Simulation test device and method for enhanced control of pathogenic bacteria in secondary water supply of building

Through modular multi-material pipelines and dual-stage ultraviolet collaborative disinfection system, the problems of single simulation of multi-material pipelines and single disinfection methods in the existing technology are solved, and efficient control and accurate simulation of pathogenic bacteria in the building secondary water supply system is achieved.

CN120442374APending Publication Date: 2025-08-08GUANGXI UNIV FOR NATITIES
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Patent Information

Application Number
CN202510643099.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-19
Publication Date
2025-08-08

AI Technical Summary

Technical Problem

The existing technology cannot truly simulate the multi-material pipeline environment. The traditional disinfection methods are single and cumbersome, and biofilm sampling is easy to destroy, resulting in poor control effect of pathogenic bacteria.

Method used

Modular multi-material pipelines, dual-stage ultraviolet collaborative disinfection and intelligent monitoring systems are adopted to achieve high-precision control of pathogenic bacteria through detachable pipelines and multi-parameter coupling.

Benefits of technology

It realizes rapid replacement and efficient disinfection of multi-material pipelines, improves test efficiency and result accuracy, and enhances the inactivation effect of pathogenic bacteria in biofilms.

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Abstract

The invention discloses a simulation test device and method for enhanced control of pathogenic bacteria in secondary water supply of a building, and belongs to the technical field of water treatment and microorganism safety control. The simulation test device comprises a valve, a secondary water supply tank is arranged on one side of the valve, and a starting end overflowing type ultraviolet disinfection reactor is arranged on the side, away from the valve, of the secondary water supply tank; a polyvinyl chloride (PVC) pipe, a stainless steel pipe and a copper pipe which are connected in parallel are arranged on one side, far away from the secondary water supply tank, of the initial end overflowing type ultraviolet disinfection reactor, and a faucet end ultraviolet disinfection reactor and a faucet are arranged on one side, far away from the overflowing type ultraviolet disinfection reactor, of the pipes made of different materials. According to the invention, multi-parameter regulation and control such as disinfection mode switching and pipe selection are integrated, a biological membrane in-situ acquisition system is innovatively configured, and an efficient experimental platform is provided for researching a transmission mechanism and a control strategy of pathogenic bacteria.
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Description

Technical Field

[0001] The present invention relates to the technical field of microbial safety control of building secondary water supply systems, and specifically to a simulation test device and method for enhanced control of pathogenic bacteria in building secondary water supply, which is suitable for water treatment process optimization, pipeline material selection and disinfection strategy development. Background Art

[0002] The secondary water supply system in old urban communities and high-rise buildings is the "last mile" of safe water supply for the public. It is delivered to users through short-distance water supply pipelines through pressurized storage. However, due to problems such as residual chlorine attenuation or lack of effective disinfection during the water storage process, microorganisms can easily grow and multiply in the water supply pipelines and form biofilms - a "reservoir" for drinking water pathogens. The formation of biofilms mainly includes three stages: attachment, growth and shedding. Once the biofilm falls off and releases pathogens into the water body, it will increase the biological risk of tap water quality and pose a serious threat to public health. Although the existing technology has proposed a variety of disinfection and antibacterial methods, there are still significant defects in actual application, which are specifically manifested in the following aspects.

[0003] First, the simulation of a single pipe material is limited. Most test devices utilize only a single pipe material, failing to truly simulate the complex environment of multiple pipe materials found in real-world projects. Different materials exhibit significant differences in their effectiveness on biofilm formation and bacterial inhibition. For example, copper (Cu) pipes have natural antibacterial properties due to the release of copper ions, while polyvinyl chloride (PVC) pipes are more susceptible to biofilm growth due to their high surface roughness. However, existing technologies lack modular design, making it impossible to quickly switch pipe materials to compare their antibacterial properties.

[0004] Second, traditional disinfection methods often rely on a single method, such as NaClO / ClO2 disinfection or ultraviolet (UV) disinfection, without integrating the synergistic effects of these two methods to inactivate bacteria within biofilms. Residual chlorine is easily attenuated during long-distance pipeline transportation, and bacteria within biofilms are more tolerant to chlorine due to the protective effect of extracellular polymeric substances (EPS). UV disinfection is effective in inactivating free bacteria, but it is difficult to penetrate biofilms and inactivate bacteria within.

[0005] Third, traditional devices often use fixed pipes. Replacing pipes of different materials requires disassembling the entire system, which takes hours and easily introduces contamination, making rapid comparative testing difficult. Furthermore, biofilm sampling often relies on scraping or offline culture, which disrupts the biofilm structure and can lead to detection errors.

[0006] To address these shortcomings, this paper proposes a modular, multi-parameter coupled simulation test device and method. By utilizing detachable, multi-material piping, dual-stage UV disinfection, and an intelligent monitoring system, this device achieves high-precision control of pathogens and optimizes antibacterial strategies. This approach aims to fill a gap in existing technology and provide a scientific and reliable testing platform for engineering design, piping selection, and disinfection strategy development for secondary water supply systems. Summary of the Invention

[0007] This invention relates to a simulation testing device and method for enhanced pathogen control in building secondary water supplies. The goal is to provide a device that can effectively simulate and evaluate the effectiveness of pathogen control in secondary water supply systems. The device simulates an actual water supply environment, treating chlorinated tap water and inoculating it with a mixed bacterial suspension of Escherichia coli and Pseudomonas aeruginosa. Through multi-stage disinfection and transport through pipes of different materials, the device ultimately performs secondary disinfection at the user's tap to evaluate pathogen survival rates and control effectiveness under different conditions.

[0008] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a valve, a secondary water supply tank is provided on one side of the valve, a starting-end overflow type ultraviolet disinfection reactor is provided on the side of the secondary water supply tank away from the valve, a pressure pump is provided on the side of the starting-end overflow type ultraviolet disinfection reactor away from the secondary water supply tank, and parallel water supply pipes are provided on the side of the pressure pump away from the starting-end overflow type ultraviolet disinfection reactor, including three kinds of detachable pipes made of PVC, stainless steel and copper, and a faucet-end ultraviolet disinfection reactor and a faucet are provided on the side of the detachable pipes made of different pipes away from the pressure pump.

[0009] As a preferred embodiment, the starting-end overflow ultraviolet disinfection reactor includes an overflow ultraviolet disinfection metal shell, a quartz sleeve is provided in the overflow ultraviolet disinfection metal shell, an ultraviolet disinfection lamp is provided in the quartz sleeve, a compression spring is provided on the left side of the quartz sleeve, and power supplies are provided on both sides of the overflow ultraviolet reactor. The ultraviolet dose of the starting-end overflow ultraviolet disinfection reactor is 40 mJ / cm 2 .

[0010] As a preferred embodiment, the faucet-end UV disinfection reactor is built into the water supply pipe and connected to the faucet. The faucet is connected to the faucet-end UV disinfection reactor through a pipe to ensure that the water flow is disinfected by UV before entering the faucet. The faucet-end UV disinfection reactor is equipped with a UV lamp with a UV dose of 20 mJ / cm 2 .

[0011] As a preferred embodiment, parallel detachable water supply pipes are configured, including PVC pipes, stainless steel pipes, and copper pipes. Flange joints are used to enable quick removal and replacement, and the inner wall roughness is consistent with actual engineering pipes (PVC: Ra = 0.8 μm, stainless steel: Ra = 0.2 μm, copper: Ra = 0.5 μm). The flange joints include a first flange and a second flange, with a soft PTFE sealing gasket installed between the flanges to provide a leak-proof seal.

[0012] As a preferred embodiment, chlorinated tap water enters the secondary water supply tank through a valve. The water then flows through a flow-through UV disinfection reactor at the start for primary disinfection. It is then transported to the water supply pipeline by a pressure pump. After disinfection, the water flows through three parallel detachable pipes: PVC pipes, stainless steel pipes, and copper pipes, simulating a building's secondary water supply network. A faucet-end UV disinfection reactor is installed before the faucet for secondary disinfection, and the water is finally discharged through the user's tap. Water intakes are set up along the device, and the pipes of different materials are regularly disassembled to collect biofilm samples on the inner wall. The biofilm characteristics and the number of pathogens (Escherichia coli and Pseudomonas aeruginosa) are analyzed.

[0013] As a preferred embodiment, the method comprises the following steps: S1. Select water supply pipes of different materials, connect and fix them section by section through flange joints, and check for water leakage; S2. Fix and install the UV disinfection reactors at the beginning and faucet ends of the water supply pipeline and the water intakes along the pipeline; S3. Add a mixed bacterial suspension of Escherichia coli and Pseudomonas aeruginosa to the secondary water supply tank to simulate the growth characteristics of biofilm pathogens in actual water supply pipes and strengthen control measures; S4. Depending on the specific requirements of use, choose whether to start the UV disinfection reactor. It can also be used in conjunction with sodium hypochlorite (NaClO) / chlorine dioxide (ClO2) to form a UV / chemical disinfection combination process, including NaClO / ClO2 chemical disinfection only, UV-NaClO / ClO2 chemical disinfection at the start end, UV-NaClO / ClO2 chemical disinfection at the tap end, and a combination of UV-NaClO / ClO2 chemical disinfection at the start end and the tap end. By changing parameters such as the type and dosage of disinfectants, research can be conducted to control pathogens in the secondary water supply of buildings under different disinfection processes. S5. Start the device, supply water continuously, and regularly take tap water samples and water samples along the way to analyze changes in water quality indicators; S6. Regularly dismantle pipes made of different materials and collect biofilm samples from the inner wall to obtain in situ cultured biofilms grown using different disinfection methods, different pipe materials, or different pipe locations, and then analyze the formation and regulation principles of biofilms.

[0014] Compared with the existing technology, the advantages of the present invention are: (1) the rapid disassembly and replacement of PVC, stainless steel and copper pipes can be achieved through flange joints, supporting multi-material parallel testing and greatly improving the test efficiency; (2) the ultraviolet light at the beginning inactivates free bacteria, and the ultraviolet light at the end blocks secondary pollution at the user end. The ultraviolet light-enhanced NaClO / ClO2 effect is more effective in inactivating pathogenic bacteria in biofilms than the traditional single disinfection method; (3) it has flexible and controllable test conditions. Through components such as valves, pumps and sampling ports, test operations and data collection can be conveniently carried out to ensure the accuracy and reliability of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0016] Figure 1 System diagram of the simulation test device for enhanced control of pathogens in secondary water supply of buildings; Figure 2 This is a schematic diagram of an initial over-flow ultraviolet disinfection reactor device; Figure 3 This is a schematic diagram of the UV disinfection reactor device at the faucet end; Figure 4 This is a schematic diagram of a detachable pipe flange joint. Figure 5 The inactivation effect of pathogens in the secondary water supply pipes without UV and low NaClO; Figure 6 The inactivation effect of pathogenic bacteria in the secondary water supply pipe without UV and low ClO2; Figure 7 The inactivation effect of Pseudomonas aeruginosa in secondary water supply pipes treated with UV-enhanced NaClO disinfection at the starting and tap ends; Figure 8 The inactivation effect of Pseudomonas aeruginosa in secondary water supply of pipelines under UV-enhanced ClO2 disinfection treatment at the starting and tap ends; Figure 9 The changes of Pseudomonas aeruginosa in secondary water supply pipes of different materials under the action of UV irradiation at the initial end; Figure 10 The changes of Pseudomonas aeruginosa in secondary water supply pipes of different materials under UV irradiation at the tap end; Figure 11 The changes of Pseudomonas aeruginosa in secondary water supply pipes made of different materials under UV irradiation at the starting and faucet ends.

[0017] Legend: Valve; 2. Secondary water supply tank; 3. Sampling port; 4. Start-end over-flow UV disinfection reactor; 5. Pressure pump; 6. PVC pipe; 7. Stainless steel pipe; 8. Copper pipe; 9. Faucet-end UV disinfection reactor; 10. Faucet; 11. Compression spring; 12. UV disinfection lamp; 13. Quartz sleeve; 14. UV disinfection metal casing; 15. Power supply; 16. First flange; 17. Second flange; 18. Soft PTFE sealing gasket. DETAILED DESCRIPTION

[0018] In order to clearly explain the technical principles, implementation plans and innovative value of this patent, a systematic explanation is now given in conjunction with the accompanying drawings and specific implementation cases. It should be pointed out that the embodiments listed in this article only constitute an exemplary display of the technical solution and do not constitute a limitation on the scope of patent protection. According to the relevant provisions of the Patent Law, any equivalent replacement, technical extension or adaptive improvement based on the core principle of this technology, as long as it does not deviate from the innovative essence of the invention, is automatically included in the protection scope of this patent right. After fully understanding the key points of the technology, various derivative schemes achieved by conventional technical means by those skilled in the art are subject to the constraints and protection of the legal effect of this patent.

[0019] It should be noted that the illustrations and embodiments of the present invention primarily illustrate the core design concept of the technical solution. Based on this design concept, the specific implementation forms such as the relevant connection relationships, location layout, power unit, power supply system, hydraulic system, and control system may not be fully described in detail. However, it should be pointed out that those with conventional technical knowledge in this field, provided they fully understand the technical solution of the present invention, are fully capable of implementing the specific configuration of the above-mentioned systems and components using common industry techniques.

[0020] When an element is referred to as being “fixed to” or “disposed on” another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being “connected to” another element, it can be directly connected to the other element or indirectly connected to the other element.

[0021] It should be noted that the definitions of "inside" and "outside" in the orientation descriptions involved in this specification refer to the relative spatial relationships of the component's main structural outline. Other orientation terms such as "length," "width," and "up, down, front, back, left, right, vertical, horizontal, top, and bottom" are expressed with reference to the spatial orientations specified in the drawings in the specification. These orientation limitations are solely for the purpose of standardization and simplicity in the description of technical features and are not restrictive requirements on the actual structure of the product. Those skilled in the art should understand that the relevant orientation descriptions do not constitute technical constraints on the spatial arrangement and installation direction of the device components.

[0022] For ease of description, spatial orientation terms such as "above," "above," "on the upper surface of," and "top" may be used to describe the relative positional relationships between the various elements or structures in the accompanying drawings. It should be understood that such orientation terms not only encompass the illustrated orientation, but also cover the different orientation states that may occur in the elements during actual use or operation. For example, when the device shown in the accompanying drawings is inverted, the elements originally described as "above / above other elements or structures" will be converted to an orientation relationship of "below / beneath other elements or structures." Therefore, for the exemplary term "above," its actual connotation should include both "above" and "below" relative position states. For other possible positioning methods of the device, the spatial relative descriptions described herein should also be interpreted accordingly.

[0023] The ordinal numbers "first" and "second" used in this specification are for descriptive purposes only and should not be interpreted as indicating or implying relative importance, nor as limiting the quantity of the technical features referred to. Accordingly, any feature item with qualifiers such as "first" and "second" explicitly or implicitly includes one or more of such features. In the technical solutions of this invention, "multiple" specifically refers to two or more units of quantity, while "several" refers to one or more units of quantity. Unless otherwise specified, this interpretation shall prevail.

[0024] The simulation test device and method for enhanced control of pathogenic bacteria in secondary water supply of buildings provided by the present invention are now described. Example 1

[0025] like Figure 1 - Figure 4 As shown, the present invention provides a technical solution: a simulation test device and method for enhanced control of pathogenic bacteria in secondary water supply of buildings, comprising: a secondary water supply tank 2 is provided on one side of the valve 1, a starting-end overflow type ultraviolet disinfection reactor 4 is provided on the side of the secondary water supply tank 2 away from the valve 1, a pressure pump 5 is provided on the side of the starting-end overflow type ultraviolet disinfection reactor 4 away from the secondary water supply tank 2, a parallel water supply pipe is provided on the side of the pressure pump 5 away from the starting-end overflow type ultraviolet disinfection reactor 4, three different pipe materials of detachable pipes, polyvinyl chloride 6, stainless steel 7 and copper 8, and a faucet-end ultraviolet disinfection reactor 9 and a faucet 10 are provided on the side of the detachable pipes of different pipe materials away from the pressure pump 5.

[0026] In this embodiment, chlorine-containing tap water disinfected with NaClO / ClO2 first passes through the secondary water supply tank 2 through valve 1. The overflow ultraviolet disinfection reactor 4 at the beginning of the water supply pipeline and the ultraviolet disinfection reactor 9 at the tap end are not started. Only NaClO / ClO2 chemical disinfection is adopted. The water flows through three detachable pipes, namely, a PVC pipe 6, a stainless steel pipe 7, and a copper pipe 8, which are connected in parallel to simulate the secondary water supply network of a building. Finally, the water is discharged through the user's tap 10. The device is then operated continuously, and water samples from the tap and along the way are taken regularly to analyze the changes in the secondary water supply quality under the action of chlorine disinfection. Figure 5 The figure shows the inactivation effect of pathogens in the secondary water supply pipe without UV and low NaClO. Figure 6 The figure shows the inactivation effect of pathogenic bacteria in the secondary water supply pipe without UV and low ClO2. Example 2

[0027] In this embodiment, the UV disinfection reactors at the beginning of the water supply pipe and the tap end are started simultaneously and kept running continuously. The UV dose at the beginning is 40 mJ / cm 2 The UV dose at the faucet end is 20 mJ / cm 2 The chlorinated tap water disinfected by NaClO or ClO2 enters the secondary water supply tank 2, and is initially disinfected by the over-flow UV disinfection reactor 4 at the starting end. After disinfection, the water flows through three detachable pipes, namely, the PVC pipe 6, the stainless steel pipe 7 and the copper pipe 8, which are connected in parallel, and then disinfected by the UV disinfection reactor 9 at the faucet end, and finally flows out through the user's faucet 10. The rest is the same as the specific implementation example 1. The device is continuously operated, and water samples from the tap and along the way are taken regularly. The results are as follows Figure 7 The figure shows the inactivation effect of Pseudomonas aeruginosa in the secondary water supply of the pipeline under UV-enhanced NaClO disinfection treatment at the starting and tap ends. Figure 8 The figure shows the inactivation effect of Pseudomonas aeruginosa in the secondary water supply of the pipeline under UV-enhanced ClO2 disinfection treatment at the starting and faucet ends. Example 3

[0028] Adopt NaClO / ClO2-UV combined disinfection at the beginning. Keep the faucet end UV disinfection reactor 9 closed, start the water supply pipe beginning flow type UV disinfection reactor 4 and keep it running continuously, the UV dose is 40 mJ / cm 2 The chlorinated tap water enters the secondary water supply tank 2 through valve 1, and then is disinfected by the flow-through UV disinfection reactor 4 at the beginning of the water supply pipeline, and then enters three different material water supply pipelines and is transported to the tap 10. The rest is the same as the specific implementation example 1. The continuous operation device regularly takes tap water samples and water samples along the process. The results are as follows Figure 9 Shown are the changes in Pseudomonas aeruginosa in secondary water supply pipes made of different materials under the action of UV irradiation at the initial end. Example 4

[0029] Use NaClO / ClO2-faucet end UV combined disinfection. Keep the over-flow UV disinfection reactor 4 at the beginning of the water supply pipe closed, start the faucet end UV disinfection reactor 9 and keep it running continuously, with a UV dose of 20 mJ / cm 2 The chlorinated tap water enters the secondary water supply tank 2 through valve 1, directly enters the water supply pipe, and then is disinfected by the faucet end ultraviolet disinfection reactor 9, and finally delivered to the faucet 10. The rest is the same as the specific implementation example 1. The continuous operation device regularly takes tap water samples and water samples along the way. The results are as follows Figure 10 Shown are the changes in Pseudomonas aeruginosa in secondary water supply pipes made of different materials under UV irradiation at the faucet end. Example 5

[0030] Use NaClO / ClO2- UV combined disinfection at the start and tap end. Simultaneously start the water supply pipe start 4 and tap end UV disinfection reactor 9 and keep them running continuously. The UV dose at the start is 40 mJ / cm 2 The UV dose at the faucet end is 20mJ / cm 2 The chlorinated tap water enters the secondary water supply tank 2 through valve 1, then passes through the flow-through UV disinfection reactor 4 at the beginning of the water supply pipeline, then enters three water supply pipelines made of different materials, then passes through the UV disinfection reactor 9 at the tap end, and finally is delivered to the tap 10. The rest is the same as in the specific implementation example 1. The device is continuously operated, and tap water samples and water samples along the way are taken regularly. The results are as follows Figure 11 Shown are the changes in Pseudomonas aeruginosa in secondary water supply pipes made of different materials under UV irradiation at the starting and faucet ends.

[0031] The above description is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any technical solution that falls within the spirit and basic principles of the present invention, whether implemented by design modification, equivalent replacement, or improved technical means, shall be included in the scope of protection of the claims of this patent application.

[0032] It should be noted that the terminology used herein is intended solely to illustrate specific implementations and is not intended to limit the exemplary embodiments described herein. Unless otherwise expressly stated, singular nouns should be interpreted as including the plural. It should also be understood that the terms "including" and "comprising" used in this specification are intended to clearly specify the technical features, implementation steps, operational procedures, device components, and / or combinations thereof.

[0033] Unless otherwise specified, the topological structures, mathematical expressions and parameter values of the components and operating procedures described in the various embodiments of the present technical solution should not be regarded as limiting the scope of protection of this application. It should be noted that, in order to clearly present the technical solution, the geometric dimensions of the components in the diagram may be scaled and not drawn strictly in accordance with the actual proportions. Conventional technical contents known in the art may not be described in detail, but they should be deemed to be within the scope of the specification where applicable. In the embodiments involved herein, any specific parameters should be understood as exemplary and do not constitute a constraint. Different exemplary implementation methods may adopt differentiated numerical configurations. Special note: The same or similar identifiers represent similar elements. When an element has been defined in a previous diagram, it will not be repeated in subsequent diagrams in principle.

Claims

1. A simulation test device for enhanced control of pathogenic bacteria in secondary water supply of buildings, characterized in that: include: A valve (1) is provided on one side of the valve (1), a secondary water supply tank (2) is provided on the side of the secondary water supply tank (2) away from the valve (1), a starting-end overflow type ultraviolet disinfection reactor (4) is provided on the side of the starting-end overflow type ultraviolet disinfection reactor (4) away from the secondary water supply tank (2), a pressure pump (5) is provided on the side of the pressure pump (5) away from the starting-end overflow type ultraviolet disinfection reactor (4), and parallel water supply pipes are provided on the side of the pressure pump (5) away from the starting-end overflow type ultraviolet disinfection reactor (4), including three different pipe materials, detachable pipes, namely PVC (6), stainless steel (7) and copper (8), and a faucet end ultraviolet disinfection reactor (9) and a faucet (10) are provided on the side of the detachable pipes of different pipe materials away from the pressure pump (5).

2. A simulation test device for enhanced control of pathogenic bacteria in secondary water supply of buildings according to claim 1, characterized in that: The starting-end overflow ultraviolet disinfection reactor (4) comprises an overflow ultraviolet disinfection metal shell (14), a quartz sleeve (13) is provided in the overflow ultraviolet disinfection metal shell (14), an ultraviolet disinfection lamp (12) is provided in the quartz sleeve (13), a compression spring (11) is provided on the left side of the quartz sleeve (13), power supplies (15) are provided on both sides of the overflow ultraviolet reactor (4), and the ultraviolet dose of the starting-end overflow ultraviolet disinfection reactor (4) is 40 mJ / cm 2 .

3. The simulation test device for enhanced control of pathogenic bacteria in secondary water supply of a building according to claim 1, characterized in that: The faucet-end ultraviolet disinfection reactor (9) is built into the water supply pipe and connected to the faucet (10). The faucet (10) is connected to the faucet-end ultraviolet disinfection reactor (9) through a pipe to ensure that the water flow is disinfected by ultraviolet light before entering the faucet. The faucet-end ultraviolet disinfection reactor (9) is equipped with an ultraviolet lamp with an ultraviolet dose of 20 mJ / cm 2 .

4. The simulation test device for enhanced control of pathogenic bacteria in secondary water supply of a building according to claim 1, characterized in that: Parallel detachable water supply pipes are configured, including PVC pipes (6), stainless steel pipes (7) and copper pipes (8), which can be quickly disassembled and replaced through flange joints, and the inner wall roughness is consistent with that of actual engineering pipes (PVC: Ra=0.8 μm, stainless steel: Ra=0.2 μm, copper: Ra=0.5 μm). The flange joints include a first flange (16) and a second flange (17), and a soft PTFE sealing gasket (18) is provided between the flanges to play a role in sealing and leak prevention.

5. The simulation test device for enhanced control of pathogenic bacteria in secondary water supply of a building according to claim 1, characterized in that: Chlorinated tap water enters the secondary water supply tank (2) through a valve (1), and then flows through a flow-through ultraviolet disinfection reactor (4) at the start for primary disinfection. The water is then transported to the water supply pipe by a pressure pump (5). After disinfection, the water flows through three detachable pipes, namely, a parallel PVC pipe (6), a stainless steel pipe (7), and a copper pipe (8), to simulate a secondary water supply network of a building. A faucet-end ultraviolet disinfection reactor (9) is set in front of the faucet for secondary disinfection, and finally the water is discharged through the user's faucet (10). Water intakes (3) are set along the device. Pipes of different materials are regularly dismantled and inner wall biofilm samples are collected to analyze the biofilm characteristics and the number of pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa).

6. A method for using a simulation test device for enhanced control of pathogenic bacteria in secondary water supply of a building according to any one of claims 1 to 5, characterized in that: The steps include: S1. Select water supply pipes of different materials, connect and fix them section by section through flange joints, and check for water leakage; S2. Fix and install the UV disinfection reactors at the beginning and faucet ends of the water supply pipeline and the water intakes along the pipeline; S3. Add a mixed bacterial suspension of Escherichia coli and Pseudomonas aeruginosa to the secondary water supply tank to simulate the growth characteristics of biofilm pathogens in actual water supply pipes and strengthen control measures; S4. Depending on the specific requirements of use, choose whether to start the UV disinfection reactor. It can also be used in conjunction with sodium hypochlorite (NaClO) / chlorine dioxide (ClO2) to form a UV / chemical disinfection combination process, including NaClO / ClO2 chemical disinfection only, UV-NaClO / ClO2 chemical disinfection at the start end, UV-NaClO / ClO2 chemical disinfection at the tap end, and a combination of UV-NaClO / ClO2 chemical disinfection at the start end and the tap end. By changing parameters such as the type and dosage of disinfectants, research can be conducted to control pathogens in the secondary water supply of buildings under different disinfection processes. S5. Start the device, supply water continuously, and regularly take tap water samples and water samples along the way to analyze changes in water quality indicators; S6. Regularly dismantle pipes made of different materials and collect samples of biofilm on the inner wall to obtain in situ cultured biofilms grown using different disinfection methods, different pipe materials, or different pipe locations, and then analyze the formation and regulation principles of biofilms.