Deep hypothermia access equipment and access method for biological sample test tube box

Abstract

The invention provides deep hypothermia access equipment and access method for a biological sample test tube box, and belongs to the technical field of test tube box access. Comprising a tank body, a mounting plate is fixedly connected to the upper surface of the tank body, an inlet and outlet communicated with the tank body is formed in the mounting plate, a plurality of cryopreservation frames are hung on the periphery of the inlet and outlet, and a foam cover plate is mounted on the periphery of the inlet and outlet; a lifting clamping jaw assembly, a guide assembly, a cover plate placing assembly and an inserting, taking and transferring assembly are installed on the upper surface of the rotary disc. A plurality of cryopreservation racks in a tank body can be automatically stored and extracted, meanwhile, photographing is conducted through a camera, the cryopreservation racks are stored in a management system for unified management, the storage position can be accurately recognized, the whole process is controlled through accurate transmission, it is ensured that biological sample test tube boxes are accurately positioned and rapidly stored and taken, and the working efficiency is improved. The influence of human factors on the temperature in the tank body and the sample quality is reduced, and the activity and integrity of the sample are prevented from being damaged.

Description

Technical Field

[0001] The present invention belongs to the technical field of test tube box storage and access, and in particular relates to a deep-cold storage and access device and a storage and access method for a biological sample test tube box. Background Art

[0002] As the cornerstone of life science research, the storage quality and security of biological samples are directly related to the reliability and effectiveness of scientific research results. In the field of life sciences, from basic research to clinical applications, biological samples play a vital role. Whether it is the exploration of disease mechanisms, the screening of drug development, or the implementation of personalized medicine, it is inseparable from the support of high-quality biological samples. The number of samples stored in global biobanks has reached billions and is still growing at a rate of 10%-15% per year. These samples contain rich life information and provide key clues for scientists to unlock the mysteries of life.

[0003] Traditional biological sample storage methods, such as manually operated liquid nitrogen tank storage, have many limitations. Manual operation is not only inefficient and difficult to meet the needs of large-scale biological sample storage and management, but also prone to human errors. In addition, it is difficult to ensure the stability of the low-temperature environment during sample storage and retrieval, which can easily lead to damage to the activity and integrity of the samples. Summary of the Invention

[0004] (1) Technical problems to be solved

[0005] In response to the shortcomings of the existing technology, the purpose of the present invention is to provide a deep-cryogenic storage and access device and access method for a biological sample test tube box, so as to solve the problem that the existing biological sample storage in liquid nitrogen tanks with manual operation has many limitations and is difficult to meet the needs of large-scale biological sample storage and management. It is difficult to ensure the stability of the low-temperature environment during the sample storage and access process, which easily leads to damage to the activity and integrity of the sample.

[0006] (2) Technical solution

[0007] In order to solve the above technical problems, the present invention provides a cryogenic storage and retrieval device for a biological sample test tube box, comprising a tank body, a mounting plate fixedly connected to the upper surface of the tank body, an inlet and outlet communicated with the tank body, a plurality of freezing racks hung around the inlet and outlet and installed with a foam cover plate, a turntable mounted on the upper surface of the mounting plate, a lifting claw assembly, a guide assembly, a cover plate placement assembly, and an insertion and transfer assembly mounted on the upper surface of the turntable, a test tube box entry and exit assembly mounted on the front lower surface of the mounting plate, and a photographing lifting assembly mounted on the entry and exit path of the test tube box entry and exit assembly;

[0008] The turntable is used to drive the lifting clamp assembly, guide assembly, cover placement assembly and insertion and transfer assembly to rotate; the test tube box in and out assembly is used to move the placed test tube box into the photographing lifting assembly; the lifting clamp assembly is used to transfer the foam cover and the freezing rack in the tank body; the insertion and transfer assembly is used to transfer the test tube box between the photographing lifting assembly and the freezing rack.

[0009] Preferably, the freezing rack includes an outer frame, an opening is opened on one side of the outer frame, multiple partitions are fixedly connected to the inner walls on both sides of the outer frame to form multiple storage cavities, the top of the outer frame is fixedly connected to a hanging rod, the edge of the inlet and outlet is fixedly connected to a circle of bosses, and the top of the hanging rod is L-shaped and located on the boss.

[0010] Preferably, the lower surface of the turntable is rotatably connected to the mounting plate through a bearing, the outer surface of the turntable is fixedly connected to a synchronous ring gear, a drive motor is installed on the mounting plate, the output end of the drive motor is meshed with the synchronous ring gear through a gear, and the turntable is concentrically arranged with the inlet and outlet.

[0011] Preferably, the lifting clamp assembly includes a base plate fixedly connected to the upper surface of the turntable, a column is slidably provided on the upper surface of the base plate, a first motor is installed on the right side of the column, the output end of the first motor is connected to a horizontal screw rod through a belt drive, the screw rod is threadedly connected to the bottom end of the column, a second motor is installed on the top of the column and a second movable plate is slidably provided, the output end of the second motor is connected to a vertical screw rod through a belt drive, the screw rod is threadedly connected to the second movable plate, an electric clamp is installed on the second movable plate, and two clamping claws are installed on the output end of the electric clamp.

[0012] Preferably, the guide assembly includes a guide frame slidably connected to the left side of the column and a third motor installed at the top of the column. The output end of the third motor is fixedly connected to a vertical screw rod, which is threadedly connected to the guide frame. A guide hole is opened in the middle of the guide frame, and rollers are installed at the four corners of the guide hole.

[0013] Preferably, the cover plate placement assembly includes a fixing seat fixedly connected to the upper surface of the turntable, a fourth motor is installed on the fixing seat, the output end of the fourth motor is fixedly connected to a placement plate, the upper surface of the placement plate is provided with a placement groove corresponding to the foam cover plate, and the upper surface of the foam cover plate is provided with two clamping grooves.

[0014] Preferably, the test tube box in-and-out assembly includes a driving member and a plurality of vertical rods fixedly connected to the front side of the lower surface of the mounting plate, the bottom ends of the vertical rods are fixedly connected to the in-and-out plate, a tray is slidably mounted on the upper surface of the in-and-out plate, the driving member is used to drive the tray to move along the in-and-out plate, and avoidance grooves are provided at the four corners of the tray.

[0015] Preferably, the photo-taking lifting assembly includes a driving member and a rectangular hole provided on the front side of the mounting plate, two vertical rods are fixedly connected to the inner walls on both the front and rear sides of the rectangular hole, a base is provided on the sliding sleeve of the outer surface of the vertical rod, the driving member is used to drive the base to slide up and down along the vertical rod, a camera is installed on the base, a through hole corresponding to the camera is provided in the middle of the inlet and outlet plate, the four corners of the base are fixedly connected to columns, the top ends of the columns pass through the rectangular holes and are fixedly connected to the top plate, and the adjacent sides of the outer surfaces of the four columns are fixedly connected to support blocks corresponding to the avoidance grooves.

[0016] Preferably, the plug-in transfer assembly includes a lower fixed plate fixedly connected to the upper surface of the turntable, the upper surface of the lower fixed plate is rotatably connected to the upper fixed plate through a gear plate, the upper surface of the upper fixed plate is slidably connected to a movable rod, one end of the movable rod is fixedly connected to the plug-in plate, and a long groove is provided on the lower surface of the other end of the movable rod, a fifth motor is fixedly connected to the right side of the upper surface of the lower fixed plate, and a sixth motor is fixedly connected to the right side of the upper surface of the upper fixed plate, the output end of the fifth motor is connected to the gear plate through a belt transmission, the sixth motor is connected to the rotating shaft through a belt transmission, the bottom end of the rotating shaft is fixedly connected to a rocker arm, and the other end of the rocker arm is fixedly connected to a bearing and is located inside the long groove.

[0017] Preferably, a method for deep cryogenic storage and access of a biological sample test tube box comprises the following steps:

[0018] Step 1: Move the support block on the camera lift assembly to the bottom and wait. Then move the tray of the test tube box in and out assembly to the left, and then place the test tube box on the tray.

[0019] Step 2: After the tray senses that a test tube box has been placed in the tray, it moves to the top of the support block, then moves the support block upward to the avoidance groove, and moves the test tube box on the tray to the upper side of the mounting plate;

[0020] Step 3: The tray moves to the far right, and the camera takes a picture of the test tube box and stores it in the system. The foam cover is then clamped by the lifting claw assembly and lifted to open the inlet and outlet. When the foam cover is lifted to a certain height, the cover placement assembly rotates to the bottom of the foam cover, and the lifting claw assembly descends to place the foam cover on the cover placement assembly.

[0021] Step 4: The system determines the location of the stored frozen rack, then rotates the turntable to rotate the lifting claw assembly, guide assembly, cover placement assembly, and insertion and transfer assembly to the opposite side of the frozen rack. The guide assembly is then moved to the bottom, and the lifting claw assembly then removes the frozen rack from the tank through the inlet and outlet until the storage cavity corresponding to the number of layers of the frozen rack is moved to the outside of the inlet and outlet.

[0022] Step 5: The insertion and removal plate on the transfer assembly rotates to the support block and extends the insertion and removal plate to the bottom of the test tube box. The support block then moves downward and is placed on the insertion and removal plate. The test tube box on the insertion and removal plate is then removed, rotated in the opposite direction, and placed into the storage chamber of the freezing rack. The freezing rack is then placed back into the tank by the lifting claw assembly, and the inlet and outlet are sealed with a foam cover.

[0023] Step 6: When taking out the test tube box, the system determines the layer number of the target freezing rack, takes it out to the tray in the opposite way as described above, takes a picture of the test tube box, and moves it to the left.

[0024] (3) Beneficial effects

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] In the above scheme, by arranging a lifting clamp assembly, a guide assembly, a cover placement assembly, a test tube box entry and exit assembly, a photo lifting assembly and an insertion and transfer assembly on the tank body, multiple freezing racks in the tank body can be automatically stored and retrieved, which greatly improves the efficiency and accuracy of the biological sample test tube boxes. At the same time, pictures are taken by a camera and stored in the management system for unified management, which can accurately identify the storage location. The entire process is controlled by precise transmission to ensure that the biological sample test tube box is accurately positioned and quickly accessed, reducing the impact of human factors on the temperature and sample quality in the tank body, and avoiding damage to the activity and integrity of the samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the three-dimensional structure of a deep-cold storage and access device for a biological sample test tube box.

[0028] Figure 2 This is a partially enlarged structural diagram of a deep-cold storage and access device used for a biological sample test tube box.

[0029] Figure 3 This is a schematic diagram of the structure of a lifting claw assembly of a deep-cold storage and retrieval device for biological sample test tube boxes.

[0030] Figure 4 This is a schematic diagram of the rear view structure of the lifting claw assembly of the deep-cold storage and retrieval equipment for biological sample test tube boxes.

[0031] Figure 5 This is a schematic diagram of the freezing rack structure of a deep-cold storage and retrieval device for biological sample test tube boxes.

[0032] Figure 6 The diagram is a structural diagram of an insertion and transfer assembly of a cryogenic storage and retrieval device for a biological sample test tube box.

[0033] Figure 7The figure is a bottom-up structural schematic diagram of an insertion and transfer assembly of a cryogenic storage and retrieval device for a biological sample test tube box.

[0034] The markings in the accompanying drawings are: 1. Tank body; 2. Mounting plate; 3. Inlet and outlet; 4. Freezing rack; 5. Foam cover; 6. Turntable; 7. Lifting clamp assembly; 8. Guide assembly; 9. Cover placement assembly; 10. Test tube box inlet and outlet assembly; 11. Photographing lifting assembly; 12. Insertion and transfer assembly; 401. Outer frame; 402. Partition; 403. Hanging rod; 404. Boss; 601. Synchronous gear ring; 701. Bottom plate; 702. Column; 703. First motor; 704. Second motor; 705. Second movable plate; 706. Electric clamp; 707. Clamping claw; 801. Guide frame; 802. Third motor; 803. Vertical screw rod; 804. Guide hole; 805. Roller ;901, fourth motor; 902, placement plate; 903, placement slot; 904, clamping slot; 905, fixed seat; 1001, vertical pole; 1002, entry and exit plate; 1003, tray; 1004, avoidance slot; 1101, rectangular hole; 1102, vertical pole; 1103, base; 1104, camera; 1105, through hole; 1106, column; 1107, top plate; 1108, support block; 1201, lower fixed plate; 1202, gear plate; 1203, upper fixed plate; 1204, movable rod; 1205, plug-in plate; 1206, fifth motor; 1207, sixth motor; 1208, rotating shaft; 1209, rocker arm; 1210, long slot. DETAILED DESCRIPTION

[0035] An embodiment of the present invention provides a cryogenic storage and retrieval device for a biological sample test tube box, comprising a tank body 1. When in use, the tank body 1 is filled with liquid nitrogen (-196°C). A mounting plate 2 is fixedly connected to the upper surface of the tank body 1. The mounting plate 2 is provided with an inlet and outlet 3 communicating with the tank body 1. A plurality of freezing racks 4 are hung around the inlet and outlet 3 and are installed with a foam cover 5. A turntable 6 is mounted on the upper surface of the mounting plate 2. A lifting claw assembly 7, a guide assembly 8, a cover placement assembly 9, and an insertion and transfer assembly 12 are mounted on the upper surface of the turntable 6. A test tube box inlet and outlet assembly 10 is mounted on the lower front surface of the mounting plate 2. A photographing lifting assembly 11 is installed on the inlet and outlet path of the test tube box inlet and outlet assembly 10.

[0036] The turntable 6 is used to drive the lifting clamp assembly 7, the guide assembly 8, the cover placement assembly 9 and the insertion and transfer assembly 12 to rotate. The test tube box in and out assembly 10 is used to move the placed test tube box into the photographing lifting assembly 11. The lifting clamp assembly 7 is used to transfer the foam cover 5 and the freezing rack 4 in the tank body 1. The insertion and transfer assembly 12 is used to transfer the test tube box between the photographing lifting assembly 11 and the freezing rack 4.

[0037] like Figure 2 and Figure 5 As shown, in this embodiment, the freezing rack 4 includes an outer frame 401, one side of the outer frame 401 is provided with an opening, and the inner walls on both sides of the outer frame 401 are fixedly connected to multiple partitions 402 to form multiple storage cavities, the top of the outer frame 401 is fixedly connected to a hanging rod 403, and the edge of the inlet and outlet 3 is fixedly connected to a circle of bosses 404, and the top of the hanging rod 403 is L-shaped and located on the boss 404; in this way, the test tube box can be placed between the left and right partitions 402, and the hanging rod 403 is hung on the positioning groove of the boss 404 to ensure accurate grasping of the freezing rack 4.

[0038] like Figure 1 and Figure 2 As shown, in this embodiment, the lower surface of the turntable 6 is rotatably connected to the mounting plate 2 via a bearing, the outer surface of the turntable 6 is fixedly connected to a synchronous ring gear 601, a drive motor is mounted on the mounting plate 2, the output end of the drive motor is meshed with the synchronous ring gear 601 via a gear, and the turntable 6 is concentrically arranged with the inlet and outlet 3; the drive motor can drive the synchronous ring gear 601 and the turntable 6 to rotate freely, and during the rotation process, the turntable 6 can drive the lifting claw assembly 7, the guide assembly 8, the cover placement assembly 9 and the insertion and transfer assembly 12 to rotate to the opposite side of the freezing rack 4.

[0039] like Figure 2 、 Figure 3 and Figure 4 As shown, in this embodiment, the lifting claw assembly 7 includes a base plate 701 fixedly connected to the upper surface of the turntable 6, a column 702 is slidably provided on the upper surface of the base plate 701, a slider is fixedly connected to the lower surface of the column 702, a guide rail is installed on the upper surface of the base plate 701, a first motor 703 is installed on the right side of the column 702, the output end of the first motor 703 is connected to a horizontal screw rod through a belt drive, the screw rod is threadedly connected to the bottom end of the column 702, a second motor 704 is installed on the top of the column 702 and a second movable plate 705 is slidably provided, and the second movable plate 705 is installed on the top of the column 702. 05 A slider is installed on the right side, a guide rail is installed on the column 702, the output end of the second motor 704 is connected to a vertical screw rod through a belt drive, the screw rod is threadedly connected to the second movable plate 705, an electric clamp 706 is installed on the second movable plate 705, and two clamping claws 707 are installed on the output end of the electric clamp 706; the first motor 703 can drive the column 702 to move horizontally, and the second motor 704 drives the second movable plate 705, the electric clamp 706 and the clamping claw 707 to move up and down, and the two clamping claws 707 can clamp the hanging rod 403 at the top of the outer frame 401.

[0040] like Figure 2 、 Figure 3 and Figure 4As shown, in this embodiment, the guide assembly 8 includes a guide frame 801 slidably connected to the left side of the column 702 and a third motor 802 installed at the top of the column 702. A slider is installed on the right side of the guide frame 801, and a guide rail is installed on the column 702. The output end of the third motor 802 is fixedly connected to a vertical screw rod 803, and the vertical screw rod 803 is threadedly connected to the guide frame 801. A guide hole 804 is opened in the middle of the guide frame 801, and rollers 805 are installed at the four corners of the guide hole 804. The guide frame 801 is driven up and down by the third motor 802. When grabbing the freezing rack 4, the outer frame 401 of the freezing rack 4 can enter the guide hole 804 and be guided by the roller 805, thereby reducing friction and preventing the freezing rack 4 from shaking when being taken out.

[0041] like Figure 1 and Figure 2 As shown, in this embodiment, the cover placement assembly 9 includes a fixed base 905 fixedly connected to the upper surface of the turntable 6, and a fourth motor 901 is installed on the fixed base 905. The output end of the fourth motor 901 is fixedly connected to a placement plate 902, and the upper surface of the placement plate 902 is provided with a placement groove 903 corresponding to the foam cover 5, and the upper surface of the foam cover 5 is provided with two clamping grooves 904; the fourth motor 901 can drive the placement plate 902 to rotate without affecting the movement of the lifting clamping claw assembly 7, and the two clamping claws 707 extend out of the interior of the foam cover 5 to clamp and fix it, and then are placed in the placement groove 903.

[0042] like Figure 1 and Figure 2 As shown, in this embodiment, the test tube box in-and-out assembly 10 includes a driving member and a plurality of vertical rods 1001 fixedly connected to the front side of the lower surface of the mounting plate 2. The bottom end of the vertical rod 1001 is fixedly connected to the in-and-out plate 1002. The upper surface of the in-and-out plate 1002 is slidably mounted with a tray 1003. The driving member is used to drive the tray 1003 to move along the in-and-out plate 1002. The driving member is an electric slide or a motor-driven screw to move the tray 1003, which is not described in detail here. Avoidance grooves 1004 are provided at the four corners of the tray 1003. A sensor is installed on the tray 1003. After the tray 1003 senses that a test tube box is placed in, it can move laterally along the in-and-out plate 1002.

[0043] like Figure 1 and Figure 2As shown, in this embodiment, the photo lifting assembly 11 includes a driving member and a rectangular hole 1101 opened on the front side of the mounting plate 2. Two vertical rods 1102 are fixedly connected to the inner walls on both sides of the front and rear of the rectangular hole 1101. The outer surface of the vertical rod 1102 is slidingly sleeved with a base 1103. The driving member is used to drive the base 1103 to slide up and down along the vertical rod 1102. The driving member is an electric slide or a motor-driven screw to move the base 1103. The specific description is not given here. A camera 1104 is installed on the base 1103. A through hole 1105 corresponding to the camera 1104 is opened in the middle of the access plate 1002. The four corners of the seat 1103 are fixedly connected with columns 1106, the top ends of the columns 1106 pass through the rectangular holes 1101 and are fixedly connected with the top plate 1107, and the sides of the outer surfaces of the four columns 1106 that are close to each other are fixedly connected with support blocks 1108 corresponding to the avoidance grooves 1004; after the test tube box is moved to the top of the support blocks 1108, the support blocks 1108 are moved upward to the avoidance grooves 1004, and the test tube box on the tray 1003 is moved to the upper side of the mounting plate 2, so that the test tube box can move up and down, and at the same time, the camera 1104 takes pictures of the test tube box through the through hole 1105 and stores them in the system.

[0044] like Figure 2 、 Figure 6 and Figure 7 As shown, in this embodiment, the insertion and transfer assembly 12 includes a lower fixed plate 1201 fixedly connected to the upper surface of the turntable 6, the upper surface of the lower fixed plate 1201 is rotatably connected to the upper fixed plate 1203 through a gear plate 1202, the upper surface of the upper fixed plate 1203 is slidably connected to a movable rod 1204, a slider is installed on the lower side of the movable rod 1204, a guide rail is installed on the upper fixed plate 1203, one end of the movable rod 1204 is fixedly connected to the insertion plate 1205, and the lower surface of the other end of the movable rod 1204 is fixedly connected to the insertion plate 1205. A long slot 1210 is provided, and the fifth motor 1206 is fixedly connected to the right side of the upper surface of the lower fixed plate 1201, and the sixth motor 1207 is fixedly connected to the right side of the upper surface of the upper fixed plate 1203. The output end of the fifth motor 1206 is connected to the gear plate 1202 through a belt, and the sixth motor 1207 is connected to the rotating shaft 1208 through a belt transmission. The bottom end of the rotating shaft 1208 is fixedly connected to the rocker arm 1209, and the other end of the rocker arm 1209 is fixedly connected to the bearing and is located inside the long slot 1210.

[0045] The fifth motor 1206 drives the gear plate 1202, the upper fixed plate 1203 and the insertion plate 1205 to rotate to the support block 1108, and then the sixth motor 1207 drives the rotating shaft 1208 and the rocker arm 1209 to rotate. Since the other end of the rocker arm 1209 is fixedly connected to the bearing and located inside the long slot 1210, the upper fixed plate 1203 is slidably connected to the movable rod 1204. At this time, the rocker arm 1209 can drive the movable rod 1204 and the insertion plate 1205 to extend or contract during the movement, thereby extending the insertion plate 1205 to the bottom of the test tube box, and then the support block 1108 moves downward and is placed on the insertion plate 1205. Then, the test tube box on the insertion plate 1205 is taken out, and then rotated in the opposite direction and sent into the storage cavity of the freezing rack 4.

[0046] The technical solutions provided by the present invention are as follows:

[0047] Step 1: Move the support block 1108 on the camera lift assembly 11 to the bottom and wait. Then, move the tray 1003 of the test tube box in and out assembly 10 to the left, and then place the test tube box on the tray 1003.

[0048] Step 2: After the tray 1003 senses that a test tube box has been placed in the tray, it moves to the top of the support block 1108. The support block 1108 then moves upward to the avoidance groove 1004, and the test tube box on the tray 1003 is moved to the upper side of the mounting plate 2.

[0049] Step 3: The tray 1003 moves to the far right, and the camera 1104 takes a picture of the test tube box and stores it in the system. The foam cover 5 is then clamped by the lifting claw assembly 7 and lifted to open the inlet and outlet 3. When the foam cover 5 is lifted to a certain height, the cover placement assembly 9 rotates to the bottom of the foam cover 5, and the lifting claw assembly 7 descends, placing the foam cover 5 on the cover placement assembly 9.

[0050] Step 4: The system determines the position of the stored frozen rack 4, then rotates the turntable 6 to rotate the lifting claw assembly 7, the guide assembly 8, the cover placement assembly 9, and the insertion and transfer assembly 12 to the opposite side of the frozen rack 4, and then moves the guide assembly 8 to the bottom. Then, the lifting claw assembly 7 removes the frozen rack 4 from the tank body 1 through the inlet and outlet 3 until the storage cavity corresponding to the number of layers of the frozen rack 4 moves to the outside of the inlet and outlet 3;

[0051] Step 5: The insertion and removal plate 1205 on the transfer assembly 12 is rotated to the support bracket 1108 and extended to the bottom of the test tube box. The support bracket 1108 is then moved downward and placed on the insertion and removal plate 1205. The test tube box on the insertion and removal plate 1205 is then removed, rotated in the opposite direction, and placed into the storage chamber of the freezing rack 4. The freezing rack 4 is then placed back into the tank body 1 by the lifting claw assembly 7, and the inlet and outlet 3 are sealed by the foam cover 5.

[0052] Step 6: When the test tube box is taken out, the system determines the layer number of the target freezing rack 4, takes it out to the tray 1003 in the opposite way as described above, takes a photo of the test tube box, and then moves it to the left.

[0053] All technical features in this embodiment can be freely combined according to actual needs.

[0054] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present technical solution is within the scope of protection of the present invention.

Claims

1. A cryogenic storage and retrieval device for a biological sample test tube box, characterized in that: The invention comprises a tank body (1), the upper surface of the tank body (1) is fixedly connected to a mounting plate (2), the mounting plate (2) is provided with an inlet and outlet (3) connected to the tank body (1), a plurality of freezing racks (4) are hung around the inlet and outlet (3) and a foam cover plate (5) is installed, the upper surface of the mounting plate (2) is provided with a turntable (6), the upper surface of the turntable (6) is provided with a lifting claw assembly (7), a guide assembly (8), a cover plate placement assembly (9) and an insertion and transfer assembly (12), the front lower surface of the mounting plate (2) is provided with a test tube box inlet and outlet assembly (10), and a photographing lifting assembly (11) is installed on the inlet and outlet path of the test tube box inlet and outlet assembly (10); The turntable (6) is used to drive the lifting clamp assembly (7), the guide assembly (8), the cover placement assembly (9) and the insertion and transfer assembly (12) to rotate; the test tube box inlet and outlet assembly (10) is used to move the placed test tube box into the photographing lifting assembly (11); the lifting clamp assembly (7) is used to transfer the foam cover (5) and the freezing rack (4) in the tank body (1); and the insertion and transfer assembly (12) is used to transfer the test tube box between the photographing lifting assembly (11) and the freezing rack (4).

2. The deep-cold storage and access device for a biological sample test tube box according to claim 1, characterized in that: The freezing rack (4) includes an outer frame (401), one side of the outer frame (401) is provided with an opening, the inner walls on both sides of the outer frame (401) are fixedly connected with a plurality of partitions (402) to form a plurality of storage cavities, the top end of the outer frame (401) is fixedly connected with a hanging rod (403), the edge of the inlet and outlet (3) is fixedly connected with a circle of bosses (404), and the top end of the hanging rod (403) is L-shaped and is located on the boss (404).

3. The cryogenic storage and retrieval device for a biological sample test tube box according to claim 2, wherein the lower surface of the turntable (6) is rotatably connected to the mounting plate (2) via a bearing, the outer surface of the turntable (6) is fixedly connected to a synchronous ring gear (601), a drive motor is mounted on the mounting plate (2), the output end of the drive motor is meshed with the synchronous ring gear (601) via a gear, and the turntable (6) is concentrically arranged with the inlet and outlet (3).

4. The deep-cold storage and access device for a biological sample test tube box according to claim 1, characterized in that: The lifting clamp assembly (7) includes a base plate (701) fixedly connected to the upper surface of the turntable (6), a column (702) is slidably provided on the upper surface of the base plate (701), a first motor (703) is installed on the right side of the column (702), the output end of the first motor (703) is connected to a horizontal screw rod through a belt transmission, the screw rod is threadedly connected to the bottom end of the column (702), a second motor (704) is installed on the top of the column (702) and a second movable plate (705) is slidably provided, the output end of the second motor (704) is connected to a vertical screw rod through a belt transmission, the screw rod is threadedly connected to the second movable plate (705), an electric clamp (706) is installed on the second movable plate (705), and two clamping claws (707) are installed on the output end of the electric clamp (706).

5. The deep-cold storage and access device for a biological sample test tube box according to claim 4, characterized in that: The guide assembly (8) comprises a guide frame (801) slidably connected to the left side of the column (702) and a third motor (802) mounted on the top of the column (702); an output end of the third motor (802) is fixedly connected to a vertical screw rod (803); the vertical screw rod (803) is threadedly connected to the guide frame (801); a guide hole (804) is provided in the middle of the guide frame (801); rollers (805) are mounted at the four corners of the guide hole (804).

6. The deep-cold storage and access device for a biological sample test tube box according to claim 5, characterized in that: The cover plate placement assembly (9) comprises a fixing seat (905) fixedly connected to the upper surface of the turntable (6); a fourth motor (901) is mounted on the fixing seat (905); an output end of the fourth motor (901) is fixedly connected to a placement plate (902); a placement groove (903) corresponding to the foam cover plate (5) is provided on the upper surface of the placement plate (902); and two clamping grooves (904) are provided on the upper surface of the foam cover plate (5).

7. The deep-cold storage and access device for a biological sample test tube box according to claim 1, characterized in that: The test tube box inlet and outlet assembly (10) comprises a driving member and a plurality of vertical rods (1001) fixedly connected to the front side of the lower surface of the mounting plate (2); the bottom ends of the vertical rods (1001) are fixedly connected to an inlet and outlet plate (1002); a tray (1003) is slidably mounted on the upper surface of the inlet and outlet plate (1002); the driving member is used to drive the tray (1003) to move along the inlet and outlet plate (1002); and avoidance grooves (1004) are provided at the four corners of the tray (1003).

8. The deep-cold storage and access device for a biological sample test tube box according to claim 7, characterized in that: The photographing lifting assembly (11) comprises a driving member and a rectangular hole (1101) provided on the front side of the mounting plate (2); two vertical rods (1102) are fixedly connected to the inner walls on both the front and rear sides of the rectangular hole (1101); a base (1103) is provided on the outer surface of the vertical rod (1102); the driving member is used to drive the base (1103) to slide up and down along the vertical rod (1102); a camera (1104) is installed on the base (1103); A through hole (1105) corresponding to the camera (1104) is provided in the middle of the entry and exit plate (1002); four corners of the base (1103) are fixedly connected to columns (1106); the top ends of the columns (1106) pass through the rectangular hole (1101) and are fixedly connected to the top plate (1107); and the adjacent sides of the outer surfaces of the four columns (1106) are fixedly connected to support blocks (1108) corresponding to the avoidance groove (1004).

9. The deep-cold storage and access device for a biological sample test tube box according to claim 1, characterized in that: The insertion and removal transfer assembly (12) comprises a lower fixed plate (1201) fixedly connected to the upper surface of the turntable (6); the upper surface of the lower fixed plate (1201) is rotatably connected to the upper fixed plate (1203) via a gear plate (1202); the upper surface of the upper fixed plate (1203) is slidably connected to a movable rod (1204); one end of the movable rod (1204) is fixedly connected to the insertion and removal plate (1205); the lower surface of the other end of the movable rod (1204) is provided with a long groove (1210); the lower surface of the lower fixed plate (1201) is provided with a plurality of movable rods (1204); and the movable rod (1204) is provided with a plurality of movable rods (1205). ) is fixedly connected to the right side of the upper surface of the upper fixed plate (1203), and the sixth motor (1207) is fixedly connected to the right side of the upper surface of the upper fixed plate (1203). The output end of the fifth motor (1206) is connected to the gear plate (1202) through a belt, and the sixth motor (1207) is connected to the rotating shaft (1208) through a belt transmission. The bottom end of the rotating shaft (1208) is fixedly connected to a rocker (1209), and the other end of the rocker (1209) is fixedly connected to a bearing and is located inside the long slot (1210).

10. A cryogenic storage and access method for a biological sample test tube box according to any one of claims 1 to 9, characterized in that: The steps include: Step 1: Move the support block (1108) on the photographing lifting assembly (11) to the bottom end and wait, then move the tray (1003) of the test tube box in and out assembly (10) to the left, and then place the test tube box on the tray (1003); Step 2: After the tray (1003) senses that a test tube box has been placed in the tray, it moves to the top of the support block (1108), then the support block (1108) moves upward to the avoidance groove (1004), and the test tube box on the tray (1003) is moved to the upper side of the mounting plate (2); Step 3: The tray (1003) moves to the rightmost side, and then the camera (1104) takes a picture of the test tube box and stores it in the system. Then, the foam cover (5) is clamped by the lifting claw assembly 7 and lifted to open the inlet and outlet (3). When the foam cover (5) is lifted to a certain height, the cover placement assembly (9) rotates to the bottom of the foam cover (5), and the lifting claw assembly (7) descends to place the foam cover (5) on the cover placement assembly (9); Step 4: The system determines the position of the stored frozen storage rack (4), then rotates the turntable (6) to rotate the lifting claw assembly (7), the guide assembly (8), the cover placement assembly (9) and the insertion and transfer assembly (12) to the opposite side of the frozen storage rack (4), and then moves the guide assembly (8) to the bottom. Then, the lifting claw assembly 7 takes out the frozen storage rack (4) in the tank body (1) through the inlet and outlet (3) until the storage cavity corresponding to the number of layers of the frozen storage rack (4) moves to the outside of the inlet and outlet (3); Step 5: The insertion plate (1205) on the insertion and transfer assembly (12) is rotated to the support block (1108), and the insertion plate (1205) is extended to the bottom of the test tube box. The support block (1108) is then moved downward and placed on the insertion plate (1205). The test tube box on the insertion plate (1205) is then taken out, rotated in the opposite direction, and placed into the storage cavity of the freezing rack (4). The freezing rack (4) is then placed back into the tank body (1) by the lifting claw assembly (7), and the inlet and outlet (3) are sealed by the foam cover (5); Step 6: When the test tube box is taken out, the system determines the number of layers of the target freezing rack (4), takes it out to the tray (1003) in the opposite manner as described above, takes a photo of the test tube box, and then moves it to the left.

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