Pickering emulsion composition for protecting vesicle structure as well as preparation method and application of Pickering emulsion composition

Abstract

The invention discloses a Pickering emulsion composition for protecting a vesicle structure as well as a preparation method and application of the Pickering emulsion composition, belongs to the technical field of plant preparations, and solves the problem of poor stability of plant vesicles in application in the prior art. The Pickering composition comprises the following components: 6%-10% of a humectant, 3%-10% of an emollient, 0.1%-0.3% of xanthan gum, 2%-4% of microcrystalline cellulose, 8%-13% of an anti-inflammatory active component and the balance of water, wherein based on the total mass of the Pickering composition, the anti-inflammatory active component comprises 3%-5% of a rose exosome, 2%-4% of a grape exosome and 1%-4% of a purslane exosome. According to the invention, an emulsion system is stabilized by solid particles through the Pickering emulsion, a plant vesicle structure is protected, and dual regulation and control on inflammation and aging are realized.

Description

Technical Field

[0001] The present invention relates to the technical field of plant preparations, and more particularly to a Pickering emulsion composition that protects vesicle structure, and a preparation method and application thereof. Background Art

[0002] In the existing technology, the treatment of inflammatory aging mostly focuses on a single target (such as inhibiting specific inflammatory factors), and there is a lack of solutions to regulate the synergistic effect of chronic inflammation and cellular aging from the source.

[0003] Plant-derived vesicles (PDVs) are bioactive nanoparticles discovered in recent years with potential anti-inflammatory, antioxidant, and anti-aging benefits. However, PDVs are unstable and susceptible to environmental influences during extraction and storage. They are easily degradable, leading to structural damage and loss of function, limiting their application. Furthermore, in the prior art, the emulsifying components of cosmetics are all emulsifiers, but existing emulsifiers can be irritating to the skin.

[0004] Therefore, it is of great significance to develop a composition that can protect the structure of plant vesicles and enhance their anti-inflammatory and anti-aging functions. Summary of the Invention

[0005] The present invention provides a Pickering emulsion composition for protecting vesicle structure, a preparation method and application thereof, and is used to solve the problem of poor stability of plant vesicles in application in the prior art.

[0006] In the first aspect, the present invention provides a Pickering composition, which comprises the following components, in parts by mass: 6% to 10% moisturizer, 3% to 10% emollient, 0.1% to 0.3% xanthan gum, 2% to 4% microcrystalline cellulose, 8% to 13% anti-inflammatory active component, and the balance is water; wherein, based on the total mass of the Pickering composition, the anti-inflammatory active component includes 3% to 5% rose exosomes, 2% to 4% grape fruit exosomes, and 1% to 4% purslane exosomes.

[0007] As a possible implementation method, the following components are included, in parts by mass: 8% moisturizer, 5% emollient, 0.3% xanthan gum, 4% microcrystalline cellulose, 13% anti-inflammatory active component, and the balance is water; wherein, based on the total mass of the Pickering composition, the anti-inflammatory active component includes 5% rose exosomes, 4% grape fruit exosomes, and 4% purslane exosomes.

[0008] As a possible implementation method, the moisturizer is any one or a combination of butylene glycol, sodium hyaluronate, glycerin, and glyceryl polyether-26; and / or the emollient is any one or a combination of diethylhexyl carbonate, polydimethylsiloxane, caprylic / capric triglyceride, cetyl ethylhexanoate, white meadowfoam seed oil, and vitamin E.

[0009] As a possible implementation method, the particle size of the anti-inflammatory active component is 100 to 200 nm.

[0010] As a possible implementation, it can withstand temperatures of -18 to 50°C, light intensity of 0 to 4500 lx, rotation speed of 0 to 4000 rpm, and storage time of at least 3 months.

[0011] In a second aspect, the present invention provides a method for preparing the Pickering composition described in any possible implementation of the first aspect, comprising the steps of: mixing the moisturizer, the microcrystalline cellulose, the xanthan gum and an appropriate amount of purified water, heating and stirring to dissolve until transparent, keeping warm, and obtaining an aqueous phase mixture; heating the emollient under stirring conditions, stirring until all the oil and fat are dissolved, and obtaining an oil phase; adding the oil phase to the aqueous phase mixture, performing a homogenous stirring operation, cooling to 40-45°C, and obtaining a homogenous mixture; mixing the anti-inflammatory active components evenly, adding them to the homogenous mixture, and stirring to obtain the Pickering composition.

[0012] As a possible implementation method, during the preparation of the aqueous phase mixture, the temperature is raised to 75°C and kept warm for 25 minutes; and / or, during the preparation of the oil phase, the emollient is heated to 70-80°C under stirring conditions, and the stirring rate is based on slight shaking of the liquid surface; and / or, the oil phase is added to the aqueous phase mixture, and the conditions for the homogenous stirring operation are a temperature of 70-75°C, a frequency of 50Hz, and a duration of 10 minutes; and / or, the anti-inflammatory active components are mixed evenly and added to the homogenous mixture, and the stirring rate is based on slight shaking of the liquid surface, and the stirring time is 35 minutes.

[0013] In a third aspect, the present invention provides a use of the Pickering composition described in any possible implementation of the first aspect or the Pickering composition prepared by the preparation method described in any possible implementation of the second aspect in preparing a skin care product.

[0014] As a possible implementation method, the skin care product is used for anti-inflammation and anti-aging.

[0015] The present invention uses Pickering emulsion to stabilize the emulsion system with solid particles, protects the plant vesicle structure, and achieves dual regulation of inflammation and aging.

[0016] The patented invention utilizes Pickering emulsification technology to protect the vesicle structure. Micellar particles are adsorbed on the surface of plant vesicles to form a physical barrier, which effectively protects the plant vesicles from external environmental factors, prevents them from rupturing and aggregation, and protects the integrity of the plant vesicle structure. It can also improve the stability of the composition. By adding xanthan gum, the formation of the Pickering structure is promoted, and the interaction between it and the plant vesicles is enhanced, so that the Pickering composition has good stability. The loss of active ingredients in the plant vesicles is reduced, and the anti-inflammatory and aging effects are enhanced. The antioxidant and anti-inflammatory active ingredients encapsulated inside the plant vesicles can exist stably under the protection of starch granules and are slowly released. These active ingredients can effectively scavenge free radicals in the skin, inhibit the production of inflammatory factors, promote the metabolism of skin cells, and enhance the skin's self-repair ability, thereby significantly enhancing the anti-inflammatory and aging effects.

[0017] Microcrystalline cellulose extracted from naturally derived Sargassum can form an emulsifier-free composition. The emulsifier-free naturally derived microcrystalline cellulose particles are adsorbed on the surface of plant vesicles, forming a physical barrier to protect the plant exosome vesicles and stably exist in the preparation, which not only reduces the irritation caused by emulsifiers to the skin, but also can repair the skin barrier. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 This is a SEM schematic diagram of composition A provided in an embodiment of the present invention.

[0020] Figure 2 This is a SEM schematic diagram of composition D provided in an embodiment of the present invention.

[0021] Figure 3 This is a SEM schematic diagram of composition F provided in an embodiment of the present invention.

[0022] Figure 4 This is a microstructural characterization diagram of composition A provided in an embodiment of the present invention under a confocal microscope.

[0023] Figure 5 This is a microstructure image (10×) of composition A provided in an embodiment of the present invention under an optical microscope.

[0024] Figure 6 This is a microstructure image (4×) of composition A provided in an embodiment of the present invention under an optical microscope.

[0025] Figure 7 This is a structural characterization diagram of composition A provided in an embodiment of the present invention under TEM.

[0026] Figure 8 This is a microstructure diagram of composition F provided in an embodiment of the present invention under an optical microscope.

[0027] Figure 9 This is a microstructure diagram of composition G provided in an embodiment of the present invention under an optical microscope.

[0028] Figure 10 This is the double-layer membrane vesicle structure of composition A provided in an embodiment of the present invention under SEM after acceleration at 45°C for 3M.

[0029] Figure 11 These are the test results of changes in the filaggrin (FLG) content of composition A and composition E provided in the examples of the present invention.

[0030] Figure 12 These are the test results of changes in the loricrin (LOR) content of composition A and composition E provided in the examples of the present invention.

[0031] Figure 13 These are the test results of the changes in the content of pro-inflammatory factors (IL-1α) in Composition A and Composition E provided in the examples of the present invention.

[0032] Figure 14 These are the test results of the changes in the content of inflammatory mediators (PGE2) in Composition A and Composition E provided in the examples of the present invention. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] In order to solve the problem of poor stability of plant vesicles in applications in the prior art, the embodiments of the present invention provide a preparation experiment of a Pickering emulsion composition that protects the vesicle structure, a physical property testing experiment of the composition, and an application experiment of the composition.

[0035] The present invention relates to a composition comprising naturally derived microcrystalline cellulose and xanthan gum, which can form a composite three-dimensional network structure in solution through hydrogen bonding and electrostatic interactions. The high viscosity of xanthan gum and the crystalline properties of microcrystalline cellulose work together to enhance the mechanical strength and stability of the system, forming a Pickering emulsion. This allows droplets to share colloidal particles, forming a three-dimensional network gel structure. Plant exosomes containing anti-inflammatory and senescent properties are encapsulated within this three-dimensional network structure. The three-dimensional network structure of the hydrogel restricts the movement of the exosome vesicles, reducing collisions and aggregation between vesicles. The hydrogel provides a moist environment for the exosome vesicles, preventing them from rupturing under dry conditions. This improves the long-term stability and efficacy of the composition, protects the vesicle structure, maximizes the anti-inflammatory and antioxidant effects of the plant exosomes, and enhances the stability of the composition. A physical barrier is formed to protect the plant exosome vesicles and ensure their stability within the formulation.

[0036] The technical solution of the present invention will be further described below with reference to specific embodiments.

[0037] Example 1

[0038] This example provides an experiment for preparing a Pickering composition.

[0039] Moisturizer (butylene glycol 3%, sodium hyaluronate 0.1%, glycerin 4.9%) 8%, emollient (diethylhexyl carbonate 2.5%, polydimethylsiloxane 2.5%) 5%, xanthan gum 0.3%, microcrystalline cellulose 4%, anti-inflammatory active ingredients (rose exosomes 5%, grape fruit exosomes 4%, purslane exosomes 4%) 13%, and the balance is water. Mix a moisturizer, microcrystalline cellulose, xanthan gum and an appropriate amount of purified water, heat to 75°C, stir and dissolve until transparent, and keep warm for 25 minutes to obtain an aqueous phase mixture; heat the emollient to 70-80°C under stirring conditions, and stir at a rate that allows the liquid surface to shake slightly, and stir until all the oil and fat are dissolved to obtain an oil phase; add the obtained oil phase to the aqueous phase mixture, perform homogenous stirring at 70-75°C, homogenize at 50Hz for 10 minutes, and cool to 40-45°C to obtain a homogenous mixture; mix the anti-inflammatory active components and add them to the obtained homogenous mixture, adjust the speed to allow the liquid surface to shake slightly, and stir for 35 minutes to obtain composition A.

[0040] Moisturizer (butylene glycol 3.5%, sodium hyaluronate 0.05%, glycerin 3.45%) 7%, emollient (diethylhexyl carbonate 1.5%, caprylic / capric triglyceride 1.5%) 3%, xanthan gum 0.2%, microcrystalline cellulose 4%, anti-inflammatory active ingredients (rose exosomes 3%, grape fruit exosomes 3%, purslane exosomes 2%) 8%, and the balance is water. Mix a moisturizer, microcrystalline cellulose, xanthan gum and an appropriate amount of purified water, heat to 65°C, stir and dissolve until transparent, and keep warm for 25 minutes to obtain an aqueous phase mixture; heat the emollient to 70-80°C under stirring conditions, and stir at a rate that allows the liquid surface to shake slightly, and stir until all the oil is dissolved to obtain an oil phase; add the obtained oil phase to the aqueous phase mixture, perform homogenous stirring at 65-70°C, homogenize at 70Hz for 8 minutes, and cool to 40-45°C to obtain a homogeneous mixture; mix the anti-inflammatory active components and add them to the obtained homogeneous mixture, adjust the speed to allow the liquid surface to shake slightly, and stir for 35 minutes to obtain composition B.

[0041] Moisturizer (glyceryl polyether-261.5%, sodium hyaluronate 0.2%, glycerin 3.5%, butylene glycol 4.8%) 10%, emollient (cetyl ethylhexanoate 3%, white meadowfoam seed oil 2%, vitamin E 0.3%, polydimethylsiloxane 4.7%) 10%, xanthan gum 0.25%, microcrystalline cellulose 3%, anti-inflammatory active ingredients (rose exosomes 4%, grape fruit exosomes 4%, purslane exosomes 1%) 9%, and the balance is water. Mix a moisturizer, microcrystalline cellulose, xanthan gum and an appropriate amount of purified water, heat to 70°C, stir and dissolve until transparent, and keep warm for 25 minutes to obtain an aqueous phase mixture; heat the emollient to 70-80°C under stirring conditions, and stir at a rate that allows the liquid surface to shake slightly, and stir until all the oil and fat are dissolved to obtain an oil phase; add the obtained oil phase to the aqueous phase mixture, perform homogenous stirring at 60-65°C, homogenize at 70Hz for 10 minutes, and cool to 40-45°C to obtain a homogeneous mixture; mix the anti-inflammatory active components and add them to the obtained homogeneous mixture, adjust the speed to allow the liquid surface to shake slightly, and stir for 35 minutes to obtain composition C.

[0042] Moisturizer (butylene glycol 3%, sodium hyaluronate 0.15%, glycerin 2.85%) 6%, microcrystalline cellulose 3%, xanthan gum 0.1%, microcrystalline cellulose 2%, anti-inflammatory active ingredient (rose exosomes 3%, grape fruit exosomes 2%, purslane exosomes 3%) 8%, the balance being water. The moisturizer, microcrystalline cellulose, and xanthan gum were mixed with an appropriate amount of purified water, heated to 65°C, stirred and dissolved until transparent, and kept warm for 25 minutes to obtain an aqueous phase mixture. The obtained aqueous phase mixture was homogenized and stirred at 55-60°C, homogenized at 70 Hz for 10 minutes, and cooled to 40-45°C to obtain a homogenous mixture. The anti-inflammatory active ingredients were mixed and added to the obtained homogenous mixture. The speed was adjusted to allow the liquid surface to slightly shake, and stirred for 35 minutes to obtain composition D.

[0043] The difference from the preparation process of composition A is that rose exosomes, grape fruit exosomes and purslane exosomes are not added, and the rest are the same, to obtain composition E.

[0044] The difference from the preparation process of composition A is that no microcrystalline cellulose is added, and the rest are the same, to obtain composition F.

[0045] The difference from the preparation process of composition A is that xanthan gum is not added, and the rest are the same, to obtain composition G.

[0046] The difference from the preparation process of composition A is that the microcrystalline cellulose is replaced by the same mass of titanium dioxide, and the rest are the same, to obtain composition H.

[0047] Example 2

[0048] This embodiment provides a performance test experiment of a Pickering composition.

[0049] 10 g of the sample to be tested was kept at 40° C. for 1 hour and then centrifuged at 2000 rpm, 3000 rpm, and 4000 rpm, respectively. In this example, compositions A to H obtained in Example 1 were used as the test samples for testing, and the results shown in Table 1 were obtained.

[0050] Table 1 Centrifugal test results

[0051] sample Discharging status 2000rpm 3000rpm 4000rpm Composition A normal normal normal normal Composition B normal normal normal normal Composition C normal normal normal normal Composition D normal normal normal normal Composition E normal normal normal normal Composition F normal Oil Demulsification Demulsification Composition G normal Oil Demulsification Demulsification Composition H normal Oil Demulsification Demulsification

[0052] As can be seen from Table 1, compositions A to E have good stability and can withstand a rotation speed of 4000 rpm; compositions F to H have poor stability and cannot withstand a rotation speed of 2000 rpm. Comparison of composition A and composition F to G shows that the compounding of xanthan gum and microcrystalline cellulose with other components can significantly improve the stability of the composition; this is because after microcrystalline cellulose and xanthan gum are compounded, the preparation into a Pickering emulsion can improve the stability of the Pickering micelle structure formed by the Pickering emulsion, thereby improving the stability of plant exosome vesicles in the three-dimensional network structure formed by xanthan gum and Pickering, retaining the effective concentration in the formula, and better exerting its anti-inflammatory, soothing and antioxidant effects. Comparison of composition A and composition H shows that in the composition provided by the present invention, the emulsification stabilization effect of microcrystalline cellulose is better than that of titanium dioxide.

[0053] 100 g of the sample to be tested was placed in different environments [influencing factor experiment: high temperature (50°C), light (illuminance: 4500 lx), cold resistance (-18°C); accelerated experiment: 45°C; long-term experiment: room temperature (25°C)] to test its stability. In this example, compositions A to H obtained in Example 1 were used as test samples for testing, and the results shown in Table 2 were obtained.

[0054] Table 2 Results of extreme environmental stability test of samples (pH / stratification)

[0055]

[0056]

[0057] As shown in Table 2, compositions A to E have good stability and can withstand extreme environments under different conditions, while compositions F to H have poor stability and exhibit varying degrees of stratification under different extreme conditions. Comparison of composition A and composition F to G shows that the compounding of xanthan gum and microcrystalline cellulose with other components can significantly improve the stability of the composition; this is because after compounding microcrystalline cellulose and xanthan gum, the preparation of a Pickering emulsion can improve the stability of the Pickering micelle structure formed by the Pickering emulsion, thereby improving the stability of plant exosome vesicles in the three-dimensional network structure formed by xanthan gum and Pickering, retaining the effective concentration in the formula, and better exerting its anti-inflammatory, soothing, and antioxidant effects. Comparison of composition A and composition H shows that in the composition provided by the present invention, the emulsification stabilization effect of microcrystalline cellulose is better than that of titanium dioxide.

[0058] After 100 g of the test sample was stored at -18°C, 4°C, 45°C, and 50°C for three months, its stability was accelerated at different rotation speeds. In this example, compositions A to H obtained in Example 1 were used as test samples, and the results are shown in Table 3.

[0059] Table 3 Sample centrifugation test

[0060] sample Material state 2000rpm 3000rpm 4000rpm Composition A normal normal normal normal Composition B normal normal normal normal Composition C normal normal normal normal Composition D normal normal normal normal Composition E normal normal normal normal Composition F Slight delamination Oil Oil discharge and sedimentation Oil discharge and sedimentation Composition G Slight delamination Oil Oil discharge and sedimentation Oil discharge and sedimentation Composition H Slight delamination Oil Oil discharge and sedimentation Oil discharge and sedimentation

[0061] As shown in Table 3, compositions A to E have good stability and can withstand a centrifugal speed of 4000 rpm after being stored for 3M under different temperature conditions. Compositions F to H have poor stability and have different degrees of oiling or stratification at different speeds after being stored for 3M under different temperature conditions. Comparison of composition A and composition F to G shows that the compounding of xanthan gum and microcrystalline cellulose with other components can significantly improve the stability of the composition; this is because after compounding microcrystalline cellulose and xanthan gum, the preparation into a Pickering emulsion can improve the stability of the Pickering micelle structure formed by the Pickering emulsion, thereby improving the stability of plant exosome vesicles in the three-dimensional network structure formed by xanthan gum and Pickering, retaining the effective concentration in the formula, and better exerting its anti-inflammatory, soothing and antioxidant effects. Comparison of composition A and composition H shows that in the composition provided by the present invention, the emulsification stabilization effect of microcrystalline cellulose is better than that of titanium dioxide.

[0062] Example 3

[0063] This example provides an experiment to test the stability of the vesicle structure of a Pickering composition.

[0064] Composition A, composition D and composition F were photographed using a transmission electron microscope (TEM) and a scanning electron microscope (SEM) using a negative staining technique, and the following were obtained: Figures 1 to 3 The results are shown. It can be seen that Compositions A and D use a three-dimensional network micelle particle structure that can protect the vesicle structure. Under negative staining technology, a large number of regular and uniform double-layer membrane vesicles with a particle size of 100-200 nm can be observed, which is the vesicle structure of normal plant exocrine extracts. Composition F does not contain the emulsifier of microcrystalline cellulose Pickering technology. Under transmission electron microscopy, the vesicle structure is damaged, the regularity is incomplete, and the contents have leaked.

[0065] The microstructure characterization of composition A under confocal microscope is shown in FIG. Figure 4 As shown, the microstructure of composition A under an optical microscope is shown in FIG. Figure 5 (10×) and Figure 6 As shown in (4×), under a confocal microscope, a large number of spherical solid particles can be seen adsorbed at the oil-water interface of the composition prepared by combining microcrystalline cellulose and xanthan gum, forming a stable three-dimensional network interface film and a stable emulsion system. A large number of spherical micelle particles are dispersed in the emulsion interface, and the system is stable.

[0066] The structural characterization diagram of composition A taken under a scanning electron microscope (TEM) is shown in FIG. Figure 7As shown, it can be seen that a large number of solid particles are adsorbed on the interface surface to form a stable spherical particle structure.

[0067] The microstructures of composition F and composition G under a microscope are shown as follows: Figure 8 and Figure 9 As shown, the composition without microcrystalline cellulose exhibits no solid spherical particles under a microscope, but rather a large number of droplets dispersed at the water-oil interface, forming a relatively stable system. This indicates that the use of xanthan gum or microcrystalline cellulose alone fails to utilize the hydrogen bonding between xanthan gum and microcrystalline cellulose to synergistically form a stable three-dimensional network structure, failing to form a Pickering structure and protecting the plant exosome vesicle structure, thus compromising its stability. It can be seen that the plant exosome vesicle structure of composition G is destroyed after 2 minutes of storage, with the bilayer phospholipid membrane ruptured, thus failing to exert its anti-inflammatory, soothing, and antioxidant effects.

[0068] SEM images of composition A after 3M acceleration under different conditions are shown in Figure 2. Figure 10 As shown, it can be seen that the double-layer membrane vesicle structure is regular and uniform, with a particle size of 100 to 200 nm. It can be seen that the three-dimensional network gel structure formed by composition A can protect the long-term storage of the plant exosome vesicle structure, protect it from rupture, aggregation, sedimentation and other phenomena during the acceleration process, and protect the trace amino acids and protein nucleic acid molecules in the exosome vesicles from being degraded, thereby exerting anti-inflammatory, antioxidant and soothing effects.

[0069] Example 4

[0070] This embodiment provides an application effect experiment of the Pickering composition.

[0071] This embodiment uses SLS to stimulate the 3D epidermal skin model An in vitro skin damage model was constructed, and the repair efficacy of the test sample was evaluated by detecting the changes in tissue vitality, tissue morphology, loricrin (LOR) content, and filaggrin (FLG) content of the skin model after the action of the composition; the soothing efficacy of the test sample was evaluated by detecting the changes in the content of pro-inflammatory factors (IL-1α) and inflammatory mediators (PGE2).

[0072] The anionic surfactant sodium lauryl sulfate (SLS) has amphiphilic (hydrophilic and lipophilic) characteristics. When it comes into contact with the skin at a high concentration, it can damage the skin barrier, especially the lipid components and cell membranes in the barrier, further damaging the cell membranes of living cells and releasing large amounts of IL-1α. IL-1α then activates NFκB to release more pro-inflammatory factors, thereby amplifying the inflammatory response cascade. PGE2 is a prostaglandin and a metabolite of arachidonic acid. Its main function is to induce an inflammatory response. The PGE2 content decreases after the test substance acts, indicating that it can achieve a certain soothing effect. Therefore, the soothing effect can be achieved by inhibiting the pro-inflammatory factor IL-1α and the inflammatory mediator PGE2. External stimulation of skin tissue damages the skin barrier, further damaging living cells and leading to decreased tissue viability. Therefore, changes in tissue viability are a key indicator for assessing the extent of damage and mitigation. Histomorphology, an analysis of the microscopic physiological structure of tissue after HE staining, can reveal changes in the skin barrier under different treatment conditions. For example, after SLS damage, the skin barrier becomes looser and the thickness of the living cell layer decreases, whereas treatment with active ingredients can ameliorate this damage. Loricrin (LOR) is a key component in the assembly of the protein envelope (CE), accounting for approximately 80% of the CE content and reinforcing the skin barrier. A decrease in its content is a major factor in weakened skin barrier function. Flg, a key component in the CE assembly process, is not only a structural component of the skin barrier but can also be hydrolyzed by caspase-14 to form a natural moisturizing factor, thus contributing to moisturizing properties. Therefore, by measuring changes in loricrin (LOR), filaggrin (FLG), TNF-α, and IL-8 levels in the skin model after sample treatment, the repair and anti-inflammatory efficacy of the composition can be evaluated.

[0073] In this example, composition A and composition E were used as test objects to detect barrier-related proteins (FLG & LOR) and inflammatory factors (IL-1α & PGE2), and the results were as follows: Figures 11 to 14 The results shown are as follows, where BC represents blank control and NC represents negative control.

[0074] Depend on Figure 11 As can be seen, compared with the BC group, the FLG protein content in the NC group decreased significantly, indicating that the stimulation conditions in this test were effective. Compared with the NC group, the FLG protein content in Composition E increased significantly, and the FLG protein content in Composition A increased significantly, and the increase in Composition A was greater than that in Composition E, demonstrating that Composition A has a more significant skin repair effect.

[0075] Depend on Figure 12As can be seen, compared with the BC group, the LOR protein content in the NC group decreased significantly, indicating that the stimulation conditions of this test were effective. Compared with the NC group, the LOR protein content in Composition E increased significantly, and the LOR protein content in Composition A increased significantly, and the increase in Composition A was greater than that in Composition E, demonstrating that Composition A has a more significant skin repair effect.

[0076] Depend on Figure 13 As can be seen, compared to the BC group, the NC group had significantly higher levels of TNF-α secretion, indicating that the stimulation conditions tested were effective. Compared to the NC group, Composition E inhibited SLS-induced TNF-α secretion, while Composition A significantly inhibited TNF-α secretion, with Composition A exhibiting a higher inhibitory effect than Composition E, demonstrating more significant soothing and anti-inflammatory effects on the skin.

[0077] Depend on Figure 14 As can be seen, compared to the BC group, the NC group had significantly higher IL-8 secretion levels, indicating that the stimulation conditions tested were effective. Compared to the NC group, Composition E inhibited SLS-induced IL-8 secretion, while Composition A significantly inhibited IL-8 secretion, with Composition A exhibiting a higher inhibitory effect than Composition E, demonstrating more significant soothing and anti-inflammatory effects on the skin.

[0078] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0079] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A Pickering composition, characterized in that Calculated by mass, it includes the following components: Moisturizer 6% to 10%, emollient 3% to 10%, xanthan gum 0.1% to 0.3%, microcrystalline cellulose 2% to 4%, anti-inflammatory active ingredient 8% to 13%, and the balance is water; Wherein, based on the total mass of the Pickering composition, the anti-inflammatory active components include 3% to 5% of rose exosomes, 2% to 4% of grape fruit exosomes, and 1% to 4% of purslane exosomes.

2. The Pickering composition according to claim 1, wherein Calculated by mass, it includes the following components: Moisturizer 8%, emollient 5%, xanthan gum 0.3%, microcrystalline cellulose 4%, anti-inflammatory active ingredient 13%, the balance is water; Wherein, based on the total mass of the Pickering composition, the anti-inflammatory active components include 5% rose exosomes, 4% grape fruit exosomes, and 4% purslane exosomes.

3. The Pickering composition according to claim 1, wherein The moisturizing agent is any one or a combination of butylene glycol, sodium hyaluronate, glycerin, and glyceryl ether-26; And / or, the emollient is any one or a combination of diethylhexyl carbonate, dimethicone, caprylic / capric triglyceride, cetyl ethylhexanoate, meadowfoam seed oil, and vitamin E.

4. The Pickering composition according to claim 1, characterized in that The particle size of its anti-inflammatory active component is 100-200nm.

5. The Pickering composition according to claim 1, characterized in that The device can withstand temperatures ranging from -18 to 50°C, light intensity ranging from 0 to 4500 lx, rotation speed ranging from 0 to 4000 rpm, and storage time of at least 3 months.

6. The method for preparing the Pickering composition according to any one of claims 1 to 5, characterized in that: Including steps: Mixing the moisturizing agent, the microcrystalline cellulose, the xanthan gum and an appropriate amount of purified water, heating and stirring to dissolve until transparent, and keeping the temperature to obtain an aqueous phase mixture; The emollient is heated under stirring conditions and stirred until the oil is completely dissolved to obtain an oil phase; Add the oil phase to the aqueous phase mixture, perform homogenous stirring, and cool to 40-45° C. to obtain a homogenous mixture; The anti-inflammatory active components are mixed evenly and added into the homogeneous mixed solution, and stirred to obtain the Pickering composition.

7. The preparation method according to claim 6, characterized in that During the preparation of the aqueous phase mixture, the temperature is raised to 65-75°C and kept warm for 25 minutes; And / or, during the preparation of the oil phase, the emollient is heated to 70-80° C. under stirring, and the stirring rate is such that the liquid surface shakes slightly; And / or, the oil phase is added to the aqueous phase mixture, and the homogenizing stirring operation conditions are a temperature of 60-75° C., a frequency of 50 Hz, and a duration of 10 minutes; And / or, the anti-inflammatory active components are mixed evenly and then added to the homogeneous mixed solution, with a stirring rate such that the liquid surface shakes slightly and the stirring time is 35 minutes.

8. Use of the Pickering composition according to any one of claims 1 to 5 or the Pickering composition prepared by the preparation method according to any one of claims 6 to 7 in the preparation of skin care products.

9. The use according to claim 8, characterized in that The skin care product is used for anti-inflammation and anti-aging.

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