Probiotic fermentation-based feed for field snails and preparation method of probiotic fermentation-based feed

Abstract

The invention belongs to the technical field of river snail fermented feed, and particularly relates to river snail feed based on probiotic fermentation and a preparation method of the river snail feed. The feed for the field snails based on probiotic fermentation is prepared from the following raw materials in parts by mass: 20 to 35 parts of soybean meal, 3 to 8 parts of fish meal, 15 to 25 parts of corn flour, 10 to 30 parts of wheat bran, 0.5 to 1.2 parts of a vitamin mixture, 0.5 to 1.2 parts of a mineral substance mixture, 1 to 3 parts of compound probiotic powder, 1.12 to 2.0 parts of shell powder, 2 to 3 parts of soybean oil and 0.1 to 0.3 part of beta-glucan. The prepared feed for the Chinese escargots can be used for feeding the Chinese escargots. And tests show that the produced feed for the Chinese round escargots can significantly improve the individual weight, the acre yield, the survival rate and the meat percentage of the Chinese round escargots, and has high and significant nutrition.

Description

Technical Field

[0001] The invention belongs to the technical field of fermented field snail feed, and particularly relates to a Chinese round field snail feed based on probiotic fermentation and a preparation method thereof. Background Art

[0002] With the rapid development of my country's economy and people's continuous pursuit of quality of life, people's requirements for the quality of aquatic products are getting higher and higher. As snails are an important raw material for Guangxi snail noodles, people's consumption demand for snails and their products is increasing, and the snail farming industry has also developed rapidly.

[0003] However, during the rapid development process, many shortcomings have gradually been exposed. In the existing Chinese round field snail feed, soybean meal and other unprocessed raw materials are basically compounded, which easily leads to problems of single feed ingredients and poor palatability. Summary of the Invention

[0004] To address the issues in existing Chinese round field snail feeds, which primarily utilize unprocessed raw materials such as soybean meal, resulting in a single ingredient and poor palatability, the present invention provides a Chinese round field snail feed fermented with probiotics and a preparation method thereof. To achieve the above objectives, the present invention employs the following technical solutions.

[0005] The invention provides a Chinese round field snail feed based on probiotic fermentation. The feed comprises the following raw materials, measured by weight: 20 to 35 parts of soybean meal, 3 to 8 parts of fish meal, 15 to 25 parts of corn flour, 10 to 30 parts of wheat bran, 0.5 to 1.2 parts of a vitamin mixture, 0.5 to 1.2 parts of a mineral mixture, 1 to 3 parts of a compound probiotic powder, 1.12 to 2.0 parts of shell powder, 2 to 3 parts of soybean oil, and 0.1 to 0.3 parts of beta-glucan.

[0006] The vitamin mixture comprises the following raw materials, measured in parts by mass: 20 to 30 parts of vitamin A, 40 to 64 parts of vitamin E, 0.5 to 3 parts of vitamin D, 15 to 20 parts of vitamin B1, 1.5 to 2 parts of vitamin B2, 18 to 37 parts of vitamin PP, 6 to 11 parts of calcium pantothenate, 50 to 60 parts of vitamin B6, 3 to 6 parts of vitamin B12, 0.2 to 0.25 parts of biotin, 2 to 5 parts of folic acid, and 210 to 310 parts of vitamin C.

[0007] The mineral mixture comprises the following raw materials, calculated by weight: 14 to 20 parts of copper sulfate, 70 to 120 parts of ferrous sulfate, 22 to 65 parts of zinc sulfate, 0.2 to 1.1 parts of sodium selenite, 13 to 32 parts of potassium dihydrogen phosphate, and 11 to 20 parts of sodium dihydrogen phosphate.

[0008] The composite probiotic powder is prepared by mixing freeze-dried bacterial powder of Candida utilis and freeze-dried bacterial powder of Bacillus pumilus in a mass ratio of 1:0.5-1.5.

[0009] According to the nutritional requirements of Chinese round field snails and in combination with actual needs, soybean meal and fish meal are used to supplement protein, corn flour and wheat bran are used to supplement carbohydrates, a vitamin mixture, a mineral mixture and shell powder are used to supplement trace elements, soybean oil is used to supplement fat, and a probiotic mixture is used to provide raw material fermentation conditions and supplement intestinal probiotics. The Chinese round field snail feed formula based on probiotic fermentation is formulated according to the present invention. The formula provided by the present invention can significantly reduce the anti-nutritional factors in the raw materials and increase the flavor factors, solving the problems of the single feed ingredients and poor palatability in existing Chinese round field snail feeds.

[0010] Preferably, the Chinese round field snail feed comprises the following raw materials, measured by mass: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of vitamin mixture, 1 part of mineral mixture, 2 parts of compound probiotic powder, 1.8 parts of shell powder, 3 parts of soybean oil and 0.2 parts of β-glucan.

[0011] Preferably, the vitamin mixture comprises the following raw materials by mass: 25 parts of vitamin A, 54 parts of vitamin E, 1.5 parts of vitamin D, 16 parts of vitamin B1, 1.6 parts of vitamin B2, 19 parts of vitamin PP, 7 parts of calcium pantothenate, 53 parts of vitamin B6, and 10 parts of vitamin B6. 12 3.4 parts, biotin 0.21 parts, folic acid 3.8 parts and vitamin C 237 parts.

[0012] Preferably, the mineral mixture comprises the following raw materials, calculated by weight: 16.3 parts of copper sulfate, 89.4 parts of ferrous sulfate, 42.1 parts of zinc sulfate, 0.58 parts of sodium selenite, 25.8 parts of potassium dihydrogen phosphate and 13.7 parts of sodium dihydrogen phosphate.

[0013] Preferably, the composite probiotic powder is prepared by mixing the freeze-dried bacterial powder of Candida utilis and the freeze-dried bacterial powder of Bacillus pumilus in a mass ratio of 1:1.

[0014] The total bacterial count of the composite probiotic powder is 35 billion / gram.

[0015] The preparation method of the composite probiotic powder comprises the following steps: Candida utilis purchased from the China Agricultural Microbial Culture Collection was activated and cultured to the plateau phase. The culture medium was centrifuged at 1000 g for 10 minutes. The supernatant was discarded, and the Candida utilis precipitate was washed three times with PBS to obtain Precipitate 1.

[0016] Bacillus pumilus purchased from the China Agricultural Microbial Culture Collection was activated and cultured to the plateau phase. The culture medium was centrifuged at 1000 g for 10 minutes. The supernatant was discarded, and the Bacillus pumilus precipitate was washed three times with PBS to obtain Precipitate 2.

[0017] The washed precipitate was placed in a freeze dryer and dried for 48 hours to obtain freeze-dried bacterial powder of Candida utilis.

[0018] The washed precipitate was placed in a freeze dryer and dried for 48 hours to obtain freeze-dried bacterial powder of Bacillus pumilus.

[0019] The composite probiotic powder is obtained by mixing freeze-dried bacterial powder of Candida utilis and freeze-dried bacterial powder of Bacillus pumilus in a mass ratio of 1:1.

[0020] The present invention also provides a method for preparing Chinese round field snail feed based on probiotic fermentation, comprising the following steps: Soybean meal, fish meal, corn flour, wheat bran, vitamin mixture, mineral mixture, compound probiotic powder, shell powder, soybean oil and β-glucan were weighed respectively according to mass.

[0021] The soybean meal, fish meal, corn meal, wheat bran and shell powder are mixed evenly, crushed and sieved to obtain the primary processed material.

[0022] The soybean oil, the beta-glucan and the pre-treated material are mixed to obtain a material to be fermented.

[0023] Water was added to the material to be fermented, and then the composite probiotic powder was added to obtain the primary fermented feed for the first fermentation; the volume of the water added to 1g of the material to be fermented was 4.8×10 5 mL~5.2×10 5 mL.

[0024] The vitamin mixture, the mineral mixture and the primary fermentation feed are mixed evenly, and then fermented for a second time to obtain the final fermentation feed, namely the Chinese round field snail feed.

[0025] By adopting the preparation method provided by the present invention, the raw materials are mixed in corresponding proportions and then fermented with probiotics, which can significantly reduce the anti-nutritional factors in the raw materials and increase the flavor factors, thereby solving the problems of single feed ingredients and poor palatability in existing Chinese round field snail feed.

[0026] Preferably, the sieving refers to sieving through a 40-60 mesh sieve.

[0027] Preferably, the temperature of the first fermentation is 22° C. to 25° C., the humidity of the first fermentation is 60% to 70%, and the time of the first fermentation is 48.

[0028] Preferably, the temperature of the second fermentation is 30° C. to 35° C., the humidity of the second fermentation is 60% to 70%, and the time of the second fermentation is 24 hours.

[0029] Preferably, the Chinese round field snail feed is dried at a constant temperature of 50° C. to 60° C. and then sealed for storage.

[0030] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention provides a Chinese round field snail feed based on probiotic fermentation, which comprises the following raw materials, by weight: 20-35 parts soybean meal, 3-8 parts fish meal, 15-25 parts corn flour, 10-30 parts wheat bran, 0.5-1.2 parts vitamin mixture, 0.5-1.2 parts mineral mixture, 1-3 parts compound probiotic powder, 1.12-2.0 parts shell powder, 2-3 parts soybean oil, and 0.1-0.3 parts beta-glucan. The vitamin mixture comprises the following raw materials: vitamin A, vitamin E, vitamin D, vitamin B1, vitamin B2, vitamin PP, calcium pantothenate, vitamin B6, vitamin B12, biotin, folic acid, and vitamin C; the mineral mixture comprises the following raw materials: copper sulfate, ferrous sulfate, zinc sulfate, sodium selenite, potassium dihydrogen phosphate, and sodium dihydrogen phosphate; and the compound probiotic powder comprises Candida utilis and Bacillus pumilus. By combining the above raw materials with Candida utilis and Bacillus pumilus, the anti-nutritional factors in the raw materials are greatly reduced and the flavor factors are added, thus solving the problems of single feed ingredients and poor palatability in existing feeds.

[0031] 2. The Chinese round snail feed based on probiotic fermentation provided by the present invention enriches the nutritional composition of snail feed and improves the growth performance and quality of snails.

[0032] 3. The Chinese round field snail feed based on probiotic fermentation provided by the present invention adopts composite probiotic powder + segmented fermentation process to process soybean meal, and its main advantages are: the fermentation method of the present invention adopts Candida utilis + Bacillus pumilus + segmented fermentation technology to ferment the feed. First, Candida utilis and Bacillus pumilus are used to perform preliminary fermentation on the raw materials. By utilizing the growth characteristics of the strains, the utilization value of the fermented feed of solid soybean meal, corn flour and wheat bran in the raw materials is improved, the anti-nutritional factors and soybean meal toxicity in soybean meal are reduced, and the palatability, appetitiveness and nutritional value of soybean meal, corn flour and wheat bran are improved. Then, a certain amount of multivitamins and multiminerals are added to the fermentation product in the segmented fermentation process to further improve the nutritional value of the fermented feed. The present invention finally uses the prepared fermented feed for the feeding of Chinese round field snails. After testing, the produced fermented feed can significantly improve the individual weight, per mu yield, survival rate and meat yield of Chinese round field snails, and has high and significant nutritional value. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is the test result of the field snail breeding index in the present invention.

[0034] Figure 2 This is a diagram of the feed for the Chinese round pond snail of the present invention. DETAILED DESCRIPTION

[0035] The present invention will be described in detail below with reference to specific examples and accompanying drawings, but this should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0036] The strains and their sources in the embodiments of the present invention are as follows: Candida utilis: Candida utilis ( Candida utilis ); from the China Agricultural Microbial Culture Collection Center, strain number: ACCC 20060.

[0037] Bacillus pumilus: Bacillus pumilus C-3 ( Bacillus pumilus ); from the China Agricultural Microbiological Culture Collection Center, strain number: ACCC 04447.

[0038] Example 1 The raw materials for preparing a Chinese round field snail feed based on probiotic fermentation are composed of the following components in parts by mass: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 2 parts of a compound probiotic powder, 1.8 parts of shell powder, 3 parts of soybean oil and 0.2 parts of β-glucan.

[0039] The vitamin mixture is composed of the following components by mass: 25 parts of vitamin A, 54 parts of vitamin E, 1.5 parts of vitamin D, 16 parts of vitamin B1, 1.6 parts of vitamin B2, 19 parts of vitamin PP, 7 parts of calcium pantothenate, 53 parts of vitamin B6, and 10 parts of vitamin B1. 12 3.4 parts, biotin 0.21 parts, folic acid 3.8 parts and vitamin C 237 parts.

[0040] The preparation method of the above vitamin mixture is as follows: Weigh each raw material according to the mass ratio of the vitamin mixture, mix them evenly to obtain the vitamin mixture.

[0041] The mineral mixture consists of the following components in parts by mass: 16.3 parts of copper sulfate, 89.4 parts of ferrous sulfate, 42.1 parts of zinc sulfate, 0.58 parts of sodium selenite, 25.8 parts of potassium dihydrogen phosphate and 13.7 parts of sodium dihydrogen phosphate.

[0042] The preparation method of the above-mentioned mineral mixture is as follows: Weigh each raw material according to the weight ratio of the mineral element mixture, mix them evenly to obtain a mineral mixture.

[0043] The composite probiotic powder is prepared by mixing freeze-dried powder of Candida utilis and freeze-dried powder of Bacillus pumilus in a mass ratio of 1:1. The specific preparation method includes the following steps: Candida utilis purchased from the China Agricultural Microbial Culture Collection was activated in YEPD liquid medium and cultured to the plateau phase. The culture medium was centrifuged at 1000g for 10 minutes. The supernatant was discarded, and the Candida utilis precipitate was washed three times with PBS to obtain Precipitate 1.

[0044] Bacillus pumilus purchased from the China Agricultural Microbial Culture Collection was activated in LB liquid medium and cultured to the plateau phase. The culture medium was placed in a centrifuge and centrifuged at 1000 g for 10 minutes. The supernatant was discarded, and the Bacillus pumilus precipitate was washed three times with PBS to obtain Precipitate 2.

[0045] The washed precipitate was placed in a freeze dryer and dried for 48 hours to obtain freeze-dried bacterial powder of Candida utilis.

[0046] The washed precipitate was placed in a freeze dryer and dried for 48 hours to obtain freeze-dried bacterial powder of Bacillus pumilus.

[0047] The freeze-dried bacterial powder of Candida utilis and the freeze-dried bacterial powder of Bacillus pumilus were mixed in a mass ratio of 1:1 to obtain a composite probiotic powder. After dilution testing, the total bacterial count of the compound probiotic powder is greater than 35 billion / gram.

[0048] YEPD medium was purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd.

[0049] LB medium was purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0050] Fish meal was purchased from Guangzhou Xinbijie Muliang Feed Co., Ltd.

[0051] Corn flour was purchased from Binzhou Jindingyi Extruded Feed Co., Ltd.

[0052] Wheat bran was purchased from Shijiazhuang Lvwan Agricultural and Sideline Products Co., Ltd.

[0053] Shell powder was purchased from Qingdao Dongtai Shell Technology Co., Ltd.

[0054] The preparation method of the Chinese round field snail feed based on probiotic fermentation comprises the following steps: S1. Weigh soybean meal, fish meal, corn flour, wheat bran, vitamin mixture, mineral mixture, compound probiotic powder, shell powder, soybean oil and β-glucan according to mass and set aside.

[0055] S2. After uniformly mixing soybean meal, fish meal, corn flour, wheat bran and shell powder, the mixture is pulverized with a grinder and passed through a 60-mesh sieve to obtain a primary processed material.

[0056] S3. Fully mix the soybean oil, β-glucan and the initially treated material to obtain a material to be fermented.

[0057] S4. Add an appropriate amount of water to the material to be fermented, adjust the humidity to a range suitable for probiotic fermentation, then add the composite probiotic powder, stir thoroughly for 20 minutes, and ferment under constant temperature and humidity conditions of 25°C and 65% humidity for 48 hours to obtain the primary fermented feed.

[0058] S5. Fully stir the vitamin mixture, mineral mixture and primary fermentation feed for 30 minutes, and ferment them under constant temperature and humidity conditions of 33°C and 65% humidity for 24 hours to obtain the final fermentation feed, i.e., Chinese round pond snail feed.

[0059] S6. The prepared Chinese round field snail feed is placed in a constant temperature of 60° C. for drying and then sealed for storage before use.

[0060] Example 2 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 20 parts of soybean meal, 3 parts of fish meal, 15 parts of corn flour, 10 parts of wheat bran, 0.5 parts of a vitamin mixture, 0.5 parts of a mineral mixture, 1 part of a compound probiotic powder, 1.12 parts of shell powder, 2 parts of soybean oil, and 0.1 parts of beta-glucan.

[0061] The vitamin mixture is composed of the following components by mass: 25 parts of vitamin A, 54 parts of vitamin E, 1.5 parts of vitamin D, 16 parts of vitamin B1, 1.6 parts of vitamin B2, 19 parts of vitamin PP, 7 parts of calcium pantothenate, 53 parts of vitamin B6, and 10 parts of vitamin B1. 12 3.4 parts, biotin 0.21 parts, folic acid 3.8 parts and vitamin C 237 parts.

[0062] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0063] The mineral mixture consists of the following components in parts by mass: 16.3 parts of copper sulfate, 89.4 parts of ferrous sulfate, 42.1 parts of zinc sulfate, 0.58 parts of sodium selenite, 25.8 parts of potassium dihydrogen phosphate and 13.7 parts of sodium dihydrogen phosphate.

[0064] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0065] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0066] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1.

[0067] Example 3 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed are composed of the following components in parts by weight: 35 parts of soybean meal, 8 parts of fish meal, 25 parts of corn flour, 30 parts of wheat bran, 1.2 parts of a vitamin mixture, 1.2 parts of a mineral mixture, 3 parts of a compound probiotic powder, 2.0 parts of shell powder, 3 parts of soybean oil, and 0.3 parts of beta-glucan.

[0068] The vitamin mixture is composed of the following components by mass: 25 parts of vitamin A, 54 parts of vitamin E, 1.5 parts of vitamin D, 16 parts of vitamin B1, 1.6 parts of vitamin B2, 19 parts of vitamin PP, 7 parts of calcium pantothenate, 53 parts of vitamin B6, and 10 parts of vitamin B1. 12 3.4 parts, biotin 0.21 parts, folic acid 3.8 parts and vitamin C 237 parts.

[0069] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0070] The mineral mixture consists of the following components in parts by mass: 16.3 parts of copper sulfate, 89.4 parts of ferrous sulfate, 42.1 parts of zinc sulfate, 0.58 parts of sodium selenite, 25.8 parts of potassium dihydrogen phosphate and 13.7 parts of sodium dihydrogen phosphate.

[0071] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0072] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0073] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1.

[0074] Example 4 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 2 parts of a compound probiotic powder, 1.8 parts of shell powder, 3 parts of soybean oil, and 0.2 parts of beta-glucan.

[0075] The vitamin mixture is composed of the following components by mass: 20 parts of vitamin A, 40 parts of vitamin E, 0.5 parts of vitamin D, 15 parts of vitamin B1, 1.5 parts of vitamin B2, 18 parts of vitamin PP, 6 parts of calcium pantothenate, 50 parts of vitamin B6, and 100 parts of vitamin B1. 12 3 parts, biotin 0.2 parts, folic acid 2 parts and vitamin C 210 parts.

[0076] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0077] The mineral mixture consists of the following components in parts by mass: 14 parts of copper sulfate, 70 parts of ferrous sulfate, 22 parts of zinc sulfate, 0.2 parts of sodium selenite, 13 parts of potassium dihydrogen phosphate and 11 parts of sodium dihydrogen phosphate.

[0078] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0079] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0080] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1.

[0081] Example 5 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 2 parts of a compound probiotic powder, 1.8 parts of shell powder, 3 parts of soybean oil, and 0.2 parts of beta-glucan.

[0082] The vitamin mixture is composed of the following components by mass: 30 parts of vitamin A, 64 parts of vitamin E, 3 parts of vitamin D, 20 parts of vitamin B1, 2 parts of vitamin B2, 37 parts of vitamin PP, 11 parts of calcium pantothenate, 60 parts of vitamin B6, and 10 parts of vitamin B1. 12 6 parts, biotin 0.25 parts, folic acid 5 parts and vitamin C 310 parts.

[0083] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0084] The mineral mixture consists of the following components in parts by mass: 20 parts of copper sulfate, 120 parts of ferrous sulfate, 65 parts of zinc sulfate, 1.1 parts of sodium selenite, 32 parts of potassium dihydrogen phosphate and 20 parts of sodium dihydrogen phosphate.

[0085] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0086] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0087] Example 6 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 20 parts of soybean meal, 3 parts of fish meal, 15 parts of corn flour, 10 parts of wheat bran, 0.5 parts of a vitamin mixture, 0.5 parts of a mineral mixture, 1 part of a compound probiotic powder, 1.12 parts of shell powder, 2 parts of soybean oil, and 0.1 parts of beta-glucan.

[0088] The vitamin mixture is composed of the following components by mass: 20 parts of vitamin A, 40 parts of vitamin E, 0.5 parts of vitamin D, 15 parts of vitamin B1, 1.5 parts of vitamin B2, 18 parts of vitamin PP, 6 parts of calcium pantothenate, 50 parts of vitamin B6, and 100 parts of vitamin B1. 12 3 parts, biotin 0.2 parts, folic acid 2 parts and vitamin C 210 parts.

[0089] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0090] The mineral mixture consists of the following components in parts by mass: 14 parts of copper sulfate, 70 parts of ferrous sulfate, 22 parts of zinc sulfate, 0.2 parts of sodium selenite, 13 parts of potassium dihydrogen phosphate and 11 parts of sodium dihydrogen phosphate.

[0091] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0092] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0093] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1.

[0094] Example 7 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 20 parts of soybean meal, 3 parts of fish meal, 15 parts of corn flour, 10 parts of wheat bran, 0.5 parts of a vitamin mixture, 0.5 parts of a mineral mixture, 1 part of a compound probiotic powder, 1.12 parts of shell powder, 2 parts of soybean oil, and 0.1 parts of beta-glucan.

[0095] The vitamin mixture is composed of the following components by mass: 30 parts of vitamin A, 64 parts of vitamin E, 3 parts of vitamin D, 20 parts of vitamin B1, 2 parts of vitamin B2, 37 parts of vitamin PP, 11 parts of calcium pantothenate, 60 parts of vitamin B6, and 10 parts of vitamin B1. 12 6 parts, biotin 0.25 parts, folic acid 5 parts and vitamin C 310 parts.

[0096] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0097] The mineral mixture consists of the following components in parts by mass: 20 parts of copper sulfate, 120 parts of ferrous sulfate, 65 parts of zinc sulfate, 1.1 parts of sodium selenite, 32 parts of potassium dihydrogen phosphate and 20 parts of sodium dihydrogen phosphate.

[0098] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0099] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0100] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1.

[0101] Example 8 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed are composed of the following components in parts by weight: 35 parts of soybean meal, 8 parts of fish meal, 25 parts of corn flour, 30 parts of wheat bran, 1.2 parts of a vitamin mixture, 1.2 parts of a mineral mixture, 3 parts of a compound probiotic powder, 2.0 parts of shell powder, 3 parts of soybean oil, and 0.3 parts of beta-glucan.

[0102] The vitamin mixture is composed of the following components by mass: 20 parts of vitamin A, 40 parts of vitamin E, 0.5 parts of vitamin D, 15 parts of vitamin B1, 1.5 parts of vitamin B2, 18 parts of vitamin PP, 6 parts of calcium pantothenate, 50 parts of vitamin B6, and 100 parts of vitamin B1. 12 3 parts, biotin 0.2 parts, folic acid 2 parts and vitamin C 210 parts.

[0103] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0104] The mineral mixture consists of the following components in parts by mass: 14 parts of copper sulfate, 70 parts of ferrous sulfate, 22 parts of zinc sulfate, 0.2 parts of sodium selenite, 13 parts of potassium dihydrogen phosphate and 11 parts of sodium dihydrogen phosphate.

[0105] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0106] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0107] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1.

[0108] Example 9 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 2 parts of a compound probiotic powder, 1.8 parts of shell powder, 3 parts of soybean oil, and 0.2 parts of beta-glucan.

[0109] The vitamin mixture is composed of the following components by mass: 30 parts of vitamin A, 64 parts of vitamin E, 3 parts of vitamin D, 20 parts of vitamin B1, 2 parts of vitamin B2, 37 parts of vitamin PP, 11 parts of calcium pantothenate, 60 parts of vitamin B6, and 10 parts of vitamin B1. 12 6 parts, biotin 0.25 parts, folic acid 5 parts and vitamin C 310 parts.

[0110] The preparation method of the above vitamin mixture is the same as that of Example 1.

[0111] The mineral mixture consists of the following components in parts by mass: 20 parts of copper sulfate, 120 parts of ferrous sulfate, 65 parts of zinc sulfate, 1.1 parts of sodium selenite, 32 parts of potassium dihydrogen phosphate and 20 parts of sodium dihydrogen phosphate.

[0112] The preparation method of the above-mentioned mineral mixture is the same as that of Example 1.

[0113] The preparation method and sources of the composite probiotic powder, fish meal, corn flour, wheat bran and shell powder are the same as those in Example 1.

[0114] Comparative Example 1 A Chinese round field snail feed based on probiotic fermentation is prepared by using raw materials consisting of the following components in parts by weight: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 2 parts of Candida utilis powder, 1.8 parts of shell powder, 3 parts of soybean oil, and 0.2 parts of beta-glucan.

[0115] The vitamin mixture is composed of the following components in parts by mass and its preparation method is the same as that in Example 1.

[0116] The mineral mixture consists of the following components in parts by mass and its preparation method is the same as that in Example 1.

[0117] The preparation method of Candida utilis powder is the same as the preparation method of freeze-dried Candida utilis powder in Example 1.

[0118] The preparation method and sources of fish meal, corn meal, wheat bran and shell meal are the same as those in Example 1.

[0119] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1, except that the formula in Example 1 is replaced with the formula of Comparative Example 1.

[0120] Comparative Example 2 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 2 parts of Bacillus pumilus powder, 1.8 parts of shell powder, 3 parts of soybean oil, and 0.2 parts of beta-glucan.

[0121] The vitamin mixture is composed of the following components in parts by mass and its preparation method is the same as that in Example 1.

[0122] The mineral mixture consists of the following components in parts by mass and its preparation method is the same as that in Example 1.

[0123] The method for preparing Bacillus pumilus powder is the same as the method for preparing freeze-dried Bacillus pumilus powder in Example 1.

[0124] The preparation method and sources of fish meal, corn meal, wheat bran and shell meal are the same as those in Example 1.

[0125] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1, except that the formula in Example 1 is replaced with the formula of Comparative Example 2.

[0126] Example 3 A Chinese round field snail feed based on probiotic fermentation, wherein the raw materials for preparing the feed consist of the following components in parts by weight: 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of a vitamin mixture, 1 part of a mineral mixture, 1.8 parts of shell powder, 3 parts of soybean oil and 0.2 parts of beta-glucan.

[0127] The vitamin mixture is composed of the following components in parts by mass and its preparation method is the same as that in Example 1.

[0128] The mineral mixture consists of the following components in parts by mass and its preparation method is the same as that in Example 1.

[0129] The preparation method and sources of fish meal, corn meal, wheat bran and shell meal are the same as those in Example 1.

[0130] The preparation method of the above-mentioned Chinese round field snail feed based on probiotic fermentation is the same as that in Example 1, except that the formula in Example 1 is replaced with the formula of Comparative Example 3.

[0131] In order to illustrate the effects of the probiotic fermentation-based Chinese round field snail feed and the preparation method thereof provided by the present invention, the Chinese round field snail feeds prepared in Example 1 and Comparative Examples 1 to 3 were selected and fed to the field snails, respectively, and the breeding experiments shown below were carried out.

[0132] Four groups, each with three replicates, were designed for Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3. The experiments were conducted in paddy fields with flowing spring water, with each group rearing the snails in a different paddy field. Chinese round snails were collected from central and eastern China and safely introduced to southern China for rearing. The introduced snails were domesticated in paddy fields with flowing spring water. After acclimation, each group was fed a corresponding diet at a rate of 2.5% of the snail's body weight.

[0133] The snails of Example 1 and Comparative Examples 1 to 3 were tested for individual weight, yield per mu, survival rate and meat yield.

[0134] The specific detection methods are as follows: Individual weight: 20 cultured Chinese round pond snails in each group were randomly selected and weighed and recorded.

[0135] Yield per mu: Count and record the yield of farmed Chinese round pond snails per mu.

[0136] Survival rate: Count the number of seedlings before launching the breeding test, and calculate the number when calculating the per-acre yield.

[0137] Meat yield: After shelling the weighed Chinese round snails, weigh and record the snail meat.

[0138] The test results are shown in Table 1.

[0139] Table 1 Test results of snail breeding indicators As shown in Table 1, the Chinese round field snail feed fermented with probiotics in Example 1 is beneficial to the single weight, per mu yield, survival rate and meat yield of the field snail during the breeding process. The single weight of the field snail in Example 1 is increased by 18.75%, the per mu yield of the field snail is increased by 9.08%, and the survival rate and meat yield of the field snail are increased by 8.63% and 30.44%, respectively. The single weight of the field snail in Example 1 is increased by 25%, the per mu yield of the field snail is increased by 12.91%, and the survival rate and meat yield of the field snail are increased by 6.44% and 31.71%, respectively. The single weight of the field snail in Example 1 is increased by 43.75%, the per mu yield of the field snail is increased by 23.17%, and the survival rate and meat yield of the field snail are increased by 14.87% and 45.38%, respectively, compared with the comparative example 3. The single weight, per mu yield, survival rate and meat yield of the field snail are significantly improved, and the fermented feed has high and significant nutritional value.

[0140] It can be seen that the Chinese round field snail feed based on probiotic fermentation prepared by the present invention has a significant improvement on the individual weight, yield per mu, survival rate and meat yield of the Chinese round field snail, indicating that the Chinese round field snail feed based on probiotic fermentation prepared by the present invention can effectively solve the problems of single feed ingredients and poor palatability in the existing Chinese round field snail feed.

[0141] It should be noted that when numerical ranges are mentioned in the present invention, it should be understood that both endpoints of each numerical range and any value between the two endpoints may be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes preferred embodiments. Although preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they understand the basic inventive concepts. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0142] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A Chinese round field snail feed based on probiotic fermentation, characterized in that: The preparation comprises the following raw materials, calculated by weight: 20 to 35 parts of soybean meal, 3 to 8 parts of fish meal, 15 to 25 parts of corn flour, 10 to 30 parts of wheat bran, 0.5 to 1.2 parts of vitamin mixture, 0.5 to 1.2 parts of mineral mixture, 1 to 3 parts of compound probiotic powder, 1.12 to 2.0 parts of shell powder, 2 to 3 parts of soybean oil and 0.1 to 0.3 parts of beta-glucan; The vitamin mixture comprises the following raw materials in parts by mass: 20 to 30 parts of vitamin A, 40 to 64 parts of vitamin E, 0.5 to 3 parts of vitamin D, 15 to 20 parts of vitamin B1, 1.5 to 2 parts of vitamin B2, 18 to 37 parts of vitamin PP, 6 to 11 parts of calcium pantothenate, 50 to 60 parts of vitamin B6, and 10 to 20 parts of vitamin B1. 12 3 to 6 parts, biotin 0.2 to 0.25 parts, folic acid 2 to 5 parts and vitamin C 210 to 310 parts; The mineral mixture comprises the following raw materials, calculated by weight: 14 to 20 parts of copper sulfate, 70 to 120 parts of ferrous sulfate, 22 to 65 parts of zinc sulfate, 0.2 to 1.1 parts of sodium selenite, 13 to 32 parts of potassium dihydrogen phosphate, and 11 to 20 parts of sodium dihydrogen phosphate; The composite probiotic powder is prepared by mixing freeze-dried bacterial powder of Candida utilis and freeze-dried bacterial powder of Bacillus pumilus in a mass ratio of 1:0.5-1.

5.

2. A Chinese round field snail feed based on probiotic fermentation according to claim 1, characterized in that, The preparation raw materials include, by mass, 35 parts of soybean meal, 5 parts of fish meal, 22 parts of corn flour, 30 parts of wheat bran, 1 part of vitamin mixture, 1 part of mineral mixture, 2 parts of compound probiotic powder, 1.8 parts of shell powder, 3 parts of soybean oil and 0.2 parts of beta-glucan.

3. A Chinese round field snail feed based on probiotic fermentation according to claim 2, characterized in that, The vitamin mixture comprises the following raw materials by weight: 25 parts of vitamin A, 54 parts of vitamin E, 1.5 parts of vitamin D, 16 parts of vitamin B1, 1.6 parts of vitamin B2, 19 parts of vitamin PP, 7 parts of calcium pantothenate, 53 parts of vitamin B6, and 10 parts of vitamin B6. 12 3.4 parts, biotin 0.21 parts, folic acid 3.8 parts and vitamin C 237 parts.

4. A Chinese round field snail feed based on probiotic fermentation according to claim 2, characterized in that, The mineral mixture includes the following raw materials, calculated by weight: 16.3 parts of copper sulfate, 89.4 parts of ferrous sulfate, 42.1 parts of zinc sulfate, 0.58 parts of sodium selenite, 25.8 parts of potassium dihydrogen phosphate, and 13.7 parts of sodium dihydrogen phosphate.

5. The Chinese round field snail feed based on probiotic fermentation according to claim 1, characterized in that: The composite probiotic powder is prepared by mixing the freeze-dried bacterial powder of Candida utilis and the freeze-dried bacterial powder of Bacillus pumilus in a mass ratio of 1:

1. The total bacterial count of the composite probiotic powder is 35 billion / gram.

6. The method for preparing a Chinese round field snail feed based on probiotic fermentation according to any one of claims 1 to 5, characterized in that: The steps include: Weigh soybean meal, fish meal, corn flour, wheat bran, vitamin mixture, mineral mixture, compound probiotic powder, shell powder, soybean oil and β-glucan respectively by mass; Soybean meal, fish meal, corn meal, wheat bran and shell powder are mixed evenly, crushed and sieved to obtain the primary processed material; mixing the soybean oil, the β-glucan and the pre-treated material to obtain a material to be fermented; Water was added to the material to be fermented, and then the composite probiotic powder was added to obtain the primary fermented feed for the first fermentation; the volume of the water added to 1g of the material to be fermented was 4.8×10 5 mL~5.2×10 5 mL; The vitamin mixture, the mineral mixture and the primary fermentation feed are mixed evenly, and then fermented for a second time to obtain the final fermentation feed, namely the Chinese round field snail feed.

7. The preparation method according to claim 6, characterized in that The sieving refers to sieving through a 40-60 mesh sieve.

8. The preparation method according to claim 6, characterized in that The temperature of the first fermentation is 22° C. to 25° C., the humidity of the first fermentation is 60% to 70%, and the time of the first fermentation is 48.

9. The preparation method according to claim 6, characterized in that The temperature of the second fermentation is 30° C. to 35° C., the humidity of the second fermentation is 60% to 70%, and the time of the second fermentation is 24 hours.

10. The preparation method according to claim 6, characterized in that The Chinese round pond snail feed is dried at a constant temperature of 50° C. to 60° C. and then sealed for storage.

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