groove 604 and is slidably connected. The two arc-shaped connecting rods 606 are connected by a fixed supporting arc plate 6061;

[0048] There are multiple adjustment components 7 (the same number as the adjustment slots 603), each adjustment component 7 includes a U-shaped block 701, a meshing gear 702 is fixedly installed at the U-shaped slot of the U-shaped block 701, one side of the tooth surface of the meshing gear 702 passes through the adjustment slot 603 and is meshed with the inner gear 6051, and both side walls of the U-shaped end of the U-shaped block 701 are rotatably connected to the groove wall of the adjustment slot 603 through the shaft 703, and the bottom end of the U-shaped block 701 is rotatably penetrated by a rotating shaft 704, and the end of the rotating shaft 704 is fixedly connected It is connected to a circular plate 705, and a fan blade 7041 is fixedly sleeved on the rotating shaft 704. The side walls of the annular tube 201 are connected to two vertical air outlet pipes 2011, and the two air outlet pipes 2011 are respectively located on one side of the two vertical plates 601. The outer walls of the two air outlet pipes 2011 close to the fan blade 7041 are connected to a horizontal air guide pipe 202, and the port of the air guide pipe 202 cooperates with the blade of the fan blade 7041. An inclined guide ramp 402 is welded on the top of the two overlapping blocks 4 away from the inner wall of the steam sterilization tank 1.

[0049] After the microalgae culture medium 9 is clamped and fixed, the operator pushes the push plate 6053 toward one side of the push groove 604, so that the push plate 6053 drives the movable plate 605 to move in the movable groove 602, and the inner track teeth 6051 also slide along the tooth surface of the meshing gear 702. At this time, the movable plate 605 drives the fixed rod 6052 to move out of the fixed groove 6021, thereby unlocking the movable plate 605.

[0050] At this time, the push plate 6053 can be pushed upward along the push slot 604, causing the movable plate 605 to drive the fixed rod 6052 to move. At the same time, the inner gear 6051 drives the meshing gear 702 to rotate. The rotating meshing gear 702 drives the U-shaped block 701 to change its angle within the adjustment slot 603. When the U-shaped block 701 changes its angle, it drives the circular plate 705 to change its angle via the rotating shaft 704. The circular plate 705 drives the fixed microalgae culture medium 9 to adjust its angle via the L-shaped clamping seat 801.

[0051] Then, after the steam enters the steam sterilizer 1, part of the steam will be discharged from the air outlet pipe 2011 and guided to the fan blades 7041 through the air guide pipe 202, driving the fan blades 7041 to rotate. The rotating fan blades 7041 will drive the circular plate 705 to rotate through the rotating shaft 704, thereby controlling the microalgae culture medium 9 to rotate. The guiding inclined plate 402 can guide the flow direction of the steam, reduce the frontal impact between the steam from below and the fan blades 7041, and the rotating culture medium can change the flow direction of the steam, break the laminar flow of the steam, and make the steam more evenly distributed, avoiding local overheating or insufficient sterilization problems, forming a more uniform temperature distribution, and realizing all-round sterilization of the microalgae culture medium 9.

[0052] See also Figure 1 and Figure 12 As shown, according to the above embodiment, a pressure control system of a steam sterilization device for microalgae culture medium is also provided. A pressure sensor and a temperature sensor are installed through the top wall of the sealing cover 101, and a solenoid valve is installed inside the port of the air inlet pipe 2 away from the annular pipe 201;

[0053] The controller 3 is internally provided with a microprocessor module (using an ARM processor), a wireless module and a communication module (using ZigBee wireless communication technology). The controller 3 is electrically connected to the pressure sensor, temperature sensor, solenoid valve and safety valve 1031 through the wireless module. The microprocessor module is responsible for data processing and command output to ensure real-time monitoring and adjustment, and to ensure that the sterilization process is accurate and efficient. It can ensure that during the sterilization process, the pressure and temperature can be kept within the set safety range, thereby improving the sterilization efficiency and safety performance. The communication module realizes information interaction with various sensors and actuators, analyzes sensor data in real time through algorithm optimization, automatically adjusts the opening of the solenoid valve and safety valve 1031, accurately controls the steam flow, and ensures temperature and pressure stability.

[0054] According to the actual parameters fed back by the pressure sensor and the temperature sensor, the opening degree of the solenoid valve and the safety valve 1031 are intelligently controlled to ensure that the pressure and temperature inside the device meet the set values. When large pressure or temperature fluctuations are detected, the pressure sensor and the temperature sensor will generate a signal feedback to the controller 3. After receiving the signal, the controller 3 will adjust the switching state of the solenoid valve and the safety valve 1031 to quickly restore the interior of the steam sterilization tank 1 to the set value.

[0055] The working principle of the present invention is:

[0056] When steam sterilizing the microalgae culture medium 9, the operator inverts the microalgae culture medium 9 to be sterilized between the two L-shaped clamping seats 801 and pushes the movable base 807 to move the slider 8071 along the slide groove 7051, driving the movable L-shaped clamping seat 801 to move closer to the fixed L-shaped clamping seat 801 until the L ends of the two L-shaped clamping seats 801 are in close contact with the outer wall of the culture medium port. At this time, the handle 806 is manually rotated to drive the threaded rod 805 to rotate. The square sleeve 804 slides horizontally following the rotation of the threaded rod 805 under the restriction of the receiving square groove 8021. The square sleeve 804 pushes the movable plate 8031 to move along the movable groove 802, so that the movable clamping plate 803 follows the movable plate 8031 to move closer to the inner wall of the port of the microalgae culture medium 9 until it is in close contact with the inner wall, completing the clamping operation.

[0057] After the microalgae culture medium 9 is clamped and fixed, the operator pushes the push plate 6053 toward one side of the push groove 604, so that the push plate 6053 drives the movable plate 605 to move in the movable groove 602, and the inner track teeth 6051 also slide along the tooth surface of the meshing gear 702. At this time, the movable plate 605 drives the fixed rod 6052 to move out of the fixed groove 6021, thereby unlocking the movable plate 605.

[0058] At this time, the push plate 6053 can be pushed upward along the push slot 604, causing the movable plate 605 to drive the fixed rod 6052 to move. At the same time, the inner gear 6051 drives the meshing gear 702 to rotate. The rotating meshing gear 702 drives the U-shaped block 701 to change its angle within the adjustment slot 603. When the U-shaped block 701 changes its angle, it drives the circular plate 705 to change its angle via the rotating shaft 704. The circular plate 705 drives the fixed microalgae culture medium 9 to adjust its angle via the L-shaped clamping seat 801.

[0059] Place the two vertical plates 601 into the steam sterilizer 1 so that the positioning rods 401 are inserted into the positioning grooves 6011 to complete the positioning and fixing of the vertical plates 601. Then, cover the top of the steam sterilizer 1 with the sealing cover 101 and lock it with the flange. Then, seal the air release pipe 102 with the connecting pipe 103 through the flange. Then, start the steam source and activate the solenoid valve in the air inlet pipe 2 by the controller 3. This allows steam to enter the annular pipe 201 and be ejected through multiple air injection pipes. At the same time, some steam is also ejected from the air outlet pipe 2011 and the air guide pipe 202 to form a steam environment, ensuring that the microalgae culture medium 9 is sterilized in a uniform steam environment.

[0060] Part of the steam will be discharged from the outlet pipe 2011 and guided to the fan blades 7041 through the air guide pipe 202, driving the fan blades 7041 to rotate. The rotating fan blades 7041 will drive the circular plate 705 to rotate via the rotating shaft 704, thereby controlling the microalgae culture medium 9 to rotate as well, so that the culture medium is evenly heated in the steam. When large pressure or temperature fluctuations are detected, the pressure sensor and the temperature sensor will generate a signal feedback to the controller 3. After receiving the signal, the controller 3 will adjust the switch status of the solenoid valve and the safety valve 1031 to maintain a stable environment inside the steam sterilization tank 1 and ensure that the microalgae culture medium 9 is fully sterilized.

[0061] After the sterilization is completed, the operator turns off the steam source, waits until the temperature in the tank drops to a safe range, opens the sealing cover 101, takes out the microalgae culture medium 9, checks the sterilization effect, and after confirming that it is correct, restores the equipment to its initial state, records the operation data, and completes the entire sterilization process.

[0062] Although an embodiment of the present invention has been shown and described, this specific embodiment is merely an explanation of the present invention and is not a limitation of the invention. The specific features, structures, materials or characteristics described may be combined in an appropriate manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions and variations to the embodiment without creative contribution as needed without departing from the principles and purpose of the present invention. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A steam sterilization device for microalgae culture medium, comprising a steam sterilization tank (1) and a processing mechanism (5) for placing and adjusting the direction of microalgae culture medium (9) inside, a sealing cover (101) being installed on the top of the steam sterilization tank (1) through a flange, and a controller (3) being installed on the outer wall of the steam sterilization tank (1), characterized in that: The bottom of the steam sterilization tank (1) is connected to an air inlet pipe (2), the inner bottom of the steam sterilization tank (1) is installed with an annular pipe (201), the upper surface of the annular pipe (201) is evenly connected with a plurality of air injection pipes, one end of the air inlet pipe (2) is connected to the annular pipe (201), the inner wall of the steam sterilization tank (1) is symmetrically fixed with lap blocks (4), and one side of the upper surface of two lap blocks (4) is fixed with a positioning rod (401); The processing mechanism (5) is composed of a placement rack (6), an adjustment component (7) and a clamping component (8). The placement rack (6) includes two vertical plates (601) and two arc-shaped connecting rods (606). The bottom ends of the two vertical plates (601) are in contact with the upper surface of the overlap block (4). The clamping component (8) is arranged on the adjustment component (7) and is used to fix microalgae culture media (9) of different diameters upside down. The adjustment component (7) is arranged on the vertical plate (601) and is used to change the inclination angle of the microalgae culture media (9). It also drives the microalgae culture media (9) to rotate in conjunction with the ejected steam to avoid the formation of cold spots and hot spots.

2. The steam sterilization device for microalgae culture medium according to claim 1, characterized in that: The two ends of the two arc-shaped connecting rods (606) are respectively fixedly connected to the outer wall of one side of the two vertical plates (601), and the bottom of each vertical plate (601) is provided with a square moving groove (602) and a circular positioning groove (6011), and the positioning groove (6011) matches the positioning rod (401); A movable plate (605) is slidably provided inside the movable groove (602), and a plurality of fixed grooves (6021) are evenly provided on the inner side wall of the movable groove (602) away from the positioning groove (6011). A plurality of fixed rods (6052) matching the fixed grooves (6021) are fixed on one side wall of the movable plate (605). A plurality of adjustment grooves (603) are evenly provided on the side of the vertical plate (601) away from the inner wall of the steam sterilization tank (1), and each of the adjustment grooves (603) is communicated with the movable groove (602). An inner tooth (6051) is provided on the side of the movable plate (605) close to the adjustment groove (603).

3. The steam sterilization device for microalgae culture medium according to claim 2, characterized in that: A pushing groove (604) is provided at the center of the other side of the movable plate (605). The length and width of the movable plate (605) are both smaller than the length and width of the movable groove (602). A push plate (6053) is fixed to the other side of the movable plate (605). The push plate (6053) passes through the pushing groove (604) and is slidably connected. The two arc-shaped connecting rods (606) are connected via a fixed supporting arc plate (6061).

4. The steam sterilization device for microalgae culture medium according to claim 2, characterized in that: A plurality of adjustment assemblies (7) are provided, each of the adjustment assemblies (7) comprising a U-shaped block (701), a meshing gear (702) being fixedly mounted at the U-shaped groove of the U-shaped block (701), a tooth surface on one side of the meshing gear (702) passing through the adjustment groove (603) and meshingly connected with the inner track gear (6051), both side walls of the U-shaped end of the U-shaped block (701) being rotatably connected to the groove wall of the adjustment groove (603) via a shaft (703), a rotating shaft (704) being rotatably passed through the bottom end of the U-shaped block (701), and a circular plate (705) being fixedly connected to the end of the rotating shaft (704).

5. The steam sterilization device for microalgae culture medium according to claim 4, characterized in that: The rotating shaft (704) is fixedly sleeved with a fan blade (7041); the side walls of the annular tube (201) are connected to two vertical air outlet pipes (2011); the two air outlet pipes (2011) are respectively located on one side of the two vertical plates (601); the outer walls of the two air outlet pipes (2011) close to the fan blade (7041) are connected to a horizontal air guide pipe (202); the ports of the air guide pipe (202) are matched with the blades of the fan blade (7041); An inclined guiding inclined plate (402) is welded to the top of one side of the two overlapping blocks (4) away from the inner wall of the steam sterilization tank (1).

6. The steam sterilization device for microalgae culture medium according to claim 1, characterized in that: The clamping assembly (8) includes two L-shaped clamping seats (801), both of which are located on a circular plate (705) provided in the adjustment assembly (7), and the inner wall of the notch of the circular plate (705) is provided with a slide groove (7051), and a slider (8071) is slidably installed in the two slide grooves (7051), and a movable base (807) is fixed between the two sliders (8071), one of the L-shaped clamping seats (801) is fixedly connected to the top of the movable base (807), and the other L-shaped clamping seat (801) is fixedly connected to the end of the upper surface of the circular plate (705) away from the inner wall of the steam sterilization tank (1).

7. The steam sterilization device for microalgae culture medium according to claim 6, characterized in that: The L end of the L-shaped clamping seat (801) is provided with a movable groove (802), the top of the L end of the L-shaped clamping seat (801) is provided with a sliding movable clamping plate (803), the bottom end of the movable clamping plate (803) is fixed with a movable plate (8031), the movable plate (8031) matches the movable groove (802) and is slidably connected to the groove wall, and the side of the L-shaped clamping seat (801) away from the movable clamping plate (803) is provided with a rotary handle (806), and the movable plate A square sleeve (804) is fixed on the outer wall of the movable groove (802) (8031), and a receiving square groove (8021) matching the square sleeve (804) is opened on the groove wall of the movable groove (802). A threaded rod (805) is installed inside the square sleeve (804), and one end of the threaded rod (805) passes through the receiving square groove (8021) and extends out of the L-shaped clamping seat (801), and a rotary handle (806) is fixed to the end of the threaded rod (805).

8. The steam sterilization device for microalgae culture medium according to claim 1, characterized in that: The top center of the sealing cover (101) is connected to a vent pipe (102), the end of the vent pipe (102) is fixed with a connecting pipe (103) through a flange, the connecting pipe (103) is fixedly connected to the outer wall of the steam sterilization tank (1) through a bracket, and the other end of the connecting pipe (103) is installed with a safety valve (1031).

9. The pressure control system of a steam sterilization device for microalgae culture medium according to any one of claims 1 to 8, characterized in that: A pressure sensor and a temperature sensor are installed through the top wall of the sealing cover (101), and a solenoid valve is installed inside the port of the air inlet pipe (2) away from the annular pipe (201); The controller (3) is internally provided with a microprocessor module, a wireless module and a communication module. The controller (3) is electrically connected to the pressure sensor, the temperature sensor, the solenoid valve and the safety valve (1031) respectively via the wireless module.

10. The pressure control system of a steam sterilization device for microalgae culture medium according to claim 9, characterized in that: The microprocessor module is responsible for data processing and command output, ensuring real-time monitoring and adjustment, and ensuring that the sterilization process is accurate and efficient. The communication module realizes information interaction with various sensors and actuators, analyzes sensor data in real time through algorithm optimization, automatically adjusts the opening of the solenoid valve and the safety valve (1031), accurately controls the steam flow, and ensures temperature and pressure stability.

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