Yellow-removing and brightening composition and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a yellowing-removing and brightening composition and application thereof. The yellowing-removing and brightening composition is prepared from the following components: carnosine, an albizia bark extract and trehalose, wherein the weight ratio of the carnosine to the albizia bark extract to the trehalose is (0.05 to 1) to (0.08 to 1) to (0.1 to 2). The yellowing-removing and brightening composition comprises carnosine, an albizia bark extract and trehalose, and can effectively improve the yellowing-removing effect; by limiting the weight ratio of carnosine to albizia bark extract to trehalose to be (0.05-1): (0.08-1): (0.1-2), the brightening effect can be improved; the composition provided by the invention can solve the problems of easy inactivation and low bioavailability of carnosine; and when being applied to preparation of cosmetics, the composition can achieve excellent physical and chemical stability.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a yellowing-removing and brightening composition and application thereof. Background Art

[0002] With the accelerated pace of modern life, increasing environmental pollution and work pressure, the homeostasis of human skin is seriously affected and the resistance is gradually declining. At the same time, external stimuli and bad living habits also aggravate the problem of dull and yellow skin. Traditional concepts believe that skin yellowing is mainly caused by glycation damage and oxidative damage, but it is difficult to effectively improve skin yellowing by only using antioxidant and anti-glycemic measures. In fact, the skin also produces reactive carbonyl substances (RCS) during metabolism and oxidation. These substances undergo irreversible carbonyl modification with proteins to form carbonylated proteins, making the skin appear more yellow. Therefore, there is an urgent need to develop a synergistic technology system that can integrate antioxidant, anti-glycation and anti-carbonylation to comprehensively block the yellowing reaction network.

[0003] Chinese invention patent CN118975941A designs a moisturizing cosmetic composition, including instant moisturizing ingredients, ingredients that promote hyaluronic acid content and water channel protein expression, ingredients that promote cell autophagy, anti-glycation ingredients and ingredients that repair circadian rhythms, combining endogenous ingredient expression with exogenous supplementation, to achieve long-lasting moisturizing effects by ensuring the water retention and water transport capabilities of the dermis, and directly replenish the stratum corneum structure to prevent water loss. However, the application focuses on moisturizing and does not explore effects such as anti-yellowing and brightening. Summary of the Invention

[0004] The first aspect of the present invention provides a yellowing-removing and brightening composition, comprising the following components: carnosine, Silktree Albizia Bark extract and trehalose.

[0005] The present application study found that the yellowing-removing and brightening composition includes carnosine, Albizia Julibrissin bark extract and trehalose, which can effectively improve the yellowing-removing effect. The three form a synergistic enhancement system targeting the "source-intermediate product-yellowing product", thereby achieving a comprehensive and efficient skin brightening effect; carnosine, on the one hand, resists oxidative damage by scavenging free radicals and protecting mitochondria, and on the other hand, effectively blocks the formation of advanced glycation end products (AGEs) by competitively inhibiting the reaction between proteins and reducing sugars; Albizia Julibrissin bark extract has strong antioxidant and anti-glycation effects, and by targeted inhibition of the key enzyme in the glycation reaction, glyoxalase I, it reduces the accumulation of active carbonyl compounds (such as methylglyoxal) and blocks the generation pathway of advanced glycation end products (AGEs); trehalose can enhance autophagy and remove damaged proteins; the combination of the three improves the system to inhibit the source, block the process and remove the accumulated yellowing factors, achieve anti-yellowing with a multi-pathway synergistic mechanism, and significantly improve the problem of dull skin tone.

[0006] Further research found that by limiting the weight ratio of carnosine, Albizia Julibrissin bark extract and trehalose to (0.05-1): (0.08-1): (0.1-2), the brightening effect can be improved. It may be that the specific ratio of the three significantly reduces the overall carbonyl stress and oxidative stress levels, creating a microenvironment that is less conducive to the occurrence of oxidation / glycation / carbonylation reactions and more conducive to the operation of the cell's own defense and repair mechanisms. However, carnosinase, which is widely present in skin tissue, will quickly hydrolyze exogenous carnosine, resulting in a significant reduction in its bioavailability. However, this study found that the synergistic effect of Albizia Julibrissin bark extract and trehalose can effectively inhibit carnosinase, improve the stability and bioavailability of carnosine, and effectively improve the anti-yellowing efficiency.

[0007] Optionally, the trehalose comprises crystalline trehalose.

[0008] The weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is (0.05-1): (0.08-1): (0.1-2).

[0009] Optionally, the weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is 1:(0.5-3):(1-5).

[0010] Optionally, the weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is 1:(1-2):(2-4).

[0011] Optionally, the weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is 3:(3-5):(6-10).

[0012] A second aspect of the present invention provides an application of a yellowing-removing and brightening composition for use in the preparation of cosmetics.

[0013] Optionally, the category of the cosmetics includes at least one of gel, lotion, cream, and liquid.

[0014] The yellowing-removing and brightening composition is added in an amount of 0.05-10 wt % in cosmetics.

[0015] Optionally, the yellowing-removing and brightening composition is added to the cosmetic in an amount of 0.1-6 wt %.

[0016] Optionally, the yellowing-removing and brightening composition is added to the cosmetic in an amount of 0.23-4 wt %.

[0017] Beneficial effect 1

[0018] 1. The yellowing-removing and brightening composition includes carnosine, Albizia Julibrissin bark extract and trehalose, which can effectively improve the yellowing-removing effect.

[0019] 2. By limiting the weight ratio of carnosine, Albizia Julibrissin bark extract and trehalose to (0.05-1): (0.08-1):

[0020] (0.1-2), can achieve the effect of enhancing brightening.

[0021] 3. The composition of the present application can solve the problem of easy inactivation and low bioavailability of carnosine.

[0022] 4. The composition of the present application is used in the preparation of cosmetics and can achieve excellent physical and chemical stability.

[0023] 5. The composition of the present application can be used in various types of cosmetics, including gels, emulsions, creams, and liquids. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The anti-glycation results of the test solution (each group was tested 3 times, *p<0.05, ***p<0.001, compared with Example 2).

[0025] Figure 2 The results of the carnosinase inhibition experiment are shown in Table 1 (each group was tested 3 times, ***p<0.001, compared with Comparative Example 7). Figure 3 This is the b-value improvement result of the 7-day human anti-carbonylation experiment (each group was tested 3 times, *p<0.05, **p<0.01, compared with Application Example 2).

[0026] Figure 4 The results of the human anti-carbonylation experiment before and after establishing the yellowing model (each group was tested 3 times, *p<0.05, **p<0.01, compared with Application Example 2).

[0027] Figure 5 These are experimental photos taken the day after the human anti-carbonylation experiment model was created. DETAILED DESCRIPTION

[0028] The purchasing information of the components in the following examples and comparative examples is as follows:

[0029] Carnosine, manufacturer: Redlin;

[0030] Albizia Julibrissin bark extract, brand: Prodizia, manufacturer: SEDERMA, SAS;

[0031] Trehalose (crystalline trehalose), brand: ViviSugar THS, manufacturer: Tongliao Meihua Biotechnology Co., Ltd.

[0032] Example 1

[0033] A yellowing-removing and brightening composition comprises carnosine, albizzia julibrissin bark extract and trehalose; the weight ratio of the carnosine, the albizzia julibrissin bark extract and the trehalose is 5:8:10.

[0034] Example 2

[0035] A yellowing-removing and brightening composition comprises carnosine, a julibrissin bark extract and trehalose; the weight ratio of the carnosine, the julibrissin bark extract and the trehalose is 3:5:10.

[0036] Example 3

[0037] A yellowing-removing and brightening composition comprises carnosine, a julibrissin bark extract and trehalose; the weight ratio of the carnosine, the julibrissin bark extract and the trehalose is 1:1:2.

[0038] Comparative Example 1

[0039] A composition consisting of carnosine and albizzia julibrissin bark extract; the weight ratio of the carnosine to the albizzia julibrissin bark extract is 0.68:1.13, and the total concentration of the active ingredient (composition) is consistent with that in Example 2.

[0040] Comparative Example 2

[0041] A composition consisting of an Albizia Julibrissin bark extract and trehalose; the weight ratio of the Albizia Julibrissin bark extract to trehalose is 1:2, and the total concentration of the active ingredient (composition) is consistent with that in Example 2.

[0042] Comparative Example 3

[0043] A composition consisting of carnosine and trehalose; the weight ratio of the carnosine to trehalose is 0.42:1.39, and the total concentration of the active ingredients (composition) is consistent with that in Example 2.

[0044] Comparative Example 4

[0045] Containing only the Albizia Julibrissin bark extract, the total concentration of active ingredients (composition) is consistent with that of Comparative Example 7.

[0046] Comparative Example 5

[0047] Contains only trehalose, and the total concentration of the active ingredient (composition) is consistent with that of Comparative Example 7.

[0048] Comparative Example 6

[0049] Contains only carnosine, and the total concentration of active ingredients (composition) is consistent with that in Example 2.

[0050] Comparative Example 7

[0051] A composition consisting of an Albizia Julibrissin bark extract and trehalose, with concentrations corresponding to those of the Albizia Julibrissin bark extract and trehalose in Example 2.

[0052] Performance testing methods and data

[0053] The compositions in the examples and comparative examples were mixed with PBS (phosphate buffered saline) to prepare test solutions for antioxidant testing, anti-glycation testing, stability testing, and carnosinase inhibition testing. The test solution formulas, calculated by weight percentage, are shown in Table 1, where " / " indicates no addition.

[0054] Table 1

[0055]

[0056] 1. Anti-glycation experiment (AGEs inhibition rate)

[0057] The test solutions of Examples 1-3 and Comparative Examples 1-4 were selected for antioxidant experiments.

[0058] (1) Bovine serum albumin solution (80 mg / mL): Weigh 16.0 g of bovine serum albumin and dissolve it in 200 mL of phosphate buffer.

[0059] Glucose solution (240 mg / mL): Weigh 48.0 g of glucose and dissolve it in 200 mL of phosphate buffer.

[0060] The final concentration of bovine serum albumin in each reaction system was 40 mg / mL, and the final concentration of glucose was 120 mg / mL.

[0061] (2) Experimental operation

[0062] The reaction system was prepared according to the amount of each reagent added in Table 2.

[0063] Table 2

[0064] Unit (mL) A B C D 2× glycosylation reaction solution 1 0 1 0 2×test samples 1 1 0 0 PBS 0 1 1 2

[0065] After mixing evenly, incubate at 55°C for 4 days. PBS was used instead of the sample as a negative control, and PBS was used instead of the glycosylation reaction solution as a control system. After the reaction, the incubation solution was cooled to room temperature and centrifuged at 2000 rpm for 5 minutes. The supernatant was filtered through a 0.22 μm filter membrane. 200 μL of the reaction solution was then added to a 96-well plate. The AGES inhibition rate was calculated using a fluorescence microplate reader at an excitation wavelength of 320 nm and an emission wavelength of 460 nm.

[0066]

[0067] The results are as follows Figure 1 As shown, the examples have a significant AGEs inhibitory effect compared to comparative examples 1-4, and the inhibitory effect is significantly reduced when any one component is missing, which proves that carnosine, Albizia Julibrissin bark extract and trehalose have a synergistic effect in inhibiting the formation of AGEs.

[0068] 2. Carnosinase inhibition test

[0069] The test solutions of Comparative Example 7 and Comparative Example 4-5 were selected to carry out the 3-carnosinase inhibition experiment.

[0070] (1) Cell culture:

[0071] Human fibroblasts (HSF) were revived and cultured in DMEM containing 10% fetal bovine serum and 1% double-antibody at 37°C and 5% CO2 until the logarithmic growth phase. When the cell confluence reached 80%-90%, they were digested with 0.25% trypsin-0.02% EDTA. The cells became round and detached from the flask wall. Serum culture medium was added to terminate the process. The suspension was made by pipetting and counted, and the density was adjusted to 5×104 cells / mL.

[0072] (2) Experimental treatment:

[0073] Add 100 μL of cell suspension to each well of a 96-well plate, incubate in an incubator for 24 h to allow cells to adhere to the wall, aspirate and discard the original culture medium, add 100 μL of new culture medium to the experimental control group, and add 100 μL of the test sample to the other groups, shake to mix, and continue incubation for 48 h.

[0074] (3) Enzyme reaction and detection:

[0075] After the incubation, the cells were washed three times with PBS. 50 μL of substrate-containing reaction buffer was added to each well and the cells were incubated at 37°C on a low-speed shaker for 30 min. 50 μL of stop solution was added and the reaction solution was transferred to a centrifuge tube. The cells were centrifuged at 3000 rpm for 10 min. The supernatant was collected and the absorbance OD value of each sample in the supernatant was measured using a spectrophotometer at a wavelength of 405 nm. The blank was used for calibration before the test. The carnosinase inhibition rate was calculated as follows:

[0076]

[0077] The test results are as follows Figure 2 As shown, Comparative Example 7 has a stronger inhibitory effect on carnosinase than Comparative Examples 4-5, with a significant difference. Although the single Albizia Julibrissin bark extract and trehalose have a certain inhibitory effect on carnosinase, after the two are combined, the inhibitory effect is greatly improved at the same concentration. This proves that the combination of the two can effectively inhibit the activity of carnosinase, thereby improving the bioavailability of carnosine in the composition, while also protecting endogenous carnosine and indirectly improving antioxidant capacity.

[0078] 3. Human anti-carbonylation experiment

[0079] The compositions of Examples 1-3, Comparative Examples 1-3, and Comparative Example 6 were used to prepare gels for human anti-carbonylation experiments. The gel formulas are shown in Table 3, where " / " means no addition.

[0080] Carbomer, brand: Carbopol 980 Polymer, supplier: Lubrizol Advanced Materials, Inc.;

[0081] Glycerin, brand: Superol K, supplier: Procter & Gamble International Operations SASingapore Branch;

[0082] Propylene glycol, brand: Zemea Propanediol, supplier: Primient Covation LLC;

[0083] Arginine, brand: L-ARGININE C-GRADE, supplier: Ajinomoto Co., Ltd., Japan;

[0084] The specific steps of preparing the gel include:

[0085] (1) Weigh the materials in phase A, mix them evenly, and then homogenize to disperse all the ingredients completely to obtain a blank matrix;

[0086] (2) Weigh the phase B material, add it to the blank matrix, stir evenly, and homogenize for 5 minutes;

[0087] (3) The pH value of the gel was adjusted to 6 with arginine to obtain the final gel test sample.

[0088] Improvement effect: Test results are as follows: Figure 3As shown, compared with the comparative example 6, the application example 2 has the effect of significantly reducing the yellowness, and compared with the comparative examples 1-3, the application example 2 also has a significant improvement effect, which proves that the combination of carnosine, Albizia Julibrissin bark extract and trehalose can synergistically exert a brightening effect.

[0089] Protective effect: A yellowing model is established by using dihydroxyacetone to react with the skin to form a carbonylation reaction. The protective effect of the composition on carbonylation is as follows: Figure 4 As shown, the application example 2 of the present invention has a significant effect of resisting yellowing and dulling caused by carbonylation compared with the application example 6 and the application examples 1-3, and the combination of the three can also synergistically exert the effect of resisting yellowing and dulling. Figure 5 As shown in the results (wherein the blank group refers to the group without any sample treatment, and the base group refers to the group without any active ingredient added to the sample), the color difference between the area where Example 2 is applied and the normal surrounding skin is small, while the color difference between the area where Comparative Example 6 is applied is still large, and there is basically no color difference when Example 3 is applied, which once again proves that the composition has excellent anti-yellowing and dark yellow skin improvement effects.

[0090] Table 3

[0091]

[0092] Test method: 10 young people (aged 18-40, all skin types) were selected. After washing their arms, a suitable sample area (2cm×2cm) was selected. The basic b-value of each area was collected using a Colorimeter CL400. The technician used a pipette to precisely measure the sample and apply it at a rate of 2mg / cm. 2 , applied twice a day. After 7 days of use, the improvement in the b-value of each area was measured. A 5% dihydroxyacetone emulsion was precisely measured using a pipette to create a carbonylation model (2cm×2cm), with a smear volume of 12μL. The day after modeling, technicians measured the b-value of each area to analyze the patented composition's ability to combat skin dullness caused by carbonylation.

[0093] 4. Stability test

[0094] Stability experiments were conducted on application examples 1-3, application comparison examples 1-3, and application comparison example 6.

[0095] Centrifugation experiment - The test liquid was subjected to high-speed centrifugation (3000r / min, centrifugation for 20min) in a centrifuge to observe the stratification and sedimentation of the sample.

[0096] High and low temperature experiment - test method: the test liquid is stored at 10℃, 45℃ and room temperature (25℃) for 2 weeks, and then the changes in the physical and chemical properties of the samples are observed.

[0097] (1) Color stability: Test the color change of the sample during storage to ensure that the color is uniform and not easy to fade.

[0098] (2) Texture stability: Evaluate the fluidity, viscosity and other texture characteristics of the test sample to ensure that the texture remains unchanged during storage and use without stratification, agglomeration, etc.

[0099] (3) Appearance stability: Observe the changes in the appearance of the test sample during storage, such as whether there are bubbles, precipitation or turbidity.

[0100] (4) pH stability: The pH value of the sample was tested after storage for a certain period of time.

[0101] As shown in Table 4, there is no significant difference in stability among Application Examples 1-3, Comparative Examples 1-3 and Comparative Example 6 after high-speed centrifugation, indicating that the composition of the present invention has good stability.

[0102] As shown in Table 5, after high and low temperature storage, the stability of Application Examples 1-3 is higher than that of Application Comparative Example 6 and Application Comparative Examples 1-3. The pH value of Application Comparative Example 1 increases slightly, and the change range is within 1.0. However, the pH value of Application Comparative Example 6 increases significantly, and the change range reaches more than 1.0, indicating that the composition of the present invention has good stability.

[0103] Table 4

[0104]

[0105]

[0106] Table 5

[0107]

Claims

1. A yellowing and brightening composition, characterized in that: Includes the following ingredients: carnosine, Albizia Julibrissin bark extract and trehalose.

2. The yellowing-removing and brightening composition according to claim 1, characterized in that The weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is (0.05-1): (0.08-1): (0.1-2).

3. The yellowing-removing and brightening composition according to claim 2, characterized in that The weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is 1:(0.5-3):(1-5).

4. The yellowing-removing and brightening composition according to claim 3, characterized in that The weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is 1:(1-2):(2-4).

5. The yellowing-removing and brightening composition according to claim 4, characterized in that The weight ratio of the carnosine, the Albizia Julibrissin bark extract and the trehalose is 3:(3-5):(6-10).

6. An application of the yellowing-removing and brightening composition according to any one of claims 1 to 5, characterized in that: Used in the preparation of cosmetics.

7. The use of the yellowing-removing and brightening composition according to claim 6, characterized in that: The categories of the cosmetics include at least one of gel, lotion, cream, and liquid.

8. The use of the yellowing-removing and brightening composition according to claim 6, characterized in that: The yellowing-removing and brightening composition is added in an amount of 0.05-10 wt % in cosmetics.

9. The use of the yellowing-removing and brightening composition according to claim 8, characterized in that: The yellowing-removing and brightening composition is added in an amount of 0.1-6 wt % in cosmetics.

10. The use of the yellowing-removing and brightening composition according to claim 9, characterized in that: The yellowing-removing and brightening composition is added in an amount of 0.23-4 wt % in cosmetics.

Images