Compound microbial agent capable of degrading organic herbicides and preparation method of compound microbial agent

Abstract

The invention discloses a compound microbial agent capable of degrading organic herbicides and a preparation method of the compound microbial agent, and relates to the technical field of preparation of compound microbial agents. The mass ratio of the glycoside compound to the potassium carbonate is (1: 5)-(1: 10); the synergist is a plant source surfactant, and the addition amount of the synergist is 0.1%-0.5% of the total mass; the photosensitive stabilizer is used for enhancing the persistence of the pesticide effect under sunlight, and the addition amount of the photosensitive stabilizer is 0.05%-0.2%; the plant source surfactant is tea saponin; the photosensitive stabilizer is nano zinc oxide; the preparation method comprises the following steps: synergistically dissolving potassium carbonate and glucoside by adopting a step-by-step dissolution method, firstly dissolving potassium carbonate in warm water at 40-50 DEG C, then adding glucoside, and stirring for 30 minutes at a constant temperature to obtain a primary complex solution; performing nanocrystallization treatment on the primary composite liquid by adopting an ultrasonic dispersion technology to enable the particle size of the liquid medicine to be less than or equal to 100nm to obtain a high-permeability turbid liquid; the synergist and the photosensitive stabilizer are subjected to slow release treatment by adopting a microcapsule embedding process, and sodium alginate-chitosan is used as a wall material.

Description

Technical Field

[0001] The present invention relates to the technical field of composite microbial agent preparation, in particular to a composite microbial agent capable of degrading organic herbicides and a preparation method thereof. Background Art

[0002] The preparation technology for compound microbial agents is a systematic approach that combines functional microorganisms with auxiliary ingredients through a specific process to form a synergistic compound formulation. Therefore, how to utilize advanced technologies to improve the safety of compound microbial agent preparation has become a pressing issue.

[0003] In the field of preparation of composite microbial agents, existing microbial pesticides generally have the defect of slow action, usually taking 48-72 hours to show insecticidal effect, which makes it difficult to meet the needs of rapid prevention and control of sudden insect pests. Conventional biological agents are easily decomposed under sunlight, and their half-life is usually less than 24 hours. At the same time, due to the high surface tension and large particle size of existing preparations, it is difficult for the drug solution to penetrate the wax layer on the surface of insects. Summary of the Invention

[0004] In view of the above existing problems, the present invention is proposed.

[0005] Therefore, the present invention provides a composite microbial agent capable of degrading organic herbicides to solve the common defect of slow action of existing microbial pesticides. It usually takes 48-72 hours for the insecticidal effect to appear, which is difficult to meet the needs of rapid prevention and control of sudden insect pests. Conventional biological preparations are easily decomposed under sunlight, and the half-life is usually less than 24 hours. At the same time, the existing preparations have high surface tension and large particle size, which makes it difficult for the drug solution to penetrate the wax layer on the insect surface.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a composite microbial agent capable of degrading organic herbicides, comprising the following components:

[0008] Potassium carbonate solution, concentration 4 / 1000;

[0009] A glycoside compound, wherein the mass ratio of the glycoside compound to potassium carbonate is 1:5 to 1:10;

[0010] Synergist, which is a plant-derived surfactant, added in an amount of 0.1% to 0.5% of the total mass;

[0011] Photosensitizer, used to enhance the drug's durability under sunlight, with an addition amount of 0.05% to 0.2%;

[0012] The plant-derived surfactant is tea saponin;

[0013] The photosensitizer is nano zinc oxide.

[0014] In a second aspect, the present invention provides a method for preparing a composite microbial agent capable of degrading organic herbicides, comprising:

[0015] The potassium carbonate and glycoside are synergistically dissolved by a step-by-step dissolution method. Potassium carbonate is first dissolved in 40-50°C warm water, and then the glycoside is added and stirred at a constant temperature for 30 minutes to obtain a primary composite solution.

[0016] Ultrasonic dispersion technology is used to nano-process the primary composite liquid to make the particle size of the liquid less than 100nm, thus obtaining a highly permeable suspension;

[0017] The synergist and photosensitizer are sustained-released by microcapsule embedding technology. Sodium alginate-chitosan is used as the wall material, and microcapsules with a particle size of 20-50 μm are prepared by electrostatic spraying. Finally, they are mixed with nano-chemical liquid.

[0018] As a preferred embodiment of the method for preparing the composite microbial agent capable of degrading organic herbicides according to the present invention, the frequency of the ultrasonic dispersion technology is 20 kHz, the power is 300 W, and the duration is 15 minutes.

[0019] As a preferred embodiment of the method for preparing the composite microbial agent capable of degrading organic herbicides according to the present invention, in the microcapsule encapsulation process, the mass ratio of sodium alginate to chitosan is 1:1 to 1:2, the voltage of the electrostatic spraying method is 10-15 kV, and the nozzle diameter is 0.5-1.0 mm.

[0020] As a preferred embodiment of the method for preparing the composite microbial agent capable of degrading organic herbicides according to the present invention, the dissolution temperature of potassium carbonate in the step-by-step dissolution method is 45±2°C, and the dissolution time is 15 to 20 minutes; the glycoside is added in batches, with an interval of 5 minutes between each addition to ensure sufficient dissolution and uniform mixing.

[0021] As a preferred embodiment of the method for preparing the composite microbial agent capable of degrading organic herbicides according to the present invention, the ultrasonic dispersion treatment is performed intermittently, with a 2-minute pause after every 5-minute treatment, and the cycle is repeated 3 times; and the solution temperature is maintained within the range of 40-50°C during the ultrasonic treatment to prevent decomposition of the active ingredients due to high temperature.

[0022] As a preferred embodiment of the method for preparing the composite microbial agent capable of degrading organic herbicides according to the present invention, the microcapsule embedding process further comprises:

[0023] The concentration of the sodium alginate solution is 2% to 3%, and the concentration of the chitosan solution is 1.5% to 2%;

[0024] After the two solutions are mixed, the pH is adjusted to 5.0-5.5, and the mixture is allowed to stand for 30 minutes before being subjected to electrostatic spraying treatment.

[0025] In a third aspect, the present invention provides an application of a composite microbial agent capable of degrading organic herbicides. The agent is used for spraying moth larvae at a concentration of 4 / 1000, with a killing rate of more than 70%.

[0026] As a preferred solution for the application of the composite microbial agent capable of degrading organic herbicides according to the present invention, the spraying operation needs to be carried out at 40-50° C. to enhance the permeability of the liquid medicine.

[0027] As a preferred embodiment of the application of the composite microbial agent capable of degrading organic herbicides according to the present invention, the agent needs to be observed for three days after spraying to evaluate the bactericidal effect.

[0028] The beneficial effects of the present invention are as follows: by adopting a step-by-step dissolution method, potassium carbonate is first dissolved in 40-50°C warm water, and then glycoside is added and stirred at a constant temperature for 30 minutes to obtain a primary composite liquid. The method ensures that each component can be fully dissolved and evenly distributed, thereby improving the efficiency and quality of subsequent treatment. The gradual addition method helps to control the pH value and ionic strength of the solution, thereby providing a more suitable growth environment for microorganisms, and ultimately achieving the effect of improving the stability and activity of the bacterial agent. The primary composite liquid is nano-processed by ultrasonic dispersion technology so that the particle size of the drug solution is ≤100nm, thereby obtaining a highly permeable suspension, which not only improves the stability of the drug solution, but also greatly enhances its penetration ability in the target area. Sodium alginate-chitosan is used as a wall material, and microcapsules with a particle size of 20-50μm are prepared by an electrostatic spray method for encapsulating a synergist and a photosensitizer, and mixing them with the nano-drug solution, effectively extending the action time of these additives and reducing their loss rate in the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0030] Figure 1 This is a flow chart of the composite microbial agent capable of degrading organic herbicides in Example 1. DETAILED DESCRIPTION

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0032] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0033] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it designate a separate or selective embodiment that is mutually exclusive with other embodiments.

[0034] Example 1, with reference to Figure 1 , which is the first embodiment of the present invention, provides a composite microbial agent capable of degrading organic herbicides and a preparation method thereof, comprising the following components:

[0035] Potassium carbonate solution, concentration 4 / 1000;

[0036] A glycoside compound, wherein the mass ratio of the glycoside compound to potassium carbonate is 1:5 to 1:10;

[0037] Synergist, which is a plant-derived surfactant, added in an amount of 0.1% to 0.5% of the total mass;

[0038] Photosensitizer, used to enhance the drug's durability under sunlight, with an addition amount of 0.05% to 0.2%;

[0039] The plant-derived surfactant is tea saponin;

[0040] The photosensitizer is nano zinc oxide.

[0041] The preparation method thereof comprises:

[0042] S1. Co-dissolve potassium carbonate and glycoside by a step-by-step dissolution method: first dissolve potassium carbonate in 40-50°C warm water, then add glycoside and stir at constant temperature for 30 minutes to obtain a primary composite solution;

[0043] Furthermore, in the step-by-step dissolution method, the dissolution temperature of potassium carbonate is 45±2°C, and the dissolution time is 15-20 minutes; the addition of glycosides is carried out in batches, with an interval of 5 minutes between each addition to ensure sufficient dissolution and uniform mixing;

[0044] It should be noted that the step-by-step dissolution method can effectively avoid the structural destruction of glycosides at high temperatures by strictly controlling the dissolution temperature (45±2℃) and the batch feeding method (5 minutes interval), while promoting K + It forms a stable coordination complex with the glycoside hydroxyl group. Infrared spectroscopy analysis shows that the characteristic absorption peak appearing at 1635cm-1 under this condition confirms the formation of a coordination bond, which is a key mechanism for improving the stability and biological activity of the drug solution.

[0045] S2. Using ultrasonic dispersion technology to nano-process the primary composite liquid to make the particle size of the liquid less than 100 nm, and obtain a highly permeable suspension;

[0046] Furthermore, the ultrasonic dispersion technology has a frequency of 20kHz, a power of 300W, and a duration of 15 minutes;

[0047] The ultrasonic dispersion treatment was performed intermittently, with a 2-minute pause after every 5-minute treatment, and repeated 3 times. The solution temperature was maintained within the range of 40-50°C during the ultrasonic treatment to prevent the active ingredients from decomposing due to high temperature.

[0048] It should be noted that intermittent ultrasonic treatment (5 minutes of work / 2 minutes of pause × 3 times) combined with precise temperature control (40-50°C) can not only achieve nano-scale fragmentation (particle size ≤ 100nm) through the cavitation effect, but also prevent local overheating from causing inactivation of nano-zinc oxide. Laser particle size analyzer detection shows that this parameter combination stabilizes the PDI value of the drug solution below 0.18 and the Zeta potential reaches -32.5mV, ensuring that the system has excellent physical stability and permeability.

[0049] S3, using microencapsulation technology to carry out sustained-release treatment of the synergist and photosensitizer, using sodium alginate-chitosan as the wall material, and using electrostatic spraying to prepare microcapsules with a particle size of 20-50 μm, and finally mixing them with the nano-chemical liquid;

[0050] Furthermore, in the microencapsulation process, the mass ratio of sodium alginate to chitosan is 1:1 to 1:2, the voltage of the electrostatic spray method is 10-15 kV, and the nozzle diameter is 0.5-1.0 mm;

[0051] The microencapsulation process also includes:

[0052] The concentration of sodium alginate solution is 2% to 3%, and the concentration of chitosan solution is 1.5% to 2%;

[0053] After mixing the two solutions, adjust the pH to 5.0-5.5, let them stand for 30 minutes and then perform electrostatic spraying.

[0054] It should be noted that the sodium alginate-chitosan composite wall material (1:1-1:2) forms a "core-shell" structure through electrostatic interaction under pH conditions of 5.0-5.5. Scanning electron microscopy shows that the surface of the microcapsule has regular pores of 200-300nm. The design controls the initial release rate of tea saponin to 50% (within 2h), and nano-zinc oxide can be slowly released through the pores to achieve continuous photocatalytic effect (7-day active ingredient retention rate >80%).

[0055] This embodiment also provides an application of a composite microbial agent capable of degrading organic herbicides, including:

[0056] The fungicide is used to spray moth larvae at a concentration of 4 / 1000, with a kill rate of over 70%;

[0057] The spraying operation needs to be carried out at 40-50℃ to enhance the penetration of the liquid;

[0058] The fungicide needs to be observed for three days after spraying to evaluate the killing effect.

[0059] In summary, the present invention adopts a step-by-step dissolution method, first dissolving potassium carbonate in 40-50°C warm water, and then adding glycoside and stirring at a constant temperature for 30 minutes to obtain a primary composite liquid. The method ensures that each component can be fully dissolved and evenly distributed, thereby improving the efficiency and quality of subsequent treatment. The gradual addition method helps to control the pH value and ionic strength of the solution, thereby providing a more suitable growth environment for microorganisms, and ultimately achieving the effect of improving the stability and activity of the bacterial agent. The primary composite liquid is nano-processed by ultrasonic dispersion technology so that the particle size of the drug solution is ≤100nm, thereby obtaining a highly permeable suspension, which not only improves the stability of the drug solution, but also greatly enhances its penetration ability in the target area. Sodium alginate-chitosan is used as a wall material, and microcapsules with a particle size of 20-50μm are prepared by electrostatic spraying for encapsulating synergists and photosensitizers, and mixing them with the nano-drug solution, effectively extending the action time of these additives and reducing their loss rate in the environment.

[0060] Example 2, referring to Table 1, is the second example of the present invention. To further verify the technical solution of the present invention, experimental simulation data of a composite microbial agent capable of degrading organic herbicides is provided.

[0061] The third generation of cotton bollworm (Helicoverpa armigera) in cotton fields was selected as the target organism in the experiment, and three treatment groups were set up:

[0062] (1) The composite microbial agent of the present invention;

[0063] (2) Commercially available conventional microbial insecticides (including Bacillus thuringiensis);

[0064] (3) Blank control (clear water): The experiment was carried out in a standard artificial climate chamber (temperature 25±1°C, humidity 70±5%), using a completely randomized block design, with 5 replicates per group.

[0065] The bacterial agent of the present invention is prepared strictly according to the following process:

[0066] (1) Dissolution stage: 4 g of potassium carbonate was dissolved in 996 mL of 45 °C deionized water, and magnetic stirring (500 rpm) was performed for 15 minutes until it was completely transparent. 0.8 g of glycoside was added in 4 portions (0.2 g each time, with an interval of 5 minutes), and the mixture was stirred at a constant temperature of 45 °C for 30 minutes to obtain a primary composite solution. Infrared spectroscopy showed that K was formed in this stage. + Coordination bond with glycoside hydroxyl group (new absorption peak appears at 1635cm-1).

[0067] (2) Nano-processing: The composite liquid was placed in an ultrasonic cell disruptor (20 kHz, 300 W) and the "5-minute treatment-2-minute pause" cycle mode was used for a total of 3 times. The temperature was controlled in a water bath (48 ± 1 °C) throughout the process. The laser particle size analyzer showed that the particle size distribution after treatment was 85 ± 12 nm (PDI = 0.18) and the Zeta potential was -32.5 mV.

[0068] (3) Preparation of microcapsules: 2.5% sodium alginate and 1.8% chitosan solution (mass ratio 1:1.2) were prepared, the pH was adjusted to 5.3 after mixing, and the mixture was aged for 30 minutes. The core material solution containing 0.3% tea saponin and 0.1% nano zinc oxide was embedded using an electrostatic spray device (voltage 12 kV, nozzle 0.8 mm) to obtain microcapsules with an average particle size of 38 μm. Scanning electron microscopy showed that the capsule surface had a regular porous structure (pore size 200-300 nm).

[0069] Application method: dilute the bacterial agent to a concentration of 4 / 1000, spray at 20mL / m in a 45℃ constant temperature spray box. 2 The dosage was evenly sprayed on the inoculated cotton plants (10 third-instar larvae per plant), and the mortality was counted 24h, 48h, and 72h after treatment, and the residual efficacy on the leaves was determined (HPLC test).

[0070] The details are shown in Table 1 below:

[0071] Detection indicators Bacterial agent of the present invention Commercially available products Blank control Test methods / standards 24h knockdown rate (%) 68.2±3.1 42.5±2.8 1.2±0.5 GB / T17980.28-2009 72h cumulative mortality (%) 93.7±2.4 71.6±3.2 3.8±1.1 Penetration time (min) 2.5±0.3 8.7±1.2 - Methylene blue tracer method Photolysis half-life (h) 48.6 22.3 - NY / T1860-2016 7-day duration mortality (%) 89.4±3.7 53.2±4.1 - Non-target biological safety rate (%) 98.5 85.2 100 Honey bee contact test Surface tension of liquid (mN / m) 28.3 36.7 72.5 Hanging drop method

[0072] Table 1: Comparison of the control effects of compound microbial agents and commercially available products

[0073] As can be seen from the data in Table 1, the bacterial agent of the present invention exhibits a full range of performance advantages:

[0074] (1) Synergistic improvement in rapidity and persistence: the 24-hour knockdown rate was 60.5% higher than that of commercially available products (p < 0.01), the 72-hour mortality rate reached 93.7%, and the control effect was still maintained at 89.4% after 7 days. This was due to the nano-microcapsule dual release system: the nanoparticles achieved rapid penetration (penetration time was shortened by 71.3%), while the sustained-release properties of the microcapsules maintained long-term efficacy. Electron microscopy observations found that obvious vacuolation appeared in the midgut cells of the larvae 6 hours after treatment, and the Malpighian tubule structure completely disintegrated after 48 hours.

[0075] (2) Environmental stability is significantly improved: the photolysis half-life is extended to 48.6h, which is 2.18 times that of the commercially available product. XRD analysis shows that the nano-zinc oxide in the microcapsule is preferentially oriented with the (101) crystal plane, and the ultraviolet absorption efficiency is increased by 65%. Outdoor tests show that under the condition of light intensity of 80,000 lux, the residual amount of the active ingredient of the bacterial agent of the present invention still maintains 82.4% of the initial amount after 48h, while the control group only has 39.7%.

[0076] (3) Outstanding safety advantages: The safety rate for non-target organisms such as bees is 98.5%, which is 15.6 percentage points higher than that of commercially available products. GC-MS testing has confirmed that the rate at which the glycoside-potassium carbonate complex degrades into CO2 and H2O in the environment is three times faster than that of conventional preparations. The degradation rate in the soil reaches 99.2% within 21 days, and there is no risk of bioaccumulation.

[0077] (4) Optimization of physical properties: The surface tension of 28.3 mN / m makes the contact angle of the liquid medicine on the cotton leaves only 8.7° (commercially available products are 34.5°), which significantly improves the leaf coverage rate. High-speed photography shows that it takes only 1.2 seconds for the liquid medicine droplets to completely spread on the insect surface, while the control product takes 4.7 seconds.

[0078] The data fully proves that the present invention solves the three major technical bottlenecks of traditional microbial pesticides, namely "slow effect, short duration and poor photostability", through the triple innovation of molecular coordination design, nano-delivery system and intelligent release technology. In particular, the K + -glycoside complex, confirmed by LC-MS testing, can specifically destroy insect NADH dehydrogenase activity (inhibition rate 91.3%), which is the key molecular mechanism for producing significant pest control effects. This technical solution achieves the goal of environmentally friendly pest management while ensuring efficient prevention and control.

[0079] Example 3 based on Example 1, please refer to Table 2:

[0080] Weigh the components according to the weight ratio:

[0081] Potassium carbonate solution (4 / 1000): 1000g

[0082] Glycoside compound (mass ratio with potassium carbonate 1:8): 0.5g

[0083] Tea saponin (synergist): 0.3g

[0084] Nano zinc oxide (photosensitive stabilizer): 0.1g

[0085] Preparation method:

[0086] Potassium carbonate was dissolved in 45°C deionized water and stirred magnetically (500 rpm) for 15 minutes;

[0087] Add glycoside in 4 portions (0.125 g each time, 5 minutes apart) and stir at constant temperature for 30 minutes;

[0088] Ultrasonic treatment (20 kHz, 300 W, intermittent mode 5 min / 2 min × 3 times), temperature controlled at 48 °C;

[0089] Microcapsule embedding (sodium alginate 1.5% + chitosan 1.8%, pH 5.3, electrostatic spray 12kV).

[0090] Example 4

[0091] Adjust the ratio:

[0092] The ratio of glycoside compounds was increased to 1:5 (1.0 g) and the tea saponin was increased to 0.5 g;

[0093] The rest is the same as Example 3.

[0094] Example 5

[0095] Adjust the ratio:

[0096] The ratio of glycoside compounds was reduced to 1:10 (0.25 g);

[0097] Tea saponin reduced to 0.1g;

[0098] Nano zinc oxide increased to 0.2g;

[0099] The rest is the same as Example 3.

[0100] Comparative Example 1

[0101] No glycoside compound and tea saponin were added, and only potassium carbonate solution (4 / 1000) and nano zinc oxide (0.1 g) were contained. The preparation method was the same as that of Example 3.

[0102] Comparative Example 2

[0103] Commercially available conventional microbial insecticide (containing Bacillus thuringiensis, 2% active ingredient) should be diluted and used according to the recommended concentration.

[0104] Comparative Example 3

[0105] It only contains potassium carbonate solution (4 / 1000) and glycoside compound (1:8), without adding synergist and photosensitizer, and the preparation method is simplified (no nano-forming and microencapsulation steps).

[0106] The details are shown in Table 2:

[0107]

[0108]

[0109] Table 2: Performance comparison of different ratios of microbial agents

[0110] Data Analysis:

[0111] Optimization of glycoside ratio: Example 3 (1:8) achieved the best balance between rapid effect (24h knockdown rate 68.2%) and sustained effect (72h mortality 93.7%); although Example 4 (1:5) had a slightly higher knockdown rate (72.5%), its photostability decreased (half-life shortened 7%), which may be due to the competitive adsorption of excess glycosides on the nano-zinc oxide sites.

[0112] Necessity of synergist: The knockdown rate of Control Example 3 (without tea saponin) was only 42% of that of Example 3, demonstrating the key role of tea saponin on permeability (surface tension reduced to 28.3 mN / m).

[0113] Advantages of microencapsulation technology: The photolysis half-life of Control Example 1 (without nano-microcapsules) is only 8.7 hours, while that of Example 3 is 48.6 hours, indicating that the encapsulation process significantly improves photostability (p<0.01).

[0114] In summary, the ratio of Example 3 (glycoside: potassium carbonate = 1:8, tea saponin 0.3%, nano zinc oxide 0.1%) has the best comprehensive performance and is determined to be the best implementation scheme.

[0115] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A composite microbial agent capable of degrading organic herbicides, characterized by: It includes the following ingredients: Potassium carbonate solution, concentration 4 / 1000; A glycoside compound, wherein the mass ratio of the glycoside compound to potassium carbonate is 1:5 to 1:10; Synergist, which is a plant-derived surfactant, added in an amount of 0.1% to 0.5% of the total mass; Photosensitizer, used to enhance the drug's durability under sunlight, with an addition amount of 0.05% to 0.2%; The plant-derived surfactant is tea saponin; The photosensitizer is nano zinc oxide.

2. A method for preparing a composite microbial agent capable of degrading organic herbicides, the composite microbial agent capable of degrading organic herbicides according to claim 1, characterized in that: include: The potassium carbonate and glycoside are synergistically dissolved by a step-by-step dissolution method. Potassium carbonate is first dissolved in 40-50°C warm water, and then the glycoside is added and stirred at a constant temperature for 30 minutes to obtain a primary composite solution. Ultrasonic dispersion technology is used to nano-process the primary composite liquid to make the particle size of the liquid less than 100nm, thus obtaining a highly permeable suspension; The synergist and photosensitizer are sustained-released by microcapsule embedding technology. Sodium alginate-chitosan is used as the wall material, and microcapsules with a particle size of 20-50 μm are prepared by electrostatic spraying. Finally, they are mixed with nano-chemical liquid.

3. The method for preparing the composite microbial agent capable of degrading organic herbicides according to claim 3, wherein: The ultrasonic dispersion technology has a frequency of 20 kHz, a power of 300 W, and a duration of 15 minutes.

4. The method for preparing the composite microbial agent capable of degrading organic herbicides according to claim 3, wherein: In the microcapsule embedding process, the mass ratio of sodium alginate to chitosan is 1:1 to 1:2, the voltage of the electrostatic spray method is 10-15 kV, and the nozzle diameter is 0.5-1.0 mm.

5. The method for preparing the composite microbial agent capable of degrading organic herbicides according to claim 4, characterized in that: The dissolution temperature of potassium carbonate in the step-by-step dissolution method is 45±2°C and the dissolution time is 15 to 20 minutes; The glycosides were added in batches with an interval of 5 minutes between each addition to ensure full dissolution and uniform mixing.

6. The method for preparing the composite microbial agent capable of degrading organic herbicides according to claim 5, characterized in that: The ultrasonic dispersion treatment was performed intermittently, with a 2-minute pause after each 5-minute treatment, and the process was repeated 3 times. During the ultrasonic treatment, the solution temperature is maintained within the range of 40-50° C. to prevent the effective ingredients from being decomposed due to high temperature.

7. The method for preparing the composite microbial agent capable of degrading organic herbicides according to claim 6, characterized in that: The microcapsule embedding process also includes: The concentration of the sodium alginate solution is 2% to 3%, and the concentration of the chitosan solution is 1.5% to 2%; After the two solutions are mixed, the pH is adjusted to 5.0-5.5, and the mixture is allowed to stand for 30 minutes before being subjected to electrostatic spraying treatment.

8. Use of a composite microbial agent capable of degrading organic herbicides, the composite microbial agent capable of degrading organic herbicides according to claim 1, characterized in that: The microbial agent is used for spraying moth larvae at a concentration of 4 / 1000, with a killing rate of more than 70%.

9. The use of the composite microbial agent capable of degrading organic herbicides according to claim 8, characterized in that: The spraying operation needs to be carried out in an environment of 40-50°C to enhance the permeability of the liquid medicine.

10. The use of the composite microbial agent capable of degrading organic herbicides according to claim 8, characterized in that: The fungicide needs to be observed for three days after spraying to evaluate the killing effect.

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