Mosquito-repelling, bacterium-inhibiting and itching-relieving composition containing osmanthus fragrans extract and preparation method and application of mosquito-repelling, bacterium-inhibiting and itching-relieving composition

Abstract

The invention discloses a mosquito-repelling, bacterium-inhibiting and itching-relieving composition containing a sweet-scented osmanthus extract and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The mosquito-repelling, bacterium-inhibiting and itching-relieving composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of sweet-scented osmanthus extract, 15 to 25 parts of lepidium sativum bud extracting solution, 10 to 18 parts of mistletoe extract, 7 to 12 parts of garden burnet root extract, 5 to 12 parts of wormwood, 5 to 10 parts of mint, 4 to 10 parts of honeysuckle flower, 3 to 8 parts of raspberry ketone glucoside, 2 to 6 parts of glycyrrhetinic acid and 100 to 300 parts of water. The raw materials of the mosquito-repelling, bacterium-inhibiting and itching-relieving composition are all from plant components, so that the mosquito-repelling, bacterium-inhibiting and itching-relieving composition is safe to use, free of side effects, capable of rapidly achieving mosquito-repelling and itching-relieving effects and good in bacterium-inhibiting effect.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a mosquito-repellent, antibacterial and itching-relieving composition containing osmanthus fragrans extract, and a preparation method and application thereof. Background Art

[0002] Currently, there are a wide variety of mosquito repellent products on the market, such as traditional mosquito coils, electric mosquito coils, and electric mosquito coil liquids. These all contain pyrethroid chemical insecticides that produce smoke when used. These chemical mosquito repellents are highly toxic. Long-term and frequent use not only harms human health and reduces human immunity, but also pollutes the environment with the smoke they produce, causing adverse effects on the respiratory system and causing discomfort. There are also many mosquito repellent and antipruritic products on the market, such as floral water and mosquito repellent antipruritic creams. Most mosquito repellent floral waters contain the chemically synthesized repellents DEET or pyrethroid, which are irritating and toxic. However, with the continuous improvement of modern living standards and people's safety awareness, the current various mosquito repellent products cannot meet the needs of consumers. People are increasingly seeking safe, natural, non-toxic, and highly effective mosquito repellent and antipruritic products. At the same time, it has been found that products with mosquito repellent and antipruritic effects in the prior art do not necessarily have antibacterial effects, resulting in a single product efficacy that cannot fully meet people's daily needs. How to formulate a safe, non-toxic, and long-lasting mosquito repellent, antibacterial, and antipruritic composition is a technical problem that has long been urgently needed by those skilled in the art. Summary of the Invention

[0003] In view of the above-mentioned defects of the prior art, the present invention proposes a mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract, a preparation method thereof and an application cream to solve the problems raised by the above-mentioned background technology.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 20-30 parts of osmanthus extract, 15-25 parts of Lepidium sativum Linnaeus bud extract, 10-18 parts of Viscum album L. extract (the leafy stems of Viscum ovata, a plant of the Morus altissima family), 7-12 parts of Sanguisorba officinalis Linn. root extract, 5-12 parts of mugwort, 5-10 parts of peppermint, 4-10 parts of honeysuckle, 3-8 parts of raspberry ketone glucoside, 2-6 parts of glycyrrhizic acid and 100-300 parts of water.

[0006] Preferably, a mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of Sanguisorba officinalis root extract, 10 parts of mugwort, 9 parts of mint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

[0007] Preferably, the preparation method of osmanthus extract comprises the following steps:

[0008] (1) Dry and crush the osmanthus flowers, add anhydrous ethanol in a ratio of 1 g to (10-25) mL, and extract at a temperature of 30-40° C. and an ultrasonic power of 450-500 W for 40-60 min;

[0009] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, the pH of the supernatant was adjusted to 4.5-6.0 with a pH regulator, the supernatant was allowed to stand for 25-35 minutes, and then centrifuged. The extract was collected and evaporated and concentrated to obtain the osmanthus extract;

[0010] Preferably, the pH regulator in step (2) is citric acid or sodium citrate.

[0011] Preferably, the preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a ratio of 1 g: (20-30) mL for ultrasonic treatment for 30-40 minutes, then adding cellulase and ligninase for enzymolysis for 20-25 minutes, inactivating the enzymes after the enzymolysis is completed, and centrifuging to obtain the Lepidium japonicum sprout extract.

[0012] Preferably, the mass ratio of Lepidium sativum sprouts, cellulase and ligninase is 20:(0.4-0.9):(0.1-0.6).

[0013] Preferably, the cellulase activity is ≥11,000 U / g, and the ligninase activity is ≥10,000 U / g.

[0014] Preferably, the preparation method of the ginkgo mistletoe extract includes the following steps: evenly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of (1 to 3):1, performing heating reflux extraction at a temperature of 65 to 85°C for 100 to 200 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0015] Preferably, a method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following steps:

[0016] S1. Wash wormwood, mint and honeysuckle, dry and crush them, extract them with supercritical carbon dioxide at a temperature of 40-50° C. and an extraction pressure of 25-40 MPa for 2.0-4.0 h, and collect the volatile oil for later use;

[0017] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 45-55° C., stir and mix for 8-12 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 15-22 minutes, let stand for 15-20 seconds, and obtain a mixture for later use;

[0018] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 30 to 40 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0019] Preferably, the carbon dioxide flow rate in step S1 is 25 to 32 L / h.

[0020] Preferably, the mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract involved in the present application is used in the preparation of products with the effects of inhibiting Staphylococcus aureus, Candida albicans, Corynebacterium diphtheriae, Trichophyton mentagrophytes and Streptococcus pyogenes, as well as relieving itching and repelling mosquitoes.

[0021] Preferably, the mosquito repellent, antibacterial and antipruritic composition may be in the form of a solid or liquid.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] This invention combines osmanthus extract, Lepidium sativum sprout extract, ginkgo mistletoe extract, sanguisorba officinalis root extract, wormwood, mint, honeysuckle, raspberry ketone glucoside, and glycyrrhetinic acid to achieve mosquito repellent, antibacterial, and antipruritic effects, with test results superior to existing commercially available products. The mosquito repellent, antibacterial, and antipruritic composition prepared by the invention can inhibit the growth of five common skin bacteria: Staphylococcus aureus, Candida albicans, Corynebacterium diphtheriae, Trichophyton mentagrophytes, and Streptococcus pyogenes. Its preparation method is simple and the raw materials are readily available. DETAILED DESCRIPTION

[0024] The technical solution of the present invention will be further described in detail below in conjunction with specific implementation methods.

[0025] Example 1

[0026] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 20 parts of osmanthus extract, 15 parts of Lepidium japonicum sprout extract, 10 parts of ginkgo mistletoe extract, 7 parts of sanguisorba officinalis root extract, 5 parts of wormwood, 5 parts of peppermint, 4 parts of honeysuckle, 3 parts of raspberry ketone glucoside, 2 parts of glycyrrhizic acid and 100 parts of water.

[0027] The preparation method of the osmanthus extract comprises the following steps:

[0028] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:10 mL, and the mixture was extracted at a temperature of 30°C and an ultrasonic power of 450 W for 40 min;

[0029] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 4.5 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 25 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain the osmanthus extract.

[0030] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:20 mL, performing ultrasonic treatment for 30 minutes, then adding cellulase and ligninase for enzymolysis for 20 minutes, inactivating the enzymes after the enzymolysis is completed, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.4:0.1.

[0031] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 1:1, performing heating reflux extraction at a temperature of 65° C. for 100 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0032] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0033] S1. Wash wormwood, mint, and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 40°C and an extraction pressure of 25 MPa for 2.0 h at a carbon dioxide flow rate of 25 L / h. Collect the volatile oil and set aside;

[0034] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, heat to 45° C., stir and mix for 8 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 15 minutes, let stand for 15 seconds, and obtain a mixture for later use;

[0035] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 30 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0036] Example 2

[0037] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 30 parts of osmanthus extract, 25 parts of Lepidium japonicum sprout extract, 18 parts of ginkgo mistletoe extract, 12 parts of sanguisorba officinalis root extract, 12 parts of wormwood, 10 parts of mint, 10 parts of honeysuckle, 8 parts of raspberry ketone glucoside, 6 parts of glycyrrhizic acid and 300 parts of water.

[0038] The preparation method of the osmanthus extract comprises the following steps:

[0039] (1) Dry and crush the osmanthus flowers, add anhydrous ethanol at a material-liquid ratio of 1 g:25 mL, and extract at a temperature of 40°C and an ultrasonic power of 500 W for 60 min;

[0040] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 6.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 35 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0041] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:30 mL, performing ultrasonic treatment for 40 minutes, then adding cellulase and ligninase for enzymolysis for 25 minutes, inactivating the enzymes after the enzymolysis is completed, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.9:0.6.

[0042] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 3:1, performing heating reflux extraction at a temperature of 85° C. for 200 minutes, and filtering and rotary evaporating after the extraction to obtain the ginkgo mistletoe extract.

[0043] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0044] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 50°C and an extraction pressure of 40 MPa for 4.0 h at a carbon dioxide flow rate of 32 L / h to collect volatile oil for later use;

[0045] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 55° C., stir and mix for 12 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 22 minutes, let stand for 20 seconds, and obtain a mixture for later use;

[0046] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 40 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0047] Example 3

[0048] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of sanguisorba officinalis root extract, 10 parts of mugwort, 9 parts of peppermint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

[0049] The preparation method of the osmanthus extract comprises the following steps:

[0050] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0051] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0052] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0053] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0054] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0055] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0056] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0057] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0058] Comparative Example 1

[0059] This comparative example differs from Example 3 in that the osmanthus extract is replaced with lotus extract, and the other ingredients and steps remain unchanged.

[0060] A mosquito repellent, antibacterial and antipruritic composition comprises the following raw materials in parts by weight: 25 parts of lotus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of sanguisorba officinalis root extract, 10 parts of mugwort, 9 parts of mint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

[0061] The preparation method of the lotus extract comprises the following steps:

[0062] (1) The lotus flower was dried and crushed, and anhydrous ethanol was added at a solid-liquid ratio of 1 g:20 mL. The mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min.

[0063] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain the lotus extract.

[0064] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0065] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0066] A method for preparing a mosquito repellent, antibacterial and antipruritic composition comprises the following steps:

[0067] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0068] S2. Add lotus extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0069] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0070] Comparative Example 2

[0071] This comparative example differs from Example 3 in that the mosquito repellent, antibacterial and antipruritic composition does not contain the Lepidium sativum sprout extract, and the other ingredients and steps remain unchanged.

[0072] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 17 parts of ginkgo mistletoe extract, 11 parts of sanguisorba officinalis root extract, 10 parts of wormwood, 9 parts of mint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

[0073] The preparation method of the osmanthus extract comprises the following steps:

[0074] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0075] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0076] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0077] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0078] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0079] S2. Add osmanthus extract, ginkgo mistletoe extract, sanguisorba officinalis root extract and water into a blender in sequence, heat to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0080] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0081] Comparative Example 3

[0082] This comparative example differs from Example 3 in that the mosquito repellent, antibacterial and antipruritic composition does not contain the ginkgo mistletoe extract, and the other ingredients and steps remain unchanged.

[0083] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 11 parts of Sanguisorba officinalis root extract, 10 parts of mugwort, 9 parts of mint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

[0084] The preparation method of the osmanthus extract comprises the following steps:

[0085] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0086] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0087] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0088] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0089] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0090] S2. Add osmanthus fragrans extract, Lepidium truncatum sprout extract, Sanguisorba officinalis root extract, and water into a blender in sequence, heat to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0091] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0092] Comparative Example 4

[0093] This comparative example differs from Example 3 in that the Sanguisorba officinalis root extract is replaced with the Cymbopogon citronella extract, and the other ingredients and steps remain unchanged.

[0094] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of citronella extract, 10 parts of wormwood, 9 parts of mint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

[0095] The preparation method of the osmanthus extract comprises the following steps:

[0096] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0097] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0098] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0099] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0100] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0101] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0102] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, citronella extract, and water into a blender in sequence, raise the temperature to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0103] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0104] Comparative Example 5

[0105] This comparative example differs from Example 3 in that the weight parts are changed, while the other ingredients and steps remain unchanged.

[0106] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 50 parts of osmanthus extract, 40 parts of Lepidium japonicum sprout extract, 30 parts of ginkgo mistletoe extract, 20 parts of sanguisorba officinalis root extract, 6 parts of mugwort, 7 parts of peppermint, 6 parts of honeysuckle, 4 parts of raspberry ketone glucoside, 3 parts of glycyrrhizic acid and 200 parts of water.

[0107] The preparation method of the osmanthus extract comprises the following steps:

[0108] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0109] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0110] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0111] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0112] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0113] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0114] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0115] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0116] Comparative Example 6

[0117] This comparative example differs from Example 3 in that raspberry ketone glucoside is replaced with alkyl glucoside, and the other ingredients and steps remain unchanged.

[0118] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of sanguisorba officinalis root extract, 10 parts of wormwood, 9 parts of peppermint, 7 parts of honeysuckle, 6 parts of alkyl glycosides, 4 parts of glycyrrhizic acid and 200 parts of water.

[0119] The preparation method of the osmanthus extract comprises the following steps:

[0120] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0121] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0122] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0123] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0124] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0125] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0126] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 50° C., stir and mix for 10 minutes, then add alkyl glycoside and glycyrrhetinic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0127] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0128] Comparative Example 7

[0129] This comparative example differs from Example 3 in that glycyrrhetinic acid is replaced with salicylic acid, and the other ingredients and steps remain unchanged.

[0130] A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of sanguisorba officinalis root extract, 10 parts of mugwort, 9 parts of peppermint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of salicylic acid and 200 parts of water.

[0131] The preparation method of the osmanthus extract comprises the following steps:

[0132] (1) Osmanthus fragrans was dried and crushed, and anhydrous ethanol was added at a material-liquid ratio of 1 g:20 mL, and the mixture was extracted at a temperature of 35°C and an ultrasonic power of 480 W for 50 min;

[0133] (2) After the ultrasonic treatment, the supernatant was collected by solid-liquid separation, and the pH of the supernatant was adjusted to 5.0 with a pH regulator such as citric acid or sodium citrate. The supernatant was allowed to stand for 30 minutes and then centrifuged. The extract was collected and concentrated by evaporation to obtain an osmanthus extract.

[0134] The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprouts, adding deionized water at a material-liquid ratio of 1 g:25 mL, performing ultrasonic treatment for 35 minutes, then adding cellulase and ligninase for enzymolysis for 22 minutes, inactivating the enzymes after the enzymolysis, and centrifuging to obtain the Lepidium japonicum sprout extract; wherein the mass ratio of the Lepidium japonicum sprouts, cellulase, and ligninase is 20:0.6:0.5.

[0135] The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of 2:1, performing heating reflux extraction at a temperature of 80° C. for 150 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

[0136] A method for preparing a mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract comprises the following steps:

[0137] S1. Wash wormwood, mint and honeysuckle, dry and crush them, and extract them with supercritical carbon dioxide at a temperature of 45°C and an extraction pressure of 30 MPa for 3.0 h at a carbon dioxide flow rate of 30 L / h. Collect the volatile oil and set aside;

[0138] S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, heat to 50° C., stir and mix for 10 minutes, then add raspberry ketone glucoside and salicylic acid, continue stirring for 20 minutes, let stand for 18 seconds, and obtain a mixture for later use;

[0139] S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 35 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

[0140] Experimental Example 1: Mosquito Repellent Experiment

[0141] 1.1 Experimental Materials

[0142] The mosquito repellent, antibacterial and antipruritic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 7; 240 22±2g Kunming mice, Culex pipiens faeces, and commercially available mosquito repellent cream.

[0143] 1.2 Experimental Grouping

[0144] The experimental group used the mosquito repellent, antibacterial and antipruritic compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 7; the positive control group used a commercially available mosquito repellent cream; and the negative control group used physiological saline.

[0145] 1.3 Experimental methods

[0146] 240 Kunming mice (half male and half female) were divided into 12 groups of 20 mice each. The mice's limbs were fixed to a wooden board, and the villi on the abdomen with a diameter of 2 cm were removed. Then, 2.0 uL / cm of the combination / mosquito repellent cream / normal saline used in the corresponding experimental group were evenly applied to the exposed abdomen. The mice were placed in 50×50×50 cm Culex pipiens cages, with 100 Culex pipiens pipiens in each cage. The mice were observed for mosquitoes coming to suck blood. As long as a single mosquito came to suck blood, the protection was considered to be ineffective. The average effective protection time was recorded.

[0147] 1.4 The mosquito repellent results are shown in Table 1:

[0148] Table 1 Average effective protection time of mosquito repellent

[0149] Experimental groups Average effective protection time (h) Example 1 7.51 Example 2 7.84 Example 3 8.05 Comparative Example 1 6.97 Comparative Example 2 5.42 Comparative Example 3 5.10 Comparative Example 4 6.04 Comparative Example 5 5.41 Comparative Example 6 6.72 Comparative Example 7 6.36 Negative control group <0.5 Positive control group 6.75

[0150] As can be seen from Table 1, the average effective protection time of the mosquito repellent, antibacterial and antipruritic compositions provided in Examples 1 to 3 is significantly longer than that of the negative control group and the positive control group, indicating that the mosquito repellent, antibacterial and antipruritic compositions provided by the present invention have a good mosquito repellent effect. The mosquito repellent, antibacterial and antipruritic compositions prepared by using specific raw materials and appropriate amounts of raw materials have a significantly enhanced mosquito repellent effect. The extract of Lepidium sativum sprouts contains volatile components such as phenylacetonitrile, benzyl thiocyanate, and benzyl isothiocyanate. The osmanthus extract, ginkgo mistletoe extract, and other raw materials can release flavonoid glycosides, triterpenoid saponins, and other components, which interfere with the olfactory perception system of mosquitoes, thereby reducing the approach of mosquitoes. There is a synergistic effect between the raw materials, and the mosquito repellent effect is better and better than the existing mosquito repellent cream. The mosquito repellent, antibacterial and antipruritic composition of the present application is formulated with natural Chinese medicinal plants, has no toxic side effects, and is healthier and safer.

[0151] Experimental Example 2 Antibacterial Experiment

[0152] 2.1 Experimental Grouping

[0153] Experimental group: The mosquito repellent, antibacterial and antipruritic compositions obtained in Examples 1 to 3 were prepared into a composition solution, which was injected onto the surface of an LB solid culture medium plate and slowly shaken to evenly distribute the composition solution on the plate surface. The operation was performed under sterile conditions, with an injection dose of 6 ml per plate.

[0154] Control group: The composition solution of the experimental group was replaced with an equal amount of sterile water, and the rest of the operations were the same as those of the experimental group.

[0155] 2.2 Test Method

[0156] Take the concentration as 10 6 ~10 8 cfu / ml of Staphylococcus aureus liquid, Candida albicans liquid, Corynebacterium diphtheriae liquid, Trichophyton mentagrophytes liquid and Streptococcus pyogenes liquid were injected onto the surface of the experimental and control plates, and slowly shaken to mix the liquid with the composition solution or sterile water. The operation was carried out under aseptic conditions, the injection dose was 0.2 ml / plate, and each group was tested 3 times in parallel. The plates were placed in a 37°C constant temperature incubator and cultured. After 48 hours, they were taken out for observation and the growth was recorded. Plates with bacterial growth were recorded as positive (+), and plates without growth were recorded as negative (-).

[0157] 2.3 Antibacterial test results

[0158] As shown in Table 2:

[0159] Table 2 Antibacterial effect

[0160] Experimental groups Example 1 Example 2 Example 3 control group Staphylococcus aureus - - - + Candida albicans - - - + Corynebacterium diphtheriae - - - + Trichophyton mentagrophytes - - - + Streptococcus pyogenes - - - +

[0161] The results showed that the mosquito repellent, antibacterial and antipruritic composition of the present invention has the effect of inhibiting the growth of Staphylococcus aureus, Candida albicans, Corynebacterium diphtheriae, Trichophyton mentagrophytes and Streptococcus pyogenes.

[0162] Experimental Example 3 Antipruritic Experiment

[0163] 3.1 Test Method

[0164] Thirty common guinea pigs, weighing 250±20g, half male and half female, were randomly divided into three groups of 10 each after being routinely raised for 7 days, namely a negative control group (using physiological saline), a test group (using the mosquito repellent, antibacterial and antipruritic composition prepared in Example 3), and a positive control group (using dexamethasone). The composition in each test group was dissolved in sterile water to a concentration of 30mg / ml as the test solution. One day before the test, the dorsum of the right hind paw of each group of guinea pigs was shaved with electric clippers and depilated with 8% sodium sulfide, with an area of about 1cm 2 On the day of the experiment, the depilated skin was gently abraded with 800-grit fine sandpaper until redness was achieved, but no bleeding was observed. 0.05 mL of the corresponding drug solution was applied to the abrasion. Twenty minutes after the drug application, 0.05 mL of 0.5% histamine phosphate solution was dripped onto the wound surface on the dorsum of the right hind paw. The average duration of pruritus was recorded. The itch was indicated by licking the dorsum of the right hind paw. Specific test results are shown in Table 3.

[0165] 3.2 Antipruritic Effects

[0166] Table 3 Average duration of itching

[0167] Experimental groups Average itching duration (s) Example 3 7 Negative control group 92 Positive control group 18

[0168] As can be seen from Table 3, the mosquito repellent, antibacterial and antipruritic composition prepared in the present application has an antipruritic effect.

[0169] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract, characterized in that: The invention comprises the following raw materials in parts by weight: 20 to 30 parts of osmanthus extract, 15 to 25 parts of Lepidium japonicum sprout extract, 10 to 18 parts of ginkgo mistletoe extract, 7 to 12 parts of sanguisorba officinalis root extract, 5 to 12 parts of wormwood, 5 to 10 parts of mint, 4 to 10 parts of honeysuckle, 3 to 8 parts of raspberry ketone glucoside, 2 to 6 parts of glycyrrhetinic acid and 100 to 300 parts of water.

2. The mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 25 parts of osmanthus extract, 20 parts of Lepidium japonicum sprout extract, 17 parts of ginkgo mistletoe extract, 11 parts of sanguisorba officinalis root extract, 10 parts of wormwood, 9 parts of mint, 7 parts of honeysuckle, 6 parts of raspberry ketone glucoside, 4 parts of glycyrrhizic acid and 200 parts of water.

3. The mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 1, characterized in that: The preparation method of osmanthus extract comprises the following steps: (1) Dry and crush the osmanthus flowers, add anhydrous ethanol in a ratio of 1 g to (10-25) mL, and extract at a temperature of 30-40° C. and an ultrasonic power of 450-500 W for 40-60 min; (2) After the ultrasound is completed, the supernatant is collected by solid-liquid separation, and the pH of the supernatant is adjusted to 4.5-6.0 with a pH regulator. After standing for 25-35 minutes, the supernatant is centrifuged and the extract is collected and evaporated to obtain the osmanthus extract.

4. The mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 3, characterized in that: The pH regulator in step (2) is citric acid or sodium citrate.

5. The mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 1, characterized in that: The preparation method of the Lepidium japonicum sprout extract comprises the following steps: drying and crushing the Lepidium japonicum sprout, adding deionized water in a ratio of 1 g: (20-30) mL of solid-liquid to carry out ultrasonic treatment for 30-40 minutes, then adding cellulase and ligninase for enzymolysis for 20-25 minutes, inactivating the enzymes after the enzymolysis is completed, and centrifuging to obtain the Lepidium japonicum sprout extract.

6. The mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 5, characterized in that: The mass ratio of Lepidium sativum sprouts, cellulase and ligninase is 20:(0.4-0.9):(0.1-0.6).

7. The mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 1, characterized in that: The preparation method of the ginkgo mistletoe extract comprises the following steps: uniformly mixing anhydrous ethanol and dried ginkgo mistletoe powder in a mass ratio of (1-3):1, performing heating reflux extraction at a temperature of 65-85°C for 100-200 minutes, and after the extraction, filtering and rotary evaporation to obtain the ginkgo mistletoe extract.

8. The method for preparing the mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to claim 1, characterized in that: The following steps are involved: S1. Wash wormwood, mint and honeysuckle, dry and crush them, extract them with supercritical carbon dioxide at a temperature of 40-50° C. and an extraction pressure of 25-40 MPa for 2.0-4.0 h, and collect the volatile oil for later use; S2. Add osmanthus fragrans extract, Lepidium japonicum sprout extract, ginkgo mistletoe extract, Sanguisorba officinalis root extract, and water into a blender in sequence, raise the temperature to 45-55° C., stir and mix for 8-12 minutes, then add raspberry ketone glucoside and glycyrrhetinic acid, continue stirring for 15-22 minutes, let stand for 15-20 seconds, and obtain a mixture for later use; S3. Add the volatile oil obtained in step S1 to the mixture obtained in step S2 and continue stirring for 30 to 40 minutes to obtain a mosquito repellent, antibacterial and antipruritic composition.

9. The method for preparing the mosquito repellent, antibacterial and antipruritic composition containing osmanthus fragrans extract according to claim 8, characterized in that: The carbon dioxide flow rate in step S1 is 25 to 32 L / h.

10. Use of the mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract according to any one of claims 1 to 7 or the mosquito repellent, antibacterial and antipruritic composition containing osmanthus extract prepared by the preparation method according to any one of claims 8 to 9 in preparing a product having the effects of inhibiting Staphylococcus aureus, Candida albicans, Corynebacterium diphtheriae, Trichophyton mentagrophytes and Streptococcus pyogenes, and relieving itching and repelling mosquitoes.