Application of composition for preventing and treating tobacco wildfire disease in enhancing resistance of tobacco to tobacco wildfire disease

Abstract

The invention provides application of a composition for preventing and treating tobacco wildfire disease in enhancing resistance of tobacco to tobacco wildfire disease. The composition comprises nano titanium dioxide, nano copper hydroxide and nano silver. The invention creatively finds that the nano titanium dioxide, the nano copper hydroxide and the nano silver have a synergistic effect in the aspect of enhancing the resistance of the tobacco to the tobacco wildfire germs, so that the tobacco wildfire germs can be obviously inhibited, the tobacco wildfire disease is prevented and treated, the incidence rate of the tobacco wildfire disease is further reduced, the quality and the yield of the tobacco are improved, and meanwhile, the effect of preventing and treating the tobacco wildfire disease is achieved. Scab generation and amplification in the period from harvesting to drying of the tobacco leaves can be avoided, and the utilization rate of the tobacco leaves is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of tobacco disease prevention and control, and particularly relates to an application of a composition for preventing and controlling tobacco wildfire disease in enhancing the resistance of tobacco to tobacco wildfire disease bacteria. Background Art

[0002] Tobacco wildfire, commonly known as "burn disease," is caused by the Gram-negative bacteria Pseudomonas tabaci (Wolf et Foster) Stevens. It is a common bacterial disease in tobacco fields, severely impacting tobacco yields. It primarily attacks leaves, but can also affect young stems, capsules, and sepals. Initially, small, brown, water-soaked spots appear on leaves, surrounded by a wide yellow halo. These spots gradually expand, reaching 1-2 cm in diameter. They coalesce to form large, irregular spots with irregular whorls.

[0003] Tobacco wildfire is found in most tobacco-growing areas worldwide and in all tobacco-growing areas of China. It is most severely affected in northern tobacco-growing areas, while in southern tobacco-growing areas it is only particularly severe in sun-cured tobacco and only occurs sporadically in flue-cured tobacco. Tobacco wildfire not only harms tobacco leaves in the field but also continues to harm them after harvest and before they are dried. The diseased parts become charred, and the lesions continue to expand, rendering them useless. The main control methods for tobacco wildfire include chemical, biological, and agricultural control, with chemical control being the most important. However, there is currently a lack of effective chemical control agents for tobacco wildfire, resulting in severe disease in tobacco fields and a significant reduction in tobacco quality and yield. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a composition for preventing and treating tobacco wildfire disease for use in enhancing the resistance of tobacco to tobacco wildfire disease bacteria. The present invention creatively discovered that nano-titanium dioxide, nano-copper hydroxide and nano-silver have excellent prevention and treatment effects on tobacco wildfire disease, significantly reducing the incidence of diseases in tobacco fields and improving the quality and yield of tobacco.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides an application of a composition for preventing and treating tobacco wildfire disease in enhancing tobacco's resistance to tobacco wildfire disease bacteria, wherein the composition comprises nano-titanium dioxide, nano-copper hydroxide and nano-silver.

[0007] The present invention creatively discovered that nano-titanium dioxide, nano-copper hydroxide and nano-silver have a synergistic effect in enhancing tobacco's resistance to tobacco wildfire bacteria. They can significantly inhibit tobacco wildfire bacteria, prevent and control tobacco wildfire disease, thereby reducing the incidence of tobacco wildfire disease and improving tobacco quality and yield. At the same time, it can avoid the generation and expansion of lesions on tobacco leaves from harvest to drying, thereby improving the utilization rate of tobacco leaves.

[0008] Preferably, the composition comprises 24-46 parts of nano titanium dioxide, 18-38 parts of nano copper hydroxide and 10-26 parts of nano silver in parts by weight.

[0009] The weight proportions of the nano titanium dioxide can be 24 parts, 26 parts, 28 parts, 30 parts, 32 parts, 34 parts, 36 parts, 38 parts, 40 parts, 42 parts, 44 parts or 46 parts.

[0010] The weight proportions of the nano copper hydroxide may be 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, 32 parts, 34 parts, 36 parts or 38 parts.

[0011] The weight proportions of the nanosilver can be 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts or 26 parts.

[0012] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0013] The nano-titanium dioxide, nano-copper hydroxide and nano-silver in the present invention are combined in the above-mentioned specific content ratio, which has a synergistic effect in enhancing the resistance of tobacco to tobacco wildfire bacteria, has a good inhibitory effect on tobacco wildfire bacteria, and has a good preventive and control effect on tobacco wildfire disease.

[0014] Preferably, the composition comprises 30-40 parts of nano titanium dioxide, 22-30 parts of nano copper hydroxide and 15-20 parts of nano silver in parts by weight.

[0015] The weight proportions of the nano-titanium dioxide may be 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts or 40 parts.

[0016] The weight proportions of the nano copper hydroxide may be 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts or 30 parts.

[0017] The weight proportions of the nanosilver may be 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19.5 parts or 20 parts, etc.

[0018] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0019] Furthermore, the nano-titanium dioxide, nano-copper hydroxide and nano-silver in the present invention have the best inhibitory effect on tobacco wildfire bacteria under the above-mentioned specific content ratio, and have the best prevention and control effect on tobacco wildfire disease.

[0020] Preferably, the particle size of the nano-titanium dioxide, nano-copper hydroxide and nano-silver is independently 1-100 nm, for example, 1 nm, 10 nm, 20 nm, 30 nm, 40 nm, 50 nm, 60 nm, 70 nm, 80 nm, 90 nm or 100 nm.

[0021] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0022] In a second aspect, the present invention provides a preparation for preventing and treating tobacco wildfire, comprising a composition for preventing and treating tobacco wildfire and an agriculturally acceptable functional adjuvant; the composition comprises, by weight, 24-46 parts of nano-titanium dioxide, 18-38 parts of nano-copper hydroxide, and 10-26 parts of nano-silver.

[0023] The weight proportions of the nano titanium dioxide can be 24 parts, 26 parts, 28 parts, 30 parts, 32 parts, 34 parts, 36 parts, 38 parts, 40 parts, 42 parts, 44 parts or 46 parts.

[0024] The weight proportions of the nano copper hydroxide may be 18 parts, 20 parts, 22 parts, 24 parts, 26 parts, 28 parts, 30 parts, 32 parts, 34 parts, 36 parts or 38 parts.

[0025] The weight proportions of the nanosilver can be 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 parts, 24 parts or 26 parts.

[0026] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0027] The present invention creatively discovered that the combination of nano-titanium dioxide, nano-copper hydroxide and nano-silver in the above-mentioned specific content ratio has a synergistic effect in enhancing the resistance of tobacco to tobacco wildfire bacteria, can significantly inhibit tobacco wildfire bacteria, prevent and control tobacco wildfire disease, thereby reducing the incidence of tobacco wildfire disease, and improving the quality and yield of tobacco. At the same time, it can avoid the generation and expansion of lesions on tobacco leaves from harvest to drying, thereby improving the utilization rate of tobacco leaves.

[0028] Preferably, the composition comprises 30-40 parts of nano titanium dioxide, 22-30 parts of nano copper hydroxide and 15-20 parts of nano silver in parts by weight.

[0029] The weight proportions of the nano-titanium dioxide may be 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts or 40 parts.

[0030] The weight proportions of the nano copper hydroxide may be 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts or 30 parts.

[0031] The weight proportions of the nanosilver may be 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19.5 parts or 20 parts, etc.

[0032] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0033] Furthermore, the nano-titanium dioxide, nano-copper hydroxide and nano-silver in the present invention have the best inhibitory effect on tobacco wildfire bacteria under the above-mentioned specific content ratio, and have the best prevention and control effect on tobacco wildfire disease.

[0034] Preferably, the mass percentage of the composition in the preparation is 5-90%, for example, it can be 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% or 90%.

[0035] Preferably, the mass percentage of the composition in the preparation is 13-85%, for example, it can be 13%, 15%, 25%, 35%, 45%, 55%, 65%, 75% or 85%.

[0036] Other specific point values within the above numerical ranges can be selected and will not be described in detail here.

[0037] Preferably, the agriculturally acceptable functional adjuvant includes any one or a combination of at least two of a solvent, an emulsifier, a dispersant, a wetting agent, an adhesive, a stabilizer, a defoaming agent, a diluent, a thickener or an antifreeze agent.

[0038] Preferably, the dosage form of the preparation includes any one of powder, suspension, microemulsion, ointment, plaster or spread.

[0039] Preferably, the formulation is applied by any one of foliar spraying, soil mixing, root irrigation, seed soaking, furrow application or hole application, or a combination of at least two of them.

[0040] In a third aspect, the present invention provides a method for controlling tobacco wildfire, comprising applying the formulation of the second aspect to tobacco and / or its cultivation medium before or after the tobacco is infected.

[0041] Preferably, nano-titanium dioxide, nano-copper hydroxide and nano-silver are applied simultaneously or sequentially.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] The present invention creatively discovered that nano-titanium dioxide, nano-copper hydroxide and nano-silver have a synergistic effect in enhancing tobacco's resistance to tobacco wildfire bacteria. They can significantly inhibit tobacco wildfire bacteria, prevent and control tobacco wildfire disease, and thus reduce the incidence of tobacco wildfire disease, improve tobacco quality and yield, and at the same time, avoid the generation and expansion of lesions on tobacco leaves from harvest to drying, thereby improving the utilization rate of tobacco leaves; the ingredients are safe and do not pollute the soil environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 Pictures of tobacco leaf lesions on tobacco K326 leaves inoculated with the composition prepared in Example 1 or Comparative Examples 1-3 and then with tobacco syringa syringa. DETAILED DESCRIPTION

[0045] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0046] If no specific techniques or conditions are specified in the examples, the experiments were carried out according to the techniques or conditions described in the literature in the field or according to the product instructions. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0047] The sources of materials used in the following specific embodiments are as follows:

[0048] Raw material name Purchase manufacturer model Nano-titanium dioxide Solarbio NM000800 Nano copper hydroxide Shanghai Chengxin Industrial Co., Ltd. 20427-59-2 Nanosilver Solarbio NM000620 Copper hydroxide Acmec C36540-500g Nano copper oxide Acmec C49342-25g Nano zinc oxide Acmec Z86341-100g Nano-alumina Acmec A26796-100g Nano-cobalt oxide Aladdin C111615-5g Nano-cerium oxide Aladdin C103984-500g

[0049] Example 1

[0050] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 35 parts of nano-titanium dioxide, 25 parts of nano-copper hydroxide, and 18 parts of nano-silver. The preparation method is to uniformly mix the above components.

[0051] Example 2

[0052] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 30 parts of nano-titanium dioxide, 30 parts of nano-copper hydroxide, and 20 parts of nano-silver. The preparation method is to mix the above components evenly.

[0053] Example 3

[0054] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 40 parts of nano-titanium dioxide, 22 parts of nano-copper hydroxide, and 15 parts of nano-silver. The preparation method is to mix the above components evenly.

[0055] Example 4

[0056] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 24 parts of nano-titanium dioxide, 38 parts of nano-copper hydroxide, and 26 parts of nano-silver. The preparation method is to uniformly mix the above components.

[0057] Example 5

[0058] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 46 parts of nano-titanium dioxide, 18 parts of nano-copper hydroxide, and 10 parts of nano-silver. The preparation method is to uniformly mix the above components.

[0059] Example 6

[0060] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 6 parts of nano-titanium dioxide, 42 parts of nano-copper hydroxide, and 30 parts of nano-silver. The preparation method is to mix the above components evenly.

[0061] Example 7

[0062] This embodiment provides a composition for preventing and treating tobacco wildfire, which comprises, by weight, 55 parts of nano-titanium dioxide, 15 parts of nano-copper hydroxide, and 8 parts of nano-silver. The preparation method is to uniformly mix the above components.

[0063] Comparative Example 1

[0064] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano-titanium dioxide is not added, and its reduced amount is proportionally distributed to nano-copper hydroxide and nano-silver.

[0065] Comparative Example 2

[0066] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano-copper hydroxide is not added, and its reduced amount is proportionally distributed to nano-titanium dioxide and nano-silver.

[0067] Comparative Example 3

[0068] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano-silver is not added, and its reduced amount is proportionally distributed to nano-titanium dioxide and nano-copper hydroxide.

[0069] Comparative Example 4

[0070] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano copper hydroxide is replaced with an equal portion of copper hydroxide.

[0071] Comparative Example 5

[0072] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano-copper hydroxide is replaced by an equal portion of nano-copper oxide.

[0073] Comparative Example 6

[0074] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano copper hydroxide is replaced by an equal portion of nano zinc oxide.

[0075] Comparative Example 7

[0076] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano copper hydroxide is replaced by an equal portion of nano aluminum oxide.

[0077] Comparative Example 8

[0078] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano-titanium dioxide is replaced by an equal portion of nano-cobalt oxide.

[0079] Comparative Example 9

[0080] This comparative example provides a composition for preventing and treating tobacco wildfire, which differs from Example 1 only in that nano-titanium dioxide is replaced by an equal portion of nano-cerium oxide.

[0081] Comparative Example 10

[0082] This comparative example provides a commercially available tobacco fungicide.

[0083] Test Example 1

[0084] Pseudomonas syringae pv. tabaci (kindly provided by Zhengzhou Tobacco Science Research Institute) was cultured in LB liquid medium with shaking at 28°C overnight. The bacterial solution was centrifuged at 5000 rpm for 10 min, and the bacterial pellet was resuspended in PBS solution for washing. The pellet was centrifuged again and resuspended in PBS solution to prepare a Pseudomonas syringae pv. tabaci suspension (1×10 5 CFU / mL). Take 200 μL of tobacco wildfire bacteria suspension and add the compositions provided in Examples 1-7, Comparative Examples 1-9 or the preparation provided in Comparative Example 10 (final concentration 9 ppb), respectively. At the same time, single nano-titanium dioxide, nano-copper hydroxide and nano-silver are used for comparison. The culture is shaken at 28°C for 12 hours. The OD values of tobacco wildfire bacteria in different treatment groups before and after culture are calculated. 600 The inhibitory effect on tobacco syringa was determined by the change value of

[0085] The test results are shown in Table 1. Nano-titanium dioxide, nano-copper hydroxide and nano-silver have a synergistic effect in enhancing tobacco's resistance to tobacco wildfire bacteria. The three have a significant inhibitory effect on tobacco wildfire bacteria. The three have excellent synergistic effects only within a specific ratio range. Nano-titanium dioxide, nano-copper hydroxide and nano-silver are irreplaceable compared to other metal nanoparticles. Not any combination of metal nanoparticles can significantly inhibit tobacco wildfire bacteria.

[0086] Table 1

[0087]

[0088]

[0089] Test Example 2

[0090] Tobacco K326 seeds were sown on nutrient soil and transplanted into individual pots after germination, when two to four true leaves appeared. When the seeds reached the six-leaf, one-heart stage, an inoculation experiment with tobacco syringa was conducted. One day before inoculation, the tobacco plants were sprayed with 10 mL of 9 ppb of the compositions provided in Examples 1-7 and Comparative Examples 1-9, or the formulation provided in Comparative Example 10. Nano-titanium dioxide, nano-copper hydroxide, and nano-silver were also used for comparison. A blank control group was not treated before inoculation.

[0091] Pseudomonas syringae pv. tabaci (kindly provided by Zhengzhou Tobacco Science Research Institute) was cultured in LB liquid medium with shaking at 28°C overnight. The bacterial solution was centrifuged at 5000 rpm for 10 min, and the bacterial pellet was resuspended in PBS solution for washing. The pellet was centrifuged again and resuspended in PBS solution to prepare a Pseudomonas syringae pv. tabaci suspension (1×10 5 CFU / mL).

[0092] Puncture the leaf with a 1mL syringe needle. Press the upper surface of the leaf with one hand at the small hole and inject 200μL of bacterial solution from the lower surface of the leaf. Inoculate 10 tobacco plants in each group. Inoculate on the two fully expanded leaves at the top of each tobacco plant. The inoculation position is random. 1-10 days after inoculation, observe and record the number of lesions on the leaves. If necrotic spots appear on the leaves and there is a surrounding yellow halo, the leaves are judged to be infected if typical symptoms of tobacco wildfire appear. Take pictures on the 10th day and count the area of lesions ( Figure 1 ).

[0093] The test results are shown in Table 2. Nano-titanium dioxide, nano-copper hydroxide and nano-silver have a synergistic effect in enhancing tobacco's resistance to tobacco wildfire bacteria. The three work together to have a significant preventive and therapeutic effect on tobacco wildfire. The three have excellent synergistic effects only within a specific ratio range. Nano-titanium dioxide, nano-copper hydroxide and nano-silver are irreplaceable compared to other metal nanoparticles. Not any combination of metal nanoparticles can significantly prevent and treat tobacco wildfire.

[0094] Table 2

[0095]

[0096]

[0097] The present invention uses the above-described embodiments to illustrate the use of a composition for preventing and treating tobacco wildfire in enhancing tobacco resistance to tobacco wildfire pathogens. However, the present invention is not limited to the above-described embodiments, and does not necessarily rely on the above-described embodiments for implementation. Those skilled in the art will appreciate that any improvements to the present invention, equivalent replacements for various raw materials in the product of the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0098] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0099] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition for preventing and treating tobacco wildfire disease in enhancing tobacco wildfire disease resistance, characterized in that: The composition comprises nano titanium dioxide, nano copper hydroxide and nano silver.

2. The use according to claim 1, characterized in that The composition comprises 24-46 parts of nano titanium dioxide, 18-38 parts of nano copper hydroxide and 10-26 parts of nano silver in parts by weight.

3. The use according to claim 1 or 2, characterized in that The composition comprises 30-40 parts of nano titanium dioxide, 22-30 parts of nano copper hydroxide and 15-20 parts of nano silver in parts by weight.

4. A preparation for preventing and treating tobacco wildfire, characterized in that: The preparation comprises a composition for preventing and treating tobacco wildfire and an agriculturally acceptable functional adjuvant; the composition comprises 24-46 parts of nano titanium dioxide, 18-38 parts of nano copper hydroxide and 10-26 parts of nano silver in parts by weight.

5. The preparation according to claim 4, characterized in that The composition comprises 30-40 parts of nano titanium dioxide, 22-30 parts of nano copper hydroxide and 15-20 parts of nano silver in parts by weight.

6. The preparation according to claim 4 or 5, characterized in that The mass percentage of the composition in the preparation is 5-90%; Preferably, the mass percentage of the composition in the preparation is 13-85%.

7. The preparation according to any one of claims 4 to 6, characterized in that The agriculturally acceptable functional adjuvant includes any one or a combination of at least two of solvents, emulsifiers, dispersants, wetting agents, adhesives, stabilizers, defoaming agents, diluents, thickeners or antifreeze agents.

8. The preparation according to any one of claims 4 to 7, characterized in that The dosage form of the preparation includes any one of powder, suspension, microemulsion, ointment, plaster or spread; Preferably, the formulation is applied by any one of foliar spraying, soil mixing, root irrigation, seed soaking, furrow application or hole application, or a combination of at least two of them.

9. A method for preventing and controlling tobacco wildfire, characterized in that: The formulation according to any one of claims 4 to 8 is applied to the tobacco and / or its cultivation medium before or after the tobacco is infected.

10. The method according to claim 9, characterized in that Nano titanium dioxide, nano copper hydroxide and nano silver are applied simultaneously or sequentially.

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